CN110476809A - A kind of transportation resources of sugarcane tissue-culture rooted seedling - Google Patents
A kind of transportation resources of sugarcane tissue-culture rooted seedling Download PDFInfo
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- CN110476809A CN110476809A CN201910757981.7A CN201910757981A CN110476809A CN 110476809 A CN110476809 A CN 110476809A CN 201910757981 A CN201910757981 A CN 201910757981A CN 110476809 A CN110476809 A CN 110476809A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/55—Sugar cane
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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Abstract
The invention discloses a kind of transportation resources of sugarcane tissue-culture rooted seedling, comprising the following steps: acquisition, the preceding pretreatment of rooted seedling transport, outdoor temporary planting of sugarcane tissue-culture rooted seedling；When transport; using the acrylamide and acrylic acid salt cross-linked copolymer handled through sodium benzoate as protection matrix; sugarcane tissue-culture rooted seedling transportation cost can be greatly reduced using the transportation resources of the sugarcane tissue-culture rooted seedling and improve temporary planting survival rate; it is used cooperatively by the sodium benzoate of acrylamide and acrylic acid salt cross-linked copolymer and suitable concentration; good protective effect can be provided to sugar-cane tissue culture seedlings, the temporary planting survival rate after substantially increasing sugar-cane tissue culture seedlings long-distance transport.
Description
Technical field
The invention belongs to field of plant tissue culture technique, more particularly, to a kind of transport of sugarcane tissue-culture rooted seedling
Method.
Background technique
Sugarcane tissue culture is a kind of efficient reproduction technique, using sugarcane stem-apex Meristem culture is obtained without disease
The effective way of seedling.But the production base of sugarcane disease-free seedlings with need be planted and plant farm or sugarcane field often not same
As soon as area or city, this need disease-free sugarcane tissue-culture rooted seedling from production base transport to need be planted and plant farm or
Sugarcane field.Seedling is transported in culture bottle, although the damage to seedling is small but considerably increases the bottle seedling for needing to transport
Volume and weight, increase transportation cost.If removal culture bottle only transport tissue culture rooted seedling, although can reduce transport at
This, but seedling may survive during transport and storage because of water shortage, anoxic or mechanically damaged and dead to reduce temporary planting
Rate.So there is an urgent need to a kind of and the transportation cost of sugarcane tissue-culture rooted seedling can be reduced, but can effective protection seedling do not transporting
It comes to harm on the way, extends the rooted seedling resting period, the method for improving the temporary planting survival rate of rooted seedling after transporting.
Summary of the invention
The technical problem to be solved in the present invention is that being directed to the transportation resources of existing sugarcane tissue-culture rooted seedling in transportational process
Middle cost is excessively high and is planted the not high technological deficiency of survival rate, provides a kind of reduction sugarcane tissue-culture rooted seedling transportation cost and raising
It is planted the sugarcane tissue-culture rooted seedling transportation resources of survival rate.
The purpose of the present invention is achieved by the following technical programs:
There is provided a kind of transportation resources of sugarcane tissue-culture rooted seedling, comprising the following steps: acquisition, the life of sugarcane tissue-culture rooted seedling
Pretreatment, transport, outdoor temporary planting before offspring transport；Wherein, pretreatment is handled to use through antimicrobial fluid before the rooted seedling transport
Protection matrix of the acrylamide and acrylic acid salt cross-linked copolymer as sugarcane tissue-culture rooted seedling, to sugarcane tissue-culture rooted seedling into
Row protection.
Further, pretreated concrete operations before the rooted seedling is transported are as follows: the sugarcane tissue-culture through taming hardening is raw
Offspring takes out out of culture bottle, cleans culture medium on seedling with clear water, cuts off the leaf of half, downward by one end of long root, inclination
It is placed in the acrylamide and acrylic acid salt cross-linked copolymer plastic box that one layer is handled through antimicrobial fluid that tiled, with acrylamide-
Acrylates cross-linked copolymer package sugarcane takes root bottle seedling base portion, one layer of the covering plastics for having an air hole above the plastic box
Film.Since sugar-cane tissue culture seedlings are tufted seedlings, growing point is lower, and almost all concentrates on base portion, and all tillers are all
It grows from base portion.
