CN108419758A - A kind of efficient numerous bee method for improving aphid parasite deadlock aphid and forming equilibrium degree - Google Patents
A kind of efficient numerous bee method for improving aphid parasite deadlock aphid and forming equilibrium degree Download PDFInfo
- Publication number
- CN108419758A CN108419758A CN201810088840.6A CN201810088840A CN108419758A CN 108419758 A CN108419758 A CN 108419758A CN 201810088840 A CN201810088840 A CN 201810088840A CN 108419758 A CN108419758 A CN 108419758A
- Authority
- CN
- China
- Prior art keywords
- aphid
- bee
- numerous
- cigarette
- stiff
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241001124076 Aphididae Species 0.000 title claims abstract description 276
- 238000000034 method Methods 0.000 title claims abstract description 50
- 244000045947 parasite Species 0.000 title claims abstract description 27
- 235000019504 cigarettes Nutrition 0.000 claims abstract description 108
- 241000208125 Nicotiana Species 0.000 claims abstract description 30
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims abstract description 30
- 230000003071 parasitic effect Effects 0.000 claims abstract description 28
- 241000196324 Embryophyta Species 0.000 claims abstract description 27
- 230000001488 breeding effect Effects 0.000 claims abstract description 22
- 238000009395 breeding Methods 0.000 claims abstract description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 15
- 239000003337 fertilizer Substances 0.000 claims abstract description 14
- 239000003966 growth inhibitor Substances 0.000 claims abstract description 8
- 238000001035 drying Methods 0.000 claims abstract description 7
- 238000009736 wetting Methods 0.000 claims abstract description 7
- 235000016709 nutrition Nutrition 0.000 claims abstract description 5
- 241000596168 Robbauera albescens Species 0.000 claims abstract description 4
- 230000035764 nutrition Effects 0.000 claims abstract description 4
- 238000007726 management method Methods 0.000 claims description 15
- 238000005286 illumination Methods 0.000 claims description 12
- 241001582888 Lobus Species 0.000 claims description 8
- 230000024241 parasitism Effects 0.000 claims description 7
- 230000001105 regulatory effect Effects 0.000 claims description 4
- UUTKICFRNVKFRG-WDSKDSINSA-N (4R)-3-[oxo-[(2S)-5-oxo-2-pyrrolidinyl]methyl]-4-thiazolidinecarboxylic acid Chemical compound OC(=O)[C@@H]1CSCN1C(=O)[C@H]1NC(=O)CC1 UUTKICFRNVKFRG-WDSKDSINSA-N 0.000 claims description 3
- 206010023126 Jaundice Diseases 0.000 claims description 3
- 239000012141 concentrate Substances 0.000 claims description 3
- 239000000284 extract Substances 0.000 claims description 3
- 239000002689 soil Substances 0.000 claims description 2
- 230000002787 reinforcement Effects 0.000 claims 1
- 238000009825 accumulation Methods 0.000 abstract 1
- 238000012545 processing Methods 0.000 description 29
- 238000004382 potting Methods 0.000 description 12
- 241001305034 Aphidius gifuensis Species 0.000 description 7
- 238000004519 manufacturing process Methods 0.000 description 6
- 238000011835 investigation Methods 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 3
- 238000009826 distribution Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000002015 leaf growth Effects 0.000 description 3
- 238000005057 refrigeration Methods 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000011017 operating method Methods 0.000 description 2
- 230000005855 radiation Effects 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 241001600407 Aphis <genus> Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241000257303 Hymenoptera Species 0.000 description 1
- 206010020880 Hypertrophy Diseases 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000010835 comparative analysis Methods 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 230000009036 growth inhibition Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 230000003014 reinforcing effect Effects 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a kind of efficient numerous bee methods that raising aphid parasite deadlock aphid forms equilibrium degree, the described method comprises the following steps：Step (1) inhibits to float wet cigarette strain apical dominance；The balanced numerous aphid of step (2)：Kind of aphid is connect on the blade guided to and float wet cigarette strain lower part, cigarette aphid is appointed voluntarily to spread breeding, growth inhibitor is smeared after removing axillary bud, continues to inhibit apical dominance；Step (3) is balanced parasitic：When average aphid amount reaches required value in the wet cigarette strain of drift, no hyperparasite bee is accessed at bee and is covered in net, the outer covering sunshade net of net is covered during connecing bee；Step (4) collects stiff aphid.Present invention incorporates cigarette strain bearing capacities to facilitate with floating tobacco seedlings management by force, the advantage that the period is short, water and fertilizer management is carried out by alternation of wetting and drying method simultaneously, cultivate robust plant, the wet cigarette strain of drift that blade children is tender, cigarette aphid likes feeding, it is handled by pinching suckering during follow-up numerous aphid, numerous bee, promote the accumulation of middle and lower part leaf nutrition, makes the balanced breeding on each position blade of cigarette aphid.
Description
Technical field
The invention belongs to aphid parasite deadlock aphid reproduction technique fields more particularly to a kind of raising aphid parasite deadlock aphid to form equilibrium degree
Efficient numerous bee method.
Background technology
Aphidius gifuensis is cigarette aphid and the important insect parasite of other aphids, is widely used on tobacco and other crops and prevents
Eliminate aphis, it is main at this stage using floating tobacco seedlings, plant or the numerous bee of potting cigarette strain mode, discharge stiff aphid seedling or at bee mode
Be difficult to meet the needs of popularization and application, be badly in need of in production can long-acting refrigeration, convenience in transport and release stiff aphid product, efficiently breed
The neat stiff aphid of development is the premise of stiff aphid production.
