CN108812125A - A kind of anti-accurate identification method of brown plant-hopper scale of rice seedling - Google Patents
A kind of anti-accurate identification method of brown plant-hopper scale of rice seedling Download PDFInfo
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Abstract
The invention discloses a kind of anti-accurate identification methods of brown plant-hopper scale of rice seedling, belong to rice breeding field.The method that the anti-brown plant-hopper scale of rice seedling of the invention is precisely identified, includes the following steps：The building of large-scale authentication room；Worm is used in raising identification；Extensive rice growing；Excessively connect worm identification；Scientific management after connecing worm；The result and evaluation of Resistance Identification；The transplanting of resistant material.The present invention establishes application of the rice seedling to the accurate identification technology system of the scale of brown plant-hopper Resistance Identification and the technology on anti-brown plant-hopper rice breeding.
Description
Technical field
The invention belongs to rice breeding fields, and in particular to a kind of anti-brown plant-hopper scale of rice seedling is precisely identified
Method.
Background technique
Rice is one of the staple food crop in China, and seriously threatening for brown plant-hopper is faced during Rice Cropping.Rice
Brown paddy plant hopper is a kind of rice grub explosive, harm is big, and the annual rice hoppers in China (mainly brown paddy plant hopper) occur at present
Area is 20,000,000 hectares or more, and direct production loss is up to 2,800,000 tons or more caused by endangering every year because of planthopper.Always with
Come, the prevention and treatment of brown paddy plant hopper is mainly by application chemical insecticide.A large amount of applications of insecticide often lead to the broken of ecological environment
Bad, a large amount of brown paddy plant hopper natural enemy is killed to be improved with the drug resistance of pest, so as to cause " rampant again " of brown paddy plant hopper, gives prevention and treatment band
Carry out bigger difficulty.It facts have proved that cultivating resistant rice varieties is the most economic, effective, safety, life in brown paddy plant hopper integrated control
The method of state.Resistance breeding mainly imports resistant gene into excellent rice breed, and wherein Resistance Identification is resistance
Committed step in breeding, by obtaining reliable and stable resistance offspring, therefrom selecting resistance to breeding progeny Resistance Identification
Rice varieties.
At present both at home and abroad to the general small scale of the Resistance Identification of brown plant-hopper, while Resistance Identification few, the Bu Nengshi that connects worm amount
The needs for answering resistance breeding to identify a large amount of breeding materials, lead to that determination rates are poor, accuracy is poor, and then cause breeding efficiency low
Under, the anti-brown plant-hopper rice varieties ratio cultivated is few.Current representational rice seedling brown plant-hopper identification technology master
The publication of the Jiangxi Yao You Administration of Quality and Technology Supervision of Shanxi Province《Rice Resistance Brown Planthopper Resistance identification technology regulation》[Jiangxi Province's provincial standard,
BD36, (exposure draft)] and document《Comparative studies of the rice to planthopper Resistance Identification》(Chen Ying etc., Agriculture of Anhui section
Learn, 2010), feature is all test material sowing in plastics or metal basin, cover upper guard when identification, disposable expert evidence
Quantity is few, connects that worm amount is few, and the reliability of qualification result is poor, and the resistance for not adapting to extensive rice breeding is precisely identified and needed
It wants.Therefore, it is brown to rice winged to adapt to the accurate identification technology system of brown plant-hopper scale that extensive rice breeding needs for foundation
Lice resistance breeding has important practical significance and application prospect.
Summary of the invention
The purpose of the present invention is establishing rice seedling to the accurate identification technology system of the scale of brown plant-hopper Resistance Identification,
Make application of the technology on anti-brown plant-hopper rice breeding, improves rice breeding efficiency.
The purpose of the present invention is what is be achieved through the following technical solutions：
A kind of method that the anti-brown plant-hopper scale of rice seedling is precisely identified, includes the following steps：
(1) building of large-scale authentication room, large-scale authentication room include glass caping, sunshade net, stainless (steel) wire, fly net, push away
Sliding door and window, planting bed, automatic irrigation system；Stainless (steel) wire is located at the surrounding of authentication room, is equipped with insect prevention in the inside of stainless (steel) wire
Net forms double shielding；The surrounding of authentication room is provided with sliding door and window to be opened/closed；Sunshade net is arranged under glass caping
Side, sunshade net light transmittance are 20-40%, and sunshade net movable can be opened and closed；Planting bed is uniformly neatly arranged in authentication room, kind
It is 2.4-3.0 meters a length of to plant pond, width is 1.2-1.5 meters, and depth is 0.5-0.8 meters；Husky loamy texture rice soil is filled in it；Automatic irrigation system
System is connected with each planting bed；The area of authentication room is 100-200 square metres, is highly 3-5 meters；
(2) worm is used in raising identification；
Brown plant-hopper worm sources select local field worm sources, at the beginning of early season worm sources annual 3 months~4 months in field or the wild rice of passing the winter
Field capture, late season worm sources are then captured in mid-August from field；The worm sources of capture supporting with the sealing with fly net in insectary
The raising of worm cage；The egg-laying season adult that puberty neat and consistent is chosen in next-generation worm carries out mass propagation, obtains for 1~2 age
Nymph connects worm Resistance Identification for rice；
(3) extensive rice growing；
Prepare before sowing：10-15 days before sowing, carried out disinfection with insecticide to planting bed and authentication room space；Planting bed
It poured water, harrow rotten, making ridge into smooth sowing seedbed, surrounding stays ditch to facilitate and irrigate；
Sowing：The rice material of every part of F3 or more generation broadcasts 40-60 vernalization seeds, and F2 broadcasts 500 for separation material
