CN105409758B - A kind of locust tree artificial emasculation and hybridization controlled pollination method - Google Patents
A kind of locust tree artificial emasculation and hybridization controlled pollination method Download PDFInfo
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- CN105409758B CN105409758B CN201510783041.7A CN201510783041A CN105409758B CN 105409758 B CN105409758 B CN 105409758B CN 201510783041 A CN201510783041 A CN 201510783041A CN 105409758 B CN105409758 B CN 105409758B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
- A01H1/027—Apparatus for pollination
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Abstract
The invention discloses a kind of locust tree artificial emasculation and hybridization controlled pollination method, comprise the following steps：（1）Make pollination device；（2）Selected parent sire of hybrid pigs and maternal plant,（3）Harvesting pollen,（4）Artificial pollination,（5）Pollination maternal plant management,（6）The ripe pod harvesting of hybridization；This method is described in detail to the technical step of Pagoda Flower emasculation, pollination, and petal and gynoecium are not damaged, can significantly improve locust tree artificial pollination setting percentage；Improve locust tree artificial hybridization success rate of breeding.
Description
Technical field
The invention belongs to field of plant variety breeding technology, and in particular to a kind of locust tree artificial emasculation and hybridization controlled pollination side
Method.
Background technology
Locust tree (Robinia pseudoacacia L.) is pulse family Papillionoideae robinia broad-leaved megaphanerophyte, originates in U.S.
(1601) are introduced into Europe at the beginning of state, the 17th century, and European Countries are spread all over to 18th century.19 end of the centurys are by German first by locust tree
Introduces a collection introduces Chinese Qingdao, has 40~sixties of 20th century several locust tree introduces a collections from Japan, the U.S., Korea to be planted in me again
The ground such as the state east area of the Liao River, coextensive with eastern and southern Liaoning Province, Tianshui city, Changsha and North China, to late 1980s, China has 24 provinces, municipalities and autonomous regions to plant
Plant locust tree, the only province of Hebei, Henan, Shandong, Shanxi, Liaoning etc. 6 just up to 4,000,000,000 plants, as China North China, northwest, THE SOUTH OF NORTHEAST CHINA,
The main Timber stands of all provinces of Yangtze-Huaihe River Valley, firewood forests, forests for water and soil conservation, sea wall and river levee shelter-forest seeds.Locust tree is thick with leaves,
The flowers are in blossom, and fragrance is strong, is high-quality nectar source seeds；Locust tree timber is tough and tensile, shock resistance, resistance to rotten, water-fast wet, is important building, ore deposit
Post, vehicle, shipbuilding material；Locust tree timber calorific value is high, and burning velocity is slow, is high-quality fire wood and excellent Tree Species as Bio-energy；
Locust tree greenery nutrient composition content is high, nonpoisonous and tasteless, is first-class animal feeding-stuff.Locust tree is used as many value, multipurpose, resistance
The strong good seeds of good, suitable raw ability, the application prospect of its improved Varieties is very wide.
Domestic and international locust tree breeding for a long time is main based on selection and use technology, few to use artificial hybridization breeding skill
Art.Selection and use technology is to find, select and utilize natural excellent hereditary variation individual plant or type, and method is simple and easy to apply, cost
It is relatively low, but breeding objective can not be determined according to artificial wish, can not independently select to determine hybrid strain.Artificial hybridization breeding
Technology can be selected to be adapted to the hybridization Parent of breeding objective according to artificial wish, and artificialpollination process is obtained super close miscellaneous
Jiao zi generation.By the measure to hybrid generation, screening and utilization, improved Varieties are obtained.
