CN103181286B - Method for overcoming topophysis in vegetative propagation of gingkoes - Google Patents

Method for overcoming topophysis in vegetative propagation of gingkoes Download PDF

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CN103181286B
CN103181286B CN201310078685.7A CN201310078685A CN103181286B CN 103181286 B CN103181286 B CN 103181286B CN 201310078685 A CN201310078685 A CN 201310078685A CN 103181286 B CN103181286 B CN 103181286B
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cuttage
cuttings
cutting
ginkgo
stump
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CN103181286A (en
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邢世岩
王聪聪
任娟霞
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Shandong Agricultural University
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Abstract

The invention discloses a method for overcoming topophysis in vegetative propagation of gingkoes, which includes the following steps: taking a healthy and full-sprouted branch being without diseases and insect pests and growing at the middle upper part of a crown on the sunny side as a cutting slip; and adopting the cuttage and stumping method to solve the problem of topophysis during the vegetative propagation of gingkoes. Multiple branches generated after stumping can be directly adopted for cuttage or propagation by grafting, and the main branch can grow vertically. According to the method, the cost is low, the seedling forming speed is high, the rate of survival is high; and the problem of quick breeding of good seed sources, pedigree, clone and excellent single plant of gingkoes can be solved.

Description

Overcome the method for ginkgo vegetative propagation position effect
Technical field
The invention belongs to the vegetative propagation field in woody plant cultivating seedlings, particularly a kind of a kind of technical matters about overcoming ginkgo vegetative propagation position effect.
Background technology
Ginkgo is to carry over after quaternary glacier motion, and the most ancient living fossil world-famous and Relict Plant in existing spermatophyte, is China's special product, is national second class protection seeds.Ginkgo whole body is " treasured ", integrates edible, medicinal, economical, ecological, cultureal value, is most typical peculiar multipurpose seeds.Ginkgo origin is not still cultivated by China, and the ancient center of introduction and acclimatization is also gingko resource and kind diversity is the abundantest and the country of output, export volume maximum in the world.At present by more than 50 country's cultivation in the world.Ginkgo for 1000, reaches 600 year as the history of economic forest cultivation at Chinese cultivation history.
Ginkgo breeding comprises seminal propagation, propagation by grafiting, is propagated from cuttings, organizes and cultivate and burr breeding 5 large classes.At present, grafting and to be propagated from cuttings be vegetative 2 main paties of ginkgo.Propagation by grafiting is the ginkgo kinds such as China, Japan, Korea S one of the major measure of producing of growing directly from seeds.Ginkgo grafting is at least in the existing 400 years history of China.The sixties in 20th century, the U.S. just carried out breeding and the preservation of staminiferous plant improved seeds by this technology.As far back as nineteen forty-seven, the Vermeulen of New Jersey just starts to carry out the research that characteristic of ginkgo male plants is propagated from cuttings, but until just successful by Teuseher reported first to nineteen fifty-one.1950's, the ginkgo technology of being propagated from cuttings caused that domestic many people pay attention to, and this technology of the initial stage nineties is applied on producing.Therefore, grafting and be propagated from cuttings for ginkgo breeding fast breeding, do sth. in advance solid significant.
But, outstanding theory and practice problem of ginkgo grafting and cuttage seeding is the oblique habit of growth of tool, the determining positions on tree body that is merismatic fuselage state by it, and in the process of nourishing and generating, keep stable a kind of biological phenomena, be called position effect (Topophysis).Though position effect have promote flower bud differentiation, make to begin the fruit phase in advance, the oblique and arc of branch is grown to Ginkgo bonsai and makes and established the advantages such as good basis, it has great drawback: (1) has reduced the proliferative speed of breeding elite stand; (2) from material and greening angle, position effect often make breeding the bending of breeding trunk, layering poor, can not reproduce the good growth traits of elite stand; (3) from fruit growing, oblique growth is not easy to the tree-like cultivation of getting bumper crops, and is unfavorable for dense planting.Under this background, a kind of method that overcomes ginkgo vegetative propagation position effect has been proposed, the method utilizes the base portion of cuttage seeding to have a large amount of kryptoblast, kryptoblast easily produces multiple dry, growth, has the advantages that to return young rejuvenation vertically upward, utilizes multiple dry branch grafting or cuttage, trunk can be grown vertically upward, can eliminate position effect.
Summary of the invention
The object of the invention is, there is the phenomenon of oblique growth for cuttage seeding in the vegetative process of ginkgo, for controlling or eliminate the position effect of cuttage, propose a set of complete novel ginkgo vegetative propagation technique technique, reproduce the good growth traits of elite stand, the proliferative speed that promotes breeding elite stand, the tree-like object of cultivation high yield to realize.