Above seed；Every part of material broadcasts into the small row of a 15-20cm long, and the line-spacing of every row is 10cm；Control group is set simultaneously, often
1 part of perceptual rice check variety TN1 is inserted between 10-20 row rice material；Each planting bed seedbed can sow 200-250 parts
Material；
After planting：Normal water and fertilizer management is carried out to rice shoot；
(4) excessive to connect worm identification；
In the 3 leaf phase of rice shoot, carried out connecing worm identification with 1~2 age BPH nymph, every seedling connects worm 15-20 head；Seedbed keeps opening
Formula does not cover fly net, is gently stirred with rod rice shoot 1-2 times daily, waterborne for degree insect is rushed to, so that insect is distributed
Uniformly；
(5) scientific management after connecing worm；
I seedbed is kept for no more the water layer of 1cm, in favor of the movable of worm sources and maintains conducive to the warm and humid of pest cause evil
Environment；
II, should pull on sunshade net when meeting sunburst daytime, reduce direct sunlight；
III, reduces indoor air flow flowing, when meeting obvious air-flow, is shutting authentication room windward side windshield window, is meeting strong
Wind weather will close whole glazed doors；
(6) result and evaluation of Resistance Identification；
The victimization state for investigating each part material for two days after feeling the plant death of worm check variety TN1 whole, referring to subscript
Standard defines the level to each seedling, finally calculates the average mined grade of each part material；
Resistance rating scale：
0 grade (0.0~0.9) is immune (I), no damage symptoms；
1 grade (1.0~1.9) is highly resistance (HR), aggrieved unobvious；
3 grades (2.0~3.9) are anti-(R), and the first and second leaf turns yellow；
5 grades (4.0~5.9) be in resist (MR), the first to three leaf turn yellow or plant present downgrade；
7 grades (6.0~7.9) are middle sense (MS), and plant starts wilting；
9 grades (8.0~9.0) are high sense (HS), and plant is dead；
(7) transplanting of resistant material；
Antagonism and segregating population 0 grade to 3 grades of material obtained are transplanted to crop field in 5 leaf phases, normal by crop field
Management and sowing.
As a preferred option of the technical scheme, step (2) insectary temperature control at 25-30 DEG C, and have light, temperature,
Wet adjusting and ventilation facility.
As a preferred option of the technical scheme, the raising of step (2) worm sources uses no disease and pests harm, without any worm sources, nothing
The TN1 Adult plant plant of pesticide residue makees feed.
As a preferred option of the technical scheme, the quantity of raising worm sources is in the step (2)：The 1 feeding worm sources of TN1 feed institute of section are used
In 10 parts of F3 for above rice material, the quantity that worm is used in identification is prepared with this.
As a preferred option of the technical scheme, the temperature when step (4) is identified is controlled at 20-34 DEG C.
As a preferred option of the technical scheme, the worm that connects of step (4) the BPH nymph needs to complete in three days.
As a preferred option of the technical scheme, planting bed is applied compound fertilizer by 20 kilograms of dosages per acre and is made before the step (3) is sowed
Base manure.
Method of the invention is applied on rice breeding, and the breeding speed of rice can be accelerated.
Beneficial effects of the present invention：
1, the present invention establishes " the anti-accurate identification method of brown plant-hopper scale of rice seedling ", is the breeding of rice brown planthopper resistant
Large-scale breeding progeny Resistance Identification provides efficient technical method.The resistance breeding material of general large, medium and small various scales
Material, can disposably identify and finish, qualification result is accurate, to effectively facilitate the progress of resistance breeding project.
2, the present invention is suitable for the mapping population of genetic analysis research and the genetic marker positioning of Bph genes
Building, especially when conventional identification method is not applied for large-scale mapping population building and precisely analysis, the present invention
Can accurate identification technology more effectively be provided for the building and pedigree analysis of extensive mapping population.
3, in rice brown planthopper resistant breeding process, when resistant gene source is unclear, genetic background is complicated, resistance trait
When the molecular labeling not being applicable in, or when marker assisted selection is difficult to precise Identification resistant plant, the present invention provides one kind
Effective resistant plant screens alternative.By detecting breeding material, that is, can determine whether to be had by sample material brown
Plant hopper resistance, and then pest-resistant cultivar or strain are quickly screened for rice breeding.The present invention have it is easy to detect quickly, facilitate
The breeding cycle for shortening rice varieties, effectively improves breeding efficiency.
4, the present invention can touch enough numbers using the excessive identification for connecing worm method and carrying out resistance to rice shoot, every plant of rice shoot
The pest of amount, it is ensured that the rice shoot of resistance all can be not dead, improves the accuracy rate of identification, it can be ensured that filtering out has good resist
The rice shoot of property.
5, the rice shoot after present invention docking worm carries out the management of science, guarantees to identify the growth that indoor environment is suitable for worm sources
And activity, the cause evil property for improving brown paddy plant hopper guarantee that rice shoot has enough brown planthopper resistant abilities to improve the standard of screening.
6,2000 parts or more of material can be disposably identified using identification method of the invention, scale is big, identification is accurate,
It is high-efficient, highly reliable.In many years operating process of the method for the present invention, there is the breeding progeny of known resistant gene to parent
The partial resistance material identified is detected, it is found that these resistant materials have resistant gene, the standard of qualification result
True rate can reach 100%.
Detailed description of the invention
Fig. 1 is one jiao of large-scale authentication room of the invention and identifies preceding seedling growth situation map, and diagram authentication room is divided into multiple
Planting bed, each planting bed seedbed can sow 200 parts of materials, and the seedling of planting bed can be used for connecing worm identification.
