CN111406713A - Method for breeding parasitic wasps on large scale - Google Patents

Method for breeding parasitic wasps on large scale Download PDF

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CN111406713A
CN111406713A CN201910015460.4A CN201910015460A CN111406713A CN 111406713 A CN111406713 A CN 111406713A CN 201910015460 A CN201910015460 A CN 201910015460A CN 111406713 A CN111406713 A CN 111406713A
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aphids
wheat
stiff
plants
aphid
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CN111406713B (en
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张彦周
李宏亮
张旭
秦耀光
欧阳竹
孙志刚
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Institute of Zoology of CAS
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates

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Abstract

The invention provides a method for breeding parasitic wasps on a large scale, which comprises the following steps: 1) host plants were grown in regions that satisfied the following conditions: the annual average precipitation is 1000-1200 mm, the annual average temperature is 15-17 ℃, and the water is located in an area between 31-33 degrees of north latitude; 2) enabling the aphid occurrence amount of the host plant to exceed 300/hundred plants so as to breed the aphidiidae; 3) the parts of the host plant carrying the stiff aphids are collected from 1 to 15 days in 5 months each year. The invention provides a method for controlling agricultural pests, which comprises the step of uniformly broadcasting wheat straws, leaves or ears with stiff aphids in a ratio of 1-2 kilo mu per mu in a crop field needing to release parasitic wasps. The method can be popularized to the purpose of preventing and controlling pests by breeding other aphids and parasitic wasps in the south and north directions, is used for biological prevention and control of other pests, and has stable effect.