Preferably, the partial size of the acrylamide and acrylic acid salt cross-linked copolymer is 1.5~2.0mm.Due to sugarcane tissue-culture
The false stem diameter of seedling is generally in 1~2mm or so, and in 4~8cm or so, plant is more very thin to be easily snapped off plant height, and is being transported
Be also required to during defeated so that sugar-cane tissue culture seedlings will not anoxic, which be the partial size of most suitable sugar-cane tissue culture seedlings.
Preferably, the antimicrobial fluid is PhCOONa solution.
Further, using the concrete operations of the acrylamide and acrylic acid salt cross-linked copolymer handled through antimicrobial fluid are as follows: In
The sodium benzoate that 0.5~3g/L is added in water is adjusted to pH 3.0~4.0, in mass ratio 1:50~100 until completely dissolved
Ratio acrylamide and acrylic acid salt cross-linked copolymer is mixed with PhCOONa solution, impregnate 60min.
Preferably, the number of plies that the sugarcane tissue-culture rooted seedling is placed is 2~5 layers.
It is highly preferred that the number of plies that the sugarcane tissue-culture rooted seedling is placed is 2~3 layers.
Further, the concrete operations of the acquisition of the sugarcane tissue-culture rooted seedling are as follows: cut sugarcane 1~2mm of sugarcane bud growth
The disease-free sugarcane that point is cultivated and obtained breeds bottle seedling, accesses in promoting root growth culture medium and is cultivated, promoting root growth culture to sugarcane group
Training seedling grows the root system of white health.
Preferably, the condition of culture are as follows: 2000~2500Lux of intensity of illumination, daily light application time 10~16 hours, temperature
25~30 DEG C of degree is cultivated 10~30 days；The formula of the promoting root growth culture medium are as follows: 50000mg/L sugarcane is added in 1/2MS culture medium
Sugar, 1.0~4.0mg/L indolebutyric acid, 0.5~2mg/L methyl α-naphthyl acetate, 1~3mg/L paclobutrazol.
Further, the concrete operations of the outdoor temporary planting are as follows: be crosslinked sugarcane rooted seedling from acrylamide and acrylic acid salt
It takes out, is divided into containing 1~4 plant of Cong Miao with root seedling in copolymer, impregnated 5 with carbendazim solution~carried out vacation after ten minutes
It plants, every 10 days or so the thin fertilizer of Shi Yici after 20~25 days seedlings start to turn green, and leaf-cutting promotees to strengthen.
Preferably, 50% carbendazol wettable powder of carbendazim solution, 800 to 1000 times of solution.
The beneficial effects of the present invention are:
1. providing a kind of method for reducing sugarcane tissue-culture rooted seedling transportation cost and improving temporary planting survival rate, it is good to obtain growth
After good, health of root prosperity health cane tissue-cultured seedling, transport from production base to the farm or sugarcane for needing temporary planting and plantation
During field, using the biocidal property of antistaling agent, the good moisture retention of acrylamide and acrylic acid salt cross-linked copolymer, acryloyl
Resiliency and acrylamide and acrylic acid salt cross-linked copolymer of the amine-acrylates crosslinked copolymer particles to mechanical pressure
The gas permeability in gap between grain makes sugarcane tissue-culture rooted seedling in transit not because of water shortage, anoxic, bacterium infection and by machinery
Damage by and it is dead, reduce rotten the case where, extend the resting period of rooted seedling, improve transport after seedling temporary planting survival rate it is same
When, and the transportation volume and weight of rooted seedling are effectively reduced, it is the popularization of sugarcane tissue-culture disease-free seedlings to reduce transportation cost
Improve sound assurance.The present invention is promoted the use of suitable for sugarcane R&D institution and sugarcane health seedling tissue culture factory etc..
2. sugar-cane tissue culture seedlings are tufted seedlings, growing point is lower, and almost all concentrates on base portion.So if base portion is long-distance
It is directly impregnated with water or fresh-keeping solution in transportational process, sugar-cane tissue culture seedlings base portion can be easily lead to because anoxic or mutual overstock are rubbed
Wiping leads to mechanical damage to substantially reduce temporary planting survival rate.If sugarcane takes root, tissue-cultured seedling band culture bottle is transported for long-distance,
Although playing a very good protection to seedling, the volume and weight of transport can but be greatly increased, to affect transport
Convenience and improve the cost of transport.