Application No. is 200810187795.6 Chinese patent applications to disclose a kind of side for breeding aphidius gifuensis using cigarette strain
The cigarette strain of a group of growing to phase is implanted into numerous bee canopy by method, method first, and cigarette strain meets aphid 20- when growing to 9-12 piece effective blades
30, aphid amount it is numerous to 2000-3000 when connect bee, connect 14-15 days stiff aphids after bee and initially form；Or bee is not connect, continued propagation cigarette
Aphid is as kind of an aphid.Single plant bee yield is accumulative up to 8000 in entire numerous bee period, every square metre of 2-4 ten thousand.
Application No. is 201410399950.6 Chinese patent applications to disclose a kind of utilization floating tobacco seedlings breeding aphidius gifuensis
Method, by appropriate leaf-cutting nurturing staff, aphid is connect when tobacco seedlings are grown to 61 heart of leaf first method by 10 every plant or so, when
By 1 when aphid amount reaches 150 heads/plant：50 bee aphid ratios connect bee, connect tight cover net after bee, and about 15-20 days bee yields reach high after connecing bee
The peak phase.Entire numerous every square metre of bee yield of bee period is up to 100,000.
It is found by comparing test to both numerous bee methods, cigarette strain bearing capacity is strong, single plant bee yield is high, breeds stiff aphid
Or aphid parasite is big strong, but water and fertilizer management is inconvenient, unit area bee yield is relatively low, and numerous bee period is long.It is numerous to float tobacco seedlings
Bee method tobacco seedlings bearing capacity is weak, stiff aphid or aphid parasite are more small and weak, but water and fertilizer management is convenient, numerous bee period is short, the numerous bee of unit area
Amount is high.In addition to this, both methods exists simultaneously a stiff aphid and forms irregular, the cigarette strain upper, middle and lower blade deadlock aphid formation time
Skimble-scamble problem, it has not been convenient to collect and dissipate, especially be affected to the collection of aphid parasite deadlock aphid.
Since current those skilled in the art are when breeding aphid parasite, it is provided to meet local vega and other agricultures
Field uses, and by stiff aphid seedling, stiff aphid leaf or at field adaptability is used in the form of bee, conevying efficiency is low, it is largely dead to easily cause aphid parasite
It dies, radiation radius generally rarely exceeds 50 kilometers；Using " personal autotrophy ", " with supporting with putting " mode in production, it is not concerned with aphid cocoon
The harmony and regularity problem that bee deadlock aphid is formed.Therefore it is badly in need of the stiff aphid production of long-acting refrigeration, convenience in transport and release in producing
Product.The processing of aphid parasite deadlock aphid product needs the stiff aphid for largely developing neat and consistent.
Invention content
In view of the deficiencies of the prior art, the present invention provides a kind of efficient numerous bee side of raising aphid parasite deadlock aphid formation equilibrium degree
Method, the present invention is by inhibiting tobacco seedlings apical dominance, control illumination, reinforcing water and fertilizer management, in time the technologies hand such as removing base portion old leaf
Section can improve the harmony of the numerous aphid of cigarette strain Different sites of leaf and numerous aphid efficiency, improve parasitic harmony and parasitic efficiency,
The regularity for improving stiff aphid astogeny, convenient for stiff aphid collection, product processing and product refrigeration.
Technical scheme is as follows：A kind of efficient numerous bee method for improving aphid parasite deadlock aphid and forming equilibrium degree, it is described
Method includes the following steps：
Step (1) inhibits to float wet cigarette strain apical dominance：The consistent stalwartness of establiss on the special numerous bee drift disk in large aperture
Tobacco seedlings are cultivated into cigarette strain using the water and fertilizer management method of alternation of wetting and drying, when cigarette strain enters prosperous long peak period, extract lobus cardiacus and base portion
Jaundice old leaf, smears growth inhibitor at lobus cardiacus and at axil；
The balanced numerous aphid of step (2)：Pure, healthy and strong kind aphid is connect on the blade guided to and float wet cigarette strain lower part, appoints cigarette aphid certainly
Row diffusion breeding, growth inhibitor is smeared after removing the axillary bud newly sprouted, continues to inhibit apical dominance, reinforce water and fertilizer management and and
When remove base portion old leaf, make the balanced numerous aphid of each level tobacco leaf in cigarette strain upper, middle and lower, improve overall numerous aphid amount；
Step (3) is balanced parasitic：When average aphid amount reaches required value in the wet cigarette strain of drift, by being connect at bee for no hyperparasite bee
Enter in cover net, cover net is outer during connecing bee covers sunshade net, avoids direct sunlight, makes uniform illumination in cover net, promotes into bee equilibrium
Aphid on the parasitic each level tobacco leaf in cigarette strain upper, middle and lower avoids first parasitic lower part aphid, rear parasitic middle and upper part aphid phenomenon, makes
Stiff aphid is concentrically formed on whole cigarette, improves overall stiff aphid amount；
Step (4) collects stiff aphid：The blade for winning a large amount of stiff aphids removes cigarette aphid, parasitic aphid, stiff aphid shell with banister brush
Afterwards, stiff aphid is collected, for making stiff aphid product with electronic stiff aphid collection device, or is used for field adaptability.
Further, it is 66cm × 33cm that the strong sprout that conventional seedbed system method is cultivated is transplanted to length × width × height by step (1)
× 8.0cm, 6 holes, aperture 7cm drift disk on cultivate, seedling medium：Nutrition Soil=1：0.8~1.2, using the water of alternation of wetting and drying
Fertile management method is cultivated into cigarette strain, when cigarette strain enters prosperous long peak period, extracts lobus cardiacus and base portion turns to be yellow old leaf, at lobus cardiacus and leaf
Growth inhibitor is smeared at armpit, to promote remaining leaf growth, tobacco seedlings are cultivated into robust plant, blade is light green, particularly in
The well-fed wet cigarette strain of drift of lower blade.
