CN106818120A - A kind of method for transplanting of bitter water rose tissue-cultured seedling - Google Patents
A kind of method for transplanting of bitter water rose tissue-cultured seedling Download PDFInfo
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- CN106818120A CN106818120A CN201611210174.6A CN201611210174A CN106818120A CN 106818120 A CN106818120 A CN 106818120A CN 201611210174 A CN201611210174 A CN 201611210174A CN 106818120 A CN106818120 A CN 106818120A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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Abstract
The invention provides a kind of method for transplanting of bitter water rose tissue-cultured seedling, belong to plant tissue and breed field.The method for transplanting is comprised the step of：(1) nutrient solution is prepared；(2) transplanting breeding；(3) protection is cultivated.Compared with prior art, the present invention turns out the seedlings root prosperity for coming, and stalk is sturdy, and cauline leaf is in great numbers, adaptable after transplanting, is that the field planting of next step bitter water rose is laid a good foundation；Simple to operate, efficiency high, the seedlings nursing plate for being used can be repeatedly used, and greatly reduce cost, can efficiently control the propagation of bacterial disease, and the growing environment for tissue-cultured seedling also can accurately be regulated and controled.
Description
Technical field
Field is bred the invention belongs to plant tissue, and in particular to a kind of method for transplanting of bitter water rose tissue-cultured seedling.
Background technology
Bitter water rose, be it is a kind of abound with the rose in Yongdeng County, Gansu Province county misery town, after the cultivation of more than 200 years, form
Well-known " bitter water rose " brand, is characterized in spending that numerous juice is more, delicate fragrance is pure, and " Chinese rose is also enjoyed in misery town therefore
The good reputation in the first township ".With the deep level development continued to develop with bitter water rose of economic society, the economic worth of bitter water rose
Increasingly highlighted with social value, added value of product more and more higher, purposes is more and more extensive, viewed and admired as collection, oil use, food
With, medicinal, wine brewing and the multiple use specialty economies kind such as spices.
The propagation method of bitter water rose is mainly cutting propagation, and survival rate is low, and rootage duration is long, and under offspring's quality
Drop.Also have what is bred using method for plant tissue culture at present, but tissue-cultured seedling transplanting survival rate is low, strongly limit hardship
The factorial praluction of water rose.
The content of the invention
The technical problems to be solved by the invention are directed to the deficiency or defect of prior art presence, there is provided a kind of misery rose
The method for transplanting of rare tissue-cultured seedling, to improve the survival rate of bitter water rose.
A kind of method for transplanting of bitter water rose tissue-cultured seedling, comprises the following steps：
(1) nutrient solution is prepared
MS fluid nutrient mediums and DKW fluid nutrient mediums are pressed 1:1~2 volume ratio is well mixed, and obtains mixed liquor A, will
Biogas slurry and sterilized water press 1:2~4 volume ratio is well mixed, and obtains mixed liquid B, and mixed liquor A and B are pressed into 1:2~3 volume ratio
It is well mixed to be made nutrient solution；
The biogas slurry is the refined filtration biogas slurry of sterilization treatment again after biogas slurry stoste is filtered through 100~150 mesh；
(2) transplanting breeding
Bitter water rose tissue-cultured seedling takes out tissue-cultured seedling in greenhouse hardening 10 days from blake bottle, with clear water by root culture medium
Wash clean, directly transplants in diaphragm seal in the hole tray equipped with transplanting medium, is added a cover, and pricks hole ventilation after 2 days on film, each
1~2 aperture is pricked around seedling at 2~5cm, one time of nutrition liquid is poured after transplanting, poured once at interval of 3~5 days thereafter, cultivation temperature
20~24 DEG C of degree, humidity 90%~95%, illumination 3000~5000lx, daily light application time 12h, after young leaves is extracted out, humidity drop
Low to 70~80%, illumination is enhanced to 6000~8000Lx, and cultivation temperature is 25~27 DEG C, daily light application time 8h；
Described transplanting medium is loessal soil, sierozem, vermiculite, bentonite, the mixed-matrix of river sand, and its volume ratio is 4
~5: 2~3: 1:3~4；
(3) protection is cultivated
Cultivate 15~20 days, remove film, sprayed once with 50% carbendazol wettable powder, 500~1000 times of liquid, its
Sprayed once with 50% carbendazol wettable powder, 500~1000 times of liquid every 5 days afterwards.
Advantageous Effects of the invention：Compared with prior art, the present invention turns out the seedlings root prosperity for coming, stalk
Sturdy, cauline leaf is in great numbers, adaptable after transplanting, is that the field planting of next step bitter water rose is laid a good foundation；It is simple to operate,
Efficiency high, the seedlings nursing plate for being used can be repeatedly used, and greatly reduce cost, can efficiently control bacterial disease
Propagate, the growing environment for tissue-cultured seedling also can accurately be regulated and controled.
Specific embodiment
The present invention is described in further detail with embodiment below, scope is not constituted to technical scheme to limitation.
