CN1312981C - Substrate and method of cotton regeneration test tube seedling direct transplantation - Google Patents

Abstract

The invention relates to a substrate and a method for direct transplanting of cotton regenerated test-tube seedlings, and relates to the transplanting technology of cotton regenerated test-tube seedlings. The matrix is prepared by mixing sand and chimney coal ash at a ratio of 1:0.5-1 and then autoclaving. The method of direct transplanting is to put the substrate into a plastic cup with a small hole at the bottom, transplant the test-tube seedlings into the plastic cup, buckle the transparent plastic cup, immerse in the rooting nutrient solution, make it absorb enough water, and then put it into the plastic cup. In a greenhouse with a temperature of 20-28°C, an air humidity of 30%-70%, and sufficient natural light, add water once a week, gradually release the seedlings after 10 days, and completely release the seedlings after 30 days; wait for the cotton regeneration test tube seedlings to grow new fibrous roots After the true leaves are removed, they are transplanted together with the substrate into a large flower pot filled with soil, and when the regenerated test-tube plantlet grows new true leaves again in the flower pot filled with soil, the regenerated test-tube plantlet is moved into the field together with the pot soil; The planting survival rate reaches about 75%, which is especially suitable for transplanting cotton regeneration test tube seedlings in winter in northern China.

Description

Substrate and method for direct transplanting of cotton regenerated test-tube plantlets

technical field

The invention relates to the transplanting technology of cotton regenerated test-tube seedlings, in particular to a substrate used for direct transplanting of cotton regenerated test-tube seedlings and a direct transplanting method, in particular to a substrate and a method for direct transplanting of cotton regenerated test-tube seedlings.

Background technique

Transplanting of cotton regeneration test tube seedlings mainly includes direct transplanting and grafting transplanting in northern my country. Usually direct transplanting is based on sterile soil, that is, transplanting in sterile soil. Due to the dry climate in northern my country, the blight of cotton seedlings occurs seriously. When transplanted in sterile soil, due to poor ventilation, the roots of test tube seedlings are prone to mold and die, and the survival rate of transplanting is almost zero. In recent years, the transplanting of cotton test-tube seedlings mainly adopts the method of grafting, but cotton rootstock seedlings grow poorly due to the susceptibility to bacterial blight and cannot be grafted between November and December in the winter of northern my country. The commonly used method at present is to carry out southern propagation, promptly carry out the grafting of regenerated test-tube seedlings in Hainan. This has affected the process of cotton test-tube seedlings entering the field to a large extent, and has restricted the utilization of cotton transgenic plants and the process of tissue culture work to a large extent.

Contents of the invention

The present invention aims at the problem that the grafting and transplanting of cotton regenerated test-tube seedlings is prone to blight in winter in northern my country, which affects the survival rate of transplanting and the low survival rate of direct transplanting. Firstly, it provides a substrate suitable for direct transplanting. Secondly, a method of directly transplanting using the matrix is given.

The present invention is realized by adopting the following technical scheme: a substrate for direct transplanting of cotton regeneration test-tube seedlings, which is prepared by mixing sand and chimney coal ash at a ratio of 1:0.5-1, followed by high-pressure sterilization.

The material selection of the matrix is obtained on the basis of a large number of experiments. The material selection is unique, not only has good ventilation, moisturizing and heat preservation, but also contains a large amount of mineral elements needed for cotton growth. The method is used for direct transplanting of cotton regeneration test-tube seedlings, and can greatly improve the transplanting survival rate of test-tube seedlings.

The method of directly transplanting cotton regeneration test-tube seedlings using the above-mentioned substrate: mix sand and chimney coal ash in a ratio of 1:0.5-1 and then carry out high-pressure sterilization to obtain the substrate; put the substrate into a plastic with small holes at the bottom In the cup, take out the cotton regeneration test tube seedlings to be transplanted from the culture vessel and wash off the culture medium, transplant them directly into the matrix in the plastic cup, and buckle the transparent plastic cup, put the plastic cup with a small hole in the bottom Immerse it in a pot filled with a mixed rooting nutrient solution of 30-60mg/L IBA (indole butyric acid), 30-60mg/L NAA (naphthalene acetic acid) and 0.06-0.13% potassium permanganate to make it absorb enough water, Then put it into a greenhouse with a temperature of 20-28°C, an air humidity of 30%-70%, and sufficient natural light, replenish water once a week, and gradually release the seedlings after 10 days (the plastic cups that are about to be buckled on the top are gradually staggered to expose gaps), Put the seedlings completely in 30 days (remove the upper plastic cup); after the cotton regeneration test-tube seedlings grow new fibrous roots and true leaves, they are transplanted together with the substrate into a large flower pot filled with soil. After new true leaves grow again, the regenerated test-tube seedlings are moved into the field together with the potting soil.

The rooting nutrient solution can also be directly poured on the substrate, but it is easy to cause the compaction of the substrate like this, thereby affecting the transplanting survival rate of the test-tube plantlets. And immerse the plastic cup with aperture into rooting nutrient solution, make rooting nutrient solution enter substrate from bottom to top through the aperture of plastic cup, can guarantee the looseness of substrate, help to improve the transplanting survival rate of test-tube seedling.

