CN1685795A - Substrate and method of cotton regeneration test tube seedling direct transplantation - Google Patents
Substrate and method of cotton regeneration test tube seedling direct transplantation Download PDFInfo
- Publication number
- CN1685795A CN1685795A CN 200510012491 CN200510012491A CN1685795A CN 1685795 A CN1685795 A CN 1685795A CN 200510012491 CN200510012491 CN 200510012491 CN 200510012491 A CN200510012491 A CN 200510012491A CN 1685795 A CN1685795 A CN 1685795A
- Authority
- CN
- China
- Prior art keywords
- matrix
- test tube
- regeneration test
- tube seedling
- cotton
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)
Abstract
A matrix for directly transplanting the regenerative test-tube plantlet of cotton is prepared from the sand and coal ash taken from chimney in the ration of 1:(0.5-1) through mixing and high-pressure sterilizing. Its transplanting method includes loading said matrix in a plastic cup with holes on its bottom, transplanting the test-tube plantlet in it, covering it, immersing in the nutritive rooting liquid, putting it in a green house, supplementing water once every week until the fibrous roots and euphylises are grown up, transplanting it along with said matrix in flowerpot, growing new euphylises, and transplanting it along with the soil in field.
Description
Technical field
The present invention relates to the transplantation technique of cotton regeneration test tube seedling, the particularly used matrix of cotton regeneration test tube seedling direct transplantation and the method for direct transplanting are specially the matrix and the method for cotton regeneration test tube seedling direct transplantation.
Background technology
Mainly contain direct transplanting and graft and transplantation in the transplanting of northern China cotton regeneration test tube seedling.Usually direct transplanting is to be matrix with the sterile soil, promptly transplants in sterile soil.Because northern China is dry, the cotton in seedling stage damping off takes place serious, in sterile soil, transplant, because poor aeration, thereby easy mouldy the dying of test-tube plantlet root, transplanting survival rate is almost nil.The cotton test-tube seedling transplanting was mainly taked the method for grafting in recent years, but the cotton rootstock seedling November in winter of northern China between December, owing to easy infection damping off germ grows badly, can not carry out grafting.Way commonly used at present is to carry out Nan Fan, promptly carries out the grafting of regeneration test tube seedling in Hainan.This has influenced the process that the cotton test-tube plantlet enters the land for growing field crops to a great extent, has restricted the process of the utilization and the tissue culture work of transgenic cotton plant to a great extent.
Summary of the invention
The present invention is directed to the cotton regeneration test tube seedling graft and transplantation easily damping off takes place and influences transplanting survival rate and the low problem of direct transplanting survival rate at the northern area of China rootstock seedling in winter, a kind of matrix that is applicable to direct transplanting at first is provided, and next provides a kind of method of utilizing this matrix direct transplanting.
The present invention adopts following technical scheme to realize: a kind of matrix of cotton regeneration test tube seedling direct transplantation, this matrix by sand and chimney coal ash in 1: the ratio mixing of 0.5-1 is carried out making behind the autoclaving again.
Selecting materials of this matrix is to draw on the basis of a large amount of experiments, its uniqueness of selecting materials, and not only air permeability is good, preserves moisture, is incubated, and contain the needed mineral matter element of a large amount of cotton growths.It is used for the direct transplanting of cotton regeneration test tube seedling, can greatly improve the transplanting survival rate of test-tube plantlet.
Utilize above-mentioned matrix to carry out the method for cotton regeneration test tube seedling direct transplantation: by sand and chimney coal ash in 1: the ratio of 0.5-1 is mixed and is carried out making matrix behind the autoclaving again; Matrix is put into the plastic cup that the bottom has aperture, the cotton regeneration test tube seedling of preparing to transplant is taken out and washes off medium from cultivate vessel, direct transplanting is gone in the matrix in the plastic cup, and buckle the transparent plastic cup, the plastic cup immersion that the bottom is had aperture fills 30-60mg/LIBA (indolebutyric acid), the mixing of 30-60mg/LNAA (methyl) and 0.06-0.13% potassium permanganate is taken root in the basin of nutrient solution, make it suction moisture, put into temperature 20-28 ℃ then, air humidity 30%-70%, have in the greenhouse of sufficient natural lighting, replenish a water weekly, put seedling (plastic cup that is about to be buckled in top staggers gradually and exposes the slit) after 10 days gradually, put seedling (removing the upper plastic cup) in 30 days fully; After treating that cotton regeneration test tube seedling grows new fibrous root and true leaf, together be transplanted in the big flowerpot of containing soil, when regeneration test tube seedling grows new true leaf once more in the flowerpot of Sheng soil after, regeneration test tube seedling gone into the land for growing field crops together with the basin soil mud together with matrix.
