CN103975846A - Novel method for creating dual-purpose rice genic male sterile line - Google Patents
Novel method for creating dual-purpose rice genic male sterile line Download PDFInfo
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- CN103975846A CN103975846A CN201410205500.9A CN201410205500A CN103975846A CN 103975846 A CN103975846 A CN 103975846A CN 201410205500 A CN201410205500 A CN 201410205500A CN 103975846 A CN103975846 A CN 103975846A
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Abstract
The invention provides a novel method for creating a dual-purpose rice genic male sterile line. The method comprises the following steps: conducting sexual hybridization or backcross by selecting sensitive rice variety as a female parent and a sensitive rice variety as a male parent (or matching vice visa), separating photo-sensitive and temperature-sensitive dual-purpose genic male sterile plants from the offspring F2-F6 generations, carrying out six to eight generations of pedigree selection to culture the genic male sterile line. The sterile plants separated from the F2 generation are identified under different natural photo and temperature conditions, the long photo-sensitive and temperature-sensitive dual-purpose genic male sterile line of rice can be obtained under the photo-temperature conditions of more than 12 hours of natural illumination, and short photo-sensitive and temperature-sensitive dual-purpose genic male sterile line of the rice can be obtained under the photo-temperature conditions of more than 12 hours of natural illumination. By using the creating method, the applications of the dual-purpose genic male sterile line in production can be enriched, the source range of the genic male sterile line in production can be widened, the photo-sensitive and temperature-sensitive dual-purpose rice genic male sterile line for production demand can be created, and the method has the characteristics of being simple and convenient, practical, and good in effect.
Description
Technical field
The invention belongs to rice breeding technology field, relate in particular to the initiative new method of a kind of paddy rice light, temperature sensitive dual purpose genic male sterile line.
Background technology
Within 1973, the bright pine of stone has been found 3 strain nature male sterile plants (Nongken 58S) the land-reclaimable 58 large Tanakas of Hubei Mian Yangxian (existing Xiantao City) Shahu Lake seed farm round-grained rice in evening, finds that they have the characteristic that can educate long day sterile, short day subsequently by the research unit such as Hubei Prov. Acdemy of Agricutural Sciences, Wuhan University by natural interval sowing." one is dual-purpose " that Shi Mingsong proposed based on this male sterile characteristic in 1981 to grow the production of hybrid seeds under day high temperature, within short day, under low temperature, breed utilizes heterotic imagination.Pulled open the prelude of China's two-line hybrid rice development in an all-round way for this reason.
Go through 30 years of researches, China has selected large quantities of by the paddy rice dual-purpose caryon sterile line of provincial-level appraisal or authorization, and configures many strong advantage combinations (kind) with production practical value.By 2007, the hybrid combination large-area applications of existing 78 dual purpose genic male sterile lines preparation, in production, combined totally 243 by the double-linear hybrid rice of provincial or national authorization.In recent years, the popularizing area of two-line hybrid rice constantly rises, and accounts for 30～40% of hybrid rice sowing area.Two-line hybrid rice newly combines, the integrated innovation of new technology and applying, for China's grain " nine connect increase " has played important support effect.
So far, dual purpose genic male sterile line temperature influence is larger, is subject to the impact of illumination less, so the dual purpose genic male sterile line of producing at present upper application is low-temperature-sensitive caryon sterile line mostly, is secondly interaction by light and temperature caryon sterile line, photosensitive-sensitive sterile line is few.Therefore, in two-line hybrid rice is produced, dual purpose genic male sterile line breeding and the production of hybrid seeds have particular/special requirement to temperature, otherwise, because the educated temperature range of caryon sterile line is very narrow, fertility-sensitive period is easy to run into exceed can educate the abnormal high temperature of temperature range or low temperature and cause reproductive failure, causes larger economic loss to seed manufacturing enterprise.Moreover, these dual purpose genic male sterile lines on producing in application process because fertility drift and seed production technology are left to be desired, cause low-temperature-sensitive caryon sterile line origin temp progressively to raise, badly influence purity and the production of hybrid seeds safety of double-line hybrid rice, even cause production of hybrid seeds failure.
Above-mentioned situation, urgent need solution paddy rice dual-purpose caryon sterile line is subject to the problem of low temperature effect in the time of the production of hybrid seeds.The male sterile line of applying in existing production is the hybridization transformation offspring who derives from " Nongken 58S " mostly, and small part is the dual purpose genic male sterile line (as peace agriculture S) in new source.Therefore, in Rice Production, the caryon sterile line source of large-area applications is narrow at present, for avoiding the generation potential threat that is very popular of damage by disease and insect, be starved of the dual purpose genic male sterile line of applying in the current production of continuous improvement, not only to find new core sterile material, and extremely be necessary to find the new method of dual purpose genic male sterile line initiative.
