CN101300957A - Method for breeding Zhen dao No. 13 using ladder polymerization improved technique - Google Patents
Method for breeding Zhen dao No. 13 using ladder polymerization improved technique Download PDFInfo
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Abstract
The present invention discloses a breeding method of Zhendao 13 by step polymerization improvement technique, the rice bred by the method has the advantages of excellent quality, good taste, good rice marketability, outstanding disease resistance, good productivity and yield stability, harmonious ear-granule structure, self-adjusting ability, nice plant type and good maturing phase. The method described by the present invention comprises the following steps: A) selecting Japanese variety Guandong 135 as maternal plant to hybridize with Zhendao 2, obtaining Zhendao 72 after 4 generations inbred; B) selecting Japanese variety tsukinohikai as maternal plant to hybridize with Wuxiangjing 1, obtaining Zhendao 5088 after 4 generations inbred; C) selecting Zhendao 5088 as maternal plant to hybridize with Zhendao 72, obtaining 70 F0 seeds; D)seeding the F0 after seed soaking and pregermination, then selecting Wuyunjing 8 as maternal plant to perform multiple crossing, obtaining new rice variety namely Zhendao 13 having the advantages of high yield, good quality, stripe rust resistance and rice blast resistance.
Description
Technical field
The present invention relates to the breeding method of a plant species, more specifically to a kind of breeding method of utilizing ladder polymerization improved technique seed selection town rice No. 13.
Background technology
Paddy rice is the first crop of China, and the paddy rice of China's cultivation mainly contains two subspecies, i.e. Xian subspecies and round-grained rice subspecies.What mainly cultivated in Jiangsu is the round-grained rice subspecies, accounts for more than 90% of Jiangsu paddy rice cultivated area.SOYBEAN IN HIGH-YIELD BREEDING is the important goal of rice breeding always, and China is because populous, and arable area reduces year by year, and the pressure of grain demand strengthens, and SOYBEAN IN HIGH-YIELD BREEDING is put in the first place for a long time always, and the improvement research of rice quality lags behind.Along with the raising of people's living standard, the adjustment of pattern of farming and the pressure of competition in the international market, it is important that the quality-improving of paddy rice more shows.In recent years, change along with cropping system, stripe disease is ascendant trend year by year, the existing not anti-stripe virus disease of main breed majority, the quality of limited several anti-bar kind is also undesirable, therefore, the anti-stripe virus disease of seed selection, high-quality, high yield japonica rice kind are the important goals of Jiangsu Province's rice breeding.How breeding high-yield, withered, the blast resisting of anti-striped leaf, the japonica rice variety that has the high-quality food flavor has again become the problem that Jiangsu japonica rice breeding presses for solution.
Summary of the invention
The objective of the invention is to solve above-mentioned the deficiencies in the prior art and problem, a kind of breeding method of utilizing ladder polymerization improved technique seed selection japonica rice town rice No. 13 is provided, thereby cultivate the japonica rice new varieties of high yield, high-quality (the existing in other words good exterior quality of high-quality has good food flavor quality again), anti-stripe virus disease, blast resisting, make it obtain large scale application.
The inventor is through for many years repetition test, in many cultivation schemes and selection, find, following breeding method can well reach goal of the invention solving prior art problems, and the breeding method of ladder polymerization improved technique seed selection japonica rice town rice No. 13 of utilizing of the present invention may further comprise the steps:
A) select for use ripe phase early, high-quality (the existing in other words good exterior quality of high-quality has good food flavor quality again) in ripe in the round-grained rice Japan cultivars Northeast 135 do maternal, with No. 2 hybridization of the glutinous town of round-grained rice rice in good slow ripe of free high yield, food flavor, obtain high-quality (the existing in other words good exterior quality of high-quality has the food flavor quality of getting well again), high yield japonica rice intermediate materials town rice 72 after the selfing;
B) select for use ripe phase early, high-quality (the existing in other words good exterior quality of high-quality has good food flavor quality again), anti-stripe disease in ripe in round-grained rice Japan cultivars moonlight do maternal, with high-quality, high yield, No. 1 hybridization of the military fragrant round-grained rice of anti-pest kind, obtain ripe middle round-grained rice intermediate materials town rice 5088 in high yield, high-quality (good appearance in other words), the anti-bar after the selfing, authorization town by name rice 88;
C) select ripe intermediate keng rice intermediate materials town rice 5088 work female parents in free high yield, high-quality, the anti-bar for use, with free high-quality (the existing in other words good exterior quality of high-quality has good food flavor quality again), high yield japonica rice intermediate materials town rice 72 hybridization acquisition F
070 in seed;
D) with F
0The presoaking and germinating sowing, again with high yield, comprehensive proterties is good but the military round-grained rice of transporting of inferior kind is reestablished diplomatic relations as female parent for No. 8, allow genes such as high yield, high-quality, anti-bar, anti-rice blast progressively be aggregated to together, selfing obtains high yield, high-quality (the existing in other words good exterior quality of high-quality has good food flavor quality again), anti-bar after 6 generations, the rice blast resistant rice new varieties are promptly pressed down rice No. 13.
