CN1994064A - A green waxy corn breeding method - Google Patents
A green waxy corn breeding method Download PDFInfo
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- CN1994064A CN1994064A CN 200610169640 CN200610169640A CN1994064A CN 1994064 A CN1994064 A CN 1994064A CN 200610169640 CN200610169640 CN 200610169640 CN 200610169640 A CN200610169640 A CN 200610169640A CN 1994064 A CN1994064 A CN 1994064A
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Abstract
The invention relates to a method for cultivating green corn self-cross system, wherein said method comprises that: crossing white corn and black corn; self crossing into violet corn, to be crossed with black corn or recycled; using yellow corn to pollinate, to obtain the green corn seed; self crossing into green corn self-cross system. Said invention also comprises the self-cross system between green corn self-cross system and white corn self-cross system to obtain the green corn self-cross system. The invention also provides a method for crossing green corn system that crossing the green corn self-cross systems, to obtain the cross system.
Description
Technical field
The invention belongs to the crop breeding technical field, specifically the present invention relates to the selection of a kind of green waxy corn inbred line and crossbreed.
Background technology
Waxy corn originates from China, be found at first in the southwest of China, it is the solids type corn producer sudden change by the locality plantation, through a kind of newtype that artificial selection and preserving is got off, it is subspecies (type) of corn, and its glutinous matter proterties is controlled by recessive gene site wx, seed is opaque, plant the skin tarnish, outward appearance is the wax shape, so claim waxy corn again.Its seed endosperm starch is 100% amylopectin, and is sticking soft and be rich in glutinous property after the boiling, is commonly called as glutinous corn.
By indoor chemical examination and attributional analysis, utilize the viewpoint of alimentology to analyze, glutinous beautiful No. 1 crude protein 10.03% of waxy corn new varieties west star of my company's seed selection, crude fat 4.86%, raw fiber 1.77%, thick content of starch is 69.92%, amylopectin is close to 100%, total energy 4149.84 kilocalorie/kilograms, and the total energy 3560 kilocalorie/kilograms of conventional corn exceed nearly 19.0%.In addition, after measured, calcium contains 0.05% in the mineral matter, is 4.17 times of conventional corn, and 101.4 milligrams/kilogram of iron are 2.03 times of conventional corn, and zinc is 69.06 milligrams/kilogram, is 3.69 times of conventional corn.Eating western star waxy corn is calcium, iron, zinc, the while tonic, in addition through browsing amount of literature data and facts have proved, waxy corn is under the effect of amylolytic enzyme, digestibility is 85%, and the digestibility of conventional corn only is 69%, calculating in other words like this, the digestibility of waxy corn has exceeded 23.2% than conventional corn, and in detecting various glutinous corn variety 18 seed amino acid analysis of components, being no lack of has the strain of high-lysine a lot, therefore, we can say that waxy corn has high nutritive value.
For the color of waxy corn, we have also carried out deep exploration, combine discussion, demonstration with the theory of yin-yang and five elements (metal, wood, water, fire and earth), the tcm theory of traditional Chinese culture.Traditional Chinese medicine thinks that the people has the five internal organs (liver, the heart, spleen, lung, kidney), six internal organs＜courage, small intestine, stomach, large intestine, bladder, three warmers (upper, middle and lower), and the people has face (order, tongue, mouth, nose, ear), the seven apertures (binocular, ears, amphirhinal, flatly).In the natural food five colors are arranged, promptly blue or green, red, yellow, white, black, the thing that people ate also has the five tastes, sour, bitter, sweet, peppery, salty or the like.Five color and five flavours and human organ corresponding relation, cyan is gone into liver, and redness of the skin or complexion is gone into the heart, and yellow is gone into taste, and white is gone into lung, and black is gone into kidney.In the five tastes, tart flavour is gone into liver, and bitter taste is gone into the heart, and sugariness is gone into spleen, and hot taste is gone into lung, and saline taste is gone into kidney.According to textual criticism, " going into " person is the effect of taking a tonic or nourishing food to build up one's health health care, improving the health.Motherland's traditional Chinese medicine is exactly according to the dialectical relationship of yin-yang and five elements and human five internal organs's six internal organs and property, look, the flavor of Chinese herbal medicine, has formed complete theory of traditional Chinese medical science.So the different colours crlutinous maize has certain nutrition health-care functions to viscera,, will expand the frontier that waxy corn is used with the main direction of the motley seed color of seed selection as glutinous corn breeding.