Summary of the invention
The object of the present invention is to provide the initiative new method of a kind of paddy rice light, temperature sensitive dual purpose genic male sterile line, be intended to solve paddy rice dual-purpose caryon sterile line and be subject to the problem of low temperature effect in the time of the production of hybrid seeds;
The present invention realizes like this, the initiative new method of a kind of paddy rice light, temperature sensitive dual purpose genic male sterile line, comprise the following steps: taking sensitization rice varieties as maternal, temperature-sensitive rice varieties is that male parent (also can opposite way) is carried out sexual hybridization or backcrosses, from F2～F6 generation, separate bright dipping, the sterile strain of temperature sensitive dual-purpose core, be commissioned to train and bring out stable dual purpose genic male sterile line through pedigree selection 6～8.In the isolated sterile strain of F2 generation, be allowed to condition at and do not share the same light, identify under warm natural conditions, can obtain paddy rice light, temperature sensitive dual purpose genic male sterile line.
The present invention overcomes the deficiencies in the prior art, the new method of a kind of paddy rice light, the initiative of temperature sensitive dual purpose genic male sterile line is provided, it is maternal selecting sensitization rice varieties, temperature-sensitive rice varieties is that male parent is carried out sexual hybridization or backcrosses, from F2～F6 generation, separate bright dipping, the sterile strain of temperature sensitive dual-purpose core, be commissioned to train and bring out stable dual purpose genic male sterile line through pedigree selection 6～8.In the isolated sterile strain of F2 generation, be allowed to condition at and do not share the same light, identify under warm natural conditions, obtain paddy rice light, temperature sensitive dual purpose genic male sterile line.The present invention formulates new method will widen the above caryon sterile line source scope of producing, and the dual purpose genic male sterile line of the upper demand of solution production, has easy, practical, the effective feature of method.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearer, below in conjunction with embodiment, the present invention is further elaborated.Should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not intended to limit the present invention.
Embodiment 1
Select sensitization Dongxiang Wild Rice and japonica rice hybridization, backcross progeny, through the pedigree seed selection of 10 generations, be bred as the sensitization inclined to one side japonica rice strain of Xian in evening " eastern wild offspring-14 ", inclined to one side Xian upland rice incross by the sensitization inclined to one side japonica rice strain of Xian in evening " eastern wild offspring-14 " with Liaoning academy of agricultural sciences, make its offspring accelerate to stablize, then Sanya, Hainan rattan bridge winter breeding plant F4 for colony in the sterile strain of seed selection 6 strain core (Hainan numbering, 25456-1,25456-2,25457-1,25461,25687-1,25690-1), these sterile strains are in Hainan fertility performance sterility.This 6 strain is taken back to Fertility identification under the natural conditions of Nanchang, and the performance that result has is sterile, and some fertile plants illustrate that sterile strain shows as the quick caryon sterile line type of long light (temperature), otherwise are the quick caryon sterile line type of short light (temperature).
Embodiment 2
Select sensitization Dongxiang Wild Rice and restorer R664 hybridization, backcross once by temperature-sensitive R664 restorer again, selfing three generations (B1F3).Go to Sanya, Hainan rattan bridge winter breeding to plant (B1F4), 3 (Hainan numberings of the sterile strain of seed selection from colony, 25800-1,25802-1,25802-2), 3 standing grain root and stem of certain plants are taken back to Nanchang and observe fertility, the sterile strain of 25802-1 is solid in heading on June 28 loose powder, and heading on July 5 loose powder is solid; Heading on the sterile strain of 25800-1 July 15 loose powder is solid, and the sterile strain of 25802-2 is that heading on July 18 loose powder is solid, and self-fruitful rate 68～82% is shown to be the type of the quick type caryon sterile line of short light (temperature).
Embodiment 3
Select the wild wild filial generation in sensitization Dongxiang autumn in Nanchang---14/ upland rice (introduction of Liaoning academy of agricultural sciences) hybridization obtains F1 generation, in the sowing on December 5 of Hainan plantation F1 generation, maturation was mixed to receive and was obtained F2 generation mid-April, sow in Nanchang one season seed rice plant F2 generation, in colony, select individual plant to obtain F3 generation, Hainan adds generation then, sow for November 28, heading on March 22nd, 2013, April 23 maturation, find to have 3 sterile strains in F3 colony, wherein fertility situation is observed in the plantation of 2 Zhu Wahui Nanchang solariums, result is that heading performance on July 1 is sterile, July 5, 10 days, 15 days, until October 1 is sterile, October 4 transferred to and can educate, show as the type of the quick caryon sterile line of long light (temperature).
Embodiment 4
Select the short light sensitive nuclear sterility of paddy rice (Yichuan Nong Zhuan institute provides) through how for directive breeding, having obtained short light-sensitive male sterility rice should DS1 (302).Make female parent by " red round-grained rice " (clever sharp purple is dark, shell look yellow) temperature-sensitive kind, carry out sexual hybridization, backcross for male parent with B3 (sensitization rice variety in evening), separate progeny selection and go out short photosensitive-sensitive sterile line.This male sterile line (suitable DS1) is under Sanya, Hainan nature short-day (every day is below 12 hours), temperature (average below 30 DEG C) condition, show as male sterile, under nature long-day (every day is more than 12 hours), high temperature (the average more than 30 DEG C) condition of Jiangxi, show as male-fertile.