In order to achieve the above object, the present invention has adopted following technical scheme:
(1) adopting bar elite stand selects: for the improved seeds that bar elite stand must be the large tree of result of adopting of cottage propagation, fruit is large, high-quality, high yield, or tree-like grace, form satisfactorily material perfectly straight, fast growth use kind select tree;
(2) cuttings is selected and is processed: select robust growth, bud full, without damage by disease and insect cutting, adopt bar in tree crown sunny side middle and upper part, cuttings upper cut is flat nick, lower cut is cut into single horse ear shape with sharp blade or budding knife, spray cutting retains 2-4 sheet leaf, the long 8-10cm of cuttings, base footpath 0.3-0.8cm; The long 10-30cm of hardwood cuttings cuttings, thick 0.4-0.8cm, the long 1.5-2.0cm of bevel;
(3) the epicormic branch cuttage time can carry out in mid-May, and late June transplants; About March 15 cuttage time or Autumn Cutting, spring transplant, cuttage degree of depth 10-15cm; Taper hole straight cutting, after inserting, spray is once permeable;
(4) after cuttage, manage: epicormic branch cuttage requires to control bed surface temperature 30 DEG C of left and right, keep ground moistening, relative air humidity 85% left and right, the cooling of will sheltering from heat or light in cuttage 5-8 month, prevents that matrix from becoming dry, keep relative air humidity more than 80%, daytime temperature is with 21 DEG C-25 DEG C, and be advisable night with 15 DEG C, and the suitableeest soil temperature is 15-20 DEG C, facility cool canopy light transmittance is advisable with 30%-50%, notes the extermination of disease and insect pest;
(5) Ginkgo early growth type, epicormic branch cuttage is then without high growth, and therefore cutting time is to be early advisable, in case there is freeze injury winter, height of seedling 15.2cm then, leading thread 0.4cm; The hard branch of ginkgo gets final product pumping shoot in seedbed, and young sprout germination rate can reach 80% left and right, and after nursery stock transplanting, because seedling-slowing stage is long, the amount of taking root is little then;
(6) stump rejuvenation, stump instrument is shrub and hedge trimmer, and the stump time can be transplanted when latter 1 year, base portion have new life dry again and carry out in cuttage seeding, and while noting stump, crops for rotation want level and smooth, without splitting a batch breach, stubble height 3-5cm; Common 1 strain cuttage seeding can be germinated the multiple dry of 3-5 growth vertically upward after stump, with age growth obviously increase of dry number again, can set up improved seeds cutting orchard by this approach, utilize scion or the cuttings breeding that this cutting orchard obtains can eliminate position effect.
The invention has the beneficial effects as follows:
(1) position effect has been constrained tree body and has been nourished and grown, and often makes the bending of breeding trunk, the layering of breeding poor, " short dry low hat "; This technology makes the good growth traits of elite stand be reproduced.
(2) position effect makes the oblique growth of trunk, is unfavorable for get bumper crops tree-like cultivation and dense planting, makes this phenomenon be overcome and regulate and control by this technology.
(3) effectively promoted the proliferative speed of breeding elite stand.
Utilize said method to utilize the raw multiple dry vegetative propagation of carrying out of base, to reverse the position effect of breeding, reach rejuvenation and delay the object of clone aging, facility is easy, with low cost, for enriching ginkgo seedling-cultivating method, particularly significant for setting up of ginkgo fine provenance, family, clonal reproduction and ginkgo breeding picking leaves garden.
Brief description of the drawings
Fig. 1 cutting processing;
Fig. 2 cuttage root-taking;
Fig. 3 cutting position effect;
The rejuvenation of Fig. 4 stump
Note: 3-1 is band top cutting, and 3-2 is the cutting of stem section; 4-1 is stump, and 4-2 is for multiple dry.
Embodiment
Embodiment 1:
This is studied in 1993-1995 and carries out in ginkgo stock breeding garden, Tancheng, belongs to the little shed of the plastics seedbed in protection ground cuttage.Select tree crown sunny side middle and upper part robust growth, bud full, make cuttings without the branch of damage by disease and insect.Adopt ABT root-inducing powder to process and automatic intermittent spraying cultivation cuttage seeding.Nursery stock is managed same conventional seedbed system.Seedling to minimum position, and stimulates that to produce a large amount of vertical tender tips of regeneration dry again through stump repeatedly; After transplanting, to the dry stump of mother, do to impel normal bud or indefinite bud to germinate again the 2nd year spring, and stump, after 2 years, reverses to the 3rd year position effect continuously.Ginkgo cuttage stump rejuvenation method implementation step is as follows:
1. cuttage preparation
The instrument of preparing cuttage has: shrub and hedge trimmer, single-edge blade, budding knife, Hydra plastic sack, bucket.When cuttage, scion is placed in the plastic sack of moisturizing, with inserting with getting.
2. the selection of cuttings and processing
