CN111838076A - Cylindrical observation chamber for enhancing parasitic rate of parasitic wasps by non-volatile allelochemicals and observation method thereof - Google Patents
Cylindrical observation chamber for enhancing parasitic rate of parasitic wasps by non-volatile allelochemicals and observation method thereof Download PDFInfo
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- CN111838076A CN111838076A CN201910382030.6A CN201910382030A CN111838076A CN 111838076 A CN111838076 A CN 111838076A CN 201910382030 A CN201910382030 A CN 201910382030A CN 111838076 A CN111838076 A CN 111838076A
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- 239000003627 allelochemical Substances 0.000 title claims abstract description 30
- 230000003071 parasitic effect Effects 0.000 title claims abstract description 28
- 238000000034 method Methods 0.000 title claims abstract description 20
- 230000002708 enhancing effect Effects 0.000 title claims abstract description 18
- 241001481304 Vespoidea Species 0.000 title claims description 11
- 238000002474 experimental method Methods 0.000 claims abstract description 7
- 241001122767 Theaceae Species 0.000 claims description 68
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 claims description 55
- 235000013601 eggs Nutrition 0.000 claims description 41
- 241000257303 Hymenoptera Species 0.000 claims description 31
- 241001414720 Cicadellidae Species 0.000 claims description 30
- 239000011248 coating agent Substances 0.000 claims description 15
- 238000000576 coating method Methods 0.000 claims description 15
- 230000017448 oviposition Effects 0.000 claims description 15
- 150000001875 compounds Chemical class 0.000 claims description 11
- 239000002904 solvent Substances 0.000 claims description 11
- 238000012258 culturing Methods 0.000 claims description 10
- 241000238631 Hexapoda Species 0.000 claims description 6
- 201000010099 disease Diseases 0.000 claims description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 5
- 239000011521 glass Substances 0.000 claims description 5
- 239000003960 organic solvent Substances 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 5
- 230000001105 regulatory effect Effects 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 241000245665 Taraxacum Species 0.000 claims description 4
- 239000003337 fertilizer Substances 0.000 claims description 2
- 206010061217 Infestation Diseases 0.000 claims 1
- 230000003014 reinforcing effect Effects 0.000 claims 1
- 230000036531 allelopathy Effects 0.000 description 3
- 230000006399 behavior Effects 0.000 description 3
- 208000015181 infectious disease Diseases 0.000 description 3
- 230000002458 infectious effect Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 230000000750 progressive effect Effects 0.000 description 2
- 241000931705 Cicada Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 239000003620 semiochemical Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a cylindrical observation chamber for enhancing parasitic rate of non-volatile allelochemicals and an observation method thereof. The method is convenient to operate, simple in experiment and capable of well researching and observing the influence of the non-volatile allelochemicals on the aspect of enhancing the control of the parasitic natural enemies, so that the theoretical scientific research and practical application and popularization of the allelochemicals are accelerated.
Description
Technical Field
The invention relates to the field of biology, in particular to a cylindrical observation chamber for enhancing parasitic rate of parasitic wasps by using a non-volatile allelopathy compound and an observation method thereof.
Background
In the biological control practice, people dreams for a long time to regulate and control the behaviors between pests and natural enemies, along with research and technical innovation, more and more action mechanisms of insect behavior inducement (chemistry, color, sound and the like) are disclosed, and research shows that whether insects search host plants or natural enemies search hosts, the insects have a fixed behavior sequence which is generally divided into stages of orientation, positioning, discovery, acceptance and the like, the process is called 'action reaction chain' and some chemical substance from the interaction of host, host plant or both plays an important role in the action process, these intervarietal semiochemicals, known as allelochemicals, non-volatile allelochemicals play a key role in the specific location of the parasite bee locking cicada eggs, therefore, the pest control effect of the parasitic wasps can be improved by using the non-volatile allelopathy compound by utilizing the natural enemy enhancement technology of the insects.
However, the existing allelochemicals do not have good research and observation methods for enhancing the control of the parasitic natural enemies, and therefore, a cylindrical observation chamber and an observation method for enhancing the parasitic rate of parasitic bees by the non-volatile allelochemicals are provided.
