CN105165502A - Method for evaluating resistance of rice in adult-plant stage against sogatella furcifera under semi-field conditions - Google Patents
Method for evaluating resistance of rice in adult-plant stage against sogatella furcifera under semi-field conditions Download PDFInfo
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- CN105165502A CN105165502A CN201510430004.8A CN201510430004A CN105165502A CN 105165502 A CN105165502 A CN 105165502A CN 201510430004 A CN201510430004 A CN 201510430004A CN 105165502 A CN105165502 A CN 105165502A
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- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 92
- 235000009566 rice Nutrition 0.000 title claims abstract description 92
- 241000176086 Sogatella furcifera Species 0.000 title claims abstract description 32
- 238000000034 method Methods 0.000 title claims abstract description 27
- 240000007594 Oryza sativa Species 0.000 title 1
- 241000209094 Oryza Species 0.000 claims abstract description 91
- 241000196324 Embryophyta Species 0.000 claims abstract description 47
- 238000011156 evaluation Methods 0.000 claims abstract description 27
- 239000004568 cement Substances 0.000 claims abstract description 24
- 241001498622 Cixius wagneri Species 0.000 claims abstract description 19
- 230000006378 damage Effects 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 7
- 239000003337 fertilizer Substances 0.000 claims abstract description 5
- 244000179684 Passiflora quadrangularis Species 0.000 claims description 33
- 235000011266 Passiflora quadrangularis Nutrition 0.000 claims description 33
- 241000607479 Yersinia pestis Species 0.000 claims description 17
- 239000004677 Nylon Substances 0.000 claims description 13
- 229920001778 nylon Polymers 0.000 claims description 13
- 238000012512 characterization method Methods 0.000 claims description 7
- 238000010899 nucleation Methods 0.000 claims description 5
- 230000005070 ripening Effects 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims description 2
- 235000019994 cava Nutrition 0.000 claims 1
- 241000238631 Hexapoda Species 0.000 abstract description 3
- 230000007547 defect Effects 0.000 abstract 1
- 238000012797 qualification Methods 0.000 description 9
- 206010061217 Infestation Diseases 0.000 description 5
- 238000003556 assay Methods 0.000 description 4
- 241001556089 Nilaparvata lugens Species 0.000 description 3
- 230000007812 deficiency Effects 0.000 description 3
- 238000009331 sowing Methods 0.000 description 3
- 241001466044 Delphacidae Species 0.000 description 2
- 241000258937 Hemiptera Species 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 2
- 239000002917 insecticide Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 238000002791 soaking Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000500891 Insecta Species 0.000 description 1
- 241001470017 Laodelphax striatella Species 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 230000000692 anti-sense effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 235000021329 brown rice Nutrition 0.000 description 1
- 230000009194 climbing Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 231100000225 lethality Toxicity 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
- A01G22/22—Rice
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Botany (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Catching Or Destruction (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a method for evaluating resistance of rice in the adult-plant stage against sogatella furcifera under semi-field conditions. The method comprises: planting rice in a cement pond, wherein the rice comprises to-be-tested rice varieties, an insect-resistant check variety and a susceptible check variety; planting the susceptible check variety around the to-be-tested rice varieties and the insect-resistant check variety, and carrying out normal water and fertilizer management; in an initial tillering stage of rice, inoculating each tiller with a pair of newly emerged adults of sogatella furcifera, and allowing the sogatella furcifera to perform natural propagation; and according to the sogatella furcifera resistance identification and evaluation criteria for rice varieties in the adult-plant stage, when the damage of the susceptible check variety reaches level 7, starting evaluating resistance of the to-be-tested rice varieties until all the plants of the susceptible check variety are dead; wherein when all the plants of the susceptible check variety are dead, the resistance of the to-be-tested rice varieties is the final resistance. The method disclosed by the invention overcomes the problem in the prior art that rice planthopper resistance of massive rice varieties in the adult-plant stage cannot be identified in bulk, so that the defect that evaluation on resistance against sogatella furcifera can only be identified in a seedling period is remedied.