Description

Method for breeding parasitic wasps on large scale
Technical Field
The invention belongs to the technical field of biological control of agricultural pests, relates to a breeding production method of parasitic wasps, in particular to a method for breeding parasitic wasps in a large scale, and also relates to a method for controlling pests by using parasitic wasps
Background
At present, chemical control is mainly used for controlling aphids in a field, so that the drug resistance of wheat aphids is enhanced year by year; the quantity of the natural enemies in the field is continuously reduced, so that the natural control capability of the natural enemies is weakened, a large amount of aphids are bred, and the damage is aggravated. In addition, pesticide residue, environmental pollution and the like generated by chemical control can cause toxicity to people and livestock. Under the background, it is important to control the damage of aphids by using biological control technology which takes natural enemies as main means. Many successful cases of biological control by using parasitic wasps exist at home and abroad.
The method for controlling the pests by using the parasitic natural enemy insects is not only safe to the environment, but also an effective means for continuously controlling the harm of the pests. In biological control of pests, there are three methods for the application of natural enemies: foreign natural enemies are introduced, the natural enemies are protected and utilized, and the natural enemies are artificially bred in large quantities. The third method is generally used at present.
Aphidiidae is an important parasitic natural enemy of aphids, and has been widely used for preventing and controlling aphids on various crops, the mode of breeding bees (only adopting artificial assisted aphid breeding under the condition that the aphids are few) is mainly adopted to breed bees naturally at the present stage, the mode of releasing stiff aphid seedlings or forming bees is difficult to meet the requirements of popularization and application, stiff aphid products which can be refrigerated for a long term and are convenient to transport and release are urgently needed in production, and the efficient breeding of the stiff aphids which are orderly developed is a prerequisite for producing the stiff aphid products. Different aphidiidae have parasitism specificity and preference for different aphids. At present, technicians in the field use stiff aphid seedlings, stiff aphid leaves or adult bees for field release in order to meet the requirements of local farmlands for breeding aphidiidae, the transportation efficiency is low, a large number of aphidiidae are easy to die, and the radiation radius is generally less than 50 kilometers.
For mass breeding of parasitic wasps, it is necessary to provide an environment suitable for their growth, i.e. the factors that determine their growth, such as temperature, humidity, and light. In order to obtain a large amount of robust parasitic wasps in time, the relationship among the three must be coordinated, otherwise the robust degree, the sex ratio and the parasitic rate of the parasitic wasps are affected, and a large amount of funds are needed to construct a manual breeding room for raising and breeding the wasps.
China is wide in regions, the climate difference is obvious in the regions of south and north, and the occurrence periods of pests and parasitic natural enemies thereof on the same economic crops planted in different regions are different. However, no precedent exists in the prior art for breeding parasitic wasps on a large scale by utilizing the climate difference.
Disclosure of Invention
Based on the above, the invention aims to provide a method for breeding parasitic wasps on a large scale. The method provided by the invention can greatly increase the population quantity of the natural enemies, is low in cost, is simple and easy to operate, and does not need to artificially raise parasitic wasps for a long time, thereby saving a large amount of manpower, material resources and financial cost.
In one aspect, the present invention provides a method for large-scale breeding of parasitic wasps, comprising the steps of:
1) selecting a breeding area, and breeding host plants (mainly gramineae); wherein the region satisfies the following conditions:
the annual average precipitation is 1000-1200 mm, the annual average temperature is 15-17 ℃, and the water-cooled cable is positioned in a region between 31-33 degrees north latitude;
preferably, the host plant is strictly controlled and is not sprayed with the pesticide so as to ensure that the wheat aphid and the natural enemy thereof can be naturally bred;
more preferably, the host plant is wheat.
2) Enabling the aphid occurrence amount of the host plant to exceed 300/hundred plants so as to breed the aphidiidae;
3) the parts of the host plant carrying the stiff aphids are collected from 1 to 15 days in 5 months each year.
Preferably, the parasitic wasps are mainly avenae Aphidius avenae (aphidia avenae) and Aphidius gifuensis (Aphidius gifuensis) and the like.
Preferably, in step 2), the aphids of the sinonovacula graminifolia (Rhopalosiphum padi) and the triarrhena sacchariflora (Sitobion miscanthhi) are helped to migrate to the host plant, so that the occurrence of the aphids exceeds 300 heads per hundred plants.
Preferably, in the step 3), taking down the leaves, branches or ears with the stiff aphids, putting the leaves, branches or ears into a cage net, putting the cage net into a packaging box, and refrigerating at low temperature;
more preferably, in the step 3), under the conditions that the temperature is 20-30 ℃, the illumination is 12-14 h and the relative humidity is 60-80%, the leaves, branches or ears of the host plant with the stiff aphids are taken down, the host plant with the stiff aphids is placed into a cage net, and then the cage net is placed into a packaging box for low-temperature refrigeration.
Preferably, said aphid (bee species) further comprises:
