CN103704102B - The propagation method of the high-order bud of Dendrobium Chrysotoxum Lindl - Google Patents
The propagation method of the high-order bud of Dendrobium Chrysotoxum Lindl Download PDFInfo
- Publication number
- CN103704102B CN103704102B CN201310681398.5A CN201310681398A CN103704102B CN 103704102 B CN103704102 B CN 103704102B CN 201310681398 A CN201310681398 A CN 201310681398A CN 103704102 B CN103704102 B CN 103704102B
- Authority
- CN
- China
- Prior art keywords
- bud
- order
- order bud
- dendrobium chrysotoxum
- chrysotoxum lindl
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- Y02P60/216—
Landscapes
- Cultivation Of Plants (AREA)
Abstract
The invention discloses the propagation method of the high-order bud of a kind of Dendrobium Chrysotoxum Lindl.The method comprises (1), and high-order bud urgees bud: spring mid or late Febuary-March, sprayed one time of nutrition liquid every 2 weeks to Dendrobium Chrysotoxum Lindl complete stool, described nutrient solution adds urea in the liquid of natural pond, to make in nutrient solution Control of Nitrogen Content 0.05 ~ 0.15%; (2) high-order bud cuts off: during root length 2 ~ 4cm, cut off in the lump together with maternal plant bulb 1 ~ 2 joint by high-order bud, otch dipped in 70% mancozeb wettable powder, 600 times of liquid ~ 800 times liquid after 3 ~ 5 seconds, encases root plantation in implant bed crack of stone with liver moss; (3) initial management of high-order bud seedling; (4) final-period management of high-order bud seedling.The inventive method can make high-order bud sprout in a large number, and its germination rate is 80-90%, high-order bud plant percent >=98%, and growth is fast, and within about 2 years, can bloom, reach high yield, its flower production reaches 80kg/ mu ~ 110kg/ mu.
Description
Technical field
The present invention relates to the propagation method of the high-order bud of rare dietotherapeutic medicinal plant Dendrobium Chrysotoxum Lindl.
Background technology
Dendrobium Chrysotoxum Lindl (Dendrobium chrysotoxum Lindl.), have another name called the gold bow stem of noble dendrobium, for the orchid family (Orchidaceace) Dendrobium (Dendrobium) plant, grow nonparasitically upon another plant on the tree of subtropical and tropical zones jungle or on dark and damp rock, being distributed in the ground such as Chinese yunnan province, Guangxi province, is the important forest resources of medicinal plant of China's low latitudes.Dendrobium Chrysotoxum Lindl is used as medicine with its fresh stem or dry stem traditionally, being one of China's conventional medicinal dendrobium kind among the people, promoting the production of body fluid except effect of beneficial stomach, clearing heat and nourishing yin except having, and is also used for the treatment of consumption of body fluid caused by febrile disease traditionally, dry polydipsia, after being ill abnormal heat, the moon hinder the diseases such as order is dark; To throat and gastrointestinal disease, cataract, angiocardiopathy, diabetes, there is obvious curative effect ([1] Bao Xuesheng, along celebrating one's birthday, old vertical drill. Medicinal Dendrobium in China [M]. Shanghai: publishing house of Shanghai Medical Univ, publishing house of Fudan University, 2001.1-49. [2] Yu Meijuan. Dendrobium Sw active ingredient and pharmacological research [J]. Chinese patent drug, 2003,25 (11): 918-921. [3] the Jiangsu colleges of traditional Chinese medicine. Chinese medicine voluminous dictionary [M]. Shanghai: Shanghai science tech publishing house, 1986.586.).
In recent years, along with people are to the understanding of Dendrobium Chrysotoxum Lindl medical value and medicinal effects, and the raising of popular life level, be that medicinal product and the health products price of raw material hits new peak repeatly with Dendrobium Chrysotoxum Lindl, the product amount of asking is also more and more large, and between Product supply amount and the market supply, seriously supply falls short of demand, and its wild resource reduces day by day, seedling demand increases rapidly, and seedling price is also expected to rise every year.
Existing Dendrobium Chrysotoxum Lindl propagation technique, generally adopts division propagation, cottage propagation and group to cultivate seedling.Division propagation has been difficult to meet the need of market due to the minimizing day by day of Dendrobium Chrysotoxum Lindl resource.Cottage propagation and group are cultivated seedling to there is again seedling seedling-slowing stage long, and crop cycle is long, obtains the few defect of effective target Product yields and deficiency.
