CN107455254B - Corn selects system, method with the polymerization of group's major clique - Google Patents
Corn selects system, method with the polymerization of group's major clique Download PDFInfo
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- CN107455254B CN107455254B CN201710681174.2A CN201710681174A CN107455254B CN 107455254 B CN107455254 B CN 107455254B CN 201710681174 A CN201710681174 A CN 201710681174A CN 107455254 B CN107455254 B CN 107455254B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
The present invention relates to breeding techniques, a kind of corn is specifically provided and selects system, method with the polymerization of group's major clique, its are as follows: two excellent and complementary pure lines of comprehensive agronomy character are selected in same corn monoid first and are hybridized, then by doubling to filial generation progress selfed breeding or haploid induction, the new pure lines that comprehensive agronomy character is better than its parents are finally selected.A kind of corn provided by the present invention selects system, method with the polymerization of group's major clique, and basis selects based material to belong to same monoid, and comprehensive agronomy character has complementary advantages, and the new pure lines selected are superior to two with group parent in above-mentioned character.This method accelerates abundant and new varieties the breeding work that China is precocious, resistance to close, suitable machine receives germ plasm resource, and for breeder provide it is a kind of it is fast and effeciently continuous improve, surmount, gather the method for fine germplasm resources, so that ensure that the plant breeding such as China's corn are studied stablizes sustainable development.
Description
Technical field
The present invention relates to breeding technique, specifically a kind of corn selects system, method with the polymerization of group's major clique.
Background technique
Elite inbred breeding is actually exactly constantly to improve, surmount, gathering the process of fine germplasm resources, and successfully group
Premise with the how anti-cenospecies of high yield and high quality.Currently, China most there is an urgent need to be that precocious High planting is good, lodging resistance is strong, suitable machine
The excellent germ plasm resources of Comprehensive Traits such as receipts, yield potentiality height.For the needs that adapt to the situation, we manage according to maize genetic breeding
By in conjunction with breeding practice, proposing a kind of same group's major clique polymerization choosing is method.This method not only can solve what China was badly in need of at present
The breeding for the germ plasm resources such as precocious High planting is good, lodging resistance is strong, yield potentiality is high works, and can accelerate precocious, resistance to close, anti-
, the breeding work for the corn variety that preferably machine is received, yield is high.
Summary of the invention
The present invention provides a kind of corn and selects system, method with the polymerization of group's major clique, selected in same corn monoid first two it is comprehensive
It closes the excellent and complementary pure lines of economical character to be hybridized, then by carrying out selfed breeding or haploid induction to filial generation
It doubles, finally selects the new pure lines that comprehensive agronomy character is better than its parents.
The pure lines are the general name of the system as composed by genetically uniform homozygous individual, are self-mating system or DH system.
A kind of corn of the present invention selects system, method with the polymerization of group's major clique, specifically includes the following steps:
A, it is identified by pedigree information analysis or molecular engineering, lists pedigree and understand, belong to the same Heterotic Groups, and
Genetic relationship is closer to be sheerly several, selects excellent pure lines therein N number of；
B, the investigation of comprehensive agronomy character is carried out to N number of excellent pure lines respectively, be selected in one or more comprehensive agronomy characters
Two excellent pure lines having complementary advantages are hybridized, and select based material using filial generation as constructed basis；
C, it selects based material to be continuously selfed and/or carry out haploid induction and double on basis, is largely sheerly；
D, selected from above-mentioned a large amount of pure lines by Parent advantage condense together and comprehensive agronomy character better than two
With the new pure lines of group parent.
Wherein, the comprehensive agronomy character refers to any in plant height, fringe position, lodging resistance, disease resistance, High planting, ripe phase etc.
Kind is several.
Wherein, the excellent pure lines refer to the excellent backbone pure lines that comprehensive agronomy character is good, coordinate force is high；It is preferred that it is selected
Standard are as follows: the pure lines cenospecies that group has been allotted promotes and applies 1,000,000 mu or more in production, obtains large area production inspection
Test or the pure lines group allots 3 kinds above by authorization.
Wherein, the continuous selfing preferably selects system, method to carry out with " tall and big tight " etc..