Learn theory and practice according to modern breeding, we with artistical gimmick and eye, mould Chinese multicolored waxy corn to the traditional theory of TCM of China, the organic combination of alimentology viewpoint, genetics and morphology are combined, thinking in images and logical thinking are combined.By the quick process of breeding theory and practice, we have enriched the theory of maize genetic thremmatology in the technological means and the application in practice innovation aspect of multicolored glutinous corn breeding, create the waxy corn of different colours.Specifically, we have selected white, yellow, redness, black, purple waxy corn, the existing at home report of the waxy corn of these colors; Simultaneously, we find, after white waxy corn and the hybridization of black waxy corn, among its offspring except that black and white two looks are arranged, also have a lot of middle colors, as light black, hyacinthine etc., it is hyperchromic to utilize the seed of colors in the middle of these to carry out through the means of backcrossing or hybridize, and we successfully select the seed color and are green waxy corn.
Up to the present, at home, abroad, be the method for the crossbreeded glutiuous maize of green for green waxy corn inbred line and seed color about selecting the seed color, do not appear in the newspapers as yet.
Summary of the invention
The objective of the invention is to utilize existing germ plasm resource, by genetic recombination, utilize hybridize, backcrosses, selfing, separation select, add for adding generation in conjunction with Hainan Island spring and summer in winter in the north, carried out 1 year three generations transformation green waxy corn process fast; Through germplasm innovation, select high-combining ability, economical character is good, genotype is isozygotied green waxy corn inbred line.
The invention provides a kind of selection of green waxy corn inbred line, specifically comprise the steps:
1) with white waxy corn and the hybridization of black waxy corn, obtains crossbreed;
2) its crossbreed is carried out selfing and isozygoty, select the purple waxy corn;
3) with the hybridization of above-mentioned selected purple waxy corn and black waxy corn or backcross;
4) its hybridization or first backcross generation are pollinated to it with yellow waxy corn pollen, obtain the green waxy corn seed;
5) above-mentioned green waxy corn seed is carried out selfing and isozygoty, obtain the green waxy corn inbred line of proterties stable and consistent.
The selection of a kind of green waxy corn inbred line provided by the invention, also comprise with above-mentioned selected not consanguine green waxy corn inbred line of educating and white waxy corn inbred line and hybridizing, carry out 4-10 again and isozygoty for selfing and select the green waxy corn inbred line.
The present invention also provides the selection of a kind of green waxy corn crossbreed, hybridizes with above-mentioned selected not consanguine green waxy corn inbred line of educating, and selects the green waxy corn crossbreed.
Wherein said white waxy corn is selected from: glutinous beautiful No. 1 of BN101, BN201, DHN13B4 or western star.
Wherein said black waxy corn is selected from: Lay is black 12, black glutinous No. 1 of Xuzhou black glutinous, EN12-5, EN951-6 or western star.
Wherein said yellow waxy corn is selected from: HN8972-2-2, HN196-3, Xi Xinghuang glutinous No. 6 or DHN12.
Step 2 wherein) described selfing is 4-10 generation; The described selfing of step 5) is 4-10 generation.
Related biomaterial BN101, BN201, DHN13B4, EN12-5, EN951-6, HN8972-2-2, HN196-3 and DHN12 among the present invention is the breeding material of being cultivated by our company oneself; Yellow glutinous No. 6 of glutinous beautiful No. 1 of the star in west, western star black glutinous No. 1 and western star are the crossbreeded glutiuous maizes by our company oneself cultivation; Lay black 12 and Xuzhou are black glutinous, are landrace.