Embodiment 5
Select sensitization Restoring Line of Japonica Rice C418 and temperature-sensitive Xian restorer morning 102 to hybridize, select the quick caryon sterile line 37S of long light (temperature) from separating Offspring F2 generation.Sow at the beginning of 12 months in Hainan, heading performance male sterile mid-March, the sterile phase is more than 30 days; Its rice root and stem of certain plants is taken back Nanchang plantation, heading performance male-fertile, ripening rate 78.8%.
Embodiment 6
It is maternal selecting sensitization Dongxiang Wild Rice (late rice type), (two or nine short No. 7/capital rice 66) is male parent, F1 generation radiation, seed selection becomes japonica rice strain, is difficult for shattering, photosensitivity is strong, with " No. 1, bamboo section " hybridization, separating the individual plant that 37 grams of thousand kernel weight have appearred in offspring again, there is sterile strain (B1S) in nineteen ninety offspring, go to Hainan adding generation qualification in the winter in November, show as male-fertile.Do not share the same light, field plot test under warm natural conditions, B1S is the quick caryon sterile line type of long light (temperature).
Compare and the shortcoming and defect of prior art, the present invention has following beneficial effect: the initiative new method of paddy rice light of the present invention, temperature sensitive dual purpose genic male sterile line is easy, practical, effective.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any amendments of doing within the spirit and principles in the present invention, be equal to and replace and improvement etc., within all should being included in protection scope of the present invention.
Claims (1)
1. a paddy rice dual-purpose nuclear sterile rice initiative new method, it is characterized in that comprising the following steps: taking sensitization rice varieties as maternal, temperature-sensitive rice varieties is that male parent (also can opposite way) is carried out sexual hybridization or backcrosses, in its Offspring F2～F6 generation, separate bright dipping, the sterile strain of temperature sensitive dual-purpose core, be commissioned to train and bring out stable caryon sterile line through pedigree selection 6～8; Be allowed to condition in the isolated sterile strain of F2 generation and do not share the same light, identify under warm natural conditions, can obtain the long light of paddy rice, temperature sensitive or short light, temperature sensitive dual purpose genic male sterile line.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109042304A (en) * | 2018-09-04 | 2018-12-21 | 江苏里下河地区农业科学研究所 | The selection of blast resisting good quality and high output two-line hybrid rice combination |
| CN115968776A (en) * | 2023-03-20 | 2023-04-18 | 云南省农业科学院粮食作物研究所 | Wide-compatibility dual-purpose genic male sterile line breeding method for low fertility starting point temperature of rice and application |
Citations (4)
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| JPH02207722A (en) * | 1989-02-03 | 1990-08-17 | Nogyo Kenkyu Center Shocho | Production of f1 hybrid of rice plant by utilizing temperature-sensitive nuclear gene male fertility |
| CN1115596A (en) * | 1993-10-21 | 1996-01-31 | 中国科学院昆明植物研究所 | Breeding method of reversible dual-purpose male nucleus sterile line of rice |
| WO2001006845A1 (en) * | 1999-07-25 | 2001-02-01 | State Of Israel/Ministry Of Agriculture | A method for obtaining 100 % male sterile plants to serve as the female parent in hybrid seeds production |
| CN102599044B (en) * | 2012-03-14 | 2013-05-15 | 安徽省农业科学院水稻研究所 | Breeding method of wide-compatibility photo-thermo sensitive sterile rice pyramiding lines |
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2014
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Patent Citations (4)
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| JPH02207722A (en) * | 1989-02-03 | 1990-08-17 | Nogyo Kenkyu Center Shocho | Production of f1 hybrid of rice plant by utilizing temperature-sensitive nuclear gene male fertility |
| CN1115596A (en) * | 1993-10-21 | 1996-01-31 | 中国科学院昆明植物研究所 | Breeding method of reversible dual-purpose male nucleus sterile line of rice |
| WO2001006845A1 (en) * | 1999-07-25 | 2001-02-01 | State Of Israel/Ministry Of Agriculture | A method for obtaining 100 % male sterile plants to serve as the female parent in hybrid seeds production |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109042304A (en) * | 2018-09-04 | 2018-12-21 | 江苏里下河地区农业科学研究所 | The selection of blast resisting good quality and high output two-line hybrid rice combination |
| CN115968776A (en) * | 2023-03-20 | 2023-04-18 | 云南省农业科学院粮食作物研究所 | Wide-compatibility dual-purpose genic male sterile line breeding method for low fertility starting point temperature of rice and application |
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Application publication date: 20140813 |