CN106116800A - A kind of bamboo willow culture fluid and preparation method thereof and bamboo willow inoculation method - Google Patents

A kind of bamboo willow culture fluid and preparation method thereof and bamboo willow inoculation method Download PDF

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CN106116800A
CN106116800A CN201610439529.2A CN201610439529A CN106116800A CN 106116800 A CN106116800 A CN 106116800A CN 201610439529 A CN201610439529 A CN 201610439529A CN 106116800 A CN106116800 A CN 106116800A
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culture fluid
bamboo willow
aqueous solution
concentration
nursery
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CN106116800B (en
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王益祥
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B7/00Fertilisers based essentially on alkali or ammonium orthophosphates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C21/00Methods of fertilising, sowing or planting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005Cultivation methods
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/20Liquid fertilisers

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Environmental Sciences (AREA)
  • Soil Sciences (AREA)
  • Botany (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention provides a kind of bamboo willow culture fluid and preparation method thereof and the method for bamboo willow cultivation.Described culture fluid is by adding root-growing agent and potassium dihydrogen phosphate such that it is able to promote that bamboo willow takes root and grows;Meanwhile, by adding zostera marina extracting solution in described culture fluid such that it is able to improve the salt tolerance being cultivated the bamboo willow obtained.Described culture fluid has rich in nutrition content, and can improve the advantages such as bamboo willow salt tolerance and survival rate.Meanwhile, present invention also offers the preparation method of a kind of described bamboo willow culture fluid, the method, by preparing bamboo willow culture fluid after each component raw material being mixed, has operational approach easy, the advantages such as obtained culture fluid is effective.Further, present invention also offers the breeding method of a kind of bamboo willow, in the inventive method, the application of the invention culture fluid such that it is able to improve salt tolerance and the survival rate cultivating the bamboo willow obtained.

Description

A kind of bamboo willow culture fluid and preparation method thereof and bamboo willow inoculation method
Technical field
The present invention relates to domestication of plants field, in particular to a kind of bamboo willow culture fluid and preparation method thereof and bamboo willow Inoculation method.
Background technology
In coastal area, due to the fluctuation of sea water, can be formed during the Yancheng Region of large-area beach, such as China just has The Yancheng Seashore that state's coastal area area is maximum.Although the area of beach is very big, but owing to soil property is soft, so port should not be built Mouthful.Simultaneously as the salinity of beach is the highest, so and be not suitable for the growth of general plant, only small part has salt tolerance Straw or like vegetable can naturally grow up, thus at all cannot large-area plantation trees, also cannot realize the transformation to beach.And These all cause the waste of a large amount of land resource of beach.
In order to reasonably utilize Tidal Flat Resources, people establish a lot of new reclamation area at Coastal beach, and have employed implant By, drought modify-water, levee-surrounded field a series of ameliorative measures such as breed fish, it is desirable to be able to increase the soil body salt leaching time, and promote topsoil Earth nutrient accumulation, thus realize the improvement to beach area strongly salined soil.
But, the existing method limited use to the soil improvement of coastal saline soil, and need to put into substantial amounts of manpower, Material resources, and uneconomical;Meanwhile, limited region also can only be carried out by existing modification method, and cannot realize on a large scale Beach carries out the improvement of edaphic condition on the whole.
Planting plants is the improvement very effective method of soil, thus has the bamboo willow etc. of good salt tolerance at growing plants on beach Trees, have the most just become the important means improved beach height saline soil day by day at present.But, owing to the salt of beach is spent Height, even thus there is the bamboo willow of certain salt tolerance, its survival rate on beach is the highest.
Thus, how to improve the Salt tolerant plants survival rates on high salinity beach such as bamboo willow, and then utilize planting plants Realize the effectively transformation of beach has the most just been become current technical problem urgently to be resolved hurrily.
In view of this, the special proposition present invention.
Summary of the invention
The first object of the present invention is to provide a kind of bamboo willow culture fluid, in described culture fluid by add root-growing agent with And potassium dihydrogen phosphate such that it is able to promote that bamboo willow takes root and grows;Meanwhile, by adding zostera marina extracting solution in described culture fluid, It is thus possible to improve the salt tolerance being cultivated the bamboo willow obtained, and then it is poor to solve bamboo willow salt tolerance in prior art, and bamboo willow is on beach It is coated with the technical problems such as survival rate is low.Culture fluid of the present invention has rich in nutrition content, and can improve bamboo willow salt tolerance and survival The advantages such as rate.
