CN1179639C - Technology for industrially culturing trichogramma with gelechiid ova - Google Patents
Technology for industrially culturing trichogramma with gelechiid ova Download PDFInfo
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- CN1179639C CN1179639C CNB021293449A CN02129344A CN1179639C CN 1179639 C CN1179639 C CN 1179639C CN B021293449 A CNB021293449 A CN B021293449A CN 02129344 A CN02129344 A CN 02129344A CN 1179639 C CN1179639 C CN 1179639C
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- gelechiid
- ovum
- trichogramma
- feed
- ova
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Abstract
The present invention provides a technology for industrially providing gelechiid ova and breeding trichogramma in a large scale. The technology uses gelechiid granule ova as hosts, automatically collects moths in a hopper type via light-shielding vertical breeding, collects multiple generations of moths by once insect taking, collects the ova and removes dust via an autorotation apparatus, removes impurities via air streams, continuously breeds the trichogramma with scattered ova, combines short-term trichogramma storage with long-term trichogramma storage and annually breeds the trichogramma in the factory mode. The present invention can breed stray field granule-ovum trichogramma species by the granule ova so as to widen the species of applied trichogramma, and realize annual trichogramma breeding which helps to fully utilize personnel and equipment and to accumulate lots of trichogramma species.
Description
Technical field
The present invention relates to a kind of technology of cultivating oophagous trichogrammae, particularly use technology for industrially culturing trichogramma with gelechiid ova, belong to the biological control technical field of pest.
Background technology
In the prior art, do vector at the large-scale ovum of the main use of production breeding of China oophagous trichogrammae, its shortcoming is to prevent that the agricultural pests effect is low, and range of application is narrow.The technology of using ovule to do vector is also arranged in recent years, is the patent application of CN1253723A as patent publication No., but the feed of selecting for use is subjected to influence that ambient temperature and humidity changes too greatly; Add water behind the feed disinfection and easily cause secondary pollution; Can not realize once connecing worm and withhold moth more, the collection rate and the cleannes of gelechiid ova are low; Because the structure of numerous beehive causes and can not improve the uniformity and the unit space bee yield that spreads ovum, numerous honeybee quality is low.
Summary of the invention
The objective of the invention is to overcome above deficiency, a kind of technology for industrially culturing trichogramma with gelechiid ova of using be provided, comprise following technical process:
(1) feed is selected: selecting the barley of band Ying shell for use is feed, and utilizing has shell barley self performance of keeping humidity to reduce the influence that feed is subjected to the variation of ambient temperature and humidity.
(2) sterilizing pesticide: barley is eluriated the screen frame formula feed frame (two sides is the wooden frame of stainless (steel) wire) of packing into after the impurity elimination in disinfection cabinet, heat, temperature remain on 85～110 ℃ 60～90 minutes, kill insect and worm's ovum in the feed, take out back barley water content and remain on 14～20%.
(3) connect ovum parasitism and larva raising: the ratio of gelechiid ova in 1: 1000 inserted in the feed that disinfects in the larva raising chamber, feed thickness 3～4cm, the receptacle temperature keeps 28 ± 2 ℃, relative moisture keeps 85 ± 5%, unglazed photograph, for preventing feed mold, receptacle was opened the uviol lamp sterilization weekly 2 times each 25～35 minutes.Connect 3 weeks of ovum, the feed that will contain gelechiid is put into the imago breeding frame, changes the adult collecting box in the lump over to and prepares to collect adult.
(4) raising of adult and collection: in the imago breeding chamber, temperature keeps 28 ± 2 ℃, and relative moisture keeps 80 ± 5%, and unglazed photograph, feed be from changing the adult collecting box over to eliminating 3 months, 3 generations of intermediate collection adult; The adult collecting box is a lower hopper, and the vertical slot in top inserts the imago breeding frame, utilizes gelechiid adult geotropism to collect adult automatically, receive to change gelechiid every day 1～2 time, changes the gelechiid adult over to the adult cage of laying eggs.
(5) collection of ovum and cleaning: at the gelechiid laying coop, temperature keeps 24 ± 2 ℃, relative moisture keeps 85 ± 5%, unglazed photograph, after the gelechiid adult was poured the cage of laying eggs into, the cage of will laying eggs was placed on the ovum collecting machine, and gathering machine drove the cage rotation 10 minutes of laying eggs automatically in per 2 hours, brush the gelechiid ova on the cage that falls to laying eggs, the air extractor of opening simultaneously on the gathering machine is removed gelechiid scale and fine dust; Per 24 hours, the gelechiid ova that will be dropped on the baffle plate by the special messenger collected, with the clear egg apparatus of air-flowing type and sub-sieve cleaning gelechiid ova and store classifiedly.
