CN107295967A - A kind of gumbo combined hybrid method - Google Patents
A kind of gumbo combined hybrid method Download PDFInfo
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- CN107295967A CN107295967A CN201710555879.XA CN201710555879A CN107295967A CN 107295967 A CN107295967 A CN 107295967A CN 201710555879 A CN201710555879 A CN 201710555879A CN 107295967 A CN107295967 A CN 107295967A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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Abstract
The invention discloses a kind of gumbo combined hybrid method, including：(1) breeding base selection；(2) soak seed；(3) soil prepares；(4) sow；(5) father, maternal field arrangement；(6) roguing；(7) maternal plant arranges and father, maternal isolation；(8) stamen is removed；(9) pollinate；(10) invalid colored pod is removed；(11) pluck.This hybridizing method provides the cross breeding method of a set of arrangement including maternal plant, maternal emasculation, artificial pollination, fruit management and seed collection for crossbreeding cultivation and father, maternal combination pairing.
Description
Technical field
The present invention relates to vegetables crossbreeding technology field, more particularly to a kind of gumbo combined hybrid method.
Background technology
Abelmoschus cross pollinated plant, with obvious hybrid vigour.Conventional cross-breeding, also referred to as Combination breeding.Pass through
Artificial hybridization, the merit being scattered on different parents is combined in hybrid, is carried out be commissioned to train more to its offspring and is educated selection, obtains
Obtain genotype homozygosis or the new varieties close to homozygosis.But the gumbo crossbreeding technology of China is lack of standardization, there are some deficiencies
Place：1) germ plasm resource is deficient, and hereditary basis is narrow, and the fine quality of real suitable southern China climate and soil property is less；2) gumbo is miscellaneous
Friendship method also imperfection, crossover operation is present when shortcoming for example removes stamen due to selecting instrument improper or operating wound lack of standardization
And column cap, cause emasculation unsuccessfully to reduce operating efficiency；3) it is not good to being carried out between maternal flower and the external world after emasculation
Isolation, causes extraneous factor to be damaged to style or in advance by other pollen contaminations, causes hybrid purity and be hybridized to pod
Rate is reduced.
The content of the invention
The technical problem to be solved in the present invention is：A kind of gumbo combined hybrid method is provided, keep cenospecies purity and
Good strains of seeds, the industrialization for gumbo cenospecies industry provides technical support.
A kind of gumbo combined hybrid method, comprises the following steps：
(1) breeding base selection：Select east, west, the face of south three without veil, ground is solid, flat, hidding and sunny side, soil
Loose fertile, salt-free and other pollutions, the clean ground of water quality；
(2) soak seed：Declined naturally in air by water temperature with 40~55 DEG C of water seed soakings, 25~28 DEG C of germination temperature, revealed
Sowed after Bai Jieqi；
(3) soil prepares：30~40cm of deep ploughing is carried out to soil, while applying organic fertilizer, hard thing is removed；
(4) sow：Male parent, maternal combined respectively from Rodococcus sp J-5 with okra are cultivated, and there are seedling 2 leaves wholeheartedly to move
Plant, 50~80cm of line-spacing, 80~100cm of spacing in the rows；
(5) father, maternal field arrangement：Father, maternal ratio press 1:2 configurations, a line male parent kind is between two rows are maternal；
(6) roguing：In seedling stage and before starting hybridization, according to father, maternal typicalness, strictly pull out in seed farm
Hybrid strain, weak plant, variant and diseased plant；
(7) maternal plant arranges and father, maternal isolation：In emasculation the previous day, opened all on pistillate parent
Flower and the fruit tied extract, while to father that is open and will opening, it is maternal carry out bagging, and in emasculation when the sky
Noon extracts the flower of the same day maternal all new openings；
(8) stamen is removed：Full with calyx on maternal plant, micro- yellow corolla or the petal of red corolla of exposing is emasculation
Object, emasculation work is carried out after fine at 2 points in afternoon, both hands and emasculation instrument tweezers are carried out with 75% alcohol
Sterilization, then breaks calyx petal into two with one's hands with hand and stamen is removed with tip 15cm or elbow 15cm tweezers, with saturating after emasculation
The template of light carries out bagging；Emasculation is thorough, not remains of pesticide on style, must not damage ovary and style, keep outside ovary
Tunica albuginea and bract it is complete；
(9) pollinate：The next morning 8 after emasculation step:30~9:30 are pollinated, and same product are gently stained with hairbrush
Kind plurality of flowers powder be collected in sulfuric acid paper bag, and be stained with same branch hairbrush pollen in being uniformly distributed it on the column cap of emasculation
On style, pollination amount is sufficient uniform, it is to avoid leakage pollination, puts the sulfuric acid paper bag of printing opacity again afterwards, and mark is listed；
(10) invalid colored pod is removed：After pollination terminates, all invalid colored pods in female parent strain, and thereafter 12 are thoroughly removed
Checked once every 3 days in it, strictly remove the flower pod newly gone out；
(11) pluck：When the whole browning crackings of okra, when seed color is deeper, plucked, harvesting circle slips full fruit
It is real, the fruit of harvesting is placed on exposure under the sun and causes fruit natural cracking, slag is carried out with machinery, separation is planted, the gumbo of collection
Seed part several times in the morning 8~11 points dried to suitable storing aqueous rate condition, be placed in 0 after progress close drying afterwards
DEG C refrigeration.