In the breeding method of utilizing ladder polymerization improved technique seed selection japonica rice town rice No. 13 of the present invention, described high-quality, high yield japonica rice intermediate materials town rice 72 by the ripe phase early, in the high-quality ripe in the round-grained rice Japan cultivars Northeast 135 do No. 2 hybridization of the glutinous town of round-grained rice rice in good slow ripe of maternal and high yield, food flavor also selfing obtain after 4 generations; Described town rice 5088 by round-grained rice Japan cultivars moonlight in ripe in ripe morning phase, high-quality, the anti-stripe disease do maternal and high-quality, high yield, No. 1 hybridization of the military fragrant round-grained rice of anti-pest kind also selfing obtain after 4 generations.
In the breeding method of utilizing ladder polymerization improved technique seed selection town rice No. 13 of the present invention, doing maternally to obtain F with intermediate materials town rice 5088 with 72 hybridization of intermediate materials town rice
0Behind 70 in the seed, take ladder polymerization to select the method for breeding to carry out breeding in the breeding process, described ladder polymerization improved technique is meant selects the parent material with different target proterties for use, and staged (n 〉=3) hybridization assembly one by one is with the crossbreeding mode of a plurality of excellent gene pyramidings to one.
In the breeding method of utilizing ladder polymerization improved technique seed selection town rice No. 13 of the present invention, from go on foot the poly-described selfing process of D, begin to inoculate rice blast 4 seedling stages in generation in conjunction with the anti-rice blast of whole process from selfing, high fertilizer of later stage brings out panicle blast to be identified, screens, and strengthens under the high nitrogen fertilizer adverse environmental factor simultaneously and selects the selection of pressureization to rice outward appearance and food flavor.
In the breeding method of the present invention, select ripe middle round-grained rice intermediate materials hybridization in high-quality (existing good exterior quality has good food flavor quality again), high yield japonica rice intermediate materials and high yield, high-quality (outward appearance is excellent), the anti-bar for use, and then with high yield, comprehensive proterties is good but inferior kind is reestablished diplomatic relations, allow genes such as high yield, high-quality, anti-bar, anti-rice blast progressively be aggregated to together, at CF
1Eliminate the bad individual plant of quality among the offspring and mix receipts, CF
2Plant a certain amount of colony and select individual plant to carry out system's selection in a large number; Begin artificial infection from 3 generations of selfing and identify that bacterial leaf-blight and stripe disease field natural occurrence resistance identify; Begin to inoculate rice blast 4 seedling stages in generation in conjunction with the anti-rice blast of whole process from selfing, high fertilizer of later stage brings out panicle blast and identifies, screens; When output is identified and inoculate panicle blast, in conjunction with outward appearance, food flavor quality, the material of elected better resistance.
The invention has the beneficial effects as follows:
1. best in quality, food flavor is good, and the rice commodity is good
The grains are plump is wealthy avette, clever shell stalk yellow, and commodity is good.Delivered food quality inspection center of Ministry of Agriculture test result according to 2007 by economizing the unified sampling of seed stations: brown rice yield 80.5%, polished rice rate 68.6%, head rice rate 66.7%, grain length 5.1mm, aspect ratio 1.8, the white rate 20% of chalk, chalkiness degree 3.0%, disappear 7 grades of values of alkali, glue denseness 84mm, amylose content 20.7%, 2 grades of transparencies, physics and chemistry divided 68 fens, and physical and chemical index reaches state and issues the superior rice secondary standard.Outward appearance and food flavor quality all significantly are better than No. 7, military fortune round-grained rice.
