CN105123512B - A kind of breeding method of roxburgh anoectochilus terminal bud - Google Patents
A kind of breeding method of roxburgh anoectochilus terminal bud Download PDFInfo
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- CN105123512B CN105123512B CN201510416260.1A CN201510416260A CN105123512B CN 105123512 B CN105123512 B CN 105123512B CN 201510416260 A CN201510416260 A CN 201510416260A CN 105123512 B CN105123512 B CN 105123512B
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Abstract
The invention discloses a kind of breeding method of roxburgh anoectochilus terminal bud, comprise the following steps：Step 1, the preparation of culture medium；Step 2, materials disinfection processing；Step 3, Fiber differentiation；Step 4, breed culture of rootage；Step 5, transplanting.The breeding method of the roxburgh anoectochilus terminal bud of the present invention, stem section can sprout the strong sprouting of key for 15 days by Fiber differentiation；Again by propagation culture of rootage 30 50d, obtain the plant that can be transplanted, rooting rate is up to 99%, this method culture generation time is short, expands that numerous coefficient height, one-step-seedling formation, seedling are healthy, roxburgh anoectochilus terminal bud stem plant is thick, well developed root system, survival rate is high, leaf color is deep obvious flannelette shape, vein line is apparent, with significant economic benefit, the excellent purifying tissue-cultured seedling of large area plantation can be provided, pass through the carry out Fiber differentiation of circulation, the speed cultivated can be accelerated, it is not necessary to reprocess material.
Description
Technical field
The present invention relates to the breeding method of plant, particularly relates to a kind of breeding method of roxburgh anoectochilus terminal bud.
Background technology
Roxburgh anoectochilus terminal bud seed is smaller, is made up of immature embryo and several layers of kind chrotoplast, its natural germination rate and low reproduction rate,
And then the destruction due to Natural Survival environment and immoderate harvesting are fewer and fewer for wild gold lotus, this largely hinders
Application of the roxburgh anoectochilus terminal bud on Chinese Medicine Industry.
Roxburgh anoectochilus terminal bud seed is small, is made up of immature embryo and several layers of kind chrotoplast, natural germination rate and low reproduction rate, raw
Seedling in production is all from tissue cultures.At present, the tissue culture of roxburgh anoectochilus terminal bud mainly by " induction of adventitious bud, bud propagation with
Strong seedling culture " three phases realize that incubation time is longer, cost is higher, cause roxburgh anoectochilus terminal bud production always cannot be faster
Development.Therefore, how to shorten incubation time, improve culture efficiency, reduce toxigenic capacity as promotion roxburgh anoectochilus terminal bud production development
The task of top priority.
The content of the invention
It is an object of the invention to propose a kind of breeding method of roxburgh anoectochilus terminal bud, solves roxburgh anoectochilus terminal bud of the prior art
Tissue culture cultivation period it is long the defects of.
The technical proposal of the invention is realized in this way：A kind of breeding method of roxburgh anoectochilus terminal bud, comprises the following steps：
Step 1, the preparation of culture medium：
Inducing culture：Modified MS medium+0.5mg/L 6-BA+0.3mg/L IBA+50g/L banana extracts；
Breed root media：Using improvement modified MS medium+0.6mg/L NAA+0.3mg/L IBA+50g/L bananas
Extract；
Step 2, materials disinfection processing:The roxburgh anoectochilus terminal bud of collection is rinsed well with running water, takes 2-3 centimetres of stem section,
Unnecessary blade is removed, puts in sterilized water and scrubs clean, after scrubbing clean, with 75% alcohol disinfecting 30s, it is molten to add mercury chloride
Liquid handles 10-12min；Rushed 4-6 times with sterilized water again；
Step 3, Fiber differentiation：Material after sterilization is inoculated in through in high temperature, autoclaved inducing culture；Training
The condition of supporting：It is 25 ± 5 DEG C, light application time 12h/d, intensity of illumination 500-1000lx to cultivate room temperature, bud inducement cultivation 15 days；
To Multiple Buds；
Step 4, breed culture of rootage：It will be inoculated with after the Multiple Buds obtained by step 3 Fiber differentiation are cut into 1-2 centimetres
In root media is bred；Stem section is lain on culture medium during inoculation, breeds culture of rootage by 30-50d, cultivates bar
Part：It is 25 ± 5 DEG C, light application time 12h/d, intensity of illumination 500-1000lx to cultivate room temperature；Plant after being taken root；
Step 5, transplanting：Plant after taking root is transferred in the matrix that peat soil and fertile soil are mixed into, cultivation temperature
25~30 DEG C, humidity is maintained between 60%-75%, daily illumination 9~13 hours, 1500~2000lx of intensity of illumination.
