CN106818432A - A kind of method of utilization matrix culture and improvement matrimony vine seedling salt tolerance - Google Patents

A kind of method of utilization matrix culture and improvement matrimony vine seedling salt tolerance Download PDF

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Publication number
CN106818432A
CN106818432A CN201611093466.6A CN201611093466A CN106818432A CN 106818432 A CN106818432 A CN 106818432A CN 201611093466 A CN201611093466 A CN 201611093466A CN 106818432 A CN106818432 A CN 106818432A
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matrimony vine
soil
content
vine seedling
pouring
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CN106818432B (en
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朱小梅
洪立洲
邢锦城
温祝桂
刘冲
赵宝泉
丁海荣
董静
王茂文
刘兴华
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Jiangsu Institute of Coastal Area Agricultural Sciences
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Jiangsu Institute of Coastal Area Agricultural Sciences
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/08Immunising seed
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/04Electric or magnetic or acoustic treatment of plants for promoting growth
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D1/00Fertilisers containing potassium
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G1/00Mixtures of fertilisers belonging individually to different subclasses of C05
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/20Liquid fertilisers
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/14Measures for saving energy, e.g. in green houses

Abstract

The present invention relates to a kind of method of utilization matrix culture and improvement matrimony vine seedling salt tolerance.Directly sowed for matrimony vine or cuttage in salt content on 2 ‰~6 ‰ salinized soil, matrimony vine emergence rate and the relatively low problem of survival rate have invented a kind of method of utilization matrix culture and improvement matrimony vine seedling salt tolerance.Peat, vermiculite, perlite, straw ash are sowed at after matrimony vine seed is sterilized by volume in 3: 3: 3: 1 matrix for mixing, pour the mud-flat soil leaching liquor similar to ion composition in region to be transplanted, and salt stress concentration is stepped up from low to high, when matrimony vine seedling it is long to 6~9 leaves when transplant on the salinized soil that salt content is 2 ‰~6 ‰, can make matrimony vine seedling percent improve 8.1%~16.3%.

