CN102308747A - Method for carrying out variety purity or trueness identification on corn by utilizing purple germ mark - Google Patents
Method for carrying out variety purity or trueness identification on corn by utilizing purple germ mark Download PDFInfo
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- CN102308747A CN102308747A CN2011101490856A CN201110149085A CN102308747A CN 102308747 A CN102308747 A CN 102308747A CN 2011101490856 A CN2011101490856 A CN 2011101490856A CN 201110149085 A CN201110149085 A CN 201110149085A CN 102308747 A CN102308747 A CN 102308747A
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Abstract
The invention discloses a corn with a purple germ mark, wherein the color of the germ of the corn is purple. The invention further discloses a method for carrying out the purity identification on a corn hybrid by utilizing the purple germ mark. The method comprises the following steps of: carrying out hybridization by using a selfing line with the colorless germ as a female parent and a selfing line with the purple germ as a male parent, mixing and threshing; and then randomly extracting grains, observing whether the germs of the grains are purple or not with naked eyes, and calculating the purity of the hybrid seeds of the corn. The invention further discloses a breeding method of a corn variety with the purple germ. The breeding method comprises hybrid breeding and back cross breeding processes. The method for carrying out the purity identification on the corn hybrid can carry out the purity identification on the corn seeds only by the virtue of the naked eyes by utilizing the purple germ mark, is simple and convenient, and has low cost and high measurement speed; moreover, the purple property of the germ is controlled by a single dominant gene; and the germ has stable property expression and reliable identification results.
Description
Technical field
The invention belongs to crop varieties purity authentication method field, be specifically related to utilize the purple embryo labels to carry out the method for corn variety purity evaluation or authenticity identification; The breeding method that also relates to the corn variety that has the purple embryo labels.
Background technology
Corn is a cross pollinated plant, in the corn seed producing process, because quarantine measures are not strict, is vulnerable to the invasion of external zasiokaurin, causes to mix; Perhaps, produce selfed seed because maternal artificial emasculation is incomplete; Mechanical admixture that causes when perhaps gathering in the crops or the like has a strong impact on the quality of corn hybrid seed, finally Maize Production is caused very big loss.Corn hybrid seed purity is identified or the main method of authenticity identification has: seedling stage is identified that this kind method cycle is oversize in (1) field, and therefore poor reliability basically need not; (2) isoenzymes electrophoresis or dna fingerprinting are identified, this kind method complicated operation needs certain device and those skilled in the art, and general unit is difficult to possess this condition, wastes time and energy, and cost is high.
If can with naked eyes to seed with the outward appearance mark identify that corn purity should be simple and the low method of cost.Through retrieval, not have discovery through on the seed directly identify the method for corn hybrid seed purity with purple embryo labels naked eyes.
Summary of the invention
The object of the invention is to provide a kind of corn that has the purple embryo labels.
Another purpose of the present invention is to provide utilizes the purple embryo labels to carry out the method that purity of hybrid maize is identified.
The present invention's the 3rd purpose is to provide utilizes the purple embryo labels to carry out the method for corn variety authenticity identification.
The present invention's the 4th purpose is to provide a kind of breeding method that has the corn variety of purple embryo labels.
For realizing above-mentioned purpose, technical scheme of the present invention is:
A kind of corn that has the purple embryo labels of the present invention, the plumule color of its said corn is a purple.The purple proterties of plumule is controlled by single dominant gene, is that the corn of purple gives plumule colourless corn pollination with the plumule, and the plumule of the contemporary gained seed of pollinating shows as purple, can be used for the purity that naked eyes are intuitively identified corn seed.
Utilize above-mentioned purple embryo labels to carry out the method that purity of hybrid maize is identified, comprise with plumule being that colourless inbred line is a female parent, the inbred line that with the plumule is purple is a paternal hybrid, and results hybridization fruit ear mixes threshing; Randomly draw seed then, whether its plumule of naked-eye observation is purple, counts, and calculates the purity of corn hybrid seed then; The purity computing formula of corn hybrid seed is:
Band purple plumule seed number/sample seed sum * 100% in corn hybrid seed purity (%)=sample.
The corn inbred line of the band purple plumule described in the said method can be yellow comprehensive 721; Yellow comprehensive 721 belong to Zea corn kind (Zea mays L.); Be preserved in Chinese typical culture collection center (address: Chinese Wuhan Wuhan University) on March 13rd, 2011, deposit number is: CCTCC No:P201104.