Wherein, the continuous selfing preferably continuous selfing 2-3 generation.
The present invention also provides the corns to select application of the system, method in plant variety breeding, the plant with the polymerization of group's major clique
Object is any one of self-pollinated plant, Constantly allogamous plant, cross-pollinatd plant, preferably corn.
The beneficial effects of the present invention are:
A kind of corn provided by the present invention selects system, method with the polymerization of group's major clique, and basis selects based material to belong to same monoid,
And have complementary advantages in comprehensive agronomy character, the new pure lines selected are superior to two with group parent in above-mentioned character.
A kind of same group's major clique polymerization choosing provided by the present invention is that method accelerates China's precocity, resistance to close, suitable machine sowing matter money
The breeding of the abundant and new varieties in source works, and for breeder provide it is a kind of it is fast and effeciently continuous improve, surmount, gather it is excellent
The method of good germ plasm resource, so that ensure that the plant breeding such as China's corn are studied stablizes sustainable development.
Specific embodiment
The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..Those skilled in the art exists
In the case where without departing substantially from spirit of the invention and spirit, the present invention can be carry out various modifications and be replaced.
Kind capital 724, capital 464 involved by embodiment, capital MC01 and capital 4055 are known kind, the public can from Germplasm Bank or
Breeding unit is introduced a fine variety.
Embodiment 1
Capital 72464 be with same group's major clique capital 724 and 464 phase mutual cross of capital for polymerization choosing be made of basic material breeding it is excellent
Good self-mating system.
(1) self bred inbred line capital 724, capital 464 derive from the choosing system that cenospecies X1132x Hun Fen group of the U.S. is basic material,
Belong to the same Heterotic Groups, the closer self-mating system of genetic relationship.
(2) self bred inbred line capital 724, capital 464 are the excellent Inbred Lines that Comprehensive Traits are excellent, coordinate force is high.Make respectively
For female parent, group allots the superior corns new varieties such as capital section 968, NK718, obtains large area production testing.Wherein, capital section 968 in
By country's authorization, 60,000,000 mu or more are promoted and applied in production, becomes the leading big product for occupying national front three within 2011
Kind；NK718 passes through the authorization of the provinces and cities such as Inner Mongol, Shaanxi, Shanxi, Shandong, Hebei respectively or introduces a fine variety, and 700 are promoted and applied in production
Ten thousand mu or more.
(3) 2006 year spring is in Beijing, by finding to capital 724 and the investigation of 464 field comprehensive agronomy character of capital, self bred inbred line
Capital 724 is big with fruit ear, fringe position is higher, lodging resistance is weak, is not easy the features such as dense planting, and self bred inbred line capital 464 has fringe position inclined
Features, the two such as low, High planting, lodging resistance be strong have complementary advantages in terms of the economical characters such as fringe position, High planting and lodging resistance.
(4) 2006 year winter planted Inbred Lines capital 724 and capital 464 in Hainan, and completed the hybridization of 724 × capital of capital 464.
After maturation, threshing is mixed, building choosing is basic material.
(5) 2007 year spring, planting the choosing of 724 × capital of capital, 464 convergent cross was 3000 plants of basic material or more in Beijing, was pressed
It selects system, method strictly to select to eliminate according to tall and big sternly etc., obtains 60 satisfactory fruit ears.
60 satisfactory fruit ears are planted in Sanya within (6) 2007 years, select system, method to select fine individual plant according to tall and big sternly etc.
Selfing.
(7) 2008 year spring continued to carry out head progeny row selection under 6000 plants/acre of density in Beijing, filters out and meets the requirements
200, fruit ear.
(8) 2008 year winter carried out haploid induction using DH breeding technique to 200 selected fruit ears in Sanya, according to
Seed Navajo mark property sub-elects monoploid seed 3000.
(9) 2009 year spring planted 3000 monoploid seeds in Beijing.Florescence is according to haplobiont loose powder situation
It is selfed, after mature, harvest self-fertility DH is sheerly 240 plants.
