CN102668976A - High-yield propagation method for trilineage sterile line of purple-leaf hybrid rice - Google Patents

Abstract

The invention discloses a high-yield propagation method for a trilineage sterile line of purple-leaf hybrid rice. The method comprises the following steps of: (1) arranging the seeding date of male and female parents, namely Zhihong B and Zhihong A; (2) soaking, disinfecting and sprouting seeds before seeding, and seeding when white buds are exposed from the seeds or buds have a half grain length and roots have a one grain length; (3) seeding on a seedling tray of a paddy field; (4) performing water and fertilizer management, and prevention and control of plant diseases and insect pests in a rice seedling period; (5) removing impurities from the male and female parents once in the rice seedling period; (6) ensuring that the row ratio of the Zhihong B to the Zhihong A in a seed production field is 2:(8-10) and the planting density is 6*5 inches; (7) performing water and fertilizer management, and prevention and control work of plant diseases and insect pests in the seed production field well; (8) reasonably spraying 'gibberellin'; (9) doing isolation and impurity removal work well; (10) performing artificial supplementary pollination; and (11) mechanically harvesting the male parents in time. The impurity removal can be conveniently performed through the characteristics that the hybrid rice has a purple leaf marker; and high-yield sterile line seeds with the purity of 100 percent are obtained through cultivation technology measures, artificial supplementary pollination and the like.

Description

A kind of purple leaf hybrid rice three is the high-yield breeding method of male sterile line

Technical field

The invention belongs to agricultural technology field, specifically a kind of purple leaf hybrid rice three is the high-yield breeding method of male sterile line.

Background technology

Eighties of last century seventies; The series of three-series hybrid rice that the Yuan Longping proposes is promoted in China's success large tracts of land, and over past afterwards more than 30 years, hybrid rice is on cultivated area and output; All occupy the nearly half the ratio of rice in China, for the grain security of country has been made huge contribution.But hybrid rice is because of reasons such as self-fertility and weather effects, hybrid F ₁Mixing of 3-5% often arranged, and therefore cause paddy with a toll of 5-6 hundred million kg every year.Producing highly purified crossbreed is heavily one of measure of avoiding this loss.Obtain highly purified crossbreed, breeding the high male sterile line parent of purity and output is vital link, and doing a good job of it this link can not only reduce production costs, and can also ensure the hybrid seed purity of the production of hybrid seeds.

Summary of the invention

The present invention seeks to deficiency to prior art, a kind of purple leaf hybrid rice three is provided is the high-yield breeding method of male sterile line.

A kind of purple leaf hybrid rice three is the high-yield breeding method of male sterile line, may further comprise the steps:

(1) arrange father and mother properly and originally know red B and the date of seeding of knowing red A: early breed in season in Nanning in sowing mid-March, maternal than male parent early sowing about 3-5 days or female parent broadcast first phase male parent when going out 1.0 leaves again; Male parent divided for two phases broadcast, and wherein I phase male parent and II phase male parent are about 5-10 days at interval;

(2) seed is through seed soaking sterilization and vernalization before the sowing, and seed shows money or valuables one carries unintentionally or bud is about half paddy, sows about the long paddy of root;

(3) paddy field seedling dish sowing: the seedling furrow are played in smooth good back, rice seedling bed; On the seedling furrow, spread the seedling dish, the thin paddy field mud of lay one deck on the seedling dish is evenly play and is known red A or know that red B seed is on the seedling dish of having spread field mud again; Cover the fertilizer of skim again, cover the film winter protection at last;

(4) carry out the water and fertilizer management of nursery period, the extermination of disease and insect pest: executed rice seedling bed fertilizer in preceding ten days a time in rice transplanting, execute 5 kilograms of urea for every mu; Spray agricultural chemicals one time in previous week of rice transplanting;

(5) nursery period father, female parent will carry out a removal of impurities, and green plant is pulled out, and only keep the purple plant;

