CN105815211A - Method for improving self-incompatibility Wuta-tsai hybridization efficiency - Google Patents
Method for improving self-incompatibility Wuta-tsai hybridization efficiency Download PDFInfo
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- CN105815211A CN105815211A CN201610175595.3A CN201610175595A CN105815211A CN 105815211 A CN105815211 A CN 105815211A CN 201610175595 A CN201610175595 A CN 201610175595A CN 105815211 A CN105815211 A CN 105815211A
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- wuta
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- Developmental Biology & Embryology (AREA)
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a method for improving self-incompatibility Wuta-tsai hybridization efficiency and belongs to the technical field of gardening. The method is mainly aimed at solving the problem that labor and time are wasted and work efficiency is low in artificial Wuta-tsai hybridization. Wuta-tsai hybridization efficiency is improved through isolation, reasonable composition, bag sleeving, unfilled seed removal and the like. The method has the advantages of being easy to operate, low in cost, good in effect and the like.
Description
Technical field
The present invention relates to a kind of method improving self-incompatibility Wuta-tsai hybridization efficiency, belong to technical field of gardening.
Background technology
Wuta-tsai is a mutation of Cruciferae Brassica genus Vegetable Crops of Brassica Plantula Brassicae chinensis subspecies, originates in China, is mainly distributed on the Changjiang river, Basin of Huaihe River, the cultivation history of existing nearly one thousand years, the Song dynasty, the Ming Dynasty relevant document in have been described.Wuta-tsai is more universal in middle and lower reach of Yangtze River cultivation, can whole year production, four seasons supply.Wuta-tsai tolerance to cold is relatively strong, does not fear frost, and along area, Huaihe River energy open field soil, after frost and snow, its quality is the tenderest, and fiber is few, and food flavor is delicious, and more preferably, be considered in Chinese cabbage is top grade for quality, has the good reputation of " twelfth month crow dish match Carnis caprae seu ovis ".According to surveying and determination, edible part moisture content about 92g, protein 1.56 ~ 3g, the reducing sugar 0.80g of every 100g Wuta-tsai, fat 0.4g, cellulose 2.63g, vitamin C 43 ~ 75mg, carotene 1.52 ~ 3.5mg, vitamin B10.02mg, vitamin B20.14mg, potassium 382.6mg, sodium 42.6mg, calcium 154 ~ 241mg, phosphorus 46.3mg, copper 0.111mg, manganese 0.319mg, selenium 2.39mg, ferrum 1.25 ~ 3.30mg, zinc 0.306mg, strontium 1.03mg, it is because of rich in ascorbic acid, carotene and calcareous mineral salts, it is referred to as " vitamin " dish, cools due to its season of growth weather again, pest and disease damage seldom occurs, or need not seldom use pesticide, therefore be pollution-free vegetable.It addition, " food book on Chinese herbal medicine " carries: Wuta-tsai is sweet, flat, nontoxic, can laxation, the five internal organs soothing the liver, sharp.Often eating Wuta-tsai and can prevent constipation, strengthen human body disease resistance ability, skin care is vigorous and graceful.People increase the research dynamics to Wuta-tsai in recent years, but Wuta-tsai carries out the utilization of selection cross and cenospecies, and its workload is big, time-consuming, how to improve self-incompatibility Wuta-tsai hybridization efficiency urgently to be resolved hurrily.
The invention discloses a kind of method improving self-incompatibility Wuta-tsai hybridization efficiency, belong to technical field of gardening.It manually carries out mainly for Wuta-tsai hybridizing inefficient feature time-consuming, worked, by isolating, rationally assembling, field planting, bagging, rocks, gathers in the crops, removes flat grain totally 7 steps and improve Wuta-tsai hybridization efficiency.The present invention has simple to operate, low cost, the feature such as effective.
Summary of the invention
It is an object of the invention to provide a kind of method improving self-incompatibility Wuta-tsai hybridization efficiency, it is possible to resolve the shortcoming that self-incompatibility Wuta-tsai hybridization efficiency is low, time-consuming, the utilization for cross-breeding and cenospecies is provided convenience.
Technical scheme
The present invention is achieved in that
nullThe enforcement of a kind of method improving self-incompatibility Wuta-tsai hybridization efficiency: on the premise of utilizing the isolation of canopy room with other Cruciferae plant or carry out isolation by distance，The self-incompatibility Wuta-tsai of pre-preparation hybridization is rationally assembled，Carry out nursery in good time，In seedling 4-6 leaf phase field planting，Two strain Wuta-tsais of self-incompatibility pre-preparation hybridized during field planting are planted in together，Spacing 15-30cm is before Wuta-tsai will be bloomed，3-6 small bamboo pole is set up around two strain Wuta-tsais，Downwards two strain Wuta-tsais are nested together with fly net set bag mouth more than 60 mesh，With soil, bag base opening is compacted，Prevent the interference of external pollen，Post flowering periodically rocks fly net bagging and Wuta-tsai plant at the florescence，Promote pollen diffusion，Improve the two mutual pollination rate of strain in mesh bag，During seed maturity, the two strain Wuta-tsais to self-incompatibility carry out individual plant results，Remove flat grain，To reject the seed that respective selfing is tied，Other management generally rule.
