CN106688589A - Cotton test tube seedling trench transplanting method - Google Patents
Cotton test tube seedling trench transplanting method Download PDFInfo
- Publication number
- CN106688589A CN106688589A CN201710028477.4A CN201710028477A CN106688589A CN 106688589 A CN106688589 A CN 106688589A CN 201710028477 A CN201710028477 A CN 201710028477A CN 106688589 A CN106688589 A CN 106688589A
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- Prior art keywords
- grafting
- trench
- test tube
- cotton
- transplanting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/30—Grafting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Biodiversity & Conservation Biology (AREA)
- Forests & Forestry (AREA)
- Ecology (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Toxicology (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Materials For Medical Uses (AREA)
Abstract
The invention discloses a cotton test tube seedling trench transplanting method which includes the steps: 1) grafting cotton test tube seedlings to obtain grafting seedlings; 2) cultivating the grafting seedlings in trenches covering a shade frame to obtain the grafting seedlings cultivated in the trenches; 3) transferring the grafting seedlings cultivated in the trenches in a ground shade frame to cultivate to obtain the grafting seedlings cultivated in the shade frame; 4) transplanting the grafting seedlings cultivated in the shade frame in large land to achieve transplanting of cotton test tube seedlings. The width of each trench ranges from 50cm to 100cm, and the depth of each trench ranges from 80cm to 120cm. According to the transplanting method, experimental results show under the micro-environmental conditions formed by the trenches, grafting, transplanting and surviving of test tube seedlings are facilitated, and the test tube seedlings can still be transplanted under the conditions of high temperature, high light and air drying in summer in north by the transplanting method.
Description
Technical field
The present invention relates to biological technical field, more particularly to one grows cotton test tube seedling trench method for transplanting.
Background technology
Tissue cultures seedling out is referred to as test tube seedling or tissue-cultured seedling.Test tube seedling long term growth is in constant temperature, high humidity, dim light, nothing
It is huge with external environment difference in the closed environment of bacterium.Influenceed by this environment, cotton test tube seedling grows thin and delicate, cauline leaf table
Face cuticula is undeveloped；The photosynthesis of chloroplaset is poor；Stoma of Leaves number is few, poor activity；Root system is undeveloped, absorption function
Difference；Adaptability and resistivity to adverse circumstance is poor.
The ability that exactly internal and external environment difference is big, test tube seedling adapts to external environment, in northern dry weather, directly transplants
Cotton test tube shoot survival percent is very low.Scion (is generally done, grafting is sent out in root system with test tube seedling using the method for graft and transplantation test tube seedling
On the cotton seedling for reaching).But, summer (7,8, the 9 three months) temperature in the north is high, and illumination is extremely strong, is air-dried, even if
Graft and transplantation is also very low survival rate.For cotton, tissue culture differentiation test tube seedling will be by embryonic differentiation approach, relative to it
His plant is more difficult, and cotton test tube seedling transplanting survival rate directly affects the success or failure of whole tissue culture work.
The content of the invention
Grown cotton test tube seedling method for transplanting it is an object of the invention to provide one.
The method that the present invention is provided, comprises the following steps：
1) by cotton test tube seedling grafting, grafting is obtained；
2) grafting is cultivated in the trench for be coated with cool canopy, obtains grafting after trench culture；
3) cool canopy culture is carried out under grafting after the trench culture being moved into ground cool canopy, grafting after cool canopy culture is obtained
Seedling；
4) by grafting transplantation of seedlings crop field after the cool canopy culture, cotton test tube transplantation of seedlings is realized；
The width of the trench is 50-100cm, and depth is 80-120cm.
In the above method, the width of the trench is 80cm, and depth is 100cm.
In the above method, 25 DEG C -30 DEG C or so of temperature, humidity in the trench are 70%-90% or so, intensity of illumination
It is 1500-2200LX or so.
Or the cool canopy is rain-proof shade.
In the above method, the time of the cotton test tube method for transplanting is summer.
In the above method, the place of the cotton test tube method for transplanting is north of China, specially Shanxi, and embodiment exists
Yuncheng, Shanxi is carried out.
Yuncheng, Shanxi summer mean daily temperature is 30-40 DEG C or so, per day intensity of illumination is 3000-4000LX or so,
Per day air humidity is 30%-70% or so.
In the above method, step 2) in, the grafting is cultivated in trench includes following A and B：
A, the grafting cover film is placed in trench and is cultivated to the grafting wound healing, removed described thin
Film, obtains removing the grafting of film；
It is above-mentioned be placed in culture in trench and be specially to be placed in after trench to the grafting wound healing start within the 7th day discharge hole and lead to
Gas, it is ascending, until interface substantially expands wound healing.
B, by it is described removal film grafting continue trench place cultivate, obtain trench transplant after grafting.
