CN110663307B - Artificial germination accelerating method for copperleaf herb seeds - Google Patents
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- 230000035784 germination Effects 0.000 title claims abstract description 51
- 241001060350 Acalypha Species 0.000 title claims abstract description 26
- 238000000034 method Methods 0.000 title claims abstract description 20
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims abstract description 52
- 229910052802 copper Inorganic materials 0.000 claims abstract description 46
- 239000010949 copper Substances 0.000 claims abstract description 46
- 210000005069 ears Anatomy 0.000 claims abstract description 30
- 241000196324 Embryophyta Species 0.000 claims abstract description 17
- 241000694589 Gesneria viridiflora Species 0.000 claims abstract description 17
- 238000005286 illumination Methods 0.000 claims description 25
- 239000003795 chemical substances by application Substances 0.000 claims description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
- 239000008213 purified water Substances 0.000 claims description 14
- 229910000365 copper sulfate Inorganic materials 0.000 claims description 11
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims description 11
- 238000005507 spraying Methods 0.000 claims description 10
- 238000012258 culturing Methods 0.000 claims description 7
- 238000003892 spreading Methods 0.000 claims description 6
- 230000003203 everyday effect Effects 0.000 claims description 5
- 238000009736 wetting Methods 0.000 claims description 5
- 241000607479 Yersinia pestis Species 0.000 claims description 4
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 claims 4
- 239000003617 indole-3-acetic acid Substances 0.000 claims 2
- 238000003306 harvesting Methods 0.000 abstract description 5
- 230000007226 seed germination Effects 0.000 abstract description 3
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 2
- 229940079593 drug Drugs 0.000 abstract description 2
- 239000003814 drug Substances 0.000 abstract description 2
- 230000006698 induction Effects 0.000 abstract description 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 9
- 241000311345 Elsholtzia haichowensis Species 0.000 description 7
- 241001549572 Elsholtzia splendens Species 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 238000010790 dilution Methods 0.000 description 4
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- 238000009826 distribution Methods 0.000 description 3
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- 238000003756 stirring Methods 0.000 description 3
- 241000131458 Elsholtzia Species 0.000 description 2
- 241000167774 Lithospermum viride Species 0.000 description 2
- 238000003723 Smelting Methods 0.000 description 2
- 230000001133 acceleration Effects 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000004898 kneading Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 1
- 235000017491 Bambusa tulda Nutrition 0.000 description 1
- 244000308746 Cucumis metuliferus Species 0.000 description 1
- 235000013554 Cucumis metuliferus Nutrition 0.000 description 1
- 241000723198 Cupressus Species 0.000 description 1
- 244000301850 Cupressus sempervirens Species 0.000 description 1
- 241000207923 Lamiaceae Species 0.000 description 1
- 241000717679 Mosla Species 0.000 description 1
- 244000082204 Phyllostachys viridis Species 0.000 description 1
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 1
- 241000209504 Poaceae Species 0.000 description 1
- 239000011425 bamboo Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229910001779 copper mineral Inorganic materials 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012549 training Methods 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
- A01N59/20—Copper
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Zoology (AREA)
- Agronomy & Crop Science (AREA)
- Soil Sciences (AREA)
- Physiology (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention belongs to the technical field of garden biology, and particularly relates to an artificial germination accelerating method for copperleaf herb seeds. The method selects the copper plant flower ears close to maturity to harvest seeds, can avoid seed loss caused by ejection when the seed ears are mature, and solves the problem of difficult collection of the copper plant flower seeds; the method adopts indoor artificial environment treatment to promote the after-ripening of the copper grass flowers and the seeds, so that the maturity of the seeds is ensured; meanwhile, the self-made germinator is adopted to induce the cupressure flower seeds to germinate, so that the problem of low seed germination rate under natural conditions is solved, and the germination rate of the cupressure flower is close to 98%; in the invention, no matter the post-ripening treatment of the copper grass flower ears or the induction of seed germination, no special facilities are needed, and the used medicines are all conventional reagents, are cheap and easy to obtain, and have low cost.
Description
Technical Field
The invention belongs to the technical field of garden biology, and particularly relates to an artificial germination accelerating method for copperleaf herb seeds.
