CN1115596A - Breeding method of reversible dual-purpose male nucleus sterile line of rice - Google Patents
Breeding method of reversible dual-purpose male nucleus sterile line of rice Download PDFInfo
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Abstract
In the present invention, water rice or dry rice breeds with large ecological difference in illumination and temp. are selected as parents, which are passed through hybridization, discrimination, selection, continuous single-plant selection and stabilization to breed stable sterile line. The sterile line bred has stable sterility over 99.9%, recuperable selfed seeding rate up to 50-90%, recuperable top-cross breeds up to 75%, wide mating property and wide suitable range. Therefore, the present invention provides excellent material for heterosis combination and disease-resistant breeding.
Description
The present invention relates to field of agricultural science and technology semilate rice class heterosis utilization category, relate in particular to the selection of reversible dual-purpose male nucleus sterile line of rice and the reversible dual-purpose male nucleus sterile line of rice material that obtains according to this method.
The research of rice class dual purpose genic male sterile line starts from 1973, Hubei Mr. Shi Mingsong has found " land-reclaimable 58S " dual purpose genic male sterile line first, be to belong to sub-sterile type of high temperature long-day, after this, found " pacifying agricultural S " male sterile line again in succession with several different kytoplasm types such as " agricultural S weigh ", but some novel male sterile lines that still form from " land-reclaimable 58S " transformation that great majority are applied to produce.The cytoplasm of these male sterile lines is still from " land-reclaimable 58S ", the abortion type is similar to " land-reclaimable 58S ", still exist fertility instability, shortcomings such as advantage is not too strong, increase very big risk to large scale application, and, do not see so far in the prior art the ripe intactly technology of seed selection rice class male sterility line of a cover is arranged, most seed selection male sterile lines are all found or are transformed male sterile line from natural world and get, or by hybridization discovery sterile material, but can not receive seed can't utilize, perhaps method poor repeatability, the male sterile line instability does not also have a kind of ripe breeding technique that can subjectively formulate out the male sterile line with certain proterties or several proterties according to the human needs.The technology of existing seed selection male sterile line exists the shortcoming of aspects such as seed selection progress, seed selection quantity, cytoplasm type be restricted, does not have so far a kind ofly to utilize artificial hybridization to combine with methods such as regeneration to obtain the selection of the original non-existent reversible dual-purpose male nucleus sterile line of rice of natural world.
The object of the present invention is to provide a kind of employing artificial hybridization and renovation process combination, find sterile material with artificial hybridization, allow its regeneration, through differentiating, screening, individual plant is selected continuously, stabilizing step is bred stable, the method of the former non-existent valuable various reversible dual-purpose male nucleus sterile line of rice materials of natural world, thereby the realization mankind can select for use the different parents with various various traits to come combo according to different demands and select various good paddy rice or upland rice male sterile line, for the heterotic utilization of rice class provides a kind of stable, the new method that the seed selection step is easy.
The present invention also aims to provide the source of cytoplasm that obtains according to selection of the present invention abundant good stable reversible dual-purpose male nucleus sterile line of rice material, thereby be that bright prospects are opened up in seed selection and the application that paddy rice, upland rice two are.
To achieve these goals, the invention provides following technical proposals:
Breed the reversible dual-purpose male nucleus sterile line of rice material by steps such as hybridizing, differentiate, screen, continuous individual plant is selected, stablized, said hybridization is to select for use big paddy rice of light temperature ecocline or upland rice variety to carry out artificial hybridization as the parent, and said discriminating is with the F after the hybridization
1In generation, made rice culture early, chooses the fertile flower powder and the pollen sterile ratio is regenerated near 1: 1 combination, and said screening is to choose ratoon rice or summer the fertile flower powder reaches more than 90% during rice culture, self-fruitful rate is near the F of conventional rice variety
2In generation,, said continuous individual plant is selected and be stable was from above-mentioned F in the cultivation in early spring
3In the sterile strain that generation separates, select short partially, the comprehensive proterties of plant height individual plant good, that the pollen abortion degree is high let alone to regenerate solid, so repeat to select 7 to 8 generations or more advanced lines then breed stable reversible dual-purpose male nucleus sterile line of rice.
The parent selects one to be strong water, the upland rice variety of photosensitivity in the said method crossover process, and another is the strong water of response to temperature, upland rice variety.
With F described in the said method
2It is spring of 20-25 ℃ that generation is planted in daily mean temperature with colony.
Described cross method adopts direct method, three friendship methods or backcross method to let alone a kind of.
The present invention provides the reversible dual-purpose male nucleus sterile line of rice material that obtains according to above-mentioned selection simultaneously, described sterile line material is the ABA type, its fertility changes with the change of light and temperature condition, under low temperature short-day condition, be sterile, recover self-fertility under the high temperature long-day condition, transfer to sterilely again, the fertility switching mechanism is a light temperature complementation.
Above-mentioned each sterile line material, the light temperature value that fertility changes is 13h/24-26 ℃, the sterile phase is 1 to 3 months.
The sterile degree of above-mentioned sterile line material reaches more than 99.9%, and sterile plant rate is up to 100% because of homophyletic system is not different.
Above-mentioned sterile line material, the recovery source is wide, and 75% test cross kind all can have the solid ability of higher recovery to male sterile line, and the highest recovery ripening rate can reach more than 90%.
Above-mentioned sterile line material, pollen abortion approach are nucleosis type and nuclear involution form.
Pollen abortion is thorough at meiosis telophase I period for the pollen abortion of wherein nucleosis type, and the abortion degree reaches 99-100%.
Above-mentioned sterile line material is divided into indica cms line, the sterile type of round-grained rice type and Xian round-grained rice osculant male sterile line.Wherein indica cms line shows strong advantage, the restoring degree height with morning behind the rice hybridization.Japonica rice sterile line cooperates advantage strong with long-grained nonglutinous rice or upland rice, and is bad slightly with the japonica rice heterosis, hybrid vigor.Xian round-grained rice osculant male sterile line cooperates advantage strong with long-grained nonglutinous rice, and disease resistance is strong, and drought resistance is strong.
The fertility switching mechanism of above-mentioned sterile line material is meant that the Fertility Expression of described male sterile line is subjected to the dual control of temperature and illumination, i.e. light temperature complementation, and when male sterile line is in the photaesthesia temperature range when following, illumination length is inoperative; When male sterile line is in the photaesthesia temperature range when above, illumination length plays important compensatory effect.Light temperature complementation formula S=n (t
21)-k represents, in the formula, S=fertile flower powder or ripening rate, the t=Celsius temperature, 1=illumination hour length, the n=coefficient, the k=constant, sterile degree result according to each male sterile line records under several different light and temperature conditions makes regression figure, finds out the average temperature value intermediate value when male sterile line is sterile fully can educate with recovery again in regression figure, the substitution formula, calculate temperature intermediate value and the critical value of each male sterile line under any illumination length condition, calculate each male sterile line simultaneously and under any illumination condition, obtain fully the temperature value that recovers, calculate the light when obtaining any restoring degree simultaneously, the temperature value.
Above-mentioned sterile line material is from the offspring of 38 different cytoplasm donors hybrid combination, and having 630 parts of sterile strains is that the highest male sterile line reached for 13 generations from generation to generation.
Above-mentioned sterile line material the highest male sterile line from generation to generation is maternal with upland rice " long fragrant glutinous " work, and paddy rice " the bamboo cloud is glutinous " does 5 sisters systems of 86-21 systems of paternal hybrid.
