CN114711104A - Breeding method of bonsai ornamental rape - Google Patents
Breeding method of bonsai ornamental rape Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/15—Leaf crops, e.g. lettuce or spinach
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
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Abstract
The invention belongs to the field of rape breeding, and particularly relates to a method for breeding potted ornamental rape. The method adopts the lodging-resistant rape variety with short whole growth period as a parent, so that the bred dwarf variety is easy to vernalize, has the advantages of short growth period, early flowering and weak winterness, and is suitable for cultivation of potted ornamental rape flowers. The invention firstly creates short-stalk short-growth-period mutant plant types through a mutagenesis technology, and then hybridizes the obtained short-stalk mutant strains with rape varieties with different colors and large leaf color and petals, thereby simultaneously obtaining a plurality of improved varieties in one cultivation period and greatly improving the breeding efficiency. According to the invention, the potted landscape rape with flower is bred into the sterile line, so that the flowering time of the potted landscape rape flower can be prolonged, the ornamental value is improved, the pollen dispersion of indoor and scenic spots can be reduced, and the body health of the allergic residents can be protected.
Description
Technical Field
The invention belongs to the technical field of rape breeding, and particularly relates to a breeding method of a rape variety suitable for potted landscape ornamental.
Background
The rape flower is beautiful and elegant, is deeply loved by people, and therefore has wide application in rural tourism and scenic spot construction. At present, rape flowers are mainly planted in fields, but the influence of external factors on the field rape flowers is large, and the field rape flowers usually need to be driven to suburbs or rural areas to be viewed in proper weather. However, if the rape flowers can be cultivated through the bonsai, the rape flowers can be watched at any time and any place by placing the rape flowers in scenes such as homes, scenic spots, exhibition halls and the like, and therefore the entertainment life of people is greatly enriched.
However, the current rape varieties are mainly bred around the yield level, the height of the rape is about 200cm when the rape is mature, the height of the rape is about 160cm when the rape blooms, the rape has large biomass and loose inflorescence, the rape is generally suitable for field planting and large-population planting, and if the rape is directly used as potted landscape ornamental rape to be planted, the plant type is too high, the head and feet are light, the potted landscape is easy to fall down, the flowering branches are long, the flower type is dispersed, and the attractiveness is insufficient. Therefore, the prior art discloses a breeding method of rape varieties suitable for balcony cultivation, which comprises the steps of hybridizing a cabbage type rape line 167B serving as a female parent with a floral kale, and then carrying out multi-generation backcross on the hybrid progeny by taking 167B as a male parent until a selfing group with stable and consistent specific characters is obtained; and then carrying out dark culture on the seeds, reserving a young embryo with the hypocotyl shorter than 3.0cm, carrying out multi-generation selfing on a single plant with the height of less than 70cm at the initial flowering stage after culturing until obtaining homozygous and stable short-stalk selfing line seeds, namely the rape variety suitable for balcony cultivation. Although the technology overcomes the defect that the cabbage type rape is not suitable for balcony potting and urban greening, the growth period of the material flower kale used by the technology is longer than that of the traditional medium-ripened cabbage type rape, the full growth period exceeds 210 days, the flowering period is late, the kale can bloom only through strict vernalization, the adverse factors of long growth period, late flowering, strong winter and the like can be brought when the material is transferred into the rape through artificial hybridization, and the cultivation of potted ornamental rape flowers cannot be realized. In addition, the technology introduces the seedling stage ornamental property of the collard into the rape variety through distant hybridization and backcross, so that only one improved variety can be obtained in one cultivation period, the breeding efficiency is low, and the rape variety cultivated by the technology is a non-sterile line and is not friendly to pollen allergic people.
Disclosure of Invention
In view of the above, the invention mainly solves the technical problems of long growth period, late flowering and strong winter property of the existing bonsai ornamental rape.
The invention aims to solve another technical problem of how to obtain a plurality of improved varieties simultaneously in one cultivation period so as to improve the breeding efficiency.
