CN107182950A - A kind of parasitic wasp parasitic rate determines device and assay method - Google Patents

A kind of parasitic wasp parasitic rate determines device and assay method Download PDF

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Publication number
CN107182950A
CN107182950A CN201710462553.2A CN201710462553A CN107182950A CN 107182950 A CN107182950 A CN 107182950A CN 201710462553 A CN201710462553 A CN 201710462553A CN 107182950 A CN107182950 A CN 107182950A
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bottle
parasitic
host
parasitic wasp
wasp
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CN201710462553.2A
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CN107182950B (en
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邓瑶
王香萍
张建民
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Yangtze University
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Yangtze University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Catching Or Destruction (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Device is determined the present invention relates to a kind of parasitic wasp parasitic rate, parasitic wasp parasitic rate, which determines device, includes bottle, host in bottle, host plant and parasitic wasp, bottle has relative bottleneck and bottom of bottle, and the body between bottleneck and bottom of bottle, bottle mouth position is provided with bottle cap and the cotton core for being fixed on bottle cap and extending into body, cotton core is provided with hydromel, bottom of bottle is dismountable gauze net, host and host plant are placed in small incubation ware, the receiving space bottom of bottle is placed with some small incubation wares, the pairing of parasitic wasp male and female is released in receiving space.The parasitic wasp parasitic rate, which determines device, can give parasitic wasp supplements simultaneously, and be convenient for changing host and host plant, be unfavorable for the escape of parasitic wasp;In addition, cost is low, and it is simple to operate, it is very suitable for interior and does parasitic rate detection, saves time cost and financial cost.

Description

A kind of parasitic wasp parasitic rate determines device and assay method
Technical field
Device and survey are determined the present invention relates to a kind of parasitic rate determination techniques field, more particularly to a kind of parasitic wasp parasitic rate Determine method.
Background technology
At present, the preventing and treating to farming and animal husbandry insect depends on physical control (sex pheromone, light trap), biological control (release natural enemy, farmland set support animal) and higher effective and lower toxic pesticide preventing and treating.
Bread is the staff of life, eats with An Weixian.The epoch of environmental protection, health and pollution-free food are being advocated, people are more desirable to pass through Ensure the safe high yield and high quality of crops using the Natural control action of pest natural enemy.Therefore, biological control of insect pests has been developed Into inexorable trend, the bio-control method to farming and animal husbandry insect is also more aobvious urgent.The emphasis of farming and animal husbandry biological control of insect pests is to protect Shield utilizes natural enemy, especially parasitic wasp.
Larval phase parasitic wasp is the important parasite of China farming and animal husbandry insect, and it produces ovum in pin main body or body Table, ovum grows in pin main body departs from host to linal-instar larvae, is cocoond in vitro in host and sprouts wings or sent out with host Educate to pupa and directly sprout wings from Host Pupae.All it is remarkably decreased until dead by the food ingestion and vigor of parasitic host in larval phase Die, have more obvious Pest control efficacy to contemporary population quantity or population quantity of future generation, so as to reach the life of Field Pests The purpose of thing preventing and treating.Natural enemy insect is discharged in suitable period, host can obtain effective control in the harm caused by field.Mesh Before, honeybee is raised into collect using the plastic jacket of low capacity more than laboratory, with vial simultaneously place appropriate host and parasitism The mode of honeybee is to breed, and replacing host makes parastic wasp parasitic daily, wherein being easily caused among the process of alternate hose It is damaged, and influence parastic wasp life-span and egg production capacity reduce egg laying amount simultaneously, also easily waste honeybee source.
The content of the invention
The purpose of the present invention is that there is provided a kind of Host transfer made after parasitism and the simple side of replacing for above-mentioned present situation Just and the low parasitic wasp parasitic rate of cost determines device and assay method.