The second object of the present invention is to provide the preparation method of a kind of described bamboo willow culture fluid, by by each in the method Prepare bamboo willow culture fluid after component raw material mixing, there is operational approach easy, the advantages such as obtained culture fluid is effective.
Third object of the present invention is to provide the breeding method of a kind of bamboo willow, in the inventive method, by using this Invention culture fluid such that it is able to improve salt tolerance and the survival rate cultivating the bamboo willow obtained.
In order to realize the above-mentioned purpose of the present invention, spy by the following technical solutions:
A kind of bamboo willow culture fluid, according to percent by weight, described culture fluid is mainly made up of following components: root-growing agent 3 ~25%, potassium dihydrogen phosphate 2~25%, seaweed extract 55~90%.
In culture fluid of the present invention, by adding root-growing agent and potassium dihydrogen phosphate such that it is able to promote that bamboo willow takes root and gives birth to Long;Meanwhile, by adding zostera marina extracting solution in described culture fluid such that it is able to improve the salt tolerance being cultivated the bamboo willow obtained, There is rich in nutrition content, and the advantages such as bamboo willow salt tolerance and survival rate can be improved.
In the present invention, described zostera marina is the straw or like vegetable of growth on the beach of seashore.
Optionally, in the present invention, described according to percent by weight, described culture fluid is made up of following components: root-growing agent 3 ~25%, potassium dihydrogen phosphate 2~25%, seaweed extract 55~90%.
Optionally, in the present invention, according to percent by weight, described culture fluid is mainly made up of following components: root-growing agent 5 ~20%, potassium dihydrogen phosphate 5~20%, seaweed extract 65~85%.
In the present invention, by adjusting further and optimizing raw material dosage each in culture fluid, thus further increase The culture effect of culture fluid.
Optionally, in the present invention, according to percent by weight, described culture fluid is made up of following components: root-growing agent 5~ 20%, can be such as but be not limited to 6%, 8%, 10%, 12%, 15%, 16%, 18% or 19% etc.;Potassium dihydrogen phosphate 5~ 20%, can be such as but be not limited to 7%, 10%, 11%, 12%, 13%, 14%, 15%, 17% or 19% etc.;Zostera marina carries Take thing 65~85%, can be such as but be not limited to 66%, 67%, 69%, 70&, 72%, 73%, 75%, 77%, 79%, 80% or 83% etc..
Optionally, in the present invention, described zostera marina extracting solution is in Rhizoma Imperatae, Herba suadeae glaucae, alkali thatch, roe hair, melilotus or phragmites communis The mixed extract of one or more.
In the present invention, by selecting further and optimizing seaweed extract used, thus further increase and trained Educate the salt tolerance of bamboo willow.
Meanwhile, present invention also offers the preparation method of a kind of described culture fluid, described method comprises the steps: life After root agent, potassium dihydrogen phosphate and seaweed extract mixing, and stir, obtain described culture fluid.
In the present invention, by preparing culture fluid after being mixed by each raw material, there is method easy, obtained culture fluid effect The advantage such as good.
Optionally, in the present invention, described method still further comprises after being pulverized by zostera marina, then with alcohol reflux with system The step of standby zostera marina extracting solution.
Optionally, in the present invention, described extraction is addition 80~the ethanol of 95% in the zostera marina after pulverizing, and backflow carries Take 2-5h, then filter, and by filtrate except concentrating after alcohol, obtain described zostera marina extracting solution;Preferably, in the present invention, it is extracted as Add the ethanol of 90-95%, and reflux, extract, 3-4h.
In the present invention, by adjusting further and optimizing extraction conditions such that it is able to by the effective ingredient in zostera marina More fully extract.
Optionally, in the present invention, described reflux, extract, is reflux, extract, 2-4 time;Preferably, in the present invention, described backflow It is extracted as extracting 3 times.
