CN101218891A - Transfer method for non glutinous rice infertility series with high column dead exposing ratio - Google Patents
Transfer method for non glutinous rice infertility series with high column dead exposing ratio Download PDFInfo
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- CN101218891A CN101218891A CNA200810052242XA CN200810052242A CN101218891A CN 101218891 A CN101218891 A CN 101218891A CN A200810052242X A CNA200810052242X A CN A200810052242XA CN 200810052242 A CN200810052242 A CN 200810052242A CN 101218891 A CN101218891 A CN 101218891A
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- 240000007594 Oryza sativa Species 0.000 title claims description 19
- 235000007164 Oryza sativa Nutrition 0.000 title claims description 19
- 235000009566 rice Nutrition 0.000 title claims description 19
- 238000000034 method Methods 0.000 title claims description 10
- 230000036512 infertility Effects 0.000 title claims description 9
- 208000000509 infertility Diseases 0.000 title 1
- 231100000535 infertility Toxicity 0.000 title 1
- 240000008467 Oryza sativa Japonica Group Species 0.000 claims abstract description 68
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 50
- 238000012423 maintenance Methods 0.000 claims abstract description 47
- 241000196324 Embryophyta Species 0.000 claims abstract description 32
- 239000000463 material Substances 0.000 claims abstract description 32
- 230000000306 recurrent effect Effects 0.000 claims abstract description 6
- 238000009396 hybridization Methods 0.000 claims description 19
- 230000009286 beneficial effect Effects 0.000 claims description 8
- 240000002853 Nelumbo nucifera Species 0.000 claims description 5
- 235000006508 Nelumbo nucifera Nutrition 0.000 claims description 5
- 235000006510 Nelumbo pentapetala Nutrition 0.000 claims description 5
- 230000002146 bilateral effect Effects 0.000 claims description 5
- 235000013339 cereals Nutrition 0.000 claims description 5
- 230000035558 fertility Effects 0.000 claims description 5
- 239000010903 husk Substances 0.000 claims description 5
- 230000006798 recombination Effects 0.000 claims description 5
- 238000005204 segregation Methods 0.000 claims description 4
- 241000746966 Zizania Species 0.000 claims description 3
- 235000002636 Zizania aquatica Nutrition 0.000 claims description 3
- 230000001195 anabolic effect Effects 0.000 claims description 3
- 238000006116 polymerization reaction Methods 0.000 claims description 3
- 230000004075 alteration Effects 0.000 abstract 2
- 238000009395 breeding Methods 0.000 abstract 1
- 244000184734 Pyrus japonica Species 0.000 description 4
- 230000009466 transformation Effects 0.000 description 3
- 230000007812 deficiency Effects 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 238000012214 genetic breeding Methods 0.000 description 1
- 238000009405 line breeding Methods 0.000 description 1
- 230000005070 ripening Effects 0.000 description 1
Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
Abstract
A breeding method for a sterile line of the japonica rice with a high stigma exsertion rate relates to:(1) selecting the materials with big stigmas or high stigma exsertion rate; (2) hybridizing a donor parent carrying the genes of the stigma exsertion characters with a parent material of the japonica rice; (3) selecting the single plant with a stigma exsertion rate more than 95 percent to acquire a maintenance line with a stigma exsertion rate more than 95 percent beginning from the F2 generation of a hybrid segregative generation;(4) using the maintenance line obtained from step (3) to hybridize with the sterile line of the japonica rice carrying sterile genes and using the maintenance line as a recurrent parent to carry out continuous backcross to obtain a sterile line of the japonica rice with a high stigma exsertion rate and basically stable characters. The invention has the advantages of that: 1. the invention is suitable for maintaining the existing sterile line, carrying out alteration on the stigma exsertion rate and improving the stigma exsertion rate of the existing sterile line of the japonica rice; 2. the invention can directionally realize the alteration on the improvement of the stigma exsertion rate on all materials including the maintenance line of the existing sterile line of the japonica rice with a lower stigma exsertion rate, thus further improving the yield of seed production.