Good male sterile line is the key of heterosis utilization.In sum, the present invention provides the method for the stable excellent sterile system of seed selection rice class quite perfectly, people can select various light temperature ecoclines big water, upland rice variety to carry out artificial hybridization as the parent according to actual needs, successfully obtain various good sterile line material according to method of the present invention.Seed selection step of the present invention is easy, and disposable hybridization or three is handed over and can be obtained to reverse the dual-purpose male caryon sterile line, and selection repeatability reaches 10%.The present invention regenerate the direct method saving of labor, save time, sterility is stable fast, because in the strict discriminating of the male sterile phenomenon that first familiar generation is occurred, be confirmed to be the combination that the fertility conversion can take place along with the change of weather conditions, just begin next step seed selection work, reduced the many blindness in the seed selection work.The problem of aspects such as the cytoplasm donor that the invention solves seed selection rice class male sterile line prior art is single, hybrid vigour makes up and breeding for disease resistance is subjected to very big limitation and sterile line material genetic background is not clear, method poor stability.
The reversible dual-purpose male nucleus sterile line of rice material that selects according to said method has following advantage:
(1) sterility is stable, sterile degree height since the pollen abortion time take place more early, occur in meiosis telophase I and latter stage II, the pollen abortion degree reaches 10%, sterile degree is all near 10%.Nucleosis type in the male sterile line pollen abortion type of the present invention and the various male sterile pollen abortion types of reporting before this have essential distinction, abortion thoroughly, take place early, more stable consistent sterile.Thereby seed production purity and stability have been guaranteed.
(2) the comprehensive proterties of male sterile line is good, and sterility is stable, the economical character neat and consistent, and economic characters are good, and coordinate force is good, and production application is had good practical value.
(3) recover the self-fruitful rate height, generally between 50-91%, be up to 91%, be convenient to the preparing hybrid kind, help improving the output of crossbreed.
(4) donorcells matter type is abundant, can avoid because the advantage that single cell matter is brought is bad, and disease heavily waits disadvantage.
(5) male sterile line is applied widely, and it is powerful generally to show advantage after indica cms line and the upland rice hybridization, and restoring degree is good, is the good male sterile line of carrying out water, the inferior distant hybridization of upland rice Xian round-grained rice.The japonica rice sterile line material cooperates advantage the strongest with long-grained nonglutinous rice or upland rice, also can hybridize with japonica rice, and advantage is not as the former, and this material also is a good material of carrying out water, non-irrigated Hybrids of Indica and Japonica.The disease-resistant drought resistance of Xian round-grained rice osculant male sterile line is all strong, is the male sterile line of special water distribution, the inferior distant hybridization of non-irrigated Xian round-grained rice.For strong advantage combination provides good material.
(6) the recovery source is wide, and test cross kind 75% all can be recovered, more screening leeway is arranged, having crossability widely, both can hybridize with long-grained nonglutinous rice, can hybridize with japonica rice again, increased the probability of screening strong advantage combination greatly.Thereby make the first-filial generation advantage powerful, for good basis is laid in heterotic utilization.
(7) the fertility inversion temperature is stable, and the sterile phase expanded to more than February, can reach 2-3 months in area, Xishuangbanna, was very beneficial for the production of hybrid seeds.
(8) proposed light temperature complementarity principle and test cross method and computing formula that the reversible dual-purpose male nucleus sterile line of rice fertility is changed, the qualitative, quantitative research of this temperature about the fertility conversion, optical condition has been laid excellent basis for practical application from now on.
Technical scheme of the present invention is as follows:
1.IVA system's sterile line breeding program:
2.MIVA system's sterile line breeding program:
3.87-9 system's sterile line breeding programs
4. the rice class can reverse the approach that utilizes of male sterile line:
The breeding of high latitude long-day in summer high temperature
Low latitudes spring, the autumn short-day low temperature production of hybrid seeds
Table one: the segregation ratio of several sterile combination F1
| The combination title | Total strain number | Sterile strain | Can educate strain | Can educate strain percentage | Sterile ratio can be educated |
| [462 * (459 * bamboo cloud is glutinous)] * 86-21-10-3 | ???409 | ????310 | ????99 | ?24.2 | ????3∶1 |
| (459 * bamboo cloud is glutinous) * 86-14-4-2 | ???550 | ????407 | ????143 | ?26.0 | ????3∶1 |
| (459 * bamboo cloud is glutinous) * 86-79-25-2 | ???534 | ????400 | ????134 | ?25.1 | ????3∶1 |
| IVA (short blood is glutinous * V20) | ???163 | ????43 | ????120 | ?73.6 | ????1∶3 |
According to above-mentioned technical scheme, seed selection large quantities of better performances rice class male sterile line and recover system, and several strong advantages combinations have been filtered out, set up and utilized the rice class can reverse the pattern of three approach of male sterile line, and proved that these three approach of employing are used sterile lines seed making of the present invention on producing and breeding is practicable.
Description of drawings:
Fig. 1 is seed selection overall plan figure for rice class two;
Fig. 2 is a seed selection general technical route map for rice class two;
Fig. 3 is the selection figure of ABA type male sterile line;
Fig. 4 is 86-21 system's sterile line breeding programme diagrams.
More in depth set forth flesh and blood of the present invention below in conjunction with accompanying drawing with embodiment:
Embodiment one: in order more in depth to understand technical scheme of the present invention, below set forth principle of the present invention and method with a complete concrete test procedure and result.
In order to make the male sterile line of breeding have higher self-fruitful rate and to have crossability widely, select the big parent of light temperature ecocline for use, with original bare hull round-grained rice type upland rice and the strong short bar paddy rice cross breeding of response to temperature in Xishuangbanna, F
1The ratio of pollen and the pollen that do not dye of can dyeing when doing rice culture early was near 1: 1, self-fruitful rate is very low, ratoon rice or summer are during rice culture, dyeing pollen reaches more than 90%, self-fruitful rate reaches combination more than 60% as the object of selecting ABA type male sterile line, generally, just can breed stable dual purpose genic male sterile line by steps such as hybridizing, differentiate, screen, continuous individual plant is selected and stablize.The method of its seed selection dual purpose genic male sterile line as mentioned above.The key technology measure is parental apolegamy and F
1The discriminating in generation.
Hybridization F
1The male sterile phenomenon that generation occurs can be by due to the multiple reason, and this just need hang down from generation to generation differentiates, can select to have genetic reversible dual purpose genic male sterile line.Also can reduce F effectively
2The blindness in generation.Unique direct-vision method of differentiating is with these F
1In generation, be combined in season plantation early, lets alone regeneration, allows same combination be under the different light and temperature conditions and grow, primary part observation fertile flower powder ratio and self-fertility situation, fertile flower powder ratio is lower than 50% combination, and probably offspring's male sterile line recovers the self-fertility difficulty, and is generally inadvisable.By differentiating, be confirmed to be the combination that the fertility conversion can take place along with the change of weather conditions, just begin next step seed selection work.
The hybridization F of seed selection ABA type male sterile line
2Generation, need cultivate in early spring, let alone under the short-day cryogenic conditions, to enter ear differentiation, finish reduction division.
Pass through F
1Combination after generation is differentiated under suitable light and temperature condition, generally can both be isolated sterile strain, and it is sterile mainly to be 3: 1 or 1: 3 (seeing Table one) with ratio that can educate.
