CN1799333A - High-yield seeds production technique for japonica hybrid rice - Google Patents

High-yield seeds production technique for japonica hybrid rice Download PDF

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Publication number
CN1799333A
CN1799333A CNA2005101308862A CN200510130886A CN1799333A CN 1799333 A CN1799333 A CN 1799333A CN A2005101308862 A CNA2005101308862 A CN A2005101308862A CN 200510130886 A CN200510130886 A CN 200510130886A CN 1799333 A CN1799333 A CN 1799333A
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parent
time
male parent
days
blooming
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CN100391331C (en
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张忠旭
华泽田
李全英
王颜荣
郝宪彬
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LIAONING RICE RESEARCH INSTITUTE
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LIAONING RICE RESEARCH INSTITUTE
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Abstract

A technique of preparing seed for round-grained hybrid rice with high productivity, with the course comprising: designing blooming-time, constructing group of high productivity, forecasting blooming-time, adjusting blooming-time and artificial pollination. The method solves problems of low seed production and high cost of seed thoroughly comparing with traditional method. The course from seed culturing to blooming can be monitored, adjusted in any time, and the adjusting time is longer.

Description

High-yield seeds production technique for japonica hybrid rice
(1) technical field
The invention belongs to the technology of Japonica Hybrid production of hybrid seeds aspect.This technology relates to the content of aspects such as florescence design, prediction of flowering period, florescence adjusting, artificial pollination technology.
(2) technical background
China's hybridization water oceangoing ship area under cultivation accounts for national paddy rice cultivated area about 50%, compare with the conventional rice kind, hybrid rice generally can be increased production about 10%, the development of hybrid rice and promote has been acknowledged as China paddy rice in modern age and has bred a important milestone on the history, but, paddy rice is the same with other crop, heterotic power, caused genetic variation and genetic differentiation level in parental gene, therefore yield level does not have breakthrough, japonica hybrid rice particularly, since realizing three series mating the seventies, hybrid seed yield is low, the problem that seed costs is high just is one of key factor of its large tracts of land development of restriction always, along with agricultural to high yield, efficiently, the high-quality direction develops, and particularly China is faced with the people and increases the serious situation that soil subtracts, and guarantee to improve the needs that total grain output adapts to development, seed selection has the superelevation yield level, and adaptability is strong, the new combination of the hybrid rice of disease-resistant high-quality has become the great brainstorm subject on the rice breeding.
(3) summary of the invention
The object of the present invention is to provide a kind of high-yield seeds production technique for japonica hybrid rice, it is low that this technology has fundamentally solved hybrid seed yield, the problem that seed costs is high.
The technical scheme that adopts is:
High-yield seeds production technique for japonica hybrid rice, its feature comprises following process:
One, florescence design
Rationally arrange to broadcast this flower synchronization of the phase of inserting assurance father and mother and guarantee that blooming stage misses rainy season;
Determine parents' duration from seeding to heading according to interval sowing test over the years;
The parent long according to duration from seeding to heading determines to guarantee its sowing time that blooming stage misses rainy season;
According to definite sowing time separately of duration from seeding to heading of interval sowing test over the years and parents;
Before the rice transplanting father and mother are originally carried out the leaf age mark and determine transplanting date according to the leaf age difference;
Two, make up high yield colony
The light seeding kind is cultivated the strong seedling of band tiller;
According to male parent loose powder characteristics, determine row ratio rationally, be generally 1: 6;
The science water and fertilizer management makes up the high yield structure;
Three, florescence prediction
The leaf age follow-up investigation is carried out in the reasonable reconnaissance in field, and investigation in every 5-7 days once;
In time gather investigation result, determine that the leaf age of each investigation is poor;
With reference to leaf age investigation records over the years, comparison leaf age developmental process finds differences and in time adjusts;
Enter the ear differentiation after date, carried out an ear differentiation process and investigate in every 2--3 days;
With reference to ear differentiation process investigation records over the years, compare young fringe developmental process, find differences and in time adjust;
Four, the florescence is regulated
1, fertilizer is regulated
Before three phases of ear differentiation, find differences and to use liquid manure to regulate;
To growing the inclined to one side nitrogen fertilizer application of parent early, maternal 7--10 kilogram/mu, male parent 2-3 kilogram/mu generally can be postponed out fringe 3-5 days.