Further, step (2) connects pure, healthy and strong kind aphid on the blade guided to and float wet cigarette strain lower part, and every plant connects aphid
25~30, cigarette aphid is appointed voluntarily to spread breeding, 12~14 days cigarette aphids are largely formed after connecing aphid, with banister brush close in upper blade
The cigarette aphid of collection, which is swept, to be fallen on the less blade of middle and lower part aphid, and the axillary bud newly sprouted is rejected during numerous aphid and smears growth inhibition
Water and fertilizer management is reinforced in agent, and the leaf growth of the cigarette strain of pinching suckering, each level is all vigorous, and numerous aphid amount is high, and is evenly distributed, and carries
High overall numerous aphid amount.
Further, step (3) is when average aphid amount reaches 600~800 heads/plant in the wet cigarette strain of drift, by bee：Aphid ratio is 1：
10~20 connect bee amount, by no hyperparasite bee at bee by suitable bee aphid than in access cover net, with the bilayer of shading rate 75%
Sunshade net covers numerous bee hut, packs up or be unfolded sunshade net according to light conditions, regulating illumination intensity, at during bee parasitism,
It keeps low-light condition, intensity of illumination to be less than 1000Lux in cover net, promotes into each level cigarette in the balanced parasitic cigarette strain upper, middle and lower of bee
Aphid on leaf avoids first parasitic lower part aphid, rear parasitic middle and upper part aphid phenomenon, stiff aphid on whole cigarette is made to be concentrically formed,
Improve overall stiff aphid amount.
Further, step (4) collects stiff aphid when stiff aphid concentrates a large amount of formed：Having won a large amount of stiff aphids, (stiff aphid rate reaches
To 70~80%) blade, after removing cigarette aphid, parasitic aphid, stiff aphid shell with banister brush, or collected with electronic stiff aphid collection device stiff
Aphid, for making stiff aphid product, or be used for field adaptability.
The stiff aphid of breeding is operated in 50~60 square metres that 60 mesh gauzes and sunshade net build, in high 1.8~2.2 meters of cover net
Carry out, according to float wet cigarette strain cultivate, during numerous aphid and numerous bee to the needs of illumination and temperature condition, expansion or collapse sunshade net with
Regulating illumination intensity carries out water and fertilizer management using alternation of wetting and drying method, when aphid amount is more in upper blade or collects stiff aphid blade
When, cigarette aphid and parasitic aphid are swept and fallen on the less blade of aphid with banister brush, avoids wasting.
One of the innovative point of the present invention is that, since cigarette aphid has tender property, cigarette aphid is mostly on cigarette strain top under normal conditions
Hold tender shoots and upper tobacco leaf breeding, the numerous aphid amount of middle and lower part blade less；Kind bee also likes the parasitism under dim light, mostly first under parasitism
The cigarette aphid of portion's blade, the new cigarette aphid at bee parasitic middle and upper part again cause stiff aphid to gradually form from the bottom to top；In addition cigarette strain top
Cigarette aphid it is more, it is also much upper lower few to cause stiff aphid to be distributed in cigarette strain, be typically all after being bred at present due to aphid parasite supply this
Ground uses, and radiation radius is no more than 50 kilometers, using " personal autotrophy ", " with supporting with putting " mode in production, is not concerned with aphid parasite
The harmony and regularity problem that stiff aphid is formed.But the present invention supplies the whole nation or even the world to mass produce stiff aphid product
Various regions, therefore raising aphid parasite deadlock aphid is needed to form harmonious and regularity, convenient for the concentrated collection of stiff aphid, improve the quality of deadlock aphid
And utilization rate.
In order to achieve the above object, the present invention pays close attention to aphid parasite deadlock aphid and forms balanced efficiently numerous bee method, passes through suppression
The apical dominance of tobacco curing strain, it is ensured that the tobacco leaf of cigarette strain lower part is fleshy hypertrophy and nutritional sufficiency, promotes cigarette aphid in different parts tobacco leaf
Upper natural selection equilibrium breeding, improve middle part, lower blade numerous aphid amount, harmony improves, the numerous aphid amount of totality per cigarette strain
It improves therewith.Harmony improves simultaneously, is just convenient for concentrated collection, long-distance transport and the logistics of stiff aphid.
The two of the innovative point of the present invention are that the present invention carries out connecing bee under balanced low light condition；The present invention passes through light
According to adjusting, scattering light in numerous bee cover net is made to be uniformly distributed, promotes into the balanced parasitic cigarette strain top of bee, middle part, lower each position tobacco leaf
On cigarette aphid, Jiang Ya groups concentrate a large amount of formation, avoid first parasitic lower part aphid, rear parasitic middle and upper part aphid within a short period of time
Worm phenomenon makes stiff aphid on whole cigarette be concentrically formed, and improves overall stiff aphid amount.
Compared with prior art, the invention has the advantages that：Present invention incorporates cigarette strain bearing capacity by force with floating
The advantage that tobacco seedlings management is convenient, the period is short, while water and fertilizer management is carried out by alternation of wetting and drying method, cultivate robust plant, blade
Young tender, the hobby feeding of cigarette aphid the wet cigarette strain of drift, is handled by pinching suckering during follow-up numerous aphid, numerous bee, promotes middle and lower part
Leaf nutrition accumulates, and makes the balanced breeding on each position blade of cigarette aphid.By regulating and controlling illumination during connecing bee, posted in avoiding kind from swarming together
Cigarette aphid in raw lower blade, make the wet cigarette strain of drift up and down it is parasitic uniformly, obtained stiff aphid and formed that regularity is high, is evenly distributed and always
The method for measuring the wet stiff aphid of cigarette strain breeding of higher drift.