Description
(1) technical field:
The invention belongs to Japonica Hybrid genetic breeding and production of hybrid seeds field, especially a kind of selection of high stigma exposure rate japonica rice rice male sterile line.
(2) background technology:
The Asia cultivated rice has two subspecies of Xian round-grained rice, and typical indica type paddy rice majority has the proterties of stigma appearing, and the Typical Japonica stigma exposing ratio is extremely low, and general about 0.2%.So far, mostly japonica rice sterile is to have kept the low characteristic of japonica rice stigma exposing ratio by conventional japonica rice kind or strain transformation.Because Japonica Hybrid is recovered gene from long-grained nonglutinous rice, recover system during the production of hybrid seeds and bloom early, male sterile line is bloomed late, cause blooming time overslip, because the paddy rice floret opening time is shorter, make male sterile line can not accept the pollen fertilization again, final hybrid seed yield is low, becomes the bottleneck of Japonica Hybrid production development.Because blooming time overslip, the male sterile line column cap does not expose, even if flower synchronization, general production of hybrid seeds ripening rate is all below 30%.
(3) summary of the invention:
Goal of the invention of the present invention is to invent a kind of selection of high stigma exposure rate japonica rice rice male sterile line, and it can overcome deficiency of the prior art, obtains high stigma exposure rate japonica rice rice male sterile line, and uses it for the production of hybrid seeds production of advantage combination.
Technical scheme of the present invention is: a kind of selection of high stigma exposure rate japonica rice rice male sterile line is characterized in that it is made of following steps:
(1) selects big column cap and the high material of stigma exposing ratio, as the donor parents of stigma appearing character gene;
(2) hybridization of the donor parents of stigma appearing character gene and japonica rice parent material be will carry, beneficial gene and sterility maintenance gene pyramiding and the high genetic recombination of stigma exposing ratio made;
(3) from hybridization segregative generation F2 for, the selection stigma exposing ratio reaches the individual plant more than 95%, by selfing, continue to select the strain system of stigma exposing ratio more than 95%, by hybridizing with male sterile line, measure the hold facility that it has sterile gene, obtain the maintenance line of stigma exposing ratio more than 95% thus;
(4) with maintenance line that obtains in the step (3) and the japonica rice sterile hybridization of carrying sterile gene, make the recurrent parent continuous backcross with this maintenance line, backcross 4-5 time, the high stigma exposure rate japonica rice rice male sterile line that acquired character is basicly stable, this high stigma exposure rate japonica rice rice male sterile line is except that fertility, and other economical character is identical with the maintenance line parent.
The material that big column cap in the above-mentioned said step (1) and stigma exposing ratio are high is big and high long-grained nonglutinous rice, java rice or the wild rice material of stigma exposing ratio of column cap.
Japonica rice parent material in the above-mentioned said step (2) is the japonica rice maintenance line, or has the sterile gene hold facility, and has the japonica rice material of advantage on output, quality, resistance, plant type economical character.
The method that obtains the maintenance line of stigma exposing ratio more than 95% in the above-mentioned said step (3) has two kinds: one is the individual plant that at first selection has the stigma appearing proterties in the hybridization segregation population, requiring stigma exposing ratio to reach in the individual plant more than 90% selects, in the individual plant that possesses the stigma appearing proterties, select plant type and the outstanding individual plant of economical character then, thereby obtain engineered maintenance line; Two is that the individual plant that stigma exposing ratio reaches more than 95% is continued to reestablish diplomatic relations or backcross with the parent with high-quality, high yield, high resistance shape, with the stigma exposing ratio is the preferential proterties of selecting, make beneficial gene and sterility keep gene and the polymerization of stigma appearing character gene, obtain made maintenance line.
Sterile gene in the above-mentioned said step (4) comprises BT type, Yunnan one type, red lotus type or wild type.
The high stigma exposure rate japonica rice rice male sterile line that obtains in the above-mentioned said step (4) can be directly used in the production of hybrid seeds production of advantage japonica rice combination, also can newly make up to survey and join seed selection work in the new anabolic process of seed selection.
