CN101755683A - Applications of Elytrigia elongata to genetic improvement of wheat - Google Patents
Applications of Elytrigia elongata to genetic improvement of wheat Download PDFInfo
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- CN101755683A CN101755683A CN200810238644A CN200810238644A CN101755683A CN 101755683 A CN101755683 A CN 101755683A CN 200810238644 A CN200810238644 A CN 200810238644A CN 200810238644 A CN200810238644 A CN 200810238644A CN 101755683 A CN101755683 A CN 101755683A
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- couchgrass
- fringe couchgrass
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/13—Abiotic stress
- Y02A40/132—Plants tolerant to drought
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/13—Abiotic stress
- Y02A40/135—Plants tolerant to salinity
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention relates to applications of Elytrigia elongata to the genetic improvement of wheat. The Elytrigia elongata (Elytrigia elongata(Host)Nevski=Agropyron elongatum(Host)Beauv.=Thinopyrum elongatum(Host)D.R.Dewey=Thinopyrum ponticum Podp.2n=70,StStStStEeEeEbEbExEx) is a gramineae quackgrass plant, is originated in southeastern Europe and Asia Minor and is mainly distributed in Mediterranean coast and the inland of the Middle East and the former Soviet Union. The breed has a plurality of excellent properties, such as luxuriant growth with a plurality of flowers and fruits, resistance of a plurality of wheat diseases (three rust diseases, powdery mildew disease, yellow dwarf disease, and the like), resistance of coldness, saline, alkaline, drought, moisture and leanness, high protein, and the like, thereby being a rare excellent foreign gene supplier for improving the wheat properties.
Description
Technical field
The application of long fringe couchgrass in genetic improvement of wheat belongs to the Crop Genetics and Breeding field.
Background technology
Long fringe couchgrass is a grass family wheat family couchgrass platymiscium, originates in the European southeast and Asia Minor, mainly is distributed in the hinterland of the Mediterranean bank and the Middle East and the former Soviet Union.These species have many good proterties, as grow prolifically, spend more many real, anti-multiple wheat diseases (three rust, white powder, yellow dwarf etc.), cold resistance, salt tolerant alkali, drought-resistant, than moisture-proof, impoverishment tolerant and high protein etc., be the rare excellent foreign gene donor of wheat character improvement.
Summary of the invention
Long fringe couchgrass is to one of bigger foreign gene donor kind of wheat breeding contribution, Li Zhensheng etc. with common wheat and long fringe couchgrass selection cross gone out high yield, disease-resistant New Winter Wheat Variety is laid down No. 6 for a short time.For a short time laying down No. 6 is that China uses desirable genes that the distant hybridization technology successfully will long fringe couchgrass to import wheat at first and obtains the wheat distance edge hybrid new varieties of important breakthrough, and popularizing area was 1,000 ten thousand mu in the highest year.Subsequently, they again with lay down for a short time No. 6 be basic material, selected that good, the degeneration-resistant and disease resistance of flower characters is strong, the new variety of wheat of wide adaptability, stable yield lays down 54 for a short time.Photosensitive grade of summer utilizes the chromosome small fragment that cell-fusion techniques will long fringe couchgrass to import Jinan 177, obtains hybrid strain, again through systematic breeding obtain disease-resistant, good salt tolerance, melt No. 3 on new variety of wheat mountain that protein content is high.
The filial generation of long fringe couchgrass and common wheat, though have can not have some prominent features characteristic as the kind utilization, can be as the parent material utilization in wheat breeding.Octoploid of wheat-wheatgrass is the new species that utilizes long fringe couchgrass and common wheat hybridization to create, and comprises a complete set of chromosome of common wheat and one group of couchgrass chromosome, has many merits of couchgrass.Therefore, utilizing octoploid of wheat-wheatgrass to make cross materials New Wheat Germplasm Created, seed selection for new variety wheat, is the effective way of carrying out genetic improvement of wheat.It 759 be that the parent is hybridized for No. 1 with high yield that the alien addition line (2n=44) that produces with common wheat and the hybridization of long fringe couchgrass is laid down for a short time, having selected improved seeds such as lay down for a short time No. 4.The overseas utilization multiple cross has the chromosome substitution of Lr19 gene with common wheat 7D chromosome and long fringe couchgrass, does maternally with this substitution line, has successfully selected the sick new lines of rust-proofing.The breeding work person has obtained alien addition line, alien substitution and translocation line (Zhang Xueyong, 1989 of a collection of wheat-long fringe couchgrass through long-term effort both at home and abroad; Sun Shandeng, 1990; Li Zhensheng, 1985; Lucky perfectly sound, 1999; Wang Honggang, 1999,2000,2003; Li Dandan, 2003; Liu, 2002; Fourth is refined, and 2003; Liu Aifeng, 2007).