Further, present invention also offers the inoculation method of a kind of bamboo willow, in described method, first bamboo Liu Sui bar is existed Cuttage in substrate, then carries out watering nursery with the aqueous solution of culture fluid of the present invention, and then, seedling cultivation obtained moves Plant and grow to natural beach environment, and obtain bamboo willow.
In the present invention, by bamboo willow is cultivated first by culture fluid of the present invention such that it is able to improve the resistance to of bamboo willow Salt, thus improve its survival rate of growth naturally under the conditions of beach.
In the present invention, described substrate is normal salinity soil matrix, such as black earth, peat etc..
Optionally, in the present invention, described pouring nursery is first to carry out watering nursery with the culture fluid aqueous solution of low concentration, Then the concentration gradually stepping up culture fluid aqueous solution carries out watering nursery.
In the present invention, by being stepped up culture fluid concentration in incubation, so that bamboo Liu Sui bar can In growth course, progressively adapt to culture fluid, and this is also further such that bamboo willow can progressively adapt to high salinity in cultivating process Environment.
Optionally, in the present invention, described pouring nursery be first with concentration be 18~28% culture fluid aqueous solution carry out Pouring nursery, such as can be with cultivation that concentration is 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26% or 27% Liquid aqueous solution carries out watering nursery;Then, then with concentration be 28~32% culture fluid aqueous solution carry out water nursery, such as may be used To cultivate with the culture fluid aqueous solution that concentration is 29%, 30% or 31%;Then, then with cultivation that concentration is 34~37% Liquid aqueous solution carries out watering nursery, such as, can carry out watering nursery with the culture fluid aqueous solution that concentration is 35% or 36%;? After again with concentration be 39~42% culture fluid aqueous solution carry out water nursery, can be such as 40% or 41% with concentration Culture fluid aqueous solution carries out watering nursery;Then seedling establiss cultivation obtained.
In the present invention, by adjustment and the optimization of culture fluid concentration used to stage in incubation, such that it is able to make Bamboo willow in cultivating process normally take root growth while, additionally it is possible to make bamboo willow the most progressively adapt to high salinity nature ring Border.
Optionally, in the present invention, with concentration be 18~28% culture fluid aqueous solution carry out water nursery time for 25 ~30d, the time such as cultivated can be 26,27,38,29,30,31,32,33,34d;With the cultivation that concentration is 28~32% It is 55~65d that liquid aqueous solution carries out watering the time of nursery, the time such as cultivated can be 56,57,58,59,60,61,62, 63、64d;With concentration be 34~37% culture fluid aqueous solution carry out water nursery time be 85~95d, such as, cultivation Time can be 86,87,88,89,90,91,92,93,94d;With concentration be 39~41% culture fluid aqueous solution water The time of nursery is 85~95d, the time such as cultivated can be 86,87,88,89,90,91,92,93,94d.
In the present invention, by culture fluid concentration used interim in incubation and the adjustment of each stage incubation time And optimization so that bamboo willow in cultivating process normally take root growth while, additionally it is possible to make bamboo willow further by Step adapts to high salinity natural environment.
Optionally, in the present invention, with concentration be with concentration be 18~28% culture fluid aqueous solution carry out water nursery 25 ~30d farther includes following steps, first with concentration be 18~20% culture fluid aqueous solution cultivate after 7d;Then with concentration It is the culture fluid aqueous solution cultivation 7d of 21~23%;The most again with concentration be 23~25% culture fluid aqueous solution cultivate 7d;? After again with concentration be 25~28% culture fluid aqueous solution cultivate 7~9d.
Compared with prior art, the invention have the benefit that
(1) in culture fluid of the present invention, by adding root-growing agent, potassium dihydrogen phosphate and zostera marina extracting solution such that it is able to carry While making bamboo willow normal growth for enough nutrient substance, additionally it is possible to improve the salt tolerance being cultivated the bamboo willow obtained;
(2) by zostera marina being extracted with use ethanol in the inventive method, one-step optimization extraction process of going forward side by side, thus Effective ingredient in zostera marina fully can be extracted;
(3) in cultural method of the present invention, by culture fluid concentration used in cultivating and the adjustment of incubation time and excellent Change such that it is able to improve further and cultivate the salt tolerance obtaining bamboo willow and the survival rate grown under the conditions of natural beach.