Specific implementation mode
Technical scheme of the present invention is described in further details with reference to specific embodiment, but the present invention does not limit to
In following technical scheme.
The different numerous numerous aphid effects of aphid method of embodiment 1 compare
The breeding process that kind aphid has been carried out using this method in 2017, with aphidius gifuensis Ashmaed scale propagation technique (application number：
200810187795.6) the numerous aphid of potting cigarette strain in and a kind of method (application number of floating tobacco seedlings breeding aphidius gifuensis：
201410399950.6) in the numerous aphid comparison of floating tobacco seedlings, achieve good effect.Operating procedure and investigation result are as follows：
1, carrier cigarette strain (tobacco seedlings) is cultivated
Handle I (potting cigarette strain method)
By potting cigarette strain law technology requirement, 203 kind of cloud and mist is selected in numerous bee canopy, by being moved after floating seedlings requirement nursery
It plants in flowerpot, and water and fertilizer management is carried out by technology requirement.9 plants every square metre.
Processing II (conventional floating tobacco seedlings method)
By floating tobacco seedlings law technology requirement, in same numerous bee canopy, selection 203 kind of cloud and mist is 66cm with length × width × height
The floating disc of × 33cm × 5.5cm, 162 cave specifications, by floating seedlings requirement seeding and seedling raising.648 plants every square metre.
Processing III (the wet cigarette strain of drift of not pinching)
203 kind of cloud and mist is selected in same numerous bee greenhouse, and few substrate seedling is carried out with 595 holes drift disk.When tobacco seedlings grow to
When the grand cross phase, it is 66cm × 33cm × 8cm, (every square in the nutrient discs in 6 holes (aperture 7.0cm) to be transplanted to length × width × height
24 plants of rice), it is placed in the nursery pond of appropriate compound fertilizer special for flue-cured tobacco aqueous solution, depth of water can suction moisture with Nutrition Soil and be
Preferably；When Nutrition Soil surface has it is a small amount of dry when, spray tobacco foliage fertilizer in time, keep Nutrition Soil moisture sufficient, it is extensive with sharp tobacco seedlings
Demutation is long.After water is dry, tobacco leaf occur slightly withering here shape when, composite fertilizer's aqueous solution is replenished in time, so moves in circles.Tobacco seedlings
To during growing 1 young leaves after transplanting, expansion sunshade net is to reduce illumination；After most tobacco seedlings grow 1 young leaves, sunshade is collapsed
Net enhances illumination, with sharp tobacco seedlings fast-growth.
Handle IV (this method)
203 kind of cloud and mist is selected in same numerous bee greenhouse, and few substrate seedling is carried out with 595 holes drift disk.When tobacco seedlings grow to
When the grand cross phase, it is 66cm × 33cm × 8cm, (every square in the nutrient discs in 6 holes (aperture 7.0cm) to be transplanted to length × width × height
24 plants of rice), it is placed in the nursery pond of appropriate compound fertilizer special for flue-cured tobacco aqueous solution, depth of water can suction moisture with Nutrition Soil and be
Preferably；When Nutrition Soil surface has it is a small amount of dry when, spray tobacco foliage fertilizer in time, keep Nutrition Soil moisture sufficient, it is extensive with sharp tobacco seedlings
Demutation is long.After water is dry, tobacco leaf occur slightly withering here shape when, composite fertilizer's aqueous solution is replenished in time, so moves in circles.Tobacco seedlings
To during growing 1 young leaves after transplanting, expansion sunshade net is to reduce illumination；After most tobacco seedlings grow 1 young leaves, sunshade is collapsed
Net enhances illumination, with sharp tobacco seedlings fast-growth.Cigarette strain enters prosperous long peak period (11~12 true leaves), extracts lobus cardiacus and base in time
1~2, portion jaundice old leaf, and 300 times of flumetralims (the special Suckering agents of tobacco) are smeared at lobus cardiacus and at axil, it is given birth to sharp tobacco leaf
Long and nutrient accumulation.
2, aphid, numerous aphid are connect
Handle I (potting cigarette strain method)
By the numerous bee technology requirement of potting cigarette strain, carry out connecing aphid when cigarette strain grows 11~12 true leaves, under every cigarette strain
On 3, portion leaf, pure kind of aphid aphid 25~30 is connect, cigarette aphid is appointed voluntarily to spread breeding.Reinforce water and fertilizer management, numerous aphid is in the process with 60
Mesh fly net seals.
Processing II (the numerous aphid of conventional floating tobacco seedlings method)
By floating tobacco seedlings law technology requirements, when tobacco seedlings have 5 true leaves, by float leaf of tobacco seedling leaf shearing requirement straight snips,
3 days after leaf-cutting, when tobacco seedlings form the 6th true leaf, pure kind of aphid leaf is laid in tobacco seedlings, blade back downwards, appoints cigarette aphid voluntarily
Diffusion changes to kind of an aphid leaf in new tobacco seedlings when averagely there is about 10 heads/plant of cigarette aphid in tobacco seedlings.Reinforce water and fertilizer management, numerous aphid mistake
It is sealed using 60 mesh fly nets in journey.
