AU2020213315A1 - Methods for seedling culture by direct seeding of cremastra appendiculata seeds - Google Patents
Methods for seedling culture by direct seeding of cremastra appendiculata seeds Download PDFInfo
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- AU2020213315A1 AU2020213315A1 AU2020213315A AU2020213315A AU2020213315A1 AU 2020213315 A1 AU2020213315 A1 AU 2020213315A1 AU 2020213315 A AU2020213315 A AU 2020213315A AU 2020213315 A AU2020213315 A AU 2020213315A AU 2020213315 A1 AU2020213315 A1 AU 2020213315A1
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- AU
- Australia
- Prior art keywords
- seeds
- seedbed
- seedlings
- leaves
- cremastra appendiculata
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
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- 238000000034 method Methods 0.000 title claims abstract description 25
- 241000846172 Cremastra appendiculata Species 0.000 title claims abstract description 22
- 238000010899 nucleation Methods 0.000 title claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 19
- 229930191978 Gibberellin Natural products 0.000 claims abstract description 10
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000003448 gibberellin Substances 0.000 claims abstract description 10
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 8
- 239000002689 soil Substances 0.000 claims abstract description 5
- 230000010152 pollination Effects 0.000 claims abstract description 4
- 230000004083 survival effect Effects 0.000 abstract description 5
- 238000004904 shortening Methods 0.000 abstract description 2
- 230000035784 germination Effects 0.000 description 3
- 241000846171 Cremastra Species 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 230000007226 seed germination Effects 0.000 description 2
- 239000008399 tap water Substances 0.000 description 2
- 235000020679 tap water Nutrition 0.000 description 2
- 241000233866 Fungi Species 0.000 description 1
- VAYOSLLFUXYJDT-RDTXWAMCSA-N Lysergic acid diethylamide Chemical compound C1=CC(C=2[C@H](N(C)C[C@@H](C=2)C(=O)N(CC)CC)C2)=C3C2=CNC3=C1 VAYOSLLFUXYJDT-RDTXWAMCSA-N 0.000 description 1
- 238000009412 basement excavation Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000007844 bleaching agent Substances 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000005059 dormancy Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- QWPPOHNGKGFGJK-UHFFFAOYSA-N hypochlorous acid Chemical compound ClO QWPPOHNGKGFGJK-UHFFFAOYSA-N 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000007665 sagging Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
Abstract
The invention provides a method for seedling culture by direct seeding of Cremastra
appendiculata seeds, which comprises the following steps: a. placing powdered leaves and
native topsoil in a cultivation box from top to bottom, and then pouring with water to prepare
a seedbed for later use; b. selecting wild unopened mature fruit pods or unopened mature fruit
pods obtained by artificial pollination as spare seeds; c. rinsing the seeds with water after
being treated with gibberellin, and then seeding them on the powdered leaves of the seedbed
and covering them with thin soil, so as to prepare a planting seedbed; and d. placing the
planting seedbed in a ventilated and humid environment until the leaves of the seedlings are
unearthed, and then transplanting the seedlings. The invention has the advantages of
shortening the seedling cultivation time and improving the survival rate of Cremastra
appendiculata.
7
Description
Methods for seedling culture by direct seeding of
Cremastra appendiculata seeds
The invention relates to the technical field of agricultural seedling culture, in particular
to methods for seedling culture by direct seeding of Cremastra appendiculata seeds.
BACKGROUND OF INVENTION Cremastra appendiculata (D. Don) Makino has an ovoid or subglobose conforrmed pseudobulb, which is articulated, and externally torn into a fibrous residual sheath. It has usually one leaf which is grown on top of the pseudobulb, narrowly oval, suboval or oblanceolate oval. Scapes emanate from the upper node of the pseudobulb and stand near upright. Racemes are 10-25 cm long with 5-22 flowers. Flowers are often on one side of the inflorescence, somewhat saggy, not fully open, fragrant, narrowly bell-shaped, and pale purple-brown. Petals are oblanceolate or narrowly lanceolate, narrowed toward the base into a narrow line shape. They are 1.8-2.6 cm long, and 3-3.5 mm wide at the upper part, and have a tapering apex. Lips are nearly equal in length to the petals, linear, 3-cleft at the upper quarter. The capsules are nearly elliptical and sagging. The flowering period is from May to June, and the fruiting period is from September to December. It is grown on a subforest
wetland or a ditch wetland at an elevation of 500-2900m. Cremastra appendiculata is subjected to long-term predatory excavation due to its high medicinal and horticultural value. Moreover, due to the limitation of the self-reproduction mechanism, the cultivation propagation period of Cremastra appendiculata is long, in turn causing the resources of Cremastra appendiculata in danger of being exhausted.
The object of the present invention is to provide a method for seedling culture by direct
seeding of Cremastra appendiculata seeds. The invention has the advantages of shortening the
seedling cultivation time and improving the survival rate of Cremastra appendiculata.