The technical solution adopted by the present invention:A kind of parasitic wasp parasitic rate determines device, is placed in incubator, described to post Raw honeybee parasitic rate, which determines device, includes bottle, the host in the bottle, host plant and parasitic wasp, the bottle level Place, it, which has to be formed in relative bottleneck and bottom of bottle and the body between the bottleneck and bottom of bottle, the body, receives Hold space, the bottle mouth position is provided with bottle cap and the cotton core for being fixed on the bottle cap and extending into the body, the cotton core Provided with hydromel, the bottom of bottle hollow out, provided with dismountable gauze net, the host and host plant are placed on small-sized training Support in ware, the receiving space bottom of the bottle is placed with some small incubation wares, the parasitic wasp male and female pairing release In in the receiving space.
The present invention effect be:The parasitic wasp parasitic rate, which determines device, can give parasitic wasp supplements simultaneously, and conveniently Host and host plant are changed, is unfavorable for the escape of parasitic wasp;In addition, device is simply easily obtained, cost is low, simple to operate, Need space small, as a result accurately, be very suitable for interior and do parasitic rate detection, save time cost and financial cost.
Further, the temperature in the incubator is 26 DEG C ± 1 DEG C, and humidity is 75%.
Further, the bottle is transparent plastic bottle.
Further, the cotton core is horizontally disposed with, and diameter 1cm, length 5-6cm.
Further, the concentration of the hydromel is 10%.
Further, the network interface gap of the gauze net is 5mm*5mm.
Further, the host is 2-3 instar larvaes, is lepidopterous larvae.
Further, release 1-4 is to the parasitic wasp in the bottle, and the parasitic wasp is Aphidiidae parasitic wasp.
The technical solution adopted by the present invention:A kind of parasitic wasp parasitic rate assay method, comprises the following steps:S1:There is provided saturating Bright bottle, the bottle horizontal positioned, it has relative bottleneck and bottom of bottle and between the bottleneck and bottom of bottle Receiving space is formed with body, the body, the bottle mouth position is provided with bottle cap, the bottom of bottle hollow out, provided with dismountable yarn Arrange net;S2:Cotton core is set in the bottle mouth position, the cotton core extends into bottle, and is fixed on the bottle cap, the cotton core It is provided with hydromel;S3:Host and host plant are placed in some small incubation wares, then by some small incubation wares The bottom of the receiving space of the bottle is placed on, and ensures the host and host plant on same horizontal plane;S4:Will The pairing of parasitic wasp male and female is released in the receiving space;And S5:It will be equipped with the bottle of the host, host plant and parasitic wasp Body is positioned in incubator, and the temperature in the incubator is 26 DEG C ± 1 DEG C, and humidity is 75%.
The present invention effect be:The parasitic wasp parasitic rate assay method is simple to operate, it is necessary to space is small, as a result accurately, non- It is very suitable for interior and does parasitic rate detection, saves time cost and financial cost.
Brief description of the drawings
Fig. 1 determines the structural representation of device for a kind of parasitic wasp parasitic rate that the present invention is provided.
Embodiment
The principle and feature of the present invention are described below in conjunction with accompanying drawing, the given examples are served only to explain the present invention, and It is non-to be used to limit the scope of the present invention.
Referring to Fig. 1, determining the structural representation of device for a kind of parasitic wasp parasitic rate that the present invention is provided.The parasitic wasp Parasitic rate determines device and is placed in incubator (not shown), and the temperature in the incubator is 26 DEG C ± 1 DEG C, and humidity is 75%.
The parasitic wasp parasitic rate, which determines device, includes a transparent bottle 1, the host 2 in bottle 1, host plant 3 With parasitic wasp 4.
The horizontal positioned of bottle 1, it has relative bottleneck 11 and bottom of bottle 12 and between bottleneck 11 and bottom of bottle 12 Receiving space 14 is formed with body 13, body 13.In the present embodiment, bottle 1 is transparent plastic bottle.