Detailed description of the invention
Below in conjunction with embodiment, embodiment of the present invention are described in detail, but those skilled in the art will Understanding, the following example is merely to illustrate the present invention, and is not construed as limiting the scope of the present invention.In embodiment unreceipted specifically Condition person, the condition advised according to normal condition or manufacturer is carried out.Agents useful for same or instrument unreceipted production firm person, be Can be by the commercially available conventional products bought and obtain.
Embodiment 1
Weigh appropriate Rhizoma Imperatae, Herba suadeae glaucae, alkali thatch, roe hair, melilotus and phragmites communis respectively, then pulverize, and after pulverizing Raw material adds the ethanol that appropriate concentration is 95%, extracts 4h at reflux, and repeat to extract 3 times.Then, after extracting Mixed system filter, and filtrate is added evanescence of heat alcohol, concentrates the seaweed extract i.e. obtaining embodiment 1.
According to percetage by weight, 15% root-growing agent, 15% potassium dihydrogen phosphate and 70% seaweed extract, weigh suitable respectively Measure each raw material, then mix homogeneously, be designated as embodiment 1 bamboo willow culture fluid.
It is selected to the bamboo willow female parent that long strong and without pest and disease damage 1-2 grows, and chooses a length of 8-10cm shoot, so After in the morning by shoot shear after disinfect, then will shear after shoot on peat after cuttage water nursery, specifically Step is as follows:
(1) configuration concentration is the culture fluid aqueous solution of embodiment 1 of 20%, then waters shoot after cuttage, often Day pouring 3 times, and persistently water 30d;
(2), after pouring 30d, configuration concentration is the culture fluid aqueous solution of the embodiment 1 of 30%, after then proceeding to cuttage Shoot is watered, and waters every day 3 times, and again waters 60d;
(3), after again watering 60d, configuration concentration is the culture fluid aqueous solution of the embodiment 1 of 35%, then proceedes to cuttage After shoot water, water every day 3 times, and continue water 90d;
(4) after continuing pouring 90d, configuration concentration is the culture fluid aqueous solution of the embodiment 1 of 40%, then proceedes to skewer Shoot after Chaing is watered, and waters every day 3 times, and waters 90d, i.e. obtains the bamboo willow Seedling of embodiment 1.
Embodiment 2
Prepare culture fluid according to the method for embodiment 1, be designated as embodiment 2 bamboo willow culture fluid.
It is selected to the bamboo willow female parent that long strong and without pest and disease damage 1-2 grows, and chooses a length of 8-10cm shoot, so After in the morning by shoot shear after disinfect, then will shear after shoot on peat after cuttage water nursery, specifically Step is as follows:
(1) configuration concentration is the culture fluid aqueous solution of embodiment 2 of 20%, then waters shoot after cuttage, often Day pouring 3 times, and persistently water 7d;
(2) after persistently watering 7d, configuration concentration is the culture fluid aqueous solution of the embodiment 2 of 22%, and continues to water 7d, often Day pouring 3 times;
(3), after continuing pouring 7d, configuration concentration is the culture fluid aqueous solution of the embodiment 2 of 25%, and again waters 7d, often Day pouring 3 times;
(4), after again watering 7d, configuration concentration is the culture fluid aqueous solution of the embodiment 2 of 28%, and continues sebific duct 7d, often Day pouring 3 times;
(5), after continuing pouring 7d, configuration concentration is the culture fluid aqueous solution of the embodiment 2 of 30%, then proceedes to cuttage After shoot water, water every day 3 times, and again water 60d;
(6), after again watering 60d, configuration concentration is the culture fluid aqueous solution of the embodiment 2 of 35%, then proceedes to cuttage After shoot water, water every day 3 times, and continue water 90d;
(7) after continuing pouring 90d, configuration concentration is the culture fluid aqueous solution of the embodiment 2 of 40%, then proceedes to skewer Shoot after Chaing is watered, and waters every day 3 times, and waters 90d, i.e. obtains the bamboo willow Seedling of embodiment 2.
Embodiment 3
Seaweed extract is prepared according to method described in embodiment 1, then according to percetage by weight, 12% root-growing agent, 18% Potassium dihydrogen phosphate and 70% seaweed extract, weigh appropriate each raw material, then mix homogeneously respectively, is designated as embodiment 3 bamboo willow Culture fluid.