The technical solution of the invention lies in a method for seedling culture by direct seeding of Cremastra appendiculata seeds, which comprises the following steps:
a. placing powdered leaves and native topsoil in a cultivation box from top to bottom,
and then pouring with water to prepare a seedbed for later use;
b. selecting wild unopened mature fruit pods or unopened mature fruit pods obtained by
artificial pollination as spare seeds;
c. rinsing the seeds with water after being treated with gibberellin, and then seeding
them on the powdered leaves of the seedbed and covering them with thin soil, so as to
prepare a planting seedbed;
d. placing the planting seedbed in a ventilated and humid environment until the leaves
of the seedlings are unearthed, and then transplanting the seedlings.
In the step a of the above-described method for seedling culture by direct seeding of
Cremastra appendiculata seeds, the total thickness of the powdered leaves and native topsoil
in the cultivation box is 3 to 5 cm.
In the step a of the above-described method for seedling culture by direct seeding of
Cremastra appendiculata seeds, the water poured through the bed is spring water; and in the
step c, the water for rinsing the seeds is spring water.
In the step d of the above-described method for seedling culture by direct seeding of
Cremastra appendiculata seeds, the temperature and the humidity of the ventilated and humid
environment in which the seedbed is placed are 16 to 28°C, and > 90%, respectively.
In the step d of the above-described method for seedling culture by direct seeding of
Cremastra appendiculata seeds, the seedlings can be transplanted when their leaves are
unearthed at a height of 5 cm.
In the step c of the above-described method for seedling culture by direct seeding of
Cremastra appendiculata seeds, gibberellin treatment of the seeds particularly relates to
treatment with 5% gibberellin solution for 1 to 2 hours.
Beneficial effect
Compared to the prior art, the method of the present invention can obtain a large
number of robust seedlings by direct seeding and fine management of Cremastra
appendiculata seeds. Compared to methods for tissue culture and rapid propagation of plants,
the culture conditions for the method of the present invention are simple, and the seedling
culture costs are reduced. The survival rate of transplanted seedlings for the method of the
present invention is more than 90%, and as for the methods for tissue culture and rapid
propagation of plants, a survival rate being only about 80% is satisfied. Compared to the
segmental propagation, the method of the present invention can obtain a large number of
seedlings, and can meet the requirements of industrialized seedlings. The present invention
provides a new means for the large-scale reproduction of the Cremastra appendiculata, and
effectively solves the problems of the current resource requirement of Cremastra
appendiculata and the shortage of seedling resources.
Examples
The present invention will be further described in connection with the examples, which
are not as a basis for limiting the invention.
Example 1
A method for seedling culture by direct seeding of Cremastra appendiculata seeds was
carried out as follows:
a. placing powdered leaves and native topsoil in a cultivation box from top to bottom,
and then pouring with water to prepare a seedbed for later use. According to the invention,
powdered leaves (specifically, saprophytic leaves) are used to provide an air-permeable and
water-retaining nutrient medium for seed germination. Furthermore, symbiotic fungi for seed
germination of Cremastra appendiculata are contained in the native soil to provide the seeds
with a good germination environment, so that the seeds are easier to germinate to generate
young pseudobulbs, and in turn improve the seedling yield.
b. selecting wild unopened mature fruit pods or unopened mature fruit pods obtained by
artificial pollination as spare seeds. A large number of studies from the applicant have
discovered that the seeds used in the present invention have strong activity, are easy to
germinate, and can effectively improve the yield of subsequent seedlings.
c. rinsing the seeds with water after being treated with gibberellin, and then seeding
them on the powdered leaves of the seedbed and covering them with thin soil, so as to
prepare a planting seedbed. By the treatment with the gibberellin, the dormancy of the seeds
can be broken and the early germination of the seeds can be promoted.
d. placing the planting seedbed in a ventilated and humid environment until the leaves
of the seedlings are unearthed, and then transplanting the seedlings. Using the above method,
the protocorms can be seen in 3 to 4 months, and the emergence of seedling leaves can be
seen in 4 to 5 months, which greatly shortens the time for culturing the seedlings of
Cremastra appendiculata (conventional culture usually takes 1to 2 years, or even it is
extremely difficult to see seedlings for 1 to 2 years).
Specifically, in the aforementioned step a, the total thickness of the powdered leaves
and native topsoil in the cultivation box was 3 to 5 cm. This method not only provided a
shading space for seed germination, but also provides adequate nutrition and moisture
retention.
Specifically, in the aforementioned step a, the water poured through the bed was spring
water; and in the step c, the water for rinsing the seeds was spring water. First, the use of
spring water, which is rich in mineral nutrients, can better promote the growth of seedlings.