Locust tree is the same flowering plant of monoecism, hermaphrodite flower.Raceme, the crown of papilionaceous flower, 5 pieces of petal is divided into 1 piece of vexil, 2
Piece ala, 2 pieces of fossil fragments, as shown in Figure 4.Order of blooming is that vexil is first opened a business, and 2 alas are opened a business afterwards, and 2 fossil fragments will
Gynoecium and stamen inclusion are in together sickle, until vexil and the withered decline of ala are not also opened a business.So, Pagoda Flower open when from
Outward appearance can't see flower pesticide and column cap, and need to push petal aside by instrument can just expose stamen and gynoecium, and this is manually awarded to crossbreeding
Powder brings great difficulty.Artificial pollination should remove stamen, also to push petal aside and expose column cap, and this brings damage, shadow to flower
Its normal physiological and biochemical procedure is rung, artificial pollination setting percentage is less than Natural seed setting rate.Locust tree Natural seed setting rate is relatively low, averagely
30%, this causes that locust tree artificial hybridization success rate of breeding is restricted.Our result of the test is, using conventional method to locust tree
Its setting percentage of artificialpollination 1%~5%, document in addition《Locust tree blooms and pollinates and mating pattern》Northeast Forestry University is learned
Report, it is only 3.56% that open artificial emasculation hybridizes average fruit-setting rate；The relevant locust tree artificial pollination setting percentage of hitherto reported also exists
In the range of this.
The content of the invention
For the problem that current crossbreeding technology is present, the present invention provides a kind of to Pagoda Flower emasculation, pollination, without damaging
Hinder the method for petal and gynoecium, and invent a kind of pollination device.Manually control is awarded can to complete locust tree using the method and pollination device
Powder process, obtains hybrid seed.
A kind of locust tree artificial emasculation and hybridization controlled pollination method, comprise the following steps：
(1) pollination device is made：The pollination device includes containing polliniferous vial, and vial is closed using bottle closure of rubber,
Bottle closure of rubber in the vial is connected by wire with rubber；
(2) parent sire of hybrid pigs and maternal plant are selected；
(3) pollen is harvested；
(4) emasculation, pollination：In maternal plant selection pollination inflorescence, inflorescence is held, pinch flower, the vexil of flower is dialled
Open, expose stamen, pinch filigree upper end and remove flower pesticide；Style and column cap are stirred after emasculation, allows column cap to expose from petal, so
Afterwards using the pollination device alignment column cap pollination of adhesion pollen, style and column cap are sent back in petal with tweezers afterwards, allow pollination
Style and column cap recover physical slot；
(5) pollination maternal plant management；
(6) ripe pod harvesting is hybridized.
In step (1), the present invention designs a kind of pollination device of suitable Pagoda Flower, bag according to the growth characteristic of locust tree inflorescence
Include the cylindrical type rubber contained polliniferous vial and wire is connected for a head sharpening other end.
The preparation method of the pollination device is that rubber is cut into 1.8~2.2cm (preferably 2cm) long, 0.8~1.2cm of diameter
The cylinder of (preferably 1cm), will wherein one sharpening, for gluing pollen, other end is scabbled, centre insertion a piece metal
Silk, is connected, 5~10cm of thin wire length (preferably 10cm) with the bottle closure of rubber in vial.
Wherein vial highly 4~6cm (preferably 5cm), 1.5~2.5cm of diameter (preferably 2cm), cylindrical rubber bottle stopper.
The pollination device is that applicant gropes to it is best suitable for being used when Pagoda Flower is pollinated, its work by long-term practice
Make efficiency and pollination success rate is higher.
The use writing brush having in the prior art is used as pollination device, but locust tree is smeared the effect of pollen and pays no attention to using writing brush
Think, the property of physilogical characteristics and locust tree pollen according to Pagoda Flower, the present invention uses above-mentioned rubber pollination device, easy, easy to operate,
By substantial amounts of verification experimental verification, gynoecium pollination success rate and efficiency high do not damage petal and gynoecium.
Making pollination device from rubber has four advantages, and one is that rubber quality is soft, flexible and toughness, it is easy to which iron wire is inserted
And it is fixed；Two is to be easy to pollen of adhering；Three is to make easy, low cost；Four are combined to be made and are awarded with the bottle closure of rubber in vial
Powder device, pollen contamination rate is small, and pollination efficiency is improved.