From F
2In the sterile strain that generation separates, select plant height short partially (below 70 centimetres), comprehensive proterties individual plant good, that the pollen abortion degree is high to let alone to regenerate solid.The sterile strain of ABA type ties up to area, Xishuangbanna and can breed twice in 1 year, early season ratoon rice solid after, the all right autumn is once numerous, sowing at the beginning of 7 months, September on, ear the middle ten days, self-fertility still, carry out individual plant in convalescence selects in the same old way, so repeat to select 7 to 8 generations, 4 years, just can breed the male sterile line of sterility and comprehensive proterties quite stable.
Embodiment two: according to principle described in the embodiment one, introduce the selection and the characteristic thereof of wherein six preferred male sterile lines that the present invention selects below:
1,86-21-0-8-2A (elder brother plants S-1), the seed selection process as mentioned above.The parent is " long fragrant glutinous (upland rice) " * " bamboo cloud glutinous (paddy rice) ", and reached for 13 generations this year, belongs to Xian round-grained rice osculant, ABA type male sterile line, and under colony's situation of strain more than 2300, sterile plant rate and pollen sterile all reach 100%.Planting the sterile phase spring in Xishuangbanna reaches 70 days, and planting the sterile phase autumn also has 60 days, fertility inversion temperature height, illumination in 13 hours, day samming is sterile below 25.5 ℃, the pollen abortion approach belong to the nucleosis type (the nucleosis type is meant when male sterile line and is in sterile critical light and temperature condition when following, pollen mother cells latter stage I and latter stage II, can not form cell plate, thereby can not form normal two, tetrad, and form 4,6,8,16 or more apocyte.The nuclear disintegration takes place after forming pollen wall in these apocytes, and inclusion is oozed out, pollen adhesion, abortion).Convalescence, self-fruitful rate was up to 50%, and outcrossing seed-setting rate is up to 80%.The comprehensive proterties of this male sterile line is good, and disease resistance is strong, high resistance to hoja blanca especially, and thousand kernel weight 43 grams cooperate advantage the strongest with long-grained nonglutinous rice.
2,86-21-0-8-20A, the seed selection process as mentioned above.Be the sisters system of 2A, thousand kernel weight 45 grams, the long-pending intermediate value of fertility convert light temperature is lower, is 5558, about sterile 30 days phases, recovers self-fruitful rate 60%, and the sterile line propagation field is produced 240 kilograms.Belong to ABA type male sterile line, the pollen abortion approach is the nucleosis type.
3, IVA-1-2-1, the seed selection process as mentioned above.Indica type, paddy rice glutinous rice, sterile plant rate 100%, sterile degree is more than 99.95%, and it is very strong to tiller, and every mu of seedling number of seed farm can reach more than 300,000, sword-like leave is lacked and tiltedly exhibition, about 70 of every fringes, thousand kernel weight 25 grams, Hua Shizao, open the grain husk time more than 80 minutes, different friendship performance is good, and anti-white rot is the strongest with advantage behind the upland rice strain cross.The convert light temperature is amassed intermediate value 6954, about sterile 30 days phases, recovers self-fruitful rate and is up to about 80%.
4, IVA-1-2-6-3, the seed selection process as mentioned above.Parental source is " short blood is glutinous " * " V20 ", and indica cms line is named and is " elder brother plants S-2 ", and sterile plant rate and sterile degree all reach 100%, and convert light temperature value is illumination in 13 hours, 23.93 ℃ day samming following be sterile.The sterile phase is about 30 days when Xishuangbanna work rice culture morning, and self-fruitful rate is 68%, and outcrossing seed-setting rate reaches as high as 100%.Comprehensive proterties is good, about 45 centimetres of plant heights, and it is very strong to tiller, thousand kernel weight 26 grams, this male sterile line cooperates the performance advantage the strongest with upland rice strain system.
5, MIVA-2-10-3-3-4, the seed selection process as mentioned above.Japonica rice sterile line, under the situation of 2400 group of hill bodies, sterile plant rate and sterile degree all reach 100%, convert light temperature value is illumination in 13 hours, 21.48 ℃ day samming following be sterile.40 centimetres of plant heights, it is very strong to tiller, and makes rice culture early in Xishuangbanna, about sterile 35 days phases.Convalescence self-fruitful rate 66.8%.Glutinous rice, thousand kernel weight 22 grams, plant type is good, and sword-like leave is short, and fringe is upright, is convenient to cross pollination, cooperates advantage the strongest with long-grained nonglutinous rice.
6,87-9-6-2-1-1, the seed selection process as mentioned above.Be to make female parent with long-grained nonglutinous rice, upland rice forms as paternal hybrid, keeps the fringe grain type of Gui 630 substantially, and is indica type, glutinous rice, and breeding time is long, is late-maturing male sterile line, and fertility convert light temperature value is high, production of hybrid seeds insurance.
In addition, also have some comprehensive proterties good, the novel male sterile line that differs from one another does not exemplify one by one at this, but comes the seed selection male sterile line so long as belong to the thinking of selection of the present invention, all should think the scope that belongs to claim of the present invention.
Embodiment three: the present invention has also done the rice class can reverse the pollen abortion approach of dual purpose genic male sterile line and the test of sterile stability relationship.According to inventor's observation, the pollen abortion approach of dual purpose genic male sterile line has two kinds.A kind of is to be the pollen abortion approach of characteristic feature with the nucleosis; Another kind is the pollen abortion approach that deteriorates to characteristic feature with microspore monokaryon in late period.Because nucleosis type abortion period, early during reduction division, thereby the stability of sterility obviously was better than examining involution form.
According to inventor's cross experiment, make female parent with upland rice or the strong paddy rice of photosensitivity, make paternal hybrid with the response to temperature paddy rice, generally produce nucleosis abortion approach; And make female parent with paddy rice or upland rice, make paternal hybrid with the short bar upland rice of the modified form with upland rice blood relationship strain system, the general nuclear degeneration abortion approach that produces.It is maternal that " but land-reclaimable 58 " are done, and paddy rice makes paternal hybrid, also produces nuclear degeneration abortion approach (seeing Table two).And three friendships or transformation male sterile line do not change original abortion approach.