To growing slow parent's foliage-spray potassium dihydrogen phosphate 2-3 time, mu is used the 150--200 gram, converts the water kilogram, generally can go out fringe in advance about 2 days.
2, moisture is regulated
General recover system to the reaction of water than male sterile line sensitivity;
Early late female as the father, can dry field, the developmental process of control male parent;
Early the father is slow as mother, can deep irrigation, promote the developmental process of male parent;
3, hormone regulation
After three phases of ear differentiation, find differences and to use hormone to regulate;
Under the little situation of difference, can suitably delay the operating period of Activol.Generally, slow partially parent is used Activol 1 gram, convert 50 kilograms of foliage-sprays of water, can ear in advance about 2 days in the ear differentiation 6-7 phase.
As differ greatly, can spray once in per two days, spray continuously three times, can go out in advance fringe 4--5 days.
Five, artificial pollination technology
Artificial supplementary pollination is the major measure that improves outcrossing seed-setting rate.
Follow to the direction walking with 3 meters long bamboo bars, impel pollen to soar with the bamboo bar is round in the middle part of paternal plant gently by heavily pushing away, wide loosing.From the later parent of blooming bloom the number reach 30%, caught up with powder once in every 30---45 minute, every day 3--4 time.
With 5 meters long bamboo bar, hold bamboo bar middle part and walk to direction along vertical row, gently impel pollen to soar with the bamboo bar, wide loosing by heavily pushing away male parent.From the later parent of blooming bloom the number reach 30%, caught up with powder once in every 30--45 minute, every day 3--4 time.
The large tracts of land production of hybrid seeds adopts the stay cord method to pollinate.The nylon rope of two humans fingers thickness is jogged to direction along vertical row, makes rope scrape male parent, and the male parent bounce-back is kicked up pollen is high, reaches the purpose of pollination.
This method and conventional method compare, and it is low fundamentally to have solved hybrid seed yield, the problem that seed costs is high., can whole process monitor at any time until blooming from the beginning of growing seedlings, regulate at any time, the time of adjusting is more abundant.
(4) embodiment
Embodiment one
Adopting said method in 2004 is excellent 1052 in Liaoyang production of hybrid seeds the Liao Dynasty, and 400 mu of final average hybrid seed yields of area reach 265 kilograms/mu.
1, kind is selected for use
Carry out the production of hybrid seeds, kind selects for use the Liao Dynasty excellent 1052, male parent C52 duration from seeding to heading 110 days, maternal 105A duration from seeding to heading 105 days.The male parent duration from seeding to heading is longer, and in conjunction with Liaoyang local climate situation, overcast and rainy season, later sowing can keep away rainy season April 20 can to determine male parent in the last ten-days period in July.According to this duration from seeding to heading of father and mother,, in the late 10 days sowing time that can determine female parent, sowed April 30 than male parent in conjunction with interval sowing test over the years.Mark leaf age before the rice transplanting, during rice transplanting the leaf age of male parent than the big a slice leaf of female parent about.
Want light seeding when 2, sowing, every square metre of sowing quantity can not surpass 200 grams.Bigger according to the paternal pollen amount, determine row ratio rationally, be 1: 6;
The science water and fertilizer management makes up the high yield structure.
3, prediction of flowering period
According to the concrete condition in local field, 8 points for investigation are set, carry out the leaf age follow-up investigation, in conjunction with interval sowing test data over the years,, developmental process is monitored with reference to the leaf age difference of different times, pinpoint the problems, in time adjust.
Enter and carry out young fringe behind the panicle spike primordium differentiation stage and broadcast and look into, before five phases, male parent ear differentiation is than the Zao first phase of female parent.The maternal ear differentiation process of five after dates is accelerated, and drives male parent gradually away.
4, when the production of hybrid seeds of Liaoyang, male parent ear differentiation is during five phases, and maternal ear differentiation also reached for five phases, and convert water 50 kilogram with Activol one gram this moment, sprays male parent, once connects twice of spray every other day.Father and mother originally go out fringe simultaneously.
5, pollination situation is regulated
When father and mother originally go out fringe 5%, earlier male parent is sprayed Activol 0.5 gram/mu, full field sprayed Activol 1 gram/mu in second day.Can guarantee the male parent plant height like this than maternal high 10-20 centimetre, it is neat to make father and mother originally go out fringe again, thereby improves outcrossing seed-setting rate.
6, artificial pollination
Adopt the stay cord method to pollinate.Male parent is bloomed about 10:40 normal every day, and bloom about 10:35 maternal normal every day, and flowering time is almost synchronous.Bloom the peak at 11:30 to 12:00.From male parent bloom the number reach 30%, caught up with powder once in every 30-45 minute, every day 3-4 time.