Background
The copperleaf herb flower is a copper-resistant genotype of Elsholtzia splendens Nakai (Elsholtzia splendens Nakai) belonging to the family of labiatae and the genus of Elsholtzia, is a special plant capable of relatively accurately displaying copper mineral deposits, is called as copperleaf herb and has copper ore in places with the copperleaf herb flower. In ancient times, people searched for copper ores according to the distribution of copper grass flowers, and the copper grass flowers were used as a repairing material for copper-polluted soil in modern environmental management. The non-copper-resistant genotype of Elsholtzia splendens mainly grows in non-copper environments such as hillsides, roadside or grasses. The copper tolerance of the non-copper-tolerant genotype Elsholtzia splendens on copper is poor, after 2 generations of continuous copper stress, the surface area, the perimeter, the length and the width of the seeds, the vitality of the seeds, the quality of the hypocotyl, the fresh quality of radicle and the like of the seeds are obviously lower than the seeds generated by the plants which are not subjected to stress treatment in a control group (Lianghuijia, and the like, 2018, the influence of the continuous 2-generation copper stress treatment on the morphological size germination and the seedling growth of the seeds of the Elsholtzia splendens, Jiangsu agricultural science, 2: 209-212). Even if the copper-resistant gene type elsholtzia haichowensis produced in different mining areas has obvious difference on the reaction of copper stress, under the condition of water culture, the low-concentration copper treatment (<50 mu mol/L) has certain promotion effect on the growth of the roots of the elsholtzia haichowensis produced in Zhejiang copper mine, and when the copper concentration is more than 100 mu mol/L, the growth of the roots is obviously inhibited (Tianshenke, 2005, the influence of copper stress on the root forms of the elsholtzia haichowensis and Chinese mosla and the copper enrichment, the academy of conservation of water and soil, 3: 97-100); and in the case of the elsholtzia haichowensis grown in the copper-smelting greengromwell of Hubei province, under the condition that the copper content of the soil in the mining area is 1645-8950 mg/kg, the distribution of the elsholtzia haichowensis is obviously related to the copper content of the soil, and the distribution of the elsholtzia haichowensis is also more in the place with high copper content (Kewenshan et al, 2001, geochemical characteristics analysis and ecology report of the elsholtzia haichowensis plant in the mining area of the copper-smelting greengromwell, 6:908 and 912). This indicates that the copper tolerance of copper-resistant genotype haizhou elsholtzia is different due to different production areas, i.e. different copper-resistant ecotypes exist.
At present, the copperleaf flowers are mainly propagated through seeds, but the propagation effect is poor, and the main problems are that the germination rate of the copperleaf flowers is low under natural conditions, the manual seed collection time is difficult to grasp, the seeds are not mature early and influence the germination rate, the seeds are ejected to the ground late and are extremely fine, the seeds scattered on the ground cannot be picked up at all, and as a result, on one hand, the harvesting difficulty of the seeds is increased, on the other hand, the germination rate of the harvested seeds is greatly reduced, the large-scale propagation of the copperleaf flowers is directly influenced, and the seed collection time and the manual germination acceleration are necessarily controlled to improve the germination rate of the seeds. So far, no invention disclosure or literature report of relevant aspects exists.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide an artificial germination accelerating method for copperleaf herb seeds.
In order to achieve the purpose of the invention, the technical scheme adopted by the invention is as follows:
an artificial germination accelerating method for copperleaf herb seeds comprises the following steps:
(1) selecting a robust copper plant without pest damage as a seed collecting stock plant;
(2) respectively taking the copper grass flower seed ears close to maturity or the mature copper grass flower seed ears from the stock plants, wherein the copper grass flower seed ears close to maturity need to be placed in an indoor artificial environment for after-ripening treatment;
(3) respectively rubbing the copper grass flower seed ears which are close to maturity and are subjected to after-ripening treatment or the matured copper grass flower seed ears repeatedly until the seeds completely fall off, and collecting the seeds for later use;
(4) spreading the seeds in a culture dish with clean double-layer filter paper, enabling the seeds to be distributed uniformly as much as possible, spraying a wine germination accelerating agent into the culture dish to fully wet the filter paper, then placing the culture dish in a dark room at 25 +/-2 ℃ for continuous culture for 3-5 days, spraying the germination accelerating agent once every day according to the wetting condition of the filter paper during the period, keeping the filter paper moist, after the seeds break the chest, transferring the seeds to an illumination condition for culture for 2-3 days until the coppergrass flower seeds germinate, and obtaining the coppergrass flower bud seedlings.