Disclosure of Invention
Based on the technical problems in the background art, the invention provides a cylindrical observation chamber with enhanced parasitic rate of parasitic wasps by using a non-volatile allelopathy compound and an observation method thereof.
The cylindrical observation chamber for enhancing parasitic rate of non-volatile allelochemicals comprises a circular support base, a cylindrical cover and air holes, and is characterized in that the cylindrical cover is arranged on the support base, the air holes are formed in the top of the cylindrical cover, and a release table and four object stages are arranged on the top of the circular support base.
An observation method for enhancing parasitic rate of parasitic wasps by using non-volatile allelochemicals, comprising the following steps:
s1: preparation of the experiment: a plurality of tea seedlings, a plurality of leafhoppers and a plurality of female bees were prepared, and a allelochemicals and n-hexane were purchased from a regular supplier.
S2: spawning: selecting leafhoppers and tea seedlings in the S1, selecting a proper greenhouse, putting the tea seedlings into the greenhouse, putting the leafhoppers on tea tips of the tea seedlings to lay eggs, selecting 3-5 branches of 1 bud and 4 leaf tea tips with eggs after 24 hours of laying eggs, and taking 2 groups of tea tips with 50 egg laying holes in total;
S3: and (3) regulating the culture environment: the cylindrical observation room is placed in a special laboratory, a fluorescent lamp is prepared and positioned right above the cylindrical observation room, light can be uniformly irradiated into the cylindrical observation room, the temperature of the laboratory is controlled to be 24-26 ℃ by a temperature and humidity controller, and the relative humidity is 60% -80%.
S4: and (3) concentration adjustment: taking out the allelochemicals described in S1, and preparing concentration gradient 10 with n-hexane-2g/mL、10-4g/mL、10-6g/mL of a solvent A to be detected, putting the solvent A to be detected into a first storage cup, and putting n-hexane for comparison into a second storage cup;
s5: point coating: selecting a plurality of identical brush brushes, spot-coating the solvent A to be detected in the first storage beaker in the S4 around the egg-laying holes on one group of the tea tips with the eggs to obtain a treatment group B, and spot-coating the contrast n-hexane in the second storage beaker in the S4 around the egg-laying holes on the other group of the tea tips with the eggs to obtain a treatment group C.
S6: releasing bees: and (3) placing the treated group B and the treated group C treated in the step (S5) on symmetrical object stages in a cylindrical observation chamber, taking 4 female bees in the step (S1) by using a finger-type tube, placing the female bees on a central release table in the cylindrical observation chamber, enabling the tube orifice to face upwards, changing the treated group B and the treated group C for 1 hour, repeating the operations for 2 times, changing the position for 2 times, and taking out the treated group B and the treated group C after the female bees are placed for 12 hours.
S7: culturing: placing the treatment group B and the treatment group C taken out of the S6 into a light incubator for culturing for 5 days to respectively obtain a culture D and a culture E;
s8: counting parasitic eggs: the culture D and the culture E in the S7 were placed under a microscope for observation, and the parasitized eggs of the leafhopper were peeled from the young stem of the tea shoot to count the parasitized rate.
Preferably, the cylindrical observation chamber is made of colorless organic glass, and the diameter of the cylindrical observation chamber is 40-80 cm.
Preferably, the fluorescent lamp in S3 is a 40W fluorescent lamp, and the temperature and humidity controller in S3 is TDK 0302.
Preferably, the model of the microscope in S8 is RW 0850.
Preferably, the tea seedlings in the step S1 are same in tea age and all grow for 3 years, the tea seedlings grow uniformly, clonal potted tea seedlings which are not damaged by diseases and insects are subjected to fertilizer and water management according to the same standard, leafhoppers are all obtained by progressive propagation in a greenhouse, female bees are all obtained by progressive propagation in the greenhouse by using eggs of leafhoppers as hosts, and the female bees are all female bees which emerge for 24 hours.
Preferably, the allelochemicals in S1 are nonvolatile and soluble in organic solvent n-hexane.
