CN104255438A - Breeding method of forage grass soybean - Google Patents
Breeding method of forage grass soybean Download PDFInfo
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- CN104255438A CN104255438A CN201410527254.9A CN201410527254A CN104255438A CN 104255438 A CN104255438 A CN 104255438A CN 201410527254 A CN201410527254 A CN 201410527254A CN 104255438 A CN104255438 A CN 104255438A
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Abstract
The invention relates to the technical field of soybean variety breeding, and particularly relates to a breeding method of a forage grass type bean. The breeding method comprises the following steps: taking a bean 38 as a female parent and taking a wild soybean DYKSW-3 as a male parent to carry out hybridization to obtain a hybrid first generation seed, carrying out multi-generation selfing purification according to a pedigree method, and selecting upright single plants with more branches as seeds during the selfing to obtain the forage grass type bean. The bred variety is capable of thoroughly changing the growth characteristic of male parent winding, the forage grass type bean grows uprightly, many branches begin from the basal part of the stem; the leaf number of the male parent is large, and the pods start from the basal part of the stem and are dense and compact, so that the soybean is high in quality and medium in yield and has relatively high feeding value.
Description
technical field
The present invention relates to soybean varieties breeding technique field, particularly the selection of a kind of forage grass type soybean.
background technology
Leguminous forage because of its be of high nutritive value, good palatability and become the high quality grass of Livestock, it is the important component of Livestock daily ration composition, and China's high-quality legume forage kind is poor, the suitable leguminous forage kind of planting in East China is very few especially, therefore, cultivation product are of fine quality, output is high, adaptable leguminous forage kind, become the task of top priority.
Annual wild soybean extensively distributes in the Delta Region of The Yellow River in Shandong, has stem softness, blade amount is large, nutritious, green forage yield is high, the feature such as good palatability, and feeding value is high, disease and insect resistance and strong adaptability.But its serious winding growth characteristics, make it under unicast conditions, bottom blade is often withered and yellow too early because sheltering from heat or light, and causes the reduction of herbage quality and output, is also unfavorable for mechanized harvest, be difficult to large-scale production.Therefore, a kind of upright forage grass type soybean of seed selection, as herbage, has important production practical significance.
summary of the invention
In order to solve the problem that wild soybean causes quality and yield low because of serious winding characteristic, the invention provides one and utilize the cultivated soybean vertical property, carry out selection cross with annual wild soybean, cultivate vertical growth, herbage quality is excellent and the selection of the forage grass type soybean of the forage grass type new soybean varieties that output is higher.
The present invention is achieved by the following measures:
The selection of a kind of forage grass type soybean comprises the following steps:
38 are handed over to be maternal with beans, wild soybean DYKSW-3 is male parent, carry out artificial pollination, obtain first-filial generation seed, selfing after plantation, the independent sowing of every strain obtains second filial generation seed, plantation, from second filial generation plant, screen upright and that branch amount is many individual plant reserve seed for planting, and carry out inbreeding of more generation purifying, to obtain final product.
Described selection, preferably from second filial generation, filters out upright and stem branch amount and reserves seed for planting more than the individual plant of more than 10 from segregative generation.
Described selection, preferably with the yield and quality of plant nutrition body for breeding goal, from the strain after inbreeding of more generation purifying, select the strain that output is high, product are of fine quality to reserve seed for planting.
Described selection, preferred screening individual plant forage yield is the highest, crude protein content is reserved seed for planting higher than the strain of 18%.
Described selection, preferably includes following steps:
(1) plant first-filial generation seed, selfing, the independent sowing of every strain, obtains second filial generation seed;
(2) by individual plant branch plantation second filial generation seed, in the plant of trait segregation, carry out mark and independent sowing to upright individual plant, and carry out the observation of branch amount between individual plant and compare, choosing upright and stem branch amount higher than the individual plant of 10 is hybridization three generations seed;
(3) plantation hybridization three generations seed, continuing to choose upright and stem branch amount higher than the individual plant of 10 is hybridization four generation seed;
(4) plant hybridization four generation seed, carry out the test of full-bloom stage forage yield and nutrient content, screening individual plant forage yield is the highest, and crude protein content, higher than the strain of 18%, is gathered in the crops hybridization five generation seed, to be obtained final product.