Table two, nucleosis and two kinds of abortion approach of nuclear degeneration and parent's relation
| The abortion type | Numbering | The combination title |
| Nuclear involution form | ?89-493 | 88-73-6 * 659 milli figure |
| ?90-228 | (wide extensive No. two * 85-1-15-1-1) * red 410 | |
| ?90-250 | (milli figure is * V20) * short blood is glutinous | |
| ?90-254 | (land-reclaimable 58 * Guizhou 416) * Guizhou 3+4 | |
| ?89-248 | 85-1-15-1-1 * (Gui 630 * 452)-A-17-1 | |
| ?87-9 | Gui 630 * 701 millis are old | |
| ?87-466 | 401×85—1—24—3 | |
| ?88-61 | 85-1-15-3-1 * wide extensive No. two | |
| ?87-347 | 85-1-24-3 * milli figure | |
| The nucleosis type | ?90-183 | (88-73-6 * V20) * red 410 |
| ?89-576 | 87-91-10-1-1 * yellow shell is glutinous | |
| ?89-58 | 86—14—4—7×88—493 | |
| ?89-155 | (86-100-49-9 * 86-14-4-4) * Wen Xuanqing | |
| ?88-142 | 462 * (long fragrant glutinous * the bamboo cloud is glutinous) * 86-74-2-2 | |
| ?IVA | Short blood is glutinous * V20 | |
| ?86-21 | Long fragrant glutinous * the bamboo cloud is glutinous |
Annotating: 88-73=85-1-15 * 87-1-1 85-1-15=schemes in the least * and the river is newly glutinous
87-91=(Xiao Bai paddy * close positive 23) * Gui 630 wide extensive No. two=Gui 630 * 632 nonirrigated farmland paddy
The general glutinous 86-14=910 of 86-100=ITAl18 * length * 476 fragrant glutinous choosings
87-1=Gui 630 * No. 14 * (Gui 630 of (459 * bamboo cloud is glutinous) 88-439=reaping hook paddy
* 452 is long fragrant glutinous)
Embodiment four: in the seed selection male sterile line, the present invention has paid attention to recovering the work of the strong advantage combination of seed selection, screening of system.Indica cms line as the test cross female parent is the male sterile line of IVA system, and japonica rice sterile line has MIVA system and each sisters system, and Xian round-grained rice osculant male sterile line has each sisters system of 86-21 systems.Recovering the owner as the test cross male parent will be from three massive material, first block of material, and a part is done institute from international rice, picks up from the medium rice new varieties of the Yunnan-Guizhou Plateau one band on a small quantity; Second largest block of material is drawn the japonica rice of short stem from the other places, comprises the japonica rice of Japanese type japonica rice and Taihu Lake basin; The third-largest block of material be after the present invention passes through to improve with indica hybrid of short stem with the original early rice in Xishuangbanna and rice varieties in, new lines of short stem.
By to F
1Observe and species test for advantage, from more than 1300 parts of test cross male parents, filter out eight wide spectrums and recover system.Principal character and parental source that these eight wide spectrums recover system see Table three.So-called broad spectrum activity recovers system, is exactly artificially to stipulate a kind simultaneously to indica cms line, and japonica rice sterile line and Xian round-grained rice osculant male sterile line all have good recovery capability, and have certain heterosis, hybrid vigor, and such kind is recovered system broad spectrum activity.This testcross and screening work has been carried out 3 years, hybridization F
1Hybridization F is adopted in the cultivation in generation and observation
1In generation, made rice cultivation, the late June sowing.Divide the combination cultivation, compare with ' Shanyou 63 '.Density is 6 * 10 cun, and single this is inserted.Control material plantation triplex row, totally 21 strains, hybridization F
1For all plantations of each combination.Main projects such as field observation tillering ability, disease resistance, strain are leaf, breeding time.The species test of drawing materials after the maturation, the contrast Shanyou 63 is got 5 strain species tests, hybridization F
1Whole strain numbers of planting in generation are all brought species test, and when meter produced, combination had only 50% product by actual production of 1 strain, and the combination of 2 strains is by 75% product, and 3 strains are by 80% product, and 4 strains are by 90% product, and 5 strains are above by 100% product.
Eight broad spectrum activities recover to show in big (the seeing Table four) table four of heterosis, hybrid vigor yield potentiality in system and three kinds of dissimilar reversed male sterile lines test cross F1 generations the hybridization of female parent of 8 male parents and three systems, all can make these three kinds dissimilar male sterile lines recover fertility, the yield potentiality of part combination is bigger.
Eight broad spectrum activities recover system the recovery capability of three kinds of male sterile lines are seen Table five.Find out that from table five it is 76% that average restoring degree removes extensively extensive No. two ripening rates, can both reach more than 80%.
Male parent and three kinds of dissimilar reversed male sterile lines test cross F
1The main biological property in generation sees Table six.
Male parent and three kinds of dissimilar reversed male sterile lines test cross F
1The main economic characters in generation see Table seven.Male parent and three kinds of dissimilar male sterile line test cross F in table seven
1Nearly all have more than 140 for number of grain per ear, the highest reaches 272.8, and more than 28 grams, the highest 52.4 grams that reach can obtain higher single plant yield to thousand kernel weight substantially.The big grain of big fringe is to constitute F
1Principal element for yield heterosis.
The present invention with eight broad spectrum activities recover system with three and type male sterile line test cross more than 70 combinations, 83.3% combination F is arranged
1Surpass the contrast Shanyou 63 for output.Wherein wide extensive F of hybridizing with IVA for No. two
1In generation, annual individual plant biological yield all surpasses Shanyou 63.In variety comparative test in 1991, output surpasses Shanyou 63 11%.
The screening broad spectrum activity recovers cording very big using value, as long as various places have above-mentioned three types male sterile line and this eight broad spectrum activities recover system, just can be according to the needs different hybrid combination of preparation arbitrarily of peasant household, to meet the needs of production.
Do the work of the strong advantage of maternal testcross and screening combination with reversible dual-purpose male nucleus sterile line of rice of the present invention, by to 1500 parts of test cross F
1Show for observed result: about 11% combination output surpasses the contrast Shanyou 63.The combination that repeated test cross performance high yield in 2 years has excellent No. two of drought, IVA-1-2-1 * imperial Mortopl, 86-21 * parent 37, non-irrigated excellent No. six etc.From the compatibility of male sterile line, it is powerful that the indica cms line of IVA system and round-grained rice type upland rice paternal hybrid generally show advantage; The Xian round-grained rice osculant male sterile line of 86-21 systems and indica hybrid performance advantage are powerful; MIVA system japonica rice sterile line and indica hybrid advantage are powerful.Be the characteristic of several strong advantage combinations below:
(1) drought is excellent No. two: hybrid combination is IVA-1-2-1 * wide extensive No. two, is Xian, round-grained rice water, the inferior distant hybridization combination of upland rice.Glutinous rice.Yielding ability is good, and 521 kilograms of paddy field variety comparative test average yield per mus in 1991, comparison be according to 51.5 kilograms of the level volume increase of ' Shanyou 63 ' 464.5 kilograms, and rate of growth 11% is 88-447 every mu (2 38.5 kilograms of 282.5 kilograms of volume increase, rate of growth 84.4% than upland rice strain.In the 120 days time of infertility, mu area day production capacity is 4300 grams.Plant height 125cm, every mu of effective fringe 10.4 ten thousand, 159 of average every fringes, ripening rate 80.3%, thousand kernel weight 38 grams.Disease resistance is medium, and this combination is main by the big grain of big fringe high yield.The nonirrigated farmland, paddy field all can be planted, and drought resistance and original upland rice are approaching.