Summary of the invention
The technical problem to be solved in the present invention be overcome existing Dendrobium Chrysotoxum Lindl resource day by day reduce that its seedling can not meet the demand of industrial scale development, Dendrobium Chrysotoxum Lindl cottage propagation and group cultivate seedling to there is seedling seedling-slowing stage long; crop cycle is long; obtain the few defect of effective target Product yields and deficiency; its objective is and provide one to shorten crop cycle; plant percent, the high-order bud propagation method of the Dendrobium Chrysotoxum Lindl that target product output is high.
For solving the problems of the technologies described above and realizing the object of the invention, the propagation method of the high-order bud of Dendrobium Chrysotoxum Lindl provided by the present invention, comprises the following steps:
(1) high-order bud urgees bud: 1. spring mid or late Febuary-March, sprayed one time of nutrition liquid every 2 weeks to Dendrobium Chrysotoxum Lindl complete stool, described nutrient solution adds urea in the liquid of natural pond, to make in nutrient solution Control of Nitrogen Content at 0.05% ~ 0.15% mass fraction; Or 2. spring mid or late Febuary-March, on Dendrobium Chrysotoxum Lindl pseudobulb top, save the annellation of pocket knife at the dark 1mm ~ 2mm in standardized road, stipes bottom every 2, make the generation of the injured promotion rudiment of pseudobulb, then every 2 weeks, once described nutrient solution is sprayed to Dendrobium Chrysotoxum Lindl complete stool;
(2) high-order bud cuts off: when high-order bud grows to 3 ~ 4 leaves, the long 3 ~ 6cm of stem, aerial root 2 ~ 4, during root length 2 ~ 4cm, high-order bud is cut off in the lump together with maternal plant bulb 1 ~ 2 joint, after cutting, otch dipped in 70% mancozeb wettable powder, 600 times of liquid ~ 800 times liquid after 3 ~ 5 seconds, root plantation is encased in implant bed crack of stone with liver moss, described implant bed is the thickness laid on ground be the particle diameter of 20 ~ 25cm is the rubble of 3-6cm, hangs the sunshade net that shading rate is 40% ~ 50% above implant bed;
(3) initial management of high-order bud seedling: after the plantation of high-order bud in two weeks, air themperature keeps 22 DEG C ~ 28 DEG C, water weekly 1-2 time permeable;
(4) final-period management of high-order bud seedling: after the initial management of high-order bud seedling, execute weekly the nutrient solution described in 1 step (1), weekly water 1 ~ 2 time permeable; To June then and water weekly later 3 ~ 4 times permeable, execute weekly the nutrient solution described in 1 step (1), when transplanting after high-order blastogenesis root.
Described natural pond liquid prepares by following method:
With stalk and cow dung for fermentation raw material, being filled out by fermentation raw material is placed in biogas generating pit, and add water, the mass ratio of stalk and cow dung is 1:1, the water yield is 80% mass fraction of fermentation raw material, pH value controls 7 ~ 8.5, by the C/N ratio in a urea adjustment biogas generating pit between 20 to 1 to 30 to 1, is described natural pond liquid after there is biogas after fermentation excess elimination natural pond slag;
Described in step (4) when can transplant after high-order blastogenesis root can work as high-order blastogenesis root 6-9 bar after transplant.
For more promoting that the bulbil of Dendrobium Chrysotoxum Lindl is sprouted, can be 2. described on Dendrobium Chrysotoxum Lindl pseudobulb top in step (1), the annellation of pocket knife at the dark 1mm ~ 2mm in standardized road, stipes bottom is saved every 2, with the 6-BA solution trowelling of 10mg/L behind cut place, then every 2 weeks, once described nutrient solution is sprayed to Dendrobium Chrysotoxum Lindl complete stool.
The high-order bud of Dendrobium Chrysotoxum Lindl refers to the bud that joint position, Dendrobium Chrysotoxum Lindl pseudobulb middle and upper part is sprouted.
Compared with prior art, the invention has the beneficial effects as follows:
The inventive method can make high-order bud sprout in a large number, and its germination rate is 80%-90%, and what overcome Dendrobium Chrysotoxum Lindl resource reduces the demand that can not meet division propagation day by day.