Described selection, the maternal interlacing plantation of preferred male parent.
Described selection, the mode that preferred artificial pollination adopts " late emasculation, early pollination ", the bud that dew is purple selected by maternal the cultivated soybean, and 4-6 point emasculation in the afternoon, with the column cap that nearest blade protection is exposed, pollinated after morning next day, 7-9 point got pollen immediately.
Beneficial effect of the present invention:
1, the growth characteristics that soybean No. 2 revolutionizes the winding of its male parent are raised in Shandong, vertical growth, and branch from basal part of stem, branch quantity is many, have again the characteristic that male parent leaf amount is large concurrently, and bear pods from basal part of stem, pod is close and real, is an annual feeding soybean varieties that quality is high, output is medium, has higher feeding value.Varietal yield test shows, and full-bloom stage cradles green forage yield 32154 kg/hm
2, hay yield 9887.4 kg/hm
2;
2, because soybean No. 2 cauline leaf grow prolificallies are raised in Shandong, leaf amount is large, and nutrition content is high, full-bloom stage plant, and in dry matter, crude protein content can reach 19.7 %, ADF content 36.1 %, and NDF content 45.6 %, and good palatability are the high quality grass of Herbivores.
Embodiment
For a better understanding of the present invention, further illustrate below in conjunction with specific embodiment.
embodiment 1:
2006: the soybean varieties of plantation beans friendship 38, No. one, shore duty beans, holy rich middle beans 31, fodder food beans 4 Different ripening stages and 8 different ecological type wild soybeans (WFLSW-2, WFQSW-2, WFJPW-1, ZBHPW-1, DYKSW-3, QDJSW-3, DYKYW-4, BZZYW-4), adopt artificial pollination mode, the sowing time sees the following form.
Results of hybridization sees the following form.
Planted by the first-filial generation seed gathered in the crops in upper table, results second filial generation seed, 250 to 350, plantation second filial generation seed, observes proterties, the results are shown in following table.
Because beans hand over 38 be maternal, DYKSW-3 is that the filial generation of male parent occurs the upright and individual plant that branch amount is many, so select this to be combined into capable further selfing purifying.
The first-filial generation seed F1 of " beans hand over the 38(people No. 2 x to defend 4) for maternal, wild soybean DYKSW-3 is male parent ".
2007: plantation first-filial generation seed F1,8 combinations receive the strain of true hybrid 10, the independent sowing of every strain altogether, obtain second filial generation seed F2.
2008: by individual plant branch plantation second filial generation seed F2.In the plant of trait segregation, carry out mark and independent sowing, and carry out the observation of branch amount between individual plant and compare upright individual plant, choosing upright and stem branch amount higher than the individual plant of 10 is hybridization three generations seed F3.
2009: F3 is for seed in plantation, continuing to choose upright and stem branch amount higher than the individual plant of 10 was hybridization four generation seed F4.
2010: plant F4 for seed, carry out the test of full-bloom stage forage yield and nutrient content, screening individual plant forage yield was the highest, and crude protein content, higher than the strain of 18%, gathers in the crops hybridization five generation seed F5, enters district's examination and production test.
2011-2013 years in Jinan, Tai'an, Wudi, the Tai Er village and the Zhangqiu strain to screening carry out varietal yield test, regional test and production test, obtains the growth characteristics of multiple years, output situation and attributional analysis data.
2013, on test data analyzer basis for many years, specify its stability and grass yield, declare as soybean No. 2 feeding soybean varieties are raised in Shandong.
Parent is all on the books in following documents and materials:
1. male parent 8 different ecological type wild soybeans (WFLSW-2, WFQSW-2, WFJPW-1, ZBHPW-1, DYKSW-3, QDJSW-3, DYKYW-4, BZZYW-4) are disclosed report in the beautiful thesis for the doctorate of Zhai Gui, " the forage grass productivity of annual wild soybean and culture and utility technical research ", Zhai Guiyu, Agricultural University Of Nanjing, 2007, thesis for the doctorate.