The invention has the beneficial effects that: the method is convenient to operate, simple in experiment and capable of well researching and observing the influence of the non-volatile allelochemicals on the aspect of enhancing the control of the parasitic natural enemies, so that the theoretical scientific research and practical application and popularization of the allelochemicals are accelerated.
Drawings
FIG. 1 is a schematic view of a cylindrical observation chamber according to the present invention.
In the figure: 1. a circular support base; 2. a cylindrical cage; 3. and (4) air holes.
Detailed Description
The present invention will be further illustrated with reference to the following specific examples.
Example one
The embodiment provides a cylindrical observation room for enhancing parasitic rate of non-volatile allelochemicals, which comprises a circular supporting base 1, a cylindrical cover 2 and air holes 3, and is characterized in that the cylindrical cover 2 is arranged on the supporting base 1, the air holes 3 are formed in the top of the cylindrical cover 2, and a release table 4 and four object tables 5 are arranged on the top of the circular supporting base 1.
The embodiment provides an observation method for enhancing parasitic rate of parasitic wasps by using a non-volatile allelochemicals, which comprises the following steps:
s1: preparation of the experiment: a plurality of tea seedlings, a plurality of leafhoppers and a plurality of female bees were prepared, and a allelochemicals and n-hexane were purchased from a regular supplier.
S2: spawning: selecting leafhoppers and tea seedlings in the S1, selecting a proper greenhouse, putting the tea seedlings into the greenhouse, putting the leafhoppers on tea tips of the tea seedlings to lay eggs, selecting 4 tea tips with eggs on 1 bud and 4 leaves after 24 hours of laying eggs, and taking 2 groups of tea tips with 50 egg laying holes in total;
s3: and (3) regulating the culture environment: the cylindrical observation room is placed in a special laboratory, a fluorescent lamp is prepared and positioned right above the cylindrical observation room, light can be uniformly irradiated into the cylindrical observation room, the temperature of the laboratory is controlled to be 25 ℃ by a temperature and humidity controller, and the relative humidity is 70%.
S4: and (3) concentration adjustment: the taraxacum compound described in S1 was removed and n-hexane was used to prepare a concentration gradient (10)-2g/mL), putting the solvent A to be detected into a first storage cup, and putting n-hexane for comparison into a second storage cup;
s5: point coating: selecting a plurality of identical brush brushes, spot-coating the solvent A to be detected in the first storage beaker in the S4 around the egg-laying holes on one group of the tea tips with the eggs to obtain a treatment group B, and spot-coating the contrast n-hexane in the second storage beaker in the S4 around the egg-laying holes on the other group of the tea tips with the eggs to obtain a treatment group C.
S6: releasing bees: and (3) placing the treated group B and the treated group C treated in the step (S5) on symmetrical object stages 5 in a cylindrical observation chamber, taking 4 female bees in the step (S1) by using a finger-type tube, placing the female bees on a central release table 4 in the cylindrical observation chamber, enabling the tube openings to face upwards, changing the treated group B and the treated group C for 1 hour, repeating the operations for 2 times, changing the positions for 2 times, and taking out the treated group B and the treated group C after the female bees are placed for 12 hours.
S7: culturing: placing the treatment group B and the treatment group C taken out of the S6 into a light incubator for culturing for 5 days to respectively obtain a culture D and a culture E;
s8: counting parasitic eggs: the culture D and the culture E in the S7 were placed under a microscope for observation, and the parasitized eggs of the leafhopper were peeled from the young stem of the tea shoot to count the parasitized rate.
In this embodiment, the material of cylinder type observation room is made for colorless organic glass, the diameter of cylinder type observation room is 80cm, fluorescent lamp in S3 is the 40W fluorescent lamp, the atmospheric control ware model in S3 is TDK0302, microscopical model in S8 is RW0850, the tea age of the tea seedling in S1 is the same and is 3 years old, and the growth vigor of tea seedling is even unanimous, and the clonal potted tea seedling that does not receive the disease, the worm infringement carries out the rich water management according to same standard, and the leafhopper all gains from the reproduction of growing generations in the warmhouse booth, and the female bee that the egg of leafhopper was used as the host in the warmhouse booth, carries out the female bee that the reproduction gained of growing generations, and the female bee is the female bee of 24 hours that emerges, other infectious compound n-hexane in S1 has the non-volatility, can be dissolved in organic solvent n-hexane.
