CN108012921A - A kind of selection of atropurpureus spy rice - Google Patents

A kind of selection of atropurpureus spy rice Download PDF

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Publication number
CN108012921A
CN108012921A CN201810047050.3A CN201810047050A CN108012921A CN 108012921 A CN108012921 A CN 108012921A CN 201810047050 A CN201810047050 A CN 201810047050A CN 108012921 A CN108012921 A CN 108012921A
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rice
atropurpureus
parent
selection
generation
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CN201810047050.3A
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Chinese (zh)
Inventor
吴殿星
徐艺如
黄婧
任意
任一意
诸方宁
曾嘉乐
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Zhejiang University ZJU
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Zhejiang University ZJU
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Priority to CN201810047050.3A priority Critical patent/CN108012921A/en
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • A01H1/08—Methods for producing changes in chromosome number

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Molecular Biology (AREA)
  • Cultivation Of Plants (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

The invention discloses a kind of selection of atropurpureus spy rice, this method includes：Selection atropurpureus blade rice is parent A, husk and kind skin are that atropurpureus rice is parent B, and parent A and parent B is hybridized, obtains F1For seed；Plant single cross F1In generation, be returned with parent A, obtains BC1F1For seed；Plant BC1F1In generation, double rate is built using Anther Culture, seed is harvested by single plant；Plant DH1In generation, select the strain that blade, husk, kind skin are atropurpureus；Plant DH2~DH3In generation, select japonica rice type, rice blast comprehensive resistance index<9 grades of excellent strain；Plant DH4In generation, selection and breeding blade, husk and kind skin are atropurpureus, rice blast comprehensive resistance index<9 grades of sizing rice, that is, obtain atropurpureus spy's rice.Atropurpureus is all presented in the new rice variety of selection and breeding of the present invention, blade and husk, and ornamental value is stronger, and combines the high added value and deep processing purposes of atropurpureus special type rice, is conducive to agriculture trip combination.