The nutrient solution of taking root also can directly water in matrix, but causes hardening of matrix easily like this, thereby influences the transplanting survival rate of test-tube plantlet.And the plastic cup that will have aperture immerses the nutrient solution of taking root, and makes the aperture of the nutrient solution of taking root by plastic cup enter matrix from bottom to up, can guarantee the loose of matrix, helps improving the transplanting survival rate of test-tube plantlet.
The method of this direct transplanting is selected reasonably take root nutrient solution and by transplanting the complete control measures in back (accurate Temperature and Humidity Control and special transplanting operation) of unique matrix, prescription for use, can make cotton regeneration test tube seedling survival rate increase substantially.The method of this direct transplanting is not only with low cost, and is simple and easy to do, and the transplanting survival rate height, and survival rate can reach about 75% behind the test tube seedling direct transplantation of robust growth.Promoted the cotton test-tube plantlet to enter the process in land for growing field crops and the utilization of transgenic cotton plant, the process of tissue culture work to a great extent, can promote the use of on a large scale aspect the cotton regeneration test tube seedling transplanting, be specially adapted to the transplanting of northern China cotton regeneration test tube seedling during winter.
Description of drawings
Fig. 1 transplants the back for cotton regeneration test tube seedling and has growth in the plastic cup (nutritive cube) of aperture in the bottom;
Fig. 2 transplants the growth of back in flowerpot for the cotton regeneration test tube seedling nutrition pot seedling;
Fig. 3 transplants the growth of back in flowerpot for the cotton regeneration test tube seedling nutrition pot seedling;
Embodiment
Sand and chimney coal ash carry out making matrix behind the autoclaving in the ratio mixing of 1: 1 (or 1: 0.5,1: 0.8) again.Described sand is the common sand of architectural use, preferred river sand (being the sand on limit, riverbank); Described chimney coal ash is exactly the thin black powder that chimney (as boiler chimney) volatilizes, and it particularly exists in the chimney of Industrial Boiler or civil heating boiler in a large number at chimney, very easily obtains and it contains the needed mineral matter element of a large amount of cotton growths.The sterilization of matrix can be under 121 ℃, and high pressure 30-40 minute, this was conventional sterilizing methods.
The matrix bottom of packing into is had in the disposal plastic cup of 3-4 aperture (or in nutritive cube of other bottom opening); Cotton regeneration test tube seedling is taken out from cultivate vessel, the medium on root and the plant is rinsed well, put into 0.1% liquor potassic permanganate and soaked 3-5 minute with running water; Direct transplanting is equipped with in the plastic cup of matrix, and buckles the transparent plastic cup; The plastic cup that will have aperture immerses the mixing fill 50mg/LIBA (indolebutyric acid), 50mg/LNAA (methyl) 0.1% potassium permanganate (or 30mg/LIBA (indolebutyric acid), 40mg/LNAA (methyl), 0.08% potassium permanganate or 40mg/LIBA (indolebutyric acid), 60mg/LNAA (methyl), 0.06% potassium permanganate or 60mgIBA (indolebutyric acid), 30mg/LNAA (methyl) 0.13% potassium permanganate) takes root in the basin of nutrient solution, makes it suction moisture content; The plastic cup that moves into test-tube plantlet and suction moisture content is put into screen tray with holes (to ventilate to guarantee the plastic cup bottom, prevent that test-tube plantlet from rotting root), put into temperature 20-28 ℃ (20 ℃ then, 23 ℃, 25 ℃, 28 ℃), air humidity 30%-70% (30%, 40%, 50%, 60%, 70%), have in the greenhouse of sufficient natural lighting (or in respective environment), water a water (preferably Xi Shi MS nutrient solution) weekly, put the seedling (plastic cup that is about to the to be buckled in top slit of staggering gradually after 10 days gradually, the slit of staggering is ascending), put seedling (removing the upper plastic cup) in 30 days fully; After treating that cotton regeneration test tube seedling grows new fibrous root and true leaf, together be transplanted in the big flowerpot of containing soil, when regeneration test tube seedling grows new true leaf once more in the flowerpot of Sheng soil after, regeneration test tube seedling gone into the land for growing field crops together with the basin soil mud together with matrix.