2. disease resistance is outstanding, and yield stability is good
This kind all has good resistance to bacterial leaf-blight, rice blast and stripe virus disease.Institute of Plant Protection, academy of agricultural sciences, Jiangsu Province in 2007 carries out the artificial infection evaluation with four strong pathogenic strains of Zhejiang 173, PX079, JS49-6, KS-6-6 of bacterial leaf-blight, No. 13 sick level luffings (9 grades of systems) between the 1-3 level of town rice; Seedling pest 3-5 level is identified in district's examination resistance in 2007 inoculation, and panicle blast is 3 grades; Institute of Plant Protection, academy of agricultural sciences, Jiangsu Province in 2007 carries out the field and identifies in stripe disease grave illness district, town rice No. 13 salt all, the incidence of disease of 2 of Jiangyan City is respectively 12.20%, 11.40%, anti-above level in all reaching.Field banded sclerotial blight and other mixed natural disposition diseases are light, ripe lover of later stage.No. 13 stem stalks of town rice are sturdy, than fertilizer resistance anti-lodging.
3. yielding ability is good, and the fringe kernel structure is coordinated, and self regulating power is strong
This kind tillering ability is strong, percentage of earbearing tiller height, the fringe type is bigger, and the fringe layer is neat, it is closeer grain, fringe shape half is upright, general per mu yield 600-650 kilogram (the high-yield field piece can reach more than 700 kilograms), No. 7 volume increase about 5% of the military fortune of contrast round-grained rice, about spike length 18cm, every mu of effective fringe is at 19-21 ten thousand, and every total grain panicle is counted the 120-140 grain, and ripening rate is more than 90%.About thousand kernel weight 26 grams.Self regulating power is strong, easily cultivation.
4. plant type is good, ripe lover
This product seedling low body is strong, and leaf is tall and straight, and the leaf look moderate, and tillering ability is strong, the plant type compactness, and sword-like leave is shorter, and is tall and straight, is subjected to the light attitude good, and functional period is long, and it is delicate and pretty grow, and the later stage annesl is along accent, ripe lover, about the 163 days time of infertility, it is slow ripe about 4 days that No. 7, round-grained rice is transported in tourney.
Description of drawings
Fig. 1 is a breeding method breeding pedigree schematic diagram of the present invention
Embodiment
Embodiment
1) selected for use in 1992 ripe phase early, high-quality (existing good exterior quality has good food flavor quality again) in ripe in the round-grained rice Japan cultivars Northeast 135 do maternally, hybridize for No. 2 with the glutinous town of round-grained rice rice in good slow ripe of free high yield, food flavor, get F
060 in seed is F
0Select for use ripe phase early, high-quality (existing good exterior quality has good food flavor quality again), anti-stripe disease in ripe in round-grained rice Japan cultivars moonlight do maternal, with high-quality, high yield, No. 1 hybridization of the military fragrant round-grained rice of anti-pest kind, F
0' 72 in seed is F
0';
2) 1993 with F
0And F
0' presoaking and germinating sowing, impurity elimination respectively, gather in the crops seed F
1And F
1';
3) 1994 with F
1And F
1' presoaking and germinating sowing respectively, not diseases prevention of worm is only controlled in individual plant transplanting 1200 strains, and light, the comprehensive good individual plant of proterties of disease is received in choosing, each gets 18 and 32 individual plants, i.e. F
2And F
2';
4) nineteen ninety-five is with the F that obtains
2And F
2' superseded respectively 8 strains and 14 of its outward appearance rice matter of the indoor sight of individual plant, plant 10 strains and 18 strains, according to yielding ability, aesthetic appearance screening, choosing 4 strains respectively is that 12 strains and 5 strains are 19 strains, i.e. F
3And F
3';
5) 1996 with the F that obtains
3And F
3' superseded respectively 7 strains and 9 of its outward appearance rice matter of the indoor sight of individual plant, plant 5 strains and 10 strains, at 5 strain F
3In have strain system growth prosperous, and outward appearance rice matter is fine, receives a strain and does parent (tentative town by name rice 72); At 10 strain F
3' in have strain system growth prosperous, the field shows high anti-stripe disease, and outward appearance rice matter is better, receives a strain and does parent's (called after town rice 5088, the town by name of authorization afterwards rice 88) temporarily;
6) did maternally in 1997 with ripe intermediate keng rice intermediate materials town rice in free high yield, anti-bar, the high-quality 5088, high-quality (existing good exterior quality has good food flavor quality again), 72 hybridization of high yield japonica rice intermediate materials town rice with free obtain F