(10) 2009 year winter planted 240 plants of DH pure lines in Hainan, carries out DH assemblage and closes economical character investigation and screening mirror
It is fixed.By reflecting to the resistance to close anti-fall investigation and analysis such as spinning concentrations, plant plant height, aerial root quantity, stalk thickness and flexibility
Surely stronger 40 DH1, DH2, DH3 ... the DH40 of excellent DH system of High planting, lodging resistance are filtered out.And spin the phase in loose powder, with
The self-mating systems such as capital 2416, capital 2418 are assembled, and DH1 × capital 2416, DH2 × capital 2416, DH3 × capital 2416 ... DH40 are obtained
× capital 2416 and DH1 × capital 2418, DH2 × capital 2418,2418 cross combination of DH3 × capital 2418 ... DH40 × capital 80.
80 cross combinations that (11) 2010 year spring allotted group carry out multiple spot height in places such as Beijing, Henan, Jilin
Produce yield stability identification.And to the ripe phase, the economical characters such as empty bar rate is low, fruit ear size, yield investigate and analyse, evaluation and screening goes out
Cross combination DH1 × capital 2418 that yield is high, the ripe phase is early, High planting is good, lodging resistance is strong, i.e. corn variety MC817.The kind
It is authorized by Hebei, has that yielding ability yield stability is good, is suitable for dense planting, high resistant to lodging, simple grain program request and mechanized harvest etc.
Advantage.
The excellent DH system was named as capital 72464 in (12) 2011 years.Capital 72464 is having both its big fruit ear in maternal capital 724, production
Measure coordinate force it is high on the basis of, while the ripe phase, fringe position, High planting, in terms of also superior to its 2 capital with group parent
724 and capital 464, there is apparent improved effect.
Embodiment 2
Capital B547 is Corn Rearch Center, Beijing Farming & Forestry Research Academy's self bred inbred line, is that capital MC01 and 4,055 two, capital are excellent certainly
The hybridization polymerization choosing system that friendship is, wherein Elite inbred capital MC01 is adapted for machine receipts corn variety Jing Nong section 728 (the careful jade of state
2012003, beautiful female parent 2014006) is examined in capital, have many advantages, such as it is resistance to it is close, precocious, plant type is ideal, seed dewatering speed is fast, disadvantage
It is that lodging tolerance is slightly poor, disease resistance is slightly poor.Another Elite inbred capital 4055 is the female parent of corn variety MC220, is had
The advantages that high resistant to lodging, resistance, excellent comprehensive agronomy character, the disadvantage is that it is late-maturing, High planting is poor.Two are assembled in Beijing within 2006
A Elite inbred cross combination obtains F1Seed, the same year are selfed in Hainan and obtain F2Seed, 2007-2008 utilize " tall and big
Sternly " choosing is technology in Beijing by F2And F3It is selfed in 2 generations and selects, realizing excellent gene, quickly and efficiently accumulation is poly-
It closes, filters out the excellent F of comprehensive agronomy character4Strain；2008 in Hainan in F4While strain selfing is homozygous, capital is utilized
2416, the Testers such as capital 92 carry out combining ability test, the higher F of further screening coordinate force to it5Strain；2009 in north
Capital utilizes DH breeding technique by F5Strain is induced, and monoploid is obtained；Monoploid is carried out doubling to obtain in Hainan within 2009
DH system；DH system is expanded in Beijing within 2010 numerous and carry out stringent screening and assessment, filtered out precocious, resistance to close, disease-resistant anti-fall excellent
Good DH system, and it is named as capital B547.
The groups such as capital B547 and Inbred Lines capital 2416, capital 92, prosperous 7-2, capital 24 allot 10 cross combinations in Beijing, river
The places such as south, Jilin carry out the identification of multiple spot stable high yield.And it is comprehensive to ripe phase, yield, disease resistance, lodging resistance, High planting etc.
Character is investigated and analysed, and the cross combination capital B547 that evaluation and screening yield is high, the ripe phase is early, High planting is good, lodging resistance is strong ×
Capital 2416, i.e. corn variety MC812.The kind is authorized by Beijing, compact with plant type, ripe morning phase, yielding ability
The advantages such as yield stability is good, be suitable for dense planting, disease resistance is strong, height is resistant to lodging.