Example two
The embodiment provides a cylindrical observation room for enhancing parasitic rate of non-volatile allelochemicals, which comprises a circular supporting base 1, a cylindrical cover 2 and air holes 3, and is characterized in that the cylindrical cover 2 is arranged on the supporting base 1, the air holes 3 are formed in the top of the cylindrical cover 2, and a release table 4 and four object tables 5 are arranged on the top of the circular supporting base 1.
The embodiment provides an observation method for enhancing parasitic rate of parasitic wasps by using a non-volatile allelochemicals, which comprises the following steps:
s1: preparation of the experiment: a plurality of tea seedlings, a plurality of leafhoppers and a plurality of female bees were prepared, and a allelochemicals and n-hexane were purchased from a regular supplier.
S2: spawning: selecting leafhoppers and tea seedlings in the S1, selecting a proper greenhouse, putting the tea seedlings into the greenhouse, putting the leafhoppers on tea tips of the tea seedlings to lay eggs, selecting 4 tea tips with eggs on 1 bud and 4 leaves after 24 hours of laying eggs, and taking 2 groups of tea tips with 50 egg laying holes in total;
s3: and (3) regulating the culture environment: the cylindrical observation room is placed in a special laboratory, a fluorescent lamp is prepared and positioned right above the cylindrical observation room, light can be uniformly irradiated into the cylindrical observation room, the temperature of the laboratory is controlled to be 25 ℃ by a temperature and humidity controller, and the relative humidity is 70%.
S4: and (3) concentration adjustment: the taraxacum compound described in S1 was removed and n-hexane was used to prepare a concentration gradient (10)-4g/mL), putting the solvent A to be detected into a first storage cup, and putting n-hexane for comparison into a second storage cup;
s5: point coating: selecting a plurality of identical brush brushes, spot-coating the solvent A to be detected in the first storage beaker in the S4 around the egg-laying holes on one group of the tea tips with the eggs to obtain a treatment group B, and spot-coating the contrast n-hexane in the second storage beaker in the S4 around the egg-laying holes on the other group of the tea tips with the eggs to obtain a treatment group C.
S6: releasing bees: and (3) placing the treated group B and the treated group C treated in the step (S5) on symmetrical object stages 5 in a cylindrical observation chamber, taking 4 female bees in the step (S1) by using a finger-type tube, placing the female bees on a central release table 4 in the cylindrical observation chamber, enabling the tube openings to face upwards, changing the treated group B and the treated group C for 1 hour, repeating the operations for 2 times, changing the positions for 2 times, and taking out the treated group B and the treated group C after the female bees are placed for 12 hours.
S7: culturing: placing the treatment group B and the treatment group C taken out of the S6 into a light incubator for culturing for 5 days to respectively obtain a culture D and a culture E;
s8: counting parasitic eggs: the culture D and the culture E in the S7 were placed under a microscope for observation, and the parasitized eggs of the leafhopper were peeled from the young stem of the tea shoot to count the parasitized rate.
In this embodiment, the material of cylinder type observation room is made for colorless organic glass, the diameter of cylinder type observation room is 80cm, fluorescent lamp in S3 is the 40W fluorescent lamp, the atmospheric control ware model in S3 is TDK0302, microscopical model in S8 is RW0850, the tea age of the tea seedling in S1 is the same and is 3 years old, and the growth vigor of tea seedling is even unanimous, and the clonal potted tea seedling that does not receive the disease, the worm infringement carries out the rich water management according to same standard, and the leafhopper all gains from the reproduction of growing generations in the warmhouse booth, and the female bee that the egg of leafhopper was used as the host in the warmhouse booth, carries out the female bee that the reproduction gained of growing generations, and the female bee is the female bee of 24 hours that emerges, other infectious compound n-hexane in S1 has the non-volatility, can be dissolved in organic solvent n-hexane.
