CN105519431A - Efficient test cross breeding method of maize inbred line combining ability - Google Patents
Efficient test cross breeding method of maize inbred line combining ability Download PDFInfo
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- CN105519431A CN105519431A CN201610099205.9A CN201610099205A CN105519431A CN 105519431 A CN105519431 A CN 105519431A CN 201610099205 A CN201610099205 A CN 201610099205A CN 105519431 A CN105519431 A CN 105519431A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
The invention provides an efficient test cross breeding method of maize inbred line combining ability. The method includes: adopting a plant test cross method, only retaining single plants with high combining ability, growing next generations in head progeny rows, and then performing plant test cross till the head progeny rows are stable; secondly, according to a conventional breeding procedure, matching cross combination, performing multi-site test, regional test and the like, and finally breeding a new crossbreed to finish the whole breeding procedure. Compared with the conventional technique, the method has the advantages that the inbred line combining ability can be maintained at a high level, measurement accuracy is high, and efficiency of breeding the inbred lines with high combining ability can be increased by more than 50%. The problem of blindness in combining-ability test cross of the conventional technique is solved, accuracy in breeding the inbred lines with high combining ability is improved, and a foundation is laid for improving efficiency of commercialized maize breeding.
Description
Technical field
The present invention relates to crop breeding field, specifically a kind of efficient test cross breeding method of Combing Ability of Corn Inbred Line.
Background technology
Corn is the different flower of monoecism, amphigenetic crop.The main path of corn yield increasing utilizes Heterosis of Maize Hybrid.The emphasis of corn breeding work cultivates corn hybrid seed exactly.Selecting and breeding corn inbred line is the basis of cultivating corn hybrid seed; By the preparation of inbred line intermolecular hybrid Maize Combination, selecting and breeding corn new hybrid.So the technology of selecting and breeding corn inbred line is the core technology of corn breeding.The breeding technique of traditional corn inbred line, must through the selfing screening in many generations for many years, after inbred line is stable, then hybridizes with Testers and prepares test cross kind, carry out combining ability test, and then select Elite inbred and crossbreed.But, adopt which kind of coordinate force test cross method, the inbred line of high-combining ability can be selected fast and accurately, be only and assembly can go out the key of strong superior hybrid crosses.
The conventional method measuring Combing Ability of Corn Inbred Line mainly contains: (1) method of inbred-variety cross: be make Testers with common variety, variety difference or synthetic cross variety, be early female parent with Testers during generation (before S2 generation) test, tested selfing head progeny row is male parent.In S2 generation, when testting, usually using tested inbred line as female parent, Testers was male parent later.Also test cross can be carried out by father's many maternals formula in isolated area.According to test cross kind Identification result next year, the selfing head progeny row that final election is tested.(2) combined techniques is measured: be the method that mensuration coordinate force carries out together with apolegamy Combination nova.Newly select inbred line and existing Elite inbred to carry out bagging hybridization, after Yield compari@is had discovered some clues, can continue composite, carry out multispots trial early.Facts have proved, be thisly measured combined techniques, survey limit, limit use, can accelerate breeding generation, and breeding process and the time limit are shortened dramatically, and accelerates the selection and popularization speed of corn hybrid seed.
The defect of above-mentioned these two kinds of methods existing is, the pollen mixture in tested system selfing head progeny row and Testers is all adopted to prepare test cross kind, the height of test cross kind output, only reflects the integral level of tested system selfing head progeny row coordinate force, is difficult to the difference distinguishing coordinate force between individual plant in tested system selfing head progeny row.Because early generation is separation, unstable generation in tested system, and between individual plant, the height of coordinate force is discrepant.Can survey limit, limit use although be measured combined techniques, can breeding generation be accelerated, breeding process and the time limit are shortened dramatically.But tested system effectively can not be selected early for high-combining ability individual plant in head progeny row, likely cause high-combining ability individual plant be eliminated because of reasons such as variable rate technology or lose.So, select accuracy rate low, weak effect.After often causing selection for many years, selfing head progeny row stabilizes, and economical character is pretty good, and coordinate force is but not high, is difficult to be bred as strong superior hybrid crosses, has to eliminate.Cause human and material resources and waste of time.
Summary of the invention
The present invention is intended to for the deficiencies in the prior art, there is provided a kind of efficient test cross breeding method of Combing Ability of Corn Inbred Line, compared with conventional art, inbred line coordinate force of the present invention can maintain the lower continuity of high level, mensuration accuracy rate is high, and the efficiency of seed selection high-combining ability inbred line can improve more than 50%.Solving the blindness of conventional art coordinate force test cross, improve the accuracy of seed selection high-combining ability inbred line, laying a good foundation for improving corn Hybrid breeding in commercial system efficiency.
