CN107950387A - A kind of method of agranular cell artificial hybridization under desert climate - Google Patents
A kind of method of agranular cell artificial hybridization under desert climate Download PDFInfo
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- CN107950387A CN107950387A CN201810040684.6A CN201810040684A CN107950387A CN 107950387 A CN107950387 A CN 107950387A CN 201810040684 A CN201810040684 A CN 201810040684A CN 107950387 A CN107950387 A CN 107950387A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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Abstract
The present invention relates to agricultural plantation technology field, the method for the agranular cell artificial hybridization under especially a kind of desert climate, comprises the following steps：S1, using greenhouse gardening；S2, plant in greenhouse cultivation ridge, to be hybridized without kind of skin cucurbita pepo as male parent, using height for self-mating system american pumpkin as female parent, is bred as intermediate self-mating system B1；S3, using intermediate self-mating system B1 as male parent, hybridized using height for self-mating system american pumpkin as female parent, then be returned 34 generations be bred as height for self-mating system B2；S4, using cucurbita pepo height for self-mating system as female parent, using height for self-mating system B2 for male parent or reciprocal cross preparation agranular cell new varieties.The present invention provides a kind of artificial hybridization method of agranular cell, helps to cultivate the agranular cell of high yield, and uses greenhouse gardening, and drip irrigation pipe carries out moisturizing, is adapted to be planted under the conditions of desert climate.
Description
Technical field
The present invention relates to the agranular cell artificial hybridization under agricultural plantation technology field, more particularly to a kind of desert climate
Method.
Background technology
Naked kernel pumpkin (also known as without shell pumpkin) seed benevolence does not plant skin, by comparison can without shelling with common pumpkin
Obtain intact kernel and be beneficial to deep processing, the outlet of Naked kernel pumpkin seed in recent years gradually increases, the growing surface of Naked kernel pumpkin
Product also expands year by year.Naked kernel pumpkin is shown the features such as Comprehensive Traits are good, and melon seeds is big, and yield is high, easy management, deep by vast kind
Plant liking for family.
The kind belong in short climing, stem type, not branch or extremely weak branch, climing light green color have rib ditch and translucent thorn, easily
Adventitious root is produced, internode is short, does not have to during plantation prune or pinch off young shoots.Dark green leaf color, blade are in palmate, there is drastic crack, and blade back vein has
Seta, corolla lobe is long and narrow, expansion, and calyx tube sliver is narrow and short.The prior art lacks its artificial hybridization method, especially lacks desert
Agranular cell artificial hybridization method under weather.
The content of the invention
The purpose of the present invention is to solve shortcoming existing in the prior art, and the nothing under a kind of desert climate proposed
The method of shell cucurbit artificial hybridization.
To achieve these goals, present invention employs following technical solution：
The method for designing the agranular cell artificial hybridization under a kind of desert climate, comprises the following steps：
S1, using greenhouse gardening, cultivation ridge is set in greenhouse, and be recessed 22-24cm, adjacent 2 cultivations ridge spacing 140-
145cm, carries out the planting soil in greenhouse moisturizing, soil moisture maintains 75 ± 5% using drip irrigation pipe；
S2, plant in greenhouse cultivation ridge, using without kind of skin cucurbita pepo as male parent, using height for self-mating system american pumpkin as female parent
Hybridized, be bred as intermediate self-mating system B1；
S3, using intermediate self-mating system B1 as male parent, hybridized using height for self-mating system american pumpkin as female parent, then be returned 3-
In 4 generations, were bred as height for self-mating system B2；
S4, using cucurbita pepo height for self-mating system as female parent, using height for self-mating system B2 for male parent or reciprocal cross preparation agranular cell it is new
Kind.
Preferably, crossbreeding First Year spring is sowed, and summer terminates；Take covering with ground sheeting, ditch kind under film, after late frost
Young plant releases mulch, other management are conventional method.
Preferably, field management is combined before pollination starts and carries out impurity elimination, selection maternal upper 2nd, 3 saves the pollination of position female flower,
Selection every afternoon petal loosens yellowing, the 2nd day open female flower bud and puts on previous ready paper cap, the paper cap folded will with it is female
Spend identical；Pinch petal in lower male parent and loosen yellowing and the 2nd day open male flower bud, the male flower flower bud that will have been pinched the dusk every afternoon time-division
Stand up and given birth to and brought up in the basin for fill shallow water.
Preferably, pollination time selection bloom the same day morning 8 when carry out, before pollination every morning, check maternal field, remove
The female flower of the male flower flower bud of perforated, open male flower and leakage set, when pollination, go out the maternal female paper cap taken, by the male parent of collection
Male flower tears corolla off and exposes stamen, pollen is equably applied on the column cap of female parent, then paper cap is entangled, and makees in paper cap
Mark.