Embodiment two
Adopting said method in 2004 is excellent 9952 in Liaoyang production of hybrid seeds the Liao Dynasty, and 200 mu of final average hybrid seed yields of area reach 245 kilograms/mu.
1, kind is selected for use
Kind selects for use the Liao Dynasty excellent 9952, male parent C52 duration from seeding to heading 110 days, maternal 99A duration from seeding to heading 110 days.The male parent duration from seeding to heading is identical, and in conjunction with Liaoyang local climate situation, can determine that in the last ten-days period in July originally later sowing can keep away rainy season father and mother April 20 overcast and rainy season.According to father and mother's duration from seeding to heading originally,, can determine that father and mother are identical this sowing time in conjunction with interval sowing test over the years.Mark leaf age before the rice transplanting, father and mother's leaf age originally is basic identical during rice transplanting.
Want light seeding when 2, sowing, every square metre of sowing quantity can not surpass 200 grams.Bigger according to the paternal pollen amount, determine row ratio rationally, be 1: 6;
The science water and fertilizer management makes up the high yield structure.
3, prediction of flowering period
According to the concrete condition in local field, 10 points for investigation are set, carry out the leaf age follow-up investigation, in conjunction with interval sowing test data over the years,, developmental process is monitored with reference to the leaf age difference of different times, pinpoint the problems, in time adjust.
Enter and carry out young fringe behind the panicle spike primordium differentiation stage and broadcast and look into, before five phases, male parent ear differentiation is than the Zao semiduation of female parent.The maternal ear differentiation process of six after dates is accelerated, and drives male parent gradually away.
4, pollination situation is regulated
When father and mother originally go out fringe 5%, earlier male parent is sprayed Activol 0.5 gram/mu, full field sprayed Activol 1 gram/mu in second day.Can guarantee the male parent plant height like this than maternal high 10-20 centimetre, it is neat to make father and mother originally go out fringe again, thereby improves outcrossing seed-setting rate.
5, artificial pollination
Adopt the stay cord method to pollinate.Male parent is bloomed about 10:40 normal every day, and bloom about 10:35 maternal normal every day, and flowering time is almost synchronous.Bloom the peak at 11:30 to 12:00.From male parent bloom the number reach 30%, caught up with powder once in every 30-45 minute, every day 3-4 time.
Example example three
Adopting said method in 2005 is excellent 1052 in Liaoyang production of hybrid seeds the Liao Dynasty, and 400 mu of final average hybrid seed yields of area reach 270 kilograms/mu.
1, kind is selected for use
Kind selects for use the Liao Dynasty excellent 1052, male parent C52 duration from seeding to heading 110 days, maternal 105A duration from seeding to heading 105 days.The male parent duration from seeding to heading is longer, and in conjunction with Liaoyang local climate situation, overcast and rainy season, later sowing can keep away rainy season April 20 can to determine male parent in the last ten-days period in July.According to this duration from seeding to heading of father and mother,, in the late 10 days sowing time that can determine female parent, sowed April 30 than male parent in conjunction with interval sowing test over the years.Mark leaf age before the rice transplanting, during rice transplanting the leaf age of male parent than the big a slice leaf of female parent about.
Want light seeding when 2, sowing, every square metre of sowing quantity can not surpass 200 grams.Bigger according to the paternal pollen amount, determine row ratio rationally, be 1: 6.
The science water and fertilizer management makes up the high yield structure.
3, prediction of flowering period
According to the concrete condition in local field, 10 points for investigation are set, carry out the leaf age follow-up investigation, in conjunction with interval sowing test data over the years,, developmental process is monitored with reference to the leaf age difference of different times, pinpoint the problems, in time adjust.
Enter and carry out young fringe behind the panicle spike primordium differentiation stage and broadcast and look into, before five phases, male parent ear differentiation is than the Zao first phase of female parent.The maternal ear differentiation process of five after dates is accelerated, and drives male parent gradually away.
4, pollination situation is regulated
When father and mother originally go out fringe 5%, earlier male parent is sprayed Activol 0.5 gram/mu, full field sprayed Activol 1 gram/mu in second day.Can guarantee the male parent plant height like this than maternal high 10-20 centimetre, it is neat to make father and mother originally go out fringe again, thereby improves outcrossing seed-setting rate.
5, artificial pollination
Adopt the stay cord method to pollinate.Male parent is bloomed about 10:40 normal every day, and bloom about 10:35 maternal normal every day, and flowering time is almost synchronous.Bloom the peak at 11:30 to 12:00.From male parent bloom the number reach 30%, caught up with powder once in every 30-45 minute, every day 3-4 time.