In step (2), the method for selected parent sire of hybrid pigs and maternal plant is：The locust tree that selection enters generative growth phase is excellent
6~20 years raw healthy and strong trees of non-defective unit kind Middle and young forest are used as hybridization Parent plant.
The healthy and strong strain of 6~20 years growing ways of the selection age of tree as hybridization Parent plant, less than 6 years locust trees of the age of tree due to
Its own should not implement artificial pollination not yet completely into generative growth phase；The strain raw more than 20 years is due to the life of itself
Long and nutritive peculiarity, the problems such as cause organ of multiplication aging, so the strain more than 20 years should not carry out artificial pollination.
Early spring is suitably pruned to elite stand, prunes cella branch off, wipes out tree crown bottom deadwood and thin and delicate branch；In trees rudiment
Ground water is irrigated during lamina, with reference to application of organic fertilizers or the Chemical Mixed Fertilizer of watering, every plant apply 5kg~8kg (preferably 5kg) organic fertilizers or
1kg~2kg (preferably 1kg) Chemical Mixed Fertilizer is advisable.
Watering, fertilizing is carried out to male parent female parent trees, tree root is obtained sufficient nutrient and moisture, be conducive to blooming
With it is solid.
In step (3), harvesting the method for pollen is：Spray is gathered after paternal plant enters the early flowering season, before selection corolla
The Duan Mingxian bending flowers that vexil is not yet opened a business in sickle, collect flower pesticide.
Specifically method and step is：Spray is gathered after paternal plant enters the early flowering season, with averruncator or secateurs by spray
Cut and take interior, selection corolla leading portion substantially bends the flower that vexil is not yet opened a business in sickle, with scissors before corolla
Half is cut off in crook in 1/3rd, and petal is torn with hand, exposes yellow flower pesticide, flower pesticide is cut or carried off down with hand is placed on sulfuric acid
On paper, after collecting 90-110 flower, flower pesticide template is wrapped and is placed on dry 24 hours, opening paper bag in quick lime, used
Round and smooth hard-tipped pen bar gently rolls flower pesticide makes its rupture loose powder, and pollen is covered tightly into bottle cap in the vial of pollination device, places
Preserved in 0~4 DEG C of refrigerator of low temperature.
Using the benefit of template：Template papery is pure, intensity is high, transparent good, indeformable, sun-proof, high temperature resistant, anti-ageing
Change, the preservation effect to pollen is good.
By observation and experimental study, its flower growth maturity of the locust tree inflorescence of early flowering season is inconsistent, only reaches into
Ripe flower pollen just has vigor, and remaining immature flower pollen does not have sprouting vigor.Present invention selection corolla leading portion is obvious
Bending flower that vexil is not yet opened a business in sickle, this kind of pollen full maturity and with the ability of sprouting easily realizes pollination,
Improve the setting percentage of locust tree.
In step (4), specific method and step is：Inflorescence of suitably pollinating is selected on maternal plant, inflorescence is gently held, pinched
Flower, pushes the vexil of flower aside by tip tweezers, exposes stamen, pinches filigree upper end and carries off flower pesticide, and repetition is done 2~3 times,
Remove until by whole flower pesticide；Then style and column cap are gently stirred with tweezers, allows column cap to expose from petal, then taken rapidly
The pollination device alignment column cap pollination of adhesion pollen is played, pollen is stained with by gently hitting column cap, afterwards with tweezers by style and post
Head is sent back in petal, allows the style and column cap of pollination to reply physical slot, this completes 1 colored pollination；According to above-mentioned
Method then to the 2nd flower pollination, until all ripe flowers of whole inflorescence have all been awarded, is removed jejune flower, one
As each inflorescence pollinate flower quantity at 10~15, then give pollination inflorescence bagging.
Wherein, the suitably pollination inflorescence is：Flower grows to maturation, and corolla length no longer increases, and corolla leading portion is substantially curved
Folding is in sickle, and vexil is not yet opened a business, and now gynoecium is ripe, suitable pollination.Other undesirable flowers are from flower
Removed in sequence.By substantial amounts of verification experimental verification, the inflorescence of this condition is selected to be pollinated, its pollination success rate and setting percentage are significantly
Degree is improved.The preliminary experiment of artificial pollination it has also been found that flower itself development prematurity column cap in addition to this condition does not have mucus, or
It is very poor that flower have passed through florescence Pistil recipient phase.
