CN102308747B - Method for carrying out variety purity or trueness identification on corn by utilizing purple germ mark - Google Patents
Method for carrying out variety purity or trueness identification on corn by utilizing purple germ mark Download PDFInfo
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- CN102308747B CN102308747B CN201110149085.6A CN201110149085A CN102308747B CN 102308747 B CN102308747 B CN 102308747B CN 201110149085 A CN201110149085 A CN 201110149085A CN 102308747 B CN102308747 B CN 102308747B
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Abstract
The invention discloses a corn with a purple germ mark, wherein the color of the germ of the corn is purple. The invention further discloses a method for carrying out the purity identification on a corn hybrid by utilizing the purple germ mark. The method comprises the following steps of: carrying out hybridization by using a selfing line with the colorless germ as a female parent and a selfing line with the purple germ as a male parent, mixing and threshing; and then randomly extracting grains, observing whether the germs of the grains are purple or not with naked eyes, and calculating the purity of the hybrid seeds of the corn. The invention further discloses a breeding method of a corn variety with the purple germ. The breeding method comprises hybrid breeding and back cross breeding processes. The method for carrying out the purity identification on the corn hybrid can carry out the purity identification on the corn seeds only by the virtue of the naked eyes by utilizing the purple germ mark, is simple and convenient, and has low cost and high measurement speed; moreover, the purple property of the germ is controlled by a single dominant gene; and the germ has stable property expression and reliable identification results.
Description
Technical field
The invention belongs to crop varieties Purity method field, be specifically related to utilize purple embryo labels to carry out the method for Purity Testing of Maize Cultivars or authenticity identification; Also relate to the breeding method with the corn variety of purple embryo labels.
Background technology
Corn is cross pollinated plant, in corn seed producing process, because quarantine measures are not strict, is vulnerable to the invasion of external zasiokaurin, causes and mixes; Or because maternal artificial emasculation is incomplete, produce selfed seed; Or mechanical admixture that when results causes etc., has a strong impact on the quality of corn hybrid seed, finally Maize Production is caused to very big loss.The main method of corn hybrid seed Purity or authenticity identification has: the evaluation in seedling stage of (1) field, and this kind of method cycle is oversize, and therefore poor reliability substantially need not; (2) Isozyme Electrophoretic or DNA fingerprinting are identified, this kind of method complicated operation needs certain equipment and those skilled in the art, and general unit is difficult to possess this condition, wastes time and energy, and cost is high.
If can with naked eyes to seed with outward appearance Marker Identification corn purity should be simple and the low method of cost.Through retrieval, not have discovery by seed with the method for purple embryo labels naked eyes Direct Identification corn hybrid seed purity.
Summary of the invention
The object of the invention is to provide a kind of corn with purple embryo labels.
Another object of the present invention is to provide the method for utilizing purple embryo labels to carry out purity of hybrid maize evaluation.
The present invention's the 3rd object is to provide utilizes purple embryo labels to carry out the method for corn variety authenticity identification.
The present invention's the 4th object is to provide a kind of breeding method of the corn variety with purple embryo labels.
For achieving the above object, technical scheme of the present invention is:
A kind of corn with purple embryo labels of the present invention, described in it, the plumule color of corn is purple.The Purpled traits of plumule is controlled by single dominant gene, and the corn that the plumule of take is purple is to the colourless corn pollination of plumule, and the plumule of the contemporary gained seed of pollinating shows as purple, can be used for the purity that naked eyes are intuitively identified corn seed.
Utilize above-mentioned purple embryo labels to carry out the method for purity of hybrid maize evaluation, comprise take plumule as colourless selfing be maternal, the selfing that the plumule of take is purple is paternal hybrid, results hybridization fruit ear, mixes threshing; Then randomly draw seed, whether its plumule of naked-eye observation is purple, counts, and then calculates the purity of corn hybrid seed; The purity computing formula of corn hybrid seed is:
In corn hybrid seed purity (%)=sample with quantity/sample seed sum * 100% of purple plumule seed.