Claims (3)
1, a kind of matrix of cotton regeneration test tube seedling direct transplantation is characterized by: this matrix by sand and chimney coal ash in 1: the ratio of 0.5-1 is mixed and is carried out making behind the autoclaving again.
2, utilize matrix according to claim 1 to carry out the method for cotton regeneration test tube seedling direct transplantation, it is characterized by: by sand and chimney coal ash in 1: the ratio of 0.5-1 is mixed and is carried out making matrix behind the autoclaving again; Matrix is put into the plastic cup that the bottom has aperture, the cotton regeneration test tube seedling of preparing to transplant is taken out and washes off medium from cultivate vessel, direct transplanting is gone in the matrix in the plastic cup, and buckle the transparent plastic cup, the plastic cup immersion that the bottom is had aperture fills 30-60mg/L IBA (indolebutyric acid), the mixing of 30-60mg/LNAA (methyl) and 0.06-0.13% potassium permanganate is taken root in the basin of nutrient solution, make it suction moisture, put into temperature 20-28 ℃ then, air humidity 30%-70%, have in the greenhouse of sufficient natural lighting, replenish a water weekly, put seedling after 10 days gradually, put seedling in 30 days fully; After treating that cotton regeneration test tube seedling grows new fibrous root and true leaf, together be transplanted in the big flowerpot of containing soil, when regeneration test tube seedling grows new true leaf once more in the flowerpot of Sheng soil after, regeneration test tube seedling gone into the land for growing field crops together with the basin soil mud together with matrix.
3, the method for cotton regeneration test tube seedling direct transplantation as claimed in claim 2 is characterized by: the plastic cup that will move into test-tube plantlet and suction moisture content is put into screen tray with holes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100124912A CN1312981C (en) | 2005-04-30 | 2005-04-30 | Substrate and method of cotton regeneration test tube seedling direct transplantation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100124912A CN1312981C (en) | 2005-04-30 | 2005-04-30 | Substrate and method of cotton regeneration test tube seedling direct transplantation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1685795A true CN1685795A (en) | 2005-10-26 |
| CN1312981C CN1312981C (en) | 2007-05-02 |
Family
ID=35303837
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2005100124912A Expired - Fee Related CN1312981C (en) | 2005-04-30 | 2005-04-30 | Substrate and method of cotton regeneration test tube seedling direct transplantation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1312981C (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101773039B (en) * | 2010-01-28 | 2011-05-25 | 山西省农业科学院棉花研究所 | Transgenic cotton regenerated plant transplantation method |
| CN102057869B (en) * | 2009-11-18 | 2012-09-26 | 中国农业科学院棉花研究所 | Cotton regeneration seedling rooting method and special culture medium thereof |
| CN102972297A (en) * | 2012-12-05 | 2013-03-20 | 中国农业科学院生物技术研究所 | Method for cultivating regeneration plants of cotton |
| CN103477990A (en) * | 2013-10-15 | 2014-01-01 | 阮颖 | Method for rooting contaminated test-tube plantlets during plant tissue culture and application of method |
| CN104956930A (en) * | 2015-07-14 | 2015-10-07 | 山西省农业科学院棉花研究所 | Method for suspended transplantation of regenerated transgenic cotton plant |
| CN106688589A (en) * | 2017-01-16 | 2017-05-24 | 山西省农业科学院棉花研究所 | Cotton test tube seedling trench transplanting method |
| CN107950340A (en) * | 2017-11-01 | 2018-04-24 | 河北省农林科学院遗传生理研究所 | A kind of cotton test tube method for transplanting of high-survival rate |
-
2005