The high-order bud nursery stock of the inventive method breeding is healthy and strong, and storage rate is high, and survival rate is high; Also effectively shorten the crop cycle from high-order bud transplanting to high yield, output is high, profitable.
High-order bud plant percent >=98% of the inventive method breeding, after transplanting, growth is fast, and within about about 2 years, (23 months-25 months) can bloom, and reach high yield, and the flower production of Dendrobium Chrysotoxum Lindl reaches 80kg/ mu ~ 110kg/ mu.And after traditional cottage propagation and group cultivate transplantation of seedlings, 6 years ~ 8 years ability results Dendrobium Chrysotoxum Lindl fresh flowers need be grown, its Dendrobium Chrysotoxum Lindl fresh flower only has 40kg/ mu ~ 70kg/ mu.The inventive method compares plantlet in vitro, cuttage seeding obtains target output and is advanced by 3-4, compared with prior art, creates unforeseeable technique effect.
Embodiment
Without specified otherwise in following embodiment, it is conventional method.Implement the suitable growth area of place at Dendrobium Chrysotoxum Lindl: essentialspecies is implanted in warm, the moistening and shady and cool environment in tropical and subtropical zone area, vegetative period, year-round average temperature was at 18-23 DEG C, 1 >=280 day frost-free season, more than annual rainfall 1000mm, the area that vegetative period, relative air humidity more than 75% was advisable.
Embodiment 1
The propagation method of the high-order bud of Dendrobium Chrysotoxum Lindl of the present invention, comprises the following steps:
(1) high-order bud urgees bud: February 26 spring-by the end of March, sprayed one time of nutrition liquid every 2 weeks to Dendrobium Chrysotoxum Lindl complete stool, described nutrient solution adds urea in natural pond liquid (namely natural pond liquid be liquid in biogas generating pit), makes nitrogen content in nutrient solution be 0.05% mass fraction; Described natural pond liquid is prepared by following method:
With stalk and cow dung for fermentation raw material, being filled out by fermentation raw material is placed in biogas generating pit, and add water, the mass ratio of stalk and cow dung is 1:1, the water yield added is 80% mass fraction of fermentation raw material, pH value controls at 7-8.0, is 30:1, is described natural pond liquid after there is biogas after fermentation excess elimination natural pond slag by the C/N ratio (C/N) of liquid in a urea adjustment biogas generating pit.
(2) high-order bud cuts off: when high-order bud grows to 3 ~ 4 leaves, the long 3 ~ 6cm of stem, aerial root 2 ~ 4, during root length 2 ~ 4cm, high-order bud is cut off in the lump together with maternal plant bulb 1 ~ 2 joint, after cutting, otch encases root plantation in implant bed crack of stone with liver moss after dipping in 70% mancozeb wettable powder 600 times liquid 3-4 second, and the particle diameter of described implant bed to be the thickness laid on ground be 20 ~ 25cm is the rubble of 3-6cm; The sunshade net that shading rate is 40% is hung above implant bed.
(3) initial management of high-order bud seedling: after the plantation of high-order bud in two weeks, avoid fertilise, air themperature keeps 22 DEG C ~ 25 DEG C, water weekly 1 time permeable.
(4) final-period management of high-order bud seedling: after the initial management of high-order bud seedling, execute weekly the nutrient solution described in 1 step (1), weekly water 1 time permeable; To June then and water weekly later 3 times permeable, execute weekly the nutrient solution described in 1 step (1), when namely transplanting after high-order blastogenesis root 6-9 bar.
Effect: high-order bud is sprouted in a large number, its germination rate reaches more than 80%, and high-order bud plant percent is more than 98%, and after transplanting, growth is fast, and within 23 months, namely bloom, reach high yield, the flower production of Dendrobium Chrysotoxum Lindl on average reaches 80kg/ mu.
Embodiment 2
Embodiment 2 is except following steps difference, and all the other steps are identical with embodiment 1, repeat no more.
(1) high-order bud urgees the bud time in February 16 spring-by the end of March, and in nutrient solution, nitrogen content is 0.15% mass fraction.The C/N ratio (C/N) adjusting liquid in a biogas generating pit with urea is 20:1.