After above-mentioned said stigma exposing ratio was meant that the paddy rice floret opening closes grain husk, column cap was given prominence to the clever exposing ratio that closes outside closed Ying's shell, comprised two kinds of situations of bilateral exposing ratio and monolateral exposing ratio.
Superiority of the present invention is: 1. the present invention is applicable to the stigma exposing ratio transformation is carried out in the maintenance of existing male sterile line, improves the stigma exposing ratio of existing japonica rice sterile; 2. to all japonica rice materials, comprise the maintenance line of the japonica rice sterile that present stigma exposing ratio is low, can both orientation realize the transformation that stigma exposing ratio improves, and then improve hybrid seed yield.
(4) embodiment:
Embodiment 1: a kind of selection of high stigma exposure rate japonica rice rice male sterile line is characterized in that it is made of following steps:
(1) selects big column cap and the high material of stigma exposing ratio, as the donor parents of stigma appearing character gene;
(2) hybridization of the donor parents of stigma appearing character gene and japonica rice parent material be will carry, beneficial gene and sterility maintenance gene pyramiding and the high genetic recombination of stigma exposing ratio made;
(3) from hybridization segregative generation F2 for, the selection stigma exposing ratio reaches the individual plant more than 95%, by selfing, continue to select the strain system of stigma exposing ratio more than 95%, by hybridizing with male sterile line, measure the hold facility that it has sterile gene, obtain the maintenance line of stigma exposing ratio more than 95% thus;
(4) with maintenance line that obtains in the step (3) and the japonica rice sterile hybridization of carrying sterile gene, make the recurrent parent continuous backcross with this maintenance line, backcross 4-5 time, the high stigma exposure rate japonica rice rice male sterile line that acquired character is basicly stable, this high stigma exposure rate japonica rice rice male sterile line is except that fertility, and other economical character is identical with the maintenance line parent.
The material that big column cap in the above-mentioned said step (1) and stigma exposing ratio are high is the big and high long-grained nonglutinous rice material of stigma exposing ratio of column cap.
Japonica rice parent material in the above-mentioned said step (2) is the japonica rice maintenance line.
The method that obtains the maintenance line of stigma exposing ratio 95% or more in the above-mentioned said step (3) is: the individual plant that selection has the stigma appearing proterties in the hybridization segregation population at first, requiring stigma exposing ratio to reach in the individual plant more than 90% selects, in the individual plant that possesses the stigma appearing proterties, select plant type and the outstanding individual plant of economical character then, thereby obtain engineered maintenance line.
Sterile gene in the above-mentioned said step (4) comprises BT type, Yunnan one type, red lotus type or wild type.
The high stigma exposure rate japonica rice rice male sterile line that obtains in the above-mentioned said step (4) is directly used in the production of hybrid seeds production of advantage japonica rice combination.
After above-mentioned said stigma exposing ratio was meant that the paddy rice floret opening closes grain husk, column cap was given prominence to the clever exposing ratio that closes outside closed Ying's shell, comprised two kinds of situations of bilateral exposing ratio and monolateral exposing ratio.
Embodiment 2: a kind of selection of high stigma exposure rate japonica rice rice male sterile line is characterized in that it is made of following steps:
(1) selects big column cap and the high material of stigma exposing ratio, as the donor parents of stigma appearing character gene;
(2) hybridization of the donor parents of stigma appearing character gene and japonica rice parent material be will carry, beneficial gene and sterility maintenance gene pyramiding and the high genetic recombination of stigma exposing ratio made;
(3) from hybridization segregative generation F2 for, the selection stigma exposing ratio reaches the individual plant more than 95%, by selfing, continue to select the strain system of stigma exposing ratio more than 95%, by hybridizing with male sterile line, measure the hold facility that it has sterile gene, obtain the maintenance line of stigma exposing ratio more than 95% thus;
(4) with maintenance line that obtains in the step (3) and the japonica rice sterile hybridization of carrying sterile gene, make the recurrent parent continuous backcross with this maintenance line, backcross 4-5 time, the high stigma exposure rate japonica rice rice male sterile line that acquired character is basicly stable, this high stigma exposure rate japonica rice rice male sterile line is except that fertility, and other economical character is identical with the maintenance line parent.