4) transporting the stiff aphids temporary storage cage net filled with the parts of the host plants with the stiff aphids under the condition of keeping ventilation and cool; more preferably, the time of said transporting does not exceed 48 hours;
in another aspect, the invention provides a method of controlling agricultural pests, said method comprising uniformly broadcasting a portion of a host plant carrying aphid stiff in a proportion of 1 to 2 thousand acres per acre in a field of crops where parasitic wasps are to be released.
Preferably, under the conditions that the temperature is 16-34 ℃, the illumination is 12-14 h and the relative humidity is 60-80%, the part of the host plant with the stiff aphids is placed in a crop field needing to control agricultural pests, so that the adult bees are parasitized on the wheat aphids with the aphid plants.
Preferably, the crop field is a region with annual average precipitation of 500-800 mm, annual average temperature of 10-15 ℃ and north latitude of 35-38 degrees.
Preferably, the method further comprises the step of selecting the bee keeping time, wherein the bee keeping time is selected from the early occurrence period (from early May to middle May every year) of local aphids, namely the bee keeping operation can be carried out shortly after the aphids appear;
preferably, the ratio of the number of adult aphidiidae to the number of wheat aphids is 1: 50-300.
Preferably, each mu of stiff aphid can prevent and control insect pests in 1-2 thousand mu of crop fields in the north
In a preferred embodiment, the present invention provides a method for mass breeding parasitic wasps and controlling agricultural pests, the method comprising the steps of:
1) wheat was grown in regions that satisfied the following conditions:
the annual average precipitation is 1000-1200 mm, the annual average temperature is 15-17 ℃, and the water-cooled cable is positioned in a region between 31-33 degrees north latitude; spraying no pesticide during the cultivation period so as to ensure that wheat aphids and natural enemies thereof can be bred naturally;
2) in the growth period of wheat, enabling the sinonovacula graminifolia (Rhopalosiphum padi) and silutipes graminifolia (Sitobion miscatanthi) to be moved to the cultivated wheat in an auxiliary mode, enabling the aphid generation amount to exceed 300 heads per hundred plants, and cultivating aphidiidae;
3) taking down leaves, branches or ears with stiff aphids of the aphidiidae under the conditions of temperature of 20-30 ℃, illumination of 12-14 h and relative humidity of 60-80% every 5 months for 1-15 days every year, loading into a cage net, then placing into a packaging box, and refrigerating at low temperature;
4) transporting the stiff aphids temporary storage cage net filled with the parts of the host plants with the stiff aphids under the condition of keeping ventilation and cool, wherein the transportation process does not exceed 48 hours;
5) placing the part of the host plant with the stiff aphids into a crop field needing to control agricultural pests under the conditions that the temperature is 16-34 ℃, the illumination is 12-14 hours and the relative humidity is 60-80%, wherein the crop field is a region with annual average precipitation of 500-800 mm, the annual average temperature is 10-15 ℃, and the region is located between 35-38 degrees north latitude, so that adult bees are parasitized on wheat aphids with aphid plants; wherein the ratio of the number of the adult aphidiidae to the number of the wheat aphids is 1: 50-300.
Compared with the prior art, the invention has the following advantages:
1) the method of the invention has the advantages of easily-accessible raw materials and low cost.
2) The species of the parasitic wasps of the wheat field aphids can parasitize various wheat aphids;
3) the transportation method is simple and convenient, and a common technician can independently complete the operation;
4) the method can be popularized to the purposes of preventing and controlling pests by breeding other aphids and parasitic wasps in the south and north directions, and is used for biological prevention and control of other pests;
5) the effect is stable, the aphidiidae emerged from the stiff aphids can carry out parasitic action well, and the generated offspring can continue parasitic action, so that the aphidiidae can adapt to the environment of a transplanting place well, and the population quantity is effectively increased, as shown in the embodiment tables 1-4 and the attached figures 1-4 of the invention.
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Embodiments of the invention are described in detail below with reference to the attached drawing figures, wherein:
fig. 1 shows the variation of the number of wheat aphids in the state of coast in 2016, wherein, through dynamic monitoring of the number of wheat aphids in different time periods of the wheat field, it can be seen that the wheat aphids start to enter the wheat field in the first 5 th month, reach the peak in the middle 5 th month to the last, and sharply decrease in the number of wheat aphids from the end of 5 th month to the beginning of 6 th month;
fig. 2 shows the change of the number of myzus persicae produced in Yucheng in 2016, wherein the number of myzus persicae produced in different periods of the wheat field is dynamically monitored, so that the myzus persicae begins to enter the wheat field in the first 5 th month, the peak is reached in the middle 5 th month to the last, and the number of the myzus persicae sharply decreases from the bottom 5 month to the beginning 6 th month;
fig. 3 shows the variation of the number of wheat aphids in the coastal state in 2017, wherein the number of wheat aphids in different time periods of the wheat field is dynamically monitored, so that the wheat aphids start to enter the wheat field in the first 5 th month, reach the peak in the middle to the last 5 th month, and sharply decrease from the bottom of the 5 th month to the beginning of the 6 th month.