Technical solution of the present invention is as follows, the efficient test cross breeding method of Combing Ability of Corn Inbred Line, adopts strain test cross method, only retains the high individual plant of coordinate force, and of future generation continuation plants into head progeny row, then to strain test cross, until head progeny row is stablized.
Specifically, early generation is as tested system; General use mixes excellent group clearly, and Testers is made in pedigree source clearly conventional system; Tested system half pollen is used for selfing, each head progeny row selfing 10 strain, No. 1-10, numbering of listing; Meanwhile, get second half pollen and Testers hybridizes 3-4 fringe, and numbering of listing, mixed de-after the hybridization fringe results of identical numbering, next season, as the plantation of test cross kind, carries out the test of test cross kind Yield compari@; According to variable rate technology and the yield level of test cross kind, determine the choice of corresponding numbering selfing fringe; The individual plant that coordinate force is high, of future generation continuation plants into head progeny row, then to strain test cross, the individual plant that coordinate force is not high, will be eliminated; Each generation only retains 10%-20% high-combining ability individual plant, until head progeny row is stablized; Then, the conveniently procedure of breeding, assembly hybrid combination, carries out multispots trial, regional test etc., is finally bred as new hybrid, completes the whole procedure of breeding.
Of the present invention to strain test cross method be generation tested morning (S2 generation) be individual plant selfing add for time half pollen for selfing, and to number respectively; Second half pollen and Testers are hybridized simultaneously, preparation test cross kind, and number respectively; Next season, according to the variable rate technology of test cross kind and yield level, determines the choice of corresponding numbering selfing fringe.The individual plant that coordinate force is high, of future generation continuation plants into head progeny row, then to strain test cross.The individual plant that coordinate force is not high, will be eliminated.Each generation only retain 10% ~ 20% high-combining ability individual plant, until head progeny row stablize.
Combing Ability of Corn Inbred Line of the present invention to strain test cross method as shown in Figure 1.The coordinate force of the tested system of this method can keep the lower continuity of high level, and measure accuracy rate high, the efficiency of seed selection high-combining ability inbred line can improve more than 50%.Solve the blindness of conventional method coordinate force test cross.Both effectively can reduce Breeding Scale, turn improve efficiency of selection.
The method of hybridization is artificial pollination hybridization, and early generation is the morning of the head progeny row from generation to generation of Breeding of Inbred Lines, is also tested system, generally presses the plantation of head progeny row order.Testers be with tested be the inbred line or colony of hybridizing, general clear and definite with assorted excellent group, pedigree source is conventional system clearly.For ensureing tested system and Testers flower synchronization, successful formulation test cross kind, generally according to the sowing time of the florescence adjustment Testers of tested system.Bagging is wanted to isolate before Testers fruit ear reels off raw silk from cocoons.Before preparation test cross kind 1 ~ 2 day, be the seed purity and the quantity that ensure test cross kind, generally Testers done emasculation and cut bract process.
Generation described morning (S2 generation) is when being individual plant selfing, numbering of listing, as: A-1, A-2 ..., A-10 etc.General each morning is for about head progeny row selfing 10 strain.
Generation described morning (S2 generation) is while individual plant selfing adds generation, gets half pollen with Testers is hybridized, and prepares test cross kind, and numbers respectively; Refer to that adopting early generation is individual plant pollen, half pollen is used for selfing, and second half pollen and Testers hybridize 3 ~ 4 fringes, and numbering of listing.Mixed de-after the hybridization fringe results of identical numbering, next season, as the plantation of test cross kind, carries out the test of test cross kind Yield compari@, namely surveys than test.
The described variable rate technology according to test cross kind and yield level, determine the choice of corresponding selfing fringe.Namely according to the variable rate technology of test cross kind and the height of yield level, whether the reservation of selfing fringe in its corresponding parent's head progeny row is determined.As in Fig. 1, if the variable rate technology of test cross kind (combination) B × A-3 is good, output is higher, and its corresponding tested system selfing fringe A-3 will retain, and of future generation continuation plants into head progeny row, then to strain test cross.And other selfing fringe A-1, A-2, A-4 of tested system are until A-10, all to eliminate.Each generation only retain 10% ~ 20% high-combining ability individual plant, until head progeny row stablize.