The material that big column cap in the above-mentioned said step (1) and stigma exposing ratio are high is the big and high long-grained nonglutinous rice material of stigma exposing ratio of column cap.
Japonica rice parent material in the above-mentioned said step (2) is the japonica rice material that has the sterile gene hold facility and have advantage on output, quality, resistance, plant type economical character.
The method that obtains the maintenance line of stigma exposing ratio more than 95% in the above-mentioned said step (3) is: the individual plant that stigma exposing ratio is reached more than 95% continues to reestablish diplomatic relations or backcross with the parent with high-quality, high yield, high resistance shape, with the stigma exposing ratio is the preferential proterties of selecting, make beneficial gene and sterility keep gene and the polymerization of stigma appearing character gene, obtain made maintenance line.
Sterile gene in the above-mentioned said step (4) comprises BT type, Yunnan one type, red lotus type or wild type.
The high stigma exposure rate japonica rice rice male sterile line that obtains in the above-mentioned said step (4) newly makes up to survey and joins seed selection work in the new anabolic process of seed selection.
After above-mentioned said stigma exposing ratio was meant that the paddy rice floret opening closes grain husk, column cap was given prominence to the clever exposing ratio that closes outside closed Ying's shell, comprised two kinds of situations of bilateral exposing ratio and monolateral exposing ratio.
Embodiment 3: a kind of selection of high stigma exposure rate japonica rice rice male sterile line is characterized in that it is made of following steps:
(1) selects big column cap and the high material of stigma exposing ratio, as the donor parents of stigma appearing character gene;
(2) hybridization of the donor parents of stigma appearing character gene and japonica rice parent material be will carry, beneficial gene and sterility maintenance gene pyramiding and the high genetic recombination of stigma exposing ratio made;
(3) from hybridization segregative generation F2 for, the selection stigma exposing ratio reaches the individual plant more than 95%, by selfing, continue to select the strain system of stigma exposing ratio more than 95%, by hybridizing with male sterile line, measure the hold facility that it has sterile gene, obtain the maintenance line of stigma exposing ratio more than 95% thus;
(4) with maintenance line that obtains in the step (3) and the japonica rice sterile hybridization of carrying sterile gene, make the recurrent parent continuous backcross with this maintenance line, backcross 4-5 time, the high stigma exposure rate japonica rice rice male sterile line that acquired character is basicly stable, this high stigma exposure rate japonica rice rice male sterile line is except that fertility, and other economical character is identical with the maintenance line parent.
The material that big column cap in the above-mentioned said step (1) and stigma exposing ratio are high is big and high long-grained nonglutinous rice, java rice and the wild rice material of stigma exposing ratio of column cap.
Japonica rice parent material in the above-mentioned said step (2) is the japonica rice maintenance line.
The method that obtains the maintenance line of stigma exposing ratio 95% or more in the above-mentioned said step (3) is: the individual plant that selection has the stigma appearing proterties in the hybridization segregation population at first, requiring stigma exposing ratio to reach in the individual plant more than 90% selects, in the individual plant that possesses the stigma appearing proterties, select plant type and the outstanding individual plant of economical character then, thereby obtain engineered maintenance line.
Sterile gene in the above-mentioned said step (4) comprises BT type, Yunnan one type, red lotus type or wild type.
The high stigma exposure rate japonica rice rice male sterile line that obtains in the above-mentioned said step (4) is directly used in the production of hybrid seeds production of advantage japonica rice combination.
After above-mentioned said stigma exposing ratio was meant that the paddy rice floret opening closes grain husk, column cap was given prominence to the clever exposing ratio that closes outside closed Ying's shell, comprised two kinds of situations of bilateral exposing ratio and monolateral exposing ratio.
