CN107182950B - Parasitic wasp parasitic rate measuring device and method - Google Patents
Parasitic wasp parasitic rate measuring device and method Download PDFInfo
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- CN107182950B CN107182950B CN201710462553.2A CN201710462553A CN107182950B CN 107182950 B CN107182950 B CN 107182950B CN 201710462553 A CN201710462553 A CN 201710462553A CN 107182950 B CN107182950 B CN 107182950B
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- 230000003071 parasitic effect Effects 0.000 title claims abstract description 82
- 238000000034 method Methods 0.000 title claims abstract description 9
- 241001481304 Vespoidea Species 0.000 claims abstract description 43
- 229920000742 Cotton Polymers 0.000 claims abstract description 34
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 235000012907 honey Nutrition 0.000 claims abstract description 11
- 241000257303 Hymenoptera Species 0.000 claims description 11
- 230000024241 parasitism Effects 0.000 claims description 10
- 229920003023 plastic Polymers 0.000 claims description 8
- 241000255777 Lepidoptera Species 0.000 claims description 3
- 230000003203 everyday effect Effects 0.000 claims description 2
- 241000256856 Vespidae Species 0.000 claims 1
- 229940071160 cocoate Drugs 0.000 claims 1
- 235000013305 food Nutrition 0.000 abstract description 10
- 238000001514 detection method Methods 0.000 abstract description 5
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 239000013589 supplement Substances 0.000 abstract description 3
- 241000607479 Yersinia pestis Species 0.000 description 7
- 241000256816 Braconidae Species 0.000 description 6
- 241001465754 Metazoa Species 0.000 description 5
- 241000500437 Plutella xylostella Species 0.000 description 4
- 235000013601 eggs Nutrition 0.000 description 4
- 239000004033 plastic Substances 0.000 description 4
- 241000256251 Spodoptera frugiperda Species 0.000 description 2
- 239000000571 coke Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000001418 larval effect Effects 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000017448 oviposition Effects 0.000 description 2
- 238000005096 rolling process Methods 0.000 description 2
- 238000002791 soaking Methods 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 1
- 239000000877 Sex Attractant Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 235000012631 food intake Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 229940056582 human hair preparation Drugs 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Catching Or Destruction (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to a parasitic wasp parasitic rate measuring device which comprises a bottle body, a host plant and a parasitic wasp, wherein the host, the host plant and the parasitic wasp are arranged in the bottle body, the bottle body is provided with a bottle opening, a bottle bottom and a bottle body arranged between the bottle opening and the bottle bottom, the bottle opening is provided with a bottle cover and a cotton core which is fixed on the bottle cover and extends into the bottle body, honey water is arranged on the cotton core, the bottle bottom is a detachable gauze net, the host and the host plant are placed in small-sized culture dishes, the bottom of a containing space of the bottle body is provided with a plurality of small-sized culture dishes, and the parasitic wasp is released in the containing space in a male-female matching manner. The parasitic wasp parasitic rate measuring device can supplement food for parasitic wasps at the same time, and is convenient for replacing hosts and host plants, thereby being not beneficial to the escape of the parasitic wasps; in addition, the method is low in cost, simple to operate, very suitable for indoor detection of the parasitic rate, and capable of saving time cost and economic cost.
Description
Technical Field
The invention relates to the technical field of parasitic rate measurement, in particular to a parasitic rate measurement device and a parasitic rate measurement method for parasitic bees.
Background
At present, the control of pests in agriculture and animal husbandry mainly depends on physical control (sex pheromone and light trapping), biological control (natural enemies are released, animals are raised in farmlands) and high-efficiency and low-toxicity pesticide control.
People eat food as day and eat food as first. In the era of advocating environmental protection, health and green food, people hope to ensure the safety, high yield and high quality of crops by utilizing the natural control action of natural enemies of pests. Therefore, the development of biological pest control has become an inevitable trend, and biological pest control measures for agriculture and animal husbandry are more urgent. The important point of biological control of pests in agriculture and animal husbandry is to protect and utilize natural enemies, especially parasitic wasps.