Above-mentioned biomaterial was all gone up open at " new variety of agricultural plant protection communique " before the applying date.Wherein BN101, BN201 and western star glutinous beautiful No. 1 " new variety of agricultural plant protection communique " 2000, the 1st phase, the 7-8 page or leaf is open; DHN13B4 was " new variety of agricultural plant protection communique " 2006, and the 3rd phase, the 22nd page open; Black glutinous No. 1 of EN12-5, EN951-6, western star, Lay black 12 and Xuzhou are black glutinous, and " new variety of agricultural plant protection communique " 2004, the 2nd phase, the 22nd page open; Yellow glutinous No. 6 of HN8972-2-2, HN196-3 and western star, " new variety of agricultural plant protection communique " 2006, the 2nd phase, the 15th page was open; DHN12 was " new variety of agricultural plant protection communique " 2006, and the 2nd phase, the 16th page open.
Above-mentioned biomaterial is kept at applicant Shandong Denghai Seeds Co.,Ltd at present, and the applicant guarantees to provide to the public in 20 years present patent application days.
Advantage of the present invention and good effect are:
The present invention carries out genetic recombination by utilizing existing germ plasm resource, and the corn kernel color has been carried out more deep research, has broken through Mendel's law on technical know-how, is a kind of technological invention and innovation; This technology has the leading level in the world, has filled up the blank that the home and abroad does not have seed selection green waxy corn inbred line and green waxy corn crossbreed, has very high theory and is worth and using value.
Description of drawings
The breeding line spectrogram of Fig. 1 green waxy corn inbred line
Embodiment
Following examples are used to illustrate the present invention, but are not used for limiting the scope of the invention.
The seed selection process of the green N3-222 of embodiment 1 green waxy corn inbred line, green N3-212, green N3-2132
In December, 1997: to the west of the glutinous beautiful No. 1 maternal BN101 of star be maternal, be that male parent is hybridized with Lay black 12, composition basic material F
1
Year November in March, 1998-2000: to above-mentioned basic material F
1Carry out the selfing of 6 generations with the breeding process in annual 2 generations and isozygoty, obtain stable purple waxy corn inbred line ZN12.
In December, 2000: ZN12 is a female parent with purple waxy corn inbred line, is that male parent is hybridized with EN12-5, gets F
1
June calendar year 2001: plantation F
1Plant 100 strains, using the pollen of yellow corn HN8972-2-2 (female parent that Xi Xinghuang is glutinous No. 6) is its pollination.
October calendar year 2001: obtain color and be 18 of green corn kernels.
Year February in November calendar year 2001-2005: carry out selfing with the breeding process in annual 3 generations and isozygoty, obtain 3 stable green waxy corn inbred lines through 10 generations of selfing continuously, wherein the selfing of grass green waxy corn is green N3-222; 2 of bottle green waxy corn inbred lines are divided into green N3-212 and green N3-2132.
The seed selection process of the green N222 of embodiment 2 green waxy corn inbred lines
In December, 1997: to the west of the glutinous beautiful No. 1 male parent BN201 of star be maternal, with Xuzhou black glutinous be that male parent is hybridized, composition basic material F
1
Year February in March, 1998-2000: to above-mentioned basic material F
1Carry out the selfing of 6 generations with the breeding process in annual 3 generations and isozygoty, obtain stable purple waxy corn inbred line ZN951.
In March, 2000: ZN951 is a female parent with purple waxy corn inbred line, is that male parent is hybridized with EN951-6, gets F
1
In July, 2000: plantation F
1Plant 100 strains, using the pollen of yellow corn HN196-3 (male parent that Xi Xinghuang is glutinous No. 6) is its pollination.
In October, 2000: obtain color and be 10 of green corn kernels.
Year October in November, 2000-2002: carry out selfing with the breeding process in annual 3 generations and isozygoty, obtain the stable green N222 of green waxy corn inbred line through 6 generations of selfing continuously.