Claims (7)
1. the selection of a high stigma exposure rate japonica rice rice male sterile line is characterized in that it is made of following steps:
(1) selects big column cap and the high material of stigma exposing ratio, as the donor parents of stigma appearing character gene;
(2) hybridization of the donor parents of stigma appearing character gene and japonica rice parent material be will carry, beneficial gene and sterility maintenance gene pyramiding and the high genetic recombination of stigma exposing ratio made;
(3) from hybridization segregative generation F2 for, the selection stigma exposing ratio reaches the individual plant more than 95%, by selfing, continue to select the strain system of stigma exposing ratio more than 95%, by hybridizing with male sterile line, measure the hold facility that it has sterile gene, obtain the maintenance line of stigma exposing ratio more than 95% thus;
(4) with maintenance line that obtains in the step (3) and the japonica rice sterile hybridization of carrying sterile gene, make the recurrent parent continuous backcross with this maintenance line, backcross 4-5 time, the high stigma exposure rate japonica rice rice male sterile line that acquired character is basicly stable, this high stigma exposure rate japonica rice rice male sterile line is except that fertility, and other economical character is identical with the maintenance line parent.
2. according to the selection of the said a kind of high stigma exposure rate japonica rice rice male sterile line of claim 1, it is characterized in that big column cap and the high material of stigma exposing ratio in the said step (1) is big and high long-grained nonglutinous rice, java rice or the wild rice material of stigma exposing ratio of column cap.
3. according to the selection of the said a kind of high stigma exposure rate japonica rice rice male sterile line of claim 1, it is characterized in that the japonica rice parent material in the said step (2) is the japonica rice maintenance line, or have sterile gene hold facility and a japonica rice material that on output, quality, resistance, plant type economical character, has advantage.
4. according to the selection of the said a kind of high stigma exposure rate japonica rice rice male sterile line of claim 1, it is characterized in that the method that obtains the maintenance line of stigma exposing ratio more than 95% in the said step (3) has two kinds: one is the individual plant that at first selection has the stigma appearing proterties in the hybridization segregation population, requiring stigma exposing ratio to reach in the individual plant more than 90% selects, in the individual plant that possesses the stigma appearing proterties, select plant type and the outstanding individual plant of economical character then, thereby obtain engineered maintenance line; Two is that the individual plant that stigma exposing ratio reaches more than 95% is continued to reestablish diplomatic relations or backcross with the parent with high-quality, high yield, high resistance shape, with the stigma exposing ratio is the preferential proterties of selecting, make beneficial gene and sterility keep gene and the polymerization of stigma appearing character gene, obtain made maintenance line.
5. according to the selection of the said a kind of high stigma exposure rate japonica rice rice male sterile line of claim 1, it is characterized in that the sterile gene in the said step (4) comprises BT type, Yunnan one type, red lotus type or wild type.
6. according to the selection of the said a kind of high stigma exposure rate japonica rice rice male sterile line of claim 1, it is characterized in that the high stigma exposure rate japonica rice rice male sterile line that obtains in the said step (4) can be directly used in the production of hybrid seeds production of advantage japonica rice combination, also can in the new anabolic process of seed selection, newly make up to survey and join seed selection work.
7. according to the selection of claim 1,2,4 or 6 said a kind of high stigma exposure rate japonica rice rice male sterile lines, after it is characterized in that said stigma exposing ratio is meant that the paddy rice floret opening closes grain husk, column cap is given prominence to the clever exposing ratio that closes outside closed Ying's shell, comprises two kinds of situations of bilateral exposing ratio and monolateral exposing ratio.