It is described to pollination inflorescence bagging method be：The sprig of whole pollination inflorescence growth is all put into sack, is folded
Clutch sack to be rolled onto in last year life old branch, sack is fixed to clamp with branch with paper clip or clip, sack is not turned by wind-force
Move or wiped off by wind, sack makes of template, length 27cm, width 17cm, rectangle, one end open.
In step (5), pollination maternal plant management method is：Remove bagging within 5~7 days after pollination terminates, gently unfolded with hand
The branches and leaves of bagging, water in good time to pollination elite stand, spraying pesticide pest control (locust tree aphid and Robinia pseudoacacia Seeds chalcid fly), every 8
~12 days (preferably 10 days) spray once, continuous administration 2~3 times, the children of investigation records in the 40 days pod percentage of fertile fruit after pollination.
For pollination elite stand is watered in good time, spraying pesticide, pre- preventing disease and pest improves setting percentage.
In step (6), the ripe pod collecting method is：The ripe pod of harvesting in 80~90 days after pollination, earlier than this
Seed is immature in individual time pod, is later than this time and can increase the mouldy danger lost vigor to seed of pod microbiological contamination；It is accurate
Get kraft paper bag ready, ripe pod is cut and is put into paper bag, mark and hybridize Parent；By pod dryness in the sun a couple of days, dial
Open pericarp and take out seed, seed is put back in paper bag and is sealed, be placed on the indoor shady and cool place of drying and preserve.
The beneficial effects of the invention are as follows：
(1) what is had in the prior art is simple, easy in order to pollinate, and all extracts petal during emasculation, although side
Give column cap pollination, but lose its protective effect, disease pest harm is serious；What is had all pulls out stamen, but locust tree
Spend middle fossil fragments closely to include stamen and gynoecium, if stamen is all extracted, influence the growth position and growth hair of gynoecium
Educate；Conventional pollinating tool is to use writing brush, and it has pollen and is difficult adhesion, and the drawback such as pollen consumption is big.In face of prior art
Emasculation operating technology requirement height, pollination difficulty big problem when middle locust tree hybridizes, the invention provides a kind of side of locust tree emasculation
Method and a kind of pollination device, the method are easy, easy to operate, and will not damage petal and gynoecium, easily realize pollination, reduce gynoecium
Generation pest and disease damage improves crossbreeding fruit-setting rate.This is consistent with actual result of the test.
(2) method of the present invention easily realizes pollination, improves locust tree Natural seed setting rate；Improve locust tree artificial hybridization breeding
Success rate.The method can complete locust tree artificialpollination process, obtain hybrid seed；Locust tree artificial pollination can be significantly improved
Setting percentage, up to more than 20%；Improve locust tree artificial hybridization success rate of breeding.
Brief description of the drawings
Fig. 1 is the structural representation of pollination device of the invention.
Fig. 2 is the structural representation of rubber of the invention.
Fig. 3 is locust tree petal expanded view.
Fig. 4 is locust tree flower photo in full bloom.
Fig. 5 is the inflorescence and flower photo of suitable pollination.
Specific embodiment
With reference to specific embodiment, the present invention is described in detail.
Embodiment 1
The present invention provides a kind of locust tree artificial emasculation and hybridization controlled pollination method, comprises the following steps：
(1) pollination device is made：Rubber 4 is cut into 2cm long, the cylinder of diameter 1cm, will wherein one sharpening, for gluing
Pollen, other end is scabbled, and in a piece thin wire 3 of centre insertion, is connected with the bottle closure of rubber 2 in vial, thin wire length
About 10cm, is shown in Fig. 1 and Fig. 2；Wherein vial height 5cm, diameter 2cm, cylindrical rubber bottle stopper.