Preferably, it by U.S.'s cucurbita pepo gold rod 1 is basic material that cucurbita pepo height is for self-mating system, using pedigree method, warp
After 7 generations, individual plant selfing selection and breeding form.
The method of agranular cell artificial hybridization under a kind of desert climate proposed by the present invention, beneficial effect are：This hair
It is bright to provide a kind of artificial hybridization method of agranular cell, help to cultivate the agranular cell of high yield, and greenhouse gardening is used,
Drip irrigation pipe carries out moisturizing, is adapted to be planted under the conditions of desert climate.
Embodiment
The technical solution in the embodiment of the present invention is clearly and completely described below, it is clear that described embodiment
Only part of the embodiment of the present invention, instead of all the embodiments.
Embodiment 1
A kind of method of agranular cell artificial hybridization under desert climate, comprises the following steps：
S1, using greenhouse gardening, cultivation ridge is set in greenhouse, and be recessed 22cm, and adjacent 2 cultivations ridge spacing 140cm, uses
Drip irrigation pipe carries out the planting soil in greenhouse moisturizing, and soil moisture maintains 75 ± 5%；
S2, plant in greenhouse cultivation ridge, using without kind of skin cucurbita pepo as male parent, using height for self-mating system american pumpkin as female parent
Hybridized, be bred as intermediate self-mating system B1；
S3, using intermediate self-mating system B1 as male parent, hybridized using height for self-mating system american pumpkin as female parent, then be returned 3
In generation, is bred as height for self-mating system B2；
S4, using cucurbita pepo height for self-mating system as female parent, using height for self-mating system B2 for male parent or reciprocal cross preparation agranular cell it is new
Kind.
Crossbreeding First Year spring is sowed, and summer terminates；Take covering with ground sheeting, ditch kind under film, young plant is put after late frost
Go out mulch, other management are conventional method.
Field management is combined before pollination starts and carries out impurity elimination, maternal upper 2nd, the 3 section position female flower pollination of selection, every afternoon
Selection petal loosens yellowing, the 2nd day open female flower bud puts on previous ready paper cap, and the paper cap folded will coincide with female flower；
Pinch petal in lower male parent and loosen yellowing and the 2nd day open male flower bud the dusk every afternoon time-division, and the male flower flower bud pinched is stood up
Fill and given birth to and brought up in the basin of shallow water.
Pollination time selection bloom the same day morning 8 when carry out, before pollination every morning, check maternal field, remove perforated
The female flower of male flower flower bud, open male flower and leakage set, when pollination, go out the maternal female paper cap taken, and the male parent male flower of collection is torn
Go corolla to expose stamen, pollen is equably applied on the column cap of female parent, then paper cap is entangled, and mark in paper cap.
It by U.S.'s cucurbita pepo gold rod 1 is basic material that cucurbita pepo height is for self-mating system, single by 7 generations using pedigree method
Strain selfed breeding forms.
Embodiment 2
A kind of method of agranular cell artificial hybridization under desert climate, comprises the following steps：
S1, using greenhouse gardening, cultivation ridge is set in greenhouse, and be recessed 23cm, and adjacent 2 cultivations ridge spacing 142cm, uses
Drip irrigation pipe carries out the planting soil in greenhouse moisturizing, and soil moisture maintains 75 ± 5%；
S2, plant in greenhouse cultivation ridge, using without kind of skin cucurbita pepo as male parent, using height for self-mating system american pumpkin as female parent
Hybridized, be bred as intermediate self-mating system B1；
S3, using intermediate self-mating system B1 as male parent, hybridized using height for self-mating system american pumpkin as female parent, then be returned 3
In generation, is bred as height for self-mating system B2；
S4, using cucurbita pepo height for self-mating system as female parent, using height for self-mating system B2 for male parent or reciprocal cross preparation agranular cell it is new
Kind.
Crossbreeding First Year spring is sowed, and summer terminates；Take covering with ground sheeting, ditch kind under film, young plant is put after late frost
Go out mulch, other management are conventional method.
Field management is combined before pollination starts and carries out impurity elimination, maternal upper 2nd, the 3 section position female flower pollination of selection, every afternoon
Selection petal loosens yellowing, the 2nd day open female flower bud puts on previous ready paper cap, and the paper cap folded will coincide with female flower；
Pinch petal in lower male parent and loosen yellowing and the 2nd day open male flower bud the dusk every afternoon time-division, and the male flower flower bud pinched is stood up
Fill and given birth to and brought up in the basin of shallow water.