Detailed description of the invention
The case where Fig. 1 worker is divided into seedlings after concentrating at the scene to the sugarcane promoting root growth tissue-cultured seedling in transport case.
The upgrowth situation of Fig. 2 sugar-cane tissue culture seedlings.
The sugarcane tissue culture seedling provisonal planting of pretreated group survived situation after 20 days in Fig. 3 embodiment 1.
The sugarcane tissue culture seedling provisonal planting of control group one survived situation after 20 days in Fig. 4 embodiment 1.
The sugarcane tissue culture seedling provisonal planting of processing group one survived situation after 20 days in Fig. 5 embodiment 2.
The sugarcane tissue culture seedling provisonal planting of processing group two survived situation after 20 days in Fig. 6 embodiment 2.
Situation is survived after Guangdong No. 60 tissue-cultured seedling of sugar are planted 20 days in Fig. 7 embodiment 3.
Specific embodiment
Below in conjunction with specific embodiment, technical scheme is described further, but the present invention is not limited to this
A little specific embodiments.Material used in embodiment, reagent etc., are commercially available unless otherwise specified.
Sugar-cane tissue culture seedlings are tufted seedlings, and growing point is lower, and almost all concentrates on base portion, and all tillers be all from
What base portion grew.The false stem diameter of sugar-cane tissue culture seedlings generally in 1~2mm or so, plant height in 4~8cm or so, plant compared with
It is easily snapped off to be very thin, as shown in Fig. 2.
Embodiment 1
Breed out the tissue culture rooted seedling of 45000 plants of casting skin fruit sugarcane health seedlings from Guangzhou Inst of Cane Sugar, 2015 3
The moon transports seedling and is planted to Shaoguan Wengyuan sugarcane district, carries out as follows:
1) acquisition of sugarcane tissue-culture rooted seedling: will by hot water treatment and 40 DEG C of high temperature vernalization, cut sugarcane sugarcane bud 1~
The disease-free casting skin fruit sugarcane that 2mm growing point is cultivated and obtained breeds bottle seedling, accesses promoting root growth culture medium: 1/2MS+ sucrose
50000mg/L+IBA 2.0mg/L+NAA 1.0mg/L+ paclobutrazol 1.0mg/L, intensity of illumination 2000Lux, daily light application time
16 hours, 27 DEG C of temperature.Visible sugar-cane tissue culture seedlings grow the root system that color is whiter, healthy after promoting root growth culture 25 days.
2) rooted seedling transport before pretreatment: by through tame hardening the high about 3~5cm casting skin fruit sugarcane of false stem take root bottle seedling from
It is taken out in culture bottle, the culture medium on seedling is cleaned up with clear water, cuts off the leaf of half.Test group processing: long root
One end downward, is placed in the plastic box for the one layer of pretreated acrylamide and acrylic acid salt cross-linked copolymer that tiled.Pre- place
The acrylamide and acrylic acid salt cross-linked copolymer of reason is: the sodium benzoate of 1.5g/L, after being completely dissolved, PH being added in clear water
Value is adjusted to 3.5, and the ratio of 1:80 in mass ratio is the acrylamide and acrylic acid salt copolymerzation with cross-linking of 1.5~2.0mm particle diameter
Object is mixed with PhCOONa solution, impregnates 120min.One layer of the very thin lid in top after seedling is piled one layer in seedling is pretreated
Acrylamide and acrylic acid salt cross-linked copolymer finally covers one layer of plastic film for having air hole in the top of plastic casing again.
Originally the plastic crate of 60cm wide 42cm high 18cm one long can only load 96 bottles of promoting root growth bottle seedlings, and 45000 young plants are about 4500 bottles
Promoting root growth bottle seedling, this batch of seedling need to fill about 47 baskets, and the basket weight for each filling tissue-culture container seedling is about 25kg or so, and this batch Miao Lian bottles
The weight of transport is about 1175kg.One picking-up vehicle conveying carriage in non-overloading situation can load 12 baskets of seedlings, and 45000 plants
Seedling needs to transport in four times.Every time transport back and forth one time oily expense, travelling expenses, driver's labour cost adds up is approximately 1300 yuan, four times
It is 5200 yuan back and forth.And after processing, this batch of seedling only needs 12 baskets that can install, and volume is about reduced into original tape glass culture
25.5% when bottle, total weight only has about 300kg, substantially increases the convenience of transport.Picking-up vehicle transports one time can be
All seedlings are sent to sugarcane district, freight charges need to only be transported with bottle 1/4.Control group one: being directly added into the sodium benzoate of 1.5g/L with clear water,
After being completely dissolved, pH value is adjusted to 3.5.The root of fruit sugarcane rooted seedling is directly impregnated with PhCOONa solution.Control group two: without
Pretreatment, directly band culture bottle transport.