Processing III (the wet cigarette strain of drift of not pinching)
It is floated on wet 3 leaves in cigarette strain lower part at every, connects pure kind of aphid aphid 25~30, cigarette aphid is appointed voluntarily to spread breeding.It connects
12~14 days cigarette aphids are largely formed after aphid, it is found that when upper blade aphid amount is more, with banister brush cigarette intensive in upper blade
Aphid, which is swept, to be fallen on the less blade of middle and lower part aphid；The axillary bud newly sprouted is rejected during numerous aphid in time and smears growth inhibitor,
Reinforce water and fertilizer management, is sealed with 60 mesh fly nets during numerous aphid.
Handle IV (the method for the present invention)
It is floated on wet 3 leaves in cigarette strain lower part at every, connects pure kind of aphid aphid 25~30, cigarette aphid is appointed voluntarily to spread breeding.It connects
12~14 days cigarette aphids are largely formed after aphid, it is found that when upper blade aphid amount is more, with banister brush cigarette intensive in upper blade
Aphid, which is swept, to be fallen on the less blade of middle and lower part aphid；The axillary bud newly sprouted is rejected during numerous aphid in time and smears growth inhibitor,
Reinforce water and fertilizer management, is sealed with 60 mesh fly nets during numerous aphid.
3, investigation statistics
When cigarette aphid is largely formed, each numerous aphid situation of processing cigarette strain is investigated by five point samplings, is investigated 1 time within every 3 days, until
Until aphid amount starts to be substantially reduced.It from top to bottom investigates by leaf when investigation, is compared with the data that each processing aphid amount peaks
Compared with.
4, result and analysis
It takes temperature from numerous aphid, the 37th day aphid amount reaches maximum value after I (potting cigarette strain) of processing connects aphid, although single plant aphid amount is high,
Reach 18780 heads/plant, since plantable cigarette strain is few in unit area, the aphid amount in unit area is minimum, only 75120/
m2, far below other processing；The 21st day aphid amount reaches maximum value after processing II (floating tobacco seedlings) connects aphid, the aphid amount in unit area
Highest, but aphid individual is small, and it is small that aphid parasite does not like the stiff aphid formed after parasitism or parasitism；(the wet cigarette of drift of not pinching of processing III
Strain) and handle IV (this method) connect aphid after the 31st day aphid amount reach maximum value, although single plant aphid amount is low compared with potting cigarette strain, due to list
Plantable cigarette strain is more in plane product, and the aphid amount in unit area is far above potting cigarette strain；Handle the single plant aphid of IV (this method)
It measures and unit area aphid amount is far above processing III (the wet cigarette strain of drift of not pinching) again, and the difference of aphid individual and potting cigarette strain is not
Greatly, it is parasitic to be suitable for aphid parasite.It is shown in Table 1.
Table 1 respectively handles maximum numerous aphid amount and its is distributed on blade
From aphid amount in terms of the distribution on blade, I (potting cigarette strain) 3, top tree louse amount of processing accounts for the 53.62% of plant aphid amount,
3, middle part tree louse amount accounts for the 41.11% of plant aphid amount, i.e. middle and upper part aphid amount accounts for the 94.73% of plant aphid amount, and the tree louse amount of lower part 3 accounts for
The 5.27% of strain aphid amount；Processing III (the wet cigarette strain of drift of not pinching) 3, top tree louse amount accounts for the 59.49% of plant aphid amount, 3, middle part leaf
Aphid amount accounts for the 33.44% of plant aphid amount, i.e. middle and upper part aphid amount accounts for the 92.93% of plant aphid amount, and the tree louse amount of lower part 3 accounts for plant aphid amount
7.07%；IV 3, (this method) top tree louse amount of processing accounts for the 39.60% of plant aphid amount, and 3, middle part tree louse amount accounts for plant aphid amount
33.63%, i.e. middle and upper part aphid amount accounts for the 73.23% of plant aphid amount, and the tree louse amount of lower part 3 accounts for the 26.77% of plant aphid amount.It is shown in Table 2.
I.e. IV (this method) numerous aphid of processing optimizes in quality and quantity.
The numerous aphid aphid amount of 2 cigarette strain of table is distributed on blade
The different numerous bee method deadlock aphid breeding effects of embodiment 2 compare
Embodiment 1 is after obtaining good numerous aphid effect, the breeding that aphid parasite deadlock aphid has been carried out using this method in 2017
Work is further examined the stiff aphid breeding effect of different numerous bee methods, with existing invention aphidius gifuensis Ashmaed scale propagation
Technique (application number：200810187795.6) potting cigarette strain method, a kind of method (application of floating tobacco seedlings breeding aphidius gifuensis
Number：201410399950.6) floating tobacco seedlings method, the wet numerous bee deadlock aphid breeding effect of cigarette strain of drift of not pinching have done comparative analysis, grasp
It is as follows with result to make step：
Numerous aphid operating procedure is same as Example 1.
1, bee, numerous bee are connect
To avoid bee form not of the same race and connecing influence of the bee amount to parasitic efficiency, the present embodiment uniformly connects stiff aphid seedling, connects
Bee amount uniformly presses bee：Aphid ratio is 1：10 calculate；Each processing carries out in same greenhouse.
Handle I (potting cigarette strain method)
Requirement of the bee opportunity to aphid amount is connect by the numerous bee of potting cigarette strain, connects after aphid the 28th day, when aphid amount reaches 2000~3000
Head/plant when connect bee, at the top of numerous bee cover net cover shading rate 75% sunshade net.
Processing II (conventional floating tobacco seedlings method)
Connect requirement of the bee opportunity to aphid amount by the floating numerous bee of tobacco seedlings, connect after aphid the 13rd day, about 150/disk of aphid amount when connect bee,
The sunshade net of shading rate 75% is covered at the top of numerous bee cover net.