080 in ' ' seed;
7) 1998 with F
0' ' presoaking and germinating sowing, again with high yield, comprehensive proterties is good but inferior kind force is transported round-grained rice and made female parent and F No. 8
0' ' reestablished diplomatic relations, and getting more than 530 in seed is CF
1
8) 1999 with CF
1Presoaking and germinating sowing, single then this to the greatest extent seedling plant, only control not diseases prevention of worm, select the individual plant that the midwifery growing way is good, disease is light, go out its outward appearance rice matter of rough sight, the eliminating of rice matter difference, it is CF that the good mixing of rice matter is reserved seed for planting
2
9) CF that mixing was reserved seed for planting in 2000
2The presoaking and germinating sowing, not diseases prevention of worm is only controlled in individual plant transplanting 1200 strains then, and it is CF that light, comprehensive good individual plant 34 strains of proterties of disease are received in choosing
3
10) CF
3Selfing: the 34 strain CF that calendar year 2001 will obtain
3Its outward appearance rice matter of indoor sight is eliminated 8 strains, plants 26 strains, and according to yielding ability, exterior quality screening, choosing 6 strains is that ripe late round-grained rice gets CF in 17 strains
4
11) CF
4Selfing: 2002 with the CF that obtains
4Plant 9 strain systems, selected 5 strains are that totally 21 strains obtain CF
5, from CF
4Bacterial leaf-blight is identified in the beginning artificial infection, carries out stripe disease natural occurrence resistance screening simultaneously;
12) CF
5Selfing: 2003 with the CF that obtains
5Planting 14 strains is, eliminates the strains system of 7 sense stripe diseases, and selected 5 strains are that totally 21 strains obtain CF
6Simultaneously from CF
4Selfing begins to inoculate rice blast seedling stage in conjunction with the anti-rice blast of whole process, and high fertilizer of later stage brings out panicle blast and identifies, screens;
13) CF
6Selfing: 2004 with the CF that obtains
6Plant 17 strain systems, eliminate 12 strain systems, in conjunction with tasting elected 2 stable strains of result, selected altogether 8 strains according to comprehensive proterties;
14) 2 stable strains will being elected in 2005 carry out the new lines approval test, the not anti-rice blast of whole process, carrying out the panicle blast inoculation heading stage identifies, wherein 1 (being numbered late mirror 15) shows as good characteristics such as high yield, anti-bar, anti-rice blast, carrying out food flavor after the results tastes, food mouthful property is better, elected continuous examination;
15) will be elected to late mirror 15 in 2006 and carry out the new lines variety comparative test, and be numbered late 2-102, and continue to show good characteristics such as high yield, anti-bar, anti-rice blast, and carry out food flavor simultaneously and taste, food mouthful property is better, recommends to participate in ripe late round-grained rice district examination in the Jiangsu Province;
16) participated in ripe late round-grained rice district examination in the Jiangsu Province, tentatively titled town rice No. 13 with town rice 662 in 2007.
Concrete breeding is through seeing Table 1.
No. 13 culture technique main points of the town rice of breeding method breeding of the present invention are as follows:
Plant experimentally through test, the output of town rice 662 per mu yield 600-650Kg constitutes: every mu of 19-21 ten thousand fringes, and every total grain panicle is several 130140, and ripening rate is more than 90%, about thousand kernel weight 27 grams.High-yield culturing should be striven sufficient fringe, attacks big fringe, and it is heavy to protect grain.Promptly by cultivating the strong seedling of many tillers, rational close planting is shortly early sent out early enough seedling and is in time put the field, improves colony's quality, accomplishes fluently the high yield basis, on the basis of guaranteeing suitable spike number, and the short strong big fringe of stalk, fringe foot, fringe are big, grain is taken back again aim of high yield thereby reach.The technical essential that should grasp is:
(1) proper seeding is cultivated strong seedling.Southern area of Jiangsu Province, sowing in about May 15, every 667m
2Rice seedling bed sowing 20-30 kilogram, the every 667m of non-irrigated seedling
2About 50 kilograms of the rice seedling bed amounts of broadcasting.Using sufficient on the basis of base manure, a leaf executes wholeheartedly that wean is fertile, and tri-leaf period, benefit was executed relay fertilizer, executed the fertilizer of standing up before the transplanting, and carried out rice seedling bed disease pest and weed preventing and controlling, the watery ricenursery length of time rice seedlings grow with about 30 days for well, the non-irrigated seedling length of time rice seedlings grow with 20-25 days for well.