The purpose of the invention is realized by the following technical scheme:
a method for breeding ornamental rape in miniascape comprises the following steps:
(1) selecting an anti-lodging rape variety with a full growth period of 180-210 days as a parent, and carrying out mutagenesis treatment on the seed of the parent to obtain a mutagenized seed; planting the mutagenized seeds, selfing the single plants after flowering, and harvesting M1 generation seeds after maturation;
(2) planting the M1 seeds, selecting a single plant with the plant height of 30-60cm for selfing in the flowering period, selecting a weak winter early-maturing short stalk mutant single plant with the plant height of 30-100cm in the mature period and the full growth period of less than 180 days, and harvesting M2 seeds;
(3) planting the M2 generation seeds, and harvesting M3 generation seeds according to the method in the step (2);
(4) planting seeds of the M3 generation, hybridizing the seeds with rape varieties with different flower colors and leaf colors and large petals respectively in the flowering period, backcrossing for 2-3 generations to obtain short-stalk single plants with different flower colors and leaf colors and large petals, planting seeds of the short-stalk single plants, selfing for 5-6 generations to obtain short-stalk homozygous lines with different leaf colors and flower colors and large petals.
Optionally, the parent is double 11 or early-maturing variety gan Zao oil No. 1 in the middle-maturing variety.
Optionally, the mutagenic treatment is performed by irradiation with one or more of, but not limited to, X-rays, gamma rays, 60Co gamma rays, cosmic rays, atmospheric pressure, room temperature plasma based on the atmospheric pressure injection glow discharge principle.
Optionally, a mutagen is used for the mutagenesis treatment, specifically: soaking the parent seeds for 8 hours at 30 ℃, and then soaking the parent seeds in a solution with the mass percentage of Ethyl Methanesulfonate (EMS) of 1.5% for 24 hours. The mutagenesis efficiency of the method can reach about 0.1 percent, and short-stalk premature mutants are easy to screen.
Optionally, the mutagenized seed is planted in a test field having segregating conditions.
Optionally, the test field is not surrounded by cruciferous plants, such as oilseed rape. Sowing is generally carried out from the last 9 months to the last 10 months. The fertilizer application in the field is carried out according to the principle of heavy base fertilizer application, increased phosphorus-potassium fertilizer application and necessary boron fertilizer application, wherein 10-12kg of pure nitrogen and phosphorus (P) are applied per mu2O5)5-6kg, potassium (K)2O)5-6kg and borax 1.0-1.5 kg. Wherein, nitrogen fertilizer is applied at 60% base, and seedling fertilizer and bolting fertilizer are respectively applied at 20%; completely applying the phosphate fertilizer at the bottom; 70% of potassium fertilizer is applied on the bottom, and 30% of potassium fertilizer is used as a bolting fertilizer; 0.9-1.35kg of borax is applied at the bottom, and 150g of leaves at the bolting stage are sprayed. After the base fertilizer is applied to the field, the rape seeder (model: 2BYD-6, no seeds are added) is used for completing the operations of plowing, ditching, compartment making and the like of the test field at one time, the width of the compartment is 1.5m, and the uniform seeding and the tidy seedling emergence are ensured. After flowering, all the individual plants are bagged by sulfuric acid paper bags or non-woven fabric bags according to the bagging requirement of rape breeding so as to realize individual plant selfing.
Optionally, in the step (4), the rape variety is a purple-leaf and large-petal red-flower rape variety YC102, a purple-leaf and large-petal white-flower rape variety YC103, a green-leaf and large-petal red-flower rape variety YC104 or a green-leaf and large-petal white-flower rape variety YC105 which are bred by self-crossing of orange-red-flower rape CS3 and purple-flower rape CS4 (see "color rape variety and application thereof", Zhang Dong et al, Zhejiang agricultural science 2015, 56(9):1442 and 1443) provided by Zhejiang acan agricultural academy of sciences; the specific breeding method comprises the following steps: hybrid seeds F are obtained by hybridizing the flowering of orange flower rape CS3 with purple flower rape CS41Hybrid seed F1Continuously bagging and selfing for 5 generations, and selecting purple-leaf large-petal safflower single plant, purple-leaf large-petal white flower single plant and green-leaf large-petal safflower single plant from separated progenyAnd respectively harvesting seeds of the individual plants, planting the seeds, continuously bagging and selfing for 1-3 generations to obtain rape varieties YC102, YC103, YC104 and YC 105.
