CN1903013A - Method for breeding improved hybrid rice with changed content of amylose - Google Patents
Method for breeding improved hybrid rice with changed content of amylose Download PDFInfo
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- CN1903013A CN1903013A CNA2005100122692A CN200510012269A CN1903013A CN 1903013 A CN1903013 A CN 1903013A CN A2005100122692 A CNA2005100122692 A CN A2005100122692A CN 200510012269 A CN200510012269 A CN 200510012269A CN 1903013 A CN1903013 A CN 1903013A
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Abstract
A breeding method for culturing an improved hybrid rice containing moderate content of straight-chain starch features that a mixed hybrid rice with moderate content of straight-chain starch is cultured through hybridization between one female parent containing low content of straight-chain starch and chosen from the sterile paddy rice Wufeng A, Suifeng A, Yufeng A, and Yuguang S and two male parents which are one recovery line with low content of straight-chain starch and another recovery line with high content of straight-chain starch.
Description
Technical field
The present invention relates to a kind of breeding method that improves hybrid rice with changed content of amylose, belong to the rice heterosis utilization technical field.
Background technology
At present the main path of domestic and international indica type rice heterosis utilization comprise based on nucleo-cytoplasmic interaction such as wild abortion type or red lotus type three line method (male sterile line, maintenance line, recovery system) and based on the two line method of optical-and thermal-sensitive condition.Since China in 1973 at first realized the indica hybrid rice three series mating in the world, China kept world lead level in the rice heterosis utilization research field always.In the seventies in last century and the eighties, China produce to go up the Shan major clique prestige major clique system series of three-series hybrid rice of unifying and is rapidly developed; In the nineties in last century, China's Shan is excellent, prestige is excellent, the ridge is excellent, II is excellent, up to a hundred the Three-line Hybrid paddy rice that amount to of excellent, rich excellent and association's major clique system are combined in and have covered national most of long-grained nonglutinous rice district in the production.The long-term cultivated area of China's hybrid rice was about more than 1,500 ten thousand hectares in recent years, accounted for China's rice growing area more than 60%.At present, along with China rice consumers living's raising day by day, improve hybrid rice rice quality proterties and become the breeding objective that the rice breeding man more urgently pays close attention to.Studies show that amylose content is a most important physicochemical character of decision rice food flavor quality, and amylose content is 17% to 19% to be the best amylose content index of rice usually.Yet, because being the two poles of the earth, China's seed rice resource, the especially amylose content of long-grained nonglutinous rice germ plasm resource distribute, even be lower than 15%, or be higher than 25%, and the amylose content of the long-grained nonglutinous rice germplasm of most of modern improvement belongs to the high amylose content type.Research also shows, height * height → height is low * low → low, and low * height → higher parent.Therefore, owing to lacked the restriction of the germ plasm resource with medium amylose content, the hybrid rice that adopts traditional hybridization method for group matching to be difficult to breed to have best amylose content makes up.
Summary of the invention
The present invention is directed to the problem that causes the hybrid rice combination of adopting traditional hybridization method for group matching to be difficult to breed to have best amylose content owing to the restriction that is lacked germ plasm resource, propose a kind of breeding method that improves hybrid rice with changed content of amylose with medium amylose content.Purpose is recovered system's hybridization with high and low amylose content respectively by utilizing a low amylose content rice sterile line, breeds the commodity hybrid rice seed of adjustable amylose content.Technical scheme of the present invention is to utilize 1 rice sterile line five rich A, the rich A in Guangdong, the rich A of fringe or Guangdong light S with low amylose content to be female parent, has the recovery system of high amylose content and the recovery system hybridization that 1 has low amylose content with 1 respectively, hybrid seed mixes by a certain percentage, breeds the commodity hybrid rice combination with target amylose content; Be the male parent mass seed production with 2 recoveries perhaps, produce commodity-type bulk crossing kind with 2 single cross hybrid with different amylose contents with 1 low amylose content male sterile line.
According to above-mentioned know-why, in 2003 early season in Guangzhou with long-grained nonglutinous rice male sterile line germplasm " five rich A " with 13% left and right sides amylose content, have with 1 that low amylose content (being lower than 15%) recovers system " wide extensive 998 " and 1 recovery system " wide extensive 308 " with high amylose content (being higher than 26%) hybridizes respectively, produce " five Feng You 998 " and " five Feng You 308 " hybrid seed.Season in evening in 2003, the hybrid seed of " five Feng You 998 " and " five Feng You 308 " is mixed in the ratio of 1: 0.5,1: 0.75,1: 1 and 1: 1.25, be mixed with the plantation of bulk crossing seed.After the results, the commodity paddy of different proportion is fully mixed, carry out amylose content and detect, thereby acquisition has the hybrid seed formula rate of desirable amylose content.