(2) drought is excellent No. 6: hybrid combination is 86-21-0-8-20A * wide extensive No. 6, is drought, water
The main biological property characteristic and the source of table three, male parent
| Numbering | Kind, family name | Parent's title | Type | The time of infertility (my god) | Plant height (cm) | Thousand kernel weight (gram) | Every fringe strain spike number | Number of grain per ear (grain) | The real grain of every fringe number (grain) | Ghost rate (%) | Individual plant paddy heavy (gram) |
| Wide extensive No. 1 | 86-107 | The glutinous No. three * milli in a small bay in a river river is schemed bright | Round-grained rice, glutinous | 171 | ?85 | ?27 | ?13 | ?122.2 | ?110.4 | ?9.66 | ?28.3 |
| Wide extensive No. 2 | Gui 630 * 632 | Gui 630 * 632 | Xian, sticking, non-irrigated | 162 | ?97 | ?35.5 | ?8 | ?185.8 | ?157.9 | ?15.0 | ?37.5 |
| Wide extensive No. 3 | 87-213 | Glutinous * Yun Nong is glutinous for big grain | Xian, glutinous | 153 | ?95 | ?35 | ?10 | ?205.5 | ?182.6 | ?11.1 | ?46.7 |
| Wide extensive No. 4 | 86-79 | Wide precious 17 * Da Bai is glutinous for dragon | Xian, sticking, non-irrigated | 164 | ?90 | ?43.5 | ?11.7 | ?67.4 | ?54.9 | ?18.6 | ?23.0 |
| Wide extensive No. 5 | 87-86 | 81-2 * dragon is wide by precious 17 | Xian, glutinous | 170 | ?95 | ?33 | ?15 | ?158.5 | ?123.9 | ?21.8 | ?43.5 |
| Wide extensive No. 6 | No. 3, Hunan | The academy of agricultural sciences, Hunan introduces | Xian, sticking | 142 | ?70 | ?24 | ?17 | ?165.1 | ?162.6 | ?1.5 | ?44.2 |
| Wide extensive No. 8 | The dragon Mortopl | Introduce from Fujian | Xian, sticking | 158 | ?92 | ?25 | ?16.7 | ?187.3 | ?175.7 | ?6.2 | ?55.3 |
| Wide extensive No. 10 | N90-288 | Gather from Guizhou | Xian, sticking | 152 | ?100 | ?21 | ?12 | ?228.2 | ?179.8 | ?21.2 | ?40.2 |
Table four, eight broad spectrum activities recover the male sterile line test cross F of system and three systems
1For ripening rate and yield heterosis
| Maternal project male parent | 86-21 system osculant | The IVA system is indica type | MIVA system round-grained rice type | On average | ||||
| Output kg/ mu | Ripening rate | Output kg/ mu | Ripening rate | Output kg/ mu | Ripening rate % | Output kg/ mu | Ripening rate % | |
| Wide extensive No. 1 | 409.80 | ?87.23 | ?944.40 | ?84.31 | ?420.96 | ?88.63 | ?591.71 | ?86.72 |
| Wide extensive No. 2 | 750.60 | ?77.46 | ?1260.0 | ?72.90 | ?561.00 | ?78.73 | ?850.20 | ?76.36 |
| Wide extensive No. 3 | 974.25 | ?88.01 | ?544.50 | ?89.60 | ?486.90 | ?88.96 | ?668.55 | ?88.86 |
| Wide extensive No. 4 | 492.36 | ?87.94 | ?646.56 | ?73.01 | ?156.00 | ?90.88 | ?429.64 | ?83.94 |
| Wide extensive No. 5 | 489.00 | ?76.84 | ?260.87 | ?87.77 | ?481.50 | ?88.08 | ?410.46 | ?84.23 |
| Wide extensive No. 6 | 746.76 | ?72.33 | ?974.72 | ?95.71 | ?543.80 | ?73.50 | ?755.09 | ?80.51 |
| Wide extensive No. 8 | 680.00 | ?72.30 | ?749.70 | ?88.18 | ?496.80 | ?89.02 | ?548.00 | ?83.17 |
| Wide extensive No. 10 | 567.00 | ?89.47 | ?624.00 | ?88.33 | ?453.00 | ?86.17 | ?633.17 | ?87.34 |
| On average | 638.72 | ?81.45 | ?750.60 | ?84.73 | ?440.87 | ?85.50 | ||
Table five, eight universals recover the recovery of system to three types of male sterile lines
| It is title that maternal ghost rate % recovers | The 88-21 system | The IVA system | ????MIVA1 | On average | Ripening rate % |
| Wide extensive No. 1 | ????12.8 | ????15.7 | ????11.4 | ????13.3 | ????87.6 |
| Wide extensive No. 2 | ????22.5 | ????27.1 | ????21.3 | ????23.6 | ????76.4 |
| Wide extensive No. 3 | ????12.0 | ????10.4 | ????12.0 | ????11.4 | ????88.6 |
| Wide extensive No. 4 | ????12.1 | ????27.0 | ????9.1 | ????16.1 | ????83.9 |
| Wide extensive No. 5 | ????23.2 | ????12.2 | ????11.9 | ????15.8 | ????84.2 |
| Wide extensive No. 8 | ????27.7 | ????5.3 | ????26.5 | ????19.3 | ????80.2 |
| Wide extensive No. 8 | ????27.7 | ????11.8 | ????11.0 | ????16.8 | ????83.2 |
| Wide extensive No. 10 | ????10.5 | ????13.6 | ????13.8 | ????12.6 | ????87.4 |
| On average | ????18.56 | ????15.14 | ????14.62 |
Table six, this test cross of father and mother F1 learn characteristic for important biomolecule
| Seeing a maternal survey recovery order is title | The 86-21 system | The IVA system | ??????????MIVA1 | |||
| Plant height (cm) | The time of infertility (my god) | Plant height (cm) | The time of infertility (my god) | Plant height (cm) | The time of infertility (my god) | |
| Wide extensive No. 1 | 100 | ?110 | ?120 | ?114 | ?95 | ?100 |
| Wide extensive No. 2 | 143 | ?116 | ?105 | ?119 | ?90 | ?112 |
| Wide extensive No. 3 | 115 | ?103 | ?105 | ?116 | ?90 | ?103 |
| Wide extensive No. 4 | 100 | ?103 | ?100 | ?107 | ?90 | ?115 |
| Wide extensive No. 5 | 115 | ?103 | ?115 | ?132 | ?95 | ?118 |
| Wide extensive No. 6 | 105 | ?103 | ?80 | ?107 | ?105 | ?100 |
| Wide extensive No. 8 | 110 | ?94 | ?105 | ?110 | ?93 | ?92 |
| Wide extensive No. 10 | 112 | ?95 | ?120 | ?113 | ?103 | ?102 |
Table seven, the main economic characters of this test cross of father and mother F1
Rice hybridization combination, paddy field product be than 465.5 kilograms of output, than 4 kilograms of the Shanyou 63 underproduction, but precocious 13 days, nonirrigated farmland product are than 275 kilograms of per mu yields, and comparison is shone Shanyou 63 and is increased production 94%.The 106 days time of infertility, 12 leaves of stem, plant height 115cm, every mu of effective fringe 130,000,159 of number of grain per ears, ripening rate 87.7%, thousand kernel weight 36 grams.High bacterial leaf spot resistant, high blast resisting.This combination plant type more diffusing, the later stage discolor not as the drought excellent No. two good.
