CN106818162A - A kind of indoor culture method of white potato seedling potato seed - Google Patents
A kind of indoor culture method of white potato seedling potato seed Download PDFInfo
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- CN106818162A CN106818162A CN201710035386.3A CN201710035386A CN106818162A CN 106818162 A CN106818162 A CN 106818162A CN 201710035386 A CN201710035386 A CN 201710035386A CN 106818162 A CN106818162 A CN 106818162A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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Abstract
It is a kind of indoor culture method of white potato seedling potato seed the invention discloses the present invention.The true seed that will be harvested then, through abolishing rest treatment, vernalization, carries out true seed sowing culture production seedling seed tuber in culturing room.Mainly include the following steps that：Matrix prepares, seedlings nursing plate prepares, abolishes true seed rest period, vernalization, sowing, management, results.Current year's production goes out the potato that grows directly from seeds after this method harvests true seed then, and more than 1 year is shortened than existing breeding technique, improves breeding efficiency；The small potato that grows directly from seeds is directly produced in seedlings nursing plate, is reduced and is transplanted recruitment, save production of seed stock land used；Directly cultivated in seedlings nursing plate and grown directly from seeds potato, it is to avoid be dead during small transplantation of seedlings and cause breeding gene type to lose and influence breeding results；Avoid the soil pest and disease damage problem that conventional cultivation mode causes；Matrix is drawn materials easily using local natural resources pine needle detritus soil, the nutrition supply plant strain growth being rich in using detritus soil without fertilising, and matrix, low cost.
Description
Technical field
The present invention relates to a kind of indoor culture method of white potato seedling potato seed, belong to potato planting technical field.
Background technology
The current Potato Breeding method of China is mainly by sexual hybridization or selfing acquisition true seed, treats true seed
Not after the eye phase, Pregermination and seedling breeding is transplanted, the New Potato Varieties of seed selection high-quality, high yield from seedling clonal offspring.Ma Ling
In potato conventional breeding, variation appears in first-filial generation, that is, hybrid seedling generation, and variation proterties is solid by stem tuber vegetative propagation
Deciding is handed down from age to age.Because potato Cross fertile rate is low, the probability for obtaining hybridization true seed is small, it is necessary to do substantial amounts of miscellaneous
Hand over work, and a certain hybrid generation turns into the improved seeds only probability of a ten thousandth in combining, and breeding of new variety needs
Huge colony, therefore, after obtaining hybridization true seed, to improve hybridization true seed planting percent, seedling breeding link ten
Divide important.In current breeding process, after hybridizing acquisition true seed, next year cultivates seedling, is cultivated after transplanting and obtained
Seedling seed tuber, it has the disadvantage that seedling can not completely be survived during transplanting and cause some genotype losses, gives breeding work
Bring influence.
Seedling is very thin and delicate, and early development is slow, creates the excellent environment of suitable seedling growth, improves planting percent very
It is crucial.
The technical scheme for being related to Some New True Potato Seed vernalization and nursery and seedling seed tuber to cultivate is few, by inquiring about
Documents and materials find that the method described in these documents has it to act on, but only relate to Some New True Potato Seed vernalization and nursery and
Subproblem in seedling seed tuber cultivation, can not solve all problems of seedling seed tuber cultivation.Therefore Potato Breeding is accelerated
Process, improving Potato Breeding efficiency becomes problem demanding prompt solution.
The content of the invention
It is an object of the present invention to provide a kind of indoor culture method of white potato seedling potato seed.The method can be accelerated
Potato Breeding process, improves Potato Breeding efficiency.