Preferably, the activated carbon for accounting for that the culture medium mass percent is 1%-5% is added in the inducing culture.
Preferably, it is 1%-5% to be added in the propagation culture of rootage culture medium and account for the culture medium mass percent
Activated carbon.
Preferably, plant step 4 obtained be transferred to repeat step three to step 4 in inducing culture obtain it is removable
The plant of cultivation.
Beneficial effects of the present invention are：The breeding method of the roxburgh anoectochilus terminal bud of the present invention, stem section can be sprouted for 15 days by Fiber differentiation
Send the strong sprouting of key；Again by propagation culture of rootage 30-50d, the plant that can be transplanted is obtained, rooting rate is up to 99%, the party
Method culture generation time is short, expands that numerous coefficient height, one-step-seedling formation, seedling are healthy, roxburgh anoectochilus terminal bud stem plant is thick, well developed root system, into
Motility rate is high, and leaf color is deep obvious flannelette shape, and vein line is apparent, has significant economic benefit, can provide big face
The excellent purifying tissue-cultured seedling of product plantation, by the carry out Fiber differentiation of circulation, the speed bred can be accelerated.
Embodiment
To more fully understand the present invention, make further specific elaboration, but not to the present invention below by following examples
Limitation of the invention is can be regarded as, some made for those skilled in the art according to foregoing invention content are nonessential
Improve and adjust, be also considered as being within the scope of the present invention.
A kind of breeding method of roxburgh anoectochilus terminal bud, comprises the following steps：
Step 1, the preparation of culture medium：
Inducing culture：Modified MS medium+0.5mg/L 6-BA+0.3mg/L IBA+50g/L banana extracts+1g/L
Activated carbon；
Breed root media：Using improvement modified MS medium+0.6mg/L NAA+0.3mg/L IBA+50g/L bananas
Extract+2g/L activated carbons；
Step 2, materials disinfection processing:The roxburgh anoectochilus terminal bud of collection is rinsed well with running water, 2 centimetres of stem section is taken, goes
Except unnecessary blade, put in sterilized water and scrub clean, after scrubbing clean, with 75% alcohol disinfecting 30s, add mercuric chloride solution
Handle 10min；Rushed 4 times with sterilized water again；
Step 3, Fiber differentiation：Material after sterilization is inoculated in through in high temperature, autoclaved inducing culture；Training
The condition of supporting：It is 25 ± 5 DEG C, light application time 12h/d, intensity of illumination 500-1000lx to cultivate room temperature, bud inducement cultivation 15 days；
To Multiple Buds；
Step 4, breed culture of rootage：It will be inoculated with after the Multiple Buds obtained by step 3 Fiber differentiation are cut into 1-2 centimetres
In root media is bred；Stem section is lain on culture medium during inoculation, breeds culture of rootage by 30-50d, cultivates bar
Part：It is 25 ± 5 DEG C, light application time 12h/d, intensity of illumination 500-1000lx to cultivate room temperature；Plant after being taken root；By one
Plant after part is taken root is inoculated into inducing culture as material；
Step 5, transplanting：Plant after taking root is transferred in the matrix that peat soil and fertile soil are mixed into, cultivation temperature
25~30 DEG C, humidity is maintained between 60%-75%, daily illumination 9~13 hours, 1500~2000lx of intensity of illumination.