Fig. 4 shows the variation of the number of wheat aphids in the coastal areas in 2018, wherein the number of wheat aphids in different time periods of the wheat field is dynamically monitored, so that the wheat aphids start to enter the wheat field in the first 5 th month, reach the peak in the middle to the last 5 th month, and sharply decrease from the bottom of the 5 th month to the beginning of the 6 th month.
Detailed Description
The present invention is described in further detail below with reference to specific embodiments, which are given for the purpose of illustration only and are not intended to limit the scope of the invention.
Example 1 propagation of Aphidius avenae
The equipment and conditions for mass breeding of aphidiidae in the embodiment comprise:
a bee breeding base: jiangsu Suzhou farm courtyard
The aphid storage cage is 100 meshes in the gauze, and is composed of a refrigerated transport vehicle, a special release device and the like.
1) In the test field of Jiangsu Suzhou agricultural academy, wheat is cultivated according to perennial operation; in the period, the spraying is strictly controlled not to be carried out so as to ensure that the wheat aphids and the natural enemies thereof can be naturally bred;
2) during the growth of wheat, enabling the aphids to be more than 300 heads/hundred plants by helping to migrate the sinonovacula avenae (Rhopalosiphum padi) and the silobionmiscarphis yensis to the cultivated wheat so as to cultivate aphidiidae;
3) in the aphid emergence period, 5 months and 1-15 days, under the conditions of temperature of 20-30 ℃, illumination of 12-14 hours and relative humidity of 60-80%, taking down leaves, branches or ears of a host plant with the stiff aphids of the bombesia persicae, putting the host plant into a cage net, putting the cage net into a packaging box, and refrigerating at low temperature.
As a result: breeding the grain aphid of the sinonovacula Rhopalosiphum padi and the silobion miscanthi, and harvesting the parasitic wasps mainly comprising the avenae Aphidius avenae aphitis avenae and the sultap aphidus gifuensis and the like.
Example 2 Pest control
1) Collecting the stiff aphids, and transporting the stiff aphids from Suzhou to Shandong Binzhou and Yuzhou in a 5-15-month-year transportation process), wherein the transportation tool is a refrigerated truck;
2) 5-15 days every year, under the conditions that the temperature is 16-34 ℃, the illumination is 12-14 h and the relative humidity is 60-80%, putting the stiff aphids into a wheat field needing to release parasitic wasps in a large area, and enabling adult wasps to be parasitic on the wheat aphids with the aphid plants; wherein the ratio of the number of the adult aphidiidae to the number of the wheat aphids is 1: 50-300.
The results are shown in tables 1-4 below and FIGS. 1-4.
In the table below, bees are released once in the Shandong Yucheng for one year, and bees are released once in the Shandong Binzhou for three years. Fig. 1 shows the variation of the number of wheat aphids in the state of coast in 2016, wherein, through dynamic monitoring of the number of wheat aphids in different time periods of the wheat field, it can be seen that the wheat aphids start to enter the wheat field in the first 5 th month, reach the peak in the middle 5 th month to the last, and sharply decrease in the number of wheat aphids from the end of 5 th month to the beginning of 6 th month;
fig. 2 shows the change of the number of myzus persicae produced in Yucheng in 2016, wherein the number of myzus persicae produced in different periods of the wheat field is dynamically monitored, so that the myzus persicae begins to enter the wheat field in the first 5 th month, the peak is reached in the middle 5 th month to the last, and the number of the myzus persicae sharply decreases from the bottom 5 month to the beginning 6 th month;
fig. 3 shows the variation of the number of wheat aphids in the coastal state in 2017, wherein the number of wheat aphids in different time periods of the wheat field is dynamically monitored, so that the wheat aphids start to enter the wheat field in the first 5 th month, reach the peak in the middle to the last 5 th month, and sharply decrease from the bottom of the 5 th month to the beginning of the 6 th month.
Fig. 4 shows the variation of the number of wheat aphids in the coastal areas in 2018, wherein the number of wheat aphids in different time periods of the wheat field is dynamically monitored, so that the wheat aphids start to enter the wheat field in the first 5 th month, reach the peak in the middle to the last 5 th month, and sharply decrease from the bottom of the 5 th month to the beginning of the 6 th month.
Table 1: binzhou 2016 hundred plant aphid quantity statistical table
Date Average value of bee group Control group
2016.4.26 0 0
2016.5.1 3 5
2016.5.6 24 27
2016.5.11 59 58
2016.5.16 231 210
2016.5.21 98 291
2016.5.26 33 166
2016.5.31 7 32
2016.6.5 0 0
In the study, 4 samples were selected for bee-keeping, and 1 sample was selected as a control.
Table 2: statistical table of aphid amount of hundred plants in Yucheng 2016 year
Figure BDA0001938891580000061
Figure BDA0001938891580000071
In the study, 4 samples were selected for bee-keeping, and 1 sample was selected as a control.
Table 3: binzhou 2017 hundred plant aphid quantity statistical table
Date Average value of bee group Control group
2017.4.28 0 0
2017.5.3 11 17
2017.5.8 26 34
2017.5.13 48 63
2017.5.18 155 305
2017.5.23 83 358
2017.5.28 18 98
2017.6.2 0 0
2017.6.7 0 0
In the study, 4 samples were selected for bee-keeping, and 1 sample was selected as a control.
Table 4: binzhou 2018 hundred plant aphid quantity statistical table
Figure BDA0001938891580000072
Figure BDA0001938891580000081
In the study, 4 samples were selected for bee-keeping, and 1 sample was selected as a control.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and are not limited. Although the present invention has been described in detail with reference to the embodiments, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted without departing from the spirit and scope of the invention as defined in the appended claims.