Capital B547 have both its female parent capital MC01 it is resistance to it is close, precocious, plant type is ideal on the basis of, while lodging tolerance,
Disease resistance etc., with group parent capital MC01 and capital 4055, has apparent improved effect also superior to its 2.Capital B547 seedling leaf
Sheath purple, blade, leaf margin green, Tassel-Branch few (2-3) and major branch is long, anther darkviolet, glume lavender；Plant type is tight
It gathers, plant height 164cm, Ear height 82cm, filigree is red, and fruit ear fringe handle is of moderate length, and the long cartridge type of fruit ear, bract length is long, spike length
16.0cm, 14 row of tassel row number, grain weight per panicle 110g or so, cob is red, seed yellow, half dent type, 100-grain weight 351g.The production of hybrid seeds
Production is suitable as female parent, every 666.7m2 hybrid seed yield 450kg or so.
Claims (1)
1. a kind of corn selects system, method with the polymerization of group's major clique, which is characterized in that its are as follows: two are selected in same corn monoid first
The excellent and complementary pure lines of a comprehensive agronomy character are hybridized, then by carrying out selfed breeding or monoploid to filial generation
Induction doubles, and finally selects and is better than the new pure lines of its parents in comprehensive agronomy character；The comprehensive agronomy character refer to plant height,
Any one or more of fringe position, lodging resistance, disease resistance, High planting, ripe phase；
Specifically includes the following steps:
A, it is identified by molecular engineering, lists pedigree and understand, belong to cenospecies X1132x Hun Fen group of the U.S., and hereditary relationship
Relationship is closer to be sheerly several, selects excellent pure lines therein N number of；The excellent pure lines refer to that comprehensive agronomy character is good, cooperates
The high excellent backbone pure lines of power, the selection criteria of excellent pure lines are as follows: the pure lines cenospecies that group has been allotted is promoted in production
Using 1,000,000 mu or more or the pure lines, group allots 3 kinds above by authorization；
B, the investigation of comprehensive agronomy character is carried out to N number of excellent pure lines respectively, is selected in advantage in one or more comprehensive agronomy characters
Complementary two excellent pure lines capital 724 and capital 464 are hybridized, and select based material using filial generation as constructed basis；
C, it selects based material to be continuously selfed or carry out haploid induction and double on basis, is largely sheerly；The continuous selfing
Sternly system, method is selected to carry out with tall and big；
D, it selects and Parent advantage condenses together and comprehensive agronomy character is better than two same group from above-mentioned a large amount of pure lines
The new pure lines capital 72464 of parent.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116406614A (en) * | 2021-12-31 | 2023-07-11 | 北京市农林科学院 | Corn germplasm population construction and line selection method for rapid enrichment of multiple excellent genes |
| CN115316266B (en) * | 2022-09-05 | 2023-09-08 | 新疆农垦科学院 | Crop population improvement and variety breeding method |
| CN116918699A (en) * | 2023-09-05 | 2023-10-24 | 河北省农林科学院旱作农业研究所 | Silage maize inbred line breeding method |
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| CN101828519A (en) * | 2009-12-15 | 2010-09-15 | 丹东登海良玉种业有限公司 | Corn homologous sister inbred line breeding method |
| CN102138517A (en) * | 2010-12-17 | 2011-08-03 | 贵州省毕节地区农业科学研究所 | Breeding method for maize variety |
| CN103782895A (en) * | 2014-02-11 | 2014-05-14 | 山东省农业科学院玉米研究所 | Synthetic method of original colony for corn breeding for reciprocal recurrent selection |
| CN104521739A (en) * | 2014-12-04 | 2015-04-22 | 刘传永 | Breeding method of hybrid corn seeds |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101828519A (en) * | 2009-12-15 | 2010-09-15 | 丹东登海良玉种业有限公司 | Corn homologous sister inbred line breeding method |
| CN102138517A (en) * | 2010-12-17 | 2011-08-03 | 贵州省毕节地区农业科学研究所 | Breeding method for maize variety |
| CN103782895A (en) * | 2014-02-11 | 2014-05-14 | 山东省农业科学院玉米研究所 | Synthetic method of original colony for corn breeding for reciprocal recurrent selection |
| CN104521739A (en) * | 2014-12-04 | 2015-04-22 | 刘传永 | Breeding method of hybrid corn seeds |
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