Description
(1) technical field
The present invention relates to a kind of Rice Resistance white-backed planthopper evaluating characteristics, particularly relate under controlled condition, by the Resistance Identification of paddy rice Adult plant Whitebacked Planthopper, improve Rice Resistance white-backed planthopper authenticate technology level.
(2) background technology
Rice fulgorid is Insecta, Homoptera (Homoptera), Delphacidae (Delphacidae) insect.To sting crops such as inhaling plant juice hazard rice, it is the primary pest in Rice Production.Frequent species has brown planthopper (Nilaparvatalugens), white-backed planthopper (Sogatellafurcifera) and small brown rice planthopper (Laodelphaxstriatellus).Rice fulgorid resides in rice stem base portion or fringe portion usually, main draws paddy rice juice, early stage at paddy growth, cause paddy growth slowly, delay of tillering; At Rise's boot period, when endangering serious, performance is not eared; At Grain Filling, then cause thousand kernel weight to alleviate, rate of immatured grain increases, Severe Reduction or withered.Except directly endangering, rice fulgorid, with the virus such as stripe virus disease, black streak dwarf, causes the popular of rice disease.The control of rice fulgorid mainly relies on efficient chemical insecticide, and Long-Time Service chemical insecticide can cause the rampant again of plant hopper and pesticide resistance, causes pesticide residues in rice simultaneously, thus affects rice quality.Selection utilization has the rice varieties of stronger resistance to be the most safe and effective method of control plant hopper to rice fulgorid.Domestic at present have seedling stage assay method and Adult plant field test method in Resistance of Rice Varieties evaluation.For continuing to use the standard group in seedling stage screening method (SSST method) founded International Rice Research Institute or the standard seedling screening method (MSST method) improved through Chinese scholar seedling stage always, namely evaluate in the resistance of rice seedling to rice fulgorid, using the resistance level in seedling stage as paddy rice to rice fulgorid Resistant index Instructing manufacture.The test of this group in recent years shows, a lot of rice varieties seedling stage and the resistance level of Adult plant to rice fulgorid inconsistent, some even has very large difference.Some kinds are bad at wheat seeding, but may increase in the resistance of Adult plant, also right on the contrary.Recent year scholar has invented with paddy rice Adult plant as qualification target, only with rice varieties lethality for evaluation criterion carries out against-plant hopper authentication method, compensate for the deficiency of only carrying out against-plant hopper evaluation with seedling stage assay, but, this kind of authentication method is adopted for rice varieties qualification in enormous quantities, needs the rice fulgorid of q.s not easily.Given this, in order to batch rice varieties carries out the qualification of Adult plant against-plant hopper, we have invented a kind of half anti-white-backed planthopper evaluation criterion in field newly.
(3) summary of the invention
The object of the invention is to provide the evaluation method of the anti-whitebacked planthopper-resistance of a kind of half field paddy rice Adult plant, overcome the problem can not carrying out the qualification of Adult plant against-plant hopper in prior art to batch rice varieties, compensate for the deficiency of only carrying out anti-white-backed planthopper evaluation with seedling stage assay.