Anti-bar rust gene YrTp1 and YrE (Yin Xuegui etc., 2006 in the long fringe couchgrass; Horse is gradually new etc., 1999), anti-leaf rust gene Lr19, Lr24 and Lr29 (Caldwell et al, 1956; Browder, 1980; McIntosh, 1991), anti-stem rust gene Sr24 and Sr26 (Knott, 1961; Smith et al, 1968; McIntosh, 1991) and some resistant gene of salt (Dvorak, 1985; Shannon, 1978) successfully transferred in the general wheat background, and in the genetic improvement of wheat breed, brought into play important function.Long fringe couchgrass is one of important anti-source of luteovirus (BYDV), and Chinese scholars utilizes it as anti-source, has bred intermediate materials (Sando, 1930 of a collection of Chinese People's Anti-Japanese Military and Political College wheat yellow dwarf virus; Cisar, 1992; Comeau, 1997; Li Zhensheng, 1985; Zhang, 1998).
Long fringe couchgrass genetic material for shifting in the wheat can utilize special geochemical exploration pin of long fringe couchgrass genome or multiple molecular labeling to identify.
Embodiment
Long fringe couchgrass is to one of bigger foreign gene donor kind of wheat breeding contribution, Li Zhensheng etc. with common wheat and long fringe couchgrass selection cross gone out high yield, disease-resistant New Winter Wheat Variety is laid down No. 6 for a short time.For a short time laying down No. 6 is that China uses desirable genes that the distant hybridization technology successfully will long fringe couchgrass to import wheat at first and obtains the wheat distance edge hybrid new varieties of important breakthrough, and popularizing area was 1,000 ten thousand mu in the highest year.Subsequently, they again with lay down for a short time No. 6 be basic material, selected that good, the degeneration-resistant and disease resistance of flower characters is strong, the new variety of wheat of wide adaptability, stable yield lays down 54 for a short time.Photosensitive grade of summer utilizes the chromosome small fragment that cell-fusion techniques will long fringe couchgrass to import Jinan 177, obtains hybrid strain, again through systematic breeding obtain disease-resistant, good salt tolerance, melt No. 3 on new variety of wheat mountain that protein content is high.
The filial generation of long fringe couchgrass and common wheat, though have can not have some prominent features characteristic as the kind utilization, can be as the parent material utilization in wheat breeding.Octoploid of wheat-wheatgrass is the new species that utilizes long fringe couchgrass and common wheat hybridization to create, and comprises a complete set of chromosome of common wheat and one group of couchgrass chromosome, has many merits of couchgrass.Therefore, utilizing octoploid of wheat-wheatgrass to make cross materials New Wheat Germplasm Created, seed selection for new variety wheat, is the effective way of carrying out genetic improvement of wheat.Wang Honggang (1989) etc. are intermediate materials with the octoploid of wheat-wheatgrass, with common wheat hybridization, have selected 10 elite germplasm systems, and wherein, 2 product are the monomer alien addition line, and 1 product is two monomer alien addition lines, and 2 product are the binary alien addition line.These germplasm lines have many merits of couchgrass, and comprehensive proterties is better, has high yield characteristic preferably.Zhong Guanchang etc. (1995) utilize the hybridization of octoploid of wheat-wheatgrass and common wheat, have created some alien addition lines and alien substitution, and select special precocious, of short stem, disease-resistant a, high yield, good quality wheat new varieties " morning excellent 504 ".