The corn inbred line with purple plumule described in said method can be yellow comprehensive 721, yellow comprehensive 721 belong to Zea maize seed (Zea mays L.), on March 13rd, 2011, be preserved in (address:, deposit number is: CCTCC No:P201104, Chinese Typical Representative culture collection center Wuhan, China Wuhan University).
Utilize purple embryo labels to carry out the method for corn variety authenticity identification, whether the kind that is purple for plumule, be this kind according to the plumule color identification on seed, if plumule is purple, is exactly this kind, as Huang combines 721; If its plumule is not purple, not so just this kind.
With a breeding method for the corn inbred line of purple embryo labels, can cultivate by the method for crossbreeding or back cross breeding.
Cross breeding method described in above-mentioned breeding method, comprise that with the colourless selfing of plumule be one of parent, with the selfing with purple plumule, be one of parent hybridization, then carry out selfing, in gained self progeny, select the seed that economical character is good and plumule is purple, carry out continuously the selfing of 5～8 generations, can obtain plumule is the new inbred line of purple.
Back cross breeding method described in above-mentioned breeding method, comprise that with the colourless selfing of plumule be recurrent parent, with the selfing with purple plumule, it is male parent non-recurrent parent, backcross, in backcross progeny, selecting plumule is the seed of purple and the material that other proterties approach recurrent parent, in 4～8 generations of continuous backcross, can obtain the new inbred line of purple plumule.
The inbred line with purple plumule described in above-mentioned breeding method is yellow comprehensive 721.
With the breeding method of the corn single cross hybrid of purple embryo labels, the corn inbred line that the plumule of take is purple is male parent, take plumule as colourless corn inbred line be hybridization of female parent, the hybrid seed of gained is the corn single cross hybrid that plumule is purple.
The inbred line with purple plumule described in above-mentioned breeding method can be yellow comprehensive 721.
Described corn self rotary series yellow combines 721, obtains by the following method:
(1) take prosperous 7-2 as maternal, take respectively from 330, H92-1 and No. 9 tropical corns hybridize as male parent, must hybridize F
1generation;
(2) take prosperous 7-2 as maternal, with three hybridization F of gained in step (1)
1the pollen mixture in generation is paternal hybrid, obtains the C of Huang Zong colony
0in generation, find the seed that wherein plumule is purple;
(3) take the plumule of gained is purple in step (2) seed for basis, utilize pedigree method to carry out roguing selfing, select the seed that in offspring, plumule is purple, carry out continuously the selfing of 8 generations, be bred as with the inbred line of purple plumule yellow comprehensive 721.
Prosperous 7-2 described in above-mentioned breeding method and be all the inbred line of producing extensive use from 330; Described crop investigations institute of H92-1 Yin Zi Shanxi Province Academy of Agricultural Sciences; No. 9 tropical corn derives from Su Wan colony hybrid, 2003 Nian Yinzi Jinzhou City Academy of Agricultural Sciences.Concrete breeding process is as follows: calendar year 2001 be female parent at Cangzhou, Hebei Yi Chang 7-2, take respectively from 330, H92-1, No. 9 tropical corns hybridize as male parent, gathers in the crops to obtain F
1generation; Be maternal in Hainan Yi Chang 7-2 winter in the same year, with three F
1the pollen mixture in generation is that male parent is hybridized, seed mixture after results, the C of get Huang Zong colony
0generation, the seed that is purple at the favorite outer discovery plumule of seed; Then the seed that the plumule of take is purple is basis, in 2002～2005, through Cangzhou and two places, Hainan continuous 8 generations, select plumule purple, the good individual plant selfing of economical character, winter in 2005, in Hainan, obtaining plumule is the good stable inbred line of isozygotying of purple, economical character, and called after is yellow combines 721.
Compared with prior art, the present invention has advantages of: (1), seed purity identification method of the present invention are simple and convenient, do not need instrument and equipment and professional skill, only with naked eyes, can measure, and cost is low, and finding speed is fast; (2) accurately and reliably, because purple plumule proterties is that single dominant gene is controlled, so the Characters is stable, reliable results for Purity method of the present invention.