Described each generation only retain 10% ~ 20% high-combining ability individual plant, until head progeny row stablize.Refer to that each test cross only retains the selfing fringe of 10% ~ 20% from generation to generation, give over to seed of future generation, continue strain test cross.If there are 10 selfing fringes in tested system, the fringe that just reservation 2 is best, the next generation plants into head progeny row respectively, continues strain test cross.General after sending to for 5 ~ 6 generations, various economical character and coordinate force just basicly stable, then during assembling cross-fertilize seed, just can adopt pollen mixture assembly.
The present invention has following beneficial effect
(1) the mensuration accuracy rate of inbred line coordinate force is high, improves more than 80%, avoids the blindness of prior art;
(2) efficiency of seed selection high-combining ability inbred line can improve more than 50%;
(3) can effectively reduce Breeding Scale more than 30%, use manpower and material resources sparingly more than 20%.
Accompanying drawing explanation
Fig. 1 be Combing Ability of Corn Inbred Line of the present invention to strain test cross breeding method to strain test cross schematic diagram.
Fig. 2 be the coordinate force of the inventive method Breeding of Inbred Lines neat 3925 to strain test cross method schematic diagram, select G. eurycarpa Lx9311 to make Testers.
Embodiment
Further illustrate the present invention by embodiment, following examples are the concrete embodiment of the present invention, but embodiments of the present invention are by the restriction of following embodiment.
The method used in the present invention if no special instructions, is this area conventional method.
Test material used in following embodiment, reagent etc., if no special instructions, all can obtain from commercial channels.The each crop varieties (strain) used is the conventional kind (strain) used in breeding field, is assert or technical appraisement by country's authorization, can obtain or market purchasing at species bank.
Embodiment 1
1. choosing is basic material: (neat 319
2× neat 205) BC1, is make female parent with neat 319, hybridizes, produce F1 with neat 205; Do maternal to backcross with neat 319 with F1 again, produce first backcross generation, that is: (neat 319
2× neat 205) BC1.
2. early generation (S2 generation) is (tested system): (neat 319
2× neat 205) BC1, in continuous selfing 2 generation, produces (neat 319
2× neat 205) BC1S2, being early generation is (neat 319
2× neat 205) BC1S2 is also tested system.
3. Testers: select G. eurycarpa Lx9311 to make Testers.
With (neat 319
2× neat 205) BC1S2 seed selection Elite inbred---neat 3925, step is as follows:
Winter Hainan in 1999: as shown in Figure 2, tested system (neat 319
2× neat 205) BC1S2 lists numbering, and (neat 319
2× neat 205) BC1S2-1, (neat 319
2× neat 205) BC1S2-2, (neat 319
2× neat 205) BC1S2-3 ..., (neat 319
2× neat 205) BC1S2-10.Tested system half pollen is used for selfing, each head progeny row selfing 10 strain.Meanwhile, get second half pollen and Testers Lx9311 hybridizes 3-4 fringe, and the numbering Lx9311 that lists × (neat 319
2× neat 205) BC1S2-1 test cross kind (combination), Lx9311 × (neat 319
2× neat 205) BC1S2-2 test cross kind (combination) ..., Lx9311 × (neat 319
2× neat 205) BC1S2-10 test cross kind (combination).
Summer in 2000: respectively 3 pilots, plantation Lx9311 × (neat 319
2× neat 205) 10 test cross combinations such as BC1S2-1, compare with Nongda108, carry out the test of test cross kind Yield compari@.According to analyses such as yield result, only Lx9311 × (neat 319
2× neat 205) BC1S2-3 is than contrast kind of Nongda108 volume increase more than 10%, therefore retains (neat 319
2× neat 205) BC1S2-3 selfing fringe, as next test cross kind in season.
Winter Hainan in 2000: plantation (neat 319
2× neat 205) BC1S2-3; The 2nd time is undertaken to strain test cross again by the step of Fig. 2.
Summer calendar year 2001: respectively 3 pilots, plantation Lx9311 × (neat 319
2× neat 205) 10 test cross combinations such as BC1S2-3-1, compare with Nongda108, carry out the test of test cross kind Yield compari@.According to analyses such as yield result, only Lx9311 × (neat 319
2× neat 205) BC1S2-3-2 is than contrast kind of Nongda108 volume increase more than 10%, therefore retains (neat 319
2× neat 205) BC1S2-3-2 selfing fringe, as next test cross kind in season.
Winter calendar year 2001 Hainan: plantation (neat 319
2× neat 205) BC1S2-3-2; The 3rd time is undertaken to strain test cross again by the step of Fig. 2.
Summer in 2002: respectively 3 pilots, plantation Lx9311 × (neat 319
2× neat 205) 10 test cross combinations such as BC1S2-3-2-1, compare with Nongda108, carry out the test of test cross kind Yield compari@.According to analyses such as yield result, only Lx9311 × (neat 319
2× neat 205) BC1S2-3-2-1 is than contrast kind of Nongda108 volume increase more than 10%, therefore retains (neat 319
2× neat 205) BC1S2-3-2-1 selfing fringe, as next test cross kind in season.