In the above scheme, the treatment conditions of the after-ripening treatment are as follows: the temperature is 25 +/-2 ℃, the humidity is 40-50%, the illumination time is 9-10 hours/day and night, the illumination intensity is 2500-3000 lux, and the continuous after-ripening treatment is carried out for 20-25 days.
In the scheme, the germination accelerating agent is prepared by mixing IAA (inddo acetic acid), copper sulfate and purified water, and the germination accelerating agent comprises the following components in parts by weight: 0.05-0.055 part of indoacetic acid, 0.50-0.55 part of copper sulfate and 99.45-99.395 parts of purified water. The preparation method of the germination accelerating agent comprises the following steps: taking 1L of the germination accelerating agent as an example, 0.5mg of IAA is fully dissolved by a small amount of 95% alcohol, 600ml of purified water is added for dilution, 5.0mg of copper sulfate is added, the mixture is fully dissolved and stirred, and the purified water is added to reach the constant volume of 1L.
In the above scheme, the process of culturing under illumination conditions in step (4) comprises: the temperature is 25 +/-2 ℃, the humidity is 70-75%, the illumination time is 14-16 hours/day and night, and the illumination intensity is 2500-3000 lux.
The invention has the beneficial effects that: according to the method, the seeds can be harvested by selecting the copper grass flower ears close to maturity, the seed loss caused by ejection when the seeds are mature can be avoided, the problem that the copper grass flower seeds are difficult to collect is solved, the copper grass flower ears are promoted to be postripened by further adopting indoor artificial environment treatment, the maturity of the seeds is ensured, and the method has the advantage of high harvesting rate; meanwhile, the invention adopts the self-made germinator to induce the copperleaf flower seeds to germinate, thus solving the problem of low seed germination rate under natural conditions, and the germination rate of the copperleaf flower seeds is close to 98 percent; the whole operation process of the invention only needs simple training, and a practitioner can master the operation requirement, thus the invention is suitable for large-scale production of professional cooperative society and is also suitable for scattered planting of farmers; no matter the post-ripening treatment of the copper grass flower ears or the induction of the seeds for sprouting is carried out, no special facilities are needed, and the used medicines are all conventional reagents, are low in price, easy to obtain and low in cost.
Drawings
FIG. 1 shows the seeds of Elsholtzia cupana.
FIG. 2 shows the flower bud of Cucumis metuliferus.
Detailed Description
In order to better understand the present invention, the following examples are further provided to illustrate the present invention, but the present invention is not limited to the following examples.
Examples 1 to 3
In 2016 to 2018, every year in the last 10 th and the last 11 th of a month, selecting a sunny day, respectively collecting nearly mature seed spikes and mature seed spikes (CK) of coppergrass flowers in copper-recorded-mountain copper mine areas of Dayu city of Hubei province, randomly sampling, respectively collecting 100 plants, cutting off the seed spikes, counting, respectively spreading on bamboo dustpans, and carrying out after-ripening treatment on the nearly mature seed spikes under the environmental conditions provided by the invention: the temperature was 27 ℃, the humidity was 50%, the number of hours of light irradiation was 10 hours/day and the light irradiation intensity was 3000lux, and the after-ripening treatment was continued for 25 days. After the after-ripening treatment is finished, respectively rubbing the poor seed ears, and respectively counting and comparing the quantity of the copperleaf flower seeds obtained by the seed ears close to the mature seed ears and the mature seed ears by adopting the after-ripening treatment of the invention. The results show that 85.33 seeds are obtained by post-ripening immature spike flat seeds and 42 seeds are obtained by mature spike (CK) spike flat seeds (the results are shown in Table 1), and the difference between the seeds is obvious.
TABLE 1 comparison of the ratio of the earnings of the panicles close to maturity and the seeds of the panicles already matured by the after-ripening treatment of the present invention for 3 consecutive years
Note: collecting 100 strains of immature seed ears and mature seed ears of the copperleaf flowers every year.