Processing III (the wet cigarette strain of drift of not pinching)
Connect after aphid the 17th day, aphid amount reach 600~800 heads/plant and connect bee, shading rate 75% is covered at the top of numerous bee cover net
Sunshade net.
Handle IV (this method)
Connect after aphid the 18th day, aphid amount reach 600~800 heads/plant and connect bee, at the top of numerous bee cover net and surrounding covers shading rate
Sunshade net, regulating illumination intensity, especially during connecing bee are packed up according to light conditions or covered to 75% double-layered sunshade net
First 4 days, cover net is interior to keep 1000Lux low-light conditions below, enable into each position cigarette in the balanced parasitic cigarette strain upper, middle and lower of bee
Cigarette aphid on leaf.
2, investigation statistics
When stiff aphid is largely formed, each numerous bee situation of processing cigarette strain is investigated by five point samplings, investigation 1 time daily, until
Until stiff aphid amount is substantially reduced.When investigation respectively statistically 3, portion leaf, 3, middle part leaf and the leaf of lower part 3 parasitic aphid quantity,
Effectively deadlock aphid quantity and stiff aphid quantity of sprouting wings are compared with the data that each processing aphid amount peaks.
3, result and analysis
In terms of bee yield, the 26th day bee yield reaches maximum value after I (potting cigarette strain) of processing connects bee, due in unit area
Numerous aphid amount it is low, cause numerous bee bee amount also low, only 55272/m2, far below other processing；Processing II (floating tobacco seedlings) connects
The 18th day bee amount reaches maximum value after bee, and the bee amount highest in unit area reaches 119808/m2, but the stiff aphid formed is small,
It is second-rate；The 25th day bee amount reaches maximum value after processing III (do not pinch drift wet cigarette strain) connects bee, the bee amount in unit area compared with
Height reaches 78672/m2；Processing IV (this method) connect bee after the 20th day bee amount reach maximum value, the bee amount in unit area with
Numerous bee of processing II (floating tobacco seedlings) is suitable, reaches 118584/m2.It is shown in Table 3.
The overall bee yield of the different numerous bee methods of table 3
In terms of the harmony of numerous bee, the bee yield of I 3 leaves in (potting cigarette strain) top of processing accounts for the 64.09% of whole strain, distribution
It is least balanced；The bee yield of processing III (the wet cigarette strain of drift of not pinching) 3 leaves in top accounts for the 52.56% of whole strain, and distribution is also unbalanced；
Processing IV 3, (this method) top leaf, 3, middle part leaf, 3 leaves in lower part bee yield account for respectively whole strain 38.78%,
37.06%, 24.17%, distributing equilibrium.It is shown in Table 4.
The different numerous numerous bees of bee method of table 4 are harmonious
Final purpose of the present invention is not only the harmony of numerous bee, it is often more important that it is numerous go out more effectively stiff aphid --- non-plumages
Change stiff aphid.Effective stiff aphid of IV (this method) of processing reaches 69600/m2, it is far above other numerous bee methods, is I (potting of processing
Cigarette strain) 3.54 times, 1.95 times of processing II (floating tobacco seedlings), 1.83 times of processing III (the wet cigarette strain of drift of not pinching).Stiff aphid is big
It is small suitable with potting cigarette strain.It is shown in Table 5.
Effective stiff aphid amount (head/m of the different numerous bee method unit areas of table 52)
It can be seen that based on the above results, the present invention uses many kinds of measures, improves the harmony of numerous aphid, numerous bee, not only improves
The effectively stiff aphid amount of unit, also improves the regularity that stiff aphid is formed, this to stiff aphid efficiently use and production is particularly heavy
It wants.
Claims (6)
1. a kind of efficient numerous bee method for improving aphid parasite deadlock aphid and forming equilibrium degree, which is characterized in that the method includes following
Step：
Step (1) inhibits to float wet cigarette strain apical dominance：The consistent healthy and strong tobacco seedlings of establiss on the special numerous bee drift disk in large aperture,
It is cultivated into cigarette strain using the water and fertilizer management method of alternation of wetting and drying, when cigarette strain enters prosperous long peak period, extracts lobus cardiacus and base portion jaundice
Old leaf smears growth inhibitor at lobus cardiacus and at axil；
The balanced numerous aphid of step (2)：Pure, healthy and strong kind aphid is connect on the blade guided to and float wet cigarette strain lower part, cigarette aphid is appointed voluntarily to expand
Breeding is dissipated, growth inhibitor is smeared after removing the axillary bud newly sprouted, continues to inhibit apical dominance, reinforcement water and fertilizer management simultaneously clear in time
Except base portion old leaf, makes the balanced numerous aphid of each level tobacco leaf in cigarette strain upper, middle and lower, improve overall numerous aphid amount；
Step (3) is balanced parasitic：When average aphid amount reaches required value in the wet cigarette strain of drift, no hyperparasite bee is accessed at bee and is covered
Cover net is outer in net, during connecing bee covers sunshade net, avoids direct sunlight, makes uniform illumination in cover net, it is balanced parasitic to promote into bee
Aphid on each level tobacco leaf in cigarette strain upper, middle and lower avoids first parasitic lower part aphid, rear parasitic middle and upper part aphid phenomenon, makes whole
Stiff aphid is concentrically formed on cigarette, improves overall stiff aphid amount；
Step (4) collects stiff aphid：The blade for winning a large amount of stiff aphids, after removing cigarette aphid, parasitic aphid, stiff aphid shell with banister brush, with
Stiff aphid collection device collects stiff aphid, for making stiff aphid product, or is used for field adaptability.