The present invention compared with prior art has following advantage:
1. can cultivate hybrid rice combination with adjustable amylose content.
If adopt traditional breeding method, because of lacking germplasm in the existing hybrid rice parents resource with desirable amylose content, thereby the amylose content of the hybrid rice of breeding usually combination or on the low side, as with amylose content being the hybrid rice of having authorized the combination Feng You silk seedling (Guangdong Province's variety certification) that about 14% the rich A in long-grained nonglutinous rice male sterile line Guangdong breeds, Feng Youxiang accounts for (Jiangsu Province and national variety certification), Feng You 559 (Jiangsu Province and national variety certification) and make up five Feng You 998 (Guangdong Province's variety certification) with the hybrid rice that the long-grained nonglutinous rice male sterile line five rich A of amylose content about 13% breed; Or too high, as Feng You 128, five Feng You 128, five Feng You 308 etc.
And adopt our rule can overcome the defective that above-mentioned conventional method exists.As adopt five Feng You 998 and five Feng You, 308 single-cross seeds by 1: 0.75 formula rate, and the hybrid seed of production, the amylose content of its commodity paddy is about about 19%, thereby its meal flavor is all better than five Feng You 998 or five Feng You 308.
2. can improve the resistance of hybrid
Research shows that with application practice hybrid rice has more lasting and complementary resistance than conventional rice pure line cultivar usually because of its heterozygosity from the amphilepsis background.Therefore, the combination of female two fathers mixed type hybrid rice should have more desirable resistance than a female father single cross germplasm in theory.As adopt five Feng You 998 and 1: 0.75 formula rate of five Feng You, 308 hybrid seeds, the mixed type hybrid seed carries complementary rice blast resistance gene because of 2 male parents wide extensive 998 and wide extensive 308, promptly wide extensive 998 contain the resistant gene of " middle C " rice blast population, do not contain the resistant gene of " middle B " rice blast population; And wide extensive 308 contain middle B " resistant gene of rice blast population, do not contain the resistant gene of " middle C " rice blast population.Test shows that the bulk crossing kind is lower than 5% in Longchuan County, Guangdong Province institute of agricultural sciences rice blast lesion plantation ear stem pest incidence, and under the same conditions, the ear stem pest incidence of five Feng You 998 or five Feng You 308 all is higher than 20%.
3. can improve the annidation of hybrid
Research shows that with application practice hybrid rice has annidation widely than conventional rice pure line cultivar usually because of its heterozygosity from the amphilepsis background.Therefore, can expect that employing one female two fathers' mixed type crossbreed is planted, its annidation is wider than a female father single cross hybrid.Participate in south China Xian high-quality group district examination early in 2003 as five Feng You 998, in Guangdong, 8 district's pilot average yield per mus comparisons in Guangxi, Hainan and Fujian Province are according to kind of " Guangdong perfume (or spice) accounts for " volume increase less than 4%, do not reach significance level, and the bulk crossing kind participation south China Xian high-quality group district examination early with five Feng You 998 and five Feng You 308 in 2004, the average yield per mu comparison of bulk crossing kind reaches utmost point significance level according to kind of " Guangdong perfume (or spice) an accounts for " volume increase 13.8%.
Description of drawings:
Fig. 1 is the seed selection schematic diagram of modified form five Feng You 998.
Fig. 2 is the seed selection schematic diagram of modified form Feng You silk seedling.
Fig. 3 is the seed selection schematic diagram in modified form Guangdong two excellent 128.
Fig. 4 is the seed selection schematic diagram of modified form fringe excellent 128.
Embodiment
Embodiment 1: the modified form ternary hybrid rice with medium amylose content
Make up the seed selection process of five Feng You 998
Below be that example narration the present invention cultivates the implementation process that the modified form ternary hybrid rice with medium amylose content makes up five Feng You 998 with Guangzhou:
2003 early season in Guangzhou with low amylose content long-grained nonglutinous rice male sterile line five rich A respectively with have low amylose content and recover system wide extensive 998 and have high amylose content to recover wide extensive 308 production of hybrid seeds of system, obtain 2 single cross hybrid, five Feng You 998 and five Feng You 308 respectively.Summer in 2003 is in Guangzhou, and Yi Wufeng excellent 998 and five Feng You, 308 crossbreed are allotted the crossbreed of 4 mixed types by the recipe ratio of 1: 0.5,1: 0.75,1: 1 and 1: 1.25.Season in evening in 2003 was planted this mixed type crossbreed in Guangzhou.Gathered in the crops back 1 month evening in season, these 4 kinds of mixed type hybrid rice commodity paddy are carried out amylose content detects and the evaluation of rice cooking and eating quality, prove that the mixed type hybrid seed of breeding with the recipe ratio of 1: 0.5 and 1: 0.75 has medium amylose content and preferable food flavor quality.