Parasitic wasps in larval stage are important parasitic natural enemies of pests in agriculture and animal husbandry in China, eggs are laid in or on the body surface of a host, and after the eggs grow and develop to end-aged larvae in the host, the eggs are separated from the host, and the eggs cocoon and emerge outside the host or develop to pupae along with the host and emerge from the pupae of the host directly. The food intake and the vitality of the hosts parasitized in the larval stage are obviously reduced until the hosts die, and the number of the current generation population or the next generation population is obviously controlled, so that the aim of biological control of field pests is fulfilled. The natural enemy insects are released in a proper period, and the harm caused by the hosts in the field can be effectively controlled. At present, a small-capacity plastic cover is mostly adopted in a laboratory to collect raised bees, a proper amount of hosts and parasitic bees are placed in a glass bottle to breed, and a host is replaced every day to parasitize parasitic peaks, wherein damage is easily caused in the process of transferring the hosts, the service life of the parasitic peaks and the egg laying capacity are influenced, the egg laying amount is reduced, and bee sources are easily wasted.
Disclosure of Invention
The present invention has been made in view of the above circumstances, and an object of the present invention is to provide a parasitic wasp parasitic ratio measuring apparatus and a parasitic wasp parasitic ratio measuring method which are capable of easily and conveniently transferring and replacing a parasitic wasp host and are low in cost.
The technical scheme adopted by the invention is as follows: a parasitic wasp parasitic rate measuring device is placed in an incubator and comprises a bottle body, hosts, host plants and parasitic wasps, wherein the hosts, the host plants and the parasitic wasps are arranged in the bottle body, the bottle body is horizontally placed and provided with a bottle opening, a bottle bottom and a bottle body, the bottle body is arranged between the bottle opening and the bottle bottom, a containing space is formed in the bottle body, a bottle cover and a cotton core which is fixed on the bottle cover and extends into the bottle body, honey water is arranged on the cotton core, the bottle bottom is hollow and is provided with a detachable gauze net, the hosts and the host plants are placed in small culture dishes, the small culture dishes are placed at the bottom of the containing space of the bottle body, and the parasitic wasps are released in the containing space in a male-female matching mode.
The invention has the following effects: the parasitic wasp parasitic rate measuring device can supplement food for parasitic wasps at the same time, and is convenient for replacing hosts and host plants, thereby being not beneficial to the escape of the parasitic wasps; in addition, the device is simple and easy to obtain, low in cost, simple to operate, small in required space and accurate in result, is very suitable for indoor detection of the parasitic rate, and saves time cost and economic cost.
Further, the temperature in the incubator is 26 ℃ +/-1 ℃, and the humidity is 75%.
Further, the bottle body is a transparent plastic bottle.
Furthermore, the cotton core is horizontally arranged, and has the diameter of 1cm and the length of 5-6 cm.
Further, the concentration of the honey water is 10%.
Furthermore, the gap of the gauze is 5mm × 5 mm.
Further, the host is larva of 2-3 instar, such as Lepidoptera larva.
Further, 1-4 pairs of parasitic wasps are released in the bottle body, and the parasitic wasps are parasitic wasps in the family of braconidae.
The technical scheme adopted by the invention is as follows: s1, providing a transparent bottle body which is horizontally arranged and is provided with a bottle mouth, a bottle bottom and a bottle body arranged between the bottle mouth and the bottle bottom, wherein an accommodating space is formed in the bottle body, a bottle cap is arranged at the bottle mouth, the bottle bottom is hollow, and a detachable gauze net is arranged at the bottle bottom; s2: a cotton core is arranged at the bottle mouth, extends inwards the bottle and is fixed on the bottle cover, and honey water is arranged on the cotton core; s3: placing the hosts and the host plants in a plurality of small culture dishes, placing the small culture dishes at the bottom of the accommodating space of the bottle body, and ensuring that the hosts and the host plants are on the same horizontal plane; s4: releasing the male and female pairs of parasitic wasps into the accommodating space; and S5: and placing the bottle body filled with the host, the host plant and the parasitic wasp into an incubator, wherein the temperature in the incubator is 26 +/-1 ℃, and the humidity is 75%.
The invention has the following effects: the parasitic wasp parasitic rate measuring method is simple to operate, small in required space, accurate in result, quite suitable for indoor parasitic rate detection, and capable of saving time cost and economic cost.
Drawings
Fig. 1 is a schematic structural diagram of a parasitic ratio measuring apparatus for parasitic bees according to the present invention.
Detailed Description
The principles and features of this invention are described below in conjunction with the following drawings, which are set forth by way of illustration only and are not intended to limit the scope of the invention.
Fig. 1 is a schematic structural diagram of a parasitic ratio measuring device for parasitic bees according to the present invention. The parasitic wasp parasitism rate measuring device is placed in an incubator (not shown), and the temperature in the incubator is 26 ℃. + -. 1 ℃ and the humidity is 75%.