Fig. 2 is that the present invention connects part Resistance Identification fructufy figure after worm identification, illustrates the water that each every part of pond of identification is identified
The anti-perception difference of rice test material is obvious.0 grade of resistant material is not aggrieved, 1 grade of resistant material is not caused harm substantially, 9 grades of perceptual materials
Expect that whole plant are dead.Full figure from left to right, from top to bottom totally 10 figure, respectively Fig. 2A~Fig. 2 J.Fig. 2A is from left to right each
The average resistance rank of part expert evidence (every part of 1 row, similarly hereinafter) is respectively 9 grades, 9 grades, 9 grades, 9 grades, 5 grades, 3 grades, 3 grades, 5 grades, 7
Grade；Fig. 2 B from left to right each part expert evidence be averaged resistance rank be respectively 3 grades, 3 grades, 1 grade, 1 grade, 1 grade, 5 grades, 5 grades, 9 grades,
9 grades, 9 grades, 9 grades, 9 grades；From left to right each part expert evidence resistance rank that is averaged is respectively 1 grade, 9 grades, 9 grades, 9 grades, 9 to Fig. 2 C
Grade, 7 grades, 7 grades, 5 grades, 3 grades, 5 grades, 3 grades, 5 grades, 5 grades；From left to right each part expert evidence resistance rank that is averaged is respectively Fig. 2 D
9 grades, 7 grades, 9 grades, 9 grades, 9 grades, 9 grades, 9 grades, 1 grade, 9 grades, 9 grades, 9 grades, 9 grades, 9 grades, 9 grades；Fig. 2 E from left to right each part identification
The material resistance rank that is averaged is respectively 9 grades, 9 grades, 9 grades, 9 grades, 9 grades, 9 grades, 9 grades, 0 grade, 1 grade, 0 grade, 1 grade, 5 grades, 7 grades, 9
Grade, 9 grades, 9 grades；Fig. 2 F from left to right each part expert evidence be averaged resistance rank be respectively 9 grades, 7 grades, 7 grades, 7 grades, 1 grade, 5 grades,
7 grades, 3 grades, 1 grade, 3 grades, 3 grades, 7 grades of grades；From left to right each part expert evidence resistance rank that is averaged is respectively 9 grades, 9 grades, 9 to Fig. 2 G
Grade, 7 grades, 5 grades, 3 grades, 3 grades, 3 grades, 9 grades, 9 grades, 9 grades, 3 grades；From left to right each part expert evidence is averaged resistance rank point Fig. 2 H
It Wei not be 3 grades, 9 grades, 9 grades, 9 grades, 1 grade, 9 grades, 9 grades, 1 grade, 5 grades, 9 grades, 9 grades；It is 1 grade, 3 that Fig. 2 I, which has 4 parts of expert evidence resistances,
Part material resistance is 3 grades, remaining material resistance is 9 grades；From left to right each part expert evidence resistance rank that is averaged is respectively 3 to Fig. 2 J
Grade, 0 grade, 9 grades, 9 grades, 5 grades, 1 grade, 9 grades, 9 grades, 3,9 grades, 9 grades, 3 grades.
Specific embodiment
The present invention is further illustrated below by specific embodiment, so that advantages and features of the invention are easier to be managed
Solution, it should be understood that following embodiment is only preferred version of the invention, rather than limiting the invention.
Embodiment 1
A kind of method that the anti-brown plant-hopper scale of rice seedling is precisely identified, includes the following steps：
(1) building of large-scale authentication room, large-scale authentication room include glass caping, sunshade net, stainless (steel) wire, fly net, push away
Sliding door and window, planting bed, automatic irrigation system；Stainless (steel) wire is located at the surrounding of authentication room, is equipped with insect prevention in the inside of stainless (steel) wire
Net forms double shielding；The surrounding of authentication room is provided with sliding door and window to be opened/closed；Sunshade net is arranged under glass caping
Side, sunshade net light transmittance are 20%, and sunshade net movable can be opened and closed；Planting bed is uniformly neatly arranged in authentication room, plantation
A length of 2.4 meters of pond, width are 1.2 meters, and depth is 0.5 meter；Husky loamy texture rice soil is filled in it；Automatic irrigation system and each planting bed
It is connected；The area of authentication room is 100 square metres, is highly 3 meters.
(2) worm is used in raising identification；
Brown plant-hopper worm sources select local field worm sources, at the beginning of early season worm sources annual 3 months~4 months in field or the wild rice of passing the winter
Field capture, late season worm sources are then captured in mid-August from field；The worm sources of capture supporting with the sealing with fly net in insectary
The raising of worm cage；The egg-laying season adult that puberty neat and consistent is chosen in next-generation worm carries out mass propagation, obtains for 1~2 age
Nymph connects worm Resistance Identification for rice；The temperature of insectary is controlled at 25 DEG C, and has light, temperature, wet adjusting and ventilation
Facility；The raising of worm sources makees feed using no disease and pests harm, without the TN1 Adult plant plant of any worm sources, non agricultural chemical residuum；Raising
The quantity of worm sources is：1 section's TN1 feed feeding worm sources for 10 parts of F3 for above rice material, identification is prepared with worm with this
Quantity.
(3) extensive rice growing；
Prepare before sowing：10-15 days before sowing, carried out disinfection with insecticide to planting bed and authentication room space；Planting bed
It poured water, harrow rotten, making ridge into smooth sowing seedbed, surrounding stays ditch to facilitate and irrigate；Planting bed is applied by 20 kilograms of dosages per acre
Compound fertilizer makees base manure.
Sowing：The rice material of every part of F3 or more generation broadcasts 40 vernalization seeds, and F2 broadcasts 500 or more for separation material
Seed；Every part of material broadcasts into the small row of a 15cm long, and the line-spacing of every row is 10cm；Control group is set simultaneously, every 10 rows
1 part of perceptual rice check variety TN1 is inserted between rice material；Each planting bed seedbed can sow 200 parts of materials.
After planting：Normal water and fertilizer management is carried out to rice shoot.
