CN105409758A - Artificial emasculation and hybrid control pollination method of robinia pseudoacacia L. - Google Patents
Artificial emasculation and hybrid control pollination method of robinia pseudoacacia L. Download PDFInfo
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- CN105409758A CN105409758A CN201510783041.7A CN201510783041A CN105409758A CN 105409758 A CN105409758 A CN 105409758A CN 201510783041 A CN201510783041 A CN 201510783041A CN 105409758 A CN105409758 A CN 105409758A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
- A01H1/027—Apparatus for pollination
Abstract
The invention discloses an artificial emasculation and hybrid control pollination method of robinia pseudoacacia L.. The method comprises the following steps of pollenator making, selection of hybrid male parent and female parent plants, pollen collecting, artificial pollination, pollinated female plant management and hybrid ripe pod harvesting. According to the artificial emasculation and hybrid control pollination method of the robinia pseudoacacia L., the technical steps of emasculation and pollination for the robinia pseudoacacia L. are described in detail, petals and pistil are not damaged, the artificial pollination ripening rate of the robinia pseudoacacia L. can be significantly increased, and the artificial hybridization breeding success rate of the robinia pseudoacacia L. is increased.
Description
Technical field
The invention belongs to field of plant variety breeding technology, be specifically related to a kind of locust tree artificial emasculation and hybridization controlled pollination method.
Background technology
Locust tree (RobiniapseudoacaciaL.) is pulse family Papillionoideae robinia broad-leaved megaphanerophyte, and originate in the U.S., at the beginning of the 17th century, (1601) are introduced into Europe, spread all over European Countries to 18th century.Locust tree provenance is introduced Chinese Qingdao by German by 19 end of the centurys first, several the locust tree provenances from Japan, the U.S., Korea are had again 40 ~ sixties of 20th century to be planted in the ground such as China east area of the Liao River, coextensive with eastern and southern Liaoning Province, Tianshui city, Changsha and North China, to late 1980s, China has 24 provinces, municipalities and autonomous regions to plant locust tree, only 6 provinces such as Hebei, Henan, Shandong, Shanxi, Liaoning just reach 4,000,000,000 strains, become China North China, northwest, THE SOUTH OF NORTHEAST CHINA, the main timber forest of all provinces of Yangtze-Huaihe River Valley, firewood forest, soil and water conservation forest, sea wall and river levee shelter forest seeds.Locust tree is thick with leaves, and the flowers are in blossom, and fragrance is strong, is high-quality nectar source seeds; Locust tree timber is tough and tensile, shock resistance, resistance to rotten, water-fast wet, be important building, ore pillar, vehicle, shipbuilding material; Locust tree timber calorific value is high, and burning velocity is slow, is high-quality fire wood and excellent Tree Species as Bio-energy; Locust tree greenery nutrient composition content is high, nonpoisonous and tasteless, is first-class animal feeding-stuff.Locust tree is as multivalence value, multipurpose, good stress resistance, good seeds that suitable raw ability is strong, and the application prospect of its improved Varieties is very wide.
Locust tree breeding is both at home and abroad mainly based on selection and use technology for a long time, few employing artificial hybridization breeding technique.Selection and use technology is discovery, selects and utilize natural excellent genetic variation individual plant or type, and method is simple, and cost is lower, but according to artificial wish determination breeding objective, more can not can not determine hybrid strain from main separation.Artificial hybridization breeding technique can according to artificial wish, and select the hybridization Parent being applicable to breeding objective, artificialpollination process, obtains super close hybrid generation.By the mensuration to hybrid generation, screening and utilization, obtain improved Varieties.