(2) high-order bud cuts off: high-order bud cuts off in the lump together with maternal plant bulb 1 ~ 2 joint, and after cutting, otch dips in 70% mancozeb wettable powder 800 times liquid 4-5 second; The sunshade net that shading rate is 50% is hung above implant bed.
(3) initial management of high-order bud seedling: avoid applying fertilizer in two weeks after the plantation of high-order bud, air themperature keeps 26 DEG C ~ 28 DEG C, water weekly 2 times permeable;
(4) final-period management of high-order bud seedling: after the initial management of high-order bud seedling, execute weekly the nutrient solution described in 1 step (1), weekly water 1 time permeable; To June then and water weekly later 3 times permeable, execute weekly the nutrient solution described in 1 step (1), when namely transplanting after high-order blastogenesis root.
(4) final-period management of high-order bud seedling: after the initial management of high-order bud seedling, water weekly 2 times permeable; To June then and water weekly later 4 times permeable.
Effect: high-order bud is sprouted in a large number, its germination rate reaches more than 80%, and high-order bud plant percent is more than 98%, and after transplanting, growth is fast, and within 25 months, namely bloom, reach high yield, the flower production of Dendrobium Chrysotoxum Lindl on average reaches 80kg/ mu.
Embodiment 3
Embodiment 3 is except following steps difference, and all the other steps are identical with embodiment 1, repeat no more.
(1) high-order bud urgees bud: 2. February 26 spring-by the end of March, on Dendrobium Chrysotoxum Lindl pseudobulb top, save the annellation of pocket knife at the dark 1mm ~ 2mm in standardized road, stipes bottom every 2, make the generation of the injured promotion rudiment of pseudobulb; Every 2 weeks, the nutrient solution described in an embodiment 1 is sprayed to Dendrobium Chrysotoxum Lindl complete stool again after girdling.
Effect: high-order bud is sprouted in a large number, its germination rate reaches 85%, high-order bud plant percent more than 98%, and after transplanting, growth is fast, and within 23 months, namely bloom, reach high yield, the flower production of Dendrobium Chrysotoxum Lindl on average reaches 100kg/ mu.
Embodiment 4
Embodiment 4 is except following steps difference, and all the other steps are identical with embodiment 2, repeat no more.
(1) high-order bud urgees bud: 2. February 16 spring-by the end of March, on Dendrobium Chrysotoxum Lindl pseudobulb top,
Pocket knife is saved after the annellation of the dark 1mm ~ 2mm in standardized road, stipes bottom every 2,6-BA(6-benzyladenine with concentration is 10mg/L) solution trowelling after cut place the generation of rudiment (promote), then sprayed the nutrient solution described in an embodiment 2 every 2 weeks to Dendrobium Chrysotoxum Lindl complete stool.
Effect: high-order bud is sprouted in a large number, its germination rate reaches 98%, and high-order bud plant percent is 100%, and after transplanting, growth is fast, and within 25 months, namely bloom, reach high yield, the flower production of Dendrobium Chrysotoxum Lindl on average reaches 110kg/ mu.
Claims (5)
1. the propagation method of the high-order bud of Dendrobium Chrysotoxum Lindl, comprises the following steps:
(1) high-order bud urgees bud: 1. spring mid or late Febuary-March, sprayed one time of nutrition liquid every 2 weeks to Dendrobium Chrysotoxum Lindl complete stool, described nutrient solution adds urea in the liquid of natural pond, to make in nutrient solution Control of Nitrogen Content at 0.05% ~ 0.15% mass fraction; Or 2. spring mid or late Febuary-March, on Dendrobium Chrysotoxum Lindl pseudobulb top, save pocket knife after the annellation of the dark 1mm ~ 2mm in standardized road, stipes bottom every 2, then every 2 weeks, once described nutrient solution sprayed to Dendrobium Chrysotoxum Lindl complete stool;
(2) high-order bud cuts off: when high-order bud grows to 3 ~ 4 leaves, the long 3 ~ 6cm of stem, aerial root 2 ~ 4, root length 2 ~ 4cm, high-order bud is cut off in the lump together with maternal plant bulb 1 ~ 2 joint, after cutting, otch dipped in 70% mancozeb wettable powder, 600 times of liquid ~ 800 times liquid after 3 ~ 5 seconds, root plantation is encased in implant bed crack of stone with liver moss, described implant bed is to be 20 ~ 25cm at ground laying depth particle diameter is the rubble of 3-6cm, hangs the sunshade net that shading rate is 40% ~ 50% above implant bed;
(3) initial management of high-order bud seedling: after the plantation of high-order bud in two weeks, air themperature keeps 22 DEG C ~ 28 DEG C, water weekly 1-2 time permeable;
(4) final-period management of high-order bud seedling: after the initial management of high-order bud seedling, execute weekly the nutrient solution described in 1 step (1), weekly water 1 ~ 2 time permeable; To June then and water weekly later 3 ~ 4 times permeable, execute weekly the nutrient solution described in 1 step (1), when transplanting after high-order blastogenesis root.