(6) the red B of knowledge in breeding field is 2 with the capable ratio of the red A of knowledge: 8-10, and planting density is 6 * 5 cun; Male parent is inserted the I phase and the II phase two goes totally, and I, II phase paternal plant are Chinese character pin-shaped arrangement;

(7) carry out the water and fertilizer management of breeding the field, extermination of disease and insect pest work;

(8) rationally spray " 920 ": know when red A is eared 5-10%, know red A or know that red B begins spray " 920 "; About every mu available " 920 " amount 10 grams, divides to spray in three days, second and third day male parent know red B add spray altogether 2-3 restrain;

(9) carry out and isolate and removal of impurities work: do not plant the rice varieties of earing other same period or stagger heading stage more than 12 days with interior for 200 meters in distance breeding field; Carrying out a removal of impurities heading stage, mainly is to remove the assorted strain of the greenery that mix in female parent is capable and maintenance line is known red B plant;

(10) artificial supplementary pollination: know that will in time carry out manual work when red B is bloomed catches up with powder, catch up with pollen every day three times, guarantee the maternal enough pollen that obtains;

(11) in time slice off male parent, mechanical harvesting: after knowing that red B accomplishes the pollination mission in this season, in time it is sliced off; Avoid it and know that red A strives liquid manure; Guaranteed the ventilated of field, the maturing stage adopts grain header to gather in, and before harvesting, will clear up grain header; Avoid mixing other plant seed and rice paddy seed, finally obtain purity and reach 100% male-sterile seed.

Make things convenient for impurity elimination through its characteristic, and reach the male-sterile seed of 100% high yield through acquisition purity such as cultivation technique measure, artificial supplementary pollinations with purple leaf mark.

Embodiment

Below in conjunction with specific embodiment, the present invention is elaborated.

The technical scheme that the present invention adopts is applicable to that any to have purple leaf mark hybrid rice three be the breeding of male sterile line.The present invention is that male sterile line knows that red A is an example, details embodiment of the present invention with purple leaf three.Know red A, know that red B is the male sterile line that academy of agricultural sciences, Guangxi paddy rice research institute breeds.Concrete grammar is following:

(1) arranges father and mother properly and originally know red B and the date of seeding of knowing red A.Early breed in season in sowing mid-March in Nanning, maternal know red A than male parent know red B early ear be advisable about 3 days, so female parent than male parent early sowing about 3-5 days or female parent broadcast first phase male parent when going out 1.0 leaves again; Male parent divided for two phases broadcast, and wherein I phase male parent and II phase male parent are about 5-10 days at interval.

(2) seed is through seed soaking sterilization and vernalization before the sowing, and seed shows money or valuables one carries unintentionally or bud is about half paddy, sows about the long paddy of root.

(3) paddy field seedling dish sowing.The seedling furrow are played in smooth good back, rice seedling bed, on the seedling furrow, spread the seedling dish, and the thin paddy field mud of lay one deck on the seedling dish is evenly play and known red A or know red B seed on the seedling dish of having spread field mud again, covers the fertilizer of skim again, covers the film winter protection at last.

(4) carry out the water and fertilizer management of nursery period, the extermination of disease and insect pest.Execute rice seedling bed fertilizer in preceding ten days one time in rice transplanting, execute 5 kilograms of urea for every mu.Spray agricultural chemicals one time in previous week of rice transplanting.

(5) the nursery period father and mother originally will carry out a removal of impurities, and green plant is pulled out, and only keep the purple plant.

(6) the red B of knowledge in breeding field is 2 with the capable ratio of the red A of knowledge: 8-10, planting density is 6 * 5 cun.Male parent is inserted the I phase and the II phase two goes totally, and I, II phase paternal plant are Chinese character pin-shaped arrangement.

(7) carry out the water and fertilizer management of breeding the field, extermination of disease and insect pest work.

(8) rationally spray " 920 ".Under the normal condition, when knowing red A heading 5-10%, know that red B begins spray " 920 " with knowing red A, about every mu available " 920 " amount 10 grams, divide to spray in three days, second and third day male parent know red B add spray altogether 2-3 restrain.