Locust tree is the same flowering plant of monoecism, hermaphrodite flower.Raceme, the crown of papilionaceous flower, 5 pieces, petal, is divided into 1 piece of vexil, 2 pieces of alas, 2 pieces of keel, as shown in Figure 4.Order of blooming is that vexil is first opened a business, and rear 2 alas are opened a business, and gynoecium is sickle by 2 keel together with stamen inclusion, until vexil and the withered decline of ala are not also opened a business.So from the appearance less than flower pesticide and column cap when Pagoda Flower is open, need pushes petal aside just can expose stamen and gynoecium by instrument, this brings great difficulty to crossbreeding artificial pollination.Artificial pollination should remove stamen, and also will push petal aside and expose column cap, this brings damage to flower, affects its normal physiological and biochemical procedure, makes artificial pollination ripening rate lower than Natural seed setting rate.Locust tree Natural seed setting rate is lower, and average 30%, this makes locust tree artificial hybridization success rate of breeding be restricted.Our result of the test is, adopts conventional method to its ripening rate of locust tree artificialpollination 1% ~ 5%, in addition document " locust tree blooms and pollinates and mating pattern " Journal of northeast Forestry university, and open artificial emasculation is hybridized average fruit-setting rate and is only 3.56%; The relevant locust tree artificial pollination ripening rate of hitherto reported is also within the scope of this.
Summary of the invention
For current crossbreeding technology Problems existing, the invention provides a kind of to Pagoda Flower emasculation, pollination, and do not damage the method for petal and gynoecium, and invent a kind of pollenator.Utilize the method and pollenator can complete locust tree artificialpollination process, obtain hybrid seed.
A kind of locust tree artificial emasculation and hybridization controlled pollination method, comprise the following steps:
(1) pollenator is made: described pollenator comprises contains polliniferous vial, and vial adopts bottle closure of rubber to close, and the bottle closure of rubber in described vial is connected with rubber by wire;
(2) selected parent sire of hybrid pigs and maternal plant;
(3) to gather pollen;
(4) emasculation, pollination: select pollination inflorescence maternal plant, hold inflorescence, pinch flower, the vexil of flower is pushed aside, exposes stamen, pinch filigree upper end and flower pesticide is removed; Stir style and column cap after emasculation, allow column cap expose in petal, then adopt the pollenator of adhesion pollen to aim at column cap pollination, with tweezers, style and column cap are sent back in petal afterwards, allow the style of pollination and column cap recover physical slot;
(5) maternal plant that pollinates manages;
(6) hybridize ripe pod to gather.
In step (1), the present invention, according to the growth characteristic of locust tree inflorescence, designs a kind of pollenator of applicable Pagoda Flower, comprises containing polliniferous vial and being the cylinder shape rubber of a head sharpening other end connection metal silk.
The preparation method of described pollenator is, rubber is cut into long 1.8 ~ 2.2cm (preferred 2cm), the cylinder of diameter 0.8 ~ 1.2cm (preferred 1cm), to a wherein sharpening, for sticky pollen, other end is scabbled, insert an one metal wire in centre, be connected with the bottle closure of rubber in vial, thin wire length 5 ~ 10cm (preferred 10cm).
Wherein vial height 4 ~ 6cm (preferred 5cm), diameter 1.5 ~ 2.5cm (preferred 2cm), cylindrical rubber bottle stopper.
Described pollenator is that applicant gropes through long-term practice, and use during its most applicable Pagoda Flower pollination, its operating efficiency is all higher with pollination success rate.
The employing writing brush had in prior art is as pollenator, but the effect that pollen smeared by locust tree employing writing brush is unsatisfactory, according to the physilogical characteristics of Pagoda Flower and the character of locust tree pollen, the present invention adopts above-mentioned rubber pollenator, easy, easy to operate, through a large amount of verification experimental verifications, gynoecium pollination success rate and efficiency high, do not damage petal and gynoecium.
Select rubber to make pollenator and have four advantages, one is soft, the flexible and toughness of rubber quality, is easy to iron wire and inserts and fix; Two is be easy to pollen of adhering; Three is make easily, and cost is low; Four, the bottle closure of rubber in vial is combined makes pollenator, and pollen contamination rate is little, and pollination efficiency improves.