(4) excessive to connect worm identification；
Temperature when identification is controlled at 20 DEG C；In the 3 leaf phase of rice shoot, carried out connecing worm identification with 1~2 age BPH nymph, every seedling connects
Worm 15；The worm that connects of BPH nymph needs to complete in three days；Seedbed keeps open, does not cover fly net, light with rod daily
Gently stir rice shoot 1 time, it is waterborne for degree insect is rushed to, so that insect is evenly distributed.
(5) scientific management after connecing worm；
I seedbed is kept for no more the water layer of 1cm, in favor of the movable of worm sources and maintains conducive to the warm and humid of pest cause evil
Environment；
II, should pull on sunshade net when meeting sunburst daytime, reduce direct sunlight；
III, reduces indoor air flow flowing, when meeting obvious air-flow, is shutting authentication room windward side windshield window, is meeting strong
Wind weather will close whole glazed doors.
(6) result and evaluation of Resistance Identification；
The victimization state for investigating each part material for two days after feeling the plant death of worm check variety TN1 whole, referring to subscript
Standard defines the level to each seedling, finally calculates the average mined grade of each part material；
Resistance rating scale：
0 grade (0.0~0.9) is immune (I), no damage symptoms；
1 grade (1.0~1.9) is highly resistance (HR), aggrieved unobvious；
3 grades (2.0~3.9) are anti-(R), and the first and second leaf turns yellow；
5 grades (4.0~5.9) be in resist (MR), the first to three leaf turn yellow or plant present downgrade；
7 grades (6.0~7.9) are middle sense (MS), and plant starts wilting；
9 grades (8.0~9.0) are high sense (HS), and plant is dead；
(7) transplanting of resistant material；
Antagonism and segregating population 0 grade to 3 grades of material obtained are transplanted to crop field in 5 leaf phases, normal by crop field
Management and sowing.
Technical application verifying one：The technical method that is described using above-mentioned (1)-(6), to brown planthopper resistant major gene resistance
Paddy rice cross breeding breeding progeny carries out Resistance Identification and PCR detection gene verifying.Worm rice varieties TN1 will be felt and have BPH29's
Rice Resistance brown plant-hopper resource RBPH54 hybridization, offspring obtain 500 F5 familys from accompany each other generation and breeding, carry out to F5 family
Resistance Identification obtains 30 parts of resistance strains.With the molecular labeling BYL8 (primer sequence of close linkage：5'-
CCCACTTCCACAACCACA-3 ', 5 '-ATGCTCCTAGCTTCCTATTCC-3 ') detection, it is found that all familys all have
The specific amplified segment of 190bp shows that Resistance Identification result 100% is accurate.
Embodiment 2
A kind of method that the anti-brown plant-hopper scale of rice seedling is precisely identified, includes the following steps：
(1) building of large-scale authentication room, large-scale authentication room include glass caping, sunshade net, stainless (steel) wire, fly net, push away
Sliding door and window, planting bed, automatic irrigation system；Stainless (steel) wire is located at the surrounding of authentication room, is equipped with insect prevention in the inside of stainless (steel) wire
Net forms double shielding；The surrounding of authentication room is provided with sliding door and window to be opened/closed；Sunshade net is arranged under glass caping
Side, sunshade net light transmittance are 40%, and sunshade net movable can be opened and closed；Planting bed is uniformly neatly arranged in authentication room, plantation
A length of 3.0 meters of pond, width are 1.5 meters, and depth is 0.8 meter；Husky loamy texture rice soil is filled in it；Automatic irrigation system and each planting bed
It is connected；The area of authentication room is 200 square metres, is highly 5 meters.
(2) worm is used in raising identification；
Brown plant-hopper worm sources select local field worm sources, at the beginning of early season worm sources annual 3 months~4 months in field or the wild rice of passing the winter
Field capture, late worm sources mid-August in season capture from field；The worm sources of capture are in insectary with the feeding worm of the sealing with fly net
Cage raising；The egg-laying season adult that puberty neat and consistent is chosen in next-generation worm carries out mass propagation, if obtaining for 1~2 age
Worm connects worm Resistance Identification for rice；The temperature of insectary is controlled at 30 DEG C, and has light, temperature, wet adjusting and ventilation to set
It applies；The raising of worm sources makees feed using no disease and pests harm, without the TN1 Adult plant plant of any worm sources, non agricultural chemical residuum；Raise worm
The quantity in source is：1 section's TN1 feed feeding worm sources for 10 parts of F3 for above rice material, the number that worm is used in identification is prepared with this
Amount.
(3) extensive rice growing
Prepare before sowing：10-15 days before sowing, carried out disinfection with insecticide to planting bed and authentication room space；Planting bed
It poured water, harrow rotten, making ridge into smooth sowing seedbed, surrounding stays ditch to facilitate and irrigate；Planting bed is applied by 20 kilograms of dosages per acre
Compound fertilizer makees base manure.
Sowing：The rice material of every part of F3 or more generation broadcasts 60 vernalization seeds, and F2 broadcasts 500 or more for separation material
Seed；Every part of material broadcasts into the small row of a 20cm long, and the line-spacing of every row is 10cm；Control group is set simultaneously, every 20 rows
1 part of perceptual rice check variety TN1 is inserted between rice material；Each planting bed seedbed can sow 250 parts of materials.
After planting：Normal water and fertilizer management is carried out to rice shoot.
(4) excessive to connect worm identification；
Temperature when identification is controlled at 34 DEG C；In the 3 leaf phase of rice shoot, carried out connecing worm identification with 1~2 age BPH nymph, every seedling connects
Worm 20；The worm that connects of BPH nymph needs to complete in three days；Seedbed keeps open, does not cover fly net, light with rod daily
Gently stir rice shoot 2 times, it is waterborne for degree insect is rushed to, so that insect is evenly distributed.
(5) scientific management after connecing worm；
I seedbed is kept for no more the water layer of 1cm, in favor of the movable of worm sources and maintains conducive to the warm and humid of pest cause evil
Environment；
II, should pull on sunshade net when meeting sunburst daytime, reduce direct sunlight；
III, reduces indoor air flow flowing, when meeting obvious air-flow, is shutting authentication room windward side windshield window, is meeting strong
Wind weather will close whole glazed doors.