In the winter from 2016 to 2018, by adopting the technical measures provided by the invention, indoor artificial germination tests are respectively carried out on the seeds collected in the embodiments 1-3, 200 seeds are taken for each treatment, and the three times of the experiments are repeated. The artificial germination accelerating method comprises the following steps: the method comprises the following steps of spreading seeds in a culture dish with clean double-layer filter paper, enabling the seeds to be distributed uniformly as much as possible, spraying a wine germination accelerating agent into the culture dish, enabling the filter paper to be fully wet, then placing the culture dish in a dark room at 25 +/-2 ℃ for 3-5 days, spraying the germination accelerating agent once every day according to the wetting condition of the filter paper during the period, keeping the filter paper wet, after the seeds break the chest, transferring the seeds to an illumination condition for culturing for 2-3 days, and then germinating the seeds of the copperleaf flowers, wherein the germination accelerating agent is formed by mixing IAA (indoacetic acid), copper sulfate and purified water, and the preparation method comprises the following steps: preparing 1000ml of germinator, dissolving 0.5mg IAA with a small amount of 95% alcohol, adding 600ml of purified water for dilution, then adding 5.0mg copper sulfate, dissolving and stirring completely, and adding purified water to reach the constant volume of 1000 ml; the culture illumination conditions are as follows: the temperature is 27 ℃, the humidity is 75%, the illumination time is 16 hours/day and night, and the illumination intensity is 3000 lux.
Seeds collected from nearly mature spikes of cupressure flowers without after-ripening were used as Controls (CK). Germination rates were calculated separately and the differences between each treatment and the control were compared. The results show that the average germination rates of the cupressus flowers treated by the germination accelerating method, which are close to the average germination rates of the seeds collected by the mature ears and the average germination rates of the seeds collected by the mature ears are 97.83 percent and 97.78 percent respectively, and the two germination rates are not obviously different; the germination rate of seeds collected from the ears of cupressus sempervirens which were not treated by the germination accelerating method of the present invention and were close to maturity was 54.22% (see table 2 for results).
TABLE 2 comparison of germination rates of seeds collected from nearly mature ears of cuprum flowers treated by germination acceleration according to the present invention and seeds collected from mature ears of cuprum flowers for 3 consecutive years
Note: three replicates of 200 seeds were used.
Example 4
An artificial germination accelerating method for copperleaf herb seeds comprises the following steps:
(1) selecting a robust copper plant without pest damage as a seed collecting stock plant;
(2) respectively taking off the nearly mature cuprum flower seed ears from the stock plant by using scissors, and placing the nearly mature cuprum flower seed ears in an indoor artificial environment for after-ripening treatment: the temperature is 23 ℃, the humidity is 40%, the illumination time is 9 hours/day and night, the illumination intensity is 2500lux, and the continuous after-ripening treatment is carried out for 20 days;
(3) repeatedly kneading the copper grass flower seed ears which are subjected to after-ripening treatment and are close to ripeness until the seeds completely fall off, and collecting the seeds for later use;
(4) spreading the seeds in a culture dish with clean double-layer filter paper, uniformly distributing the seeds as much as possible, spraying a wine germination accelerating agent into the culture dish to fully wet the filter paper, then placing the culture dish in a dark room at 25 +/-2 ℃ for continuous culture for 3-5 days, spraying the germination accelerating agent once every day according to the wetting condition of the filter paper during the continuous culture, keeping the filter paper moist, and after the seeds break the chest, transferring the culture dish to a lighting condition for culture for 2-3 days, and germinating the coppergrass seeds; the germination accelerating agent is prepared by mixing IAA (inddol acetic acid), copper sulfate and purified water, taking 1000ml of germination accelerating agent as an example, 0.55mg of IAA is fully dissolved by a small amount of 95% alcohol, 600ml of purified water is added for dilution, 5.5mg of copper sulfate is added, and after full dissolution and stirring, the purified water is added to reach the volume of 1000 ml. The illumination conditions are as follows: the temperature is 23 deg.C, the humidity is 70%, the illumination time is 14 hr/day and night, the illumination intensity is 2500lux, and the after-ripening treatment is continued for 20 days.
In the present example, the harvest rate of the copperleaf flower seeds is 98.5%, and the germination rate of the seeds is 97.5%.