It 759 be that the parent is hybridized for No. 1 with high yield that the alien addition line (2n=44) that produces with common wheat and the hybridization of long fringe couchgrass is laid down for a short time, having selected improved seeds such as lay down for a short time No. 4.The overseas utilization multiple cross has the chromosome substitution of Lr19 gene with common wheat 7D chromosome and long fringe couchgrass, does maternally with this substitution line, has successfully selected the sick new lines of rust-proofing.The breeding work person has obtained alien addition line, alien substitution and the translocation line of a collection of wheat-long fringe couchgrass through long-term effort both at home and abroad.
Anti-bar rust gene YrTp1 and YrE (Yin Xuegui etc., 2006 in the long fringe couchgrass; Horse is gradually new etc., 1999), anti-leaf rust gene Lr19, Lr24 and Lr29 (Caldwell et al, 1956; Browder, 1980; McIntosh, 1991), anti-stem rust gene Sr24 and Sr26 (Knott, 1961; Smith et al, 1968; McIntosh, 1991) and some resistant gene of salt (Dvorak, 1985; Shannon, 1978) successfully transferred in the general wheat background, and in the genetic improvement of wheat breed, brought into play important function.Long fringe couchgrass is one of important anti-source of luteovirus (BYDV), and Chinese scholars utilizes it as anti-source, has bred intermediate materials (Sando, 1930 of a collection of Chinese People's Anti-Japanese Military and Political College wheat yellow dwarf virus; Cisar, 1992; Comeau, 1997; Li Zhensheng, 1985; Zhang, 1998).
Long fringe couchgrass genetic material for shifting in the wheat can utilize special geochemical exploration pin of long fringe couchgrass genome or multiple molecular labeling to identify.Appels etc. (1984) clone E genome specific repetitive sequence first from long fringe couchgrass.In 50000 bacteriophage lambda libraries, there are 4 repetitive sequences and long fringe couchgrass to have very strong hybridization signal and very weak with the hybridization signal of wheat.Wherein, the hybridization banding pattern of Ele145 in long fringe couchgrass and wheat is different.McIntyre etc. (1988) have found the repetitive sequence of a 1.4kb in λ Ele145 clone, this sequence has the characteristics of Ele145 sequence family.Zhang etc. (1990) have cloned two repetitive sequence pLeUCD1 and pLeUCD2 from the long fringe couchgrass of dliploid, length is respectively 230bp and 300bp, their content in the E genome is very abundant, and content is then very low in wheat, is the genomic special repetitive sequence of E.Bourniva etc. (1993) clone the special repetitive sequence 1-E6Hc of a long fringe couchgrass species II-1, and this sequence all has distribution on all chromosomes of E genome, but a distributed points is only arranged in wheat.This sequence has been used to seek the broken site of wheat-long fringe couchgrass translocation line.Horse is gradually newly waited (1999) discovering a cover wheat-long fringe couchgrass disome substitution line stripe rust resistance and heredity and biochemical character, long fringe couchgrass carries new anti-stripe rust of wheat gene, be positioned at 3E chromosome, in Wheat Background, be dominant inheritance, tentative YrE by name.And will be long the structural gene of fringe couchgrass coding esterase-5 be positioned 3E chromosome, called after Est-E5 temporarily.F
2Population analysis find Est-E5 and disease-resistant gene YrE be divided into from, show that Est-E5 is the detection 3E chromosome and the more satisfactory biochemical marker site of Stripe Rust Resistance Gene of carrying thereof.Wang Hong has just waited (2001,2003) utilized the RAPD technical appraisement lay down for a short time wheat translocation line and alien substitution of seed selection from long fringe couchgrass and wheat hybridizing offspring, has filtered out 2 primer OPE
13And OPH
15, can in germplasm line, stably amplify the specific DNA fragment of long fringe couchgrass.Liu Shubing etc. (1998) utilize the RAPD technology to set up the chromosomal special RAPD mark of long fringe couchgrass 1E, 3E.Liu Aifeng etc. (2007) utilize the SSR technical appraisement wheat binary alien addition line of laying down for a short time, obtain the specific molecular marker BARC165 of 1 long fringe couchgrass
268, with the repetitive sequence of this specific fragment reclaim, the clone, and be prepared into specific probe and carried out genomic in situ hybridization (GISH) and identify, can be used for following the tracks of and detect the long fringe couchgrass of decaploid genetic material in the Wheat Background.