Description

A kind of selection of atropurpureus spy rice
Technical field
The present invention relates to rice breeding technology field, more particularly to a kind of selection of atropurpureus spy rice.
Background technology
With flourishing for China's ecological agriculture and rural area synthesis, agricultural production and agricultural are gone sightseeing with reference to being increasingly becoming One of new highlight of Space For Beauity, becomes production, life, the organic combination of ecology.Served as theme with local leading industry, by section Skill is used for agricultural production process and intention development of agricultural products with theory intention, has promoted the interactive hair of tourism, culture and agricultural Exhibition, makes Creative Agriculture become the new highlight of " growth of agricultural efficiency, increasing peasant income ".
Currently used for the rice of Creative Agriculture, predominantly yellow, the rice of purple and green blade, there are intention kind compared with Single, bright coloured silk is celebrating not enough, ornamental value is not strong, while external color is focused on, ignores work(of the product as food and cash crops Can characteristic.
The content of the invention
It is an object of the invention to provide a kind of selection of atropurpureus spy rice, this method selection and breeding obtain purple black Color spy not only has an ornamental value with rice, but also seed has a higher nutritive value, and the content of vitamin A and zinc is shown in seed Work is higher than parent.
A kind of selection of atropurpureus spy rice, it is characterised in that including：
(1) select blade to be used as parent A for the rice of atropurpureus, husk with to plant skin be the rice of atropurpureus as parent B, parent A and parent B is hybridized, and obtains F1For seed；
(2) F is planted1In generation, be returned with parent A, obtains BC1F1For seed；
(3) BC is planted1F1In generation, using Anther Culture mode, double rate is built, DH is harvested by single plant1Generation kind Son；
(4) DH is planted1In generation, select and remain the strain that blade is atropurpureus, selecting and remain husk in the maturity period is in vegetative growth stage The strain of atropurpureus, carries out selecting and remain the strain that kind of skin is atropurpureus during heat treatment after harvesting, harvests DH2For seed；
(5) DH is planted2In generation, select japonica rice type strain, harvests DH3For seed；
(6) DH is planted3In generation, select rice blast resistance composite index<9 grades of strain, harvests DH4For seed；
(7) DH is planted4Generation, selection and breeding blade, husk and kind skin are the sizing rice of atropurpureus and non-high sense rice blast, are obtained To atropurpureus spy's rice.
In the present invention, the approved product, sizing rice refer to atropurpureus spy's rice.
Preferably, the parent A is the black treasure of Zhejiang University.
" the black treasure of Zhejiang University " is the extraordinary black glutinous rice rice of Zhejiang University's selection and breeding, is purchased from agricultural college of Zhejiang University.The rice For japonica rice, there is atropurpureus blade and yellow husk, be common rice, middle sense rice blast.
Preferably, the parent B is 9311-br-01.
" 9311-br-01 " is that Zhejiang University uses the husk of the cultivation of the radioinduction of cobalt -60 9311 and plants skin all for atropurpureus Special Rices, be purchased from agricultural college of Zhejiang University.The rice is long-grained nonglutinous rice, and blade is normal green, and husk is atropurpureus, rice kind Skin is atropurpureus, height sense rice blast.
Preferably, the parent A is the black treasure of Zhejiang University；The parent B is 9311-br-01.
The black precious, 9311-br-01 of Zhejiang University is selected to carry out hybridization combo, atropurpureus spy's rice of selection and breeding, is atropurpureus leaf Piece, atropurpureus husk, the efficient combination of atropurpureus, not only have very strong ornamental value, it is atropurpureus, also have very high battalion Value is supported, the application prospect with the exploitation series of products such as atropurpureus and black rice wine.
Preferably, in step (5), the selection of the japonica rice type strain is：To DH2Carried out for plant at short-day Reason, using the parent A for not carrying out short-day sunshine treatment as control, selects the strain that heading stage shifts to an earlier date more than 10 days compared with the control.
Further preferably, the short-day sunshine treatment is 5 points of every afternoon to 8 points of the next morning by DH2Moved to for plant In dark surrounds, coprocessing 15 days.
Preferably, in step (6), using standard GB/T/T 15790-2009 as according to identification DH3For the rice blast of plant Sick resistance syndrome index.
Compared with prior art, beneficial effects of the present invention are：
(1) present invention is using the rice of atropurpureus blade as parent A, using the rice of atropurpureus husk and kind skin as parent B, Selection and breeding obtain atropurpureus spy's rice, and not only atropurpureus is all presented in blade and husk to the rice, has stronger ornamental value, and Vitamin and all kinds of trace elements are rich in seed, the content of wherein vitamin A and zinc is higher and is significantly higher than parent.