Winter Hainan in 2002: plantation (neat 319
2× neat 205) BC1S2-3-2-1; The 4th is carried out to strain test cross again by the step of Fig. 2.
Summer in 2003: respectively 3 pilots, plantation Lx9311 × (neat 319
2× neat 205) 10 test cross combinations such as BC1S2-3-2-1-1, compare with Nongda108, carry out the test of test cross kind Yield compari@.According to analyses such as yield result, only Lx9311 × (neat 319
2× neat 205) BC1S2-3-2-1-3 is than contrast kind of Nongda108 volume increase more than 10%, therefore retains (neat 319
2× neat 205) BC1S2-3-2-1-3 selfing fringe, as next test cross kind in season.
Winter Hainan in 2003: plantation (neat 319
2× neat 205) BC1S2-3-2-1-3; The 5th is carried out to strain test cross again by the step of Fig. 2.
Summer in 2004: respectively 3 pilots, plantation Lx9311 × (neat 319
2× neat 205) 10 test cross combinations such as BC1S2-3-2-1-3-1, compare with Nongda108, carry out the test of test cross kind Yield compari@.According to analyses such as yield result, only Lx9311 × (neat 319
2× neat 205) BC1S2-3-2-1-3-1 is than contrast kind of Nongda108 volume increase more than 10%, therefore retains (neat 319
2× neat 205) BC1S2-3-2-1-3-1 selfing fringe.As next test cross kind in season.
Winter Hainan in 2004: plantation (neat 319
2× neat 205) BC1S2-3-2-1-3-1; Field finds that the proterties such as plant are basicly stable, therefore stops strain test cross.Mixing gathers (neat 319
2× neat 205) pollen of BC1S2-3-2-1-3-1, combines with Lx9311 preparing hybrid.
Meanwhile, (neat 319
2× neat 205) BC1S2-3-2-1-3-1 continuation selfing is expanded numerous.
Summer in 2005: respectively 5 pilots, plantation Lx9311 × (neat 319
2× neat 205) the crossbreed Yield compari@such as BC1S2-3-2-1-3-1 test, compares with Nongda108.Result of the test: Lx9311 × (neat 319
2× neat 205) BC1S2-3-2-1-3-1 has outstanding performance.(neat 319
2× neat 205) BC1S2-3-2-1-3-1 inbred line called after neat 3925, continues selfing and expands numerous.
Lx9311 × (neat 319
2× neat 205) BC1S2-3-2-1-3-1 crossbreed called after Shandong single 7045.
In the winter in 2005: be maternal with neat 3925, Lx9311 is male parent, continues single 7045 crossbreed in composite Shandong.
Within 2006, Beijing's corn variety prerun is participated in Shandong single 7045, and than contrast kind of Nongda108 volume increase 15.8%, volume increase reaches pole significance level.
Within 2007, Beijing's corn variety regional test is participated in Shandong single 7045, on average increases production 3.4% than contrast kind of a Nongda108; Participate in Beijing's Maize Production test the same year, on average increase production 3.7% than contrast kind of a Nongda108.
Within 2008, Beijing's corn variety regional test is participated in Shandong single 7045, on average increases production 12.1% than contrast kind of a Nongda108; Participate in Beijing's Maize Production test the same year, on average increase production 14.0% than contrast kind of a Nongda108.
Within 2009, by the authorization of the Crops in Beijing kind committee, authorization number is: capital examines beautiful 2009003.
Embodiment 2
In the winter in 2005: be maternal with neat 3925, yellow 572 be male parent, another crossbreed Shandong composite singly 7055.
Within 2006, Sichuan Province's corn new hybrid prerun is participated in Shandong single 7055,13 volume increase 12% more single than contrast kind of river.
Within 2007, Sichuan Province's corn new hybrid regional test is participated in Shandong single 7055, than contrast kind of river single 13 on average volume increase 8.7%.
Within 2008, Sichuan Province's corn new hybrid regional test is participated in Shandong single 7055, than contrast kind of river single 13 on average volume increase 8.0%.
Within 2009, Sichuan Province's corn new hybrid production test is participated in Shandong single 7055, than contrast kind of river single 13 on average volume increase 9.7%.
Within 2010, Shandong single 7055 is by the authorization of the variety of crops committee of Sichuan Province, authorization called after Shandong single 718, and authorization number is: river examines beautiful 2010006.