The indexs such as yield, resistance to improve gumbo, make it contain higher Vc, polysaccharide, protein and anthocyanidin, can lead to
Cross Rodococcus sp J-5 with okra to be hybridized, be specially：The female parent is Red Star Rodococcus sp J-5, and male parent is that spring okra or green star are yellow
Gumbo；Or the female parent is green star okra, male parent is the precious Rodococcus sp J-5 of China or Red Star Rodococcus sp J-5.
Wherein, Pollen Activity measure is carried out before step (9) pollination.
It is strict to remove selfing pod to maternal field by hauling type inspection is carried out by pod before step (11) harvesting.
Breeding base of the present invention field is early, evening respectively pours a water, and two weeks applies disposable fertilizer.
The present invention has the advantages that：
1) stamen is removed with the tweezers of appropriate length, column cap will not be injured, it is to avoid the mucus of bud pulls apart thin and delicate style,
Improve emasculation success rate and operating efficiency；
2) father, maternal isolation are carried out to father, the maternal good sulfuric acid paper bag of light transmittance that puts before and after emasculation, can effectively prevented non-
Paternal pollen is fallen within maternal column cap, reduces the injury of outer bound pair style, is improved hybrid purity and is hybridized to pod rate, at utmost
The upper vigor for ensureing pollen, makes fertilization fully, seed plumpness is improved, and improves bud rate；
3) pollination is carried out using pollen mixture and improves pollination efficiency, retain male parent flower, paternal plant when collecting pollen
Green bean can be harvested, increases economic benefit；
4) seed oil content is high, can be used for oil expression, forges into the okra vegetable oil of a new nutrient health, creates
Economic value；
5) it is that crossbreeding cultivation and father, maternal combination pairing provide a set of arrangement including maternal plant, female parent
Hero, artificial pollination, fruit management and seed collection cross breeding method.
Embodiment
In the examples where no specific technique or condition is specified, according to the technology or condition described by document in the art
(write such as with reference to J. Pehanorm Brookers, what Huang Peitang etc. was translated《Molecular Cloning:A Laboratory guide》, the third edition, Science Press) or
Person is carried out according to product description.Agents useful for same or the unreceipted production firm person of instrument, being can be by the normal of acquisition purchased in market
Advise product.