The technical solution used in the present invention is:
The invention provides a kind of method of half field evaluation paddy rice Adult plant whitebacked planthopper-resistance, described method is: (1) sets up cement pit (described cement pit pond is along being provided with groove), and (described framework can be cuboid-type to build framework above cement pit, also can be shed type), described framework covers nylon wire screen covering, described nylon wire screen covering divides upper part grenadine and lower part grenadine, described upper part grenadine leaves extension, described upper part grenadine is provided with sample mouth, and described sample mouth is provided with the first snap close of movable connection; The bottom of described lower part grenadine is fixed on cement pit edge (being namely fixed in the groove on edge, cement pit pond), described lower part grenadine top is connected by movable the second snap close be connected with upper part grenadine, and described extension covers lower part grenadine outer surface (preferred extension grenadine height is 1/2 ~ 1 of lower part grenadine height); (2) rice cultivation in cement pit, described paddy rice comprises rice varieties to be measured, pest-resistant check variety and sense worm check variety, (rice varieties to be measured and pest-resistant check variety are planted at random for described rice varieties to be measured and pest-resistant check variety surrounding plantation sense worm check variety, repeat 3 times, often repeat plantation 3 cave), normal water and fertilizer management; Described sense worm check variety is TN1; Described pest-resistant check variety is IR56; (3) rice tillering initial stage (every cave stays 1 ~ 2 stalwartness to tiller), connect white-backed planthopper just emerging adult 1 is right often to tiller, natural propagation; (4) according to rice varieties Adult plant anti-white-backed planthopper characterization and evaluation standard, the resistance of rice varieties to be measured is started to evaluate when feeling worm check variety and reaching 7 grades, until sense worm check variety is all dead, when sense worm check variety is all dead, the resistance of kind to be measured is final resistance;
Described rice varieties Adult plant anti-white-backed planthopper characterization and evaluation standard is:
0 grade: plant is normal, without plant hopper Damage pattern; Resistance level is high resistance;
1 grade: plant and solid normal, have slight Damage pattern, base portion is without obvious blackout; Resistance level is pest-resistant;
3 grades: plant is downgraded not obvious, and ripening rate is low; Bottom leaf sheath blackout, has obvious plant hopper Damage pattern; Resistance level is anti-in being;
5 grades: plant is downgraded to some extent or turns to be yellow, ear normal, but diminish; Middle and lower part leaf sheath blackout; Resistance level is middle sense;
7 grades: plant is obviously downgraded, heading is postponed, and fringe is obviously little; Middle and lower part blade blackout, withered; Resistance level is sense worm;
9 grades: plant is closely withered or do not ear; Complete stool major part blade blackout; Resistance level is high sense.
Long 8 meters of cement pit of the present invention, wide 1.7 meters, high 0.8 meter, build high 1.5 meters of steelframe frames along cement pit surrounding, described steelframe frame has covered 40 order nylon wire screen coverings.
Rice varieties to be measured of the present invention and pest-resistant check variety, sense worm check variety each plantation 3 cave, repeat 3 times, every cave Hybrid Rice Varieties 1 ~ 2, conventional rice variety 2 ~ 3, seeding row spacing 15 × 20cm.
Nylon wire screen covering upper part grenadine of the present invention and lower part grenadine aspect ratio are 3:1 (preferred lower part grenadine 0.5 meter is high), and described sample mouth is perpendicular to cement pit edge.
Nylon wire screen covering upper part top of the present invention fills 1 sample mouth to bottom every 2 meter amperes, and described sample mouth extends to extension bottom (described sample mouth length is 3/4 ~ 1 of steelframe height).Described first snap close is slide fastener, described second snap close velcro.
The method of half field evaluation paddy rice Adult plant whitebacked planthopper-resistance of the present invention repeats when evaluating and testing rice varieties, takes the mean as resistance class, and grade determined by concrete reference table 2:
On average to be injured between table 2 repetition grade
Compared with prior art, beneficial effect of the present invention is mainly reflected in: the evaluation method that the invention provides a kind of half field paddy rice Adult plant whitebacked planthopper-resistance, the framework being coated with nylon wire upper cover is built in cement pit surrounding, and nylon wire screen covering divides upper and lower two parts, lower part is fixed, and sample mouth is set up on top separately, conveniently looks into worm, avoid simultaneously and avoid white-backed planthopper to fly out, improve the reliability of inspection; The present invention overcomes the problem can not carrying out the qualification of Adult plant against-plant hopper in prior art to batch rice varieties, compensate for only with the deficiency that the anti-white-backed planthopper of seedling stage assay is evaluated.
(4) accompanying drawing explanation
Fig. 1 is nylon wire screen covering front schematic view, 1-lower part grenadine, 2-upper part grenadine, 3-second snap close, 4-sample mouth, 5-upper part extension bottom, 6-upper part grenadine extension.