The parasitic wasp parasitic rate measuring device comprises a transparent bottle body 1, a host 2 arranged in the bottle body 1, a host plant 3 and a parasitic wasp 4.
The bottle body 1 is horizontally arranged and is provided with a bottle opening 11, a bottle bottom 12 and a bottle body 13 arranged between the bottle opening 11 and the bottle bottom 12, and a containing space 14 is formed in the bottle body 13. In the present embodiment, the bottle body 1 is a transparent plastic bottle.
The bottle mouth 11 is provided with a bottle cap 15 and a cotton core 16 which is fixed on the bottle cap 15 and extends into the bottle. The honey water is arranged on the cotton core 16, which can be used as a supplementary food source for the parasitic wasps 2, can also be moderately moisturized, and can provide a habitat for the parasitic wasps 4 to a certain extent. Specifically, the cotton core 16 is horizontally arranged, has a diameter of 1cm and a length of 5-6cm, and is formed by rolling cotton with a thickness of 0.1mm and gauze, wherein cotton is removed from one end of the cotton core, only the gauze is fixed on the bottle cap 15, and the cotton core is arranged at the other end of the cotton core and horizontally and transversely arranged in the air. Before placing, soaking cotton core in Mel water as food source for parasitic wasp. In this example, the concentration of the honey water is 10%.
The bottle bottom 12 is hollowed out and provided with a detachable gauze net which is fixed on the bottle body 13 through a rubber band. In this embodiment, the gap of the gauze mesh is 5mm by 5 mm.
The bottle body 13 is a hollow cylinder.
The hosts 2 and the host plants 3 are placed in the small-sized culture dish 5, 1-3 small-sized culture dishes 5 are placed at the bottom of the accommodating space 14 of the bottle body 1, and the hosts 2 and the host plants 3 are ensured to be on the same horizontal plane. Host 2 is larva of 2-3 years old, typically Lepidoptera larva such as Spodoptera frugiperda, Plutella xylostella, etc.
The parasitic wasps 4 are released in the accommodating space 14 in a male-female matching manner, 1-4 pairs of parasitic wasps 4 can be released in each bottle, and the parasitic wasps 4 are braconidae such as border room crinis carbonisatus, plutella xylostella braconidae and the like.
It will be appreciated that the number of hosts 2 may be adjusted according to the parasitic capacity of parasitic wasps 4 and the number of parasitic wasps 4 placed in each bottle. If there are only 1 pair of parasitic wasps, host 2 can put 10-20 heads. The host plant 3 has an area of about 4cm adjusted according to its species 2 -16cm 2 。
When the host 2 is replaced, the parasitic wasp parasitism rate measuring device can be slowly inclined, one end of the bottle cover 15 faces to the light source, the parasitic wasps 4 can move upwards, the rubber band and the gauze net are taken down, the small culture dish 5 for placing the host 2 and the host plant 3 slowly slides to the bottle bottom 12, the small culture dish 5 is taken out, and then the host 2 and the host plant 3 with the proper age are placed again. The host 2 and the host plant 3 are replaced every morning, and the parasitic time is guaranteed for 24 hours each time until the female bees of the parasitic bees 4 die. At this time, the taken host 2 is raised by the host plant 3 until the parasitic wasp 4 pupates and cocoons in the host 2, and the parasitic condition of the parasitic wasp 4 is counted.
Therefore, the parasitic rate measuring device for parasitic wasps can not only supplement food for parasitic wasps 4, but also facilitate the replacement of hosts 2 and host plants 3, and is not beneficial to the escape of parasitic wasps 4; in addition, the device is simple and easy to obtain, low in cost, simple to operate, small in required space and accurate in result, is very suitable for indoor detection of the parasitic rate, and saves time cost and economic cost.
It will be appreciated that the body 1 may be a 600ml cola plastic bottle, the bottom 5cm of which is cut off with scissors, leaving the remainder. The bottom of the coke plastic bottle is expanded compared with the upper middle part, so that the coke plastic bottle is easy to fix by the rubber band and is not easy to fall off.
The invention also relates to a parasitic rate measuring method of parasitic wasps, which comprises the following steps:
s1, providing a transparent bottle body 1, wherein the bottle body 1 is horizontally placed and is provided with a bottle mouth 11, a bottle bottom 12 and a bottle body 13 arranged between the bottle mouth 11 and the bottle bottom 12, a containing space 14 is formed in the bottle body 13, a bottle cap 15 is arranged at the bottle mouth 11, the bottle bottom 12 is hollow, and a detachable gauze net is arranged. In this embodiment, the bottle body 1 is a transparent plastic bottle, and the gap between the gauze and the gauze is 5mm by 5 mm.