(2) parent sire of hybrid pigs and maternal plant are selected, selection enters the locust tree improved seeds Middle and young forest 6 of generative growth phase
Raw healthy and strong tree is used as hybridization Parent plant within~20 years.Early spring is suitably pruned to elite stand, prunes cella branch off, is wiped out under tree crown
Portion's deadwood and thin and delicate branch.Ground water is irrigated in trees Germination to Frondescent, with reference to watering using appropriate organic fertilizer or Chemical Mixed Fertilizer, every plant is applied
It is advisable with 5kg organic fertilizers or 1kg trees (fruit tree) compound fertilizer special.
(3) pollen is harvested, spray is gathered after paternal plant enters the early flowering season, will taken under flower twig clippers with averruncator or secateurs
To interior, the obvious bending flower that vexil is not yet opened a business in sickle of selection corolla leading portion, with scissors three before corolla/
Half is cut off in crook in one, and petal is torn with hand, exposes yellow flower pesticide, flower pesticide is cut or carried off down with hand is placed on template,
After collecting certain amount (about 100) flower, flower pesticide template is wrapped and is placed on dry 24 hours, opening paper in quick lime
Bag, gently rolling flower pesticide with round and smooth hard-tipped pen bar makes it rupture loose powder, and pollen is covered tightly into bottle cap in the vial of pollination device,
It is placed on (0~4 DEG C) storage of low temperature in refrigerator；
(4) artificial pollination, (inflorescence of suitably pollinating refers to that flower grows into the inflorescence that selects suitably to pollinate on maternal plant
Ripe, corolla length no longer increases, and substantially bending is in sickle to corolla leading portion, and vexil is not yet opened a business, and now gynoecium is ripe,
Suitable pollination), left hand gently holds inflorescence, and flower is pinched with forefinger and thumb, and the right hand takes tip tweezers to push the vexil of flower aside (figure
3 is locust tree petal expanded view), expose stamen, pinch filigree upper end and carry off flower pesticide, repetition is done 2~3 times, until by whole flowers
Medicine removes.Then style and column cap are gently stirred with tweezers, allows column cap to expose from petal, adhesion pollen is then picked up rapidly
Pollination device alignment column cap pollination, is stained with pollen by gently hitting column cap, afterwards sent in petal back to style and column cap with tweezers,
The style and column cap of pollination is allowed to reply physical slot, this completes 1 pollination of flower.According to the method described above then to the
2 flower pollinations, until all ripe flowers of whole inflorescence have all been awarded, remove jejune flower, general each inflorescence
Pollination flower quantity is at 10~15.Then pollination inflorescence bagging is given, maternal and male parent title is marked on sack, flower of pollinating
Quantity, pollination date.Label is hung to pollination inflorescence sprig simultaneously, maternal and male parent title, date and pollination people is marked；To pollination
The method of inflorescence bagging is that the sprig of whole inflorescence growth of pollinating all is put into sack, and folding is clutched sack and is rolled onto last year life always
On branch, sack is fixed to clamp with branch with paper clip or small folder, sack is not wiped off by turned or by wind.Sack sulphur
Sour paper makes, length 27cm, width 17cm, rectangle, one end open.
(5) management of pollination maternal plant, removes bagging in 5~7 days after pollination terminates, and the branches and leaves of bagging are gently unfolded with hand, unites
Count the inflorescence to bear fruit flower quantity, record pollination flower quantity.Watered in good time to pollination elite stand, spraying pesticide preventing and treating locust tree aphid
(Aphis robiniae) and Robinia pseudoacacia Seeds chalcid fly (Bruchophagus philorobiniae), every sprinkling in 10 days once,
Continuous administration 2~3 times.The children of investigation records in 40 days or so the pod percentage of fertile fruit after pollination.
(6) ripe pod harvesting is hybridized, the ripe pod of harvesting in 80~90 days, plants earlier than in this time pod after pollination
Son is immature, is later than this time and can increase the mouldy danger lost vigor to seed of pod microbiological contamination.Kraft paper bag is got out, will
Ripe pod is cut and is put into paper bag, marks and hybridizes Parent.By several days of pod dryness in the sun, push pericarp aside and take out seed,
Seed is put back in paper bag and is sealed, be placed on the indoor shady and cool place of drying and preserve.