Spray cutting select robust growth, bud full, without damage by disease and insect, adopt bar in tree crown sunny side middle and upper part, cuttings upper cut is flat nick, above bud, 1cm place is blocked.1cm place below bud, lower cut position is blocked.Lower cut is cut into single horse ear shape with sharp blade or budding knife.Otch will, in the side of bud, retain lateral bud as far as possible.The part of pruning be cuttings footpath thick 1/3, form layer and xylem as long as expose, and it is thicker that reserve part is tried one's best, and is beneficial to take root.Retain 2-4 sheet leaf.The long 8-10cm of cuttings, base footpath 0.3-0.8cm, the long 10-30cm of hardwood cuttings, thick 0.4-0.8cm.
3. cutting time
In ginkgo protection ground, cuttage can be divided into four periods according to the lignified degree of cutting, and spray is from late May to early August cuttage.About March 15 cuttage time or Autumn Cutting, spring transplant.From rooting efficiency, except July is slightly low, rooting rate all can reach more than 95% At All Other Times.
4. cottage method
For preventing that lower cut skin zone from tearing, can adopt taper hole straight cutting or the trench digging method by wall cuttage.After inserting, by the real matrix of hand, prevent that cuttings is unsettled, guarantee that cuttings and matrix connects airtight, and spray once permeable at once.Following maintenance, has good growing environment in slotting machine, ventilation and penetrating light, the overlapped and extruding of the blade of rigorous different cuttings when epicormic branch cuttage.The lateral bud of cuttings topmost is to the south, and blade is all on a line.Under the good condition of spraying facility, shallow the inserting of trying one's best to guarantee that lower cut has good aeration status, is convenient to lifting simultaneously, and spray inserts 5-8cm, cuttage degree of depth 10-15cm.Cutting density 5 × 10cm is advisable.
5. management after inserting
Epicormic branch cuttage requires to control bed surface temperature 30 DEG C of left and right, keep ground moistening, relative air humidity 85% left and right, the cooling of will sheltering from heat or light in cuttage 5-8 month, prevents that matrix from becoming dry, keep relative air humidity more than 80%, daytime temperature is with 21 DEG C-25 DEG C, and be advisable night with 15 DEG C, and the suitableeest soil temperature is 15-20 DEG C, facility cool canopy light transmittance is advisable with 30%-50%, notes the extermination of disease and insect pest.
6. acclimatization and transplants
When rooting rate reach 90%, on average take root several more than 4.After cuttage 45 days, just can acclimatization and transplants.Hardening technology is the practical another key measure of protection ground cuttage.The principle of hardening: strengthen gradually the photosynthesis of the seedling of taking root, the effect of performance protective tissue, strengthens the adaptability of environment to external world.7-15 days hardening time was advisable.During hardening the control of envirment factor with take root during contrary, mainly contain:
(1) prevent under the prerequisite of matrix exsiccation, reduce gradually water spray number of times and injection flow rate, nursery stock is carried out to drought resisting exercise;
(2) increase intensity of illumination.Take off gradually the reed curtain of the side of covering, increase canopy iuuminting rate, until all remove;
(3) increase ventilation number and intensity, reduce humidity in canopy.
When lifting, carefully excavate by strain with scoop, prevent root system damage, and retain rhizosphere matrix around, preferably balled transplanting as far as possible.Ginkgo Cuttings root system, compared with thick and crisp, is easily hindered root when cultivation, therefore should adopt " trench digging is by wall planting method ", that is: first on whole good bed surface, open the shallow ridges of dark 5-10cm, a wide 10cm, and the inwall of ditch is vertical.By the leveling of shallow ridges bottom of trench, then plant by the seeding row spacing of 10 × 30cm.The time of planting is robbed cultivation before being preferably in rain, or successive cloudy days and planting at dusk.After planting, pours water once at once, preferably top dressing every day once, to reduce seedling-slowing stage.General cultivation just can survive for latter 15 days, the same conventional seedbed system of later management.
7. stump rejuvenation
It is one of main feature of this invention that ginkgo cutting is carried out to stump rejuvenation, and stump instrument is shrub and hedge trimmer, and specific requirement is:
(1) selection of best stump time: the stump time can be transplanted when latter 1 year, base portion have new life dry again and carry out in cuttage seeding.Generally in the fall resin stop flow after or spring resin start flow progresses row stump, be preferably annual November 20 to March in next year.
(2) stubble height: toothing is unsuitable too high, and crops for rotation are too high not only to be caused the wasting of resources but also is unfavorable for sprout tillers.General toothing 3-5cm is the most suitable.When stump, crops for rotation want level and smooth, without splitting a batch breach.
(3) stump intensity: when stump, will entirely put down for each strain cutting.
Common 1 strain cuttage seeding can be germinated the multiple dry of 3-5 growth vertically upward after stump, with age growth obviously increase of dry number again, can set up improved seeds cutting orchard by this approach, utilize scion or the cuttings breeding that this cutting orchard obtains can eliminate position effect.
The present invention is not limited to above embodiment, and as long as used the key technologies such as stump rejuvenation in ginkgo cuttage, the measure such as no matter whether coordinate to shade, within all will falling into protection scope of the present invention.