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| CN200810052242XA CN101218891B (en) | 2008-02-02 | 2008-02-02 | Transfer method for non glutinous rice infertility series with high column dead exposing ratio |
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| CN200810052242XA CN101218891B (en) | 2008-02-02 | 2008-02-02 | Transfer method for non glutinous rice infertility series with high column dead exposing ratio |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103609430A (en) * | 2013-11-19 | 2014-03-05 | 浙江省农业科学院 | Method for selecting and reproducing early-flowering late japonica rice sterile line Zhe japonica rice 4A with high stigma exposure rate |
| CN104170716A (en) * | 2013-12-19 | 2014-12-03 | 天津天隆种业科技有限公司 | Seed selection method of high stigma exsertion rate japonica two-line sterile line |
| CN104186302A (en) * | 2013-12-19 | 2014-12-10 | 国家粳稻工程技术研究中心 | Technical method for multi-genotype heterosis utilization by utilizing photoperiod non-sensitive genetic male-sterile line |
| CN105494086A (en) * | 2016-01-20 | 2016-04-20 | 安徽理想种业有限公司 | Breeding method for sterile line with high outcrossing rate of two-line japonica rice |
| CN106350583A (en) * | 2016-08-26 | 2017-01-25 | 中国农业科学院棉花研究所 | Molecular identification method of CGSS (cotton germplasm with stigma showing) gene |
| CN108901827A (en) * | 2018-07-13 | 2018-11-30 | 重庆市农业科学院 | Japonica hybrid rice sterile line breeding method when a kind of early blossoming |
| CN112586338A (en) * | 2020-12-04 | 2021-04-02 | 国家粳稻工程技术研究中心 | Breeding method of japonica rice sterile line in early flowering |
| CN114902955A (en) * | 2022-06-08 | 2022-08-16 | 吉林省农业科学院 | Method for breeding mung bean stigma-exposed male sterile line |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1843087A (en) * | 2005-12-27 | 2006-10-11 | 辽宁省稻作研究所 | High stigma exposure rate japonica rice sterile line selection and breeding technology |
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2008
- 2008-02-02 CN CN200810052242XA patent/CN101218891B/en active Active
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103609430A (en) * | 2013-11-19 | 2014-03-05 | 浙江省农业科学院 | Method for selecting and reproducing early-flowering late japonica rice sterile line Zhe japonica rice 4A with high stigma exposure rate |
| CN103609430B (en) * | 2013-11-19 | 2015-04-29 | 浙江省农业科学院 | Method for selecting and reproducing early-flowering late japonica rice sterile line Zhe japonica rice 4A with high stigma exposure rate |
| CN104170716A (en) * | 2013-12-19 | 2014-12-03 | 天津天隆种业科技有限公司 | Seed selection method of high stigma exsertion rate japonica two-line sterile line |
| CN104186302A (en) * | 2013-12-19 | 2014-12-10 | 国家粳稻工程技术研究中心 | Technical method for multi-genotype heterosis utilization by utilizing photoperiod non-sensitive genetic male-sterile line |
| CN104170716B (en) * | 2013-12-19 | 2016-09-14 | 天津天隆种业科技有限公司 | A kind of selection of high stigma exposure rate japonica rice type two-line sterile line |
| CN105494086A (en) * | 2016-01-20 | 2016-04-20 | 安徽理想种业有限公司 | Breeding method for sterile line with high outcrossing rate of two-line japonica rice |
| CN106350583A (en) * | 2016-08-26 | 2017-01-25 | 中国农业科学院棉花研究所 | Molecular identification method of CGSS (cotton germplasm with stigma showing) gene |
| CN106350583B (en) * | 2016-08-26 | 2019-09-10 | 中国农业科学院棉花研究所 | A kind of method for identifying molecules of cotton stigma appearing gene |
| CN108901827A (en) * | 2018-07-13 | 2018-11-30 | 重庆市农业科学院 | Japonica hybrid rice sterile line breeding method when a kind of early blossoming |
| CN112586338A (en) * | 2020-12-04 | 2021-04-02 | 国家粳稻工程技术研究中心 | Breeding method of japonica rice sterile line in early flowering |
| CN114902955A (en) * | 2022-06-08 | 2022-08-16 | 吉林省农业科学院 | Method for breeding mung bean stigma-exposed male sterile line |
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| CN101218891B (en) | 2011-07-13 |
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Denomination of invention: Transfer method for non glutinous rice infertility series with high column dead exposing ratio Effective date of registration: 20130711 Granted publication date: 20110713 Pledgee: Tianjin TEDA Small Enterprise Credit Guarantee Center Pledgor: Tianjin Tianlong Seeds Science and Technology Co., Ltd. Registration number: 2013120000017 |
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