Fig. 2 is the half field photo that the present invention identifies for whitebacked planthopper-resistance.
Fig. 3 is half side, the field photo that the present invention identifies for whitebacked planthopper-resistance.
(5) embodiment
Below in conjunction with specific embodiment, the present invention is described further, but protection scope of the present invention is not limited in this:
The qualification of rice varieties Adult plant Whitebacked Planthopper resistance under embodiment 1: half field condition
1) with reference to Fig. 1-Fig. 3, set up cement pit, cement pit pond along being provided with groove, long 8 meters of cement pit, wide 1.7 meters, high 0.8 meter, the added high 1.5 meters of steelframe frames of cement pit, and in steelframe frame upper cover 40 order nylon wire screen coverings.Guard divides upper part and lower part, and upper part leaves extension, and upper part is high 1.3 meters, and extension is high 0.2 meter; Lower part grenadine is high about 0.5 meter, and the bottom compacting of lower part grenadine, in the groove at cement pit edge, prevents rice fulgorid from climbing out of; Lower part grenadine top is connected with upper part grenadine by velcro, and the extension of upper part grenadine covers lower part grenadine outer surface; Install 1 meter of long slide fastener along upper part top to direction, extension bottom, slide fastener and cement pit pond, along vertical, also can be with the consistent slide fastener of steelframe frame height, lower part be covered completely, fill one, conveniently look into worm at interval of 2 meter amperes.
2) sowing adopts some broadcast mode (also can adopt transplanting method, the operation such as seed soaking, vernalization, sowing, transplanting is carried out all routinely).Rice cultivation kind in pond, comprise rice varieties to be measured (11), pest-resistant check variety (IR56), sense worm check variety (TN1), rice varieties to be measured and pest-resistant check variety are planted at random, at kind to be measured, anti-sense worm check variety periphery plantation sense worm check variety, every kind repeats 3 times, repeats plantation 3 cave at every turn.Rice varieties to be measured and anti-, the every bunch planting kind of sense check variety 3 ~ 5 vernalization seed, seeding row spacing 15 × 20cm, normal water and fertilizer management.
3) treat that about the 10 days length of time rice seedlings grow rejected weak seedling, healthy seedling 1 ~ 2 (hybrid rice 1, conventional Rice 2) is stayed in every cave, and at the rice tillering initial stage, every cave stays 1 ~ 2 stalwartness to tiller, and connects first emerging adult 1 right, let alone breeding often to tiller.
4) according to rice varieties Adult plant anti-white-backed planthopper characterization and evaluation standard, when feeling worm check variety TN1 and reaching 7 grades, the resistance by rice varieties Adult plant anti-white-backed planthopper characterization and evaluation standard evaluation kind to be measured is started, until the contrast of sense worm is all dead.When the contrast of sense worm is all dead, the resistance of kind to be measured is final resistance.
Table 1 rice varieties Adult plant anti-white-backed planthopper characterization and evaluation standard
In triplicate, during grade of being on average injured between calculating repetition, process by table 2:
On average to be injured between table 2 repetition grade
Qualification result is obtained in table 3 according to above method, the kind 2 parts (river section 736, magnificent 3084S) resisted is identified during Adult plant Whitebacked Planthopper has altogether, middle sense kind 4 parts of (magnificent 1165S in 11 portions of paddy rice, spring excellent 84, Luo You 9348 and elegant water 134).
Table 3: the resistance of different rice varieties (being) Whitebacked Planthopper
| Variety name | Anti-level | Resistance level |
| River section 736 | 3 | MR |
| China 3084S | 3 | MR |
| China 1165S | 5 | MS |
| Spring excellent 84 | 5 | MS |
| Luo You 9348 | 5 | MS |
| Elegant water 134 | 5 | MS |
| Money 100B | 7 | S |
| Excellent No. 1 of Y two | 7 | S |
| Guangzhan 63S-4 | 7 | S |
| Excellent No. 1 of strain two | 9 | HS |
| 7011 | 9 | HS |
| IR56 | 1 | HR |
| TN1 | 9 | HS |
In embodiment 2: half field and field natural INFESTATION situation, near-isogenic rice lines compares
Under half field condition, the Resistance Identification of paddy rice Whitebacked Planthopper is with embodiment 1, and rice varieties to be measured (8) is in table 4.