Claims (1)

1, high-yield seeds production technique for japonica hybrid rice, its feature comprises following process:
A, florescence design
Rationally arrange to broadcast this flower synchronization of the phase of inserting assurance father and mother and guarantee that blooming stage misses rainy season;
Determine parents' duration from seeding to heading according to interval sowing test over the years;
The parent long according to duration from seeding to heading determines to guarantee its sowing time that blooming stage misses rainy season;
According to definite sowing time separately of duration from seeding to heading of interval sowing test over the years and parents;
Before the rice transplanting father and mother are originally carried out the leaf age mark and determine transplanting date according to the leaf age difference;
B, structure high yield colony
The light seeding kind is cultivated the strong seedling of band tiller;
According to male parent loose powder characteristics, determine row ratio rationally, be generally 1: 6;
The science water and fertilizer management makes up the high yield structure;
C, florescence prediction
The leaf age follow-up investigation is carried out in the reasonable reconnaissance in field, and investigation in every 5-7 days once;
In time gather investigation result, determine that the leaf age of each investigation is poor;
With reference to leaf age investigation records over the years, comparison leaf age developmental process finds differences and in time adjusts;
Enter the ear differentiation after date, carried out an ear differentiation process and investigate in every 2--3 days;
With reference to ear differentiation process investigation records over the years, compare young fringe developmental process, find differences and in time adjust;
D, florescence are regulated
(1), fertilizer is regulated
Before three phases of ear differentiation, find differences and to use liquid manure to regulate;
To growing the inclined to one side nitrogen fertilizer application of parent early, maternal 7-10 kilogram/mu, male parent 2-3 kilogram/mu generally can be postponed out fringe 3-5 days;
To growing slow parent's foliage-spray potassium dihydrogen phosphate 2-3 time, mu is used the 150-200 gram, converts the water kilogram, generally can go out fringe in advance about 2 days;
(2), moisture is regulated
General recover system to the reaction of water than male sterile line sensitivity;
Early late female as the father, can dry field, the developmental process of control male parent;
Early the father is slow as mother, can deep irrigation, promote the developmental process of male parent;
(3), hormone regulation
After three phases of ear differentiation, find differences and to use hormone to regulate;
Under the little situation of difference, can suitably delay the operating period of Activol,, slow partially parent be used Activol 1 gram, convert 50 kilograms of foliage-sprays of water, can ear about 2 days in advance generally in the ear differentiation 6-7 phase;
As differ greatly, can spray once in per two days, spray continuously three times, can go out in advance fringe 4-5 days;
E, artificial pollination technology
Follow to the direction walking with 3 meters long bamboo bars, impel pollen to soar with the bamboo bar is round in the middle part of paternal plant gently by heavily pushing away, extensively loose, blooming to count from the later parent of blooming reaches 30%, catches up with powder once in every 3-45 minute, every day 3-4 time;
With 5 meters long bamboo bar, hold bamboo bar middle part and walk to direction along vertical row, gently impel pollen to soar with the bamboo bar by heavily pushing away male parent, extensively loose, blooming to count from the later parent of blooming reaches 30%, catches up with powder once in every 30-45 minute, every day 3-4 time;
The large tracts of land production of hybrid seeds adopts the stay cord method to pollinate, and the nylon rope of two humans finger thickness is jogged to direction along vertical row, makes rope scrape male parent, and the male parent bounce-back is kicked up pollen is high, reaches the purpose of pollination.
CNB2005101308862A 2005-12-27 2005-12-27 High-yield seeds production technique for japonica hybrid rice Expired - Fee Related CN100391331C (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101066038B (en) * 2007-05-19 2010-09-29 王玉货 Process of cross breeding rice hybrid with high yield
CN102668976A (en) * 2012-06-11 2012-09-19 广西壮族自治区农业科学院水稻研究所 High-yield propagation method for trilineage sterile line of purple-leaf hybrid rice
CN102668975A (en) * 2012-06-11 2012-09-19 广西壮族自治区农业科学院水稻研究所 Method for producing seed by using three-line hybrid rice sterile line with purple leaf marker
CN103404431A (en) * 2013-08-23 2013-11-27 云南禾朴农业科技有限公司 Method for regulating seed production flowering phase of japonica rice
CN112136686A (en) * 2020-10-10 2020-12-29 上海市农业科学院 Method for pollinating japonica hybrid rice by mechanical assistance