Raw material of the plant as Fiber differentiation of culture of rootage acquisition will be bred, accelerate the speed of culture, propagation life
The step of root culture one is completed, and shortens cycle of breeding, the addition of the preparation, particularly activated carbon of each composition in culture medium so that
The plant of cultivation is more sturdy, well developed root system, and survival rate is high, has significant economic benefit.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
God any modification, equivalent substitution and improvements made etc., should be included in the scope of the protection with principle.
Claims (4)
1. a kind of breeding method of roxburgh anoectochilus terminal bud, it is characterised in that comprise the following steps：
Step 1, the preparation of culture medium：
Inducing culture：Modified MS medium+0.5mg/L 6-BA+0.3mg/L IBA+50g/L banana extracts；
Breed root media：Using modified MS medium+0.6mg/L NAA+0.3mg/L IBA+50g/L banana extracts；
Step 2, materials disinfection processing:The roxburgh anoectochilus terminal bud of collection is rinsed well with running water, takes 2-3 centimetres of stem section, is removed
Unnecessary blade, puts in sterilized water and scrubs clean, and after scrubbing clean, with 75% alcohol disinfecting 30s, adds at mercuric chloride solution
Manage 10-12min；Rushed 4-6 times with sterilized water again；
Step 3, Fiber differentiation：Material after sterilization is inoculated in through in high temperature, autoclaved inducing culture；Cultivate bar
Part：It is 25 ± 5 DEG C, light application time 12h/d, intensity of illumination 500-1000lx to cultivate room temperature, bud inducement cultivation 15 days；Obtain clump
Sprout；
Step 4, breed culture of rootage：Increasing will be seeded in after the Multiple Buds obtained by step 3 Fiber differentiation are cut into 1-2 centimetres
Grow in root media；Stem section is lain on culture medium during inoculation, breeds culture of rootage, condition of culture by 30-50d：Training
It is 25 ± 5 DEG C, light application time 12h/d, intensity of illumination 500-1000lx to support room temperature；Plant after being taken root；
Step 5, transplanting：Plant after taking root is transferred in the matrix that peat soil and fertile soil are mixed into, cultivation temperature 25~
30 DEG C, humidity is maintained between 60%-75%, daily illumination 9~13 hours, 1500~2000lx of intensity of illumination.
2. the breeding method of roxburgh anoectochilus terminal bud as claimed in claim 1, it is characterised in that：Added in the inducing culture described in accounting for
Culture medium mass percent is 1%-5% activated carbon.
3. the breeding method of roxburgh anoectochilus terminal bud as claimed in claim 1, it is characterised in that：Add in the propagation culture of rootage culture medium
The activated carbon that the culture medium mass percent is 1%-5% is entered to account for.
4. the breeding method of roxburgh anoectochilus terminal bud as claimed in claim 1, it is characterised in that：The plant that step 4 is obtained is as former material
Material is inoculated into repeat step three to step 4 in inducing culture and obtains the plant that can be transplanted.
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| CN201510416260.1A CN105123512B (en) | 2015-07-15 | 2015-07-15 | A kind of breeding method of roxburgh anoectochilus terminal bud |
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| CN105706936B (en) * | 2016-03-15 | 2017-11-24 | 福建农林大学 | A kind of method of organic curing matrix tissue culture roxburgh anoectochilus terminal bud |
| CN109042328A (en) * | 2018-09-07 | 2018-12-21 | 天津大学 | A kind of method of roxburgh anoectochilus terminal bud tissue-culturing quick-propagation |
| CN110915563B (en) * | 2019-11-07 | 2022-03-22 | 广东省农业科学院作物研究所 | Application of cytokinin 6-BA in prevention and treatment of high-temperature heat damage of anoectochilus formosanus |
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| CN101773069B (en) * | 2009-12-29 | 2012-12-26 | 华南农业大学 | Tissue culture rapid propagation method of Guangdong anoectochilus roxburghii |
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