Embodiment 1
A kind of method for transplanting of bitter water rose tissue-cultured seedling, comprises the following steps：
(1) nutrient solution is prepared
MS fluid nutrient mediums and DKW fluid nutrient mediums are pressed 1:1 volume ratio is well mixed, and mixed liquor A is obtained, by biogas slurry
1 is pressed with sterilized water:2 volume ratio is well mixed, and obtains mixed liquid B, and mixed liquor A and B are pressed into 1:The well mixed system of 2 volume ratio
Into nutrient solution；
The biogas slurry is the refined filtration biogas slurry of sterilization treatment again after biogas slurry stoste is filtered through 100 mesh；
(2) transplanting breeding
Bitter water rose tissue-cultured seedling takes out tissue-cultured seedling in greenhouse hardening 10 days from blake bottle, with clear water by root culture medium
Wash clean, directly transplants in diaphragm seal in the hole tray equipped with transplanting medium, is added a cover, and pricks hole ventilation after 2 days on film, each
1 aperture is pricked around seedling at 2cm, one time of nutrition liquid is poured after transplanting, poured once at interval of 3 days thereafter, 22 DEG C of growing temperatures are wet
Degree 90%, illumination 3000lx, daily light application time 12h, after young leaves is extracted out, humidity is reduced to 70%, and illumination is enhanced to 6000Lx,
Cultivation temperature is 25 DEG C, daily light application time 8h；
Described transplanting medium is loessal soil, sierozem, vermiculite, bentonite, the mixed-matrix of river sand, and its volume ratio is 4:
2∶1:3；
(3) protection is cultivated
Cultivate 15 days, remove film, sprayed once with 50% carbendazol wettable powder, 500 times of liquid, thereafter every 5 days use
50% 500 times of carbendazol wettable powder liquid is sprayed once.
Embodiment 2
A kind of method for transplanting of bitter water rose tissue-cultured seedling, comprises the following steps：
(1) nutrient solution is prepared
MS fluid nutrient mediums and DKW fluid nutrient mediums are pressed 1:2 volume ratio is well mixed, and mixed liquor A is obtained, by biogas slurry
1 is pressed with sterilized water:4 volume ratio is well mixed, and obtains mixed liquid B, and mixed liquor A and B are pressed into 1:The well mixed system of 3 volume ratio
Into nutrient solution；
The biogas slurry is the refined filtration biogas slurry of sterilization treatment again after biogas slurry stoste is filtered through 150 mesh；
(2) transplanting breeding
Bitter water rose tissue-cultured seedling takes out tissue-cultured seedling in greenhouse hardening 10 days from blake bottle, with clear water by root culture medium
Wash clean, directly transplants in diaphragm seal in the hole tray equipped with transplanting medium, is added a cover, and pricks hole ventilation after 2 days on film, each
2 apertures are pricked around seedling at 5cm, one time of nutrition liquid is poured after transplanting, poured once at interval of 5 days thereafter, 24 DEG C of growing temperatures are wet
Degree 95%, illumination 5000lx, daily light application time 12h, after young leaves is extracted out, humidity is reduced to 80%, and illumination is enhanced to 8000Lx,
Cultivation temperature is 27 DEG C, daily light application time 8h；
Described transplanting medium is loessal soil, sierozem, vermiculite, bentonite, the mixed-matrix of river sand, and its volume ratio is 5:
3∶1:4；
(3) protection is cultivated
Cultivate 20 days, remove film, sprayed once with 50% carbendazol wettable powder, 1000 times of liquid, thereafter every 5 days
Sprayed once with 50% carbendazol wettable powder, 1000 times of liquid.
Embodiment 3
A kind of method for transplanting of bitter water rose tissue-cultured seedling, comprises the following steps：
(1) nutrient solution is prepared
MS fluid nutrient mediums and DKW fluid nutrient mediums are pressed 2:3 volume ratio is well mixed, and mixed liquor A is obtained, by biogas slurry
1 is pressed with sterilized water:3 volume ratio is well mixed, and obtains mixed liquid B, and mixed liquor A and B are pressed into 2:The well mixed system of 5 volume ratio
Into nutrient solution；
The biogas slurry is the refined filtration biogas slurry of sterilization treatment again after biogas slurry stoste is filtered through 120 mesh；
(2) transplanting breeding
Bitter water rose tissue-cultured seedling takes out tissue-cultured seedling in greenhouse hardening 10 days from blake bottle, with clear water by root culture medium
Wash clean, directly transplants in diaphragm seal in the hole tray equipped with transplanting medium, is added a cover, and pricks hole ventilation after 2 days on film, each
2 apertures are pricked around seedling at 4cm, one time of nutrition liquid is poured after transplanting, poured once at interval of 4 days thereafter, 22 DEG C of growing temperatures are wet
Degree 95%, illumination 4000lx, daily light application time 12h, after young leaves is extracted out, humidity is reduced to 75%, and illumination is enhanced to 7000Lx,
Cultivation temperature is 26 DEG C, daily light application time 8h；
Described transplanting medium is loessal soil, sierozem, vermiculite, bentonite, the mixed-matrix of river sand, and its volume ratio is
4.5∶2.5∶1:3.5；
(3) protection is cultivated
Cultivate 18 days, remove film, sprayed once with 50% carbendazol wettable powder, 800 times of liquid, thereafter every 5 days use
50% 800 times of carbendazol wettable powder liquid is sprayed once.