Embodiment 3
In the winter in 2010: be maternal with neat 3925, Shandong is 032 is male parent, composite another crossbreed Shandong single 7091.
Shandong single 7091 in 2011 is participated in Anhui Province's new varieties and is combined qualification.
2012 to 2013 participate in the examination of provinces and regions, Anhui.
Within 2014, participate in Anhui Province's production test.
Authorization in 2015, called after sky benefit blue or green 7096.
Claims (3)
1. an efficient test cross breeding method for Combing Ability of Corn Inbred Line, is characterized in that, adopts strain test cross method, only retains the high individual plant of coordinate force, and of future generation continuation plants into head progeny row, then to strain test cross, until head progeny row is stablized.
2. the efficient test cross breeding method of Combing Ability of Corn Inbred Line described in claim 1, is characterized in that, described as follows to strain test cross method: generation tested morning be individual plant selfing add for time half pollen for selfing, and to number respectively; Second half pollen and Testers are hybridized simultaneously, preparation test cross kind, and number respectively; Next season, according to the variable rate technology of test cross kind and yield level, determines the choice of corresponding numbering selfing fringe; The individual plant that coordinate force is high, of future generation continuation plants into head progeny row, then to strain test cross, each generation only retains high-combining ability individual plant, until head progeny row is stablized.
3. the efficient test cross breeding method of Combing Ability of Corn Inbred Line described in claim 2, it is characterized in that, concrete steps are as follows:
Early generation is as tested system; General use mixes excellent group clearly, and Testers is made in pedigree source clearly conventional system; Tested system half pollen is used for selfing, each head progeny row selfing 10 strain, No. 1-10, numbering of listing; Meanwhile, get second half pollen and Testers hybridizes 3-4 fringe, and numbering of listing, mixed de-after the hybridization fringe results of identical numbering, next season, as the plantation of test cross kind, carries out the test of test cross kind Yield compari@; According to variable rate technology and the yield level of test cross kind, determine the choice of corresponding numbering selfing fringe; The individual plant that coordinate force is high, of future generation continuation plants into head progeny row, then to strain test cross, the individual plant that coordinate force is not high, will be eliminated; Each generation only retains 10%-20% high-combining ability individual plant, until head progeny row is stablized; Then, the conveniently procedure of breeding, assembly hybrid combination, carries out multispots trial, regional test etc., is finally bred as new hybrid, completes the whole procedure of breeding.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106973785A (en) * | 2017-04-06 | 2017-07-25 | 漯河市农业科学院 | A kind of corn inbred line breeding method |
| CN109169253A (en) * | 2018-08-22 | 2019-01-11 | 贵州省旱粮研究所 | Method based on corn Suwan-lancaster unexpected mass incident self-mating system |
| CN109197578A (en) * | 2018-11-22 | 2019-01-15 | 山东省农业科学院玉米研究所（山东省农业科学院玉米工程技术研究中心） | A kind of selection of P crowds of corn inbred line Lx286 |
| CN113475331A (en) * | 2021-08-05 | 2021-10-08 | 张掖市易农农业科技有限公司 | Planting method for corn DH line combining ability identification |
| CN113647321A (en) * | 2021-07-20 | 2021-11-16 | 河北省农林科学院旱作农业研究所 | Efficient and rapid detection method for corn inbred line combining ability |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106973785A (en) * | 2017-04-06 | 2017-07-25 | 漯河市农业科学院 | A kind of corn inbred line breeding method |
| CN109169253A (en) * | 2018-08-22 | 2019-01-11 | 贵州省旱粮研究所 | Method based on corn Suwan-lancaster unexpected mass incident self-mating system |
| CN109197578A (en) * | 2018-11-22 | 2019-01-15 | 山东省农业科学院玉米研究所（山东省农业科学院玉米工程技术研究中心） | A kind of selection of P crowds of corn inbred line Lx286 |
| CN109197578B (en) * | 2018-11-22 | 2021-11-05 | 山东省农业科学院玉米研究所（山东省农业科学院玉米工程技术研究中心） | Breeding method of P-group maize inbred line Lx286 |
| CN113647321A (en) * | 2021-07-20 | 2021-11-16 | 河北省农林科学院旱作农业研究所 | Efficient and rapid detection method for corn inbred line combining ability |
| CN113647321B (en) * | 2021-07-20 | 2022-05-27 | 河北省农林科学院旱作农业研究所 | Efficient and rapid detection method for corn inbred line combining ability |
| CN113475331A (en) * | 2021-08-05 | 2021-10-08 | 张掖市易农农业科技有限公司 | Planting method for corn DH line combining ability identification |
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Application publication date: 20160427 |