Technical scheme：A kind of indoor culture method of white potato seedling potato seed, the method includes following step
Suddenly：
A, matrix prepare：Pine needle detritus soil mesh is the sieve of 10~15mm, and is made after being disinfected
It is matrix；
B, abolish true seed rest period：Harvested after fruit maturation of growing directly from seeds, be subsequently placed in hole plate seedling growth disk, cover wet hair
Towel, is placed on and allow at room temperature its after-ripening, and checks at any time, and when growing directly from seeds, fruit softens, and is cleaned when gently extruding is i.e. broken, obtains true seed
Afterwards, seed is wrapped with gauze, is put into the gibberellin liquid of 1000-1200ppm and soaks 28-32 hours, breaking dormancy；
D, presprouting of seeds：Vernalization is carried out by the 23-28 DEG C of interior of temperature is placed in by the seed of seed soaking, is used daily
23-28 DEG C of warm water washing once plants bag, and checks Seed sprouting situation, after seed shows money or valuables one carries unintentionally by sow；
E, sowing：Matrix is loaded in seedlings nursing plate hole before sowing, then seed is sowed in hole tray, after planting cover base
Matter, then pour permeable, it is placed on the culturing rack of culturing room；
F, management：Temperature control, after planting to the stage of emerging, it is 13~18 DEG C to keep culture indoor temperature, and seedling stage keeps
Temperature is 17~22 DEG C, and knot potato phase keeping temperature is 13~19 DEG C；Moisture is controlled, and is after planting poured once daily to the stage of emerging
Water, keeps matrix moistening, and plant strain growth pours a water every other day to envelope woods from after emerging, and when blade starts to turn yellow, stops pouring
Water；
G, results：After matrix is dry, plant is lifted, harvest potato seed, the potato seed of same combination is mounted in same sand net bag
It is interior, hang up properly label preservation.
The indoor culture method of foregoing white potato seedling potato seed, the method includes following steps：
A, matrix prepare：Pine needle detritus soil mesh is the sieve of 10~15mm, and is made after being disinfected
It is matrix；
B, abolish true seed rest period：Harvested after fruit maturation of growing directly from seeds, be subsequently placed in hole plate seedling growth disk, cover wet hair
Towel, is placed on and allow at room temperature its after-ripening, and checks at any time, and when growing directly from seeds, fruit softens, and is cleaned when gently extruding is i.e. broken, obtains true seed
Afterwards, seed is wrapped with gauze, is put into the gibberellin liquid of 1100ppm and soaks 30 hours, breaking dormancy；
D, presprouting of seeds：Vernalization is carried out by 26 DEG C of interiors of temperature are placed in by the seed of seed soaking, daily with 26 DEG C
Warm water washing once plant bag, and check Seed sprouting situation, after seed shows money or valuables one carries unintentionally by sow；
E, sowing：Matrix is loaded in seedlings nursing plate hole before sowing, then seed is sowed in hole tray, every bunch planting kind one
Seed, after planting covers matrix, then pours permeable, and matrix lid is neat with card, is placed on the culturing rack of culturing room；
F, management：Temperature control, after planting to the stage of emerging, it is 13~18 DEG C to keep culture indoor temperature, and seedling stage keeps
Temperature is 17~22 DEG C, and knot potato phase keeping temperature is 13~19 DEG C；Moisture is controlled, and is after planting poured once daily to the stage of emerging
Water, keeps matrix moistening, and plant strain growth pours a water every other day to envelope woods from after emerging, and when blade starts to turn yellow, stops pouring
Water；
G, results：After matrix is dry, plant is lifted, harvest potato seed, the potato seed of same combination is mounted in same sand net bag
It is interior, hang up properly label preservation.
The indoor culture method of foregoing white potato seedling potato seed, the seedlings nursing plate selects more than 70mm high, cave when selecting
Bore 65mm on hole, the hole tray of cavities depth 70mm loads to the 65mm positions of seedlings nursing plate hole bottom matrix before sowing.