The seed selection process of the green N142 of embodiment 3 green waxy corn inbred lines
In November, 2000: the purple waxy corn inbred line ZN951 with embodiment 2 gained is a female parent, is that male parent is hybridized with EN951-6, gets F
1
June calendar year 2001: plantation F
1Plant 100 strains, using the pollen of yellow corn DHN12 is its pollination.
October calendar year 2001: obtain color and be 10 of green corn kernels.
Year February in November calendar year 2001-2005: carry out selfing with the breeding process in annual 3 generations and isozygoty, obtain the stable green N142 of green waxy corn inbred line through 10 generations of selfing continuously.
The seed selection process of the green N13B4 of embodiment 4 green waxy corn inbred lines
In November, 2002: with green N222 is female parent, is that male parent is formed basic material F with white waxy corn inbred line DHN13B4 (male parent that Xi Xingbai is glutinous No. 13)
1
Year October in March, 2003-2005: to F
1Carry out continuous selfing 8 generations of isozygotying and bred green N13B4 inbred line.
The seed selection process of the long green N25 of embodiment 5 green waxy corn inbred lines
January calendar year 2001: the purple waxy corn inbred line ZN12 with embodiment 1 gained is a female parent, is that male parent is hybridized with EN951-6, breeds glutinous No. 1 crossbreed of Xi Xingqing.
June calendar year 2001: to the west of star blue or green glutinous No. 1 (white endosperm, seed are the secondary colour of black purple blueness) for maternal, to the west of star yellow glutinous No. 6 (yellow endosperm) hybridize 20 strains for male parent, to wait to see the seed variation.
October calendar year 2001: on 4 fruit ears of results, green waxy corn seed occurred.
November calendar year 2001: the green seed monoseeding that will obtain in Mei Shan base, Sanya, Hainan carries out selfing, singly selects green seed material in support.
Year October in June, 2002-2005: carry out selfing with the breeding in annual 3 generations and isozygoty, obtain the long green N25 of stable green waxy corn inbred line through 10 generations of continuous selfing.
The seed selection process of the green N61 of embodiment 6 green waxy corn inbred lines
In December, 1999: to the west of star glutinous beautiful be for No. 1 maternal, be that male parent is hybridized with EN12-5, form basic material F
1
In March, 2000-November calendar year 2001: to above-mentioned basic material F
1Carry out the selfing of 4 generations with the breeding process in annual 2 generations and isozygoty, obtain stable purple waxy corn inbred line ZN118.
December calendar year 2001: ZN118 is a female parent with purple waxy corn inbred line, is that male parent is backcrossed with EN12-5, gets BC
1
In June, 2002: plantation BC
1Plant 100 strains are its pollination with yellow corn west yellow glutinous No. 6 pollen of star.
In October, 2002: obtain color and be 33 of green corn kernels.
Year February in November, 2002-2006: carry out selfing with the breeding process in annual 3 generations and isozygoty, obtain the stable green N61 of green waxy corn inbred line through 10 generations of selfing continuously.
The seed selection process of the green N133 of embodiment 7 green waxy corn inbred lines
In December, 1998: to the west of star glutinous beautiful be for No. 1 maternal, to the west of star hybridize composition basic material F for male parent black glutinous No. 1
1
Year June in March, 1999-2002: to above-mentioned basic material F
1Carry out the selfing of 10 generations with the breeding process in annual 3 generations and isozygoty, obtain stable purple waxy corn inbred line ZN111.
In July, 2002: ZN111 is a female parent with purple waxy corn inbred line, to the west of star backcross for male parent for black glutinous No. 1, must BC
1
In December, 2002: plantation BC
1Plant 100 strains, using the pollen of yellow corn inbred line HN8972-2-2 is its pollination.
In February, 2003: obtain color and be 22 of green corn kernels.
Year June in March, 2003-2004: carry out selfing with the breeding process in annual 3 generations and isozygoty, obtain the stable green N133 of green waxy corn inbred line through 4 generations of selfing continuously.
