CN114402992A - Hybrid pollination method for lonicera caerulea - Google Patents
Hybrid pollination method for lonicera caerulea Download PDFInfo
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- CN114402992A CN114402992A CN202111572359.2A CN202111572359A CN114402992A CN 114402992 A CN114402992 A CN 114402992A CN 202111572359 A CN202111572359 A CN 202111572359A CN 114402992 A CN114402992 A CN 114402992A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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Abstract
The invention discloses a method for cross pollination of lonicera caerulea, which comprises the following steps: collecting flowers of a male parent which just bloom for 1 day, placing the flowers with exposed stamens in a culture dish, placing the flowers in a room, shearing anthers, placing the anthers on the culture dish, drying in the shade, and storing the obtained pollen for 1-3 days at normal temperature; selecting flowers which are not opened on strong branches and have unexposed pistils as pollination parent objects, gradually growing flower columns of lonicera caerulea in buds, cutting off the stamens before the buds are pushed open, and only leaving the pistils; manufacturing a pollination tool, and sticking pollen by using an eraser to pollinate pistils; and (3) coating a sulfuric acid paper bag after pollination, observing the development process of the fruits, removing the sulfuric acid paper bag after 15 days, changing the sulfuric acid paper bag into a mesh bag, and falling into the paper bag after the fruits are mature. By adopting the method for cross pollination of the lonicera caerulea, part of operation links in pollination are improved, the work efficiency of pollination is improved, the fruit setting rate of the lonicera caerulea is improved, and the quality of fruits and seeds is improved.
Description
Technical Field
The invention relates to the technical field of hybrid seed production, in particular to a method for hybrid pollination of lonicera caerulea.
Background
Lonicera edulis, etc. The fruit is rich in nutrition, has high value, is rich in Vc and amino acid, contains 7 kinds of Lonicera edulis in 8 kinds of amino acid which can not be synthesized by human body, and is also rich in various trace elements which are indispensable to human body, especially the highest content of magnesium, potassium and iron. Besides high edible value, the fruit also contains abundant VP active substances, such as rutin, anthocyanin, etc., and has high medicinal value. Research shows that the fruit not only has the efficacy of clearing away heat and toxic material, but also has certain curative effects on reducing blood pressure, increasing the number of blood cells and treating infantile anorexia. Therefore, the development and utilization of the indigo fruit resources are profound. The wild lonicera caerulea has bad taste and is sour and astringent, and is easily polluted by other pollen in the pollination process. In view of the above, it is necessary to design a method for cross pollination of lonicera caerulea to improve variety.
Disclosure of Invention
The invention aims to provide a method for cross pollination of lonicera edulis, which improves part of operation links in pollination, greatly improves the work efficiency of pollination, avoids pollution of other pollen, improves the fruit setting rate of lonicera edulis, improves the taste of fruits and the quality of seeds, has low cost in the implementation process and is easy to realize.
In order to achieve the above object, the present invention provides a method for cross pollination of lonicera caerulea, comprising the steps of:
s1, selecting a hybridization male parent and a hybridization female parent;
s2, collecting flowers which just bloom for 1 day on the male parent, placing the flowers with the stamens exposed and extended into a culture dish, then placing the flowers indoors, shearing the anthers with a small ophthalmic scissors, placing the anthers on the culture dish, drying the anthers in the shade, and then storing the obtained pollen for 1-3 days at normal temperature;
s3, selecting flowers which are not opened on strong branches and have unexposed pistils as pollination parent objects, gradually growing stigmas of lonicera caerulea in buds, and cutting off the pistils and only leaving the pistils before the buds are pushed open;
s4, rotating the pointed end of the pin 180 degrees around the base, pricking a cubic rubber block with the side length of 0.5cm to manufacture a pollination tool, and using an eraser to pick pollen to pollinate pistils;
s5, sleeving a 7 x 10cm sulfuric acid paper bag after pollination, observing the development process of the fruits, removing the sulfuric acid paper bag after 15 days, changing the sulfuric acid paper bag into a mesh bag, and falling into the paper bag after the fruits are mature.
Preferably, the lonicera caerulea is selected from the clone No. 6 of lonicera caerulea.
Preferably, the lonicera caerulea selects mixed pollen of clones MX4, HZ6, FH9 and BL 11.