(6) result and evaluation of Resistance Identification；
The victimization state for investigating each part material for two days after feeling the plant death of worm check variety TN1 whole, referring to subscript
Standard defines the level to each seedling, finally calculates the average mined grade of each part material；
Resistance rating scale：
0 grade (0.0~0.9) is immune (I), no damage symptoms；
1 grade (1.0~1.9) is highly resistance (HR), aggrieved unobvious；
3 grades (2.0~3.9) are anti-(R), and the first and second leaf turns yellow；
5 grades (4.0~5.9) be in resist (MR), the first to three leaf turn yellow or plant present downgrade；
7 grades (6.0~7.9) are middle sense (MS), and plant starts wilting；
9 grades (8.0~9.0) are high sense (HS), and plant is dead.
(7) transplanting of resistant material；
Antagonism and segregating population 0 grade to 3 grades of material obtained are transplanted to crop field in 5 leaf phases, normal by crop field
Management and sowing.
Technical application verifying two：The technical method that is described using above-mentioned (1)-(6), to brown planthopper resistant major gene resistance
Paddy rice cross breeding breeding progeny carries out Resistance Identification and PCR detection gene verifying.Worm rice varieties 9311 will be felt and have qBph29
(t) Rice Resistance brown plant-hopper strain 08BPH327 hybridizes, and offspring obtains 500 F5 familys, to F5 family from accompany each other generation and breeding
Resistance Identification is carried out, 102 parts of resistance strains are obtained.With the molecular labeling YM54 (primer sequence of close linkage：5'-
CTCTCCCCTCCAATCCTTTT-3 ', 5 '-AGCACCCTGGAAAGCAGTAG-3 ') detection, it is found that all familys all have
QBph29 (t) genetic fragment of 205bp shows that Resistance Identification result 100% is accurate.
Embodiment 3
A kind of method that the anti-brown plant-hopper scale of rice seedling is precisely identified, includes the following steps：
(1) large-scale authentication room, large-scale authentication room include glass caping, sunshade net, stainless (steel) wire, fly net, sliding door and window,
Planting bed, automatic irrigation system；Stainless (steel) wire is located at the surrounding of authentication room, is equipped with fly net in the inside of stainless (steel) wire, is formed
Double shielding；The surrounding of authentication room is provided with sliding door and window to be opened/closed；The lower section of glass caping, sunshade is arranged in sunshade net
Net light transmittance is 30%, and sunshade net movable can be opened and closed；Planting bed is uniformly neatly arranged in authentication room, and planting bed is a length of
2.6 meters, width is 1.4 meters, and depth is 0.6 meter；Husky loamy texture rice soil is filled in it；Automatic irrigation system is connected with each planting bed
It connects；The area of authentication room is 150 square metres, is highly 4 meters.
(2) worm is used in raising identification；
Brown plant-hopper worm sources select local field worm sources, at the beginning of early season worm sources annual 3 months~4 months in field or the wild rice of passing the winter
Field capture, late worm sources mid-August in season capture from field；The worm sources of capture are in insectary with the feeding worm of the sealing with fly net
Cage raising；The egg-laying season adult that puberty neat and consistent is chosen in next-generation worm carries out mass propagation, if obtaining for 1~2 age
Worm connects worm Resistance Identification for rice；The temperature of insectary is controlled at 27 DEG C, and has light, temperature, wet adjusting and ventilation to set
It applies；The raising of worm sources makees feed using no disease and pests harm, without the TN1 Adult plant plant of any worm sources, non agricultural chemical residuum；Raise worm
The quantity in source is：1 section's TN1 feed feeding worm sources for 10 parts of F3 for above rice material, the number that worm is used in identification is prepared with this
Amount.
(3) extensive rice growing；
Prepare before sowing：First 13 days of sowing, carries out disinfection to planting bed and authentication room space with insecticide；Planting bed is through filling
Water harrows rotten, making ridge into smooth sowing seedbed, and surrounding stays ditch to facilitate and irrigate；Planting bed is applied compound by 20 kilograms of dosages per acre
Fertilizer makees base manure.
Sowing：The rice material of every part of F3 or more generation broadcasts 50 vernalization seeds, and F2 broadcasts 500 or more for separation material
Seed；Every part of material broadcasts into the small row of a 17cm long, and the line-spacing of every row is 10cm；Control group is set simultaneously, every 15 rows
1 part of perceptual rice check variety TN1 is inserted between rice material；Each planting bed seedbed can sow 225 parts of materials.
After planting：Normal water and fertilizer management is carried out to rice shoot.
(4) excessive to connect worm identification；
Temperature when identification is controlled at 27 DEG C；In the 3 leaf phase of rice shoot, carried out connecing worm identification with 1~2 age BPH nymph, every seedling connects
Worm 17；The worm that connects of BPH nymph needs to complete in three days；Seedbed keeps open, does not cover fly net, light with rod daily
Gently stir rice shoot 1 time, it is waterborne for degree insect is rushed to, so that insect is evenly distributed.