Optionally, the breeding method further comprises the step of transforming the dwarf homozygous lines with different leaf colors and flower colors and large petals obtained in the step (4) into sterile lines.
Optionally, the transformation method of the sterile line comprises: taking the short stalk homozygous lines with different leaf colors and flower colors and large petals obtained in the step (4) as male parents, selecting cytoplasmic sterile plants with large petals as female parents, and hybridizing to obtain F1 generation sterile plants; and backcrossing for a plurality of generations by taking the F1 generation sterile plant as a female parent until a short-stalk sterile line with different leaf colors and flower colors and large petals is bred.
Alternatively, the different leaf colors and flower colors may be, for example, red flower of purple leaf, white flower of purple leaf, red flower of green leaf, white flower of green leaf. The big petals mean that the diameter of each flower is more than 16 mm.
A rape flower pot culture comprises rape seeds obtained by the breeding method.
The rape flower potted plant provided by the invention comprises a flowerpot, nutrient soil and rape seeds obtained by the breeding method. The potted plant can be a commercial product, after the consumer purchases the potted plant, the rape seeds are planted in the nutrient soil in the flowerpot according to the guidance of the cultivation instruction, water is applied, and the seeds can germinate and bloom after a plurality of days to grow into beautiful potted rape flowers.
Compared with the prior art, the technical scheme of the invention has the following advantages:
1. the breeding method of the potted ornamental rape provided by the embodiment of the invention adopts the lodging-resistant rape variety with short full-growth period (180-210 days) as a parent, so that the bred dwarf variety is easy to vernalize, has the advantages of short growth period, early flowering and weak winter property, and is suitable for cultivation of potted ornamental rape flowers. In addition, the breeding method firstly creates short-stalk short-growth-period mutant plant types through a mutagenesis technology, and then the obtained short-stalk mutant strains are hybridized with rape varieties with different colors and large leaf color petals, so that a plurality of improved varieties can be obtained simultaneously in one cultivation period, and the breeding efficiency is greatly improved.
2. According to the breeding method of the potted ornamental rape provided by the embodiment of the invention, the potted ornamental rape is transformed into the sterile line, so that the flowering time of potted ornamental rape flowers can be prolonged, the ornamental value is improved, the pollen dispersion in rooms and scenic spots can be reduced, and the body health of allergic inhabitants can be protected.
Drawings
FIG. 1 is a comparison graph of the appearance of the mutant dwarfed strains obtained by mutagenesis in example 1 of the present invention and the plant of double No. 11 in the parent.
Detailed Description
The following examples are provided to further understand the present invention, not to limit the scope of the present invention, but to provide the best mode, not to limit the content and the protection scope of the present invention, and any product similar or similar to the present invention, which is obtained by combining the present invention with other prior art features, falls within the protection scope of the present invention.
The examples do not show the specific experimental steps or conditions, and can be performed according to the conventional experimental steps described in the literature in the field. The reagents or instruments used are not indicated by manufacturers, and are all conventional reagent products which can be obtained commercially.
The invention provides a method for breeding potted ornamental rape, which comprises the following steps:
1. creation of short-stalk rape
1.1 mutagenesis of oilseed rape varieties
Selecting a rape variety with strong disease resistance and lodging resistance and short full-growth period (180-210 days), such as a double 11 or early-maturing variety Ganxiao oil No. 1 in a medium-maturing variety as a parent, and carrying out mutagenesis treatment on seeds of the parent by adopting a physical radiation method or a chemical method, wherein the physical radiation method comprises but is not limited to adopting X rays, gamma rays, 60Co gamma rays, cosmic rays and normal-pressure room-temperature plasma (ARTP) based on an atmospheric pressure injection frequency glow discharge principle as a radiation source; the chemical method takes EMS (ethyl methane sulfonate) as a mutagen, and specifically comprises the following steps: rape seeds are soaked for 8 hours at the temperature of 30 ℃, and then are soaked for 24 hours in 1.5 percent by mass of Ethyl Methanesulfonate (EMS) solution, the mutagenesis efficiency can reach about 0.1 percent, and short-stalk premature mutants can be easily screened.