The Resistance Identification of field natural INFESTATION Rice under Condition Whitebacked Planthopper:
(1) Quarter Design: average every 5.4 × 2 meters, community.The operation such as seed soaking, vernalization, sowing, transplanting is carried out all routinely, and 2 kinds to be measured are planted in every community, seeding row spacing 20 × 25cm.This transplanting of hybrid rice list, conventional Rice 2 ~ 3 transplanting.Each kind (comprising kind to be measured, pest-resistant contrast IR56) each 3 row, often row 27 cave, plant 1 row TN1 between two kinds to be measured or between kind to be measured and pest-resistant check variety, plant 2 row TN1 in kind to be measured and pest-resistant check variety periphery simultaneously.Each kind repeats 3 times, randomized arrangement.
(2) normal water and fertilizer management, in time feeling worm and contrast 7 grades, evaluate product to be tested kind, evaluation criterion is with embodiment 1.
(3) field natural INFESTATION, investigation finds that rice fulgorid is caused harm and to cause harm for comprehensive, and early stage, the later stage, based on brown planthopper, the results are shown in Table 4 based on white-backed planthopper.Result show according to authentication method of the present invention and criterion to the qualification result of Rice Resistance white-backed planthopper and field natural INFESTATION result basically identical, prove that the method qualification result is reliable.
Table 4: the resistance result of Rice Varieties plant hopper in half field Adult plant and natural INFESTATION situation
Claims (6)
1. a method for half field evaluation paddy rice Adult plant whitebacked planthopper-resistance, is characterized in that described method is: (1) sets up cement pit, and builds framework above cement pit, described framework is coated with nylon wire screen covering; Described nylon wire screen covering divides upper part grenadine and lower part grenadine, and described upper part grenadine leaves extension, and described upper part grenadine is provided with sample mouth, and described sample mouth is provided with the first snap close of movable connection; The bottom of described lower part grenadine is fixed on cement pit edge, and described lower part grenadine top is connected by movable the second snap close be connected with upper part grenadine, and described extension covers lower part grenadine outer surface; (2) rice cultivation in cement pit, described paddy rice comprises rice varieties to be measured, pest-resistant check variety and sense worm check variety, described rice varieties to be measured and pest-resistant check variety surrounding plantation sense worm check variety, normal water and fertilizer management; Described sense worm check variety is TN1; Described pest-resistant check variety is IR56; (3) at the rice tillering initial stage, connect white-backed planthopper just emerging adult 1 is right often to tiller, natural propagation; (4) according to rice varieties Adult plant anti-white-backed planthopper characterization and evaluation standard, the resistance of rice varieties to be measured is started to evaluate when feeling worm check variety and reaching 7 grades, until sense worm check variety is all dead, when sense worm check variety is all dead, the resistance of rice varieties to be measured is final resistance;
Described rice varieties Adult plant anti-white-backed planthopper characterization and evaluation standard is:
0 grade: plant is normal, without plant hopper Damage pattern; Resistance level is high resistance;
1 grade: plant and solid normal, have slight Damage pattern, base portion is without obvious blackout; Resistance level is pest-resistant;
3 grades: plant is downgraded not obvious, and ripening rate is low; Bottom leaf sheath blackout, has obvious plant hopper Damage pattern; Resistance level is anti-in being;
5 grades: plant is downgraded to some extent or turns to be yellow, ear normal, but diminish; Middle and lower part leaf sheath blackout; Resistance level is middle sense;
7 grades: plant is obviously downgraded, heading is postponed, and fringe is obviously little; Middle and lower part blade blackout, withered; Resistance level is sense worm;
9 grades: plant is closely withered or do not ear; Complete stool major part blade blackout; Resistance level is high sense.
