CN105145331A - Rice two-line sterile line breeding method - Google Patents
Rice two-line sterile line breeding method Download PDFInfo
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- CN105145331A CN105145331A CN201510394865.5A CN201510394865A CN105145331A CN 105145331 A CN105145331 A CN 105145331A CN 201510394865 A CN201510394865 A CN 201510394865A CN 105145331 A CN105145331 A CN 105145331A
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Abstract
The present invention discloses a rice two-line sterile line breeding method. According to the method, in the rice two-line sterile line later generation selection, the separate genetic characteristic exits in the sterility and the fertility of the early generation plant, the sterile plant selection is not performed in the early generation while the fertile plant is selected so as to perform rice quality analysis and other detections in the early generation, and the sterile plant selection is started to be performed in the 4th generation of hybrid so as to finally breed the rice two-line sterile line. According to the present invention, the sterile selection is not performed on the second generation of hybrid, and the fertile single-plant is selected so as to carry out the rice quality analysis in the early generation, such that the sterile line rice quality improving process is substantially accelerated, and the high-quality sterile line selection probability is improved; and the 3-4 generation of hybrid is subjected to main disease identification, such that the selection pressure of the sterile line is increased, it is ensured that the selected single plant has good disease resistance, the selected plant is reduced, and the manpower investment and the financial resource investment during the sterile line selection process are reduced.
Description
Technical field
The present invention relates to a kind of two-line sterile line of rice breeding method.
Background technology
In global atmosphere, carbon dioxide content rises the aggravation of the greenhouse effect caused, and global extreme weather gets more and more, and the natural calamity such as high temperature, arid occurs more and more frequently, increasing on the impact of Rice Production.At present, global integration process is more and more faster, Food Security has become the focus that the whole world is paid close attention to, it is overall not high that its Rice Production level of country mainly planted by current rice, improve the level of production of hybrid rice particularly two-line hybrid rice, whole world grain yield can be increased, ensure global peace and stable, China's science and technology influence power in the world can be improved simultaneously, the big country's image establishing China is had great significance.
Since the bright pine of stone found Nongken 58S in 1973, two-line hybrid rice development speed is more and more faster, due to advantages such as double-hybrid rice strains combo are free compared with Three-line Hybrid paddy rice, two-line sterile line causes breeder's pay attention to day by day, and obtains Nsfc Major Projects subsidy.Have increasing two-line sterile line to be applied in production at present, two-line hybrid rice is on the increase at national hybrid rice gross area proportion, for ensureing that the grain security of China has made huge contribution.
Current China two-line sterile line breeding method, follow and traditional select sterile strain positive season, Hainan adds the mode in generation, this selection also exists early for carrying out the shortcoming such as a meter quality detection, the breeding of every Dai Junxu transplanting of positive season regeneration strain, Breeding progress is slow, time-consuming takes a lot of work, therefore the present invention proposes a kind of new two-line sterile line breeding method, accelerates two-line sterile line and selects progress, reduce workload.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of two-line sterile line of rice breeding method.
In order to solve the problems of the technologies described above, the technical solution used in the present invention is, two-line sterile line of rice breeding method, comprises the following steps:
(1) use excellent two be restorer and two-line sterile line in combo, plantation F 1, harvest time, final election plant height was less than 1.2m, the combination that Comprehensive Traits is excellent, mixed receipts 1800 ~ 2200 seeds;
(2) the mixed F 1 seed received, during the plantation of hybrid 2 generation, plantation collective control is between 1000 ~ 1500 strains;
(3) when hybrid 2 generation, final election was selected in individual plant, plant type is selected according to two-line sterile line selection criteria, requires that tillering ability is strong, and plant height is between 80cm ~ 85cm, and sword-like leave is endured and curls inward slightly, and ripening rate is more than 85%; Plant type is selected can educate individual plant, gathers in the crops whole seed, carries out a meter matter analysis in indoor, eliminates exterior quality and the poor individual plant of processing quality;
(4) in selected hybrid 2 generation, the plantation of per stirpes plant forms hybrid 3 generation, presses different times Inoculated Rice Major Diseases in whole growing process, as bacterial leaf spot, rice blast etc., the time of infertility investigates disease resistance, and final election disease resistance is good, the educated individual plant that plant type is excellent;
(5) selected hybrid 3 generation individual plant, forms hybrid 4 generation selection colony by strain plantation; Hybrid 4 generation and hybrid 3 generation the same manner carry out disease resistance selection, carry out microscopy Pollen Activity simultaneously, eliminate male sterile line difference strain;
(6) two-line sterile line cultivate 5 generation colony start to carry out combining ability test, measure carry out final election in conjunction with field diseases qualification, the analysis of indoor rice matter, origin temp simultaneously, the final two-line sterile line of rice combining better resistance, rice is of fine quality, general combining ability is better, origin temp 23 DEG C ~ 24 DEG C, sterile rate are 100% of selecting is identified.
The invention has the beneficial effects as follows:
1) in hybrid 2 generation, does not carry out sterility selection, and selection can be educated individual plant and early for carrying out a meter matter analysis, greatly can be accelerated the process that male sterile line rice matter promotes, increasing the probability that good quality male sterile line is selected.
2) in hybrid 3 ~ 4 generation, carries out Major Diseases qualification, increases the Selective Pressure of male sterile line, ensures that selected individual plant has good disease resistance, decreases selected individual plant, decrease the input of manpower and financial resources in male sterile line selection course.