EXAMPLE III
The embodiment provides a cylindrical observation room for enhancing parasitic rate of non-volatile allelochemicals, which comprises a circular supporting base 1, a cylindrical cover 2 and air holes 3, and is characterized in that the cylindrical cover 2 is arranged on the supporting base 1, the air holes 3 are formed in the top of the cylindrical cover 2, and a release table 4 and four object tables 5 are arranged on the top of the circular supporting base 1.
The embodiment provides an observation method for enhancing parasitic rate of parasitic wasps by using a non-volatile allelochemicals, which comprises the following steps:
s1: preparation of the experiment: a plurality of tea seedlings, a plurality of leafhoppers and a plurality of female bees were prepared, and a allelochemicals and n-hexane were purchased from a regular supplier.
S2: spawning: selecting leafhoppers and tea seedlings in the S1, selecting a proper greenhouse, putting the tea seedlings into the greenhouse, putting the leafhoppers on tea tips of the tea seedlings to lay eggs, selecting 4 tea tips with eggs on 1 bud and 4 leaves after 24 hours of laying eggs, and taking 2 groups of tea tips with 50 egg laying holes in total;
s3: and (3) regulating the culture environment: the cylindrical observation room is placed in a special laboratory, a fluorescent lamp is prepared and positioned right above the cylindrical observation room, light can be uniformly irradiated into the cylindrical observation room, the temperature of the laboratory is controlled to be 25 ℃ by a temperature and humidity controller, and the relative humidity is 70%.
S4: and (3) concentration adjustment: the taraxacum compound described in S1 was removed and n-hexane was used to prepare a concentration gradient (10)-6g/mL), putting the solvent A to be detected into a first storage cup, and putting undiluted n-hexane into a second storage cup;
s5: point coating: selecting a plurality of identical brush brushes, spot-coating the solvent A to be detected in the first storage beaker in the S4 around the egg-laying holes on one group of the tea tips with the eggs to obtain a treatment group B, and spot-coating the contrast n-hexane in the second storage beaker in the S4 around the egg-laying holes on the other group of the tea tips with the eggs to obtain a treatment group C.
S6: releasing bees: and (3) placing the treated group B and the treated group C treated in the step (S5) on symmetrical object stages 5 in a cylindrical observation chamber, taking 4 female bees in the step (S1) by using a finger-type tube, placing the female bees on a central release table 4 in the cylindrical observation chamber, enabling the tube openings to face upwards, changing the treated group B and the treated group C for 1 hour, repeating the operations for 2 times, changing the positions for 2 times, and taking out the treated group B and the treated group C after the female bees are placed for 12 hours.
S7: culturing: placing the treatment group B and the treatment group C taken out of the S6 into a light incubator for culturing for 5 days to respectively obtain a culture D and a culture E;
s8: counting parasitic eggs: the culture D and the culture E in the S7 were placed under a microscope for observation, and the parasitized eggs of the leafhopper were peeled from the young stem of the tea shoot to count the parasitized rate.
In this embodiment, the material of cylinder type observation room is made for colorless organic glass, the diameter of cylinder type observation room is 80cm, fluorescent lamp in S3 is the 40W fluorescent lamp, the atmospheric control ware model in S3 is TDK0302, microscopical model in S8 is RW0850, the tea age of the tea seedling in S1 is the same and is 3 years old, and the growth vigor of tea seedling is even unanimous, and the clonal potted tea seedling that does not receive the disease, the worm infringement carries out the rich water management according to same standard, and the leafhopper all gains from the reproduction of growing generations in the warmhouse booth, and the female bee that the egg of leafhopper was used as the host in the warmhouse booth, carries out the female bee that the reproduction gained in the generations, and the female bee is the female bee of 24 hours that emerges, other infectious compound n-hexane in S1 has the non-volatility, can be dissolved in organic solvent n-hexane.
It should be noted that the apparatus structure and the attached drawings of the present invention mainly describe the principle of the present invention, and the specific connection structure of the circular support base 1 and the cylindrical cover 2 is not completely described based on the design principle, but the specific connection structure of the circular support base 1 and the cylindrical cover 2 can be clearly known by those skilled in the art on the premise of understanding the inventive principle.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (7)
1. Non-volatility other feels compound reinforcing parasitic bee parasitic rate's cylinder type observation chamber, including round support base (1), cylinder cover (2) and bleeder vent (3), its characterized in that, be equipped with cylinder cover (2) on support base (1), bleeder vent (3) have been seted up at the top of cylinder cover (2), and the top of round support base (1) is equipped with release platform (4) and four objective tables (5).