The amylose content of table 1 five Feng You 998 and five Feng You, 308 different formulations ratios
| Two kinds of single cross hybrid recipe ratios | Amylose content % |
| 1∶0 | 13.6 |
| 0∶1 | 23.5 |
| 1∶0.5 | 17.5 |
| 1∶0.75 | 19.5 |
| 1∶1 | 20.4 |
| 1∶1.25 | 21.3 |
Embodiment 2: the modified form ternary hybrid rice with medium amylose content
The seed selection process of combination Feng You silk seedling
Adopt the male parent seed method of mass seed production by a certain percentage in Guangzhou, in 2004 early season to have the rich A in quality indica male sterile line Guangdong of low amylose content, mix male parent seed hybrid seeding with recovery system wide extensive 998 with low amylose content and recovery system wide extensive 308, bred modified form Feng You silk seedling with high amylose content.Wide extensive 998 and the mixed proportion of wide extensive 308 male parent seeds be set to 1: 0.5,1: 0.75,1: 1 and 1: 1.25.The bulk crossing kind that season in evening in 2004 produced in the production of hybrid seeds of different male parent ratio in the Guangzhou plantation.Gathered in the crops back 1 month evening in season, these 4 kinds of mixed type hybrid rice commodity paddy are carried out amylose content detects and the evaluation of rice cooking and eating quality, prove that the mixed type hybrid seed of breeding with the recipe ratio of 1: 0.5 and 1: 0.75 all has medium amylose content and preferable food flavor quality.
Table 2 male parent wide extensive 998 and wide extensive 308 different formulations ratios are to the influence of hybridization paddy amylose content
| Two kinds of male parent recipe ratios | Amylose content % |
| 1∶0 | 14.7 |
| 0∶1 | 22.5 |
| 1∶0.5 | 17.3 |
| 1∶0.75 | 19.0 |
| 1∶1 | 20.1 |
| 1∶1.25 | 21.4 |
Embodiment 3: the modified form two-line hybrid rice with medium amylose content
The seed selection process in combination Guangdong two excellent 128
Modified form Guangdong two excellent 128 of having adopted method seed selection that single cross hybrid mixes in Guangzhou.Detailed process is as follows: season morning in 2004 is to have the warm photaesthesia type nuclear sterile rice Guangdong light S of low amylose content, with wide extensive 804 hybridization of recovery system wide extensive 128 with high amylose content and recovery system, produce Guangdong two excellent 128 and Guangdong two excellent 804 two line method single cross hybrid respectively with low amylose content.Season in evening in 2004 made 4 mixed type crossbreed to Guangdong two excellent 128 and the Guangdong two excellent 804 single cross hybrid recipe ratio by 1: 0.5,1: 0.75,1: 1 and 1: 1.25 at field planting.Gathered in the crops back 1 month, and commodity paddy was carried out amylose content detect and the evaluation of rice cooking and eating quality.Proof Guangdong two excellent 128 and Guangdong two excellent 804 have medium amylose content and preferable food flavor quality by the commodity paddy of the mixed type seed output that 1: 1 to 1: 1.25 recipe ratio is produced.