(5) scientific management after connecing worm；
I seedbed is kept for no more the water layer of 1cm, in favor of the movable of worm sources and maintains conducive to the warm and humid of pest cause evil
Environment；
II, should pull on sunshade net when meeting sunburst daytime, reduce direct sunlight；
III, reduces indoor air flow flowing, when meeting obvious air-flow, is shutting authentication room windward side windshield window, is meeting strong
Wind weather will close whole glazed doors；
(6) result and evaluation of Resistance Identification；
The victimization state for investigating each part material for two days after feeling the plant death of worm check variety TN1 whole, referring to subscript
Standard defines the level to each seedling, finally calculates the average mined grade of each part material；
Resistance rating scale：
0 grade (0.0~0.9) is immune (I), no damage symptoms；
1 grade (1.0~1.9) is highly resistance (HR), aggrieved unobvious；
3 grades (2.0~3.9) are anti-(R), and the first and second leaf turns yellow；
5 grades (4.0~5.9) be in resist (MR), the first to three leaf turn yellow or plant present downgrade；
7 grades (6.0~7.9) are middle sense (MS), and plant starts wilting；
9 grades (8.0~9.0) are high sense (HS), and plant is dead；
(7) transplanting of resistant material；
Antagonism and segregating population 0 grade to 3 grades of material obtained are transplanted to crop field in 5 leaf phases, normal by crop field
Management and sowing.
Technical application verifying three：The technical method that is described using above-mentioned (1)-(5), to brown planthopper resistant major gene resistance
Paddy rice cross breeding breeding progeny carries out Resistance Identification and PCR detection gene verifying.Rice Resistance source 05BPH16 [contains resistant gene
QBph30 (t)] hybridized with 9311, Resistance Identification is carried out to Offspring F2 segregating generation, 1000 seedling stage plant is identified altogether, has
742 plants of resistance seedling, there is 258 plants of perceptual seedling, segregation ratio meets 3：1 ratio (X2=0.341).Utilize brown planthopper resistant major gene resistance
QBph30 (t) specific molecular mark QY14 (primer sequence 5 '-AGCCCAAATGGAACAAACAA-3 ', 5 '-
GCTCACGGTTAAGCAATGGT-3 ') PCR detection is carried out to 258 resistant plants identified, find the equal energy of all familys
The genetic fragment of the 247bp of amplifying rice brown planthopper resistant major gene resistance qBph30 (t) shows that Resistance Identification result accuracy rate reaches
100%, while also indicating that the anti-accurate identification method of brown plant-hopper scale of this rice seedling is suitable for the heredity point of resistant gene
The building of analysis research and mapping population.
Embodiment 4
BPHR96 and Ptb33 is highly resistance brown plant-hopper resource, has great application value, but its to rice resistance breeding
There are no finely positionings for resistant gene, have bigger difficulty with marker assisted selection breeding.To utilize the two resistances as early as possible
Resource Cultivation goes out resistant variety, therefore is carried out using " the anti-accurate identification method of brown plant-hopper scale of rice seedling " of the invention
Rice breeding work.
1, rice resistance crossbreeding lines progeny is established
Pest-resistant resource BPHR96 and Ptb33 are hybridized, filial generation its F1 again with the Elite restorer line R155 of sense worm,
R1238, holding are that 209B reestablishes diplomatic relations respectively, and the offspring that reestablishes diplomatic relations is that 209B is returned 1 time with former restorer R155 and R1238, holding again, after
In per generation since BC1 in generation, screens fine individual plant, and plantation, selfing and selection obtain the BC4 strain kind of each cross combination to BC3 generation
Son.Its BPHR96/Ptb33//R155//55 parts of breeding strain of acquisition of/R155 cross combination selection, BPHR96/Ptb33//
R1238/ //R1238 cross combination selection obtains 80 parts of breeding strain, BPHR96/Ptb33//209B/ // 209B cross combination choosing
Select 60 parts of breeding strain of acquisition.
2, rice brown planthopper resistant Preliminary Identification
Screening and identification is carried out to three above-mentioned cross combinations using following methods.
(1) large-scale authentication room, large-scale authentication room include glass caping, sunshade net, stainless (steel) wire, fly net, sliding door and window,
Planting bed, automatic irrigation system；Stainless (steel) wire is located at the surrounding of authentication room, is equipped with fly net in the inside of stainless (steel) wire, is formed
Double shielding；The surrounding of authentication room is provided with sliding door and window to be opened/closed；The lower section of glass caping, sunshade is arranged in sunshade net
Net light transmittance is 30%, and sunshade net movable can be opened and closed；Planting bed is uniformly neatly arranged in authentication room, and planting bed is a length of
2.8 meters, width is 1.4 meters, and depth is 0.5 meter；Husky loamy texture rice soil is filled in it；Automatic irrigation system is connected with each planting bed
It connects；The area of authentication room is 180 square metres, is highly 5 meters；
(2) worm is used in raising identification；
Brown plant-hopper worm sources select local field worm sources, at the beginning of early season worm sources annual 3 months~4 months in field or the wild rice of passing the winter
Field capture, late worm sources mid-August in season capture from field；The worm sources of capture are in insectary with the feeding worm of the sealing with fly net
Cage raising；The egg-laying season adult that puberty neat and consistent is chosen in next-generation worm carries out mass propagation, if obtaining for 1~2 age
Worm connects worm Resistance Identification for rice；The temperature of insectary is controlled at 25 DEG C, and has light, temperature, wet adjusting and ventilation to set
It applies；The raising of worm sources makees feed using no disease and pests harm, without the TN1 Adult plant plant of any worm sources, non agricultural chemical residuum；Raise worm
The quantity in source is：1 section's TN1 feed feeding worm sources for 10 parts of F3 for above rice material, the number that worm is used in identification is prepared with this