Beneficial effects of the present invention：Compared with prior art, it is of the invention to this have the advantage that：
(1) current year's production goes out the potato that grows directly from seeds after harvesting true seed then, and more than 1 year is shortened than existing breeding technique, improves
Breeding efficiency；
(2) the small potato that grows directly from seeds is directly produced in seedlings nursing plate, is reduced and is transplanted recruitment, save production of seed stock land used；
(3) directly cultivated in seedlings nursing plate and grown directly from seeds potato, it is to avoid be dead during small transplantation of seedlings and cause breeding gene type to damage
Lose and influence breeding results；
(4) set up individual plant mirror using the potato that grows directly from seeds and select garden, each individual plant can be fully showed the good and bad proterties of itself, it is to avoid
Individual plant mirror is directly set up using seedling to occur when selecting garden because that culturing and transplanting seedlings process seedling sustains damage is plain caused by depauperation
Choosing and wrong choosing, are selected in the degree of accuracy high；
(5) the soil pest and disease damage problem that conventional cultivation mode causes is avoided；
(6) the local natural resources pine needle detritus soil of matrix utilization, the nutrition supply plant strain growth being rich in using detritus soil, no
Drawn materials easily with fertilising, and matrix, low cost.
My company is contrasted the breeding method with other breeding methods, and comparing result is as shown in table 1.
As shown in Table 1, the planting percent and survival rate of other method for culturing seedlings are respectively less than 90%, and breeding method of the invention
Planting percent and survival rate are more than 99%, are greatly enhanced Potato Breeding efficiency.
Specific embodiment
With reference to embodiment, the present invention is further illustrated, but is not intended as the foundation to present invention limitation.
The present invention is carried out in the Hengfeng Science and Technology Development Co., Ltd. tissue culture laboratory of Guizhou, but the content control environment invented
It is not limited solely to the laboratory.
Embodiments of the invention 1：A kind of indoor culture method of white potato seedling potato seed, includes following steps：
A, matrix prepare：Matrix is the pine needle detritus soil rich in abundant nutrition organic matter, with the sieve that mesh is 10~15mm
Son sieving, and disinfected；Carry out seedlings nursing plate preparation simultaneously：Seedlings nursing plate is hole tray, and specification can be according to specific for long and width
Situation is optional, more than 70mm high, bore 65mm in cavities, depth 70mm.
The acquisition of B, hybridization true seed：On March 5th, 2015 sows parent, and early June starts preparing hybrid combination, August
Fruit of growing directly from seeds for 2nd harvests hybridization when cyan is changed into golden yellow and grows directly from seeds really.
C, wash seeds, the hybridization of 2 combinations of August harvesting on the 2nd is grown directly from seeds really totally 50, and the fruit of growing directly from seeds of each combination is put
In a stainless steel disc, wet towel being covered respectively, being placed in tissue culture room, allow its abundant after-ripening, checked at any time, when growing directly from seeds, fruit becomes
It is soft, when gently extruding is i.e. broken, cleaned within 30th in August, obtain true seed.
D, abolish true seed rest period, September 1 day, two seed amounts of combination are checked respectively, combine 1 quantity 3856
Grain, combines 2 quantity 4055, and then seed is wrapped with gauze respectively, and the gibberellin of 1000ppm is put into during the September morning 9 on the 2nd
Soaked in liquid, picked up during September 3 days 3.
E, presprouting of seeds, September will be placed in 26 DEG C or so the interiors of temperature on 3rd and carries out vernalization by the seed of seed soaking,
Bag is once planted with 26 DEG C or so of warm water washing daily, and checks Seed sprouting situation, September seed on the 9th shows money or valuables one carries unintentionally.
Matrix is loaded F, sowing, September 10 days the position of 65mm in seedlings nursing plate hole, is then sowed at seed with tweezers
In hole tray, per bunch planting one seed of kind, matrix is after planting covered, matrix lid is neat with card, combination code name is sticked on disk and is broadcast
Date tag is planted, then pours permeable, be placed on culturing rack.
G, management：Temperature control, culturing room adjusts temperature using air-conditioning, after planting to the stage of emerging, keeping temperature 13~
18℃；17~22 DEG C of seedling stage keeping temperature；13~19 DEG C of knot potato phase keeping temperature.Moisture is controlled, and a water is poured daily, keeps base
Matter is moistened.Plant strain growth pours a water every other day to after sealing woods (can't see card) after emerging, when starting to turn yellow to blade, 2016
On January 10, in stops watering.