In step (2), the method for selected parent sire of hybrid pigs and maternal plant is: select locust tree improved seeds Middle and young forest 6 ~ 20 years raw healthy and strong trees entering generative growth phase as hybridization Parent plant.
Select the strain of the age of tree 6 ~ 20 years growing way stalwartnesses as hybridization Parent plant, the locust tree lower than 6 years age of trees enters generative growth phase not yet completely due to himself, should not implement artificial pollination; More than the strain of life in 20 years due to self growth and nutritive peculiarity, cause the problems such as organ of multiplication is aging, so the strain more than 20 years should not carry out artificial pollination.
Early spring suitably prunes elite stand, prunes cella branch off, wipes out tree crown bottom deadwood and thin and delicate branch; Irrigate ground water when trees Germination to Frondescent, in conjunction with application of organic fertilizers or the Chemical Mixed Fertilizer of watering, 5kg ~ 8kg (preferred 5kg) fertilizer is used in every strain or 1kg ~ 2kg (preferred 1kg) Chemical Mixed Fertilizer is advisable.
Watering, fertilizing is carried out to the maternal trees of male parent, makes tree root obtain sufficient nutrient and moisture, be conducive to blooming with solid.
In step (3), the method for pollen of gathering is: after paternal plant enters the early flowering season, gather spray, selects corolla leading portion obviously to bend the flower that vexil is not yet opened a business in sickle, collects flower pesticide.
Concrete method step is: after paternal plant enters the early flowering season, gather spray, indoor are taken by under flower twig clippers with averruncator or secateurs, corolla leading portion is selected obviously to bend the flower that vexil is not yet opened a business in sickle, with scissors corolla first three/mono-in crook cut off half, petal is torn with hand, expose yellow flower pesticide, being cut by flower pesticide or carrying off with hand is placed down on template, after collecting 90-110 flower, flower pesticide template is wrapped and to be placed in quicklime dry 24 hours, open paper bag, roll flower pesticide gently with round and smooth hard-tipped pen bar and make its loose powder of breaking, pollen is contained in the vial of pollenator and covers tightly bottle cap, be placed in the refrigerator of 0 ~ 4 DEG C of low temperature and preserve.
Adopt the benefit of template: template papery is pure, intensity is high, transparent good, indeformable, sun-proof, high temperature resistant, anti-aging, good to the preservation effect of pollen.
Through observation and experimental study, its flower growth maturity of the locust tree inflorescence of early flowering season is inconsistent, only reaches ripe flower pollen and just has vigor, and vigor sprouted by all the other immature flower pollen not tool.The present invention selects corolla leading portion obviously to bend the flower that vexil is not yet opened a business in sickle, this kind of pollen full maturity and have sprouting ability, easily realizes pollination, improves the ripening rate of locust tree.
In step (4), concrete method step is: select to be suitable for inflorescence of pollinating on maternal plant, gently hold inflorescence, pinch flower, push aside by the vexil of tip tweezers by flower, expose stamen, pinch filigree upper end flower pesticide is carried off, repeat to do 2 ~ 3 times, until whole flower pesticide is removed; Then style and column cap is stirred gently with tweezers, column cap is allowed to expose in petal, then the pollenator picking up rapidly adhesion pollen aims at column cap pollination, hit column cap gently and can be stained with pollen, with tweezers, style and column cap are sent back in petal afterwards, allow the style of pollination and column cap reply physical slot, this completes the pollination of 1 flower; Then give the 2nd flower pollination according to the method described above, until all ripe flowers of whole inflorescence have all been awarded, jejune flower is removed, general each inflorescence pollination flower quantity, at 10 ~ 15, then gives pollination inflorescence bagging.