2. maternal beans hand over the 38(people No. 2 x to defend 4) be in the news in the following references: " farmers' staff officer " 1994.4 phase 17-18 page, summer soybean new varieties " peaceful No. 4 of mountain " Hu Xulian, Gai Cuixiang, Chen Yilan.
3. maternal No. one, shore duty beans (day general election × " shore 89036 ") are in the news in the following references: No. one, " soybean circular " 2004 the 4th phase 10 pages of new soybean varieties shore duty beans and the red Zhang Junsheng of culture technique Xu Qing Xin Lirui.
Shandong is raised under soybean No. 2 is suitable for warm and moist weather conditions and is grown, and needs sufficient sunlight, and plant needs water, fertilizer plentiful in vegetative period, and require tight to soil, but acid ground is unfavorable for Nodule Growth, breeding time is about 130 d.
The growth characteristics that soybean No. 2 revolutionizes the winding of its male parent are raised in Shandong, vertical growth, and branch from basal part of stem, branch quantity is many, have again the characteristic that male parent leaf amount is large concurrently, and bear pods from basal part of stem, pod is close and real, is an annual feeding soybean varieties that quality is high, output is medium, has higher feeding value.Varietal yield test shows, and full-bloom stage cradles green forage yield 32154 kg/hm
2, hay yield 9887.4 kg/hm
2.
Because soybean No. 2 cauline leaf grow prolificallies are raised in Shandong, leaf amount is large, and nutrition content is high.Full-bloom stage plant, in dry matter, crude protein content can reach 19.7 %, ADF content 36.1 %, NDF content 45.6 %.And good palatability, be the high quality grass of Herbivores.
variety yield shows
Soybean No. 2 regional experiment of variety yield result are raised in Shandong
Within 2011 to 2012, stand in the examination of Jinan district, the examination of Tai'an district is stood and the regional experiment of variety result of herbage experiment station, Wudi shows, be check variety with wild soybean, cradle at full-bloom stage, within 2011, green forage yield on average reaches 32085.1 kg/hm
2, hay yield reaches 9572.7 kg/hm
2, grass yield and contrast ratio all reach significance level; Within 2012, green forage yield on average reaches 31106.7 kg/hm
2, hay yield on average reaches 9453.0 kg/hm
2, seriously flood except flood except test site, Wudi is caused because of heavy rain, Jinan and examination station, Tai'an district grass yield all reach significance level.
Soybean No. 2 production test yield result are raised in Shandong
(2) production test
2012 to 2013 in Wudi, the Tai Er village and Zhangqiu carry out production test, result of the test shows, and take wild soybean as check variety, cradles at full-bloom stage, and 3 test sites, in the test data of totally 6 years, have the yield data difference of 5 years to reach significance level.Wherein, fresh grass in 2012 and hay average yield are respectively 31299.4 kg/hm
2with 9412.5 kg/hm
2, than contrast raising 10.1% and 15.3%, fresh grass in 2013 and hay average yield are respectively 30907.1 kg/hm
2with 9350.6 kg/hm
2, than contrast raising 9.4% and 12.8%.
Adaptation zone: be suitable for growing in more than, middle fertility deep at the whole province's soil layer, sun-drenched neutrality or alkalescent soil.
Cultural technigue:
Plot: need deep plough and rake, loosing soil is smooth.Will use sufficient base fertilizer during whole ground, per hectare can use fertilizer 30000 kg, or uses composite fertilizer 300 kg.
Sowing: sow in spring or summer sowing.Drilling or bunch planting; Drill spacing 40 ~ 50 cm, seed level 3 ~ 5 cm, seeding quantity 30 ~ 45 kg/hm
2; Bunch planting distance between rows and hills is 30 ~ 40 cm, seed level 3 ~ 5 cm, seeding quantity 22.5 ~ 30 kg/hm
2.
Field management: want timely intertill and clean tillage after emerging, according to soil moisture content determination irrigation time and number of times.Have stronger premunition, damage by disease and insect is few.
Above-described embodiment is the present invention's preferably embodiment; but embodiments of the present invention are not by the restriction of embodiment; other is any do not deviate from Spirit Essence of the present invention and principle under make change, modification, combination, substitute, simplify and all should be equivalent substitute mode, be included within protection scope of the present invention.