(9) carry out isolation and removal of impurities work.Do not plant the rice varieties of other heading same period or stagger heading stage more than 12 days with interior for 200 meters in distance breeding field; Carrying out a removal of impurities heading stage, mainly is to remove the assorted strain of the greenery that mix in female parent is capable and maintenance line is known red B plant.

(10) artificial supplementary pollination.Know that will in time carry out manual work when red B is bloomed catches up with powder, catch up with pollen every day three times, guarantee the maternal enough pollen that obtains.

(11) in time slice off male parent, mechanical harvesting.After knowing that red B accomplishes the pollination mission in this season, in time it is sliced off, avoid it and know that red A strives liquid manure, guaranteed the ventilated of field.Maturing stage adopts grain header to gather in, and before harvesting, will clear up grain header, avoids mixing other plant seed and rice paddy seed, finally obtains purity and reaches 100% male-sterile seed.

Should be understood that, concerning those of ordinary skills, can improve or conversion, and all these improvement and conversion all should belong to the protection domain of accompanying claims of the present invention according to above-mentioned explanation.

Claims (1)

1. a purple leaf hybrid rice three is the high-yield breeding method of male sterile line, it is characterized in that, may further comprise the steps:

(1) arrange father and mother properly and originally know red B and the date of seeding of knowing red A: early breed in season in Nanning in sowing mid-March, maternal than male parent early sowing about 3-5 days or female parent broadcast first phase male parent when going out 1.0 leaves again; Male parent divided for two phases broadcast, and wherein I phase male parent and II phase male parent are about 5-10 days at interval;

(2) seed is through seed soaking sterilization and vernalization before the sowing, and seed shows money or valuables one carries unintentionally or bud is about half paddy, sows about the long paddy of root;

(3) paddy field seedling dish sowing: the seedling furrow are played in smooth good back, rice seedling bed; On the seedling furrow, spread the seedling dish, the thin paddy field mud of lay one deck on the seedling dish is evenly play and is known red A or know that red B seed is on the seedling dish of having spread field mud again; Cover the fertilizer of skim again, cover the film winter protection at last;

(4) carry out the water and fertilizer management of nursery period, the extermination of disease and insect pest: executed rice seedling bed fertilizer in preceding ten days a time in rice transplanting, execute 5 kilograms of urea for every mu; Spray agricultural chemicals one time in previous week of rice transplanting;

(5) nursery period father, female parent will carry out a removal of impurities, and green plant is pulled out, and only keep the purple plant;

(6) the red B of knowledge in breeding field is 2 with the capable ratio of the red A of knowledge: 8-10, and planting density is 6 * 5 cun; Male parent is inserted the I phase and the II phase two goes totally, and I, II phase paternal plant are Chinese character pin-shaped arrangement;

(7) carry out the water and fertilizer management of breeding the field, extermination of disease and insect pest work;

(8) rationally spray " 920 ": know when red A is eared 5-10%, know red A or know that red B begins spray " 920 "; About every mu available " 920 " amount 10 grams, divides to spray in three days, second and third day male parent know red B add spray altogether 2-3 restrain;

(9) carry out and isolate and removal of impurities work: do not plant the rice varieties of earing other same period or stagger heading stage more than 12 days with interior for 200 meters in distance breeding field; Carrying out a removal of impurities heading stage, mainly is to remove the assorted strain of the greenery that mix in female parent is capable and maintenance line is known red B plant;

(10) artificial supplementary pollination: know that will in time carry out manual work when red B is bloomed catches up with powder, catch up with pollen every day three times, guarantee the maternal enough pollen that obtains;

(11) in time slice off male parent, mechanical harvesting: after knowing that red B accomplishes the pollination mission in this season, in time it is sliced off; Avoid it and know that red A strives liquid manure; Guaranteed the ventilated of field, the maturing stage adopts grain header to gather in, and before harvesting, will clear up grain header; Avoid mixing other plant seed and rice paddy seed, finally obtain purity and reach 100% male-sterile seed.