| It is title that maternal observation project is recovered | The 86-21 system | The IVA system | ????MIVA1 | ||||||||||||
| Single-strain tassel number | Number of grain per ear | Ghost rate (%) | Thousand kernel weight (gram) | Individual plant paddy heavy (gram) | Single-strain tassel number | Number of grain per ear | Ghost rate (%) | Thousand kernel weight (gram) | Individual plant paddy heavy (gram) | Single-strain tassel number | Number of grain per ear | Ghost rate (%) | Thousand kernel weight (gram) | Individual plant paddy heavy (gram) | |
| Wide extensive No. 1 | 8 | ?174.8 | ?12.7 | ?36 | ?34.2 | ?8 | ?200.2 | ?15.7 | ?32 | ?78.7 | ?11 | 164.7 | ?11.4 | ?28 | ?35.1 |
| Wide extensive No. 2 | 20 | ?272.8 | ?22.5 | ?38.3 | ?125 | ?9.8 | ?167.4 | ?27.1 | ?30 | ?105 | ?13 | 171.0 | ?21.3 | ?29.5 | ?46.6 |
| Wide extensive No. 3 | 16 | ?200.0 | ?12.0 | ?42 | ?108.3 | ?16.5 | ?134.6 | ?10.4 | ?35.3 | ?60.5 | ?16.5 | 183.5 | ?12.0 | ?28 | ?54.1 |
| Wide extensive No. 4 | 9.6 | ?142.6 | ?12.1 | ?52.6 | ?41.1 | ?17.4 | ?117.1 | ?26.9 | ?36.8 | ?53.9 | ?8 | 87.8 | ?9.1 | ?37 | ?25.5 |
| Wide extensive No. 5 | 15 | ?187.4 | ?23.2 | ?49.3 | ?81.5 | ?12.2 | ?147.2 | ?12.2 | ?37.3 | ?21.7 | ?16 | ?151.9 | ?11.9 | ?29 | ?35.5 |
| Wide extensive No. 6 | 14.8 | ?179.6 | ?27.7 | ?36 | ?62.2 | ?27 | ?160.7 | ?5.3 | ?28.8 | ?101.5 | ?12.8 | ?170.6 | ?26.5 | ?25 | ?45.3 |
| Wide extensive No. 8 | 11.6 | ?249.2 | ?27.7 | ?35 | ?56.7 | ?14.8 | ?181.9 | ?11.8 | ?31.7 | ?62.5 | ?11.7 | ?191.3 | ?11.0 | ?29 | ?43.5 |
| Wide extensive No. 10 | 8.4 | ?271.8 | ?10.5 | ?36 | ?47.3 | ?10.5 | ?227.7 | ?13.6 | ?31 | ?52.0 | ?10.3 | ?235.6 | ?13.8 | ?27 | ?37.8 |
(3) 86-21 * parent 37: the restoring degree height, yielding ability is good, and breeding time is short, makes rice cultivation, the 98 days time of infertility.Plant height 110cm, several 164 of the real grain of average every fringe, ghost rate 14%, the test cross performance is increased production than Shanyou 63 for successive years.This combination disease resistance is very strong, both bacterial leaf spot resistant, also blast resisting.Paddy field, nonirrigated farmland all can be planted, in the nonirrigated farmland live tiller very strong.This combination plant type is more diffusing.
Other have the strong advantage combination of the symptom of a trend to give an example no longer one by one.Embodiment five: with the reversed male sterile line that the present invention breeds, prepared ten combinations altogether, carrying out two is the discussion test of the production of hybrid seeds, and test material and result are as follows:
1, the key property that is used for the male sterile line of the production of hybrid seeds and recovers based material see Table eight, table nine, table ten.
The key property of the several male sterile lines of table eight
| The male sterile line title | Plant height (cm) | Duration from seeding to heading (my god) | The stem number of blade (sheet) | Individual plant tiller (individual) | Effective accumulated temperature (℃) |
| The IVA system | ????60 | ?105-110 | ????16-17 | ????10-15 | ????2216 |
| The 86-21 system | ????70 | ?85-90 | ????11-12 | ????5-8 | ????1855 |
The flowering habit of table nine male sterile line
| The male sterile line title | The Sheng of the blooming phase | Flower at morning (Z) | Afternoon flower (%) | The column cap activity (my god) |
| The IVA system | The beginning fringe after 7 days | About 40 | About 60 | ????3-5 |
| The 86-21 system | The beginning fringe after 11 days | About 30 | About 70 | ????3-4 |
Table ten recovers system source and key property
| Title | The source | Plant height (cm) | Duration from seeding to heading (my god) | Tillering ability (/ strain) | Number of grain per ear |
| Gestrinone | The academy of agricultural sciences, Hunan | ????95 | ?115-117 | ?12-13 | ?117.5 |
| No. three, Hunan | The academy of agricultural sciences, Hunan | ????60 | ?100-103 | ?11-12 | ?133.7 |
| The dragon Mortopl | Fujian | ????90 | ?120-124 | ?15 | ?241.5 |
| Close positive 43 | South Korea | ????65 | ?112-114 | ?9-10 | ?144.4 |
| IR35293 | Rice in China provides | ????80 | ?120-124 | ?13-14 | ?180.8 |
| Parent 37 | The academy of agricultural sciences, Hunan | ????70 | ?90-93 | ?13-14 | ?132.4 |
| Wide extensive No. two | This group | ????85 | ?120-125 | ?8-10 | ?169.3 |
2, producing method for seed, because of two being that the production of hybrid seeds also not have at present fixing pattern and method, so the production of hybrid seeds is to imitate three experiences that are, but two based materials have its exclusive characteristic, therefore, have done as the case may be to revise and additional.Mainly adopt in the sterile phase rationally and arrange sowing time (seeing Table 11), adopt big duplicate rows male parent divide the second phase sowing and use sufficient base manure, early spraying fertilizer, suitablely execute measure such as strong luxuriant fertilizer and come the production of hybrid seeds.Each makes up, and the hybrid seed yield structure sees Table 12, hybrid seed yield still is gratifying, and the highest per mu yield has reached 78 kilograms.
The sowing date and heading date of table ten each production of hybrid seeds combination
| The combination title | Sowing time (moon/day) | The transplanting phase (moon/day) | The fringe phase (moon/day) beginning | Full heading time (moon/day) | ||||||||
| One father | Two fathers | Maternal | One father | Two fathers | Maternal | One father | Two fathers | Maternal | One father | Two fathers | Maternal | |
| IVA-1-2-1 * imperial Mortopl | 12/29 | ?1/5 | ?1/3 | ?2/2 | ?2/2 | ?2/2 | ?5/2 | ?5/5 | ?4/22 | ?5/8 | ?5/12 | ?5/8 |
| No. 3, IVA-1-2-1 * Hunan | 12/29 | ?1/5 | ?1/1 | ?1/30 | ?1/30 | ?1/30 | ?4/13 | ?4/15 | ?4/18 | ?4/11 | ?4/20 | ?5/6 |
| IVA-1-2-1 * close positive 48 | ?12/29 | ?1/5 | ?1/3 | ??2/4 | ?2/4 | ?2/5 | ?4/23 | ?4/29 | ?4/24 | ?4/20 | ?4/31 | ?5/9 |
| IVA-1-2-1 * R-2323 | 12/29 | ?1/5 | ?1/8 | ??2/4 | ?2/4 | ?2/10 | ?4/26 | ?4/30 | ?4/25 | ?4/30 | ?5/3 | ?5/12 |
| IVA-1-2-1 ×IR3529 | ?12/29 | ?1/5 | ?1/18 | ??2/4 | ?2/4 | ?2/10 | ?5/2 | ?5/4 | ?5/2 | ?5/5 | ?5/7 | ?5/18 |
| IVA-1-2-1 * wide extensive No. 2 | 12/29 | ?1/5 | ?1/16 | ??2/4 | ?2/4 | ?2/10 | ?3/2 | ?5/7 | ?4/30 | ?5/9 | ?5/13 | ?5/15 |
| 20A * imperial Mortopl | 12/29 | ?1/5 | ?1/28 | ??2/2 | ?2/2 | ?2/28 | ?5/2 | ?5/5 | ?4/22 | ?5/8 | ?5/12 | ?5/7 |
| No. 3,20A * Hunan | 12/29 | ?1/5 | ?1/13 | ??1/30 | ?1/30 | ?2/9 | ?4/12 | ?4/15 | ?4/14 | ?4/16 | ?4/19 | ?4/29 |
| 20A * wide extensive No. 2 | 12/29 | ?1/5 | ?1/31 | ??2/4 | ?2/4 | ?3/2 | ?5/2 | ?5/7 | ?4/30 | ?5/9 | ?5/14 | ?5/16 |
| 3A * parent 37 | ?12/29 | ?1/5 | ?1/13 | ??2/4 | ?2/4 | ?2/8 | ?4/8 | ?4/12 | ?4/15 | ?4/14 | ?4/15 | ?4/29 |