2. the propagation method of the high-order bud of Dendrobium Chrysotoxum Lindl according to claim 1, the natural pond liquid described in step (1) is prepared by following method:
With stalk and cow dung for fermentation raw material, being filled out by fermentation raw material is placed in biogas generating pit, and add water, the mass ratio of stalk and cow dung is 1:1, the water yield is 80% mass fraction of fermentation raw material, pH value controls 7 ~ 8.5, by the C/N ratio in a urea adjustment biogas generating pit between 20 to 1 to 30 to 1, is described natural pond liquid after there is biogas after fermentation excess elimination natural pond slag.
3. the propagation method of the high-order bud of Dendrobium Chrysotoxum Lindl according to claim 1 and 2, described in step (4) is when transplanting after high-order blastogenesis root 6-9 bar when transplanting after high-order blastogenesis root.
4. the propagation method of the high-order bud of Dendrobium Chrysotoxum Lindl according to claim 1 and 2, in the annellation at the dark 1mm ~ 2mm in standardized road, stipes bottom that step (1) is 2. described, with the 6-BA solution trowelling of 10mg/L behind cut place, then every 2 weeks, once described nutrient solution is sprayed to Dendrobium Chrysotoxum Lindl complete stool.
5. the propagation method of the high-order bud of Dendrobium Chrysotoxum Lindl according to claim 3, in the annellation at the dark 1mm ~ 2mm in standardized road, stipes bottom that step (1) is 2. described, with the 6-BA solution trowelling of 10mg/L behind cut place, then every 2 weeks, once described nutrient solution is sprayed to Dendrobium Chrysotoxum Lindl complete stool.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310681398.5A CN103704102B (en) | 2013-12-14 | 2013-12-14 | The propagation method of the high-order bud of Dendrobium Chrysotoxum Lindl |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310681398.5A CN103704102B (en) | 2013-12-14 | 2013-12-14 | The propagation method of the high-order bud of Dendrobium Chrysotoxum Lindl |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103704102A CN103704102A (en) | 2014-04-09 |
| CN103704102B true CN103704102B (en) | 2015-09-16 |
Family
ID=50397780
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310681398.5A Expired - Fee Related CN103704102B (en) | 2013-12-14 | 2013-12-14 | The propagation method of the high-order bud of Dendrobium Chrysotoxum Lindl |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103704102B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106852251A (en) * | 2016-12-27 | 2017-06-16 | 普洱市忠勒佰合农业开发有限公司 | The propagation method of Dendrobium Chrysotoxum Lindl plant division |
| CN107494218B (en) * | 2017-07-18 | 2020-04-28 | 霍山县茧丝绸有限公司 | High-bud seedling culture method for dendrobium |
| CN108157119A (en) * | 2017-12-27 | 2018-06-15 | 康美药业（文山）药材种植管理有限公司 | A kind of Dendrobium Chrysotoxum Lindl culture substrate and preparation method |
| CN114916328B (en) * | 2022-05-31 | 2023-12-01 | 河北鲜达农业科技有限公司 | Dendrobium officinale rooting method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101156535A (en) * | 2006-09-27 | 2008-04-09 | 中国林业科学研究院林业研究所 | Method for controlling flowering and propagation of dendrobium nobile |
| CN101156551A (en) * | 2006-10-11 | 2008-04-09 | 中国林业科学研究院林业研究所 | Dendrobium nobile stem segment cuttage method |
| CN102138420A (en) * | 2011-02-22 | 2011-08-03 | 贵州省农作物品种资源研究所 | Method for cultivating dendrobium fimbriatum |