Embodiment 1
A kind of gumbo combined hybrid method, comprises the following steps：
(1) breeding base selection：Select east, west, the face of south three without veil, ground is solid, flat, hidding and sunny side, soil
Loose fertile, salt-free and other pollutions, the clean ground of water quality；
(2) soak seed：Declined naturally in air by water temperature with 40~55 DEG C of water seed soakings, 25~28 DEG C of germination temperature, revealed
Sowed after Bai Jieqi；
(3) soil prepares：30~40cm of deep ploughing is carried out to soil, while applying organic fertilizer, hard thing is removed；
(4) sow：Male parent selects spring okra, and female parent is cultivated from the combination of Red Star Rodococcus sp J-5, and seedling has 2 leaves one
The heart is transplanted, line-spacing 50cm, spacing in the rows 80cm；
(5) father, maternal field arrangement：Father, maternal ratio press 1:2 configurations, a line male parent kind is between two rows are maternal；
(6) roguing：In seedling stage and before starting hybridization, according to father, maternal typicalness, strictly pull out in seed farm
Hybrid strain, weak plant, variant and diseased plant；
(7) maternal plant arranges and father, maternal isolation：In emasculation the previous day, opened all on pistillate parent
Flower and the fruit tied extract, while to father that is open and will opening, it is maternal carry out bagging, and in emasculation when the sky
Noon extracts the flower of the same day maternal all new openings；
(8) stamen is removed：Full with calyx on maternal plant, micro- yellow corolla or the petal of red corolla of exposing is emasculation
Object, emasculation work is carried out after fine at 2 points in afternoon, both hands and emasculation instrument tweezers are carried out with 75% alcohol
Sterilization, then breaks calyx petal into two with one's hands with hand and stamen is removed with tip 15cm or elbow 15cm tweezers, with saturating after emasculation
The template of light carries out bagging；Emasculation is thorough, not remains of pesticide on style, must not damage ovary and style, keep outside ovary
Tunica albuginea and bract it is complete；
(9) pollinate：The next morning 8 after emasculation step:30~9:30 are pollinated, and first carry out Pollen Activity survey
It is fixed, determine after passing, the plurality of flowers powder that same kind is gently stained with hairbrush is collected in sulfuric acid paper bag, and is stained with same branch hairbrush
Pollen on the column cap of emasculation in making it be evenly distributed on style, and pollination amount is sufficient uniform, it is to avoid leakage pollination, puts again afterwards
The sulfuric acid paper bag of printing opacity, mark is listed；
(10) invalid colored pod is removed：After pollination terminates, all invalid colored pods in female parent strain, and thereafter 12 are thoroughly removed
Checked once every 3 days in it, strictly remove the flower pod newly gone out；
(11) pluck：When the whole browning crackings of okra, when seed color is deeper, plucked, to maternal field before harvesting
Hauling type inspection is carried out by pod by, strict to remove selfing pod, harvesting circle slips full fruit, the fruit of harvesting is placed under the sun
Exposure causes fruit natural cracking, and slag is carried out with machinery, plants and separates, in the morning 8~11 points several times of the gumbo seed part of collection
Dried to suitable storing aqueous rate condition, 0 DEG C of refrigeration is placed in after carrying out close drying afterwards.
Breeding base field is early, evening respectively pours a water, and two weeks applies disposable fertilizer.
Embodiment 2
A kind of gumbo combined hybrid method, comprises the following steps：
(1) breeding base selection：Select east, west, the face of south three without veil, ground is solid, flat, hidding and sunny side, soil
Loose fertile, salt-free and other pollutions, the clean ground of water quality；
(2) soak seed：Declined naturally in air by water temperature with 40~55 DEG C of water seed soakings, 25~28 DEG C of germination temperature, revealed
Sowed after Bai Jieqi；
(3) soil prepares：30~40cm of deep ploughing is carried out to soil, while applying organic fertilizer, hard thing is removed；
(4) sow：Male parent selects green star okra, and female parent is cultivated from the combination of Red Star Rodococcus sp J-5, and seedling has 2 leaves one
The heart is transplanted, line-spacing 55cm, spacing in the rows 80cm；
(5) father, maternal field arrangement：Father, maternal ratio press 1:2 configurations, a line male parent kind is between two rows are maternal；
(6) roguing：In seedling stage and before starting hybridization, according to father, maternal typicalness, strictly pull out in seed farm
Hybrid strain, weak plant, variant and diseased plant；
(7) maternal plant arranges and father, maternal isolation：In emasculation the previous day, opened all on pistillate parent
Flower and the fruit tied extract, while to father that is open and will opening, it is maternal carry out bagging, and in emasculation when the sky
Noon extracts the flower of the same day maternal all new openings；
(8) stamen is removed：Full with calyx on maternal plant, micro- yellow corolla or the petal of red corolla of exposing is emasculation
Object, emasculation work is carried out after fine at 2 points in afternoon, both hands and emasculation instrument tweezers are carried out with 75% alcohol