2. improving efficient numerous bee method that aphid parasite deadlock aphid forms equilibrium degree as described in claim 1, which is characterized in that step
(1) strong sprout that conventional seedbed system method is cultivated is transplanted to seedling medium：Nutrition Soil=1：It cultivates in 0.8~1.2, is handed over using dry and wet
The water and fertilizer management method replaced is cultivated into cigarette strain, when cigarette strain enters prosperous long peak period, lobus cardiacus and base portion jaundice old leaf is extractd, in lobus cardiacus
Growth inhibitor is smeared at place and axil, to promote lower blade to grow.
3. improving efficient numerous bee method that aphid parasite deadlock aphid forms equilibrium degree as described in claim 1, which is characterized in that step
(2) pure, healthy and strong kind aphid is connect on the blade guided to and float wet cigarette strain lower part, every plant connects aphid 25~30, and cigarette aphid is appointed voluntarily to expand
Breeding is dissipated, 12~14 days cigarette aphids are largely formed after connecing aphid, and cigarette aphid intensive in upper blade is swept with banister brush and falls on middle and lower part
On the less blade of aphid, the axillary bud newly sprouted is rejected during numerous aphid and smears growth inhibitor, reinforce water and fertilizer management.
4. improving efficient numerous bee method that aphid parasite deadlock aphid forms equilibrium degree as described in claim 1, which is characterized in that step
(3) when average aphid amount reaches 600~800 heads/plant in the wet cigarette strain of drift, by bee：Aphid ratio is 1：10~20 connect bee amount, will be without weight
Parasitic wasp is accessed at bee in cover net, covers numerous bee hut with the double-layered sunshade net of shading rate 75%, is packed up according to light conditions
Or expansion sunshade net, regulating illumination intensity keep low-light condition, illumination to be less than at during bee parasitism in cover net
1000Lux。
5. improving efficient numerous bee method that aphid parasite deadlock aphid forms equilibrium degree as described in claim 1, which is characterized in that step
(4) when stiff aphid concentrates a large amount of formed, stiff aphid is collected：The blade for winning a large amount of stiff aphids, cigarette aphid, parasitism are removed with banister brush
After aphid, stiff aphid shell, stiff aphid is collected, for making stiff aphid product with stiff aphid collection device, or be used for field adaptability.
6. improving efficient numerous bee method that aphid parasite deadlock aphid forms equilibrium degree as described in claim 1, which is characterized in that breeding
Stiff aphid is operated in 50~60 square metres, the interior progress of high 1.8~2.2 meters of cover net that 60 mesh gauzes and sunshade net are built, according to drift
To the needs of illumination and temperature condition during wet cigarette strain cultivation, numerous aphid and numerous bee, expansion or gathering sunshade net are strong to adjust illumination
Degree carries out water and fertilizer management using alternation of wetting and drying method, when aphid amount is more in upper blade or collects stiff aphid blade, uses banister brush
Cigarette aphid and parasitic aphid are swept and fallen on the less blade of aphid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810088840.6A CN108419758B (en) | 2018-01-30 | 2018-01-30 | Efficient bee breeding method for improving stiff aphid formation balance degree of aphidiidae |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810088840.6A CN108419758B (en) | 2018-01-30 | 2018-01-30 | Efficient bee breeding method for improving stiff aphid formation balance degree of aphidiidae |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108419758A true CN108419758A (en) | 2018-08-21 |
| CN108419758B CN108419758B (en) | 2021-09-24 |
Family
ID=63156153
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810088840.6A Active CN108419758B (en) | 2018-01-30 | 2018-01-30 | Efficient bee breeding method for improving stiff aphid formation balance degree of aphidiidae |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108419758B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110771577A (en) * | 2019-11-18 | 2020-02-11 | 西南林业大学 | High-density rapid breeding method for stiff aphids of aphidius gifuensis ashmeads |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101455190A (en) * | 2008-12-30 | 2009-06-17 | 云南省烟草公司玉溪市公司 | Aphidius gifuensis Ashmaed scale propagation technique |
| CN101664017A (en) * | 2009-09-30 | 2010-03-10 | 云南省烟草农业科学研究院 | Method for propagating sulzer braconid flies |
| CN102388842A (en) * | 2011-08-04 | 2012-03-28 | 云南省烟草公司保山市公司 | Method for breeding and feeding Aphidius gifuensis Ashmesd in floating seedling raising shed |
| CN202738649U (en) * | 2012-09-12 | 2013-02-20 | 云南省烟草公司红河州公司 | Simple insect feeding shed |
| CN104126551A (en) * | 2014-08-15 | 2014-11-05 | 云南省烟草公司红河州公司 | Method for breeding aphidius gifuensis ashmead through floating tobacco seedlings |
| RU2535363C2 (en) * | 2013-03-22 | 2014-12-10 | Федеральное государственное бюджетное учреждение науки Полярно-альпийский ботанический сад-институт Кольского научного центра Российской академии наук (ПАБСИ КНЦ РАН) | METHOD OF JOINT GROWING OF Aphidoletes aphidimyza AND Aphidius colemani |