Accompanying drawing explanation
Fig. 1. with the contrast photo of the corn variety of purple embryo labels; Wherein 1 is that Huang comprehensive 721,2 is C618, and 3 is deep blue jade 18.
Embodiment
Below in conjunction with embodiment, the invention will be further described.
The Genetic identification test of embodiment 1 Maize Purple plumule proterties
Carry out as follows:
(1) take plumule as colourless corn inbred line C618 (seeing Fig. 1-2) is as maternal, corn self rotary series yellow that the plumule of take is purple combine 721 (seeing Fig. 1-1) on March 13rd, 2010 in the center preservation of Chinese Typical Representative culture collection, preserving number is CCTCC No:P201104) be paternal hybrid, obtaining plumule color is the hybridization F of purple
1for (deep blue jade 18) seed (seeing Fig. 1-3); The self-crossing seedling P248 wherein finding in C618 Shi Yi U.S. crossbreed 78599 colonies is female parent, take Zheng 58 as paternal hybrid, must hybridize F
1generation; Then take Zheng 58 as female parent, with F
1on behalf of male parent 1 generation that backcrossed, then backcross progeny is carried out to the inbred line that the selfing of 6 generations is bred as continuously;
(2) plantation F
1in generation, selfing, must hybridize F
2for seed;
(3) by gained F
2for seed mixing threshing, therefrom choose at random 500 seed observation plumule colors, counting, result plumule is that the seed of purple is 372, plumule is 128 of colourless seeds, meets the segregation ratio of 3: 1, and the Purpled traits that plumule is described is that single dominant gene is controlled.
The approval test that backcrosses of embodiment 2 maize germ Purpled traits heredity
Carry out as follows:
(1) take C618 as maternal, it is paternal hybrid that the Huang of take combines 721, obtains the F that plumule color is purple
1for hybrid seed;
(2) plantation F
1in generation, then take C618 as maternal, the hybridization F that the plumule of take is purple
1on behalf of male parent, backcross, gather in the crops to obtain first backcross generation seed;
(3) mix threshing, choose at random 1000, observation plumule color, counting, the seed quantity that result plumule is purple is 504, plumule is that colourless seed quantity is 496, meet the segregation ratio of 1: 1, illustrate that purple plumule is to be controlled by single dominant gene in heredity.
The cultivation of the corn inbred line that embodiment 3, plumule color proterties are purple
Carry out as follows:
(1) take LX9801 (cultivation of corn research institute of the Agriculture in Shandong Province academy of sciences) as maternal, it is paternal hybrid that the Huang of take combines 721, and obtaining plumule is the hybridization F of purple
1for seed;
(2) plantation F
1in generation,, then with the hybridization F of purple plumule
1on behalf of female parent, take LX9801 as male parent, backcross, gather in the crops to obtain first backcross generation seed, choose the seed that plumule is purple;
(3) choose the first backcross generation seed plantation with purple embryo mark, therefrom select good stress resistance, coordinate force is high and with the fine individual plant of purple embryo labels, continuously 5 generations of selfing, select the seed that in offspring, plumule is purple, cultivate the corn inbred line that purple plumule is purple " yellow comprehensive 801 ".
Embodiment 4 utilizes purple embryo labels to identify the purity test of corn hybrid seed
2010 in Cang Yuzhong industry experiment station, Cangzhou, Hebei, take corn inbred line Zheng 58 (Henan Academy of Agricultural Sciences's cultivation) as maternal, in the loose powder phase, Huang comprehensive 721 and Zheng's 58 pollen is mixed, give Zheng 58 pollinations, when ripe, gather in the crops 2436 of seeds, randomly draw 300 and carry out naked eyes situ appraisal, draw wherein with 165 of the seeds of purple plumule, not with 135 of the seeds of purple plumule, according to the formula of seed amount/sample seed sum * 100% of purple plumule in corn hybrid seed purity (%)=sample, calculating hybrid rate is 55%; Then sample is identified with electrophoresis, show that hybrid rate is 55%, qualification result is in full accord.Illustrate and utilize purple plumule to identify corn seed purity accurately and reliably.