Sterilization, then breaks calyx petal into two with one's hands with hand and stamen is removed with tip 15cm or elbow 15cm tweezers, with saturating after emasculation
The template of light carries out bagging；Emasculation is thorough, not remains of pesticide on style, must not damage ovary and style, keep outside ovary
Tunica albuginea and bract it is complete；
(9) pollinate：The next morning 8 after emasculation step:30~9:30 are pollinated, and first carry out Pollen Activity survey
It is fixed, determine after passing, the plurality of flowers powder that same kind is gently stained with hairbrush is collected in sulfuric acid paper bag, and is stained with same branch hairbrush
Pollen on the column cap of emasculation in making it be evenly distributed on style, and pollination amount is sufficient uniform, it is to avoid leakage pollination, puts again afterwards
The sulfuric acid paper bag of printing opacity, mark is listed；
(10) invalid colored pod is removed：After pollination terminates, all invalid colored pods in female parent strain, and thereafter 12 are thoroughly removed
Checked once every 3 days in it, strictly remove the flower pod newly gone out；
(11) pluck：When the whole browning crackings of okra, when seed color is deeper, plucked, to maternal field before harvesting
Hauling type inspection is carried out by pod by, strict to remove selfing pod, harvesting circle slips full fruit, the fruit of harvesting is placed under the sun
Exposure causes fruit natural cracking, and slag is carried out with machinery, plants and separates, in the morning 8~11 points several times of the gumbo seed part of collection
Dried to suitable storing aqueous rate condition, 0 DEG C of refrigeration is placed in after carrying out close drying afterwards.
Breeding base field is early, evening respectively pours a water, and two weeks applies disposable fertilizer.
Embodiment 3
A kind of gumbo combined hybrid method, comprises the following steps：
(1) breeding base selection：Select east, west, the face of south three without veil, ground is solid, flat, hidding and sunny side, soil
Loose fertile, salt-free and other pollutions, the clean ground of water quality；
(2) soak seed：Declined naturally in air by water temperature with 40~55 DEG C of water seed soakings, 25~28 DEG C of germination temperature, revealed
Sowed after Bai Jieqi；
(3) soil prepares：30~40cm of deep ploughing is carried out to soil, while applying organic fertilizer, hard thing is removed；
(4) sow：Male parent is cultivated from the precious Rodococcus sp J-5 of China, female parent from the combination of green star okra, and seedling has 2 leaves one
The heart is transplanted, line-spacing 60cm, spacing in the rows 90cm；
(5) father, maternal field arrangement：Father, maternal ratio press 1:2 configurations, a line male parent kind is between two rows are maternal；
(6) roguing：In seedling stage and before starting hybridization, according to father, maternal typicalness, strictly pull out in seed farm
Hybrid strain, weak plant, variant and diseased plant；
(7) maternal plant arranges and father, maternal isolation：In emasculation the previous day, opened all on pistillate parent
Flower and the fruit tied extract, while to father that is open and will opening, it is maternal carry out bagging, and in emasculation when the sky
Noon extracts the flower of the same day maternal all new openings；
(8) stamen is removed：Full with calyx on maternal plant, micro- yellow corolla or the petal of red corolla of exposing is emasculation
Object, emasculation work is carried out after fine at 2 points in afternoon, both hands and emasculation instrument tweezers are carried out with 75% alcohol
Sterilization, then breaks calyx petal into two with one's hands with hand and stamen is removed with tip 15cm or elbow 15cm tweezers, with saturating after emasculation
The template of light carries out bagging；Emasculation is thorough, not remains of pesticide on style, must not damage ovary and style, keep outside ovary
Tunica albuginea and bract it is complete；
(9) pollinate：The next morning 8 after emasculation step:30~9:30 are pollinated, and first carry out Pollen Activity survey
It is fixed, determine after passing, the plurality of flowers powder that same kind is gently stained with hairbrush is collected in sulfuric acid paper bag, and is stained with same branch hairbrush
Pollen on the column cap of emasculation in making it be evenly distributed on style, and pollination amount is sufficient uniform, it is to avoid leakage pollination, puts again afterwards
The sulfuric acid paper bag of printing opacity, mark is listed；
(10) invalid colored pod is removed：After pollination terminates, all invalid colored pods in female parent strain, and thereafter 12 are thoroughly removed
Checked once every 3 days in it, strictly remove the flower pod newly gone out；
(11) pluck：When the whole browning crackings of okra, when seed color is deeper, plucked, to maternal field before harvesting
Hauling type inspection is carried out by pod by, strict to remove selfing pod, harvesting circle slips full fruit, the fruit of harvesting is placed under the sun
Exposure causes fruit natural cracking, and slag is carried out with machinery, plants and separates, in the morning 8~11 points several times of the gumbo seed part of collection
Dried to suitable storing aqueous rate condition, 0 DEG C of refrigeration is placed in after carrying out close drying afterwards.