Wherein, the described inflorescence that is suitable for pollinating is: flower grows to maturation, and corolla length no longer increases, and corolla leading portion obviously bends in sickle, and vexil is not yet opened a business, and now gynoecium is ripe, suitable pollination.Other undesirable flowers are removed from inflorescence.Through a large amount of verification experimental verifications, select the inflorescence of this condition to pollinate, its pollination success rate and ripening rate increase substantially.The flower self that the preliminary experiment of artificial pollination also finds in addition to this condition is grown prematurity column cap and is not had mucus, or flower to have have passed through flowering stage Pistil recipient phase very poor.
The described method of pollination inflorescence bagging of giving is: the sprig of whole pollination inflorescence growth is all put into sack, folding sack of clutching is rolled onto on raw old branch last year, with paper clip or clip, sack and branch are fixed to clamp, make sack not by wind rotate or wiped off by wind, sack template makes, length 27cm, width 17cm, rectangle, one end open.
In step (5), pollination maternal plant management method is: within 5 ~ 7 days after pollination terminates, remove bagging, the branches and leaves of bagging are gently unfolded with have gentle hands, water to pollination elite stand in good time, spraying pesticide pest control (locust tree aphid and Robinia pseudoacacia Seeds chalcid fly), spray once every 8 ~ 12 days (preferably 10 days), continuous administration 2 ~ 3 times, at pollination investigation records in latter 40 days children pod percentage of fertile fruit.
For pollination elite stand is watered in good time, spraying pesticide, pre-preventing disease and pest, improves ripening rate.
In step (6), described ripe pod collecting method is: 80 ~ 90 days after pollination ripe pods of gathering, immature early than seed in this time pod, is later than this time can increase the mouldy danger lost vigor to seed of pod microbiological contamination; Get out kraft paper bag, ripe pod is cut and is put in paper bag, mark and hybridize Parent; By pod dryness in the sun a couple of days, push pericarp aside and take out seed, seed is put back in paper bag and seals, be placed on indoor shady and cool dry place storage.
The invention has the beneficial effects as follows:
(1) what have in prior art is simple, easy in order to pollinate, and is all extracted by petal in emasculation process, although facilitate to column cap pollination, loses its protective effect, and sick worm harm is serious; What have all pulls out stamen, but in Pagoda Flower, keel, by stamen and the tight inclusion of gynoecium, if all extracted by stamen, affect the growth position of gynoecium and grow; Conventional pollinating tool is that it exists pollen and not easily adheres with writing brush, and the drawback such as pollen consumption is large.In prior art, during locust tree hybridization, emasculation operating technology requires high, that pollination difficulty is large problem, the invention provides a kind of method of locust tree emasculation and a kind of pollenator, the method is easy, easy to operate, and petal and gynoecium can not be damaged, easy realization pollination, reduces gynoecium generation damage by disease and insect and improves crossbreeding fruit-setting rate.This is consistent with actual result of the test.
(2) method of the present invention easily realizes pollination, improves locust tree Natural seed setting rate; Improve locust tree artificial hybridization success rate of breeding.The method can complete locust tree artificialpollination process, obtains hybrid seed; Locust tree artificial pollination ripening rate can be significantly improved, up to more than 20%; Improve locust tree artificial hybridization success rate of breeding.
Accompanying drawing explanation
Fig. 1 is the structural representation of pollenator of the present invention.
Fig. 2 is the structural representation of rubber of the present invention.
Fig. 3 is locust tree petal expanded view.
Fig. 4 is locust tree flower photo in full bloom.
Fig. 5 is inflorescence and the flower photo of suitable pollination.
Embodiment
Below in conjunction with embodiment, the present invention is described in detail.
Embodiment 1
The invention provides a kind of locust tree artificial emasculation and hybridization controlled pollination method, comprise the following steps:
(1) make pollenator: rubber 4 is cut into long 2cm, the cylinder of diameter 1cm, incite somebody to action a wherein sharpening, for sticky pollen, other end is scabbled, insert a thin wire 3 in centre, be connected with the bottle closure of rubber 2 in vial, thin wire length is about 10cm, sees Fig. 1 and Fig. 2; Wherein vial height 5cm, diameter 2cm, cylindrical rubber bottle stopper.