H, results：After matrix is dry, on January 25th, 2016, subassembly is harvested, and lifts plant, and every plant harvests one
The good potato seed of Comprehensive Traits, the potato seed of same combination hangs up properly label preservation in same sand net bag.
Embodiments of the invention 2：A kind of indoor culture method of white potato seedling potato seed, includes following steps：
A, matrix prepare：Matrix is the pine needle detritus soil rich in abundant nutrition organic matter, with the sieve that mesh is 10~15mm
Son sieving, and disinfected；Carry out seedlings nursing plate preparation simultaneously：Seedlings nursing plate is hole tray, and specification can be according to specific for long and width
Situation is optional, more than 70mm high, bore 65mm in cavities, depth 70mm.
The acquisition of B, hybridization true seed：On 2 28th, 2014 sowing parents, early June starts preparing hybrid combination, 7
Fruit of growing directly from seeds for 29th moon harvests hybridization when cyan is changed into golden yellow and grows directly from seeds really.
C, wash seeds, the hybridization of 2 combinations that July 29 was harvested are grown directly from seeds really totally 48, the fruit of growing directly from seeds of each combination
It is placed in a stainless steel disc, wet towel is covered respectively, be placed in tissue culture room, allow its abundant after-ripening, check at any time, when fruit of growing directly from seeds
Soften, when gently extruding is i.e. broken, cleaned within 27th in August, obtain true seed.
D, abolish true seed rest period, September 5 days, two seed amounts of combination are checked respectively, combine 1 quantity 3658
Grain, combines 2 quantity 4117, and then seed is wrapped with gauze respectively, and the gibberellin of 1100ppm is put into during the September morning 9 on the 7th
Soaked in liquid, picked up during September 8 days 3.
E, presprouting of seeds, September will be placed in 26 DEG C or so the interiors of temperature on 8th and carries out vernalization by the seed of seed soaking,
Bag is once planted with 26 DEG C or so of warm water washing daily, and checks Seed sprouting situation, September seed on the 15th shows money or valuables one carries unintentionally.
Matrix is loaded F, sowing, September 16 days the position of 65mm in seedlings nursing plate hole, is then sowed at seed with tweezers
In hole tray, per bunch planting one seed of kind, matrix is after planting covered, matrix lid is neat with card, combination code name is sticked on disk and is broadcast
Date tag is planted, then pours permeable, be placed on culturing rack.
G, management：Temperature control, culturing room adjusts temperature using air-conditioning, after planting to the stage of emerging, keeping temperature 13~
18℃；17~22 DEG C of seedling stage keeping temperature；13~19 DEG C of knot potato phase keeping temperature.Moisture is controlled, and a water is poured daily, keeps base
Matter is moistened.Plant strain growth after can't see card, pours a water every other day to envelope woods after emerging, and on January 15th, 2015 starts when blade
During flavescence, stop watering.
H, results：After matrix is dry, on January 29th, 2016, subassembly is harvested, and lifts plant, and every plant harvests one
The good potato seed of Comprehensive Traits, the potato seed of same combination hangs up properly label preservation in same sand net bag, harvests seedling seed tuber
1 quantity 3653 is combined, 2 quantity 4112 is combined.
Embodiments of the invention 3：A kind of indoor culture method of white potato seedling potato seed, includes following steps：
A, matrix prepare：Matrix is the pine needle detritus soil rich in abundant nutrition organic matter, with the sieve that mesh is 10~15mm
Son sieving, and disinfected；Carry out seedlings nursing plate preparation simultaneously：Seedlings nursing plate is hole tray, and specification can be according to specific for long and width
Situation is optional, more than 70mm high, bore 65mm in cavities, depth 70mm.
The acquisition of B, hybridization true seed：On March 5th, 2015 sows parent, and early June starts preparing hybrid combination, August
Fruit of growing directly from seeds for 2nd harvests hybridization when cyan is changed into golden yellow and grows directly from seeds really.