(2) the atropurpureus spy of selection and breeding of the present invention is with the high added value and deep processing purposes of rice combination atropurpureus special type rice, The technical need of Creative Agriculture development is more in line with, contributes to agriculture to be combined with tourism.
(3) present invention carries out selection and breeding using the strategy of the japonica rice parent backcrossing of tool atropurpureus blade, is not only advantageous to spend training Culture, selection obtain quality and are more susceptible to the japonica rice that urban consumption person is welcome, and are remarkably improved selection offspring's atropurpureus The segregation ratio of blade, realizes and polymerize husk color and rice seed coat colour on the basis of color leaf fancy points, to be special with establishing base Plinth.
(4) present invention is conducive to product and meets disappearing for vast urban consumption person using selection and breeding japonica rice type as important breeding objective Take demand, more preferably realize high-quality synergy.
Embodiment
1st, breeding material：
(1) the black treasure of Zhejiang University is parent A.The black treasure of Zhejiang University is the extraordinary black glutinous rice rice of Zhejiang University's selection and breeding, is purchased from Zhejiang Agricultural college of university.The rice is japonica rice, has atropurpureus blade and yellow husk, is common rice, middle sense rice blast.
(2) 9311-br-01 is parent B.9311-br-01 is that Zhejiang University is cultivated using the radioinduction of cobalt -60 9311 Husk and kind skin are all the Special Rices of atropurpureus, are purchased from Zhejiang University.The rice is long-grained nonglutinous rice, and blade is normal green, husk For atropurpureus, rice kind skin is atropurpureus, height sense rice blast.
2nd, a kind of atropurpureus spy is with the Breeding Process of rice：
(1) 2012 year spring, Hainan Lingshui, with Zhejiang University it is black treasure for parent A, make female parent, respectively with parent B (9311-br- 01) hybridize, obtain F1(the black treasure/9311-br-01 of Zhejiang University) is for 7, seed；
(2) 2012 year summer and autumn, Zhejiang Hangzhou, plants F1(the black treasure/9311-br-01 of Zhejiang University) for 7 plants of plant, with parent A The black precious backcrossing of Zhejiang University, obtains BC1F1(the black treasure/9311-br-01 of black treasure // Zhejiang University of Zhejiang University) is for 36 plants of plant；
(3) 2013 year spring, Hainan Lingshui, plantation backcrossing BC1F1(the black treasure/9311-br-01 of black treasure // Zhejiang University of Zhejiang University) generation 36 plants, using Anther Culture mode, build doubled haploid (DH) colony；
(4) 2013 year summer and autumn, Zhejiang Hangzhou, by single-strain planting and harvests DH0For seed, structure DH 532 (DH of system1Generation Seed)；
In (5) 2014 year spring, Hainan Lingshui, plants DH1Generation, in vegetative growth stage selection atropurpureus blade strain 167 It is a, it is atropurpureus strain 41 in maturity period selection husk, it is the strain of black to carry out selection kind skin during heat treatment after harvesting It is 23；
(6) 2014 year summer and autumn, Zhejiang Hangzhou, plants DH2In generation, existed using the transplanting of daily 8 points of the next morning of at 5 in afternoon In dark surrounds, continuous processing 15 days, have selected 15 of photosensitive japonica rice type excellent strain；
(7) 2015 year summer and autumn, Zhejiang Hangzhou, plants DH3In generation, by rice blast selection and breeding, select rice blast resistance synthesis to refer to Number<9 grades 2 (using standard GB/T/T 15790-2009 as foundation)；
(8) 2017 year summer and autumn, Zhejiang Hangzhou, plants DH4In generation, selection and breeding blade, husk, rice kind skin are that atropurpureus is taken into account The sizing rice of rice blast resistance, that is, obtain atropurpureus spy's rice.
Some characteristic indexs record is as shown in table 1 in each generation.
Situation table is recorded in the screening of each generation character determination of table 1
The leading indicator of the sizing rice of the present embodiment selection and breeding is：Japonica rice, atropurpureus blade, atropurpureus husk, black rice, has Effect has polymerize the merit of three parents, and has certain rice blast resistance.And the black treasure of parent A Zhejiang University is japonica rice, atropurpureus Blade, yellow husk, common rice, middle sense rice blast；Parent B9311-br-01 is long-grained nonglutinous rice, has normal green blade, and husk is Atropurpureus, rice kind skin are atropurpureus, height sense rice blast.
It is determined after above-mentioned screening obtains approved product, and to its a variety of nutritive index, approved product and its parent This index determining is as a result, be shown in Table 2.
The assay method of each index is as follows in table 2：
Lipid：GB/T5009.6-2003；Protein：GB5009.5-2010；
Vitamin A：GB/T5009.82-2003；Vitamin B1：GB/T5009.84-2003；
Vitamin B2：GB/T5009.85-2003；Vitamin E：GB/T5009.82-2003；
Calcium：GB/T5009.92-2003；Iron：GB/T5009.90-2003；Zinc：GB/T5009.14-2003；
The main nutrient composition of the black treasure of 2 Zhejiang University of table, good Zhejiang rice No. 1 and approved product compare
It is the advantages of approved product polymerize 2 parents, rich in the nutritional ingredient of approved product from the data in table 2 Fatty, protein, vitamin, micronutrient element, wherein, VAIt is higher than parent with the content of zinc.