Beneficial effect
The invention discloses a kind of method improving self-incompatibility Wuta-tsai hybridization efficiency, belong to technical field of gardening.The present invention uses itself and other Cruciferae plant should carry out canopy room or isolation by distance, the self-incompatibility Wuta-tsai preparing hybridization is rationally assembled, it is planted in together, with fly net baggings more than 60 mesh, two strain Wuta-tsais are nested together before will blooming, prevent insecticide from passing in and out, effectively prevent the interference of external pollen, Post flowering periodically rocks fly net bagging at the florescence, promote pollen diffusion, improve the two mutual pollination rate of strain in mesh bag, individual plant results remove flat grain, to reject the seed that respective selfing is tied, isolated by science, self-incompatibility Wuta-tsai rationally assembles, field planting, fly net bagging, florescence periodically rocks, individual plant is gathered in the crops, remove flat grain to improve Wuta-tsai hybridization efficiency.Showing according to result of the test, use this law to carry out self-incompatibility Wuta-tsai hybridization, it is made without artificial all pollination, and Cross fertile rate is high, and the seed of cross-fertile is full, and its effect is significantly better than the control treatment being provided without this law.The present invention has the advantage that 1) low cost, 2) effective, 3), simple to operate.The present invention provides reliable technical scheme for improving self-incompatibility Wuta-tsai hybridization efficiency.
Detailed description of the invention
Below by detailed description of the invention, the present invention is described in further detail.
In following embodiment, method therefor is conventional method if no special instructions.
1. implementation:
1. the enforcement of the method improving self-incompatibility Wuta-tsai hybridization efficiency: the self-incompatibility Wuta-tsai of pre-preparation hybridization was rationally assembled by we in recent years, general and 9-10 month carry out nursery, the field planting when the seedling 4-6 leaf phase, two strain Wuta-tsais of self-incompatibility pre-preparation hybridized during field planting are planted in together, spacing 20cm, and canopy room or isolation by distance should be carried out with other Cruciferae plant.Before will blooming March in the coming year two strain Wuta-tsai peripheries insert bamboo pole supporters and with 60 mesh more than 80cm*100cm specification fly net overlap bag mouth downwards two strain Wuta-tsais are nested together, bottom soil is by real for bag mouth envelope, prevent insecticide from passing in and out, Post flowering periodically rocks fly net bagging at the florescence, to promote pollen diffusion, improve the two mutual pollination rate of strain；Individual plant results remove flat grain, other management generally rule, are made without artificial all pollination through this method of application attestation, and Cross fertile rate is high, and the seed of cross-fertile is full, is effectively increased the hybridization efficiency of self-incompatibility Wuta-tsai.
Claims (2)
1. the method improving self-incompatibility Wuta-tsai hybridization efficiency, it is characterised in that described method includes isolating, rationally assembles, field planting, bagging, rock, gather in the crops, remove flat grain totally 7 steps.
A kind of method improving self-incompatibility Wuta-tsai hybridization efficiency, it is characterised in that the particular content of 7 described steps is:
Isolation: refer to utilize the isolation of canopy room with other Cruciferae plant or carry out isolation by distance；
Rationally assemble: the self-incompatibility Wuta-tsai of pre-preparation hybridization is rationally assembled；
Field planting: in seedling 4-6 leaf phase field planting, two strain Wuta-tsais of self-incompatibility pre-preparation hybridized during field planting are planted in together, spacing 15-30cm；
Bagging: before Wuta-tsai will be bloomed, sets up 3-6 small bamboo pole around two strain Wuta-tsais, is nested together by two strain Wuta-tsais downwards with fly net set bag mouth more than 60 mesh, bag base opening is compacted with soil；
Rock: Post flowering periodically rocks fly net bagging and Wuta-tsai plant at the florescence；
Results: during seed maturity, the two strain Wuta-tsais to self-incompatibility carry out individual plant results；
Remove flat grain: remove the flat grain in sowing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610175595.3A CN105815211A (en) | 2016-03-26 | 2016-03-26 | Method for improving self-incompatibility Wuta-tsai hybridization efficiency |
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| CN201610175595.3A CN105815211A (en) | 2016-03-26 | 2016-03-26 | Method for improving self-incompatibility Wuta-tsai hybridization efficiency |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106258941A (en) * | 2016-09-21 | 2017-01-04 | 韶关学院 | The selection-breeding of a kind of Wuta-tsai and propagation method |
| CN116616170A (en) * | 2023-07-14 | 2023-08-22 | 安徽省华禾种业有限公司 | Breeding method for maintaining variety diversity of Wuta-tsai |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103461100A (en) * | 2013-08-26 | 2013-12-25 | 安徽省农业科学院园艺研究所 | Purple Wuta-tsai breeding method |
| CN104472343A (en) * | 2014-12-17 | 2015-04-01 | 安徽农业大学 | Breeding method for the self-incompatible line of brassica campestris L. ssp. chinensis (L.) makino var. rosularis tsen et lee |
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2016
- 2016-03-26 CN CN201610175595.3A patent/CN105815211A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103461100A (en) * | 2013-08-26 | 2013-12-25 | 安徽省农业科学院园艺研究所 | Purple Wuta-tsai breeding method |
| CN104472343A (en) * | 2014-12-17 | 2015-04-01 | 安徽农业大学 | Breeding method for the self-incompatible line of brassica campestris L. ssp. chinensis (L.) makino var. rosularis tsen et lee |
Non-Patent Citations (2)
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| 赵利民: "大白菜杂交制种技术规程", 《种子世界》 * |
| 陈杏禹等主编: "《蔬菜种子生产技术》", 31 August 2011 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106258941A (en) * | 2016-09-21 | 2017-01-04 | 韶关学院 | The selection-breeding of a kind of Wuta-tsai and propagation method |
| CN116616170A (en) * | 2023-07-14 | 2023-08-22 | 安徽省华禾种业有限公司 | Breeding method for maintaining variety diversity of Wuta-tsai |
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Application publication date: 20160803 |