Embodiment 2
Conventional locust tree artificial pollination method is that the inflorescence that selection maternal plant suitably pollinates gives flower emasculation, and flower is cut with scissors
The petal of middle and upper part is preced with, exposes stamen and gynoecium, then cut stamen, or flower pesticide is carried off with hand, dipping in a little pollen with writing brush applies
Column cap is smeared, column cap pollination is given.Bagging hangs label after completing the pollination of whole inflorescence.
Experimental example 1
The method of Application Example 1 in 2014 constitutes cross combination to locust tree different clones, implements artificialpollination,
As a result, Shandong thorn 57 × 15 cross combination setting percentages of emerging thorn are 21.28%, the setting percentage of 1 × Shandong of emerging thorn thorn 68 is 16.82%,
The setting percentage that 152 × Shanxi thorn 4 is pierced in Shandong is 13.05%.It is shown in Table 1.
Experimental example 2
Grope within 2013 to constitute cross combination to locust tree different clones using the method first, implement artificial control and award
Powder, as a result, Shandong thorn 57 × 15 cross combination setting percentages of emerging thorn are 5.91%, the setting percentage of 1 × Shandong of emerging thorn thorn 68 is 4.41%,
The setting percentage that 152 × Shanxi thorn 4 is pierced in Shandong is 4.32%.It is shown in Table 1.
Experimental example 3
Cross combination was constituted to locust tree different clones using conventional method (embodiment 2) in 2012, implement artificial control
Pollination, as a result, Shandong thorn 57 × 15 cross combination setting percentages of emerging thorn are 1.36%, the setting percentage of 1 × Shandong of emerging thorn thorn 68 is
0.47%, the setting percentage that 152 × Shanxi thorn 4 is pierced in Shandong is 0.49%.It is shown in Table 1.
The different year Robinia Pseudoacacia Clones cross combination artificial pollination setting percentages of table 1
Claims (5)
1. a kind of locust tree artificial emasculation and hybridization controlled pollination method, it is characterized in that, comprise the following steps：
（1）Make pollination device：The pollination device includes containing polliniferous vial, and vial is closed using bottle closure of rubber, described
Bottle closure of rubber in vial is connected by wire with rubber；
（2）Selected parent sire of hybrid pigs and maternal plant；
Selecting the method for parent sire of hybrid pigs and maternal plant is：Selection enters the locust tree improved seeds Middle and young forest of generative growth phase
Raw healthy and strong tree is used as hybridization Parent plant within 6 ~ 20 years；Early spring is suitably pruned to elite stand, prunes cella branch off, is wiped out under tree crown
Portion's deadwood and thin and delicate branch；Ground water is irrigated in trees Germination to Frondescent, with reference to application of organic fertilizers or the Chemical Mixed Fertilizer of watering, every plant of administration 5 ~
8kg organic fertilizers or 1 ~ 2kg Chemical Mixed Fertilizers；
（3）Harvesting pollen；
Harvesting the method for pollen is：Spray is gathered after paternal plant enters the early flowering season, substantially bending is in sickle to selection corolla leading portion
Blade type and flower that vexil is not yet opened a business, collect flower pesticide；
（4）Emasculation, pollination：
Specifically method and step is：Inflorescence of suitably pollinating is selected on maternal plant, inflorescence is gently held, flower is pinched, by fine-pointed forceps
Son pushes the vexil of flower aside, exposes stamen, pinches filigree upper end and carries off flower pesticide, and repetition is done 2~3 times, until by whole flowers
Medicine removes；Then style and column cap are gently stirred with tweezers, allows column cap to expose from petal, adhesion pollen is then picked up rapidly
Pollination device alignment column cap pollination, is stained with pollen by gently hitting column cap, afterwards sent in petal back to style and column cap with tweezers,
The style and column cap of pollination is allowed to reply physical slot, each inflorescence is pollinated flower quantity at 10~15, spent to pollination after pollination
Sequence bagging；
It is described suitably pollination inflorescence be：Flower grows to maturation, and corolla length no longer increases, and substantially bending is in reaping hook to corolla leading portion
Type, and vexil is not yet opened a business, now gynoecium is ripe, suitable pollination, and other undesirable flowers are removed from inflorescence；
（5）Pollination maternal plant management；
（6）The ripe pod harvesting of hybridization；
The ripe pod collecting method is：The ripe pod of harvesting in 80~90 days after pollination；Kraft paper bag is got out, by maturation
Pod is cut and is put into paper bag, marks and hybridizes Parent；By pod dryness in the sun a couple of days, push pericarp aside and take out seed, will plant
Son puts back to sealing in paper bag, is placed on the indoor shady and cool place of drying and preserves.