Whether utilizing the purple embryo labels to carry out the method for corn variety authenticity identification, is the kind of purple for plumule, be this kind according to the plumule color identification on the seed, if plumule is a purple, is exactly this kind, combines 721 like Huang; If its plumule is not a purple, not this kind so just.
A kind of breeding method of the corn inbred line with the purple embryo labels can be cultivated through the method for crossbreeding or back cross breeding.
Cross breeding method described in the above-mentioned breeding method; Comprise that with the colourless inbred line of plumule be one of parent; Inbred line with band purple plumule is one of parent hybridization, carries out selfing then, and selecting the good and plumule of economical character among the gained self progeny is the seed of purple; Carry out the selfing of 5～8 generations continuously, can obtain plumule is the new inbred line of purple.
Back cross breeding method described in the above-mentioned breeding method; Comprise that with the colourless inbred line of plumule be recurrent parent; Inbred line with band purple plumule is the male parent non-recurrent parent, backcrosses, and in backcross progeny, selecting plumule is seed and other proterties of the purple material near recurrent parent; In 4～8 generations of continuous backcross, can obtain the new inbred line of purple plumule.
The inbred line of the band purple plumule described in the above-mentioned breeding method is yellow comprehensive 721.
Being with the breeding method of the corn single cross hybrid of purple embryo labels, is that the corn inbred line of purple is a male parent with the plumule, is that colourless corn inbred line is a hybridization of female parent with plumule, and the hybrid seed of gained is the corn single cross hybrid that plumule is a purple.
The inbred line of the band purple plumule described in the above-mentioned breeding method can be yellow comprehensive 721.
Described corn self rotary series yellow combines 721, obtains through following method:
(1) be female parent with prosperous 7-2, respectively with from 330, H92-1 and No. 9 tropical corns are that male parent is hybridized, and must hybridize F
1Generation;
(2) be female parent with prosperous 7-2, with three hybridization F of gained in the step (1)
1The pollen mixture in generation is a paternal hybrid, obtains the yellow C of colony of combining
0In generation, find that wherein plumule is the seed of purple;
(3) be that the seed of purple be basis with the plumule of gained in the step (2), utilize pedigree method to carry out the roguing selfing, select that plumule is the seed of purple among the offspring, carry out the selfing of 8 generations continuously, breed the inbred line Huang comprehensive 721 that has the purple plumule.
Prosperous 7-2 described in the above-mentioned breeding method and all be the inbred line that produce to go up extensive use from 330; Described H92-1 draws Academy of Agricultural Sciences's crop investigations institute from the Shanxi Province; No. 9 tropical corns derive from gulf colony of Soviet Union to be mixed kind, draws the Academy of Agricultural Sciences from the Jinzhou City in 2003.Concrete breeding process is following: calendar year 2001, Yi Chang 7-2 be a female parent in Cangzhou, Hebei, respectively with from 330, H92-1, No. 9 tropical corns be that male parent is hybridized, gather in the crops F
1Generation; Winter in the same year, Yi Chang 7-2 was maternal in Hainan, with three F
1The pollen mixture in generation is that male parent is hybridized, and results back seed mixture gets the yellow comprehensive C of colony
0In generation, unexpected discovery plumule is the seed of purple in seed; The seed that with the plumule is purple then is the basis; Selected plumule purple, the good individual plant selfing of economical character in 2002～2005 through Cangzhou and two places, Hainan continuous 8 generations; Obtaining plumule winter in 2005 in Hainan is the good stable inbred line of isozygotying of purple, economical character, and called after is yellow combines 721.
Compared with prior art, the advantage that the present invention has: (1), seed purity identification method of the present invention are simple and convenient, do not need instrument and equipment and professional skill, only can measure with naked eyes, and cost is low, and finding speed is fast; (2) purity authentication method of the present invention is controlled because purple plumule proterties is a single dominant gene accurately and reliably, so The Characters is stable, reliable results.
Description of drawings
Fig. 1. the contrast photo of the corn variety of band purple embryo labels; Wherein 1 is that Huang comprehensive 721,2 is C618, and 3 is deep blue beautiful 18.
Embodiment
Below in conjunction with embodiment the present invention is described further.