All mutagenic seed resources are planted in the test field with isolated conditions according to the conventional cultivation technology, and are generally sown in the last 9-10 months. The fertilizer application in the field is carried out according to the principle of heavy base fertilizer application, increased phosphorus-potassium fertilizer application and necessary boron fertilizer application, wherein 10-12kg of pure nitrogen and phosphorus (P) are applied per mu2O5)5-6kg, potassium (K)2O)5-6kg, borax 1.0-1.5 kg; wherein, nitrogen fertilizer is applied at 60% base, and seedling fertilizer and bolting fertilizer are respectively applied at 20%; completely applying the phosphate fertilizer at the bottom; 70% of potassium fertilizer is applied on the bottom, and 30% of potassium fertilizer is used as a bolting fertilizer; 0.9-1.35kg of borax is applied at the bottom, and 150g of leaves at the bolting stage are sprayed. After the base fertilizer is applied to the field, the rape seeder (model: 2BYD-6, no seeds are added) is used for completing the operations of plowing, ditching, compartment making and the like of the test field at one time, the width of the compartment is 1.5m, and the uniform seeding and the tidy seedling emergence are ensured. After flowering, all the single plants are bagged by sulfuric acid paper bags or non-woven fabric bags according to the bagging requirement of rape breeding, and after the seeds are mature, all the single plants M1 generation seeds are harvested.
1.2 Material identification
The harvested M1 generation seeds are all sown according to the cultivation method, a single plant with the plant height of 30-60cm is selected in the flowering period for bagging and selfing, and a weak winter early-maturing short stalk mutation single plant with the height of 30-100cm and the full growth period of less than 180 days is selected in the maturation period to harvest the M2 generation seeds.
And (3) carrying out identification on the seeds of the M2 generation in the second year according to the method, selecting a dwarf line with stable phenotype, harvesting seeds of the M3 generation, and applying the seeds to the breeding of potted ornamental rape varieties.
2. Polymerization of different flower color, leaf color, large petal and short stalk
2.1 selecting rape varieties with different flower colors, leaf colors and large petals (such as red-leaf large-petal safflower, red-leaf large-petal white flower, green-leaf large-petal safflower, green-leaf large-petal white flower and the like), hybridizing with the created rape resources with short whole growth period (less than 180 days) and short whole growth period (30-100cm), and continuously backcrossing for 2-3 generations.
2.2 selecting and polymerizing short stalk single plants with different leaf colors, different flower colors and large petals for selfing for 5-6 generations until breeding short stalk homozygous lines with different leaf colors, different flower colors and large petals.
3. Transformation of large and short stem sterile line with different leaf color, different flower color and petal
The transformation of the potted landscape rape into the sterile line can prolong the flowering time of potted landscape rape flowers, improve the ornamental value, reduce the pollen dispersion in the indoor and scenic spots and protect the health of allergic people.
3.1 selecting cytoplasmic sterile plants with large petals (the diameter of the flower is more than 16mm) as female parents, and using the short-stalk homozygous single plants with different leaf colors, different flower colors and large petals obtained in the step 2 as male parents to perform hybridization to obtain hybrid F1 generation sterile plants.
3.2 taking the sterile plant of the hybrid F1 generation as a female parent, taking the short stalk homozygous single plant with different leaf colors, different flower colors and large petals obtained in the step 2 as a male parent, and hybridizing again for 5 generations until the short stalk large flower sterile line with different leaf colors and different flower colors is bred.
The breeding method of the bonsai ornamental rape provided by the embodiment of the invention adopts the lodging-resistant rape variety with short full-growth period (180-210 days) as a parent, so that the bred short-stalk variety is easy to vernalize, and the cultivation of the four-season ornamental rape flower can be realized. In addition, the breeding method firstly creates short-stalk short-growth-period mutant plant types through a mutagenesis technology, and then the obtained short-stalk mutant strains are hybridized with rape varieties with different colors and large leaf color petals, so that a plurality of improved varieties can be obtained simultaneously in one cultivation period, and the breeding efficiency is greatly improved.