2. the method for claim 1, it is characterized in that：Step（1）In, the rubber is connected for a head sharpening other end
The cylindrical type rubber of wire.
3. the method for claim 1, it is characterized in that：Step（3）In, specific method and step is：Enter in paternal plant
Spray is gathered after entering the early flowering season, interior will be taken under flower twig clippers with secateurs, selection corolla leading portion substantially bends in sickle flag
The flower that valve is not yet opened a business, with scissors 1/3rd before the corolla in crook cut off half, tear petal with hand, expose yellow flower
Medicine, flower pesticide cut or carried off down with hand be placed on template, after collecting 90-110 flower, flower pesticide template is wrapped and is placed on
Dried 24 hours in quick lime, open paper bag, gently rolling flower pesticide with round and smooth hard-tipped pen bar makes it rupture loose powder, and pollen is mounted in
Bottle cap is covered tightly in the vial of pollination device, storage in 0~4 DEG C of refrigerator of low temperature is placed on.
4. the method for claim 1, it is characterized in that：Step（4）In, the method to pollination inflorescence bagging is：Will
The sprig of whole pollination inflorescence growth all puts into sack, and folding is clutched sack and is rolled onto in last year life old branch, with paper clip or folder
Son fixes to clamp sack with branch, sack is not wiped off by turned or by wind, and sack makes of template.
5. the method for claim 1, it is characterized in that：Step（5）In, pollination maternal plant management method is：Terminate in pollination
Remove bagging within 5~7 days afterwards, the branches and leaves of bagging are gently unfolded with hand, watered in good time to pollination elite stand, spraying pesticide pest control,
Every sprinkling in 8 ~ 12 days once, continuous administration 2~3 times.
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| CN109105251A (en) * | 2018-08-22 | 2019-01-01 | 张东风 | A method of improving grape artificial pollination success rate |
| CN109287352B (en) * | 2018-09-04 | 2020-12-29 | 山东省林业科学研究院 | Method for improving fruit setting rate of artificial control pollination of elderberry |
| CN109197579A (en) * | 2018-11-26 | 2019-01-15 | 安徽泓森高科林业股份有限公司 | Deep gloomy Chinese scholartree crossbreeding technology |
| CN111771659B (en) * | 2020-08-04 | 2022-02-22 | 广西壮族自治区农业科学院 | Method for improving fruiting rate of jasmine flowers and pollination device thereof |
| CN112544439A (en) * | 2020-12-28 | 2021-03-26 | 江苏省农业科学院 | Exquisite tomato cross pollination method |
| CN114402992A (en) * | 2021-12-21 | 2022-04-29 | 黑龙江省林业科学研究所 | Hybrid pollination method for lonicera caerulea |
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Non-Patent Citations (4)
| Title |
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| "22-66"花粉授粉器;张学明;《农业科技通讯》;19790630(第6期);全文 * |
| 刺槐开花生物学特性观察;原法宪;《山西林业科技》;19780630(第3期);全文 * |
| 刺槐开花结实生物学特性的观察初报;田志和;《辽宁林业科技》;19810501(第2期);全文 * |
| 种子园刺槐开花和控制授粉的初步研究;解荷锋;《山东林业科技》;19940822(第4期);第5页2.2控制授粉 * |
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