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1149916C (en) * 2000-12-05 2004-05-19 黄培劲 Hybrid rice breeding method

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101066038B (en) * 2007-05-19 2010-09-29 王玉货 Process of cross breeding rice hybrid with high yield
CN102668976A (en) * 2012-06-11 2012-09-19 广西壮族自治区农业科学院水稻研究所 High-yield propagation method for trilineage sterile line of purple-leaf hybrid rice
CN102668975A (en) * 2012-06-11 2012-09-19 广西壮族自治区农业科学院水稻研究所 Method for producing seed by using three-line hybrid rice sterile line with purple leaf marker
CN102668976B (en) * 2012-06-11 2016-11-16 广西壮族自治区农业科学院水稻研究所 A kind of high-yield breeding method of purple leaf hybrid rice CMS line
CN103404431A (en) * 2013-08-23 2013-11-27 云南禾朴农业科技有限公司 Method for regulating seed production flowering phase of japonica rice
CN112136686A (en) * 2020-10-10 2020-12-29 上海市农业科学院 Method for pollinating japonica hybrid rice by mechanical assistance

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Assignee: Liaoning rice hi tech product development center

Assignor: Liaoning Rice Research Institute

Contract record no.: 2011210000005

Denomination of invention: Japonica type hybridized rice high production breeding method

Granted publication date: 20080604

License type: Common License

Open date: 20060712

Record date: 20110128

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CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20080604

Termination date: 20131227