Amount；
(3) extensive rice growing；
Prepare before sowing：First 14 days of sowing, carries out disinfection to planting bed and authentication room space with insecticide；Planting bed is through filling
Water harrows rotten, making ridge into smooth sowing seedbed, and surrounding stays ditch to facilitate and irrigate；Planting bed is applied compound by 20 kilograms of dosages per acre
Fertilizer makees base manure；
Sowing：The rice material of every part of F3 or more generation broadcasts 55 vernalization seeds, and F2 broadcasts 500 or more for separation material
Seed；Every part of material broadcasts into the small row of a 18cm long, and the line-spacing of every row is 10cm；Control group is set simultaneously, every 18 rows
1 part of perceptual rice check variety TN1 is inserted between rice material；Each planting bed seedbed can sow 240 parts of materials；
After planting：Normal water and fertilizer management is carried out to rice shoot；
(4) excessive to connect worm identification；
Temperature when identification is controlled at 31 DEG C；In the 3 leaf phase of rice shoot, carried out connecing worm identification with 1~2 age BPH nymph, every seedling connects
Worm 19；The worm that connects of BPH nymph needs to complete in three days；Seedbed keeps open, does not cover fly net, light with rod daily
Gently stir rice shoot 2 times, it is waterborne for degree insect is rushed to, so that insect is evenly distributed；
(5) scientific management after connecing worm；
I seedbed is kept for no more the water layer of 1cm, in favor of the movable of worm sources and maintains conducive to the warm and humid of pest cause evil
Environment；
II, should pull on sunshade net when meeting sunburst daytime, reduce direct sunlight；
III, reduces indoor air flow flowing, when meeting obvious air-flow, is shutting authentication room windward side windshield window, is meeting strong
Wind weather will close whole glazed doors；
(6) result and evaluation of Resistance Identification；
The victimization state for investigating each part material for two days after feeling the plant death of worm check variety TN1 whole, referring to subscript
Standard defines the level to each seedling, finally calculates the average mined grade of each part material；
Resistance rating scale：
0 grade (0.0~0.9) is immune (I), no damage symptoms；
1 grade (1.0~1.9) is highly resistance (HR), aggrieved unobvious；
3 grades (2.0~3.9) are anti-(R), and the first and second leaf turns yellow；
5 grades (4.0~5.9) be in resist (MR), the first to three leaf turn yellow or plant present downgrade；
7 grades (6.0~7.9) are middle sense (MS), and plant starts wilting；
9 grades (8.0~9.0) are high sense (HS), and plant is dead；
(7) transplanting of resistant material；
Antagonism and segregating population 0 grade to 3 grades of material obtained are transplanted to crop field in 5 leaf phases, normal by crop field
Management and sowing.
Utilize technical method described in (1)-(6) to the 195 parts of resistance breeding materials screened from 3 cross combinations
Carry out seedling stage anti-brown plant-hopper identification.Qualification result：
BPHR96/Ptb33//R155/ //R155 cross combination obtains 1 grade of 8 parts of resistant strain, 6 parts of 3 grades,
BPHR96/Ptb33//R1238/ //R1238 cross combination obtains 1 grade of 12 parts of resistant strain, 14 parts of 3 grades,
BPHR96/Ptb33//209B/ // 209B cross combination obtains 1 grade of 8 parts of resistant strain, 8 parts of 3 grades.
3, rice brown planthopper resistant repetitive identified
Combine material plantation that brown planthopper resistant Preliminary Identifications obtain in crop field to above-mentioned 3, according to breeding character to its into
Row screening, selects 10 parts, 18 parts and 12 parts of merit strain respectively.This 40 parts of materials are described according to above-mentioned (1)-(6)
Technical method carries out seedling stage anti-brown plant-hopper repetitive identified, and as a result all material all shows as resistance, wherein
BPHR96/Ptb33//R155/ //1 grade of R155 cross combination resistance has 8 parts, 2 parts of 3 grades,
BPHR96/Ptb33//R1238/ //1 grade of R1238 cross combination resistance has 16 parts, 2 parts of 3 grades,
BPHR96/Ptb33//209B/ // 209B cross combination obtains 1 grade of 6 parts of resistant strain, 2 parts of 3 grades.
These materials move on to field planting, and restorer can be used for the screening of resistance Hybrid Rice Combinations after harvest seed, protect
Holding is transformation for resistance sterile line.
Claims (5)
1. a kind of method that the anti-brown plant-hopper scale of rice seedling is precisely identified, it is characterised in that：Include the following steps：
(1) building of large-scale authentication room, large-scale authentication room includes glass caping, sunshade net, stainless (steel) wire, fly net, sliding door
Window, planting bed, automatic irrigation system；Stainless (steel) wire is located at the surrounding of authentication room, is equipped with fly net, shape in the inside of stainless (steel) wire
At double shielding；The surrounding of authentication room is provided with sliding door and window to be opened/closed；The lower section of glass caping is arranged in sunshade net, hides
Screened postive light transmittance is 20-40%, and sunshade net movable can be opened and closed；Planting bed is uniformly neatly arranged in authentication room, planting bed
2.4-3.0 meters a length of, width is 1.2-1.5 meters, and depth is 0.5-0.8 meters；Husky loamy texture rice soil is filled in it；Automatic irrigation system with
Each planting bed is connected；The area of authentication room is 100-200 square metres, is highly 3-5 meters；
(2) worm is used in raising identification；
Brown plant-hopper worm sources select local field worm sources, at the beginning of early season worm sources annual 3 months~4 months in passing the winter field or wild rice field is caught
It obtains, late season worm sources are then captured in mid-August from field；The worm sources of capture are in insectary with the dependent insect cage of the sealing with fly net
Raising；The egg-laying season adult that puberty neat and consistent is chosen in next-generation worm carries out mass propagation, obtains the nymph in 1~2 age
Worm Resistance Identification is connect for rice；
(3) extensive rice growing；