3) it outdoor temporary planting: is carried out since the pretreatment of casting skin fruit sugarcane rooted seedling is accomplished to and transports to Wengyuan sugarcane district formally false
It plants, seedling stored in plastic box or culture bottle more than 30 hours, and the seedling at this time with culture bottle transport still grows fine, seedling
Color is dark green, as shown in Fig. 1.The seedling being placed in acrylamide and acrylic acid salt cross-linked copolymer and the seedling transported with bottle are similar, face
Color is dark green, and there are also part new roots to have grown into crosslinking composition granule, and directly starts to send out with the seedling color that PhCOONa solution impregnates
Huang, it is longer without new root.It is unified to be divided into hand containing 1~4 plant of Cong Miao with root seedling after fruit sugarcane rooted seedling is taken out, it uses
50% 1000 times of carbendazol wettable powder liquid impregnates after ten minutes, and it is the base of 3:1 that seedling is planted in loam+filter mud by volume
In matter.Capping in 10 days hides film water conservation heat preservation after temporary planting, counts survival rate after 20 days seedlings start to turn green, and applied every 10 days
Primary thin fertilizer, leaf-cutting promote strong.Two survival rate of control group is 96.2%, and sugarcane seedling grows fine.Pretreated group sugarcane seedling growing way with compare
Group is two similar, and survival rate is up to 95.6%.One seedling growing way of control group is weaker, and survival rate only has 38.2%.Illustrate that acrylamide-is added
Acrylates cross-linked copolymer has extraordinary protective effect to sugarcane seedling, can greatly improve sugar-cane tissue culture seedlings after transporting for long-distance
It is planted survival rate.Situation is survived as shown in attached drawing 3,4 after the temporary planting of sugarcane seedling 20 days.
Embodiment 2
Breed out the tissue culture rooted seedling of 38000 plants of clausenae Lansium sugarcane health seedlings from Guangzhou Inst of Cane Sugar, 2016 3
The moon is transported to Shaoguan Wengyuan sugarcane district and is planted, and carries out as follows:
1) acquisition of sugarcane tissue-culture rooted seedling: will by hot water treatment and 40 DEG C of high temperature vernalization, cut sugarcane sugarcane bud 1~
The disease-free clausenae Lansium sugarcane that 2mm growing point is cultivated and obtained breeds bottle seedling, accesses promoting root growth culture medium: 1/2MS+ sucrose
50000mg/L+IBA 2.5mg/L+NAA 1.5mg/L+ paclobutrazol 1.2mg/L, intensity of illumination 2000Lux, daily light application time
16 hours, 27 DEG C of temperature.Visible sugar-cane tissue culture seedlings grow the root system that color is whiter, healthy after promoting root growth culture 20 days.
2) rooted seedling transport before pretreatment: by through tame hardening the high about 3~5cm clausenae Lansium sugarcane of false stem take root bottle seedling from
It is taken out in culture bottle, the culture medium on seedling is cleaned up with clear water, cuts off the leaf of half.Downward one end of long root, it puts
It is placed in the plastic box for the one layer of pretreated acrylamide and acrylic acid salt cross-linked copolymer that tiled.Pretreated acryloyl
Amine-acrylates cross-linked copolymer is: a certain amount of sodium benzoate is added in clear water, after being completely dissolved, pH value is adjusted to 3.5,
The ratio of 1:80 is the acrylamide and acrylic acid salt cross-linked copolymer and benzoic acid of 1.5~2.0mm particle diameter in mass ratio
Sodium solution mixing, impregnates 120min.Top one layer of pretreated acrylamide-the third of very thin lid after seedling is piled one layer in seedling
Olefin(e) acid salt cross-linked copolymer finally covers one layer of plastic film for having air hole in the top of plastic casing again.Test group processing:
Processing one is added the sodium benzoate of 1.5g/L in clear water, then mixes with acrylamide and acrylic acid salt cross-linked copolymer.Processing two,
The sodium benzoate of 5.0g/L is added in clear water, then is mixed with acrylamide and acrylic acid salt cross-linked copolymer.