In the above method, in step A, the incubation time is 20-30 days；
In step B, the incubation time is 3-5 days.
In the above method, in step 3) in, the cool canopy incubation time is 1-2 weeks；
Moved under sunlight daily during the mode of the cool canopy culture for several times, time first short and then long, typically from the morning 10 when
During to afternoon 4, remaining time is in cool canopy.
Another object of the present invention is to provide a kind of cotton grafting seedling trench method for transplanting.
The method that the present invention is provided, comprises the following steps：Step 2 in the above method) -4).
In the above method, the cotton test tube seedling is No. 35 test tube seedlings of middle cotton or other kind test tube seedlings；
The stock that the grafting is used is sea island cotton seedling or the strong cotton variety of other well developed root systems, resistance of reverse.
Environmental condition directly affects the effect of test tube seedling graft and transplantation, it is important to light, temperature during controlling grafting, wet.
The present invention is it is demonstrated experimentally that the subenvironment condition that is formed of trench, meets the dim light required by test tube seedling grafting
(1500-2200LX), suitable constant temperature (25 DEG C -30 DEG C), suitable humidity (70%-90%), are conducive to test tube seedling grafting to move
Cultivation is survived.Use this transplantation technique, in northern summer high temperature, high light, be air-dried under conditions of remain able to carry out test tube seedling shifting
Plant.
Specific embodiment
Experimental technique used in following embodiments is conventional method unless otherwise specified.
Material used, reagent etc. in following embodiments, unless otherwise specified, commercially obtain.
Embodiment 1, cotton test tube seedling trench method for transplanting
1st, the preparation of trench and graft
1) trench is excavated
Yuncheng, Shanxi July summer dug under the shade of Cotton Research Institute, Shanxi Academy of Agricultural Sciences solarium one it is long:
200cm or so, width:80cm, depth:The trench (under the cool canopy of common rain-proof) of 100cm, 25 DEG C or so of the temperature in trench, ground
Humidity in ditch is 80% or so, and the intensity of illumination in trench is 1500LX or so.
2), graft
Choosing the length in flowerpot has the sea island cotton seedling of 3-4 piece true leaves as stock, with No. 35 tissue cultures of cotton in 50 plants
(hypocotyl of 35 cotton aseptic seedlings is that explant carries out tissue cultures to regeneration cotton test tube seedling in, by embryonic differentiation approach
The cotton regenerated plant for obtaining) scion is done respectively carries out grafting, obtain grafting in the cotton test tube seedling of flowerpot, as grafting.
2nd, trench is transplanted
It is positioned in 1 trench for obtaining after the cotton test tube seedling covering thin transparent film for grafting in flowerpot that above-mentioned 1 is obtained,
Carry out following trench and transplant culture：
First the 7th day after trench is placed in starts discharge hole ventilation, ascending, until interface substantially expands, (explanation otch is
Through healing, survive substantially), remove interface cotton rope and remove transparent membrane completely, obtain within about 20 days removing grafting in for film
The cotton test tube seedling of flowerpot；
The cotton test tube seedling for grafting in flowerpot that film will be removed again continues to place culture 3 days in trench, obtains trench shifting
Grafting after cultivation.
3rd, cool canopy culture
(period moves to number under sunlight daily within one week for culture under the test tube seedling of grafting survival in trench to be moved on to the cool canopy on ground
It is secondary, time first short and then long, typically from the morning 10 up to afternoon 4 when), obtain grafting after cool canopy culture.
4th, crop field is moved into
Grafting after cool canopy culture is moved into crop field.
50 plants of experiment grafting test tube seedling, survives 35 plants, survival rate 70%.
Embodiment 2, cotton test tube seedling trench method for transplanting
Method with embodiment 1 is essentially identical, and different is only by No. 35 tissue cultures regeneration cotton test tubes of cotton in 50 plants
Seedling replaces with No. 3 tissue cultures regeneration cotton test tube seedlings of 30 plants of nasal mucus cottons.
As a result, 30 plants of experiment grafting test tube seedling, survives 18 plants, survival rate 60%.
Claims (10)
1. one grow cotton test tube seedling method for transplanting, comprise the following steps：
1) by cotton test tube seedling grafting, grafting is obtained；
2) grafting is cultivated in the trench for be coated with cool canopy, obtains grafting after trench culture；
3) cool canopy culture is carried out under grafting after the trench culture being moved into ground cool canopy, grafting after cool canopy culture is obtained；
4) by grafting transplantation of seedlings crop field after the cool canopy culture, cotton test tube transplantation of seedlings is realized；
The width of the trench is 50-100cm, and depth is 80-120cm.
2. method according to claim 1, it is characterised in that：The width of the trench is 80cm, and depth is 100cm.