Claims (1)
1. a kind of method for transplanting of bitter water rose tissue-cultured seedling, it is characterised in that comprise the following steps：
(1) nutrient solution is prepared
MS fluid nutrient mediums and DKW fluid nutrient mediums are pressed 1:1~2 volume ratio is well mixed, and mixed liquor A is obtained, by biogas slurry
1 is pressed with sterilized water:2~4 volume ratio is well mixed, and obtains mixed liquid B, and mixed liquor A and B are pressed into 1:2~3 volume ratio mixing
Uniformly it is made nutrient solution；
The biogas slurry is the refined filtration biogas slurry of sterilization treatment again after biogas slurry stoste is filtered through 100~150 mesh；
(2) transplanting breeding
Bitter water rose tissue-cultured seedling takes out tissue-cultured seedling in greenhouse hardening 10 days from blake bottle, and root culture medium is washed into dry with clear water
Only, directly transplant in diaphragm seal in the hole tray equipped with transplanting medium, is added a cover, prick hole ventilation, each seedling after 2 days on film
1~2 aperture is pricked at 2~5cm of surrounding, one time of nutrition liquid is poured after transplanting, poured once at interval of 3~5 days thereafter, growing temperatures 20
~24 DEG C, humidity 90~95%, illumination 3000~5000lx, daily light application time 12h, after young leaves is extracted out, humidity is reduced to 70
~80%, illumination is enhanced to 6000~8000Lx, and cultivation temperature is 25~27 DEG C, daily light application time 8h；
Described transplanting medium is loessal soil, sierozem, vermiculite, bentonite, the mixed-matrix of river sand, and its volume ratio is 4~5: 2
~3: 1:3~4；
(3) protection is cultivated
Cultivate 15~20 days, remove film, sprayed once with 50% carbendazol wettable powder, 500~1000 times of liquid, thereafter often
Sprayed once with 50% carbendazol wettable powder, 500~1000 times of liquid every 5 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611210174.6A CN106818120A (en) | 2016-12-24 | 2016-12-24 | A kind of method for transplanting of bitter water rose tissue-cultured seedling |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611210174.6A CN106818120A (en) | 2016-12-24 | 2016-12-24 | A kind of method for transplanting of bitter water rose tissue-cultured seedling |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106818120A true CN106818120A (en) | 2017-06-13 |
Family
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Family Applications (1)
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| CN201611210174.6A Pending CN106818120A (en) | 2016-12-24 | 2016-12-24 | A kind of method for transplanting of bitter water rose tissue-cultured seedling |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102090329A (en) * | 2010-11-10 | 2011-06-15 | 天津滨海国际花卉科技园区股份有限公司 | Method for transplanting Damascus rose tissue culture seedling |
| CN102217538A (en) * | 2011-05-17 | 2011-10-19 | 山西省农业科学院果树研究所 | Method for treating walnut tissue culture stem segments inside test tubes and rooting outside test tubes |
| CN105284572A (en) * | 2015-11-26 | 2016-02-03 | 广东粤三胖农业科技有限责任公司 | Rose tissue culture seedling transplanting method and matrix thereof |
| CN105503359A (en) * | 2016-03-02 | 2016-04-20 | 甘肃新明生物科技有限公司 | Bone-powder fertilizer for Kushui rose and preparation method of bone-powder fertilizer |
| CN105815057A (en) * | 2015-01-05 | 2016-08-03 | 达金兰 | Ku-shui rose cultivation method |
-
2016
- 2016-12-24 CN CN201611210174.6A patent/CN106818120A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102090329A (en) * | 2010-11-10 | 2011-06-15 | 天津滨海国际花卉科技园区股份有限公司 | Method for transplanting Damascus rose tissue culture seedling |
| CN102217538A (en) * | 2011-05-17 | 2011-10-19 | 山西省农业科学院果树研究所 | Method for treating walnut tissue culture stem segments inside test tubes and rooting outside test tubes |
| CN105815057A (en) * | 2015-01-05 | 2016-08-03 | 达金兰 | Ku-shui rose cultivation method |
| CN105284572A (en) * | 2015-11-26 | 2016-02-03 | 广东粤三胖农业科技有限责任公司 | Rose tissue culture seedling transplanting method and matrix thereof |
| CN105503359A (en) * | 2016-03-02 | 2016-04-20 | 甘肃新明生物科技有限公司 | Bone-powder fertilizer for Kushui rose and preparation method of bone-powder fertilizer |
Non-Patent Citations (1)
| Title |
|---|
| 郭倩: "不同培养基对‘洛阳红’牡丹试管苗生长的影响", 《农业科技辑》 * |
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Application publication date: 20170613 |
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