Then, it is selected to the bamboo willow female parent that long strong and without pest and disease damage 1-2 grows, and it is new to choose a length of 8-10cm Branch, disinfects after being sheared by shoot the most in the morning, and the shoot after then shearing waters nursery on peat after cuttage, Specifically comprise the following steps that
(1) configuration concentration is the culture fluid aqueous solution of embodiment 3 of 20%, then waters shoot after cuttage, often Day pouring 3 times, and persistently water 30d;
(2), after pouring 30d, configuration concentration is the culture fluid aqueous solution of the embodiment 3 of 30%, after then proceeding to cuttage Shoot is watered, and waters every day 3 times, and again waters 60d;
(3), after again watering 60d, configuration concentration is the culture fluid aqueous solution of the embodiment 3 of 35%, then proceedes to cuttage After shoot water, water every day 3 times, and continue water 90d;
(4) after continuing pouring 90d, configuration concentration is the culture fluid aqueous solution of the embodiment 3 of 40%, then proceedes to skewer Shoot after Chaing is watered, and waters every day 3 times, and waters 90d, i.e. obtains the bamboo willow Seedling of embodiment 3.
Embodiment 4
Prepare culture fluid according to the method described in embodiment 3, be designated as embodiment 4 bamboo willow culture fluid.
Then, it is selected to the bamboo willow female parent that long strong and without pest and disease damage 1-2 grows, and it is new to choose a length of 8-10cm Branch, disinfects after being sheared by shoot the most in the morning, and the shoot after then shearing waters nursery on peat after cuttage, Specifically comprise the following steps that
(1) configuration concentration is the culture fluid aqueous solution of embodiment 4 of 20%, then waters shoot after cuttage, often Day pouring 3 times, and persistently water 7d;
(2) after persistently watering 7d, configuration concentration is the culture fluid aqueous solution of the embodiment 4 of 22%, and continues to water 7d, often Day pouring 3 times;
(3), after continuing pouring 7d, configuration concentration is the culture fluid aqueous solution of the embodiment 4 of 25%, and again waters 7d, often Day pouring 3 times;
(4), after again watering 7d, configuration concentration is the culture fluid aqueous solution of the embodiment 4 of 28%, and continues sebific duct 7d, often Day pouring 3 times;
(5), after continuing pouring 7d, configuration concentration is the culture fluid aqueous solution of the embodiment 4 of 30%, then proceedes to cuttage After shoot water, water every day 3 times, and again water 60d;
(6), after again watering 60d, configuration concentration is the culture fluid aqueous solution of the embodiment 4 of 35%, then proceedes to cuttage After shoot water, water every day 3 times, and continue water 90d;
(7) after continuing pouring 90d, configuration concentration is the culture fluid aqueous solution of the embodiment 4 of 40%, then proceedes to skewer Shoot after Chaing is watered, and waters every day 3 times, and waters 90d, i.e. obtains the bamboo willow Seedling of embodiment 4.
Embodiment 5
Seaweed extract is prepared according to method described in embodiment 1, then according to percetage by weight, 5% root-growing agent, 5% phosphorus Acid dihydride potassium and 90% seaweed extract, weigh appropriate each raw material, then mix homogeneously respectively, is designated as embodiment 5 bamboo Liu Pei Nutrient solution.
Then, with the culture fluid of embodiment 5 as culture fluid, and grow saplings according to step described in embodiment 2, i.e. obtain reality Execute the bamboo willow Seedling of example 5.
Embodiment 6
Seaweed extract is prepared according to method described in embodiment 1, then according to percetage by weight, 23% root-growing agent, 22% Potassium dihydrogen phosphate and 55% seaweed extract, weigh appropriate each raw material, then mix homogeneously respectively, is designated as embodiment 6 bamboo willow Culture fluid.
Then, with the culture fluid of embodiment 6 as culture fluid, and grow saplings according to step described in embodiment 2, i.e. obtain reality Execute the bamboo willow Seedling of example 6.
Comparative example 1
According to percetage by weight, 40% root-growing agent and 60% potassium dihydrogen phosphate, weigh appropriate each raw material respectively, then mix Close uniformly, be designated as comparative example 1 bamboo willow culture fluid.