C, wash seeds, the hybridization of 2 combinations of August harvesting on the 2nd is grown directly from seeds really totally 50, and the fruit of growing directly from seeds of each combination is put
In a stainless steel disc, wet towel being covered respectively, being placed in tissue culture room, allow its abundant after-ripening, checked at any time, when growing directly from seeds, fruit becomes
It is soft, when gently extruding is i.e. broken, cleaned within 30th in August, obtain true seed.
D, abolish true seed rest period, September 1 day, two seed amounts of combination are checked respectively, combine 1 quantity 3856
Grain, combines 2 quantity 4055, and then seed is wrapped with gauze respectively, and the gibberellin of 1200ppm is put into during the September morning 9 on the 2nd
Soaked in liquid, picked up during September 3 days 3.
E, presprouting of seeds, September will be placed in 26 DEG C or so the interiors of temperature on 3rd and carries out vernalization by the seed of seed soaking,
Bag is once planted with 26 DEG C or so of warm water washing daily, and checks Seed sprouting situation, September seed on the 9th shows money or valuables one carries unintentionally.
Matrix is loaded F, sowing, September 10 days the position of 65mm in seedlings nursing plate hole, is then sowed at seed with tweezers
In hole tray, per bunch planting one seed of kind, matrix is after planting covered, matrix lid is neat with card, combination code name is sticked on disk and is broadcast
Date tag is planted, then pours permeable, be placed on culturing rack.
G, management：Temperature control, culturing room adjusts temperature using air-conditioning, after planting to the stage of emerging, keeping temperature 13~
18℃；17~22 DEG C of seedling stage keeping temperature；13~19 DEG C of knot potato phase keeping temperature.Moisture is controlled, and a water is poured daily, keeps base
Matter is moistened.Plant strain growth can't see card to envelope woods after emerging) after, a water is poured every other day, the blade of on January 10th, 2016 starts
During flavescence, stop watering.
H, results：After matrix is dry, on January 25th, 2016, subassembly is harvested, and lifts plant, and every plant harvests one
The good potato seed of Comprehensive Traits, the potato seed of same combination hangs up properly label preservation in same sand net bag, harvests seedling seed tuber
Amount combination 1 quantity 3854, combines 2 quantity 4053.
Claims (3)
1. a kind of indoor culture method of white potato seedling potato seed, it is characterised in that：The method includes following steps：
A, matrix prepare：Pine needle detritus soil mesh is the sieve of 10~15mm, and as base after being disinfected
Matter；
B, abolish true seed rest period：Harvested after fruit maturation of growing directly from seeds, be subsequently placed in hole plate seedling growth disk, cover wet towel, put
Allow its after-ripening at room temperature, and check at any time, fruit softens when growing directly from seeds, cleaned when gently extruding is i.e. broken, after obtaining true seed, use
Gauze wraps seed, is put into the gibberellin liquid of 1000-1200ppm and soaks 28-32 hours, breaking dormancy；
D, presprouting of seeds：Vernalization is carried out by the 23-28 DEG C of interior of temperature is placed in by the seed of seed soaking, 23-28 is used daily
DEG C warm water washing once plant bag, and check Seed sprouting situation, after seed shows money or valuables one carries unintentionally by sow；
E, sowing：Matrix is loaded in seedlings nursing plate hole before sowing, then seed is sowed in hole tray, after planting cover matrix,
Pour permeable again, be placed on the culturing rack of culturing room；
F, management：Temperature control, after planting to the stage of emerging, it is 13~18 DEG C, seedling stage keeping temperature to keep culture indoor temperature
It it is 17~22 DEG C, knot potato phase keeping temperature is 13~19 DEG C；Moisture is controlled, and after planting pours a water daily to the stage of emerging, and is protected
Matrix moistening is held, plant strain growth pours a water every other day to envelope woods from after emerging, when blade starts to turn yellow, stop watering；
G, results：After matrix is dry, plant is lifted, harvest potato seed, the potato seed of same combination is hung in same sand net bag
Good label is preserved.