Breeding base field is early, evening respectively pours a water, and two weeks applies disposable fertilizer.
Embodiment 4
A kind of gumbo combined hybrid method, comprises the following steps：
(1) breeding base selection：Select east, west, the face of south three without veil, ground is solid, flat, hidding and sunny side, soil
Loose fertile, salt-free and other pollutions, the clean ground of water quality；
(2) soak seed：Declined naturally in air by water temperature with 40~55 DEG C of water seed soakings, 25~28 DEG C of germination temperature, revealed
Sowed after Bai Jieqi；
(3) soil prepares：30~40cm of deep ploughing is carried out to soil, while applying organic fertilizer, hard thing is removed；
(4) sow：Male parent selects Red Star Rodococcus sp J-5, and female parent is cultivated from the combination of green star okra, and seedling has 2 leaves one
The heart is transplanted, line-spacing 70cm, spacing in the rows 100cm；
(5) father, maternal field arrangement：Father, maternal ratio press 1:2 configurations, a line male parent kind is between two rows are maternal；
(6) roguing：In seedling stage and before starting hybridization, according to father, maternal typicalness, strictly pull out in seed farm
Hybrid strain, weak plant, variant and diseased plant；
(7) maternal plant arranges and father, maternal isolation：In emasculation the previous day, opened all on pistillate parent
Flower and the fruit tied extract, while to father that is open and will opening, it is maternal carry out bagging, and in emasculation when the sky
Noon extracts the flower of the same day maternal all new openings；
(8) stamen is removed：Full with calyx on maternal plant, micro- yellow corolla or the petal of red corolla of exposing is emasculation
Object, emasculation work is carried out after fine at 2 points in afternoon, both hands and emasculation instrument tweezers are carried out with 75% alcohol
Sterilization, then breaks calyx petal into two with one's hands with hand and stamen is removed with tip 15cm or elbow 15cm tweezers, with saturating after emasculation
The template of light carries out bagging；Emasculation is thorough, not remains of pesticide on style, must not damage ovary and style, keep outside ovary
Tunica albuginea and bract it is complete；
(9) pollinate：The next morning 8 after emasculation step:30~9:30 are pollinated, and first carry out Pollen Activity survey
It is fixed, determine after passing, the plurality of flowers powder that same kind is gently stained with hairbrush is collected in sulfuric acid paper bag, and is stained with same branch hairbrush
Pollen on the column cap of emasculation in making it be evenly distributed on style, and pollination amount is sufficient uniform, it is to avoid leakage pollination, puts again afterwards
The sulfuric acid paper bag of printing opacity, mark is listed；
(10) invalid colored pod is removed：After pollination terminates, all invalid colored pods in female parent strain, and thereafter 12 are thoroughly removed
Checked once every 3 days in it, strictly remove the flower pod newly gone out；
(11) pluck：When the whole browning crackings of okra, when seed color is deeper, plucked, to maternal field before harvesting
Hauling type inspection is carried out by pod by, strict to remove selfing pod, harvesting circle slips full fruit, the fruit of harvesting is placed under the sun
Exposure causes fruit natural cracking, and slag is carried out with machinery, plants and separates, in the morning 8~11 points several times of the gumbo seed part of collection
Dried to suitable storing aqueous rate condition, 0 DEG C of refrigeration is placed in after carrying out close drying afterwards.
Breeding base field is early, evening respectively pours a water, and two weeks applies disposable fertilizer.
The better embodiment to the present invention is illustrated above, but the invention is not limited to the implementation
Example, those skilled in the art can also make a variety of equivalent modifications or replace on the premise of without prejudice to spirit of the invention
Change, these equivalent modifications or replacement are all contained in the application claim limited range.