2. the method for half field evaluation paddy rice Adult plant whitebacked planthopper-resistance as claimed in claim 1, it is characterized in that long 8 meters of described cement pit, wide 1.7 meters, high 0.8 meter, build high 1.5 meters of steelframe frames along cement pit surrounding, described steelframe frame has covered 40 order nylon wire screen coverings.
3. the method for half field evaluation paddy rice Adult plant whitebacked planthopper-resistance as claimed in claim 1, it is characterized in that described rice varieties to be measured and pest-resistant check variety, sense worm check variety plant 3 caves respectively, repeat 3 times, every cave Hybrid Rice Varieties 1 ~ 2, conventional rice variety 2 ~ 3, seeding row spacing 15 × 20cm.
4. the method for half field evaluation paddy rice Adult plant whitebacked planthopper-resistance as claimed in claim 2, it is characterized in that described nylon wire screen covering upper part grenadine and lower part grenadine aspect ratio are 3:1, described sample mouth is perpendicular to cement pit.
5. the method for half field evaluation paddy rice Adult plant whitebacked planthopper-resistance as claimed in claim 4, it is characterized in that described upper part grenadine top establishes 1 sample mouth to bottom every 2 meters, described sample mouth extends to extension bottom.
6. the method for half field evaluation paddy rice Adult plant whitebacked planthopper-resistance as claimed in claim 4, is characterized in that described first snap close is slide fastener, described second snap close velcro.
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Cited By (6)
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| CN108812125A (en) * | 2018-06-12 | 2018-11-16 | 广西大学 | A kind of anti-accurate identification method of brown plant-hopper scale of rice seedling |
| CN111264320A (en) * | 2020-03-06 | 2020-06-12 | 湖南杂交水稻研究中心 | Method for accurately identifying resistance of brown planthopper in adult stage of rice |
| CN111557215A (en) * | 2020-02-27 | 2020-08-21 | 四川省农业科学院植物保护研究所 | Identification method and application of rice variety resisting plant hopper and SRBSDV (sequence-specific locus-resistant virus) in adult stage |
| CN112106733A (en) * | 2020-09-18 | 2020-12-22 | 中国水稻研究所 | Indoor biological assay method for rice planthopper |
| CN112243812A (en) * | 2020-08-27 | 2021-01-22 | 河南科技大学 | Wheat growth period field aphid-resistant screening and identifying device |
| CN114271158A (en) * | 2021-12-30 | 2022-04-05 | 华智生物技术有限公司 | Method for identifying resistance of brown planthopper of rice in field adult stage |
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| CN111557215A (en) * | 2020-02-27 | 2020-08-21 | 四川省农业科学院植物保护研究所 | Identification method and application of rice variety resisting plant hopper and SRBSDV (sequence-specific locus-resistant virus) in adult stage |
| CN111264320A (en) * | 2020-03-06 | 2020-06-12 | 湖南杂交水稻研究中心 | Method for accurately identifying resistance of brown planthopper in adult stage of rice |
| CN112243812A (en) * | 2020-08-27 | 2021-01-22 | 河南科技大学 | Wheat growth period field aphid-resistant screening and identifying device |
| CN112106733A (en) * | 2020-09-18 | 2020-12-22 | 中国水稻研究所 | Indoor biological assay method for rice planthopper |
| CN112106733B (en) * | 2020-09-18 | 2022-03-25 | 中国水稻研究所 | Indoor biological assay method for rice planthopper |
| CN114271158A (en) * | 2021-12-30 | 2022-04-05 | 华智生物技术有限公司 | Method for identifying resistance of brown planthopper of rice in field adult stage |
| CN114271158B (en) * | 2021-12-30 | 2022-12-23 | 华智生物技术有限公司 | Method for identifying resistance of brown planthopper of rice in field adult stage |
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