Claims (1)
1. with a breeding method for the corn inbred line of purple embryo labels, it is characterized in that cultivating by back cross breeding method; Described back cross breeding method, carry out in accordance with the following steps:
(1), take LX9801 as maternal, it is paternal hybrid that the Huang of take combines 721, obtaining plumule is the hybridization F of purple
1for seed; Wherein LX9801 is the corn inbred line that corn research institute of the Agriculture in Shandong Province academy of sciences cultivates; Yellow comprehensive 721 belong to Zea maize seed (Zea mays L.), on March 13rd, 2011, are preserved in Chinese Typical Representative culture collection center, and deposit number is: CCTCC No:P201104;
(2), plantation F
1in generation,, then with the hybridization F of purple plumule
1on behalf of female parent, take LX9801 as male parent, backcross, gather in the crops to obtain first backcross generation seed, choose the seed that plumule is purple;
(3), choose the first backcross generation seed plantation with purple embryo mark, therefrom select good stress resistance, coordinate force is high and with the fine individual plant of purple embryo labels, continuously 5 generations of selfing, select the seed that in offspring, plumule is purple, cultivate the corn inbred line that plumule is purple " yellow comprehensive 801 ".
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| Application Number | Priority Date | Filing Date | Title |
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| CN201110149085.6A CN102308747B (en) | 2010-06-25 | 2011-06-03 | Method for carrying out variety purity or trueness identification on corn by utilizing purple germ mark |
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| CN 201010209501 CN101897292A (en) | 2010-06-25 | 2010-06-25 | Breeding method of hybrid corn seeds with embryo labels |
| CN201110149085.6A CN102308747B (en) | 2010-06-25 | 2011-06-03 | Method for carrying out variety purity or trueness identification on corn by utilizing purple germ mark |
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| CN102308747B true CN102308747B (en) | 2014-11-12 |
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| CN101897292A (en) * | 2010-06-25 | 2010-12-01 | 河北沧玉种业科技有限公司 | Breeding method of hybrid corn seeds with embryo labels |
| CN102972285B (en) * | 2012-12-05 | 2014-03-26 | 湖南杂交水稻研究中心 | Breeding method of hybrid crops |
| CN106718857A (en) * | 2016-11-29 | 2017-05-31 | 云南瑞禾种业有限公司 | Efficient corn variety circulation selection |
| CN108362649A (en) * | 2017-12-18 | 2018-08-03 | 温州科技职业学院 | A method of the detection purity of hybrid of the color based on Cauliflower hypocotyl |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1586156A (en) * | 2004-08-19 | 2005-03-02 | 浙江大学 | Breed selecting method for corn with leaf color mark |
| CN1843092A (en) * | 2006-05-18 | 2006-10-11 | 吉林省农业科学院 | Method for selecting and breeding corn new-bred through hybridized induction of unisexual seeding material |
| CN101897292A (en) * | 2010-06-25 | 2010-12-01 | 河北沧玉种业科技有限公司 | Breeding method of hybrid corn seeds with embryo labels |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1586156A (en) * | 2004-08-19 | 2005-03-02 | 浙江大学 | Breed selecting method for corn with leaf color mark |
| CN1843092A (en) * | 2006-05-18 | 2006-10-11 | 吉林省农业科学院 | Method for selecting and breeding corn new-bred through hybridized induction of unisexual seeding material |
| CN101897292A (en) * | 2010-06-25 | 2010-12-01 | 河北沧玉种业科技有限公司 | Breeding method of hybrid corn seeds with embryo labels |
Non-Patent Citations (1)
| Title |
|---|
| 张建成等.玉米杂交种室内纯度的鉴定方法.《种子世界》.1989,第25卷(第9期),25. * |
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| CN101897292A (en) | 2010-12-01 |
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