The direct transplanting method selects a unique substrate, a rooting nutrient solution with a reasonable formula, and through a complete set of management measures (accurate temperature and humidity control and special transplanting procedures) after transplanting, the survival rate of cotton regeneration test tube seedlings can be improved. substantial improvement. The direct transplanting method is not only low in cost, simple and easy to implement, but also has a high transplanting survival rate, and the survival rate of vigorously growing test-tube seedlings after direct transplanting can reach about 75%. To a large extent, it has promoted the process of cotton test-tube seedlings entering the field, the utilization of cotton transgenic plants, and the process of tissue culture. It can be widely used in the transplanting of cotton regeneration test-tube seedlings, and is especially suitable for cotton regeneration in winter in northern my country. Transplanting of test tube seedlings.

Description of drawings

Fig. 1 has the growth in the plastic cup (nutritional bowl) of aperture at the bottom after the cotton regeneration test-tube seedling is transplanted;

Fig. 2 is the growth in the flower pot after the cotton regeneration test-tube plantlet nutrient pot seedling is transplanted;

Fig. 3 is the growth in the flower pot after the cotton regeneration test-tube plantlet nutrient pot seedling is transplanted;

Detailed ways

Sand and chimney coal ash were mixed at a ratio of 1:1 (or 1:0.5, 1:0.8) and then autoclaved to prepare the matrix. Described sand is the common sand that uses on building, preferred river sand (the sand on the river bank); Described chimney coal ash is exactly the fine black powder that chimney (as boiler chimney) volatilizes, and it especially in chimney There are a lot of chimneys of industrial boilers or civil heating boilers, which are very easy to get and contain a lot of mineral elements needed for cotton growth. The matrix can be sterilized at 121°C for 30-40 minutes under high pressure, which is a conventional sterilization method.

Put the matrix into a disposable plastic cup with 3-4 small holes at the bottom (or other nutritional pots with holes at the bottom); take out the cotton regeneration test tube seedlings from the culture vessel, and use tap water to soak the roots and plants Rinse the culture medium and soak it in 0.1% potassium permanganate solution for 3-5 minutes; directly transplant it into a plastic cup with a substrate, and buckle the transparent plastic cup; immerse the plastic cup with a small hole in the container 50mg/L IBA (indolebutyric acid), 50mg/L NAA (naphthaleneacetic acid), 0.1% potassium permanganate (or 30mg/L IBA (indolebutyric acid), 40mg/L NAA (naphthaleneacetic acid), 0.08% Potassium permanganate or 40mg/L IBA (indole butyric acid), 60mg/L NAA (naphthalene acetic acid), 0.06% potassium permanganate or 60mg IBA (indole butyric acid), 30mg/L NAA (naphthalene acetic acid) 0.13 %potassium permanganate) in the basin of the mixed rooting nutrient solution to make it absorb enough water; the plastic cup that will be moved into the test tube seedling and absorb enough water is put into the sieve tray with holes (to ensure that the bottom of the plastic cup is ventilated, prevent test-tube seedlings from rotten), and then put them into a temperature of 20-28°C (20°C, 23°C, 25°C, 28°C), and an air humidity of 30%-70% (30%, 40%, 50%, 60%, 70% %), in a greenhouse with sufficient natural light (or in a corresponding environment), pour water (preferably diluted MS nutrient solution) once a week, and gradually release the seedlings after 10 days (the plastic cups that are about to be buckled on the upper part are gradually staggered, Stagger the gap from small to large), put the seedlings completely in 30 days (remove the upper plastic cup); after the cotton regeneration test tube seedlings grow new fibrous roots and true leaves, they are transplanted together with the substrate into a large flower pot filled with soil, when After the regenerated test-tube plantlet grows new true leaves again in the flowerpot filled with soil, the regenerated test-tube plantlet is moved into the field together with the pot soil.

Claims (3)

1. A substrate for direct transplanting of cotton regeneration test-tube seedlings is characterized in that: the substrate is made by mixing sand and chimney coal ash in a ratio of 1: 0.5-1 and then autoclaving. 2. Utilize the method for direct transplanting of cotton regeneration test-tube seedlings using the matrix as claimed in claim 1, characterized in that: the matrix is prepared by mixing sand and chimney coal ash in a ratio of 1: 0.5-1 and then autoclaving; Put the substrate into a plastic cup with a small hole at the bottom, take out the cotton regeneration test-tube seedlings to be transplanted from the culture vessel and wash off the medium, transplant them directly into the substrate in the plastic cup, and buckle the transparent plastic Cup, immerse the plastic cup with a small hole in the bottom into the mixed rooting nutrient solution containing 30-60mg/L IBA (indole butyric acid), 30-60mg/LNAA (naphthalene acetic acid) and 0.06-0.13% potassium permanganate Put it in a pot to make it absorb enough water, and then put it in a greenhouse with a temperature of 20-28°C, an air humidity of 30%-70%, and sufficient natural light. Water is added once a week, and the seedlings are gradually released after 10 days. Put the seedlings; after the regeneration test-tube seedlings of cotton grow new fibrous roots and true leaves, they are transplanted together with the substrate into a large flowerpot filled with soil; Move the regenerated test-tube plantlets together with the potting soil into the field. 3. The method for directly transplanting cotton regenerated test-tube seedlings as claimed in claim 2, characterized in that: the plastic cup that has been moved into the test-tube seedlings and absorbs enough water is put into the sieve tray with holes.