S2: the cotton core 16 is arranged at the bottle opening 11, the cotton core 16 extends into the bottle and is fixed on the bottle cap 15, and honey water is arranged on the cotton core 16, so that the cotton core can be used as a supplementary food source for the parasitic wasps 2, can be moderately moisturized, and can provide a habitat for the parasitic wasps 4 to a certain degree. Specifically, the cotton core 16 is horizontally arranged, has a diameter of 1cm and a length of 5-6cm, and is formed by rolling cotton with a thickness of 0.1mm and gauze, wherein cotton is removed from one end of the cotton core, only the gauze is fixed on the bottle cap 15, and the cotton core is arranged at the other end of the cotton core and horizontally and transversely arranged in the air. Before placing, soaking cotton core in Mel water as food source for parasitic wasp. In this example, the concentration of the honey water was 10%.
S3: the hosts 2 and the host plants 3 are placed in a plurality of small petri dishes 5, and the small petri dishes 5 are placed at the bottom of the accommodating space 14 of the bottle body 1, so that the hosts 2 and the host plants 3 are kept on the same horizontal plane. In this example, the number of the small petri dishes 5 is 1-3, and the hosts 2 are 2-3 instar larvae, which are generally lepidopteran larvae such as spodoptera frugiperda, plutella xylostella, and the like.
S4: the parasitic wasp 4 male and female pairs are released into the housing space 14. In this embodiment, 1-4 pairs of parasitic wasps 4 can be released from each bottle, and the parasitic wasps 4 are braconidae such as fringed braconidae and plutella xylostella braconidae.
S5: the bottle 1 containing the host 2, the host plant 3 and the parasitic wasp 4 is placed in an incubator with a temperature of 26 ℃ + -1 ℃ and a humidity of 75%.
It will be appreciated that the number of hosts 2 may be adjusted according to the parasitic capacity of parasitic wasps 4 and the number of parasitic wasps 4 placed in each bottle. If there are only 1 pair of parasitic wasps, host 2 can put 10-20 heads. The area of the host plant 3 is adjusted to about 4cm according to its species 2 -16cm 2 。
When the host 2 is replaced, the parasitic wasp parasitism rate measuring device can be slowly inclined, one end of the bottle cover 15 faces to the light source, the parasitic wasps 4 can move upwards, the gauze net is taken down, the small culture dish 5 for placing the host 2 and the host plant 3 slowly slides to the bottle bottom 12, the small culture dish 5 is taken out, and then the host 2 and the host plant 3 with the proper age are placed again. And (3) replacing the host 2 and the host plant 3 every morning, and ensuring the parasitic time for 24 hours each time until the female bees of the parasitic bees 4 die. At this time, the taken host 2 is raised by the host plant 3 until the parasitic wasp 4 pupates and cocoons in the host 2, and the parasitic condition of the parasitic wasp 4 is counted.
Therefore, the parasitic rate measuring method for the parasitic wasps is simple to operate, small in required space and accurate in result, is very suitable for indoor parasitic rate detection, and saves time cost and economic cost.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and should not be taken as limiting the scope of the present invention, which is intended to cover any modifications, equivalents, improvements, etc. within the spirit and scope of the present invention.
Claims (9)
1. A parasitic bee parasitic rate survey device, places in the incubator, its characterized in that: the parasitic bee parasitic rate measuring device comprises a bottle body, a host plant and a parasitic bee, wherein the host, the host plant and the parasitic bee are arranged in the bottle body, the bottle body is horizontally arranged and provided with a bottle opening, a bottle bottom and a bottle body arranged between the bottle opening and the bottle bottom, a containing space is formed in the bottle body, the bottle opening is provided with a bottle cap and a cotton core which is fixed on the bottle cap and extends into the bottle body, honey water is arranged on the cotton core, the bottle bottom is hollow and is provided with a detachable gauze net, the gauze net is fixed on the bottle body through a rubber band, the host plant and the host plant are placed in small culture dishes, the small culture dishes are placed at the bottom of the containing space of the bottle body, and the parasitic bees are released in the containing space in a male-female matching manner; when the host is replaced, the parasitic wasp parasitizing rate measuring device is slowly inclined, one end of the bottle cover faces to the light source, the parasitic wasps move upwards, the rubber band and the gauze net are taken down, the small culture dish for placing the parasitic wasps and the host plants slowly slides to the bottom of the bottle, the small culture dish is taken out, then the hosts and the host plants with the proper age are placed again, the hosts and the host plants are replaced in the morning every day, the parasitizing time is guaranteed for 24 hours each time until the female wasps of the parasitic wasps die, the taken hosts are bred by the host plants until the parasitic wasps pupate and cocoate in the hosts, and the parasitizing condition of the parasitic wasps is counted.