Bottle cap 15 is provided with bottleneck 11 and the cotton core 16 extended on bottle cap 15 into bottle is fixed on.Cotton core 16 is provided with honey Water, can both originate as the supplements of parasitic wasp 2, can also appropriate moisturizing, can also be provided to a certain degree to parasitic wasp 4 Habitat.Specifically, cotton core 16 is horizontally disposed with, and diameter 1cm, length 5-6cm, be 0.1mm with gauze and thickness cotton Steamed twisted roll system is formed, and its one end removes cotton, only stays gauze to be fixed on bottle cap 15, and the other end is cotton core, and level is horizontal in the air.Put Cotton core is soaked into hydromel before putting, originated as parasitic wasp supplements.In the present embodiment, the concentration of hydromel is 10%.
The hollow out of bottom of bottle 12, provided with dismountable gauze net, the gauze Netcom crosses rubber band and is fixed on body 13.Yu Benshi Apply in example, the network interface gap of the gauze net is 5mm*5mm.
Body 13 is hollow cylindrical shape.
Host 2 and host plant 3 are placed in small incubation ware 5, and the bottom of receiving space 14 of bottle 1 is placed with 1-3 Individual small incubation ware 5, and ensure host 2 and host plant 3 on same horizontal plane.Host 2 is 2-3 instar larvaes, generally Lepidopterous larvae such as Spodopterafrugiperda, diamondback moth etc..
The pairing of the male and female of parasitic wasp 4 is released in receiving space 14, and releasable 1-4 is to parasitic wasp 4 in every bottle, and parasitic wasp 4 is Aphidiidae such as side room disk braconid wasp, Parasitoid Cotesia plutellae etc..
It is to be appreciated that the quantity of host 2 can be according to the placement of the parasitic ability and every bottle of internal excited state 4 of parasitic wasp 4 Quantity is adjusted.If only 1 pair parasitic wasp, host 2 can put 10-20 heads.Host plant 3 adjusts area according to its species, greatly About 4cm2-16cm2
The parasitic wasp parasitic rate can be determined to device when changing host 2 slowly to tilt and by one end of bottle cap 15 towards light Source, parasitic wasp 4 can move to top, remove rubber band and gauze net, place the meeting of small incubation ware 5 of host 2 and host plant 3 Slowly it is slipped at bottom of bottle 12, takes out small incubation ware 5, is then reentered into of the right age host 2 and its host plant 3.Daily Change host 2 and host plant 3 morning, ensure 24h per the secondary parasite time, until the queen bee of parasitic wasp 4 is dead.Now take out Host 2 raises to parasitic wasp 4 to pupate in the body of host 2 with host plant 3 cocoons, and counts the sneak case of parasitic wasp 4.
In this way, the parasitic wasp parasitic rate, which determines device, can give the supplements of parasitic wasp 4 simultaneously, host is convenient for changing again 2 and host plant 3, it is unfavorable for the escape of parasitic wasp 4;In addition, device is simply easily obtained, cost is low, simple to operate, it is necessary to empty Between it is small, as a result accurately, be very suitable for interior and do parasitic rate detection, save time cost and financial cost.
It is to be appreciated that bottle 1 can be 600ml laughable plastic bottle, cut at the 5cm of its bottom with scissors, leave residue Part.Expanded than upper middle part the bottom of laughable plastic bottle, it is easy to which rubber band fixation is not allowed easy to fall off.
The invention further relates to a kind of parasitic wasp parasitic rate assay method, comprise the following steps:
S1:One transparent bottle 1 is provided, the horizontal positioned of bottle 1, it has relative bottleneck 11 and bottom of bottle 12, Yi Jishe It is formed with body 13 between bottleneck 11 and bottom of bottle 12, body 13 at receiving space 14, bottleneck 11 and is provided with bottle cap 15, bottom of bottle 12 hollow outs, and provided with dismountable gauze net.In the present embodiment, bottle 1 is transparent plastic bottle, and the network interface of the gauze net is stitched Gap is 5mm*5mm.