Description

A kind of method of utilization matrix culture and improvement matrimony vine seedling salt tolerance
Technical field
The present invention relates to a kind of method of utilization matrix culture and improvement matrimony vine seedling salt tolerance, belong to plant cultivation technology neck Domain.
Background technology
Matrimony vine is the perennial machaka of Solanaceae Lycium, and its young stem and leaf can for vegetable consumption, and the fruit of Chinese wolfberry is important medicine With resource, with economic worth higher, Coastal beach can be introduced and developed and utilized.Matrimony vine modes of reproduction is various, can both lead to Seminal propagation is crossed, can also be bred by modes such as cuttages, if but directly sowing or cuttage, on salt content soil higher, go out Seedling rate is relatively low with survival rate, and this not only wastes manpower and materials, while returning production causes heavy losses.
The content of the invention
The present invention directly sowed for matrimony vine or cuttage in beach salt content on 2 ‰~6 ‰ soil, matrimony vine emerges Rate and the relatively low problem of survival rate, have invented a kind of method of utilization matrix culture and improvement matrimony vine seedling salt tolerance, so that Survival rate of the matrimony vine seedling on mud-flat soil improves 8.1%~16.3%.
A kind of method of utilization matrix culture and improvement matrimony vine seedling salt tolerance, comprises the following steps:
(1) matrimony vine seedling medium is 3: 3: 3: 1 mixing by volume of peat, vermiculite, perlite, straw ash, and sterilized 121 DEG C~124 DEG C high-temperature sterilizations of pot uniformly insert plastics cave dish after processing 30 minutes;
(2) plastics cave dish for using, cave number 5 × 10, disk body 54cm long, 28cm wide, cave depth 5cm;Hole tray transfers a shallow mouth Pallet, in case nutrient solution is lost in;
(3) matrimony vine seed is first rinsed 8 times through after 75% alcohol disinfecting 2~3 minutes with running water, then uses 2%NaClO solution Sterilization 5~6 minutes and with running water rinse 8 times after, dry;Matrix is first inserted in hole tray during sowing, is put down substantially with hole tray mouth Qi Shi, flattens, and every bunch planting kind 5~6, then the one layer of 0.3cm thick matrix lid that tiles crosses seed, and culture is put into after pouring nutrient solution Cultivated in case.The thinning of neat after seedling once, 2 plants of matrimony vine seedling is stayed per cave;
(4) extraction soil is the beach salty soil from jiangsu coast collection.Compare 1 with Tu Shui in 25 DEG C of constant temperature oscillators : after 5 vibration extractions 24 hours, pumping filtering obtains the soil extraction that Tu Shui compares 1: 5.Dilution one times and twice after respectively 1: 10 and 1: 20 soil extraction;
(5) soil extraction that concentration is for 1: 20 is after planting first poured, and need to be irrigated during first time nutrient solution, then Poured once every 2~3 days, often each irrigation amount of disk is 250mL, while adding the NH of 1mol/L4H2PO4Solution 0.5mL, 15 The soil extraction of pouring 1: 10 is changed to after it, it is same pour 15 days after, then be changed to the soil extraction of pouring 1: 5, pour 30 After it, transplanted when the number of blade is 6~9 leaves;
(6) domestication illumination box design temperature is 25 DEG C, and intensity of illumination is 12h/d;
(7) hole tray, when outside air temperature reaches 15 DEG C, is moved to outdoor refining by appropriate hardening together with shallow mouth pallet daily 3~6h of seedling, nursery is transplanted on the salinized soil that salt content is 2 ‰~6 ‰ after 60 days together with matrix, and pours water.
Straw ash described in above-mentioned steps (1) is the stalk ash content of generation after biomass power plants generating electricity, its main component P2O5 Content is 0.30%~0.39%, K2O content is 2.75%~2.84%.
1: 5 soil extraction pH described in above-mentioned steps (4) is that 7.55~7.78, alkaline hydrolysis nitrogen content is 38.2mg/kg ~41.5mg/kg, available phosphorus content are 50.1mg/kg~54.7mg/kg, effective potassium content is 376.9mg/kg~383.5mg/ Kg, the content of organic matter are 3.98g/kg~4.16g/kg, water soluble salt total ion concentration is 14.7g/kg~15.9g/kg, Cl contents It is 5.13g/kg~5.76g/kg.
Compared with the prior art the present invention, has the following advantages that:
(1) use substrate seedling and nutrient solution can provide enough nutrition for matrimony vine seedling stage;(2) seedling medium warp Cross high temperature sterilization treatment, matrimony vine seed is by after 75% alcohol and the treatment of 2%NaClO solution, it is to avoid in illumination cultivation environment The growth of lower bacterium mould, for the robust growth of matrimony vine seedling provides good condition;(3) incubator illumination and temperature adjustment are Chinese holly Qi optimum growing condition, can promote the fast-growth of matrimony vine seedling;(4) nutrient solution is the soil extract of beach salty soil Liquid, the salt ion composition to matrimony vine seedling region to be transplanted is similar, and it is foster to be properly added nitrogen, phosphorus according to the nutrient of leaching liquor composition Point, rapidly adapt to extraneous soil environment after matrimony vine seedling replanting can be made;(5) leaching liquor concentration is carried gradually from low-level to high level Rise, matrimony vine seedling can be made progressively to adapt to salt stress environment, and can in time observe life of the matrimony vine seedling under different leaching liquor concentration Situation long;(6) matrimony vine seedling is transferred to outdoor appropriate hardening after outside air temperature reaches more than 15 DEG C, can better adapts to it outer Boundary's temperature and illumination condition.Matrimony vine seed or cuttage seeding is the method overcome to be emerged on 2 ‰~6 ‰ salinized soil in salt content Rate or the relatively low problem of survival rate, are that establishing in large scale of the matrimony vine on beach salinized soil creates advantage.
Brief description of the drawings:
Fig. 1 represents influence of the different substrates treatment to matrimony vine Seedling Biomass.
Fig. 2 represents influence of the different leaching liquor treatment to matrimony vine Seedling Biomass.
Fig. 3 represents influence of the different leaching liquor treatment to matrimony vine seedling SPAD values.
Fig. 4 represent described in step 5 leaching liquor treatment after, on the salinized soil of matrimony vine seedling replanting to 2 ‰~4 ‰ into Motility rate.
Fig. 5 represent described in step 5 leaching liquor treatment after, on the salinized soil of matrimony vine seedling replanting to 4 ‰~6 ‰ into Motility rate.