2. The observation method for enhancing the parasitic rate of the parasitic wasps by using the non-volatile allelochemicals is characterized by comprising the following steps of:
S1: preparation of the experiment: a plurality of tea seedlings, a plurality of leafhoppers and a plurality of female bees were prepared, and a allelochemicals and n-hexane were purchased from a regular supplier.
S2: spawning: selecting leafhoppers and tea seedlings in the S1, selecting a proper greenhouse, putting the tea seedlings into the greenhouse, putting the leafhoppers on tea tips of the tea seedlings to lay eggs, selecting 3-5 branches of 1 bud and 4 leaf tea tips with eggs after 24 hours of laying eggs, and taking 2 groups of tea tips with 50 egg laying holes in total;
s3: and (3) regulating the culture environment: the cylindrical observation room is placed in a special laboratory, a fluorescent lamp is prepared and positioned right above the cylindrical observation room, light can be uniformly irradiated into the cylindrical observation room, the temperature of the laboratory is controlled to be 24-26 ℃ by a temperature and humidity controller, and the relative humidity is 60% -80%.
S4: and (3) concentration adjustment: the taraxacum compound described in S1 was removed and n-hexane was used to prepare a concentration gradient (10)-2g/mL、10-4g/mL、10-6g/mL), putting the solvent A to be detected into a first storage cup, and putting n-hexane for comparison into a second storage cup;
s5: point coating: selecting a plurality of identical brush brushes, spot-coating the solvent A to be detected in the first storage beaker in the S4 around the egg-laying holes on one group of the tea tips with the eggs to obtain a treatment group B, and spot-coating the contrast n-hexane in the second storage beaker in the S4 around the egg-laying holes on the other group of the tea tips with the eggs to obtain a treatment group C.
S6: releasing bees: and (3) placing the treated group B and the treated group C treated in the step (S5) on symmetrical object stages (5) in a cylindrical observation chamber, taking 4 female bees in the step (S1) by using a finger-shaped pipe, placing the female bees on a central release table (4) in the cylindrical observation chamber, enabling the pipe orifices to face upwards, waiting for 1 hour for exchanging the treated group B and the treated group C, repeating the operation for 2 times, exchanging the positions for 2 times, and taking out the treated group B and the treated group C after the female bees are placed for 12 hours.
S7: culturing: placing the treatment group B and the treatment group C taken out of the S6 into a light incubator for culturing for 5 days to respectively obtain a culture D and a culture E;
s8: counting parasitic eggs: the culture D and the culture E in the S7 were placed under a microscope for observation, and the parasitized eggs of the leafhopper were peeled from the young stem of the tea shoot to count the parasitized rate.
3. The cylindrical observation chamber for enhancing parasitic infestation of non-volatile allelochemicals as claimed in claim 1, wherein the cylindrical observation chamber is made of colorless organic glass, and the diameter of the cylindrical observation chamber is 40-80 cm.
4. The method of claim 2, wherein the fluorescent lamp in S3 is a 40W fluorescent lamp, and the temperature and humidity controller in S3 is TDK 0302.
5. The method of claim 2, wherein the microscope at S8 is model RW 0850.
6. The method for observing the parasitic rate of parasitic wasps enhanced by non-volatile allelochemicals according to claim 2, wherein the tea seedlings in S1 are all 3 years old with the same age, have uniform growth vigor, are potted in clone tea seedlings not affected by diseases and insects, are subjected to fertilizer and water management according to the same standard, leafhoppers are all obtained from the greenhouse for generative propagation, female bees are all obtained from the greenhouse for generative propagation by using eggs of leafhoppers as hosts, and are all female bees which emerge for 24 hours.
7. The method of claim 2, wherein said allelochemicals in S1 are non-volatile and soluble in organic solvents such as n-hexane.
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