Therefore, by adopting the method for cross pollination of the lonicera edulis, the invention improves part of operation links in pollination, greatly improves the work efficiency of pollination, avoids pollution of other pollen, improves the fruit setting rate of the lonicera edulis, improves the taste of fruits and the quality of seeds, has low cost in the implementation process and is easy to realize.
The technical solution of the present invention is further described in detail by the following examples.
Detailed Description
The invention provides a method for cross pollination of lonicera caerulea, which comprises the following steps:
s1, selecting a hybridization male parent and a hybridization female parent;
s2, collecting flowers which just bloom for 1 day on the male parent, placing the flowers with the stamens exposed and extended into a culture dish, then placing the flowers indoors, shearing the anthers with a small ophthalmic scissors, placing the anthers on the culture dish, drying the anthers in the shade, and then storing the obtained pollen for 1-3 days at normal temperature;
s3, selecting flowers which are not opened on strong branches and have unexposed pistils as pollination parent objects, gradually growing stigmas of lonicera caerulea in buds, and cutting off the pistils and only leaving the pistils before the buds are pushed open;
s4, rotating the pointed end of the pin 180 degrees around the base, pricking a cubic rubber block with the side length of 0.5cm to manufacture a pollination tool, and using an eraser to pick pollen to pollinate pistils;
s5, sleeving a 7 x 10cm sulfuric acid paper bag after pollination, observing the development process of the fruits, removing the sulfuric acid paper bag after 15 days, changing the sulfuric acid paper bag into a mesh bag, and falling into the paper bag after the fruits are mature.
The technical solution of the present invention is further illustrated by the following examples.
Example 1
The artificial pollination is carried out by using pollen preserved for 1 day at normal temperature, 15 plants of clone No. 6 lonicera caerulea are selected as female parents for artificial pollination, and mixed pollination is designed for avoiding the influence caused by the affinity difference between different male parents and female parents, wherein mixed pollen of clone No. MX4, clone No. HZ6, clone No. FH9 and clone No. BL11 is selected and preserved for 1 day at normal temperature, and then mixed pollination is carried out. Pollinating 30 female flowers repeatedly by using mixed pollen pollination, and repeating for 3 times.
Example 2
The pollen preserved for 3d at normal temperature is used for artificial pollination, 15 plants of the clone No. 6 of the lonicera caerulea are selected as female parents for artificial pollination, and the mixed pollen preserved for 3d at normal temperature and from the same source as the mixed pollen in the example 1 is selected for artificial pollination. Pollinating 30 female flowers repeatedly by using mixed pollen pollination, and repeating for 3 times.
Comparative example
15 plants of clone No. 6 Lonicera caerulea were selected without any pollination and allowed to pollinate naturally as a control.
The final fruit setting rate before fruit harvest is counted as the number of mature fruits/pollinated flowers multiplied by 100%.
After the fruit was harvested, the transverse and longitudinal diameters of the fruit were measured with vernier calipers and the weight of the fruit was weighed with an electronic balance.
The test results are as follows:
the artificial pollination obviously improves the fruit setting rate of the lonicera caerulea. Wherein, the artificial pollination is carried out by using the pollen preserved for 1d and the pollen preserved for 3d, so that the fruit setting rate is 12 percent and 8.80 percent higher than that of the natural pollination respectively. The natural pollination rate of the lonicera caerulea is about 72 percent generally, which shows that the fruit yield can be obviously improved by artificial pollination, and the pollen preserved for 1 day at normal temperature has higher activity and better yield increasing effect.
Artificial pollination has a significant effect on fruit weight, fruit transverse diameter and fruit longitudinal diameter. Compared with natural pollination, artificial pollination is carried out by using pollen preserved at normal temperature for 1d and 3d, so that the weight of fruits is increased by 20.20% and 12.82%, the transverse diameter of the fruits is increased by 11.04% and 7.58%, the longitudinal diameter of the fruits is increased by 10.84% and 5.44%, and the number of the fruits with good taste is increased by 26% and 21.5%.
The pollen preserved at normal temperature for 1 day has better activity and better pollination effect, and the artificial pollination can obviously improve the yield of the lonicera caerulea fruit. The artificial pollination can select the pollen with the highest activity, and the effective pollination is carried out at the time when the stigma pollination property is the best, so the pollination fertility rate is higher, the pollination fertilization quality is good, the growing fruit is larger, and the fruit yield is improved.