Different substrates processing parameter is as follows in Fig. 1:
Treatment I:Peat, vermiculite, perlite, straw ash volume ratio are 1: 1: 1: 1;
Treatment II:Peat, vermiculite, perlite, straw ash volume ratio are 3: 3: 3: 2;
Treatment III:Peat, vermiculite, perlite, straw ash volume ratio are 3: 3: 3: 1;
Treatment IV:Peat, vermiculite, perlite, straw ash volume ratio are 1: 1: 1: 0.
Different leaching liquor processing parameters are as follows in Fig. 2, Fig. 3:
Treatment A:The NH of pure water+1mol/L4H2PO4Solution is poured;
Treatment B:The NH of 1: 20 soil extraction+1mol/L4H2PO4Solution is poured;
Treatment C:The NH of 1: 10 soil extraction+1mol/L4H2PO4Solution is poured;
Treatment D:The NH of 1: 5 soil extraction+1mol/L4H2PO4Solution is poured;
Treatment E:Poured by the soil extraction described in step 5.
1,2,3,4 represent number of repetition in Fig. 4, Fig. 5.
Specific embodiment
Embodiment 1
(1) matrimony vine seedling medium peat, vermiculite, perlite, straw ash are well mixed by 3: 3: 3: 1 volume ratio, warp 121 DEG C~124 DEG C high-temperature sterilizations of autoclave are processed 30 minutes, and uniformly insert plastics cave dish;Wherein main component in straw ash P2O5Content is 0.30%, K2O content is 2.75%;(2) the plastics cave dish caves number 5 × 10 for using, disk body 54cm long is wide 28cm, cave depth 5cm;Hole tray transfers one shallow mouthful of pallet, in case nutrient solution is lost in;(3) matrimony vine seed first through 75% alcohol disinfecting 2~ After 3 minutes with running water rinse 8 times, then with 2%NaClO solution disinfections 5~6 minutes and with running water flushing 8 times after, dry; Matrix is first inserted in hole tray during sowing, when being substantially flush with hole tray mouth, is flattened, every bunch planting kind 5~6, then tile one layer 0.3cm thick matrix lid crosses seed, is put into culture in incubator.The thinning of neat after seedling once, 2 plants of matrimony vine seedling is stayed per cave;(4) soak Drawings soil is the beach salty soil from jiangsu coast collection, and 1: 5 vibration extraction 24 is compared with Tu Shui in 25 DEG C of constant temperature oscillators After hour, pumping filtering obtains the soil extraction that Tu Shui compares 1: 5.Soil extraction pH is that 7.78, alkaline hydrolysis nitrogen content is 41.5mg/kg, available phosphorus content are 54.7mg/kg, effective potassium content is 383.5mg/kg, the content of organic matter is 4.16g/kg, Water soluble salt total ion concentration is 15.9g/kg, Cl-Content is 5.76g/kg.1: 10 and 1: 20 is obtained after one times of dilution and twice respectively Soil extraction;(5) the soil extraction concentration poured at first is 1: 20, and need to be irrigated during first time nutrient solution, so Poured once every 2~3 days afterwards, each irrigation amount is 250mL, while adding the NH of 1mol/L in leaching liquor4H2PO4Solution 0.5mL, is changed to the soil extraction of pouring 1: 10 after 15 days, after equally pouring 15 days, then the soil extract for being changed to pouring 1: 5 Liquid, after pouring 30 days, transplants when the number of blade is 6~9 leaves;(6) domestication illumination box design temperature is 25 DEG C, and illumination is strong It is 12h/d to spend;(7) hole tray, when outside air temperature reaches 15 DEG C, is moved to outdoor by appropriate hardening together with shallow mouth pallet daily 3~6h of hardening.In May 4, after local nighttime temperature is higher than 15 DEG C, inflexible Qi seedling is transplanted to Jiangsu together with matrix big Feng Jinhai farms salt content pours root water on 2 ‰~4 ‰ salinized soil, and Transplanting Density is 6000 plants every mu.Above-mentioned side The implementation of method can make survival rate of the matrimony vine seedling on the salinized soil that salt content is 2 ‰~4 ‰ improve 11.4%~16.3%.
Embodiment 2
(1) matrimony vine seedling medium peat, vermiculite, perlite, straw ash are well mixed by 3: 3: 3: 1 volume ratio, warp 121 DEG C~124 DEG C high-temperature sterilizations of autoclave are processed 30 minutes, and uniformly insert plastics cave dish;Wherein main component in straw ash P2O5Content is 0.39%, K2O content is 2.84%;(2) plastics cave dish for using, cave number 5 × 10, disk body 54cm long is wide 28cm, cave depth 5cm;Hole tray transfers one shallow mouthful of pallet, in case nutrient solution is lost in;(3) matrimony vine seed first through 75% alcohol disinfecting 2~ After 3 minutes with running water rinse 8 times, then with 2%NaClO solution disinfections 5~6 minutes and with running water flushing 8 times after, dry; Matrix is first inserted in hole tray during sowing, when being substantially flush with hole tray mouth, is flattened, every bunch planting kind 5~6, then tile one layer 0.3cm thick matrix lid crosses seed, is put into culture in incubator.The thinning of neat after seedling once, 2 plants of matrimony vine seedling is stayed per cave;(4) soak Drawings soil is the beach salty soil from jiangsu coast collection.1: 5 vibration extraction 24 is compared with Tu Shui in 25 DEG C of constant temperature oscillators After hour, pumping filtering obtains the soil extraction that Tu Shui compares 1: 5.Soil extraction pH is that 7.55, alkaline hydrolysis nitrogen content is 38.2mg/kg, available phosphorus content are 50.1mg/kg, effective potassium content is 376.9mg/kg, the content of organic matter is 3.98g/kg, Water soluble salt total ion concentration is 14.7g/kg, Cl-Content is 5.13g/kg.1: 10 and 1: 20 is obtained after one times of dilution and twice respectively Soil extraction;(5) the soil extraction concentration poured at first is 1: 20, and need to be irrigated during first time nutrient solution, so Poured once every 2~3 days afterwards, each irrigation amount is 250mL, while adding the NH of 1mol/L in leaching liquor4H2PO4Solution 0.5mL, is changed to the soil extraction of pouring 1: 10 after 15 days, after equally pouring 15 days, then the soil extract for being changed to pouring 1: 5 Liquid, after pouring 30 days, transplants when the number of blade is 6~9 leaves;(6) domestication illumination box design temperature is 25 DEG C, and illumination is strong It is 12h/d to spend;(7) hole tray, when outside air temperature reaches 15 DEG C, is moved to outdoor by appropriate hardening together with shallow mouth pallet daily 3~6h of hardening.In May 6, after local nighttime temperature is higher than 15 DEG C, matrimony vine seedling is transplanted to Jiangsu together with matrix and is penetrated The suitable Thailand's farm salt content of sun pours root water on 4 ‰~6 ‰ salinized soil, and Transplanting Density is 6000 plants every mu.Above-mentioned side The implementation of method can make survival rate of the matrimony vine seedling on the salinized soil that salt content is 4 ‰~6 ‰ improve 8.1%~12.5%.