Example 1
The invention provides a method for breeding potted ornamental rape, which comprises the following steps:
1. creation of short-stalk rape
1.1 mutagenesis of oilseed rape varieties
Selecting double No. 11 in the medium-maturing variety with strong disease resistance and lodging resistance and short full-growth period (180-210 days) as a parent, and performing mutagenesis treatment on the seed of the parent by adopting an EMS solution, wherein the method specifically comprises the following steps: soaking the parent seeds for 8 hours at 30 ℃, and then soaking the parent seeds in 1.5 mass percent Ethyl Methane Sulfonate (EMS) solution for 24 hours;
all mutagenic seed resources are planted in the test field with isolated conditions according to the conventional cultivation technology, and are generally sown in the last 9-10 months. The fertilizer application in the field is carried out according to the principle of heavy base fertilizer application, increased phosphorus-potassium fertilizer application and necessary boron fertilizer application, wherein 10-12kg of pure nitrogen and phosphorus (P) are applied per mu2O5)5-6kg, potassium (K)2O)5-6kg, borax 1.0-1.5 kg; wherein, nitrogen fertilizer is applied at 60% base, and seedling fertilizer and bolting fertilizer are respectively applied at 20%; completely applying the phosphate fertilizer at the bottom; 70% of potassium fertilizer is applied on the bottom, and 30% of potassium fertilizer is used as a bolting fertilizer; 0.9-1.35kg of borax is applied at the bottom, and 150g of leaves at the bolting stage are sprayed. After the base fertilizer is applied to the field, the rape seeder (model: 2BYD-6, no seeds are added) is used for completing the operations of plowing, ditching, compartment making and the like of the test field at one time, the width of the compartment is 1.5m, and the uniform seeding and the tidy seedling emergence are ensured. After flowering, all the single plants are bagged by sulfuric acid paper bags or non-woven fabric bags according to the bagging requirement of rape breeding, and after the seeds are mature, all the single plants M1 generation seeds are harvested.
1.2 Material identification
The harvested M1 generation seeds are all sowed according to the cultivation method, a single plant with the plant height of 30-60cm is selected in the flowering period to be bagged and selfed, and a weak winter early-maturing short-stalk mutation single plant with the height of 30-100cm and the full growth period of less than 180 days is selected in the maturation period to harvest M2 generation seeds.
And (3) carrying out identification on the seeds of the M2 generation in the second year according to the method, selecting a dwarf line with stable phenotype as shown in figure 1, harvesting seeds of the M3 generation, and applying the seeds to the breeding of potted ornamental rape varieties.
2. Polymerization of purple leaf large-petal safflower and dwarf stalk
2.1 selecting purple-leaf large-petal safflower rape variety YC102 and short-stem (30-100cm) rape resource hybridization with short full-growth period (less than 180 days), and continuously backcrossing for 2-3 generations.
2.2 selecting short stalk single plant polymerized with purple leaf, safflower and large petal, selfing for 5-6 generations until breeding into short stalk homozygous plant polymerized with purple leaf, large petal and safflower.
3. Transformation of sterile line
3.1 selecting cytoplasmic sterile plant with large petals (the diameter of the flower is more than 16mm) as female parent, and using the short-stalk homozygous single plant of purple leaf, large petal and safflower obtained in the step 2 as male parent, and making hybridization to obtain hybrid F1 generation sterile plant.
3.2 taking the sterile plant of the hybrid F1 generation as a female parent, taking the short-stalk homozygous single plant of the purple leaves, the big petals and the safflower obtained in the step 2 as a male parent, and hybridizing again for 5 generations until the short-stalk big-flower sterile line of the purple leaves, the big petals and the safflower is selected and bred.
Example 2
The contents are the same as those of example 1 except for the following.
The purple-leaf large-petal white-flower rape variety YC103 and the created short-stalk (30-100cm) rape resource with short full-growth period (less than 180 days) are selected for hybridization.
Example 3
The contents are the same as those of example 1 except for the following.
The green leaf big petal safflower rape variety YC104 is selected to be hybridized with the rape resource which is created with short stalk (30-100cm) and short whole growth period (less than 180 days).
Example 4
The contents are the same as those of example 1 except for the following.
The green-leaf large-petal white-flower rape variety YC105 is selected to be hybridized with the rape resource which is created with short stalk (30-100cm) and short whole growth period (less than 180 days).
The data of the obtained dwarf mutant strain ornamental rape and the Zhongshuang No. 11 parent strain are compared and shown in the table 1.