Claims (7)
1. a selection for forage grass type soybean, is characterized in that comprising the following steps:
38 are handed over to be maternal with beans, wild soybean DYKSW-3 is male parent, carry out artificial pollination, obtain first-filial generation seed, selfing after plantation, the independent sowing of every strain obtains second filial generation seed, plantation, from second filial generation plant, screen upright and that branch amount is many individual plant reserve seed for planting, and carry out inbreeding of more generation purifying, to obtain final product.
2. selection according to claim 1, is characterized in that from second filial generation, filters out upright and stem branch amount and reserve seed for planting more than the individual plant of more than 10 from segregative generation.
3. selection according to claim 1 and 2, is characterized in that with the yield and quality of plant nutrition body for breeding goal, selects the strain that output is high, product are of fine quality to reserve seed for planting from the strain after inbreeding of more generation purifying.
4. selection according to claim 3, is characterized in that screening individual plant forage yield is the highest, crude protein content is reserved seed for planting higher than the strain of 18%.
5. selection according to claim 1, is characterized in that comprising the following steps:
(1) plant first-filial generation seed, the independent sowing of every strain, obtains second filial generation seed;
(2) by individual plant branch plantation second filial generation seed, in the plant of trait segregation, carry out mark and independent sowing to upright individual plant, and carry out the observation of branch amount between individual plant and compare, choosing upright and stem branch amount higher than the individual plant of 10 is hybridization three generations seed;
(3) plantation hybridization three generations seed, continuing to choose upright and stem branch amount higher than the individual plant of 10 is hybridization four generation seed;
(4) plant hybridization four generation seed, carry out the test of full-bloom stage forage yield and nutrient content, screening individual plant forage yield is the highest, and crude protein content, higher than the strain of 18%, is gathered in the crops hybridization five generation seed, to be obtained final product.
6. selection according to claim 1, is characterized in that the maternal interlacing plantation of male parent.
7. selection according to claim 1; it is characterized in that the mode that artificial pollination adopts " late emasculation, early pollination ", the bud that dew is purple selected by maternal the cultivated soybean, 4-6 point emasculation in the afternoon; with the column cap that nearest blade protection is exposed, pollinated immediately after morning next day, 7-9 point got pollen.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107018804A (en) * | 2017-05-31 | 2017-08-08 | 六枝特区家香农林种养殖农民专业合作社 | A kind of breeding method of tomato panax ginseng fruit |
| CN109089501A (en) * | 2018-08-09 | 2018-12-28 | 吉林农业科技学院 | A kind of research methods that diammonium hydrogen phosphate Different Fertilization amount influences big thousand life |
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| CN1271383A (en) * | 1997-07-07 | 2000-10-25 | 埃克斯希德遗传有限公司 | Animal feed with low phytic acid, oil burdened and protein laden grain |
| WO2004006659A1 (en) * | 2002-07-11 | 2004-01-22 | Monsanto Technology, Llc | High yielding soybean plants with increased seed protein plus oil |
| CN101312644A (en) * | 2005-09-07 | 2008-11-26 | 孟山都技术有限公司 | Agronomically elite soybeans with high beta-conglycinin content |
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Patent Citations (3)
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| CN1271383A (en) * | 1997-07-07 | 2000-10-25 | 埃克斯希德遗传有限公司 | Animal feed with low phytic acid, oil burdened and protein laden grain |
| WO2004006659A1 (en) * | 2002-07-11 | 2004-01-22 | Monsanto Technology, Llc | High yielding soybean plants with increased seed protein plus oil |
| CN101312644A (en) * | 2005-09-07 | 2008-11-26 | 孟山都技术有限公司 | Agronomically elite soybeans with high beta-conglycinin content |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107018804A (en) * | 2017-05-31 | 2017-08-08 | 六枝特区家香农林种养殖农民专业合作社 | A kind of breeding method of tomato panax ginseng fruit |
| CN109089501A (en) * | 2018-08-09 | 2018-12-28 | 吉林农业科技学院 | A kind of research methods that diammonium hydrogen phosphate Different Fertilization amount influences big thousand life |
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