S2:Cotton core 16 is set at bottleneck 11, and cotton core 16 extends into bottle, and is fixed on bottle cap 15, on cotton core 16 Provided with hydromel, can both be originated as the supplements of parasitic wasp 2, can also appropriate moisturizing, to a certain degree can also be to being posted Raw honeybee 4 provides habitat.Specifically, cotton core 16 is horizontally disposed with, and diameter 1cm, length 5-6cm, is to be with gauze and thickness 0.1mm cotton, which rolls, to be formed, and its one end removes cotton, only stays gauze to be fixed on bottle cap 15, and the other end is cotton core, and level is horizontal In the air.Cotton core is soaked into hydromel before placing, originated as parasitic wasp supplements.In the present embodiment, hydromel it is dense Spend for 10%.
S3:Host 2 and host plant 3 are placed in some small incubation wares 5, then some small incubation wares 5 are put Put in the bottom of the receiving space 14 of bottle 1, and ensure host 2 and host plant 3 on same horizontal plane.In the present embodiment In, the quantity of small incubation ware 5 is 1-3, and host 2 is 2-3 instar larvaes, generally lepidopterous larvae such as Spodopterafrugiperda, small Diamond-back moth etc..
S4:The pairing of the male and female of parasitic wasp 4 is released in receiving space 14.It is releasable 1-4 pairs in every bottle in the present embodiment Parasitic wasp 4, parasitic wasp 4 is Aphidiidae such as side room disk braconid wasp, Parasitoid Cotesia plutellae etc..
S5:The bottle 1 that will be equipped with host 2, host plant 3 and parasitic wasp 4 is positioned in incubator, and in the incubator Temperature is 26 DEG C ± 1 DEG C, and humidity is 75%.
It is to be appreciated that the quantity of host 2 can be according to the placement of the parasitic ability and every bottle of internal excited state 4 of parasitic wasp 4 Quantity is adjusted.If only 1 pair parasitic wasp, host 2 can put 10-20 heads.Host plant 3 adjusts area according to its species, greatly About 4cm2-16cm2
The parasitic wasp parasitic rate can be determined to device when changing host 2 slowly to tilt and by one end of bottle cap 15 towards light Source, parasitic wasp 4 can move to top, remove gauze net, place the small incubation ware 5 of host 2 and host plant 3 and can slowly slide To at bottom of bottle 12, small incubation ware 5 is taken out, of the right age host 2 and its host plant 3 is then reentered into.Every morning is changed Host 2 and host plant 3, ensure 24h per the secondary parasite time, until the queen bee of parasitic wasp 4 is dead.The host 2 now taken out uses Host plant 3, which is raised to parasitic wasp 4 to pupate in the body of host 2, to cocoon, and counts the sneak case of parasitic wasp 4.
In this way, the parasitic wasp parasitic rate assay method is simple to operate, it is necessary to space is small, as a result accurately, it is very suitable for room Parasitic rate detection is inside done, time cost and financial cost is saved.
The foregoing is only presently preferred embodiments of the present invention, be not intended to limit the invention, it is all the present invention spirit and Within principle, any modification, equivalent substitution and improvements made etc. should be included in the scope of the protection.

Claims (9)

1. a kind of parasitic wasp parasitic rate determines device, it is placed in incubator, it is characterised in that:The parasitic wasp parasitic rate is surveyed Device is determined including bottle, the host in the bottle, host plant and parasitic wasp, the bottle horizontal positioned, it has Receiving space is formed in relative bottleneck and bottom of bottle and the body between the bottleneck and bottom of bottle, the body, it is described Bottle mouth position is provided with honey provided with bottle cap and the cotton core for being fixed on the bottle cap and extending into the body, the cotton core Water, the bottom of bottle hollow out, provided with dismountable gauze net, the host and host plant are placed in small incubation ware, institute The receiving space bottom for stating bottle is placed with some small incubation wares, and the parasitic wasp male and female pairing is released to the collecting In space.