(6) depositing of ovum: the ovum of depositing numerous honeybee ovum respectively and being used to breed gelechiid with the refrigerator of 4 ± 1 ℃ and 10 ± 1 ℃.
(7) diffusing parasitic bee: in numerous honeybee workshop, temperature keeps 26 ± 2 ℃, and relative moisture keeps 75 ± 5%, 14: 10 Light To Dark Ratios, i.e. illumination 14 hours every days, 10 hours dark.Plant honeybee and connect ovum than planting a honeybee by breeding in 1: 15,1: 8～10 propagation production are used honeybee, and laying off eggs is sprinkling upon on the aluminium-plastic panel that adds mist, every plate spreads ovum 10～13g, uviol lamp irradiation down used after killing the gelechiid embryo in 30 minutes, and numerous beehive is 13 layers of numerous beehive of structure of 3 lattice, and the width ratio of every layer 3 lattice is 3: 1: 3.Single case day numerous honeybee 450～5,000,000, connect the honeybee time between 24～48 hours, take out the mixed pallet that changes over to of parasitic good gelechiid ova after 48 hours and grow after optimum period in numerous honeybee chamber and make the ovum card.
(8) storage honeybee: short is to deposit under 10 ± 1 ℃ of conditions, changing refrigerator from the placement in 20 ℃ incubator of taking-up back, numerous honeybee chamber over to after half a day before egg parasitoid is deposited deposits, deposit a few days ago to stir every day and store vessel once, prevent the egg parasitoid caking, short-term is deposited with in 10 days, stores the quality that use after 15～20 days will detect honeybee; Long term storage is to deposit under 3 ± 1 ℃ of conditions, adopts and induces the oophagous trichogrammae diapause, and the way of refrigeration diapause honeybee can be stored 3～6 months, uses later in 4～6 months in refrigeration.
Effect of the present invention is: selecting the barley with clever shell for use is feed, reduces the influence that feed is changed by ambient temperature and humidity; With the screen frame barley of sterilizing, reduced and added the probability that water causes secondary pollution behind the feed disinfection; Once connecing worm withholds moth more and has improved the raising operating efficiency; Utilize gelechiid self geotropism to collect adult, reduce the consumption that machinery is received moth; The automatic receipts ovum machine of timing working can effectively improve the collection rate and the cleannes of ovum; Can improve cleaning efficiency with clear egg apparatus of air-flowing type and sub-sieve cleaning gelechiid ova; Numerous beehive that adopts and use aluminium-plastic panel replace paperboard holder ovum, improve the uniformity and the unit space bee yield that spread ovum; Use the granule ovum can breed parasitic field breeding bee of parvule-egg, widen and use the oophagous trichogrammae kind, and the realization anniversary numerous honeybee be beneficial to the personnel of making full use of, equipment, be convenient to accumulate in a large number the honeybee kind.
Embodiment
Further specify below in conjunction with embodiment:
A kind of technology for industrially culturing trichogramma with gelechiid ova of using, comprise following technical process: selecting the barley 135g of band Ying shell for use is feed, barley is eluriated the feed frame (two sides is the wooden frame of stainless (steel) wire) of packing into after the impurity elimination in high-temperature disinfection cabinet, be heated to 100 ℃, keep after 60 minutes taking out that barley is cooled off naturally, drying one day, barley water content 15%.Divide 3 batches (every crowd of 50kg, 1 weeks at interval) feed box of quantitatively packing into the barley that disinfects, change the larva raising chamber in the feed thickness 4cm, every box behind the access gelechiid ova 4.5ml (about 2.5g) over to and cultivate with every box 2.5kg.Connecing worm will be with the worm feed to change the imago breeding frame over to change the adult collecting box in the lump over to and prepare to collect adult after 3 weeks.Each imago breeding frame can hold the 7.5kg feed, and single adult collecting box can hold 18 frames.Connect worm from first and begin to have the gelechiid adult eclosion after the 28th day, average day collection gelechiid adult 88.9g in later 2 months.Pack into the cage of laying eggs of the adult of collecting is placed on the gelechiid ova gathering machine, temperature remains on 24 ℃, and relative moisture keeps 85%, collects gelechiid ova under the no optical condition, the gelechiid ova that arrive with clear egg apparatus of air-flowing type and sub-sieve clean catch every day, per day receipts ovum 12.2g.Deposit standby by producing the refrigerator that needs respectively 3 ℃ and 10 ℃ the gelechiid ova collected.The 3rd batch of adult eclosion peak adds up to collection gelechiid adult 7115g, gelechiid ova 976g later after 2 months.To raise frame and take out and to be heated to 70 ℃ and to kill residual worm and sorted remaining feed in 1 hour, remaining 4.5kg feed reclaims and uses.