| CN103283448A (en) * | 2013-05-21 | 2013-09-11 | 江苏圣宏农业科技有限公司 | Method for consistent hole tray seedling cutting for photinia fraseri |
-
2013
- 2013-12-14 CN CN201310681398.5A patent/CN103704102B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101156535A (en) * | 2006-09-27 | 2008-04-09 | 中国林业科学研究院林业研究所 | Method for controlling flowering and propagation of dendrobium nobile |
| CN101156551A (en) * | 2006-10-11 | 2008-04-09 | 中国林业科学研究院林业研究所 | Dendrobium nobile stem segment cuttage method |
| CN102138420A (en) * | 2011-02-22 | 2011-08-03 | 贵州省农作物品种资源研究所 | Method for cultivating dendrobium fimbriatum |
| CN103283448A (en) * | 2013-05-21 | 2013-09-11 | 江苏圣宏农业科技有限公司 | Method for consistent hole tray seedling cutting for photinia fraseri |
Non-Patent Citations (6)
| Title |
|---|
| 春石斛种苗的繁育方法;陈文贞等;《广东农业科学》;20100831;第37卷(第8期);第81-82页 * |
| 春石斛规模化繁殖技术;刘艳芬等;《北方园艺》;20061130(第6期);第145-146页 * |
| 春石斛规模化繁殖要领;李振坚;《中国花卉园艺》;20061130(第22期);第20-22页 * |
| 江泽慧等编著.春石斛繁殖与栽培技术.《石斛兰——资源·生产·应用》.中国林业出版社,2007,第83-92页. * |
| 牛粪、秸秆混合厌氧发酵制沼气研究;姜庆宏等;《可再生能源》;20130131;第31卷(第1期);57-59页 * |
| 药用石斛的人工繁殖与栽培技术;宁铃等;《中国热带农业》;20081231(第6期);第55-57页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103704102A (en) | 2014-04-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103314738B (en) | Dendrobium huoshanense greenhouse planting method | |
| CN100399878C (en) | Water floatation seedling method of cotton | |
| JP2011142903A (en) | Method for raising seedlings of peony | |
| CN103609441B (en) | A kind of blueberry seedling-cultivating method | |
| CN104541970B (en) | The method of northern area outdoor planting rhizoma Gastrodiae | |
| CN103651122B (en) | A kind of bletilla protocorm induction medium | |
| CN100571503C (en) | A kind of elaeagnus mollis asexual reproduction method | |
| CN101946602A (en) | Method for culturing and transplanting seedling of cassava | |
| CN106358773B (en) | The tree-like breeding method of walnut " trunk type " | |
| CN103704102B (en) | The propagation method of the high-order bud of Dendrobium Chrysotoxum Lindl | |
| CN103718935A (en) | Greenhouse cultivation method for butterfly orchid | |
| CN102246697A (en) | Method for growing cell embryos of Dendrobium huoshanense | |
| CN105248280B (en) | Oasis No.1 once-seedling forming tissue culture and rapid propagation method | |
| CN102487677A (en) | Production method for multiple cropping of corn, peanut or soybean in one year | |
| CN105393900A (en) | Built-on-stilt seedbed standardized organic planting method under dendrobium huoshanense forest | |
| CN103535166A (en) | Lonicera japonica nursery stock rapid propagation method | |
| CN103718969A (en) | Regenerated plant in-vitro culture method for Sirindhorn michelia figo | |
| CN106171996B (en) | A kind of rapid propagation method of wild white birch | |
| CN108157155A (en) | A kind of implantation methods of selenium-rich gumbo | |
| CN112119913A (en) | Virus-free propagation method for sweet potatoes | |
| CN103975832B (en) | A kind of white wing silvery birch plantlet in vitro outside sprout-cultivating-bottle method | |
| CN105325214A (en) | Using method of planting culture medium capable of promoting rapid growth of tomatoes | |
| CN105230267B (en) | A kind of root of kudzu vine seedling divides root and stem of certain plants fast breeding method | |
| CN106489477B (en) | A kind of Ecology Chinese podophyllum root method for culturing seedlings | |
| CN101473792B (en) | Tissue culture of Ypsilandra thibetica and planting method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150916 Termination date: 20211214 |