Second, the flowing spring water contains less germs to prevent the germs from harming the
seedlings. Third, the use of spring water also avoids the damage of the root system of the
seedlings and prevention of the growth of the seedlings by the bleach sterilizing substances
(i.e., hypochlorous acid) in the tap water when tap water is used.
Specifically, in the aforementioned step d, the temperature and the humidity of the ventilated and humid environment in which the seedbed was placed were 16 to 28 °C, and >
%, respectively. In such a temperature and humidity environment, the growth of the
seedlings can be better promoted, and the seedling growing period can be shortened.
Specifically, in the aforementioned step d, the seedlings could be transplanted when
their leaves are unearthed at a height of 5 cm. The selection of 5 cm high strong seedlings can
effectively improve the survival rate of transplanted seedlings.
Specifically, in the aforementioned step c, gibberellin treatment of the seeds particularly
relates to treatment with 5% gibberellin solution for 1 to 2 hours.
Claims (5)
1. A method for seedling culture by direct seeding of Cremastra appendiculata seeds,
which comprises the following steps:
a. placing powdered leaves and native topsoil ina cultivation box from top to bottom,
and then pouring with water to prepare a seedbed for later use;
b. selecting wild unopened mature fruit pods or unopened mature fruit pods obtained by
artificial pollination as spare seeds;
c. rinsing the seeds with water after being treated with gibberellin, and then seeding
them on the powdered leaves of the seedbed and covering them with thin soil, so as to
prepare a planting seedbed; and
d. placing the planting seedbed in a ventilated and humid environment until the leaves
of the seedlings are unearthed, and then transplanting the seedlings.
2. The method according to claim 1, wherein in the step a, the total thickness of the
powdered leaves and native topsoil in the cultivation box is 3 to 5 cm.
3. The method according to claim 1, wherein in the step d, the temperature and the
humidity of the ventilated and humid environment in which the seedbed is placed are 16 to
28°C, and > 90%, respectively.
4. The method according to claim 1, wherein in the step d, the seedlings can be
transplanted when their leaves are unearthed at a height of 5 cm.
5. The method according to claim 1, wherein in the step c, the seeds are treated with 5%
gibberellin solution for 1 to 2 hours.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910330157.3 | 2019-04-23 | ||
| CN201910330157.3A CN109924097A (en) | 2019-04-23 | 2019-04-23 | A kind of method of Cremastra appendiculata seed sowing nursery |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AU2020213315A1 true AU2020213315A1 (en) | 2020-12-10 |
Family
ID=66990839
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2020213315A Abandoned AU2020213315A1 (en) | 2019-04-23 | 2020-05-21 | Methods for seedling culture by direct seeding of cremastra appendiculata seeds |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN109924097A (en) |
| AU (1) | AU2020213315A1 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110786244A (en) * | 2019-12-12 | 2020-02-14 | 遵义市龙驰生物科技有限公司 | Tissue culture and rapid propagation method for seedlings of azalea |
| CN114467387A (en) * | 2022-01-20 | 2022-05-13 | 镇江常青农林科技有限公司 | Standardized cultivation system of chinese herbal medicine |
| CN114651723A (en) * | 2022-04-06 | 2022-06-24 | 西南林业大学 | Culture medium and method for inducing one-step seedling formation of rhododendron pseudobulb plant |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100444722C (en) * | 2006-01-18 | 2008-12-24 | 贵州省农业科学院生物技术研究所 | Cyrtopterin tissue culturing method and fast reproduction thereof |
| CN106804428B (en) * | 2017-01-24 | 2018-08-24 | 四川农业大学 | A kind of Cremastra appendiculata method for culturing seedlings |
| CN106900316B (en) * | 2017-02-06 | 2020-04-07 | 云南农业大学 | Method for natural propagation of wild large-flower flax grass seeds |
| CN107896796A (en) * | 2017-12-06 | 2018-04-13 | 湖南省雅迭香科技有限公司 | A kind of rosemary seed seedling-raising method |
| CN107996305A (en) * | 2018-01-29 | 2018-05-08 | 明光市大地农业科技有限公司 | A kind of Stevia seed method for culturing seedlings |
-
2019
- 2019-04-23 CN CN201910330157.3A patent/CN109924097A/en active Pending
-
2020
- 2020-05-21 AU AU2020213315A patent/AU2020213315A1/en not_active Abandoned
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110786244A (en) * | 2019-12-12 | 2020-02-14 | 遵义市龙驰生物科技有限公司 | Tissue culture and rapid propagation method for seedlings of azalea |
| CN114467387A (en) * | 2022-01-20 | 2022-05-13 | 镇江常青农林科技有限公司 | Standardized cultivation system of chinese herbal medicine |
| CN114651723A (en) * | 2022-04-06 | 2022-06-24 | 西南林业大学 | Culture medium and method for inducing one-step seedling formation of rhododendron pseudobulb plant |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109924097A (en) | 2019-06-25 |
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