3) only after separation 4 generation, select sterile strain in whole rice sterile line selection course, decrease the transplanting number of male sterile line regeneration strain, increase the combo number of selection, add the probability cultivating excellent two-line sterile line.
4) the method is early selected for not carrying out sterile selecting good strains in the field for seed, and does not limit by breeding place, can reduce two-line sterile line of rice and select difficulty, greatly reduce field work amount.
Embodiment
(1) use excellent two be restorer and two-line sterile line in combo, plantation F 1, harvest time, final election plant height was less than 1.2m, the combination that Comprehensive Traits is excellent, mixed receipts about 2000 seeds;
(2) the mixed F 1 seed received, during the plantation of hybrid 2 generation, plantation collective control is between 1000 ~ 1500 strains;
(3) when hybrid 2 generation, final election was selected in individual plant, plant type is selected according to two-line sterile line selection criteria, requires that tillering ability is strong, and plant height is between 80cm ~ 85cm, and sword-like leave is endured and curls inward slightly, and ripening rate is more than 85%; Plant type is selected can educate individual plant, gathers in the crops whole seed, carries out a meter matter analysis in indoor, eliminates exterior quality and the poor individual plant of processing quality;
(4) in selected hybrid 2 generation, the plantation of per stirpes plant forms hybrid 3 generation, presses different times Inoculated Rice Major Diseases in whole growing process, as bacterial leaf spot, rice blast etc., the time of infertility investigates disease resistance, and final election disease resistance is good, the educated individual plant that plant type is excellent;
(5) selected hybrid 3 generation individual plant, forms hybrid 4 generation selection colony by strain plantation; Hybrid 4 generation and hybrid 3 generation the same manner carry out disease resistance selection, carry out microscopy Pollen Activity simultaneously, eliminate male sterile line difference strain;
(6) two-line sterile line cultivate 5 generation colony start to carry out combining ability test, measure carry out final election in conjunction with field diseases qualification, the analysis of indoor rice matter, origin temp simultaneously, final select to combine better resistance, rice is of fine quality, general combining ability is better, two-line sterile line of rice that origin temp about 23.5 DEG C, sterile rate are the feature such as 100% identifies.
Above-described embodiment of the present invention, does not form limiting the scope of the present invention.Any amendment done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within claims of the present invention.
Claims (1)
1. two-line sterile line of rice breeding method, comprises the following steps:
(1) use excellent two be restorer and two-line sterile line in combo, plantation F 1, harvest time, final election plant height was less than 1.2m, the combination that Comprehensive Traits is excellent, mixed receipts 1800 ~ 2200 seeds;
(2) the mixed F 1 seed received, during the plantation of hybrid 2 generation, plantation collective control is between 1000 ~ 1500 strains;
(3) when hybrid 2 generation, final election was selected in individual plant, plant type is selected according to two-line sterile line selection criteria, requires that tillering ability is strong, and plant height is between 80cm ~ 85cm, and sword-like leave is endured and curls inward slightly, and ripening rate is more than 85%; Plant type is selected can educate individual plant, gathers in the crops whole seed, carries out a meter matter analysis in indoor, eliminates exterior quality and the poor individual plant of processing quality;
(4) in selected hybrid 2 generation, the plantation of per stirpes plant forms hybrid 3 generation, presses different times Inoculated Rice Major Diseases in whole growing process, as bacterial leaf spot, rice blast etc., the time of infertility investigates disease resistance, and final election disease resistance is good, the educated individual plant that plant type is excellent;
(5) selected hybrid 3 generation individual plant, forms hybrid 4 generation selection colony by strain plantation; Hybrid 4 generation and hybrid 3 generation the same manner carry out disease resistance selection, carry out microscopy Pollen Activity simultaneously, eliminate male sterile line difference strain;
(6) two-line sterile line cultivate 5 generation colony start to carry out combining ability test, measure carry out final election in conjunction with field diseases qualification, the analysis of indoor rice matter, origin temp simultaneously, the final two-line sterile line of rice combining better resistance, rice is of fine quality, general combining ability is better, origin temp 23 DEG C ~ 24 DEG C, sterile rate are 100% of selecting is identified.
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| CN201510394865.5A CN105145331A (en) | 2015-07-07 | 2015-07-07 | Rice two-line sterile line breeding method |
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| CN201510394865.5A CN105145331A (en) | 2015-07-07 | 2015-07-07 | Rice two-line sterile line breeding method |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101919351A (en) * | 2009-06-11 | 2010-12-22 | 北京金色农华种业科技有限公司 | Production method of hybrid rice |
| CN103518616A (en) * | 2013-10-14 | 2014-01-22 | 广西恒茂农业科技有限公司 | Method for breeding rice hybrid Y Liangyou 087 |
| CN104542247A (en) * | 2014-12-15 | 2015-04-29 | 广西大学 | Breeding method of dual-purpose genic male sterile line of rice |
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- 2015-07-07 CN CN201510394865.5A patent/CN105145331A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101919351A (en) * | 2009-06-11 | 2010-12-22 | 北京金色农华种业科技有限公司 | Production method of hybrid rice |
| CN103518616A (en) * | 2013-10-14 | 2014-01-22 | 广西恒茂农业科技有限公司 | Method for breeding rice hybrid Y Liangyou 087 |
| CN104542247A (en) * | 2014-12-15 | 2015-04-29 | 广西大学 | Breeding method of dual-purpose genic male sterile line of rice |
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