Table ten two makes up output structure (the sub-district area is 60 square metres) fully
| Father and mother's written or printed documents project | ?????????????IVA—1—2—1 | ??20?????A | ?3A | ||||||
| R-2323 | No. 3, Hunan | Close positive 48 | Long Chipu | ????IR35293 | Wide extensive No. 2 | No. 3, Hunan | The dragon Mortopl | Parent 37 | |
| Maternal total spike number (ten thousand) | ?29.9 | ?37 | ?29.2 | ?31.5 | ????28 | ?27.5 | ?18.9 | ?19.2 | ?15.4 |
| Male parent is spent number (ten thousand) | ?485 | ?403 | ?448 | ?400 | ????775 | ?536.2 | ?403 | ?400 | ?662 |
| Cell production (kg) | ?6.6 | ?6.7 | ?4.6 | ????7.05 | ?4.1 | ?7.1 | ?2.05 | ||
| Amount to per mu yield (kg) | ?73 | ?74.5 | ?51 | ????78.5 | ?45.5 | ?22.5 | |||
Embodiment six: in order to inquire into the production capacity and the high-yield culture technique of two-line hybrid rice combination, promoting for large tracts of land provides scientific basis, the present invention also to be provided with the two groups of variety comparative tests in paddy field and nonirrigated farmland.Result of the test is:
1, yield result analysis, through variance analysis, its product interspecific difference has reached significance level (seeing Table 13) for each output that makes up of examination.Eight for having five per mu yields to surpass CK1 ' Shanyou 63 in the examination material '.There have four combination average per mu yields to be higher than the sharp fiber crops of CK ' milli to be precious ', its output precedence sees Table 14.Be several combinations preferably below:
(1) No. three, 20A * Hunan: yielding ability is good, and this tests 550 jin of average yield per mus, and than 267 jin of Shanyou 63 volume increase, rate of growth 94.3% is than 254 jin of the precious volume increase of the sharp fiber crops of milli, rate of growth 85.8%.Disease resistance is good, and rice blast harm is light, the 107 days time of infertility, and 12 of the stem numbers of blade, 110 centimetres of plant heights, it is medium to tiller, every mu of effective fringe 5.5 ten thousand, 22 centimetres of spike lengths, several 142 of the real grain of every fringe, the percentage of earbearing tiller 81.1%, thousand kernel weight 35 grams.
(2) IVA-1-2-1 * imperial Mortopl, yielding ability is good, 352 jin of nonirrigated farmland test average yield per mus, than Shanyou 63 volume increase 24.4%, than the precious volume increase 18.9% of the sharp fiber crops of milli, blast resisting, the 113 days time of infertility, 12 in the total blade of stem, 90 centimetres of plant heights, tillering ability is strong, every mu of effective fringe 6.4 ten thousand, several 94.8 of the real grain of every fringe, ripening rate 75.5%, thousand kernel weight 29 grams.
(3) No. three, IVA-1-2-1 * Hunan, and yielding ability is better, 315 jin of nonirrigated farmland test average yield per mus, than Shanyou 63 volume increase 11%, than the precious volume increase 6.4% of the sharp fiber crops of milli, blast resisting, the 114 days time of infertility, 12 of the stem numbers of blade, 90 centimetres of plant heights, 19.3 centimetres of spike lengths, tillering ability is strong, every mu of effective fringe 4.5 ten thousand, several 99 of the real grain of every fringe, ripening rate 80.7%, thousand kernel weight 28 grams.
(4) IVA-1-2-1 * wide extensive No. two, the yielding ability of this combination is similar to Shanyou 63, breeding time is also more approaching, tillering ability is stronger than Shanyou 63,146 of average every fringes, ripening rate about 81%, thousand kernel weight 39 grams, drought resistance and original upland rice are approximate, only breeding time longer, be suitable for the plantation of high temperature area.
2, the output structural analysis of each experimental cultivar, combination
(1) 10 IVA-1-2-1 * every mu of effective fringe of imperial Mortopl is maximum, is 6.4 sides in the material, the percentage of earbearing tiller 29.1%, and minimum is that the sharp fiber crops of milli are precious, every mu 3.5 ten thousand, the percentage of earbearing tiller 43.8%.
What (2) the real grain of average every fringe number was maximum is that 20A * Hunan is 142 for No. three, ripening rate 81.1%; Grain number per spike is minimum is No. three, IVA-1-2-1 * Hunan, is 76.8% ripening rate 62.6%.The kind that ripening rate is the highest is 88-206-13-1-1-2, several 114 of the real grain of every fringe, ripening rate 87.4%.
(3) the thousand kernel weight maximum is that the sharp fiber crops of milli are precious, thousand kernel weight 39 grams, and minimum is that Shanyou 63 is 27 grams.Each output structure for the examination material sees Table 15.
Table ten three-variable analysis table
| Mutagen because of | Bai Shandu | Sum of squares | Variable | ????F | The 5ZF value | ??F0.05 | ?F0.01 |
| Between kind | ?9 | ?84.695 | ?9.411 | ?9.411/10.134＝0.929 | ?10.904# | ????2.5 | ?3.6 |
| Between repetition | ?2 | ?0.509 | ?0.255 | ?0.255/10.134＝0.025 | |||
| Chance error | 18 | ?182.41 | ?10.134 | ||||
| Total variation | 29 | ?97.202 |
Annotate: # represents that difference reaches one-level significance level
Table ten four variety comparative test cell production results
| Project output (jin) combination title and contrast | The replicated plot | Add up to (jin) | Amount to per mu yield (jin) | Comparison is according to volume increase (%) | Precedence | Remarks | |||
| ?I | ?II | ?III | |||||||
| Contrast I | Contrast II | ||||||||
| Shanyou 63 CK1 | ?4.9 | ?5.6 | ?4.8 | ?15.3 | ?283 | ?— | ?— | ?7 | |
| The sharp numb precious CK2 of milli | ?4.5 | ?6.3 | ?5.2 | ?16.0 | ?296 | ?— | ?— | ?5 | Upland rice |
| IVA—1—2—1× IR35293 | ?4.8 | ?5.0 | ?4.9 | ?14.7 | ?272 | ?— | ?— | ?8 | |
| IVA-1-2-1 * wide extensive No. 2 | ?5.3 | ?4.6 | ?5.6 | ?15.5 | ?287 | ?1.4 | ?— | ?6 | |
| IVA-1-2-1 * imperial Mortopl | ?6.1 | ?6.5 | ?6.4 | ?19.0 | ?352 | ?24.4 | ?18.9 | ?2 | |
| No. 3, IVA-1-2-1 * Hunan | ?6.2 | ?4.5 | ?6.3 | ?17.0 | ?315 | ?11.3 | ?6.4 | ?3 | |
| IVA-1-2-1 * close positive 48 | ?4.1 | ?3.0 | ?3.6 | ?10.7 | ?198 | ?— | ?— | ?9 | |
| IVA-1-2-1 * R-2323 | ?3.2 | ?3.5 | ?4.0 | ?10.7 | ?198 | ?— | ?— | ?9 | |
| No. 3,86-21-0-8-20A * Hunan | ?10.2 | ?9.0 | ?10.5 | ?29.7 | ?550 | ?94.3 | ?85.8 | ?1 | |
| 88—206—13 1—1—2 | ?4.3 | ?7.5 | ?5.1 | ?16.9 | ?313 | ?10.6 | ?5.7 | ?4 | |
15 various combination yield component tables
| The projects combo title | Basic seedling ten thousand/mu | Gao Miaowan/mu | The percentage of earbearing tiller (%) | Effective fringe ten thousand/mu | Average every fringe | Thousand kernel weight (gram) | ||
| The grain number | Real grain number | Ripening rate % | ||||||
| Shanyou 63 CK1 | ?1.1 | ????25 | ????21.2 | ????5.3 | ?124.2 | ????98 | ????78.9 | ????27 |