The amylose content of table 3 Guangdong two excellent 128 and Guangdong two excellent 804 different formulations ratios
| Two kinds of single cross hybrid recipe ratios | Amylose content % |
| 1∶0 | 24.5 |
| 0∶1 | 14.3 |
| 1∶0.5 | 22.1 |
| 1∶0.75 | 20.6 |
| 1∶1 | 19.6 |
| 1∶1.25 | 17.3 |
Embodiment 4: the modified form ternary hybrid rice with medium amylose content
The seed selection process of combination fringe excellent 128
Adopt in Guangzhou the male parent seed by a certain percentage mass seed production the method seed selection modified form fringe excellent 128.Detailed process is as follows: early season was the rich A of wild abortion type sterile series fringe to have three of low amylose content in 2004, mix male parent seed hybrid seeding with recovery system wide extensive 128 with high amylose content and recovery system wide extensive 804, bred modified form fringe excellent 128 with low amylose content.Wide extensive 804 and the mixed proportion of wide extensive 128 male parents be set to 1: 0.5,1: 0.75,1: 1 and 1: 1.25.The bulk crossing kind that season in evening in 2004 produced in the production of hybrid seeds of different male parent ratio in the Guangzhou plantation.Gathered in the crops back 1 month evening in season, these 4 kinds of mixed type hybrid rice commodity paddy are carried out amylose content detects and the evaluation of rice cooking and eating quality, prove that the mixed type hybrid seed of breeding with the recipe ratio of 1: 0.5 and 1: 0.75 all has medium amylose content and preferable food flavor quality.
Table 4 wide extensive 804 and wide extensive 128 male parent different formulations ratios are to the influence of hybridization paddy amylose content
| Two kinds of male parent recipe ratios | Amylose content % |
| 1∶0 | 13.8 |
| 0∶1 | 24.3 |
| 1∶0.5 | 17.8 |
| 1∶0.75 | 19.4 |
| 1∶1 | 20.2 |
| 1∶1.25 | 22.4 |
Claims (9)
1, a kind of breeding method that improves hybrid rice with changed content of amylose is characterized in that, adopting 1 long-grained nonglutinous rice sterile is that maternal the recovery with 2 long-grained nonglutinous rices is paternal hybrid, production mixed type commodity hybrid seed.
2, method according to claim 1 is characterized in that, described female parent is a rice sterile line with low amylose content, and male parent is respectively the recovery system of a low amylose content and a high amylose content.
3, method according to claim 2 is characterized in that, described rice sterile line is five rich A, the rich A in Guangdong, the rich A of fringe or Guangdong light S.
4, method according to claim 2 is characterized in that: low amylose content is meant that amylose content is lower than 16%, and high amylose content is meant that amylose content is higher than 24%.
5, method according to claim 2 is characterized in that: 2 recoveries with low or high amylose content are that male parent has essentially identical form, plant and leaf shape, particle shape and close breeding time.
6, method according to claim 3, it is characterized in that: when recovering system wide extensive 998 and high amylose content and recover the wide extensive 308 bulk crossing production of hybrid seeds of system with male sterile line five rich A or the rich A in Guangdong and low amylose content, recovering system wide extensive 998 is 1: 0.5 to 1: 0.75 with wide extensive 308 male parent ratio.
7, method according to claim 3, it is characterized in that: recover system wide extensive 998 and high amylose content recovers system wide extensive 308 hybrid seedings respectively with male sterile line five rich A or the rich A in Guangdong and low amylose content, the single cross seed of producing makes the commodity-type hybrid seed in 1: 0.5 to 1: 0.75 ratio.
8, method according to claim 3, it is characterized in that: when recovering system wide extensive 804 and high amylose content and recover the wide extensive 128 bulk crossing production of hybrid seeds of system with the rich A of male sterile line fringe or Guangdong light S and low amylose content, recovering system wide extensive 804 is 1: 0.5 to 1: 1 with wide extensive 128 male parent ratio.
9, method according to claim 3, it is characterized in that: recover system wide extensive 804 and high amylose content recovers system wide extensive 128 hybrid seedings respectively with the rich A of male sterile line fringe or Guangdong light S and low amylose content, the single cross seed of producing makes the commodity-type hybrid seed in 1: 1 to 1: 1.25 ratio.
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| CNA2005100122692A CN1903013A (en) | 2005-07-28 | 2005-07-28 | Method for breeding improved hybrid rice with changed content of amylose |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102293147A (en) * | 2010-06-24 | 2011-12-28 | 云南金瑞种业有限公司 | Breeding method for hybrid rice variety |
| CN109197566A (en) * | 2018-08-10 | 2019-01-15 | 浙江大学 | A kind of selection of yellow endosperm rice |
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2005
- 2005-07-28 CN CNA2005100122692A patent/CN1903013A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102293147A (en) * | 2010-06-24 | 2011-12-28 | 云南金瑞种业有限公司 | Breeding method for hybrid rice variety |
| CN109197566A (en) * | 2018-08-10 | 2019-01-15 | 浙江大学 | A kind of selection of yellow endosperm rice |
| CN109197566B (en) * | 2018-08-10 | 2021-07-13 | 浙江大学 | Breeding method of yellow endosperm rice |
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