Claims (2)

1. overcome a method for ginkgo vegetative propagation position effect, the method comprises the step of following order:
(1) adopting bar elite stand selects: for the improved seeds that bar elite stand must be the large tree of result of adopting of cottage propagation, fruit is large, high-quality, high yield, or tree-like grace, form satisfactorily material perfectly straight, fast growth use kind select tree;
(2) cuttings is selected and is processed, select robust growth, bud full, without damage by disease and insect cutting, adopt bar in tree crown sunny side middle and upper part, cuttings upper cut is flat nick, lower cut is cut into single horse ear shape with sharp blade or budding knife, spray cutting retains 2-4 sheet leaf, the long 8-10cm of cuttings, base footpath 0.3-0.8cm; The long 10-30cm of hardwood cuttings cuttings, thick 0.4-0.8cm, the long 1.5-2.0cm of bevel;
(3) the epicormic branch cuttage time carried out in mid-May, and late June transplants; About March 15 cuttage time or Autumn Cutting, spring transplant, cuttage degree of depth 10-15cm; Taper hole straight cutting, after inserting, spray is once permeable;
(4) after cuttage, manage epicormic branch cuttage requirement control bed surface temperature 30 DEG C of left and right, keep ground moistening, relative air humidity 85% left and right, the cooling of will sheltering from heat or light in cuttage 5-8 month, prevents that matrix from becoming dry, keep relative air humidity more than 80%, daytime temperature is with 21 DEG C-25 DEG C, and be advisable night with 15 DEG C, and the suitableeest soil temperature is 15-20 DEG C, facility cool canopy light transmittance is advisable with 30%-50%, notes the extermination of disease and insect pest;
(5) ginkgo seedling belongs to early growth type, and epicormic branch cuttage is then without high growth, and therefore cutting time is to be early advisable, in case there is freeze injury winter, height of seedling 15.2cm then, leading thread 0.4cm; The hard branch of ginkgo gets final product pumping shoot in seedbed, and young sprout germination rate reaches 80%, and after nursery stock transplanting, because seedling-slowing stage is long, the amount of taking root is little then;
(6) stump rejuvenation, stump instrument is shrub and hedge trimmer, and the stump time transplants when latter 1 year, base portion have new life dry again and carries out in cuttage seeding, and while noting stump, crops for rotation want level and smooth, without splitting a batch breach, stubble height 3-5cm; Common 1 strain cuttage seeding is germinated the multiple dry of 3-5 growth vertically upward after stump, and doing again number with age growth obviously increases, and sets up improved seeds cutting orchard by this approach, scion or the cuttings breeding elimination position effect of utilizing this cutting orchard to obtain.
2. a kind of method that overcomes ginkgo vegetative propagation position effect according to claim 1, is characterized in that, the Best Times of described step (5) acclimatization and transplants is epicormic branch cuttage after 45 days, and cuttage is after 35 days.
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CN104126419B (en) * 2014-07-18 2016-05-18 山东农业大学 Ginkgo ancient tree choiceness breeding garden method for building up
CN105746280A (en) * 2016-03-15 2016-07-13 云南洪尧园林绿化工程有限公司 Ginkgo nursery-grown plant stumping and rejuvenating method
CN106105761A (en) * 2016-08-11 2016-11-16 施秉县佳禾苗木花卉农民专业合作社 A kind of cottage method of mountain region, plateau, subtropical zone ginkgo
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