| The sharp numb precious CK2 of milli | ?1.1 | ????8 | ????43.8 | ????3.5 | ?169 | ????110 | ????65.1 | ????39 |
| ????IVA—1—2—1×IR35293 | ?1.1 | ????25 | ????15.6 | ????3.9 | ?155.7 | ????124.9 | ????80.2 | ????28 |
| IVA-1-2-1 * wide extensive No. 2 | ?1.1 | ????24 | ????19.6 | ????4.7 | ?148.7 | ????95.7 | ????64.4 | ????32 |
| No. 3, IVA-1-2-1 * Hunan | ?1.1 | ????27 | ????21.1 | ????5.7 | ?123 | ????99.2 | ????80.7 | ????28 |
| IVA-1-2---1 * close positive 48 | ?1.1 | ????25 | ????16.8 | ????4.2 | ?126.6 | ????81.5 | ????64.4 | ????29 |
| IVA-1-2-1 * R-2323 | ?1.1 | ????24 | ????18.8 | ????4.5 | ?122.6 | ????76.8 | ????62.6 | ????29 |
| IVA-1-2-1 * imperial Mortopl | ?1.1 | ????22 | ????29.1 | ????6.4 | ?125.3 | ????94.8 | ????75.7 | ????29 |
| No. 3,20A * Hunan | ?1.1 | ????22 | ????25 | ????5.5 | ?175 | ????142 | ????81.1 | ????35 |
| ????88—206—13—1—1—2 | ?1.1 | ????18 | ????22.2 | ????4.0 | ?130.6 | ????114.1 | ????87.4 | ????35 |
Claims (9)
1. the selection of reversible dual-purpose male nucleus sterile line of rice, it is characterized in that breeding sterile line material by steps such as hybridizing, differentiate, screen, continuous individual plant is selected, stablized, said hybridization is to select for use big paddy rice of light temperature ecocline or upland rice variety to carry out artificial hybridization as the parent, and said discriminating is with the F after the hybridization
1In generation, made rice culture early, chooses the fertile flower powder and the pollen sterile ratio is regenerated near 1: 1 combination, and said screening is to choose ratoon rice or summer the fertile flower powder reaches more than 90% during rice culture, self-fruitful rate is near the F of conventional rice variety
2In generation,, said continuous individual plant is selected and be stable was from above-mentioned F in the cultivation in early spring
2In the sterile strain that generation separates, select short partially, the comprehensive proterties of plant height individual plant good, that the pollen abortion degree is high to let alone to regenerate solid, so repeat to select 7 to 8 generations or advanced lines more, then breed stable reversible dual-purpose male nucleus sterile line of rice.
2. selection according to claim 1 is characterized in that the parent selects one to be strong water, the upland rice variety of photosensitivity in the crossover process, and another is the strong water of response to temperature, upland rice variety.
3. selection according to claim 1 is characterized in that it is spring of 20-25 ℃ that described F2 is planted in daily mean temperature for colony.
4. selection according to claim 1 and 2 is characterized in that described cross method adopts direct method, three friendship methods or backcross method to let alone a kind of.
5. the reversible dual-purpose male nucleus sterile line of rice material that selection according to claim 1 obtains, it is characterized in that described sterile line material is the ABA type, the fertility switching mechanism is a light temperature complementation, its fertility changes with the change of light and temperature condition, under the end of spring and the beginning of summer low temperature short-day condition is sterile, recover self-fertility under high temperature long-day in the midsummer condition, transfer to sterile autumn, the light temperature value that fertility changes is 13h/24-26 ℃, the sterile phase is 1 to 3 months, and sterile degree reaches more than 99.9%.
6. sterile line material according to claim 5 is characterized in that the recovery source is wide, and 75% test cross kind all can have the solid ability of higher recovery to male sterile line, and the highest recovery ripening rate can reach 90%.
7. sterile line material according to claim 5 is characterized in that described the highest male sterile line of generation is to make female parent with upland rice " long fragrant glutinous ", and paddy rice " the bamboo cloud is glutinous " does 5 sisters systems of 86-21 systems of paternal hybrid
8. according to claim 5 or 6 or 7 described sterile line material, it is characterized in that being divided into the indica cms line high, cooperate the strong japonica rice sterile of advantage with long-grained nonglutinous rice or upland rice and cooperate the Xian round-grained rice osculant male sterile line that advantage is strong, disease resistance is strong, drought resistance is strong with long-grained nonglutinous rice with the upland rice hybridization back strong advantage of performance and restoring degree.
9. sterile line material according to claim 5 is characterized in that the pollen abortion approach is that pollen abortion occurs in meiosis telophase I, pollen abortion is thorough, and the abortion degree reaches 99-100% nucleosis type and nuclear involution form.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103975846A (en) * | 2014-05-15 | 2014-08-13 | 江西省农业科学院水稻研究所 | Novel method for creating dual-purpose rice genic male sterile line |
| CN104774845A (en) * | 2014-01-15 | 2015-07-15 | 中央研究院 | Mutational nucleotide molecule, transformed plant cell comprising same, and method for preparing recoverable male sterile transgenic plant |
| CN106069453A (en) * | 2016-07-19 | 2016-11-09 | 莆田秀屿区群韬农业技术开发有限公司 | The breeding method of a kind of drought resisting rice and implantation methods |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1014483B (en) * | 1989-08-04 | 1991-10-30 | 张贵芝 | Method for producing hybrid wheat |
| CN1026282C (en) * | 1990-03-29 | 1994-10-26 | 福建农学院 | Ways to improve hybrid rice through hybridization by two series methods |
-
1993
- 1993-10-21 CN CN93118879A patent/CN1041988C/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104774845A (en) * | 2014-01-15 | 2015-07-15 | 中央研究院 | Mutational nucleotide molecule, transformed plant cell comprising same, and method for preparing recoverable male sterile transgenic plant |
| CN104774845B (en) * | 2014-01-15 | 2018-02-27 | 中央研究院 | Mutant nucleotide acid molecule and conversion plant cell comprising it and prepare the method that can recover male sterility genetically modified plants |
| CN103975846A (en) * | 2014-05-15 | 2014-08-13 | 江西省农业科学院水稻研究所 | Novel method for creating dual-purpose rice genic male sterile line |
| CN106069453A (en) * | 2016-07-19 | 2016-11-09 | 莆田秀屿区群韬农业技术开发有限公司 | The breeding method of a kind of drought resisting rice and implantation methods |
| CN106069453B (en) * | 2016-07-19 | 2019-06-04 | 新昌县大船畈生物科技有限公司 | A kind of breeding method and implantation methods of drought resisting rice |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1041988C (en) | 1999-02-10 |
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