Pollination time selection bloom the same day morning 8 when carry out, before pollination every morning, check maternal field, remove perforated
The female flower of male flower flower bud, open male flower and leakage set, when pollination, go out the maternal female paper cap taken, and the male parent male flower of collection is torn
Go corolla to expose stamen, pollen is equably applied on the column cap of female parent, then paper cap is entangled, and mark in paper cap.
It by U.S.'s cucurbita pepo gold rod 1 is basic material that cucurbita pepo height is for self-mating system, single by 7 generations using pedigree method
Strain selfed breeding forms.
Embodiment 3
A kind of method of agranular cell artificial hybridization under desert climate, comprises the following steps：
S1, using greenhouse gardening, cultivation ridge is set in greenhouse, and be recessed 23cm, and adjacent 2 cultivations ridge spacing 144cm, uses
Drip irrigation pipe carries out the planting soil in greenhouse moisturizing, and soil moisture maintains 75 ± 5%；
S2, plant in greenhouse cultivation ridge, using without kind of skin cucurbita pepo as male parent, using height for self-mating system american pumpkin as female parent
Hybridized, be bred as intermediate self-mating system B1；
S3, using intermediate self-mating system B1 as male parent, hybridized using height for self-mating system american pumpkin as female parent, then be returned 4
In generation, is bred as height for self-mating system B2；
S4, using cucurbita pepo height for self-mating system as female parent, using height for self-mating system B2 for male parent or reciprocal cross preparation agranular cell it is new
Kind.
Crossbreeding First Year spring is sowed, and summer terminates；Take covering with ground sheeting, ditch kind under film, young plant is put after late frost
Go out mulch, other management are conventional method.
Field management is combined before pollination starts and carries out impurity elimination, maternal upper 2nd, the 3 section position female flower pollination of selection, every afternoon
Selection petal loosens yellowing, the 2nd day open female flower bud puts on previous ready paper cap, and the paper cap folded will coincide with female flower；
Pinch petal in lower male parent and loosen yellowing and the 2nd day open male flower bud the dusk every afternoon time-division, and the male flower flower bud pinched is stood up
Fill and given birth to and brought up in the basin of shallow water.
Pollination time selection bloom the same day morning 8 when carry out, before pollination every morning, check maternal field, remove perforated
The female flower of male flower flower bud, open male flower and leakage set, when pollination, go out the maternal female paper cap taken, and the male parent male flower of collection is torn
Go corolla to expose stamen, pollen is equably applied on the column cap of female parent, then paper cap is entangled, and mark in paper cap.
It by U.S.'s cucurbita pepo gold rod 1 is basic material that cucurbita pepo height is for self-mating system, single by 7 generations using pedigree method
Strain selfed breeding forms.
Embodiment 4
A kind of method of agranular cell artificial hybridization under desert climate, comprises the following steps：
S1, using greenhouse gardening, cultivation ridge is set in greenhouse, and be recessed 24cm, and adjacent 2 cultivations ridge spacing 145cm, uses
Drip irrigation pipe carries out the planting soil in greenhouse moisturizing, and soil moisture maintains 75 ± 5%；
S2, plant in greenhouse cultivation ridge, using without kind of skin cucurbita pepo as male parent, using height for self-mating system american pumpkin as female parent
Hybridized, be bred as intermediate self-mating system B1；
S3, using intermediate self-mating system B1 as male parent, hybridized using height for self-mating system american pumpkin as female parent, then be returned 4
In generation, is bred as height for self-mating system B2；
S4, using cucurbita pepo height for self-mating system as female parent, using height for self-mating system B2 for male parent or reciprocal cross preparation agranular cell it is new
Kind.
Crossbreeding First Year spring is sowed, and summer terminates；Take covering with ground sheeting, ditch kind under film, young plant is put after late frost
Go out mulch, other management are conventional method.
Field management is combined before pollination starts and carries out impurity elimination, maternal upper 2nd, the 3 section position female flower pollination of selection, every afternoon
Selection petal loosens yellowing, the 2nd day open female flower bud puts on previous ready paper cap, and the paper cap folded will coincide with female flower；
Pinch petal in lower male parent and loosen yellowing and the 2nd day open male flower bud the dusk every afternoon time-division, and the male flower flower bud pinched is stood up
Fill and given birth to and brought up in the basin of shallow water.
Pollination time selection bloom the same day morning 8 when carry out, before pollination every morning, check maternal field, remove perforated
The female flower of male flower flower bud, open male flower and leakage set, when pollination, go out the maternal female paper cap taken, and the male parent male flower of collection is torn
Go corolla to expose stamen, pollen is equably applied on the column cap of female parent, then paper cap is entangled, and mark in paper cap.
It by U.S.'s cucurbita pepo gold rod 1 is basic material that cucurbita pepo height is for self-mating system, single by 7 generations using pedigree method
Strain selfed breeding forms.