Gelechiid ova evenly is sprinkling upon on the aluminium-plastic panel that adds mist, and irradiation changed in numerous beehive after killing the gelechiid embryo in 30 minutes under uviol lamp, inserted out the kind honeybee of honeybee about 30% in the ratio of honeybee ovum than 1: 10.At 26 ℃, relative moisture keeps under 75%, 14: the 10 Light To Dark Ratio conditions, i.e. illumination 14 hours every days, 10 hours dark.Taking out the mixed pallet that changes over to of parasitic good gelechiid ova after 24 hours grows in numerous honeybee chamber and makes the ovum card after the optimum period or take other measure to handle the back and store.
Be sprinkling upon on the paperboard of grid-like glue face after even with 5 days egg parasitoid is mixed in 4 days growing, every card contains 100 lesser calories, and every lesser calorie single deck tape-recorder goes out 1200 of honeybees.
Claims (1)
1, a kind of technology for industrially culturing trichogramma with gelechiid ova of using is characterized in that comprising following technical process:
(1) feed is selected: selecting the barley of band Ying shell for use is feed, reduces the influence that feed is subjected to the variation of ambient temperature and humidity;
(2) sterilizing pesticide: barley is eluriated the screen frame formula feed frame of packing into after the impurity elimination as feed, in disinfection cabinet, heats, temperature remain on 85～110 ℃ 60～90 minutes, kill insect and worm's ovum in the feed, take out back barley water content and remain on 14～20%;
(3) connect ovum parasitism and larva raising: the ratio of gelechiid ova in 1: 1000 inserted in the feed that disinfects in the larva raising chamber, feed thickness 3～4cm, the receptacle temperature remains on 28 ± 2 ℃, relative moisture remains on 85 ± 5%, and unglazed photograph is for preventing feed mold, receptacle was opened the uviol lamp sterilization weekly 2 times each 25～35 minutes, connect 3 weeks of ovum, the feed that will contain gelechiid is put into the imago breeding frame, changes the adult collecting box in the lump over to and prepares to collect adult;
(4) raising of adult and collection: in the imago breeding chamber, temperature keeps 28 ± 2 ℃, relative moisture keeps 80 ± 5%, unglazed photograph, feed be from changing the adult collecting box over to eliminating 3 months, 3 generations of intermediate collection adult, the adult collecting box is that lower hopper, top are that vertical slot inserts the imago breeding frame, utilize gelechiid adult geotropism to collect adult automatically, receive to change gelechiid every day 1～2 time, change the gelechiid adult over to the adult cage of laying eggs;
(5) collection of ovum and cleaning: at the gelechiid laying coop, temperature keeps 24 ± 2 ℃, relative moisture keeps 85 ± 5%, unglazed photograph, after the gelechiid adult is poured the cage of laying eggs into, the cage of will laying eggs is placed on the ovum collecting machine, gathering machine drove the cage rotation 10 minutes of laying eggs automatically in per 2 hours, brush the gelechiid ova on the cage that falls to laying eggs, open the air extractor on the gathering machine simultaneously, gelechiid scale and fine dust are removed per 24 hours, the gelechiid ova that will be dropped on the baffle plate by the special messenger collects, with the clear egg apparatus of air-flowing type and sub-sieve cleaning gelechiid ova and store classifiedly;
(6) depositing of ovum: the ovum of depositing numerous honeybee ovum respectively and being used to breed gelechiid with the refrigerator of 4 ± 1 ℃ and 10 ± 1 ℃;
(7) diffusing parasitic bee: in numerous honeybee workshop, temperature keeps 26 ± 2 ℃, relative moisture keeps 75 ± 5%, illumination 14 hours every days, 10 hours dark, plant honeybee and connect ovum than planting a honeybee by breeding in 1: 15,1: 8～10 propagation production are used honeybee, and laying off eggs is sprinkling upon on the aluminium-plastic panel that adds mist, and every plate spreads ovum 10～13g, uviol lamp irradiation down used after killing the gelechiid embryo in 30 minutes, numerous beehive is 13 layers of numerous beehive of 3 lattice, and the width ratio of every layer 3 lattice is 3: 1: 3, single case day numerous honeybee 450～5,000,000, connect the honeybee time between 24～48 hours, take out the mixed pallet that changes over to of parasitic good gelechiid ova after 48 hours and grow after optimum period in numerous honeybee chamber and make the ovum card;