(2) selected parent sire of hybrid pigs and maternal plant, selects locust tree improved seeds Middle and young forest 6 ~ 20 years raw healthy and strong trees entering generative growth phase as hybridization Parent plant.Early spring suitably prunes elite stand, prunes cella branch off, wipes out tree crown bottom deadwood and thin and delicate branch.Irrigate ground water when trees Germination to Frondescent, use appropriate fertilizer or Chemical Mixed Fertilizer in conjunction with watering, 5kg fertilizer is used in every strain or 1kg trees (fruit tree) compound fertilizer special is advisable.
(3) to gather pollen, spray is gathered after paternal plant enters the early flowering season, indoor are taken by under flower twig clippers with averruncator or secateurs, corolla leading portion is selected obviously to bend the flower that vexil is not yet opened a business in sickle, with scissors corolla first three/mono-in crook cut off half, petal is torn with hand, expose yellow flower pesticide, being cut by flower pesticide or carrying off with hand is placed down on template, after collecting some (about 100) flower, flower pesticide template is wrapped and to be placed in quicklime dry 24 hours, open paper bag, roll flower pesticide gently with round and smooth hard-tipped pen bar and make its loose powder of breaking, pollen is contained in the vial of pollenator and covers tightly bottle cap, be placed on low temperature in refrigerator (0 ~ 4 DEG C) storage,
(4) artificial pollination, maternal plant being selected be suitable for pollinating, (inflorescence that is suitable for pollinating refers to that flower grows to maturation to inflorescence, corolla length no longer increases, corolla leading portion obviously bends in sickle, and vexil is not yet opened a business, now gynoecium is ripe, suitable pollination), left hand gently holds inflorescence, pinches flower with forefinger and thumb, and the right hand takes tip tweezers to be pushed aside by the vexil of flower (Fig. 3 is locust tree petal expanded view), expose stamen, pinch filigree upper end flower pesticide is carried off, repeat to do 2 ~ 3 times, until whole flower pesticide is removed.Then style and column cap is stirred gently with tweezers, column cap is allowed to expose in petal, then the pollenator picking up rapidly adhesion pollen aims at column cap pollination, hit column cap gently and can be stained with pollen, with tweezers, style and column cap are sent back in petal afterwards, allow the style of pollination and column cap reply physical slot, this completes the pollination of 1 flower.Then give the 2nd flower pollination according to the method described above, until all ripe flowers of whole inflorescence have all been awarded, jejune flower is removed, general each inflorescence pollination flower quantity is at 10 ~ 15.Then pollination inflorescence bagging is given, mark female parent and male parent title on sack, pollination flower quantity, pollination date.Hang label, mark female parent and male parent title, date and pollination people to pollination inflorescence sprig simultaneously; The method of giving pollination inflorescence bagging is that the sprig of whole pollination inflorescence growth is all put into sack, and folding sack of clutching is rolled onto on raw old branch last year, sack and branch is fixed to clamp with paper clip or clip, makes sack not by wind rotation or wiped off by wind.Sack template makes, length 27cm, width 17cm, rectangle, one end open.
(5) to pollinate maternal plant management, within 5 ~ 7 days after pollination terminates, remove bagging, gently unfold the branches and leaves of bagging with have gentle hands, add up this inflorescence and to bear fruit flower quantity, record pollination flower quantity.Water to pollination elite stand, spraying pesticide control locust tree aphid (Aphisrobiniae) and Robinia pseudoacacia Seeds chalcid fly (Bruchophagusphilorobiniae), sprayed once every 10 days, continuous administration 2 ~ 3 times in good time.At pollination investigation records in latter about 40 days children pod percentage of fertile fruit.