Claims (6)
1. a kind of gumbo combined hybrid method, it is characterised in that comprise the following steps：
(1) breeding base selection：
Select east, west, the face of south three without veil, ground is solid, flat, hidding and sunny side, and loosing soil is fertile, salt-free and other are dirty
Dye, the clean ground of water quality；
(2) soak seed：
Declined naturally in air by water temperature with 40~55 DEG C of water seed soakings, 25~28 DEG C of germination temperature, sowed after the solar term that show money or valuables one carries unintentionally；
(3) soil prepares：
30~40cm of deep ploughing is carried out to soil, while applying organic fertilizer, hard thing is removed；
(4) sow：
Male parent, maternal combined respectively from Rodococcus sp J-5 with okra are cultivated, and there are seedling 2 leaves wholeheartedly to transplant, and line-spacing 50~
80cm, 80~100cm of spacing in the rows；
(5) father, maternal field arrangement：
Father, maternal ratio press 1:2 configurations, a line male parent kind is between two rows are maternal；
(6) roguing：
In seedling stage and before starting hybridization, according to father, maternal typicalness, hybrid strain in seed farm, weak plant, variant are strictly pulled out
And diseased plant；
(7) maternal plant arranges and father, maternal isolation：
In emasculation the previous day, all flowers opened on pistillate parent and the fruit tied are extractd, at the same to open and
The father that will open, it is maternal carry out bagging, and on the day of that morning of emasculation is extractd maternal all new openings flower；
(8) stamen is removed：
Full with calyx on maternal plant, micro- yellow corolla or the petal of red corolla of exposing is emasculation object, fine
At 2 points in afternoon after carry out emasculation work, both hands and emasculation instrument tweezers are carried out disinfection with 75% alcohol, hand handle is then used
Calyx petal is broken into two with one's hands and stamen is removed with tip 15cm or elbow 15cm tweezers, is covered after emasculation with the template of printing opacity
Bag；Emasculation is thorough, not remains of pesticide on style, must not damage ovary and style, keeps the complete of the outer tunica albuginea of ovary and bract；
(9) pollinate：
The next morning 8 after emasculation step:30~9:30 are pollinated, and the plurality of flowers powder of same kind is gently stained with hairbrush
It is collected in sulfuric acid paper bag, and pollen is stained with making it be evenly distributed on style on the column cap of emasculation with same branch hairbrush, awards
Powder amount is sufficient uniform, it is to avoid leakage pollination, puts the sulfuric acid paper bag of printing opacity again afterwards, and mark is listed；
(10) invalid colored pod is removed：
After pollination terminates, all invalid colored pods in female parent strain are thoroughly removed, and were checked once every 3 days in 12 days thereafter,
Strictly remove the flower pod newly gone out；
(11) pluck：
When the whole browning crackings of okra, when seed color is deeper, plucked, harvesting circle slips full fruit, by the fruit of harvesting
Exposure under the sun is placed in fact and causes fruit natural cracking, slag is carried out with machinery, separation is planted, and the gumbo seed part of collection is existed several times
Morning 8~11 point are dried to suitable storing aqueous rate condition, and 0 DEG C of refrigeration is placed in after carrying out close drying afterwards.
2. gumbo combined hybrid method according to claim 1, it is characterised in that step (4) is described maternal for the Red Star red autumn
Certain herbaceous plants with big flowers, male parent is spring okra or green star okra.
3. gumbo combined hybrid method according to claim 1, it is characterised in that step (4) is described maternal for the green star yellow autumn
Certain herbaceous plants with big flowers, male parent is the precious Rodococcus sp J-5 of China or Red Star Rodococcus sp J-5.
4. gumbo combined hybrid method according to claim 1, it is characterised in that carry out Pollen Activity before step (9) pollination
Determine.
5. gumbo combined hybrid method according to claim 1, it is characterised in that before step (11) harvesting, to maternal field by
Hauling type inspection is carried out by pod, it is strict to remove selfing pod.
6. gumbo combined hybrid method according to claim 1, it is characterised in that breeding base field is early, evening respectively pours one
Secondary water, two weeks applies disposable fertilizer.
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| CN112493120A (en) * | 2020-12-21 | 2021-03-16 | 江苏高航农业科技有限公司 | Embryo rescue method for distant hybridization of okra cultivars and wild okra |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112493120A (en) * | 2020-12-21 | 2021-03-16 | 江苏高航农业科技有限公司 | Embryo rescue method for distant hybridization of okra cultivars and wild okra |
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