2. a kind of parasitic wasp parasitic rate as claimed in claim 1 determines device, it is characterised in that:Temperature in the incubator For 26 DEG C ± 1 DEG C, humidity is 75%.
3. a kind of parasitic wasp parasitic rate as claimed in claim 1 determines device, it is characterised in that:The bottle is transparent modeling Expect bottle.
4. a kind of parasitic wasp parasitic rate as claimed in claim 1 determines device, it is characterised in that:The cotton core is horizontally disposed with, And diameter 1cm, length 5-6cm.
5. a kind of parasitic wasp parasitic rate as claimed in claim 1 determines device, it is characterised in that:The concentration of the hydromel is 10%.
6. a kind of parasitic wasp parasitic rate as claimed in claim 1 determines device, it is characterised in that:The network interface seam of the gauze net Gap is 5mm*5mm.
7. a kind of parasitic wasp parasitic rate as claimed in claim 1 determines device, it is characterised in that:The host is 2-3 ages children Worm, is lepidopterous larvae.
8. a kind of parasitic wasp parasitic rate as claimed in claim 1 determines device, it is characterised in that:Release 1-4 in the bottle To the parasitic wasp, the parasitic wasp is Aphidiidae parasitic wasp.
9. a kind of parasitic wasp parasitic rate assay method, it is characterised in that comprise the following steps:
S1:Transparent bottle is provided, the bottle horizontal positioned, it has relative bottleneck and bottom of bottle and located at the bottle Body mouthful between bottom of bottle, is formed with receiving space in the body, and the bottle mouth position is provided with bottle cap, the bottom of bottle hollow out, Provided with dismountable gauze net;
S2:Cotton core is set in the bottle mouth position, the cotton core extends into bottle, and is fixed on the bottle cap, the cotton core It is provided with hydromel;
S3:Host and host plant are placed in some small incubation wares, then some small incubation wares are placed on institute The bottom of the receiving space of bottle is stated, and ensures the host and host plant on same horizontal plane;
S4:The pairing of parasitic wasp male and female is released in the receiving space;And
S5:The bottle that will be equipped with the host, host plant and parasitic wasp is positioned in incubator, and the temperature in the incubator Spend for 26 DEG C ± 1 DEG C, humidity is 75%.
CN201710462553.2A 2017-06-19 2017-06-19 Parasitic wasp parasitic rate measuring device and method Active CN107182950B (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108522435A (en) * 2018-06-08 2018-09-14 河南农业大学 A kind of device and parasitic ability evaluation method of detection the parasitism performance of parasitic wasp
CN111838077A (en) * 2019-04-28 2020-10-30 安庆师范大学 T-shaped observation room with parasitic wasp parasitic rate enhanced by volatile allelochemicals and observation method thereof
CN111838076A (en) * 2019-04-28 2020-10-30 安庆师范大学 Cylindrical observation chamber for enhancing parasitic rate of parasitic wasps by non-volatile allelochemicals and observation method thereof

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CN204090802U (en) * 2014-09-01 2015-01-14 浙江大学 A kind of extensive raising diamond-back moth adult device
CN105248382A (en) * 2015-11-01 2016-01-20 贵州大学 Breeding method and device of orius similis
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108522435A (en) * 2018-06-08 2018-09-14 河南农业大学 A kind of device and parasitic ability evaluation method of detection the parasitism performance of parasitic wasp
CN111838077A (en) * 2019-04-28 2020-10-30 安庆师范大学 T-shaped observation room with parasitic wasp parasitic rate enhanced by volatile allelochemicals and observation method thereof
CN111838076A (en) * 2019-04-28 2020-10-30 安庆师范大学 Cylindrical observation chamber for enhancing parasitic rate of parasitic wasps by non-volatile allelochemicals and observation method thereof

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