(8) storage honeybee: short is to deposit under 10 ± 1 ℃ of conditions, egg parasitoid is deposited preceding after the taking-up of numerous honeybee chamber, in 20 ℃ incubator, place and change refrigerator over to after half a day and deposit, deposit a few days ago to stir every day and store vessel once, prevent the egg parasitoid caking, short-term is deposited with in 10 days, store use after 15～20 days and will detect the quality of honeybee, long term storage is to deposit under 3 ± 1 ℃ of conditions, the oophagous trichogrammae diapause is induced in employing, the way of refrigeration diapause honeybee can be stored 3～6 months, used later in 4～6 months in refrigeration.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021293449A CN1179639C (en) | 2002-09-04 | 2002-09-04 | Technology for industrially culturing trichogramma with gelechiid ova |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021293449A CN1179639C (en) | 2002-09-04 | 2002-09-04 | Technology for industrially culturing trichogramma with gelechiid ova |
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| CN1397159A CN1397159A (en) | 2003-02-19 |
| CN1179639C true CN1179639C (en) | 2004-12-15 |
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| CNB021293449A Expired - Lifetime CN1179639C (en) | 2002-09-04 | 2002-09-04 | Technology for industrially culturing trichogramma with gelechiid ova |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101933494A (en) * | 2010-08-27 | 2011-01-05 | 广东省昆虫研究所 | Moth-egg collecting machine |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101326902B (en) * | 2008-07-04 | 2011-05-04 | 衡水田益生防有限责任公司 | Method for inducing diapause of Trichogramma ostriniae by constant temperature and storage method |
| CN101356906B (en) * | 2008-09-16 | 2010-12-15 | 广东省昆虫研究所 | Artificial propagation method and apparatus of egg parasitoids |
| CN102106319B (en) * | 2011-01-18 | 2012-06-27 | 西北农林科技大学 | Method for propagating trichogramma pintoi voegele indoors by grapholitha molesta busck egg |
| CN104255660A (en) * | 2014-09-01 | 2015-01-07 | 吉林农业大学 | Method for alternately storing trichogramma at intermediate and low temperature in two steps |
| CN106614396A (en) * | 2016-12-02 | 2017-05-10 | 中国农业科学院植物保护研究所 | Method for feeding sitotroga cerealella by utilizing unhusked rice |
| CN108450419A (en) * | 2018-02-26 | 2018-08-28 | 吉林省农业科学院 | A kind of Trichogramma chilonis Diapause inducement method |
| CN111011313B (en) * | 2019-12-30 | 2022-04-19 | 中国医学科学院药用植物研究所云南分所 | Method for improving female proportion of trichogramma offspring and application thereof |
| CN111685083A (en) * | 2020-07-02 | 2020-09-22 | 吉林省农业科学院 | Method for raising wheat moths by using wheat |
| CN116171941A (en) * | 2023-03-08 | 2023-05-30 | 福建省农业科学院水稻研究所 | Application of wheat seedlings in cold storage of trichogramma longsynanthassemblies and cold storage method |
-
2002
- 2002-09-04 CN CNB021293449A patent/CN1179639C/en not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101933494A (en) * | 2010-08-27 | 2011-01-05 | 广东省昆虫研究所 | Moth-egg collecting machine |
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| CN1397159A (en) | 2003-02-19 |
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Owner name: DRY FARMING AGRICULTURE INST., HEBEI PROV. ACADEMY Free format text: FORMER OWNER: TIANYI BIOLOGIC PREVENTION CO LTD, HENGSHUI Effective date: 20140917 Owner name: HENGSHUI AGRICULTURAL SCIENCE RESEARCH INSTITUTE Effective date: 20140917 |
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Effective date of registration: 20140917 Address after: Hengshui City, Hebei province Shengli road 053000 Taocheng District No. 1966 Patentee after: DRY-LAND FARMING INSTITUTE OF HEBEI ACADEMY OF AGRICULTURAL AND FORESTRY SCIENCES Patentee after: Hengshui Academy of Agricultural Sciences Address before: 053000 No. 122 South Gate Street, Hebei, Hengshui Patentee before: Hengshui Tianyi Biocontrol Co.,Ltd. |
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Application publication date: 20030219 Assignee: Hengshui bee Biotechnology Co.,Ltd. Assignor: Dry-Land Farming Institute of Hebei Academy of Agricultural and Forestry Sciences Contract record no.: 2015130000117 Denomination of invention: Technology for industrially culturing trichogramma with gelechiid ova Granted publication date: 20041215 License type: Exclusive License Record date: 20150730 |
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