(6) hybridize ripe pod to gather, 80 ~ 90 days after pollination ripe pods of gathering, immature early than seed in this time pod, being later than this time can increase the mouldy danger lost vigor to seed of pod microbiological contamination.Get out kraft paper bag, ripe pod is cut and is put in paper bag, mark and hybridize Parent.By pod dryness in the sun several days, push pericarp aside and take out seed, seed is put back in paper bag and seals, be placed on indoor shady and cool dry place storage.
Embodiment 2
Conventional locust tree artificial pollination method is, selects maternal plant to be suitable for the inflorescence of pollinating, to flower emasculation, cut the petal of corolla middle and upper part with scissors, expose stamen and gynoecium, then cut stamen, or carry off with hand medicine of deflorating, dip in a little pollen with writing brush and smear column cap, pollinate to column cap.After completing the pollination of whole inflorescence, bagging hangs label.
Experimental example 1
The method of Application Example 1 in 2014 is to the hybrid combination of locust tree different clones composition, implement artificialpollination, as a result, Shandong thorn 57 × emerging thorn 15 hybrid combination ripening rate is 21.28%, the ripening rate of emerging thorn 1 × Shandong thorn 68 is 16.82%, and the ripening rate of thorn 152 × Shanxi, Shandong thorn 4 is 13.05%.In table 1.
Experimental example 2
Within 2013, grope first to adopt the method to the hybrid combination of locust tree different clones composition, implement artificialpollination, as a result, Shandong thorn 57 × emerging thorn 15 hybrid combination ripening rate is 5.91%, the ripening rate of emerging thorn 1 × Shandong thorn 68 is 4.41%, and the ripening rate of thorn 152 × Shanxi, Shandong thorn 4 is 4.32%.In table 1.
Experimental example 3
Within 2012, adopt conventional method (embodiment 2) to the hybrid combination of locust tree different clones composition, implement artificialpollination, result is, Shandong thorn 57 × emerging thorn 15 hybrid combination ripening rate is 1.36%, the ripening rate of emerging thorn 1 × Shandong thorn 68 is 0.47%, and the ripening rate of thorn 152 × Shanxi, Shandong thorn 4 is 0.49%.In table 1.
The different annual Robinia Pseudoacacia Clones hybrid combination artificial pollination ripening rate of table 1
Claims (10)
1. locust tree artificial emasculation and a hybridization controlled pollination method, is characterized in that, comprise the following steps:
(1) pollenator is made: described pollenator comprises contains polliniferous vial, and vial adopts bottle closure of rubber to close, and the bottle closure of rubber in described vial is connected with rubber by wire;
(2) selected parent sire of hybrid pigs and maternal plant;
(3) to gather pollen;
(4) emasculation, pollination: select pollination inflorescence maternal plant, hold inflorescence, pinch flower, the vexil of flower is pushed aside, exposes stamen, pinch filigree upper end and flower pesticide is removed; Stir style and column cap after emasculation, allow column cap expose in petal, then adopt the pollenator of adhesion pollen to aim at column cap pollination, with tweezers, style and column cap are sent back in petal afterwards, allow the style of pollination and column cap recover physical slot;
(5) maternal plant that pollinates manages;
(6) hybridize ripe pod to gather.
2. the method for claim 1, is characterized in that: in step (1), and described rubber is the cylinder shape rubber of a head sharpening other end connection metal silk.
3. the method for claim 1, is characterized in that: in step (2), and the method for selected parent sire of hybrid pigs and maternal plant is: select locust tree improved seeds Middle and young forest 6 ~ 20 years raw healthy and strong trees entering generative growth phase as hybridization Parent plant; Early spring suitably prunes elite stand, prunes cella branch off, wipes out tree crown bottom deadwood and thin and delicate branch; Irrigate ground water when trees Germination to Frondescent, in conjunction with application of organic fertilizers or the Chemical Mixed Fertilizer of watering, 5 ~ 8kg fertilizer or 1 ~ 2kg Chemical Mixed Fertilizer are used in every strain.