3) it outdoor temporary planting: is carried out since the pretreatment of clausenae Lansium sugarcane rooted seedling is accomplished to and transports to Wengyuan sugarcane district formally false
It plants, seedling was stored in plastic box more than 24 hours.It is unified to be divided into hand containing 1~4 plant of band after fruit sugarcane rooted seedling is taken out
The Cong Miao of root seedling is impregnated after ten minutes with 50% carbendazol wettable powder, 1000 times of liquid, seedling is planted and is pressed in loam+filter mud
Volume ratio is in the matrix of 3:1.Capping in 10 days hides film water conservation heat preservation after temporary planting, counts and survives after 20 days seedlings start to turn green
Rate, and every the 10 days thin fertilizer of Shi Yici, leaf-cutting promotees strong.One survival rate of processing group is up to 95.8%, and seedling growing way is vigorous；Processing group
Two survival rates only have 72.3%, and growing way is slightly weak, illustrate that sodium benzoate concentration is unsuitable excessively high.Situation is survived after the temporary planting of sugarcane seedling 20 days
As shown in attached drawing 5,6.
Embodiment 3
From Guangzhou, the sugared No. 60 tissue culture rooted seedlings in 40000 plants of sugarcane Guangdong are bred out by Guangzhou Inst of Cane Sugar, and 2017 5
Sugar refinery Mao Yuan that the moon is transported to Shaoguan Wengyuan is planted, and is carried out as follows:
1) acquisition of sugarcane tissue-culture rooted seedling: will by hot water treatment and 40 DEG C of high temperature vernalization, cut sugarcane sugarcane bud 1~
2mm growing point is cultivated and the sugared No. 60 breeding bottle seedlings in disease-free Guangdong for obtaining, access promoting root growth culture medium: 1/2MS+ sucrose
50000mg/L+IBA 2.5mg/L+NAA 1.5mg/L+ paclobutrazol 1.5mg/L, intensity of illumination 2000Lux, daily light application time
16 hours, 28 DEG C of temperature.Visible sugar-cane tissue culture seedlings grow the root system that color is whiter, healthy after promoting root growth culture 25 days.
2) pretreatment before rooted seedling transport: by false stem high Guangdong the about 3~5cm sugar 60 for being tamed hardening take root bottle seedling from
It takes out in culture bottle, the culture medium on seedling is cleaned up with clear water, cuts off the leaf of half, downward one end of long root, put
It is placed in the one layer of pretreated acrylamide and acrylic acid salt cross-linked copolymer plastic box that tiled.Pretreated acrylamide-
Acrylates cross-linked copolymer is: the sodium benzoate of 1.5g/L being added in clear water, after being completely dissolved, pH value is adjusted to 3.5, by matter
It measures than the ratio of 1:70 that the acrylamide and acrylic acid salt cross-linked copolymer and sodium benzoate that particle diameter is 1.5~2.0mm is more molten
Liquid mixing, impregnates 120min.Top one layer of pretreated acrylamide and acrylic acid of very thin lid after seedling is piled one layer in seedling
Salt cross-linked copolymer, then puts one layer of seedling again, and Miao Shangzai is put one layer of acrylamide and acrylic acid salt cross-linked copolymer, finally moulded
The top of magazine covers one layer of plastic film for having air hole again.The volume of the seedling transported after handling in this way is original tape glass
0.26 times when culture bottle, 0.21 times when weight is original tape glass culture bottle, transportation cost reduces 75%.
3) outdoor temporary planting: sugared No. 60 rooted seedlings in Guangdong are taken out from acrylamide and acrylic acid salt cross-linked copolymer, with hand point
At containing 1~4 plant of Cong Miao with root seedling, impregnated after ten minutes with 50% carbendazol wettable powder, 1000 times of liquid, by seedling vacation
It plants in the matrix that loam+bagasse+filter mud is by volume 3:1:1.Shading net is covered within 7 days after temporary planting, daily sooner or later with flower
It spills and pours a water, every the 10 days thin fertilizer of Shi Yici after 25 days seedlings start to turn green, and leaf-cutting promotees to strengthen.The survival rate being planted in this way
95% or more average out to, as shown in Fig. 7.
