CN104956930A - Method for suspended transplantation of regenerated transgenic cotton plant - Google Patents

Abstract

The invention discloses a method for suspended transplantation of a regenerated transgenic cotton plant. The method for suspended transplantation of the regenerated transgenic cotton plant comprises the following steps that 1, a cotton rootstock seedling is grown in a container; 2, a transgenic cotton seedling is grafted onto the rootstock seedling; 3, a support is inserted into the root of the rootstock seedling; 4, a grafted seedling is wrapped by a plastic film at the position close to the support, above the rootstock seedling and below the grafted seedling; 5, after seven to eight days, ventilation and dehumidification are conducted gradually, the container and the plastic film are removed, and then transplantation can be conducted. According to the method for suspended transplantation of the regenerated transgenic cotton plant, a suspended grafting method is adopted, in this way, the environment humidity can be lowered for the grafted seedling which is just grafted for seven days, the problem that the root of the rootstock seedling or the grafted seedling can get rotten easily in the high humidity grafting environment during grafting when the grafted seedling is grafted at a high temperature in summer is solved, the survival rate of grated seedlings can reach 95%, and the method is simple.

Description

The unsettled grafting of a kind of transgenic cotton regenerated plant

Technical field

The present invention relates to transgene cotton technical field, be specifically related to the unsettled grafting of a kind of transgenic cotton regenerated plant.

Background technology

Adopt agrobacterium-mediated transformation to obtain transgenosis regeneration strain very not easily by Studies of Transfer of Alien Genes Into Receptors cotton, and confirm that foreign gene imports the regeneration strain rareer (conversion ratio of general agrobacterium-mediated transformation is all very high more than 40%) in acceptor really through Molecular Detection.Therefore, the technical research improving the transplanting success of transgenosis regeneration strain does not pause in transgene cotton field always.At present, the conventional method for transplanting of main employing and grafting.Maybe eliminate gradually with constant temperature and humidity climatic cabinate because conventional method for transplanting survival rate is too low; Grafting has continued to use nearly ten years, and method is also in continuous renewal, simplification (table 1).

Table 1 correlation technique granted patent

Existing transplantation technique has two kinds: (1) conventional method for transplanting, (2) low temperature and high relative humidity method for transplanting;

(1) conventional method for transplanting: transgenic cotton regenerated plant take root seedling to open between bottle cap → cultivation hardening 1 ~ 2 day → wash away cotton shoot root portion medium → plant to be equipped with in the soil of sterilizing or the container of sand → disposable water permeable → move in greenhouse or solarium and yield positive results to cotton.(2) low temperature and high relative humidity method for transplanting: transgenic cotton regenerated plant take root seedling open hardening between bottle cap → cultivation 1 ~ 2 day → wash away root medium → plant to be equipped with in the soil of sterilizing or the container of sand → disposable water permeable → put into humidity 88% ~ 90%, 20 DEG C ~ 22 DEG C incubators to cultivate 10 days ~ 15 days → greenhouse that moves into normal condition and survive cotton and yield positive results.

Existing graft technology:

Grafting (now the normal method used): on (cotton) stock that greenhouse or solarium are planted transgenic cotton regenerated plant grafting in basin alms bowl, then plastic film (or special container) is used to wrap up whole cotton seedling, progressively leak informaton after 7 days, finally take down plastic film or the container of parcel.

Existing graft technology common problem is in summer high temperature season, grafting and stock all will wrap up, and be connected with ground, very easily occur stock rot or grafting rot, therefore summer various transgenosis regenerated plant transplantation method survival rate do not reach desirable result all the time.

Summary of the invention

The object of the invention is to provide the unsettled grafting of a kind of transgenic cotton regenerated plant, and it can solve problem existing in background technology effectively.

The unsettled grafting of a kind of transgenic cotton regenerated plant, it comprises the following steps:

1), plant cotton rootstock seedling in container;

2), by the grafting of transgenic cotton flower seedling on stock;

3), supporter is inserted at rootstock seedling root;

4), grafting is wrapped up near supporter plastic foil in grafting bottom, rootstock seedling top;

5), after 7-8 days, progressively leak informaton and dry, remove container and plastic foil, can transplant.

Owing to have employed above technical scheme, the present invention has following beneficial effect: adopt unsettled grafting, the ambient humidity of grafting during firm grafting 7 days can be reduced, solve grafting when the grafting of summer high temperature period due to the high humidity bad border of grafting in stock root or the extremely perishable difficult problem of grafting, make its survival rate more than 95%, and method is simple.

Embodiment

Embodiment

The unsettled grafting of a kind of transgenic cotton regenerated plant, it comprises the following steps:

1), plant cotton rootstock seedling in container;

2), by the grafting of transgenic cotton flower seedling on stock;

3), supporter is inserted at rootstock seedling root;

4), grafting is wrapped up near supporter plastic foil in grafting bottom, rootstock seedling top;

5), after 7-8 days, progressively leak informaton and dry, remove container and plastic foil, can transplant.

Owing to have employed above technical scheme, the present embodiment has following beneficial effect: adopt unsettled grafting, the ambient humidity of grafting during firm grafting 7 days can be reduced, solve grafting when the grafting of summer high temperature period due to the high humidity bad border of grafting in stock root or the extremely perishable difficult problem of grafting, make its survival rate more than 95%, and method is simple.

Last it is noted that above embodiment is only in order to illustrate technical scheme of the present invention, be not intended to limit; Although with reference to previous embodiment to invention has been detailed description, those of ordinary skill in the art should be appreciated that it still can be modified to the technical scheme described in foregoing embodiments, or carries out equivalent replacement to wherein portion of techniques feature; And these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of various embodiments of the present invention technical scheme.

Claims (1)

1. the unsettled grafting of transgenic cotton regenerated plant, is characterized in that it comprises the following steps:

1), plant cotton rootstock seedling in container;

2), by the grafting of transgenic cotton flower seedling on stock;

3), supporter is inserted at rootstock seedling root;

4), grafting is wrapped up near supporter plastic foil in grafting bottom, rootstock seedling top;

5), after 7-8 days, progressively leak informaton and dry, remove container and plastic foil, can transplant.