The foregoing is only a preferred embodiment of the present invention, but protection scope of the present invention be not limited thereto,
Any one skilled in the art the invention discloses technical scope in, technique according to the invention scheme and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (5)
1. the method for the agranular cell artificial hybridization under a kind of desert climate, it is characterised in that comprise the following steps：
S1, using greenhouse gardening, cultivation ridge is set in greenhouse, be recessed 22-24cm, adjacent 2 cultivations ridge spacing 140-145cm,
Moisturizing carries out the planting soil in greenhouse using drip irrigation pipe, soil moisture maintains 75 ± 5%；
S2, plant in greenhouse cultivation ridge, using without kind of skin cucurbita pepo as male parent, using height for self-mating system american pumpkin as maternal progress
Hybridization, is bred as intermediate self-mating system B1；
S3, using intermediate self-mating system B1 as male parent, hybridized using height for self-mating system american pumpkin as female parent, then be returned 3-4 generations
Height is bred as self-mating system B2；
S4, using cucurbita pepo height for self-mating system as female parent, using height for self-mating system B2 for male parent or reciprocal cross preparation agranular cell new varieties.
2. the method for the agranular cell artificial hybridization under a kind of desert climate according to claim 1, it is characterised in that：It is miscellaneous
The sowing of breeding First Year spring is handed over, summer terminates；Take covering with ground sheeting, ditch kind under film, young plant releases mulch after late frost, other
Manage as conventional method.
3. the method for the agranular cell artificial hybridization under a kind of desert climate according to claim 1, it is characterised in that：
Pollination combines field management and carries out impurity elimination, maternal upper 2nd, the 3 section position female flower pollination of selection, selection every afternoon petal pine before starting
Previous ready paper cap is put in dynamic yellowing, the 2nd day open female flower bud, and the paper cap folded will coincide with female flower；Every afternoon is close to
The late time-division pinches petal in lower male parent and loosens yellowing and the 2nd day open male flower bud, and the male flower flower bud pinched is stood up and is filling shallow water
Given birth to and brought up in basin.
4. the method for the agranular cell artificial hybridization under a kind of desert climate according to claim 3, it is characterised in that：Award
Powder selection of time bloom the same day morning 8 when carry out, before pollination every morning, check maternal field, remove the male flower flower bud of perforated, opened
The female flower of the male flower put and leakage set, when pollination, go out the maternal female paper cap taken, and tearing the male parent male flower of collection off corolla exposes
Stamen, pollen is equably applied on the column cap of female parent, then paper cap is entangled, and is marked in paper cap.
5. the method for the agranular cell artificial hybridization under a kind of desert climate according to claim 1, it is characterised in that：West
It by U.S.'s cucurbita pepo gold rod 1 is basic material that cucurbit height is for self-mating system, using pedigree method, by the individual plant selfing selection and breeding of 7 generations
Form.
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| CN201810040684.6A CN107950387A (en) | 2018-01-16 | 2018-01-16 | A kind of method of agranular cell artificial hybridization under desert climate |
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| CN201810040684.6A CN107950387A (en) | 2018-01-16 | 2018-01-16 | A kind of method of agranular cell artificial hybridization under desert climate |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111742839A (en) * | 2020-07-27 | 2020-10-09 | 民勤县全盛永泰农业有限公司 | Artificial hybridization method for shell-free cucurbita pepo in desert climate |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101507414A (en) * | 2009-02-27 | 2009-08-19 | 安徽荃银高科种业股份有限公司 | No-shell India pumpkin parent and variety breeding method |
| CN104303731A (en) * | 2014-08-06 | 2015-01-28 | 山西省农业科学院棉花研究所 | Zucchini breed hybrid seed production method |
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2018
- 2018-01-16 CN CN201810040684.6A patent/CN107950387A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101507414A (en) * | 2009-02-27 | 2009-08-19 | 安徽荃银高科种业股份有限公司 | No-shell India pumpkin parent and variety breeding method |
| CN104303731A (en) * | 2014-08-06 | 2015-01-28 | 山西省农业科学院棉花研究所 | Zucchini breed hybrid seed production method |
Non-Patent Citations (3)
| Title |
|---|
| 周旭东: ""金丰宝"品种高产栽培技术", 《新疆农业科技》 * |
| 张艳红: "裸仁南瓜绿农1号的选育及高产栽培技术", 《作物杂志》 * |
| 霍志军等: "《种子生产与管理》", 31 December 2009 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111742839A (en) * | 2020-07-27 | 2020-10-09 | 民勤县全盛永泰农业有限公司 | Artificial hybridization method for shell-free cucurbita pepo in desert climate |
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