Prepare before sowing：10-15 days before sowing, carried out disinfection with insecticide to planting bed and authentication room space；Planting bed is through filling
Water harrows rotten, making ridge into smooth sowing seedbed, and surrounding stays ditch to facilitate and irrigate；
Sowing：The rice material of every part of F3 or more generation broadcasts 40-60 vernalization seeds, and F2 broadcasts 500 or more for separation material
Seed；Every part of material broadcasts into the small row of a 15-20cm long, and the line-spacing of every row is 10cm；Control group is set simultaneously, every
1 part of perceptual rice check variety TN1 is inserted between 10-20 row rice material；Each planting bed seedbed can sow 200-250 parts of materials
Material；
After planting：Normal water and fertilizer management is carried out to rice shoot；
(4) excessive to connect worm identification；
In the 3 leaf phase of rice shoot, carried out connecing worm identification with 1~2 age BPH nymph, every seedling connects worm 15-20 head；Seedbed keeps open, no
Fly net is covered, is gently stirred with rod rice shoot 1-2 times daily, it is waterborne for degree insect is rushed to, so that insect is evenly distributed；
(5) scientific management after connecing worm；
I seedbed is kept for no more the water layer of 1cm, in favor of the movable of worm sources and maintains conducive to the warm and humid environment of pest cause evil；
II, should pull on sunshade net when meeting sunburst daytime, reduce direct sunlight；
III, reduces indoor air flow flowing, when meeting obvious air-flow, is shutting authentication room windward side windshield window, is meeting high wind day
Gas will close whole glazed doors；
(6) result and evaluation of Resistance Identification；
The victimization state for investigating each part material for two days after feeling the plant death of worm check variety TN1 whole, referring to following standard pair
Each seedling is defined the level, and the average mined grade of each part material is finally calculated；
Resistance rating scale：
0 grade (0.0~0.9) is immune (I), no damage symptoms；
1 grade (1.0~1.9) is highly resistance (HR), aggrieved unobvious；
3 grades (2.0~3.9) are anti-(R), and the first and second leaf turns yellow；
5 grades (4.0~5.9) be in resist (MR), the first to three leaf turn yellow or plant present downgrade；
7 grades (6.0~7.9) are middle sense (MS), and plant starts wilting；
9 grades (8.0~9.0) are high sense (HS), and plant is dead；
(7) transplanting of resistant material；
Antagonism and segregating population 0 grade to 3 grades of material obtained are transplanted to crop field in 5 leaf phases, by crop field normal management
And sowing.
2. the method that the anti-brown plant-hopper scale of rice seedling according to claim 1 is precisely identified, it is characterised in that：Institute
The temperature for stating step (2) insectary is controlled at 25-30 DEG C, and has light, temperature, wet adjusting and ventilation facility.
3. the method that the anti-brown plant-hopper scale of rice seedling according to claim 1 is precisely identified, it is characterised in that：Institute
Temperature when stating step (4) identification is controlled at 20-34 DEG C.
4. the method that the anti-brown plant-hopper scale of rice seedling according to claim 1 is precisely identified, it is characterised in that：Institute
The worm that connects for stating step (4) BPH nymph needs to complete in three days.
5. the application for the method that the anti-brown plant-hopper scale of rice seedling as described in requiring 1 is precisely identified, it is characterised in that：It should
Application of the method on rice breeding.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110476750A (en) * | 2019-08-23 | 2019-11-22 | 湖南隆平高科种业科学研究院有限公司 | A kind of rice seedling brown planthopper resistant scale identification and screening technique |
| CN111264320A (en) * | 2020-03-06 | 2020-06-12 | 湖南杂交水稻研究中心 | Method for accurately identifying resistance of brown planthopper in adult stage of rice |
| CN114271158A (en) * | 2021-12-30 | 2022-04-05 | 华智生物技术有限公司 | Method for identifying resistance of brown planthopper of rice in field adult stage |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101822187A (en) * | 2010-04-30 | 2010-09-08 | 浙江省农业科学院 | Method for evaluating rice planthopper-resistant property of rice |
| CN104521592A (en) * | 2015-01-04 | 2015-04-22 | 连云港市农业科学院 | Large-scale quantitative and strengthened inoculation authenticating method for paddy black-streaked dwarf disease resistance |
| CN105165502A (en) * | 2015-07-21 | 2015-12-23 | 中国水稻研究所 | Method for evaluating resistance of rice in adult-plant stage against sogatella furcifera under semi-field conditions |
-
2018
- 2018-06-12 CN CN201810598811.4A patent/CN108812125A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101822187A (en) * | 2010-04-30 | 2010-09-08 | 浙江省农业科学院 | Method for evaluating rice planthopper-resistant property of rice |
| CN104521592A (en) * | 2015-01-04 | 2015-04-22 | 连云港市农业科学院 | Large-scale quantitative and strengthened inoculation authenticating method for paddy black-streaked dwarf disease resistance |
| CN105165502A (en) * | 2015-07-21 | 2015-12-23 | 中国水稻研究所 | Method for evaluating resistance of rice in adult-plant stage against sogatella furcifera under semi-field conditions |
Non-Patent Citations (2)
| Title |
|---|
| 江西省质量技术监督局: "DB36-T952—2017水稻对褐飞虱抗性鉴定技术规程", 《江西省地方标准》 * |
| 王传之: "利用MAS聚合水稻基因Xa23和bph20(t)选育抗病虫恢复系的研究", 《万方学位论文》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110476750A (en) * | 2019-08-23 | 2019-11-22 | 湖南隆平高科种业科学研究院有限公司 | A kind of rice seedling brown planthopper resistant scale identification and screening technique |
| CN110476750B (en) * | 2019-08-23 | 2021-09-21 | 湖南隆平高科种业科学研究院有限公司 | Large-scale identification and screening method for brown planthopper resistance of rice in seedling stage |
| CN111264320A (en) * | 2020-03-06 | 2020-06-12 | 湖南杂交水稻研究中心 | Method for accurately identifying resistance of brown planthopper in adult stage of rice |
| CN114271158A (en) * | 2021-12-30 | 2022-04-05 | 华智生物技术有限公司 | Method for identifying resistance of brown planthopper of rice in field adult stage |
| CN114271158B (en) * | 2021-12-30 | 2022-12-23 | 华智生物技术有限公司 | Method for identifying resistance of brown planthopper of rice in field adult stage |
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Application publication date: 20181116 |