Therefore, by adopting the method for cross pollination of the lonicera edulis, the invention improves part of operation links in pollination, greatly improves the work efficiency of pollination, avoids pollution of other pollen, improves the fruit setting rate of the lonicera edulis, improves the taste of fruits and the quality of seeds, has low cost in the implementation process and is easy to realize.
Finally, it should be noted that: the above embodiments are only for illustrating the technical solutions of the present invention and not for limiting the same, and although the present invention is described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that: modifications and equivalents may be made to the invention without departing from the spirit and scope of the invention.
Claims (3)
1. A method for cross pollination of lonicera caerulea is characterized by comprising the following steps:
s1, selecting a hybridization male parent and a hybridization female parent;
s2, collecting flowers which just bloom for 1 day on the male parent, placing the flowers with the stamens exposed and extended into a culture dish, then placing the flowers indoors, shearing the anthers with a small ophthalmic scissors, placing the anthers on the culture dish, drying the anthers in the shade, and then storing the obtained pollen for 1-3 days at normal temperature;
s3, selecting flowers which are not opened on strong branches and have unexposed pistils as pollination parent objects, gradually growing stigmas of lonicera caerulea in buds, and cutting off the pistils and only leaving the pistils before the buds are pushed open;
s4, rotating the pointed end of the pin 180 degrees around the base, pricking a cubic rubber block with the side length of 0.5cm to manufacture a pollination tool, and using an eraser to pick pollen to pollinate pistils;
s5, sleeving a 7 x 10cm sulfuric acid paper bag after pollination, observing the development process of the fruits, removing the sulfuric acid paper bag after 15 days, changing the sulfuric acid paper bag into a mesh bag, and falling into the paper bag after the fruits are mature.
2. The method for cross pollination of lonicera caerulea according to claim 1, wherein the steps of: the Lonicera edulis is selected from Lonicera edulis clone No. 6.
3. The method for cross pollination of lonicera caerulea according to claim 2, wherein the steps of: the Lonicera caerulea selects mixed pollen of clone MX4, clone HZ6, clone FH9 and clone BL 11.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202111572359.2A CN114402992A (en) | 2021-12-21 | 2021-12-21 | Hybrid pollination method for lonicera caerulea |
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| CN202111572359.2A CN114402992A (en) | 2021-12-21 | 2021-12-21 | Hybrid pollination method for lonicera caerulea |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104082129A (en) * | 2014-07-29 | 2014-10-08 | 安徽省农业科学院园艺研究所 | Pollinating method for pomegranate crossbreeding |
| CN105409758A (en) * | 2015-11-13 | 2016-03-23 | 山东省林业科学研究院 | Artificial emasculation and hybrid control pollination method of robinia pseudoacacia L. |
| CN111418489A (en) * | 2020-04-03 | 2020-07-17 | 北京林业大学 | Artificial cross pollination method for xanthoceras sorbifolia bunge |
-
2021
- 2021-12-21 CN CN202111572359.2A patent/CN114402992A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104082129A (en) * | 2014-07-29 | 2014-10-08 | 安徽省农业科学院园艺研究所 | Pollinating method for pomegranate crossbreeding |
| CN105409758A (en) * | 2015-11-13 | 2016-03-23 | 山东省林业科学研究院 | Artificial emasculation and hybrid control pollination method of robinia pseudoacacia L. |
| CN111418489A (en) * | 2020-04-03 | 2020-07-17 | 北京林业大学 | Artificial cross pollination method for xanthoceras sorbifolia bunge |
Non-Patent Citations (4)
| Title |
|---|
| ALES VALDEK等: "Pollination and Pollinators of haskap(Lonicera caerulea)", 《MENDELNET》 * |
| 李春野等: "优质晚熟安哥诺李引种栽培表现及管理技术", 《果农之友》 * |
| 李红莉等: "蓝靛果忍冬杂交亲和力及杂交浆果性状分析", 《种子》 * |
| 焦丽等: "蓝靛果忍冬杂交育种试验初步研究", 《防护林科技》 * |
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Application publication date: 20220429 |