(2) transplant rational close planting in good time.In go up the every 667m of fertility field piece
2Insert about 1.8 ten thousand caves basic seedling about 60,000; The relatively poor field piece of fertility is inserted 20,000 caves, basic seedling about 80,000.And accomplish an even surface planting, improve and plant quality.
(3) fertilizer application and water starch management.1., every mu of total pure nitrogen level is controlled at about the 18-20 kilogram, takes the circumstances into consideration increase and decrease according to fertility.2., heavy dressing base manure (applying organic manure), early execute short fetilizer for tillering, seedling is to putting the field raising percentage of earbearing tiller as early as possible.Using sufficient on the basis of base manure, planting the back and executed urea 10kg/ mu in 5 ~ 7 days, shallow water was diligently irritated short and was early sent out early stage, strove planting back about 20 days, and stem tiller number reaches about 200,000, light earlier later on after heavily gradation put the field, the numerical control of the highest stem tiller is built in 25 ~ 270,000.3., on the basis of middle control, early execute the short fertilizers for potted flowers of heavy dressing, short grain is striven big fringe, takes the circumstances into consideration to execute to protect fertilizers for potted flowers; Or short guarantor taken into account single-dose application; Reach and attack big fringe, strive the heavy purpose of fringe.4., final-period management to be to improve ripening rate, increase grain-weight is attached most importance to, and adopts and does wet method, must guard against and cuts off the water supply too early, it is until old ripe to guarantee to live, to guarantee high yield and high-quality.
(4) carry out the extermination of disease and insect pest.Pests occurrence rule according to disease, worm, crop smothering is in time carried out preventing and controlling.Especially note under the high-yield culturing condition,, should note carrying out the preventing and controlling of leaf-roller, paddy stem borer, rice fulgorid, rice blast, stripe virus disease and weeds because nitrogen level is higher.
The result is planted experimentally in pilot experiment and demonstration
1. result of the test and yield level
1.1 result of the test
Recommend to participate in ripe late round-grained rice regional test in the Jiangsu Province in 2007, average yield per mu is respectively 560.9 kilograms, and comparison increases production 2.2% respectively No. 7 according to military fortune round-grained rice, increase production not significantly, occupies the 8th, and ripe phase comparison was shone slow about 4 days.
1.2 yield level
General per mu yield 600-650 kilogram, the high-yield field piece can reach more than 700 kilograms.In 4 points of provinces and regions examinations time, 1 point time average yield per mu is in (2007 belong to obvious underproduction time) more than 600 kilograms, and 2 point time average yield per mus are more than 550 kilograms, and amount of increase in production is more than 2.5%.
Table 1: breeding method seed selection process of the present invention
Claims (6)
1, a kind of breeding method of utilizing ladder polymerization improved technique seed selection town rice No. 13 is characterized in that may further comprise the steps:
A) select for use ripe phase early, high-quality in ripe in the round-grained rice Japan cultivars Northeast 135 do maternally, hybridize for No. 2 with the glutinous town of round-grained rice rice in good slow ripe of free high yield, food flavor, obtain high-quality, high yield japonica rice intermediate materials town rice 72 after the selfing;
B) select for use ripe morning phase, high-quality, anti-stripe disease in ripe in round-grained rice Japan cultivars moonlight do maternal, with high-quality, high yield, No. 1 hybridization of the military fragrant round-grained rice of anti-pest kind, obtain ripe middle round-grained rice intermediate materials town rice 5088 in high yield, high-quality, the anti-bar after the selfing, authorization town by name rice 88;
C) make female parent with ripe intermediate keng rice intermediate materials town rice in free high yield, high-quality, the anti-bar 5088 again, with free high-quality, high yield japonica rice intermediate materials town rice 72 hybridization acquisition F
070 in seed;
D) with F
0The presoaking and germinating sowing, again with high yield, comprehensive proterties is good but the military round-grained rice of transporting of inferior kind is reestablished diplomatic relations as female parent for No. 8, allow genes such as high yield, high-quality, anti-bar, anti-rice blast progressively be aggregated to together, selfing obtains high yield, high-quality, anti-bar, rice blast resistant rice new varieties and promptly presses down rice No. 13 after 6 generations.