Then, it is culture fluid according to the culture fluid of comparative example 1, and grows saplings according to step described in embodiment 1, i.e. obtain The bamboo willow Seedling of comparative example 1.
Comparative example 2
Seaweed extract is prepared according to method described in embodiment 1, then according to percetage by weight, 20% root-growing agent, 45% Potassium dihydrogen phosphate and seaweed extract 35%, weigh appropriate each raw material, then mix homogeneously respectively, is designated as comparative example 2 bamboo willow Culture fluid.
Then, it is culture fluid according to the culture fluid of comparative example 2, and grows saplings according to step described in embodiment 1, i.e. obtain The bamboo willow Seedling of comparative example 2.
Comparative example 3
Seaweed extract is prepared according to method described in embodiment 1, then according to percetage by weight, 2% root-growing agent, 3% phosphorus Acid dihydride potassium and seaweed extract 95%, weigh appropriate each raw material, then mix homogeneously respectively, is designated as comparative example 3 bamboo Liu Pei Nutrient solution.
Then, it is culture fluid according to the culture fluid of comparative example 3, and grows saplings according to step described in embodiment 1, i.e. obtain The bamboo willow Seedling of comparative example 3.
Effect example 1
Choose 100 strain embodiments 1-6 and the normal growth of comparative example 1-3 respectively and highly for the bamboo willow of about 6m Seedling, then in the case of not damage tree shoot root palpus, by seedling band soil from substrate take out, and ensure seedling band soil 30cm with On.
Band soil seedling is transferred to Yancheng City Dongtai City Coastal beach that mean salt amount is 2.58g/Kg, and each group is set Seedling is planted in closing on beach respectively, and planting density is 500 Seedlings/mu.Then, allow seedling grow under field conditions (factors), then exist After 360d, each group of seedling survival condition carrying out record statistics, and calculates seedling survival rate, result is as follows:
Group Survival rate (× 100%)
Embodiment 1 83
Embodiment 2 92
Embodiment 3 83
Embodiment 4 94
Embodiment 5 75
Embodiment 6 78
Comparative example 1 35
Comparative example 2 61
Comparative example 3 70
Then, the salinity to each group of bamboo willow kind region beach is sampled detection, and each region of statistical computation the most again Mean salt amount, result is as follows:
Region group Salinity (g/Kg)
Embodiment 1 1.73
Embodiment 2 1.51
Embodiment 3 1.78
Embodiment 4 1.43
Embodiment 5 1.89
Embodiment 6 1.92
Comparative example 1 2.11
Comparative example 2 1.99
Comparative example 3 2.01
Culture fluid of the present invention can improve the salt tolerance of cultivated bamboo willow, and improves depositing of under the conditions of natural beach bamboo willow Motility rate, thus contribute to beach is carried out soil improvement.
Although illustrate and describing the present invention with specific embodiment, but it will be appreciated that without departing substantially from the present invention's May be made that in the case of spirit and scope many other change and amendment.It is, therefore, intended that in the following claims Including all such changes and modifications belonged in the scope of the invention.

Claims (10)

1. a bamboo willow culture fluid, it is characterised in that according to percent by weight, described culture fluid is mainly by following components system Become: root-growing agent 3~25%, potassium dihydrogen phosphate 2~25%, seaweed extract 55~90%.
Culture fluid the most according to claim 1, it is characterised in that according to percent by weight, described culture fluid mainly by Following components is made: root-growing agent 5~20%, potassium dihydrogen phosphate 5~20%, seaweed extract 65~85%.
Culture fluid the most according to claim 1 and 2, it is characterised in that described zostera marina extracting solution be Rhizoma Imperatae, Herba suadeae glaucae, alkali thatch, The mixed extract of one or more in roe hair, melilotus or phragmites communis.
4. the preparation method of culture fluid according to any one of claim 1-3, it is characterised in that described method includes walking as follows Rapid: after root-growing agent, potassium dihydrogen phosphate and seaweed extract are mixed, and to stir, obtain described culture fluid.
Method the most according to claim 4, it is characterised in that described method still further comprises after being pulverized by zostera marina, then With alcohol reflux to prepare the step of zostera marina extracting solution.