Claims (6)

1. a kind of matrimony vine seedling medium, it is characterised in that the matrimony vine seedling medium is by peat, vermiculite, perlite, straw ash Mix at 3: 3: 3: 1 by volume.
2. a kind of matrimony vine seedling medium as claimed in claim 1, it is characterised in that the straw ash is biomass power plants generating electricity The main component P of the stalk ash content for producing afterwards, wherein straw ash2O5Content is 0.30%~0.39%, K2O content is 2.75% ~2.84%.
3. a kind of method of utilization matrix culture and improvement matrimony vine seedling salt tolerance, it is characterised in that the method will using such as right The matrimony vine seedling medium described in 1 is sought, pouring nutrient solution stoste is the beach salty soil to be gathered from jiangsu coast, in 25 DEG C of perseverances After comparing 1: 5 vibration extraction 24 hours with Tu Shui in warm oscillator, the soil extraction that pumping filtering is obtained;The soil extraction PH for 7.55~7.78, alkaline hydrolysis nitrogen content be 38.2mg/kg~41.5mg/kg, available phosphorus content be 50.1mg/kg~ 54.7mg/kg, effective potassium content are 376.9mg/kg~383.5mg/kg, the content of organic matter is 3.98g/kg~4.16g/kg, Water soluble salt total ion concentration is 14.7g/kg~15.9g/kg, and Cl contents are 5.13g/kg~5.76g/kg.
4. a kind of method of utilization matrix culture and improvement matrimony vine seedling salt tolerance, it is characterised in that carried out by the steps:
(1) first by 3: 3: 3: 1 mixing by volume of peat, vermiculite, perlite, straw ash, through 121 DEG C~124 DEG C high-temperature sterilizations Treatment uniformly inserts plastics cave dish after 30 minutes;
(2) beach salty soil that will be gathered from jiangsu coast again compares 1: 5 vibration extraction 24 in 25 DEG C of constant temperature oscillators with Tu Shui After hour, pumping filtering obtains the soil extraction that Tu Shui compares 1: 5;1: 10 and 1: 20 soil is obtained after one times of dilution and twice respectively Earth leaching liquor;
(3) after matrimony vine seed is sown into hole tray, the soil extraction that concentration is for 1: 20 is first poured, and need to be irrigated when pouring for the first time, Then poured once every 2~3 days, often each irrigation amount of disk is 250mL, while adding the NH of 1mol/L4H2PO4Solution 0.5mL, is changed to the soil extraction of pouring 1: 10 after 15 days, after equally pouring 15 days, then the soil extract for being changed to pouring 1: 5 Liquid, after pouring 30 days, transplants when the number of blade is 6~9 leaves;
(4) when outside air temperature reaches 15 DEG C, hole tray is moved to outdoor 3~6h of hardening, nursery together with shallow mouth pallet daily It is transplanted on the salinized soil that salt content is 2 ‰~6 ‰ together with matrix after 60 days, and pours water.
5. the method for utilization matrix culture and improvement matrimony vine seedling salt tolerance according to claim 4, it is characterised in that described Plastics cave dish cave number 5 × 10, disk body 54cm long, 28cm wide, cave depth 5cm;Hole tray transfers one shallow mouthful of pallet, in case nutrient solution stream Lose.
6. the method for utilization matrix culture and improvement matrimony vine seedling salt tolerance according to claim 4, it is characterised in that matrimony vine Seed is first rinsed 8 times through after 75% alcohol disinfecting 2~3 minutes with running water, then with 2%NaClO solution disinfections 5~6 minutes simultaneously After rinsing 8 times with running water, dry;Matrix is first inserted in hole tray during sowing, when being substantially flush with hole tray mouth, is flattened, per cave Sowing 5~6, then the one layer of 0.3cm thick matrix lid that tiles crosses seed, and culture in incubator is put into after pouring nutrient solution.Qi Miao Thinning afterwards once, 2 plants of matrimony vine seedling is stayed per cave.
CN201611093466.6A 2016-11-23 2016-11-23 Method for improving salt tolerance of medlar seedlings by utilizing matrix culture Active CN106818432B (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110122302A (en) * 2019-06-04 2019-08-16 青海省畜牧兽医科学院 A kind of oat seedlings breeding method
CN111264369A (en) * 2020-03-17 2020-06-12 甘肃省治沙研究所 Plug seedling strengthening method for lycium ruthenicum murr
CN112449978A (en) * 2020-12-16 2021-03-09 湖南科技大学 Propagation method of salt-tolerant Actinidia valvata Dunn seedlings