2, the breeding method of utilizing ladder polymerization improved technique seed selection town rice No. 13 according to claim 1, it is characterized in that described high-quality, high yield japonica rice intermediate materials town rice 72 by the ripe phase early, in the high-quality ripe in the round-grained rice Japan cultivars Northeast 135 do No. 2 hybridization of the glutinous town of round-grained rice rice in good slow ripe of maternal and high yield, food flavor also selfing obtain after 4 generations.
3, the breeding method of utilizing ladder polymerization improved technique seed selection town rice No. 13 according to claim 1, it is characterized in that described town rice 5088 by round-grained rice Japan cultivars moonlight in ripe in ripe morning phase, high-quality, the anti-stripe disease do maternal and high-quality, high yield, No. 1 hybridization of the military fragrant round-grained rice of anti-pest kind also selfing obtain after 4 generations.
4, the breeding method of utilizing ladder polymerization improved technique seed selection town rice No. 13 according to claim 1 is characterized in that doing maternally to obtain F with 72 hybridization of intermediate materials town rice with intermediate materials town rice 5088
0Behind 70 in the seed, take ladder polymerization to select the method for breeding to carry out breeding in the breeding process.
5, the breeding method of utilizing ladder polymerization improved technique seed selection town rice No. 13 according to claim 1, it is characterized in that from go on foot the poly-described selfing process of D, begin to inoculate rice blast 4 seedling stages in generation in conjunction with the anti-rice blast of whole process from selfing, high fertilizer of later stage brings out panicle blast to be identified, screens, and strengthens under the high nitrogen fertilizer adverse environmental factor simultaneously and selects the selection of pressureization to rice outward appearance and food flavor.
6, the breeding method of utilizing ladder polymerization improved technique seed selection town rice No. 13 according to claim 1 is characterized in that concrete steps are as follows:
(1) select for use ripe phase early, high-quality in ripe in the round-grained rice Japan cultivars Northeast 135 do maternally, hybridize for No. 2 with the glutinous town of round-grained rice rice in good slow ripe of free high yield, food flavor, get F
060 in seed is F
0Select for use ripe morning phase, high-quality, anti-stripe disease in ripe in round-grained rice Japan cultivars moonlight do maternal, with high-quality, high yield, No. 1 hybridization of the military fragrant round-grained rice of anti-pest kind, F
0' 72 in seed is F
0';
2) with F
0And F
0' presoaking and germinating sowing, impurity elimination respectively, gather in the crops seed F
1And F
1';
3) with F
1And F
1' presoaking and germinating sowing respectively, not diseases prevention of worm is only controlled in individual plant transplanting 1200 strains, and light, the comprehensive good individual plant of proterties of disease is received in choosing, each gets 18 and 32 individual plants, i.e. F
2And F
2';
4) with the F that obtains
2And F
2' superseded respectively 8 strains and 14 of its outward appearance rice matter of the indoor sight of individual plant, plant 10 strains and 18 strains, according to yielding ability, aesthetic appearance screening, choosing 4 strains respectively is that 12 strains and 5 strains are 19 strains, i.e. F
3And F
3';
5) with the F that obtains
3And F
3' superseded respectively 7 strains and 9 of its outward appearance rice matter of the indoor sight of individual plant, plant 5 strains and 10 strains, at 5 strain F
3In have strain system growth prosperous, and outward appearance rice matter is fine, receives a strain and obtains pressing down rice 72 as the parent; At 10 strain F
3' in have strain system growth prosperous, the field shows high anti-stripe disease, and outward appearance rice matter is better, receives a strain and obtains pressing down rice 5088 as the parent, the town by name of authorization afterwards rice 88;
6) make female parent with ripe intermediate keng rice intermediate materials town rice in free high yield, anti-bar, the high-quality 5088, high-quality, 72 hybridization of high yield japonica rice intermediate materials town rice with free obtain F
080 in ' ' seed;
7) with F
0' ' presoaking and germinating sowing, again with high yield, comprehensive proterties is good but inferior kind force is transported round-grained rice and made female parent and F No. 8