Method the most according to claim 5, it is characterised in that described extraction be to pulverize after zostera marina in add 80~ The ethanol of 95%, and reflux, extract, 2-5h, then filter, and by filtrate except concentrating after alcohol, obtains described zostera marina extracting solution.
7. the inoculation method of a bamboo willow, it is characterised in that first by bamboo Liu Sui bar cuttage in substrate, then with claim The aqueous solution of culture fluid according to any one of 1-3 carries out watering nursery, and then, seedling cultivation obtained migrates to nature beach Environment grows, and obtains bamboo willow.
Inoculation method the most according to claim 7, it is characterised in that described pouring nursery is first cultivation with low concentration Liquid aqueous solution carries out watering nursery, and the concentration then gradually stepping up culture fluid aqueous solution carries out watering nursery.
Inoculation method the most according to claim 8, it is characterised in that described pouring nursery be first with concentration be 18~ The culture fluid aqueous solution of 28% carries out watering nursery;Then, then with concentration be 28~32% culture fluid aqueous solution water Nursery;Then, then with concentration be 34~37% culture fluid aqueous solution carry out water nursery;Last is 39~42% with concentration the most again Culture fluid aqueous solution carry out water nursery, the seedling establiss then cultivation obtained.
Inoculation method the most according to claim 9, it is characterised in that with the culture fluid aqueous solution that concentration is 18~22% The time carrying out watering nursery is 25~30d;With concentration be 26~32% culture fluid aqueous solution carry out water nursery time It is 55~65d;With concentration be 34~37% culture fluid aqueous solution carry out water nursery time be 85~95d;With concentration it is 39~41% culture fluid aqueous solution carry out water nursery time be 85~95d.
CN201610439529.2A 2016-06-17 2016-06-17 A kind of bamboo willow culture solution and preparation method thereof and bamboo willow inoculation method Expired - Fee Related CN106116800B (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106818432A (en) * 2016-11-23 2017-06-13 江苏沿海地区农业科学研究所 A kind of method of utilization matrix culture and improvement matrimony vine seedling salt tolerance
CN107173147A (en) * 2017-05-23 2017-09-19 黑龙江省润成新能源科技发展有限公司 The cultural method of severe cold areas beneficial bacteria willow
CN110144226A (en) * 2019-06-13 2019-08-20 生态环境部南京环境科学研究所 A kind of cogongrass growth-promoting media and preparation method thereof applied to mining soil reparation
CN111316827A (en) * 2020-03-23 2020-06-23 新疆中泰纺织集团有限公司 Bamboo willow planting method

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1289743A (en) * 2000-11-17 2001-04-04 解俊峰 Composite organic liquid fertilizer
CN101779586A (en) * 2010-02-06 2010-07-21 东营市农业科学研究所 Technique for forestation of Chinese tamarisk on coastal tidal flats
JP2011211935A (en) * 2010-03-31 2011-10-27 Jnc Co Ltd Alginic acid-decomposing treatment agent, and method for producing the same

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1289743A (en) * 2000-11-17 2001-04-04 解俊峰 Composite organic liquid fertilizer
CN101779586A (en) * 2010-02-06 2010-07-21 东营市农业科学研究所 Technique for forestation of Chinese tamarisk on coastal tidal flats
JP2011211935A (en) * 2010-03-31 2011-10-27 Jnc Co Ltd Alginic acid-decomposing treatment agent, and method for producing the same

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106818432A (en) * 2016-11-23 2017-06-13 江苏沿海地区农业科学研究所 A kind of method of utilization matrix culture and improvement matrimony vine seedling salt tolerance
CN107173147A (en) * 2017-05-23 2017-09-19 黑龙江省润成新能源科技发展有限公司 The cultural method of severe cold areas beneficial bacteria willow
CN110144226A (en) * 2019-06-13 2019-08-20 生态环境部南京环境科学研究所 A kind of cogongrass growth-promoting media and preparation method thereof applied to mining soil reparation
CN110144226B (en) * 2019-06-13 2021-04-09 生态环境部南京环境科学研究所 Festuca arundinacea growth solution applied to mining area soil remediation and preparation method thereof
CN111316827A (en) * 2020-03-23 2020-06-23 新疆中泰纺织集团有限公司 Bamboo willow planting method

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