Claims (3)
1. the filial generation of long fringe couchgrass and common wheat, though what have can not be as the kind utilization, but has some prominent features characteristic, can be as the parent material utilization in wheat breeding, octoploid of wheat-wheatgrass is the new species that utilizes long fringe couchgrass and common wheat hybridization to create, comprise a complete set of chromosome of common wheat and one group of couchgrass chromosome, have many merits of couchgrass.Therefore, utilizing octoploid of wheat-wheatgrass to make cross materials New Wheat Germplasm Created, seed selection for new variety wheat, is the effective way of carrying out genetic improvement of wheat.
2. to lay down for a short time 759 be the parent and get bumper crops No. 1 to hybridize to the alien addition line (2n=44) that produces with common wheat and the hybridization of long fringe couchgrass, having selected lays down for a short time waits improved seeds No. 4, the overseas utilization multiple cross has common wheat 7D chromosome and long fringe couchgrass the chromosome substitution of Lr19 gene, do maternal with this substitution line, successfully selected the sick new lines of rust-proofing, the breeding work person has obtained alien addition line, alien substitution and the translocation line of a collection of wheat-long fringe couchgrass through long-term effort both at home and abroad.
3. utilize the SSR technical appraisement wheat binary alien addition line of laying down for a short time, obtain the specific molecular marker BARC165 of 1 long fringe couchgrass
268, with the repetitive sequence of this specific fragment reclaim, the clone, and be prepared into specific probe and carried out genomic in situ hybridization (GISH) and identify, can be used for following the tracks of and detect the long fringe couchgrass of decaploid genetic material in the Wheat Background.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN200810238644A CN101755683A (en) | 2008-12-19 | 2008-12-19 | Applications of Elytrigia elongata to genetic improvement of wheat |
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| CN200810238644A CN101755683A (en) | 2008-12-19 | 2008-12-19 | Applications of Elytrigia elongata to genetic improvement of wheat |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101956012A (en) * | 2010-09-30 | 2011-01-26 | 河北农业大学 | Method for identifying common head cabbage-Chinese cabbage alien addition line |
| CN102334414A (en) * | 2010-07-23 | 2012-02-01 | 沈天民 | Culture method for perennial trititrigia |
| CN106416710A (en) * | 2016-10-09 | 2017-02-22 | 漯河市农业科学院 | Method for increasing distant hybridization setting rate of Elytrigia elongata and Triticum aestivum |
| CN110106280A (en) * | 2019-05-30 | 2019-08-09 | 南京农业大学 | Oligonucleotide probe kit and usage thereof that is a kind of while detecting wheat and hundred Sa couchgrass chromosomes |
-
2008
- 2008-12-19 CN CN200810238644A patent/CN101755683A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102334414A (en) * | 2010-07-23 | 2012-02-01 | 沈天民 | Culture method for perennial trititrigia |
| CN102334414B (en) * | 2010-07-23 | 2013-07-03 | 沈天民 | Culture method for perennial trititrigia |
| CN101956012A (en) * | 2010-09-30 | 2011-01-26 | 河北农业大学 | Method for identifying common head cabbage-Chinese cabbage alien addition line |
| CN101956012B (en) * | 2010-09-30 | 2012-07-04 | 河北农业大学 | Method for identifying common head cabbage-Chinese cabbage alien addition line |
| CN106416710A (en) * | 2016-10-09 | 2017-02-22 | 漯河市农业科学院 | Method for increasing distant hybridization setting rate of Elytrigia elongata and Triticum aestivum |
| CN106416710B (en) * | 2016-10-09 | 2019-11-05 | 漯河市农业科学院 | A method of improving E. elongata and common wheat Distant crossing siliqua-bearing rate |
| CN110106280A (en) * | 2019-05-30 | 2019-08-09 | 南京农业大学 | Oligonucleotide probe kit and usage thereof that is a kind of while detecting wheat and hundred Sa couchgrass chromosomes |
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Open date: 20100630 |