Claims (7)

  1. A kind of 1. selection of atropurpureus spy rice, it is characterised in that including：
    (1) selecting the rice that blade is atropurpureus, husk and kind skin are the rice of atropurpureus as parent B, general as parent A Parent A and parent B hybridizes, and obtains F1For seed；
    (2) F is planted1In generation, be returned with parent A, obtains BC1F1For seed；
    (3) BC is planted1F1In generation, using Anther Culture mode, double rate is built, DH is harvested by single plant1For seed；
    (4) DH is planted1In generation, select and remain the strain that blade is atropurpureus, it is atropurpureus that husk is selected and remain in the maturity period in vegetative growth stage Strain, after harvesting carry out heat treatment when select and remain kind of skin be atropurpureus strain, harvest DH2For seed；
    (5) DH is planted2In generation, select japonica rice type strain, harvests DH3For seed；
    (6) DH is planted3In generation, select rice blast resistance composite index<9 grades of strain, harvests DH4For seed；
    (7) DH is planted4Generation, selection and breeding blade, husk and kind skin are the sizing rice of atropurpureus and non-high sense rice blast, obtain purple Black spy's rice.
  2. 2. selection according to claim 1, it is characterised in that the parent A is the black treasure of Zhejiang University.
  3. 3. selection according to claim 1, it is characterised in that the parent B is 9311-br-01.
  4. 4. selection according to claim 1, it is characterised in that the parent A is the black treasure of Zhejiang University；The parent B is 9311-br-01.
  5. 5. selection according to claim 1, it is characterised in that in step (5), the selection and breeding side of the japonica rice type strain Method is：To DH2Short-day sunshine treatment is carried out for plant, using the parent A for not carrying out short-day sunshine treatment as control, is selected compared with the control Heading stage shifts to an earlier date the strain of more than 10 days.
  6. 6. selection according to claim 5, it is characterised in that the short-day sunshine treatment is 5 points of every afternoon to the Two days 8 a.m.s are by DH2Moved to for plant in dark surrounds, coprocessing 15 days.
  7. 7. selection according to claim 1, it is characterised in that in step (6), with standard GB/T/T 15790- 2009 be according to identification DH3For the rice blast resistance composite index of plant.
CN201810047050.3A 2018-01-18 2018-01-18 A kind of selection of atropurpureus spy rice Pending CN108012921A (en)

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109042294A (en) * 2018-08-10 2018-12-21 浙江大学 A kind of selection of red endosperm rice
CN109042289A (en) * 2018-07-17 2018-12-21 浙江大学 A kind of selection of the high anthocyanidin rice of black endosperm
CN109042290A (en) * 2018-07-17 2018-12-21 浙江大学 A kind of polymerization selection of black endosperm rice
CN113994886A (en) * 2021-11-19 2022-02-01 浙江大学 Breeding method of ornamental and edible hybrid rice sterile line
CN113994887A (en) * 2021-11-19 2022-02-01 浙江大学 Breeding method of high-ornamental high-nutrition hybrid rice
CN114402993A (en) * 2021-12-27 2022-04-29 浙江大学 Method for breeding high-quality color hybrid rice

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CN102524047A (en) * 2011-12-26 2012-07-04 杨玉梁 Cultivation and product production method of natural vitamin A containing fragrant rice type photo-thermo-sensitive sterile line and restorer line of special rice
CN106665337A (en) * 2017-03-20 2017-05-17 杭州中泽生物科技有限公司 Rice breeding method

Patent Citations (2)

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Publication number Priority date Publication date Assignee Title
CN102524047A (en) * 2011-12-26 2012-07-04 杨玉梁 Cultivation and product production method of natural vitamin A containing fragrant rice type photo-thermo-sensitive sterile line and restorer line of special rice
CN106665337A (en) * 2017-03-20 2017-05-17 杭州中泽生物科技有限公司 Rice breeding method

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Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109042289A (en) * 2018-07-17 2018-12-21 浙江大学 A kind of selection of the high anthocyanidin rice of black endosperm
CN109042290A (en) * 2018-07-17 2018-12-21 浙江大学 A kind of polymerization selection of black endosperm rice
CN109042289B (en) * 2018-07-17 2021-08-03 浙江大学 Breeding method of black endosperm high-anthocyanin rice
CN109042290B (en) * 2018-07-17 2021-08-10 浙江大学 Polymerization breeding method of black endosperm rice
CN109042294A (en) * 2018-08-10 2018-12-21 浙江大学 A kind of selection of red endosperm rice
CN109042294B (en) * 2018-08-10 2021-06-29 浙江大学 Breeding method of red endosperm rice
CN113994886A (en) * 2021-11-19 2022-02-01 浙江大学 Breeding method of ornamental and edible hybrid rice sterile line
CN113994887A (en) * 2021-11-19 2022-02-01 浙江大学 Breeding method of high-ornamental high-nutrition hybrid rice
CN113994886B (en) * 2021-11-19 2022-08-19 浙江大学 Breeding method of ornamental and edible hybrid rice sterile line
CN114402993A (en) * 2021-12-27 2022-04-29 浙江大学 Method for breeding high-quality color hybrid rice

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Application publication date: 20180511