The seed selection process of embodiment 8 green waxy corn crossbreed " green emerald "
Year March in November, 2005-2006: with green N3-222 is maternal, obtains " green emerald-1 " with green 13B4 hybridization; With green N3-2132 is maternal, obtains " green emerald-2 " with green N142 hybridization; With green N3-212 is maternal, obtains " green emerald-3 " with green N222 hybridization.
Claims (8)
1, a kind of selection of green waxy corn inbred line is characterized in that, comprises the steps:
1) with white waxy corn and the hybridization of black waxy corn, obtains crossbreed;
2) its crossbreed is carried out selfing and isozygoty, select the purple waxy corn;
3) with the hybridization of above-mentioned selected purple waxy corn and black waxy corn or backcross;
4) its hybridization or first backcross generation are pollinated to it with yellow waxy corn pollen, obtain the green waxy corn seed;
5) above-mentioned green waxy corn seed is carried out selfing and isozygoty, obtain the green waxy corn inbred line of proterties stable and consistent.
2, the method for claim 1 is characterized in that, this method also comprises with above-mentioned selected not consanguine green waxy corn inbred line of educating and white waxy corn inbred line and hybridizing, and carries out 4-10 again and isozygotys for selfing and select the green waxy corn inbred line.
3, the method for claim 1 is characterized in that described white waxy corn is selected from: glutinous beautiful No. 1 of BN101, BN201, DHN13B4 or western star.
4, the method for claim 1 is characterized in that described black waxy corn is selected from: Lay is black 12, black glutinous No. 1 of Xuzhou black glutinous, EN12-5, EN951-6 or western star.
5, the method for claim 1 is characterized in that described yellow waxy corn is selected from: HN8972-2-2, HN196-3, Xi Xinghuang glutinous No. 6 or DHN12.
6, the method for claim 1 is characterized in that described step 2) described selfing is 4-10 generation.
7, the method for claim 1 is characterized in that the described selfing of described step 5) is 4-10 generation.
8, a kind of method by the described method seed selection of claim 1-7 green waxy corn crossbreed is characterized in that the not consanguine green waxy corn inbred line of seed selection is hybridized, and selects the green waxy corn crossbreed.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102283095A (en) * | 2011-06-22 | 2011-12-21 | 云南省农业科学院粮食作物研究所 | Method for breeding hybrid seeds of color waxy corn |
| CN102870668A (en) * | 2012-10-11 | 2013-01-16 | 南京市蔬菜科学研究所 | Selective breeding method for black glutinous maize inbred line |
| CN105028175A (en) * | 2015-06-03 | 2015-11-11 | 南京市蔬菜科学研究所 | Breeding method of selfing lines of waxy corns and white waxy corns |
| CN106962178A (en) * | 2017-04-26 | 2017-07-21 | 重庆市农业科学院 | A kind of selection of black waxy corn cenospecies |
-
2006
- 2006-12-26 CN CNB2006101696400A patent/CN100486424C/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102283095A (en) * | 2011-06-22 | 2011-12-21 | 云南省农业科学院粮食作物研究所 | Method for breeding hybrid seeds of color waxy corn |
| CN102870668A (en) * | 2012-10-11 | 2013-01-16 | 南京市蔬菜科学研究所 | Selective breeding method for black glutinous maize inbred line |
| CN105028175A (en) * | 2015-06-03 | 2015-11-11 | 南京市蔬菜科学研究所 | Breeding method of selfing lines of waxy corns and white waxy corns |
| CN105028175B (en) * | 2015-06-03 | 2018-03-09 | 南京市蔬菜科学研究所 | A kind of selection of waxy corn and white Waxy Corn Inbred Lines |
| CN106962178A (en) * | 2017-04-26 | 2017-07-21 | 重庆市农业科学院 | A kind of selection of black waxy corn cenospecies |
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| CN100486424C (en) | 2009-05-13 |
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