- 2005-04-30 CN CNB2005100124912A patent/CN1312981C/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102057869B (en) * | 2009-11-18 | 2012-09-26 | 中国农业科学院棉花研究所 | Cotton regeneration seedling rooting method and special culture medium thereof |
| CN101773039B (en) * | 2010-01-28 | 2011-05-25 | 山西省农业科学院棉花研究所 | Transgenic cotton regenerated plant transplantation method |
| CN102972297A (en) * | 2012-12-05 | 2013-03-20 | 中国农业科学院生物技术研究所 | Method for cultivating regeneration plants of cotton |
| CN103477990A (en) * | 2013-10-15 | 2014-01-01 | 阮颖 | Method for rooting contaminated test-tube plantlets during plant tissue culture and application of method |
| CN104956930A (en) * | 2015-07-14 | 2015-10-07 | 山西省农业科学院棉花研究所 | Method for suspended transplantation of regenerated transgenic cotton plant |
| CN106688589A (en) * | 2017-01-16 | 2017-05-24 | 山西省农业科学院棉花研究所 | Cotton test tube seedling trench transplanting method |
| CN106688589B (en) * | 2017-01-16 | 2019-11-15 | 山西省农业科学院棉花研究所 | Cotton test tube seedling trench method for transplanting |
| CN107950340A (en) * | 2017-11-01 | 2018-04-24 | 河北省农林科学院遗传生理研究所 | A kind of cotton test tube method for transplanting of high-survival rate |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1312981C (en) | 2007-05-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1312981C (en) | Substrate and method of cotton regeneration test tube seedling direct transplantation | |
| CN103975836B (en) | A kind of water planting Chinese cymbidium root induction method | |
| CN109618932B (en) | Method for inducing adventitious buds of rhododendron dauricum and regenerating plants | |
| WO2020107888A1 (en) | Ex vitro rooting method for acer pentaphyllum tissue culture | |
| CN104025988A (en) | Soilless cultivation method of gingko plants | |
| CN104542281A (en) | Tissue culture and propagation method of viburnum tinus | |
| CN103548660B (en) | Plant seedling pot allowing automatic liquid absorption | |
| CN103039366A (en) | Industrial production method of mycorrhizal seedlings of Changbai Mountain rhododendron plants | |
| CN103155869A (en) | Sweet cherry rootstock Colt tissue culture method | |
| CN106818432A (en) | A kind of method of utilization matrix culture and improvement matrimony vine seedling salt tolerance | |
| CN101313661A (en) | Multitudinous transplant method for woody flower group cultivated seedling | |
| CN101112173B (en) | Tissue culture propagating method for heavy metal super-enriched thlaspi caerulescens | |
| CN115589947A (en) | Tissue culture and rapid propagation method of salix matsudana and application thereof | |
| CN104686336A (en) | Tissue culture rapid propagation method of ailanthus altissima | |
| JP3499479B2 (en) | Mycorrhizal seedling preparation and artificial cultivation of mycorrhizal mushrooms | |
| CN106234229A (en) | A kind of method that Fructus Colocasiae Esculentae tissue cultured seedling is heeled in | |
| CN113317158A (en) | Seedling raising method for improving rapid seedling formation of Australian water lily seed seedlings | |
| CN106358646A (en) | Chrysanthemum cutting seedling cultivation method | |
| JPS63297304A (en) | Method for culturing and cultivating araceous plant | |
| CN112772415A (en) | Method for rapidly propagating banana seedlings | |
| CN107278895B (en) | Industrial tissue culture and rapid propagation technology of tamarix chinensis | |
| CN110583486A (en) | Method for cultivating high-stress-resistance broussonetia papyrifera | |
| CN106171971B (en) | A kind of method of clove leaf honeysuckle tissue-culturing rapid propagation | |
| LU503970B1 (en) | Hardening-off and transplanting method for seedlings of transgenic resistant plants of castor | |
| CN110741902B (en) | Simple seedling culture method for royal lotus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20070502 |