The Genetic identification test of embodiment 1 corn purple plumule proterties
Carry out according to following method:
(1) be that colourless corn inbred line C618 (seeing Fig. 1-2) is a female parent with plumule; With the plumule be the corn self rotary series yellow of purple combine 721 (seeing Fig. 1-1) on March 13rd, 2010 in China's typical culture collection center preservation; Preserving number is CCTCC No:P201104) be paternal hybrid, getting the plumule color is the hybridization F of purple
1For (deep blue beautiful 18) seed (seeing Fig. 1-3); Wherein C618 is female parent, is paternal hybrid with Zheng 58 with the self-crossing seedling P248 that is found in U.S. crossbreed 78599 colonies, must hybridize F
1Generation; Then with Zheng 58 be female parent, with F
1Backcrossed for 1 generation on behalf of male parent, again backcross progeny is carried out the inbred line that the selfing of 6 generations is bred continuously;
(2) plantation F
1In generation, selfing must be hybridized F
2For seed;
(3) with gained F
2For seed mixing threshing, therefrom choose 500 seed observation plumule colors at random, counting; Plumule is that the seed of purple is 372 as a result; Plumule is 128 of colourless seeds, meets 3: 1 segregation ratio, and the purple proterties that plumule is described is single dominant gene control.
The approval test of backcrossing of embodiment 2 maize germ purple character inheritances
Carry out according to following method:
(1) be maternal with C618, combining 721 with Huang is paternal hybrid, and getting the plumule color is the F of purple
1For hybrid seed;
(2) plantation F
1Generation is maternal then with C618, is the hybridization F of purple with the plumule
1Backcross on behalf of male parent, gather in the crops the first backcross generation seed;
(3) mix threshing, 1000 of picked at random, observation plumule color; Counting, plumule is that the seed quantity of purple is 504 as a result, plumule is that colourless seed quantity is 496; Meet 1: 1 segregation ratio, explain that the purple plumule is to be controlled by single dominant gene in heredity.
Carry out according to following method:
(1) be female parent with LX9801 (cultivation of corn research institute of Shandong Province Academy of Agricultural Sciences), combining 721 with Huang is paternal hybrid, and getting plumule is the hybridization F of purple
1For seed;
(2) plantation F
1In generation, is then with the hybridization F of purple plumule
1On behalf of female parent, be male parent with LX9801, backcross, gather in the crops the first backcross generation seed, choose the seed that plumule is a purple;
(3) choose the first backcross generation seed plantation that has purple embryo mark; Therefrom select good stress resistance, coordinate force is high and have a fine individual plant of purple embryo labels, continuously 5 generations of selfing; Plumule is the seed of purple among the selection offspring, cultivates the corn inbred line that the purple plumule is a purple " yellow comprehensive 801 ".
Embodiment 4 utilizes the purple embryo labels to identify the purity test of corn hybrid seed
2010 in Cangzhou, Hebei the beautiful industry experiment station of planting of deep blue; With corn inbred line Zheng 58 (Henan Academy of Agricultural Sciences's cultivation) is female parent; Pollen in the loose powder phase with Huang comprehensive 721 and Zheng 58 mixes, and gives Zheng 58 pollinations, and the results seed is 2436 during maturation; Randomly draw 300 and carry out the on-the-spot evaluation of naked eyes; Draw 165 of seeds that wherein have the purple plumule, not with 135 of the seeds of purple plumule, calculating hybrid rate according to the seed amount/formula of sample seed sum * 100% of purple plumule in corn hybrid seed purity (%)=sample is 55%; Then sample is identified with electrophoresis that drawing hybrid rate is 55%, qualification result is in full accord.Explain and utilize the purple plumule to identify corn seed purity accurately and reliably.
Claims (10)
1. corn that has the purple embryo labels, the plumule color that it is characterized in that said corn is a purple.
2. utilize the corn of the described purple embryo labels of claim 1 to carry out the method that purity of hybrid maize is identified, it is characterized in that with plumule being that colourless inbred line is a female parent, the inbred line that with the plumule is purple is a paternal hybrid, and results hybridization fruit ear mixes threshing; Randomly draw seed then, whether its plumule of naked-eye observation is purple, counts, and calculates the purity of corn hybrid seed then; The purity computing formula of corn hybrid seed is:
Band purple plumule seed number/sample seed sum * 100% in corn hybrid seed purity (%)=sample.
3. according to the described purity of hybrid maize authentication method of claim 2; It is characterized in that described plumule is that the corn inbred line of purple is yellowly to combine 721; Yellow comprehensive 721 belong to Zea corn kind (Zea maysL.); Be preserved in Chinese typical culture collection center on March 13rd, 2011, deposit number is: CCTCC No:P201104.
4. whether, according to plumule color identification seed on be this kind, if plumule is a purple, be exactly this kind if utilizing the purple embryo labels to carry out the method for corn variety authenticity identification, it is characterized in that for plumule being the kind of purple; If its plumule is not a purple, not this kind just.