By practice have shown that acrylamide and acrylic acid salt cross-linked copolymer and the sodium benzoate of suitable concentration are used cooperatively,
Good protective effect can be provided to sugar-cane tissue culture seedlings, the temporary planting after substantially increasing sugar-cane tissue culture seedlings long-distance transport survives
Rate.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
Limitations on the scope of the patent of the present invention therefore cannot be interpreted as.It should be pointed out that for those of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to guarantor of the invention
Protect range.
Claims (10)
1. a kind of transportation resources of sugarcane tissue-culture rooted seedling, comprising the following steps: the acquisition of sugarcane tissue-culture rooted seedling, rooted seedling fortune
Pretreatment, transport, outdoor temporary planting before defeated；It is characterized by: pretreatment is handled to use through antimicrobial fluid before the rooted seedling transport
Protection matrix of the acrylamide and acrylic acid salt cross-linked copolymer as sugarcane tissue-culture rooted seedling, to sugarcane tissue-culture rooted seedling into
Row protection.
2. according to right want 1 described in transportation resources, which is characterized in that pretreated concrete operations before the rooted seedling is transported
Are as follows: the sugarcane tissue-culture rooted seedling through taming hardening is taken out out of culture bottle, culture medium on seedling is eluted with water, cuts off part leaf
Son, downward by one end of long root, slant setting are crosslinked altogether in the acrylamide and acrylic acid salt that one layer is handled through antimicrobial fluid that tiled
In homopolymer plastics case, taken root bottle seedling base portion with acrylamide and acrylic acid salt cross-linked copolymer package sugarcane, above the plastic box
One layer of plastic film with air hole of covering.
3. transportation resources according to claim 2, which is characterized in that the acrylamide and acrylic acid salt cross-linked copolymer
Partial size be 1.5~2.0mm.
4. transportation resources according to claim 2, which is characterized in that the antimicrobial fluid is PhCOONa solution.
5. transportation resources according to claim 4, which is characterized in that using the nitrile-acrylamide-acrylic acid handled through antimicrobial fluid
The concrete operations of hydrochlorate cross-linked copolymer are as follows: in water be added 0.5~3g/L sodium benzoate, until completely dissolved, adjust to
PH3.0~4.0, the ratio of 1:50~100 is acrylamide and acrylic acid salt cross-linked copolymer and PhCOONa solution in mass ratio
60min is impregnated in mixing.
6. according to any transportation resources of claim 2~5, which is characterized in that the sugarcane tissue-culture rooted seedling was placed
The number of plies is 2~5 layers.
7. the method according to claim 1, wherein the concrete operations of the acquisition of the sugarcane tissue-culture rooted seedling
Are as follows: the disease-free sugarcane breeding bottle seedling that sugarcane sugarcane bud 1~2mm growing point is cultivated and obtained is cut, is accessed in promoting root growth culture medium
It is cultivated, promoting root growth culture to sugar-cane tissue culture seedlings grows the root system of white health.
8. transportation resources according to claim 7, which is characterized in that the condition of culture are as follows: intensity of illumination 2000~
2500Lux, daily light application time 10~16 hours, are cultivated 10~30 days by 25~30 DEG C of temperature；The formula of the promoting root growth culture medium
Are as follows: in 1/2MS culture medium be added 50000mg/L sucrose, 1.0~4.0mg/L indolebutyric acid, 0.5~2mg/L methyl α-naphthyl acetate, 1~
3mg/L paclobutrazol.
9. transportation resources according to claim 1, which is characterized in that the concrete operations of the outdoor temporary planting are as follows: by sugarcane
Rooted seedling is taken out from acrylamide and acrylic acid salt cross-linked copolymer, is divided into containing 1~4 plant of Cong Miao with root seedling, with more bacterium
Clever solution impregnates 5~it is planted after ten minutes, every 10 days or so the thin fertilizer of Shi Yici after 20~25 days seedlings start to turn green,
And leaf-cutting promotees to strengthen.
10. according to right required 9 described in transportation resources, which is characterized in that 50% carbendazim of carbendazim solution is wettable
Property 800 to 1000 times of solution of pulvis.
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