Claims (10)

1. A method for large scale breeding of parasitic wasps, the method comprising the steps of:
1) host plants were grown in regions that satisfied the following conditions:
the annual average precipitation is 1000-1200 mm, the annual average temperature is 15-17 ℃, and the water-cooled cable is positioned in a region between 31-33 degrees north latitude;
2) enabling the aphid occurrence amount of the host plant to exceed 300/hundred plants so as to breed the aphidiidae;
3) parts of the host plant carrying the stiff aphids are collected 1 to 15 days in 5 months per year.
2. The method according to claim 1, wherein the parasitic wasp is an avenae (aphidia avenae) and/or a sulzer braconid bee (aphidia gifuensis).
3. The method according to claim 1, wherein in step 2), the aphids of the Sitobion microscopa (Rhopalosiphumpadi) and Aphis saccharina (Sitobion microsanthi) are helped to migrate to the host plant in an amount of more than 300 aphids per hundred plants.
4. The method according to claim 1, wherein in step 3), under the conditions of temperature of 20-30 ℃, illumination of 12-14 h and relative humidity of 60-80%, leaves, branches or ears of the host plant with the stiff aphid are taken off, put into a cage net, put into a packaging box and refrigerated at low temperature.
5. The method of claim 1, wherein the method further comprises: 4) transporting the stiff aphids temporary storage cage net filled with the parts of the host plants with the stiff aphids under the condition of keeping ventilation and cool; preferably, the time of said transporting does not exceed 48 hours.
6. A method of controlling agricultural pests, said method comprising uniformly broadcasting a portion of a host plant with stiff aphids obtained according to the method of any one of claims 1 to 5 in a field of crops where the release of parasitic wasps is desired, at a rate of 1 to 2 thousand acres per acre of control.
7. A method according to claim 6, wherein the portion of the host plant with stiff aphids is placed in a field of crops where control of agricultural pests is desired, at a temperature of 16 to 34 ℃, a light of 12 to 14 hours and a relative humidity of 60 to 80%, such that the adult bees are parasitic on the Myzus avenae with the aphid plant.
8. The method according to claim 6, wherein the crop field is a region with an annual average precipitation of 500-800 mm, an annual average temperature of 10-15 ℃ and between 35-38 ° north latitude.
9. The method of claim 6, wherein 1-2 thousand acres of the pest control of a field of crop plants in the north is achieved per acre of stiff aphid.
10. A method for mass breeding parasitic wasps and controlling agricultural pests, the method comprising the steps of:
1) wheat was grown in regions that satisfied the following conditions:
the annual average precipitation is 1000-1200 mm, the annual average temperature is 15-17 ℃, and the water-cooled cable is positioned in a region between 31-33 degrees north latitude; during the cultivation period, no pesticide is sprayed to ensure the natural breeding of wheat aphids and natural enemies thereof;
2) during the growth of wheat, the sinonovacula graminifolia (Rhopalosiphum padi) and the silphium saccharifolium (Sitobion microsanthhi) are transferred to the cultivated wheat in an auxiliary mode, so that the aphid generation amount exceeds 300 heads/hundred plants, and the aphidiidae is cultivated;
3) taking down leaves, branches or ears with stiff aphids of the aphidiidae under the conditions of temperature of 20-30 ℃, illumination of 12-14 h and relative humidity of 60-80% every 5 months for 1-15 days every year, loading into a cage net, then placing into a packaging box, and refrigerating at low temperature;
4) transporting the stiff aphids temporary storage cage net filled with the parts of the host plants with the stiff aphids under the condition of keeping ventilation and cool, wherein the transportation process does not exceed 48 hours;
5) placing the part of the host plant with the stiff aphids into a crop field needing to control agricultural pests under the conditions that the temperature is 16-34 ℃, the illumination is 12-14 hours and the relative humidity is 60-80%, wherein the crop field is a region with annual average precipitation of 500-800 mm, the annual average temperature is 10-15 ℃, and the region is located between 35-38 degrees north latitude, so that adult bees are parasitized on wheat aphids with aphid plants; wherein the ratio of the number of the adult aphidiidae to the number of the wheat aphids is 1: 50-300.
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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103478083A (en) * 2013-09-29 2014-01-01 任伟 Large-scale breeding method for breeding mummified aphids and adult bees of aphidiusgifuensis by yellow-green myzuspersicae
CN104938262A (en) * 2015-06-29 2015-09-30 宁波市农业科学研究院 Aphid biological control method with barley and Sitobion avenae as storage carriers
CN106689066A (en) * 2016-12-08 2017-05-24 辽宁省烟草公司朝阳市公司 Method for quickly breeding aphidius gifuensis

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103478083A (en) * 2013-09-29 2014-01-01 任伟 Large-scale breeding method for breeding mummified aphids and adult bees of aphidiusgifuensis by yellow-green myzuspersicae
CN104938262A (en) * 2015-06-29 2015-09-30 宁波市农业科学研究院 Aphid biological control method with barley and Sitobion avenae as storage carriers
CN106689066A (en) * 2016-12-08 2017-05-24 辽宁省烟草公司朝阳市公司 Method for quickly breeding aphidius gifuensis

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