2. A parasitic wasp parasitism rate determining apparatus, as claimed in claim 1, wherein: the temperature in the incubator is 26 +/-1 ℃, and the humidity is 75%.
3. A parasitic wasp parasitism rate determining apparatus, as claimed in claim 1, wherein: the bottle body is a transparent plastic bottle.
4. A parasitic wasp parasitism rate determining apparatus, as claimed in claim 1, wherein: the cotton core is horizontally arranged, and the diameter of the cotton core is 1cm, and the length of the cotton core is 5-6 cm.
5. A parasitic wasp parasitism rate determining apparatus, as claimed in claim 1, wherein: the concentration of the honey water is 10%.
6. A parasitic wasp parasitism rate determining apparatus, as claimed in claim 1, wherein: the gap of the gauze mesh is 5mm by 5 mm.
7. A parasitic wasp parasitism rate determining apparatus, as claimed in claim 1, wherein: the host is larva of 2-3 years old, and is larva of Lepidoptera.
8. A parasitic wasp parasitism rate determining apparatus, as claimed in claim 1, wherein: 1-4 pairs of parasitic wasps are released in the bottle body, and the parasitic wasps are parasitic wasps in the family of cocoon bee.
9. A parasitic-wave parasitic-ratio measuring method including a parasitic-wave parasitic-ratio measuring apparatus according to any one of claims 1 to 8, characterized by comprising the steps of:
s1, providing a transparent bottle body, wherein the bottle body is horizontally placed and is provided with a bottle mouth, a bottle bottom and a bottle body, the bottle mouth and the bottle bottom are opposite, the bottle body is arranged between the bottle mouth and the bottle bottom, a containing space is formed in the bottle body, a bottle cap is arranged at the bottle mouth, the bottle bottom is hollow, and a detachable gauze net is arranged at the bottle bottom;
s2: a cotton core is arranged at the bottle mouth, extends inwards the bottle and is fixed on the bottle cover, and honey water is arranged on the cotton core;
s3: placing the hosts and the host plants in a plurality of small culture dishes, placing the small culture dishes at the bottom of the accommodating space of the bottle body, and ensuring that the hosts and the host plants are on the same horizontal plane;
s4: releasing the male and female pairs of parasitic bees into the accommodating space; and
s5: and placing the bottle body filled with the host, the host plant and the parasitic wasp into an incubator, wherein the temperature in the incubator is 26 +/-1 ℃, and the humidity is 75%.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710462553.2A CN107182950B (en) | 2017-06-19 | 2017-06-19 | Parasitic wasp parasitic rate measuring device and method |
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| CN201710462553.2A CN107182950B (en) | 2017-06-19 | 2017-06-19 | Parasitic wasp parasitic rate measuring device and method |
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| CN107182950A CN107182950A (en) | 2017-09-22 |
| CN107182950B true CN107182950B (en) | 2022-09-13 |
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| CN108522435A (en) * | 2018-06-08 | 2018-09-14 | 河南农业大学 | A kind of device and parasitic ability evaluation method of detection the parasitism performance of parasitic wasp |
| CN111838077A (en) * | 2019-04-28 | 2020-10-30 | 安庆师范大学 | T-shaped observation room with parasitic wasp parasitic rate enhanced by volatile allelochemicals and observation method thereof |
| CN111838076A (en) * | 2019-04-28 | 2020-10-30 | 安庆师范大学 | Cylindrical observation chamber for enhancing parasitic rate of parasitic wasps by non-volatile allelochemicals and observation method thereof |
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| CN103918613B (en) * | 2014-04-23 | 2016-04-27 | 安徽师范大学 | A method for parasitic wasp artificial propagation in pupa time, and raise bees box and preparation method |
| CN204090802U (en) * | 2014-09-01 | 2015-01-14 | 浙江大学 | A kind of extensive raising diamond-back moth adult device |
| CN105248382A (en) * | 2015-11-01 | 2016-01-20 | 贵州大学 | Breeding method and device of orius similis |
| CN205682214U (en) * | 2016-06-03 | 2016-11-16 | 贵州大学 | A kind of breeding apparatus of cacac moth |
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