Example 5
An artificial germination accelerating method for copperleaf herb seeds comprises the following steps:
(1) selecting a robust copper plant without pest damage as a seed collecting stock plant;
(2) respectively taking off the nearly mature cuprum flower seed ears from the stock plant by using scissors, and placing the nearly mature cuprum flower seed ears in an indoor artificial environment for after-ripening treatment: continuously performing after-ripening treatment at 25 deg.C, humidity of 45%, illumination time of 9.5 hr/day and illumination intensity of 2700lux for 23 days;
(3) repeatedly kneading the copper grass flower seed ears which are subjected to after-ripening treatment and are close to ripeness until the seeds completely fall off, and collecting the seeds for later use;
(4) spreading seeds in a culture dish with clean double-layer filter paper, uniformly distributing the seeds as much as possible, spraying a wine germination accelerating agent into the culture dish to fully wet the filter paper, continuously culturing for 3-5 days in a dark room at 25 ℃, spraying the germination accelerating agent once every day according to the wetting condition of the filter paper during the period, keeping the filter paper wet, and after the seeds break the chest, transferring to the illumination condition for culturing for 2-3 days, and germinating the copperleaf seeds; the germination accelerating agent is prepared by mixing IAA (inddol acetic acid), copper sulfate and purified water, taking 1000ml of germination accelerating agent as an example, 0.53mg of IAA is fully dissolved by a small amount of 95% alcohol, then the purified water is added for dilution, then 5.3mg of copper sulfate is added, the pure water is added for constant volume to 1000ml after the full dissolution and stirring. The illumination conditions are as follows: the temperature was 25 ℃, the humidity was 73%, the number of hours of light irradiation was 15 hours/day and the light intensity was 2700lux, and the after-ripening treatment was continued for 23 days.
In this example, the harvest rate of the copperleaf flower seeds is 99.5%, and the germination rate of the seeds is 97%.
It is apparent that the above embodiments are only examples for clearly illustrating and do not limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications are therefore intended to be included within the scope of the invention as claimed.
Claims (1)
1. An artificial germination accelerating method for copperleaf herb seeds is characterized by comprising the following steps:
(1) selecting a robust copper plant without pest damage as a seed collecting stock plant;
(2) respectively taking the nearly mature copperleaf flower seed spikes or the mature copperleaf flower seed spikes from the stock plant, wherein the nearly mature copperleaf flower seed spikes need to be placed in an indoor artificial environment for after-ripening treatment;
the treatment conditions of the after-ripening treatment are as follows: the temperature is 25 +/-2 ℃, the humidity is 40-50%, the illumination time is 9-10 hours/day and night, the illumination intensity is 2500-3000 lux, and the continuous after-ripening treatment is carried out for 20-25 days;
(3) respectively rubbing the copper grass flower seed ears which are close to maturity and are subjected to after-ripening treatment or the matured copper grass flower seed ears repeatedly until the seeds completely fall off, and collecting the seeds for later use;
(4) spreading seeds in a culture dish with clean double-layer filter paper, uniformly distributing the seeds as much as possible, spraying a germination accelerating agent into the culture dish to fully wet the filter paper, continuously culturing for 3-5 days in a dark room at 25 +/-2 ℃, spraying the germination accelerating agent once every day according to the wetting condition of the filter paper during the period, keeping the filter paper wet, transferring the seeds to the illumination condition after the seeds break the chest, culturing for 2-3 days until the coppergrass flower seeds germinate, and obtaining coppergrass flower bud seedlings;
the germination accelerating agent is prepared by mixing indoleacetic acid, copper sulfate and purified water, and comprises the following components in parts by weight: 0.05-0.055 part of indoleacetic acid, 0.50-0.55 part of copper sulfate and 99.45-99.395 parts of purified water;
the process for culturing under the illumination condition comprises the following steps: the temperature is 25 +/-2 ℃, the humidity is 70-75%, the illumination time is 14-16 hours/day and night, and the illumination intensity is 2500-3000 lux.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105665430A (en) * | 2016-02-03 | 2016-06-15 | 常州市鼎日环保科技有限公司 | Cultivation method for copper weeds for treating copper contaminated soil |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105665430A (en) * | 2016-02-03 | 2016-06-15 | 常州市鼎日环保科技有限公司 | Cultivation method for copper weeds for treating copper contaminated soil |
Non-Patent Citations (3)
| Title |
|---|
| Apigenin-7-O-β-D-glycoside isolation from the highly copper-tolerant plant Elsholtzia splendens;Hong-yun PENG et al;《Biomed & Biotechnol》;20161231;第17卷(第6期);第447-454页 * |
| 紫花香薷种子萌发特性试验;刘月等;《湖北民族学院学报(自然科学版)》;20100930;第28卷(第03期);第302-304，第307页"第302页第2段，第303-304页第1.1-2.2节" * |
| 连续2代铜胁迫处理对海州香薷种子形态大小、萌发及幼苗生长的影响;梁慧娟等;《江苏农业科学》;20180122;第46卷(第02期);第209-212页"第209-210页第1.3-2.2节及图1-2，第211页第3节" * |
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