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CN101176407A (en) * 2007-04-14 2008-05-14 冯立田 Artificial cultivation technique for black fruit medlar in alkaline land
CN104756696A (en) * 2015-03-24 2015-07-08 河北省林业科学研究院 Breeding method for culturing salt-tolerant plants
CN105052521A (en) * 2015-09-01 2015-11-18 福建省春天园林工程有限公司 Seedling cultivating method for improving salt-resistance performance of pittosporum tobira
CN105325147A (en) * 2015-10-30 2016-02-17 山西火品生物科技有限公司 Method for cultivating and planting lycium ruthenicum murray
CN106116800A (en) * 2016-06-17 2016-11-16 王益祥 A kind of bamboo willow culture fluid and preparation method thereof and bamboo willow inoculation method

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101176407A (en) * 2007-04-14 2008-05-14 冯立田 Artificial cultivation technique for black fruit medlar in alkaline land
CN104756696A (en) * 2015-03-24 2015-07-08 河北省林业科学研究院 Breeding method for culturing salt-tolerant plants
CN105052521A (en) * 2015-09-01 2015-11-18 福建省春天园林工程有限公司 Seedling cultivating method for improving salt-resistance performance of pittosporum tobira
CN105325147A (en) * 2015-10-30 2016-02-17 山西火品生物科技有限公司 Method for cultivating and planting lycium ruthenicum murray
CN106116800A (en) * 2016-06-17 2016-11-16 王益祥 A kind of bamboo willow culture fluid and preparation method thereof and bamboo willow inoculation method

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110122302A (en) * 2019-06-04 2019-08-16 青海省畜牧兽医科学院 A kind of oat seedlings breeding method
CN111264369A (en) * 2020-03-17 2020-06-12 甘肃省治沙研究所 Plug seedling strengthening method for lycium ruthenicum murr
CN112449978A (en) * 2020-12-16 2021-03-09 湖南科技大学 Propagation method of salt-tolerant Actinidia valvata Dunn seedlings

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