0' ' reestablished diplomatic relations, and getting more than 530 in seed is CF
1
8) with CF
1Presoaking and germinating sowing, single then this to the greatest extent seedling plant, only control not diseases prevention of worm, select the individual plant that the midwifery growing way is good, disease is light, go out its outward appearance rice matter of rough sight, the eliminating of rice matter difference, it is CF that the good mixing of rice matter is reserved seed for planting
2
9) CF that mixing is reserved seed for planting
2The presoaking and germinating sowing, not diseases prevention of worm is only controlled in individual plant transplanting 1200 strains then, and it is CF that light, comprehensive good individual plant 34 strains of proterties of disease are received in choosing
3
10) CF
3Selfing: the 34 strain CF that will obtain
3Its outward appearance rice matter of indoor sight is eliminated 8 strains, plants 26 strains, and according to yielding ability, exterior quality screening, choosing 6 strains is that ripe late round-grained rice gets CF in 17 strains
4
11) CF
4Selfing: with the CF that obtains
4Plant 9 strain systems, selected 5 strains are that totally 21 strains obtain CF
5, from CF
4Bacterial leaf-blight is identified in the beginning artificial infection, carries out stripe disease natural occurrence resistance screening simultaneously;
12) CF
5Selfing: with the CF that obtains
5Planting 14 strains is, eliminates the strains system of 7 sense stripe diseases, and selected 5 strains are that totally 21 strains obtain CF
6Simultaneously from CF
4Selfing begins to inoculate rice blast seedling stage in conjunction with the anti-rice blast of whole process, and high fertilizer of later stage brings out panicle blast and identifies, screens;
13) CF
6Selfing: with the CF that obtains
6Plant 17 strain systems, eliminate 12 strain systems, in conjunction with tasting elected 2 stable strains of result, selected altogether 8 strains according to comprehensive proterties;
14) 2 stable strains will being elected to carry out the new lines approval test, and the not anti-rice blast of whole process carries out the panicle blast inoculation heading stage and identifies, wherein 1 shows as good characteristics such as high yield, anti-bar, anti-rice blast, carry out food flavor after the results and taste, food mouthful property is better, elected continuous examination;
15) will be elected to late mirror 15 and carry out the new lines variety comparative test, and continue to show good characteristics such as high yield, anti-bar, anti-rice blast, and carry out food flavor simultaneously and taste, food mouthful property is better, promptly obtains pressing down rice No. 13.
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| CN103380725A (en) * | 2013-06-26 | 2013-11-06 | 盐城明天种业科技有限公司 | Cultivation method of high-quality, disease-resistance, delay-sowing-resistance and mid-season medium japonica rice breed |
| CN105063206A (en) * | 2015-08-03 | 2015-11-18 | 南京大学 | Molecular breeding method using plant anti-disease gene enriched clusters, and applications thereof |
| CN105918113A (en) * | 2016-05-20 | 2016-09-07 | 中国科学院东北地理与农业生态研究所 | Breeding method for polymerizing saline and alkaline resisting and rice blast resisting characters of rice |
| CN109924124A (en) * | 2019-04-01 | 2019-06-25 | 江苏丘陵地区镇江农业科学研究所 | A method of lasting powdery-mildew-resistance wheat breeding element is formulated based on multiple gene polymerization |
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2008
- 2008-06-18 CN CNA2008101227750A patent/CN101300957A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102388777A (en) * | 2011-07-28 | 2012-03-28 | 江苏丰源种业有限公司 | Breeding method of high-yield multi-resistance non-glutinous rice suitable for light simplified cultivation |
| CN103380725A (en) * | 2013-06-26 | 2013-11-06 | 盐城明天种业科技有限公司 | Cultivation method of high-quality, disease-resistance, delay-sowing-resistance and mid-season medium japonica rice breed |
| CN105063206A (en) * | 2015-08-03 | 2015-11-18 | 南京大学 | Molecular breeding method using plant anti-disease gene enriched clusters, and applications thereof |
| CN105918113A (en) * | 2016-05-20 | 2016-09-07 | 中国科学院东北地理与农业生态研究所 | Breeding method for polymerizing saline and alkaline resisting and rice blast resisting characters of rice |
| CN109924124A (en) * | 2019-04-01 | 2019-06-25 | 江苏丘陵地区镇江农业科学研究所 | A method of lasting powdery-mildew-resistance wheat breeding element is formulated based on multiple gene polymerization |
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