3. method according to claim 1 and 2, it is characterised in that：
25 DEG C -30 DEG C of temperature, humidity in the trench are 70%-90%, intensity of illumination is 1500-2200LX；
Or, the cool canopy is rain-proof shade.
4. according to any described method in claim 1-3, it is characterised in that：The time of the cotton test tube method for transplanting
It is summer.
5. according to any described method in claim 1-4, it is characterised in that：The place of the cotton test tube method for transplanting
It is north of China.
6. according to any described method in claim 1-5, it is characterised in that：Step 2) in, the grafting is in trench
Culture includes following A and B：
A, the grafting cover film is placed in trench and is cultivated to the grafting wound healing, remove the film, obtained
To the grafting of removal film；
B, by it is described removal film grafting continue trench place cultivate, obtain trench transplant after grafting.
7. method according to claim 6, it is characterised in that：
In step A, the incubation time is 20-30 days；
In step B, the incubation time is 3-5 days.
8. according to any described method in claim 1-7, it is characterised in that：
In step 3) in, the cool canopy incubation time is 1-2 weeks.
9. a kind of cotton grafting seedling trench method for transplanting, comprises the following steps：Step in claim 1-8 in any methods described
Rapid 2) -4).
10. according to any described method in claim 1-9, it is characterised in that：The cotton test tube seedling is No. 35 examinations of middle cotton
Pipe seedling or other kind test tube seedlings；
The stock that the grafting is used is sea island cotton seedling or the strong cotton variety of other well developed root systems, resistance of reverse.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710028477.4A CN106688589B (en) | 2017-01-16 | 2017-01-16 | Cotton test tube seedling trench method for transplanting |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710028477.4A CN106688589B (en) | 2017-01-16 | 2017-01-16 | Cotton test tube seedling trench method for transplanting |
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| Publication Number | Publication Date |
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| CN106688589A true CN106688589A (en) | 2017-05-24 |
| CN106688589B CN106688589B (en) | 2019-11-15 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201710028477.4A Expired - Fee Related CN106688589B (en) | 2017-01-16 | 2017-01-16 | Cotton test tube seedling trench method for transplanting |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107950340A (en) * | 2017-11-01 | 2018-04-24 | 河北省农林科学院遗传生理研究所 | A kind of cotton test tube method for transplanting of high-survival rate |
| CN109804881A (en) * | 2018-11-22 | 2019-05-28 | 安徽省农业科学院棉花研究所 | A kind of cotton field-transplanting wintering method |
Citations (4)
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| CN1685795A (en) * | 2005-04-30 | 2005-10-26 | 山西省农业科学院棉花研究所 | Substrate and method of cotton regeneration test tube seedling direct transplantation |
| CN1985573A (en) * | 2006-12-30 | 2007-06-27 | 中国农业科学院棉花研究所 | Non-embryo transgenic process for preparing transgenic cotton |
| CN103782811B (en) * | 2014-02-26 | 2015-09-02 | 武汉市农业科学研究所 | A kind of watermelon plantlet in vitro test tube micro-grafting method |
| CN105103972A (en) * | 2015-09-25 | 2015-12-02 | 山西省农业科学院棉花研究所 | Temperature-controlled grafting method for regenerated plants of transgenic cotton |
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2017
- 2017-01-16 CN CN201710028477.4A patent/CN106688589B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1685795A (en) * | 2005-04-30 | 2005-10-26 | 山西省农业科学院棉花研究所 | Substrate and method of cotton regeneration test tube seedling direct transplantation |
| CN1985573A (en) * | 2006-12-30 | 2007-06-27 | 中国农业科学院棉花研究所 | Non-embryo transgenic process for preparing transgenic cotton |
| CN103782811B (en) * | 2014-02-26 | 2015-09-02 | 武汉市农业科学研究所 | A kind of watermelon plantlet in vitro test tube micro-grafting method |
| CN105103972A (en) * | 2015-09-25 | 2015-12-02 | 山西省农业科学院棉花研究所 | Temperature-controlled grafting method for regenerated plants of transgenic cotton |
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| 吕少华 等: "《花卉种苗学》", 31 August 2009, 中国林业出版社 * |
| 林君: "陆地棉体细胞胚胎发生和植株再生体系的建立", 《中国优秀博硕士学位论文全文数据库（硕士） 农业科技辑》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107950340A (en) * | 2017-11-01 | 2018-04-24 | 河北省农林科学院遗传生理研究所 | A kind of cotton test tube method for transplanting of high-survival rate |
| CN109804881A (en) * | 2018-11-22 | 2019-05-28 | 安徽省农业科学院棉花研究所 | A kind of cotton field-transplanting wintering method |
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| CN106688589B (en) | 2019-11-15 |
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Granted publication date: 20191115 Termination date: 20220116 |