2. the indoor culture method of white potato seedling potato seed according to claim 1, it is characterised in that：The method is included
Following steps：
A, matrix prepare：Pine needle detritus soil mesh is the sieve of 10~15mm, and as base after being disinfected
Matter；
B, abolish true seed rest period：Harvested after fruit maturation of growing directly from seeds, be subsequently placed in hole plate seedling growth disk, cover wet towel, put
Allow its after-ripening at room temperature, and check at any time, fruit softens when growing directly from seeds, cleaned when gently extruding is i.e. broken, after obtaining true seed, use
Gauze wraps seed, is put into the gibberellin liquid of 1100ppm and soaks 30 hours, breaking dormancy；
D, presprouting of seeds：Vernalization is carried out by 26 DEG C of interiors of temperature are placed in by the seed of seed soaking, daily with 26 DEG C of temperature
Water is rinsed once plants bag, and checks Seed sprouting situation, can sowing after seed shows money or valuables one carries unintentionally；
E, sowing：Matrix is loaded in seedlings nursing plate hole before sowing, then seed is sowed in hole tray, per bunch planting one kind of kind
Son, after planting covers matrix, then pours permeable, and matrix lid is neat with card, is placed on the culturing rack of culturing room；
F, management：Temperature control, after planting to the stage of emerging, it is 13~18 DEG C, seedling stage keeping temperature to keep culture indoor temperature
It it is 17~22 DEG C, knot potato phase keeping temperature is 13~19 DEG C；Moisture is controlled, and after planting pours a water daily to the stage of emerging, and is protected
Matrix moistening is held, plant strain growth pours a water every other day to envelope woods from after emerging, when blade starts to turn yellow, stop watering；
G, results：After matrix is dry, plant is lifted, harvests potato seed, the potato seed of same combination is mounted in same sand net bag,
Hang up properly label preservation.
3. the indoor culture method of white potato seedling potato seed according to claim 1 and 2, it is characterised in that：The nursery
Disk selects more than 70mm high when selecting, bore 65mm in cavities, the hole tray of cavities depth 70mm, loads to educating matrix before sowing
At the 65mm positions of seed plate hole bottom.
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| CN107926207A (en) * | 2018-01-10 | 2018-04-20 | 和县绿源蔬菜种植有限责任公司 | Germination accelerating method in a kind of seed potato room |
| CN107950399A (en) * | 2017-12-28 | 2018-04-24 | 绵阳市农业科学研究院 | A kind of method for improving Some New True Potato Seed utilization and screening efficiency |
| CN108260487A (en) * | 2018-01-25 | 2018-07-10 | 李亚杰 | A kind of interior white potato seedling seeding cultivating method |
| CN108935043A (en) * | 2018-07-19 | 2018-12-07 | 河北科技师范学院 | A method of promote prematurity Some New True Potato Seed to sprout and tie micro potato |
| CN110604050A (en) * | 2019-10-31 | 2019-12-24 | 贵州恒丰科技开发有限公司 | Potato hybrid seedling seed bean seed coating direct seeding method |
| CN111656903A (en) * | 2020-06-12 | 2020-09-15 | 内蒙古大学 | Rapid and effective germination accelerating method for potato seedling seeds |
| CN116406597A (en) * | 2023-05-08 | 2023-07-11 | 龙岩市农业科学研究所 | Early-generation screening method for potato seedlings |
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| CN108260487A (en) * | 2018-01-25 | 2018-07-10 | 李亚杰 | A kind of interior white potato seedling seeding cultivating method |
| CN108935043A (en) * | 2018-07-19 | 2018-12-07 | 河北科技师范学院 | A method of promote prematurity Some New True Potato Seed to sprout and tie micro potato |
| CN110604050A (en) * | 2019-10-31 | 2019-12-24 | 贵州恒丰科技开发有限公司 | Potato hybrid seedling seed bean seed coating direct seeding method |
| CN111656903A (en) * | 2020-06-12 | 2020-09-15 | 内蒙古大学 | Rapid and effective germination accelerating method for potato seedling seeds |
| CN116406597A (en) * | 2023-05-08 | 2023-07-11 | 龙岩市农业科学研究所 | Early-generation screening method for potato seedlings |
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Application publication date: 20170613 |