5. the breeding method with the corn inbred line of purple embryo labels is characterized in that cultivating through cross breeding method or back cross breeding method.
6. according to the described breeding method of claim 5; It is characterized in that described cross breeding method, comprise that with the colourless inbred line of plumule be one of parent, is another parent's hybridization with the inbred line of being with the purple plumule; Carry out selfing then; Selecting the good and plumule of economical character among the gained self progeny is the seed of purple, carries out the selfing of 5～8 generations continuously and selects, and can obtain plumule is the new inbred line of purple.
7. according to the described breeding method of claim 5; It is characterized in that described back cross breeding method, comprise that with the colourless inbred line of plumule be recurrent parent, is non-recurrent parent with the inbred line of being with the purple plumule; Backcross; In backcross progeny, selecting the seed plumule is purple and other proterties material near recurrent parent, in 4～8 generations of continuous backcross, can obtain the new inbred line of purple plumule.
8. according to claim 5,6 or 7 described breeding methods, the inbred line that it is characterized in that described band purple plumule is yellow comprehensive 721.
9. the breeding method of the corn single cross hybrid of band purple embryo labels is characterized in that it being that corn inbred line with band purple plumule is a male parent, is that colourless corn inbred line is a hybridization of female parent with plumule, and the hybrid seed of gained is the corn single cross hybrid that plumule is a purple.
10. according to the described breeding method of claim 9, the inbred line that it is characterized in that described band purple plumule is yellow comprehensive 721.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110149085.6A CN102308747B (en) | 2010-06-25 | 2011-06-03 | Method for carrying out variety purity or trueness identification on corn by utilizing purple germ mark |
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|---|---|---|---|
| CN 201010209501 CN101897292A (en) | 2010-06-25 | 2010-06-25 | Breeding method of hybrid corn seeds with embryo labels |
| CN201010209501.2 | 2010-06-25 | ||
| CN201110149085.6A CN102308747B (en) | 2010-06-25 | 2011-06-03 | Method for carrying out variety purity or trueness identification on corn by utilizing purple germ mark |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106718857A (en) * | 2016-11-29 | 2017-05-31 | 云南瑞禾种业有限公司 | Efficient corn variety circulation selection |
| CN108362649A (en) * | 2017-12-18 | 2018-08-03 | 温州科技职业学院 | A method of the detection purity of hybrid of the color based on Cauliflower hypocotyl |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101897292A (en) * | 2010-06-25 | 2010-12-01 | 河北沧玉种业科技有限公司 | Breeding method of hybrid corn seeds with embryo labels |
| CN102972285B (en) * | 2012-12-05 | 2014-03-26 | 湖南杂交水稻研究中心 | Breeding method of hybrid crops |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1586156A (en) * | 2004-08-19 | 2005-03-02 | 浙江大学 | Breed selecting method for corn with leaf color mark |
| CN1843092A (en) * | 2006-05-18 | 2006-10-11 | 吉林省农业科学院 | Method for selecting and breeding corn new-bred through hybridized induction of unisexual seeding material |
| CN101897292A (en) * | 2010-06-25 | 2010-12-01 | 河北沧玉种业科技有限公司 | Breeding method of hybrid corn seeds with embryo labels |
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2010
- 2010-06-25 CN CN 201010209501 patent/CN101897292A/en active Pending
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- 2011-06-03 CN CN201110149085.6A patent/CN102308747B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1586156A (en) * | 2004-08-19 | 2005-03-02 | 浙江大学 | Breed selecting method for corn with leaf color mark |
| CN1843092A (en) * | 2006-05-18 | 2006-10-11 | 吉林省农业科学院 | Method for selecting and breeding corn new-bred through hybridized induction of unisexual seeding material |
| CN101897292A (en) * | 2010-06-25 | 2010-12-01 | 河北沧玉种业科技有限公司 | Breeding method of hybrid corn seeds with embryo labels |
Non-Patent Citations (1)
| Title |
|---|
| 张建成等: "玉米杂交种室内纯度的鉴定方法", 《种子世界》, vol. 25, no. 9, 31 December 1989 (1989-12-31), pages 25 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106718857A (en) * | 2016-11-29 | 2017-05-31 | 云南瑞禾种业有限公司 | Efficient corn variety circulation selection |
| CN108362649A (en) * | 2017-12-18 | 2018-08-03 | 温州科技职业学院 | A method of the detection purity of hybrid of the color based on Cauliflower hypocotyl |
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|---|---|
| CN101897292A (en) | 2010-12-01 |
| CN102308747B (en) | 2014-11-12 |
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