| CN107372372A (en) * | 2017-09-08 | 2017-11-24 | 云南省烟草公司红河州公司 | A kind of method for regenerating cigarette strain breeding cigarette aphid and aphidius gifuensis |
| CN107455341A (en) * | 2017-08-01 | 2017-12-12 | 中国烟草总公司福建省公司 | A kind of aphidius gifuensis mating system of regulation and control host crop growth |
-
2018
- 2018-01-30 CN CN201810088840.6A patent/CN108419758B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101455190A (en) * | 2008-12-30 | 2009-06-17 | 云南省烟草公司玉溪市公司 | Aphidius gifuensis Ashmaed scale propagation technique |
| CN101664017A (en) * | 2009-09-30 | 2010-03-10 | 云南省烟草农业科学研究院 | Method for propagating sulzer braconid flies |
| CN102388842A (en) * | 2011-08-04 | 2012-03-28 | 云南省烟草公司保山市公司 | Method for breeding and feeding Aphidius gifuensis Ashmesd in floating seedling raising shed |
| CN202738649U (en) * | 2012-09-12 | 2013-02-20 | 云南省烟草公司红河州公司 | Simple insect feeding shed |
| RU2535363C2 (en) * | 2013-03-22 | 2014-12-10 | Федеральное государственное бюджетное учреждение науки Полярно-альпийский ботанический сад-институт Кольского научного центра Российской академии наук (ПАБСИ КНЦ РАН) | METHOD OF JOINT GROWING OF Aphidoletes aphidimyza AND Aphidius colemani |
| CN104126551A (en) * | 2014-08-15 | 2014-11-05 | 云南省烟草公司红河州公司 | Method for breeding aphidius gifuensis ashmead through floating tobacco seedlings |
| CN107455341A (en) * | 2017-08-01 | 2017-12-12 | 中国烟草总公司福建省公司 | A kind of aphidius gifuensis mating system of regulation and control host crop growth |
| CN107372372A (en) * | 2017-09-08 | 2017-11-24 | 云南省烟草公司红河州公司 | A kind of method for regenerating cigarette strain breeding cigarette aphid and aphidius gifuensis |
Non-Patent Citations (4)
| Title |
|---|
| 杨成云: "烤烟少基质漂浮育苗技术", 《农村实用技术》 * |
| 植保专业生物防治组: "关于用松毛虫赤眼蜂防治玉米螟的试验初报", 《铁岭农学院学报》 * |
| 湖北省烟草科研所: "《湖北省烟草生产技术培训教程》", 31 October 2013, 湖北科学技术出版社 * |
| 郑先福: "《植物生长调节剂应用技术》", 30 June 2013, 中国农业大学出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110771577A (en) * | 2019-11-18 | 2020-02-11 | 西南林业大学 | High-density rapid breeding method for stiff aphids of aphidius gifuensis ashmeads |
| CN110771577B (en) * | 2019-11-18 | 2021-09-24 | 西南林业大学 | High-density rapid breeding method for stiff aphids of aphidius gifuensis ashmeads |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108419758B (en) | 2021-09-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106258766B (en) | The facility cultivation method of 1 year two inferior fruit of knot of large cherry | |
| CN102057851B (en) | New technique for culturing apocarya by seeding and grafting in current year | |
| CN102204489B (en) | Method for culturing teak seedlings by spike culture and rapid propagation in container | |
| CN105210768B (en) | The cultural method of one planting fruit-trees garden interplanting medicine vegetable | |
| CN103430805A (en) | Shaping cultivation method of peach tree shape with two boughs and one center truck | |
| CN103181286B (en) | Method for overcoming topophysis in vegetative propagation of gingkoes | |
| CN105104049B (en) | A kind of naturalization mating system of money loose seedling | |
| CN105165512A (en) | Crown inclination controlling four-season cutting rapid propagation method for alnus formosana | |
| CN104126476B (en) | Fructus actinidiae chinensis bilayer high yield spontaneous pollination implantation methods | |
| CN104756732A (en) | Accurate high-yield high-quality cultivation method for brasenia schreberi | |
| CN102893786B (en) | Paddy-upland rotation culture method for strawberries and water chestnuts | |
| CN109429853A (en) | A kind of Propagation of Actinidia Arguta By Green Cutting method | |
| CN114303693B (en) | Reshaping method for persimmon 'N + 1' tree form | |
| CN108575474A (en) | It is a kind of it is red cone cutting orchard construction and cottage method | |
| CN105210775A (en) | A kind of Changbai Mountain wild actinidia arguta artificial cultivation method | |
| CN109729854A (en) | A kind of silk ball Jia Opulus (V. macrocephalum) cuttage breeding method | |
| CN108739096A (en) | A kind of family implantation methods of radix tetrastigme | |
| CN105340675B (en) | A kind of vinegrowing fruiting management method | |
| CN107114194A (en) | A kind of method of leaf matrimony vine facility whole year production | |
| CN107624402A (en) | A kind of breeding method of Variety of Apple | |
| CN107258293A (en) | A kind of rich root method for culturing seedlings of toon rapid cuttage | |
| CN108419758A (en) | A kind of efficient numerous bee method for improving aphid parasite deadlock aphid and forming equilibrium degree | |
| CN106818386A (en) | A kind of Chinese chestnut hybrid seedling breeding method | |
| CN103262720A (en) | Cedar artificial rapid propagation and transplanting method | |
| CN106416966A (en) | Cutting propagation method of begonia leaf |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address |
Address after: 653100 No. 102 Fenghuang Road, Hongta District, Yunnan, Yuxi Patentee after: YUXI TABACOO COMPANY OF YUNNAN PROVINCE Patentee after: SOUTHWEST FORESTRY University Patentee after: KUNMING RUNCAI AGRICULTURAL TECHNOLOGY DEVELOPMENT CO.,LTD. Address before: No. 300, bailongsi, Panlong District, Kunming City, Yunnan Province Patentee before: SOUTHWEST FORESTRY University Patentee before: YUXI TABACOO COMPANY OF YUNNAN PROVINCE Patentee before: KUNMING RUNCAI AGRICULTURAL TECHNOLOGY DEVELOPMENT CO.,LTD. |
|
| CP03 | Change of name, title or address |