4. the method for claim 1, is characterized in that: in step (3), the method for pollen of gathering is: after paternal plant enters the early flowering season, gather spray, selects corolla leading portion obviously to bend the flower that vexil is not yet opened a business in sickle, collects flower pesticide.
5. method as claimed in claim 4, it is characterized in that: concrete method step is: after paternal plant enters the early flowering season, gather spray, indoor are taken by under flower twig clippers with averruncator or secateurs, corolla leading portion is selected obviously to bend the flower that vexil is not yet opened a business in sickle, with scissors corolla first three/mono-in crook cut off half, petal is torn with hand, expose yellow flower pesticide, being cut by flower pesticide or carrying off with hand is placed down on template, after collecting 90-110 flower, flower pesticide template is wrapped and to be placed in quicklime dry 24 hours, open paper bag, roll flower pesticide gently with round and smooth hard-tipped pen bar and make its loose powder of breaking, pollen is contained in the vial of pollenator and covers tightly bottle cap, be placed in the refrigerator of 0 ~ 4 DEG C of low temperature and preserve.
6. the method for claim 1, it is characterized in that: in step (4), concrete method step is: select to be suitable for inflorescence of pollinating on maternal plant, gently hold inflorescence, pinch flower, push aside by the vexil of tip tweezers by flower, expose stamen, pinch filigree upper end flower pesticide is carried off, repeat to do 2 ~ 3 times, until whole flower pesticide is removed; Then style and column cap is stirred gently with tweezers, column cap is allowed to expose in petal, then the pollenator picking up rapidly adhesion pollen aims at column cap pollination, hit column cap gently and can be stained with pollen, with tweezers, style and column cap are sent back in petal afterwards, allow the style of pollination and column cap reply physical slot, after pollination, give pollination inflorescence bagging.
7. method as claimed in claim 6, it is characterized in that: the described inflorescence that is suitable for pollinating is: flower grows to maturation, corolla length no longer increases, corolla leading portion obviously bends in sickle, and vexil is not yet opened a business, now gynoecium is ripe, and suitable pollination, other undesirable flowers are removed from inflorescence.
8. method as claimed in claims 6 or 7, it is characterized in that: the described method to pollination inflorescence bagging is: the sprig of whole pollination inflorescence growth is all put into sack, folding sack of clutching is rolled onto on raw old branch last year, with paper clip or clip, sack and branch are fixed to clamp, make sack not by wind rotate or wiped off by wind, sack template makes.
9. the method for claim 1, it is characterized in that: in step (5), pollination maternal plant management method is: within 5 ~ 7 days after pollination terminates, remove bagging, the branches and leaves of bagging are gently unfolded with have gentle hands, water to pollination elite stand in good time, spraying pesticide pest control, sprayed once every 8 ~ 12 days, continuous administration 2 ~ 3 times.
10. the method for claim 1, is characterized in that: in step (6), and described ripe pod collecting method is: 80 ~ 90 days after pollination ripe pods of gathering; Get out kraft paper bag, ripe pod is cut and is put in paper bag, mark and hybridize Parent; By pod dryness in the sun a couple of days, push pericarp aside and take out seed, seed is put back in paper bag and seals, be placed on indoor shady and cool dry place storage.
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| CN111771659A (en) * | 2020-08-04 | 2020-10-16 | 广西壮族自治区农业科学院 | Method for improving fruiting rate of jasmine flowers and pollination device thereof |
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| CN114402992A (en) * | 2021-12-21 | 2022-04-29 | 黑龙江省林业科学研究所 | Hybrid pollination method for lonicera caerulea |
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| CN111771659A (en) * | 2020-08-04 | 2020-10-16 | 广西壮族自治区农业科学院 | Method for improving fruiting rate of jasmine flowers and pollination device thereof |
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