TABLE 1
It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications therefrom are within the scope of the invention.
Claims (10)
1. A method for breeding ornamental rape bonsai is characterized by comprising the following steps:
(1) selecting an anti-lodging rape variety with a full growth period of 180-210 days as a parent, and carrying out mutagenesis treatment on the seed of the parent to obtain a mutagenized seed; planting the mutagenized seeds, selfing the single plants after flowering, and harvesting M1 generation seeds after maturation;
(2) planting the M1 seeds, selecting a single plant with the plant height of 30-60cm for selfing in the flowering period, selecting a single plant with the plant height of 30-100cm in the mature period and the full growth period of less than 180 days, and harvesting M2 seeds;
(3) planting the M2 generation seeds, and harvesting M3 generation seeds according to the method in the step (2);
(4) planting seeds of the M3 generation, hybridizing the seeds with rape varieties with different flower colors and leaf colors and large petals respectively in the flowering period, backcrossing for 2-3 generations to obtain short-stalk single plants with different flower colors and leaf colors and large petals, planting seeds of the short-stalk single plants, selfing for 5-6 generations to obtain short-stalk homozygous lines with different leaf colors and flower colors and large petals.
2. The selective breeding method of claim 1, wherein the parents are double 11 rape or early-maturing rape Ganxiao oil No 1 in the medium-maturing variety.
3. A selective breeding method according to claim 1 or 2, characterized in that the mutagenic treatment is carried out by means of radiation, the radiation source including but not limited to one or more of X-rays, gamma-rays, 60Co gamma-rays, cosmic rays, atmospheric pressure room temperature plasma based on the atmospheric pressure injection frequency glow discharge principle.
4. The breeding method according to claim 1 or 2, characterized in that the mutagenic treatment is carried out using a mutagenizing agent, in particular: soaking the parent seeds for 8 hours at 30 ℃, and then soaking the parent seeds in a solution with the mass percentage of ethyl methanesulfonate of 1.5% for 24 hours.
5. The breeding method according to any one of claims 1 to 4, wherein the mutagenized seeds are planted in a test field with segregating conditions.
6. The breeding method according to claim 5, wherein crucifers cannot be present around the test field.
7. The breeding method according to any one of claims 1 to 6, wherein the rape variety in step (4) is a purple-leaf and large-petal rape variety YC102, a purple-leaf and large-petal white-flower rape variety YC103, a green-leaf and large-petal safflower rape variety YC104, or a green-leaf and large-petal white-flower rape variety YC105, which is bred by crossing the red-flower rape CS3 and the purple-flower rape CS4 provided by Zhejiang academy of agricultural sciences.
8. The breeding method according to any one of claims 1 to 7, further comprising transforming the dwarf homozygous lines with different leaf colors and flower colors and large petals obtained in step (4) into sterile lines.
9. The breeding method of claim 8, wherein the transformation method of the sterile line comprises: taking the short stalk homozygous lines with different leaf colors and flower colors and large petals obtained in the step (4) as male parents, selecting cytoplasmic sterile plants with large petals as female parents, and hybridizing to obtain F1 generation sterile plants; and backcrossing for a plurality of generations by taking the F1 generation sterile plant as a female parent until a short-stalk sterile line with different leaf colors and flower colors and large petals is bred.
10. A potted canola plant comprising canola seeds obtained by the selective breeding method of any one of claims 1-9.
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| CN116326474A (en) * | 2023-03-15 | 2023-06-27 | 信阳市农业科学院 | Breeding method of high-yield stress-resistant short-stalk new variety rape |
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| CN111149682A (en) * | 2020-01-02 | 2020-05-15 | 贵州省油料研究所(贵州省香料研究所) | Breeding method of colorful leaf dwarfing ornamental rape line |
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| CN111149682A (en) * | 2020-01-02 | 2020-05-15 | 贵州省油料研究所(贵州省香料研究所) | Breeding method of colorful leaf dwarfing ornamental rape line |
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| 孙其信主编: "作物育种学", vol. 1, 中国农业大学出版社, pages: 89 * |
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| CN116326474A (en) * | 2023-03-15 | 2023-06-27 | 信阳市农业科学院 | Breeding method of high-yield stress-resistant short-stalk new variety rape |
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