CN110476809B - Transportation method of sugarcane tissue culture rooted seedlings - Google Patents
Transportation method of sugarcane tissue culture rooted seedlings Download PDFInfo
- Publication number
- CN110476809B CN110476809B CN201910757981.7A CN201910757981A CN110476809B CN 110476809 B CN110476809 B CN 110476809B CN 201910757981 A CN201910757981 A CN 201910757981A CN 110476809 B CN110476809 B CN 110476809B
- Authority
- CN
- China
- Prior art keywords
- seedlings
- sugarcane
- tissue culture
- culture
- transportation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/55—Sugar cane
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Abstract
The invention discloses a transportation method of tissue-cultured rooted seedlings of sugarcane, which comprises the following steps: obtaining tissue culture rooted seedlings of sugarcane, pretreating the rooted seedlings before transportation, and temporarily planting the rooted seedlings outdoors; during transportation, the acrylamide-acrylate crosslinked copolymer treated by the sodium benzoate is used as a protective matrix, the transportation cost of the sugarcane tissue culture rooted seedlings can be greatly reduced and the survival rate of the temporary planting can be improved by adopting the transportation method of the sugarcane tissue culture rooted seedlings, the acrylamide-acrylate crosslinked copolymer is matched with the sodium benzoate with proper concentration for use, a good protective effect can be provided for the sugarcane tissue culture seedlings, and the survival rate of the temporary planting after the sugarcane tissue culture seedlings are transported for a long distance is greatly improved.
Description
Technical Field
The invention belongs to the technical field of plant tissue culture, and particularly relates to a transportation method of sugarcane tissue culture rooted seedlings.
Background
The sugarcane tissue culture is an efficient propagation technology, and the sugarcane stem tip meristem culture is an effective way for obtaining disease-free seedlings. However, the production base of disease-free sugarcane seedlings is often not in the same area or city as the farm or sugarcane field needing temporary planting and planting, so that the disease-free sugarcane tissue culture rooted seedlings need to be transported from the production base to the farm or sugarcane field needing temporary planting and planting. The seedlings are transported in culture bottles, although the damage to the seedlings is small, the volume and the weight of the seedlings in the bottles to be transported are greatly increased, and the transportation cost is increased. If the culture bottle is removed and only the tissue culture rooted seedlings are transported, although the transportation cost can be reduced, the seedlings can die due to water shortage, oxygen deficiency or mechanical damage in the transportation and storage processes, and therefore the survival rate of the temporary planting is reduced. Therefore, a method which can reduce the transportation cost of the sugarcane tissue culture rooted seedlings, effectively protect the seedlings from being damaged in the transportation process, prolong the storage time of the rooted seedlings and improve the temporary planting survival rate of the transported rooted seedlings is urgently needed.
Disclosure of Invention
The invention aims to solve the technical problems that the conventional transportation method of the sugarcane tissue culture rooted seedlings has high cost and low heeling-in survival rate in the transportation process, and provides the transportation method of the sugarcane tissue culture rooted seedlings, which reduces the transportation cost of the sugarcane tissue culture rooted seedlings and improves the heeling-in survival rate.
The purpose of the invention is realized by the following technical scheme:
the method for transporting the tissue culture rooted seedlings of the sugarcane comprises the following steps: obtaining tissue culture rooted seedlings of sugarcane, pretreating and transporting the rooted seedlings before transportation, and temporarily planting the rooted seedlings outdoors; the pretreatment before the transportation of the rooted seedlings is to protect the sugarcane tissue culture rooted seedlings by adopting an acrylamide-acrylate crosslinked copolymer treated by an antibacterial solution as a protective matrix of the sugarcane tissue culture rooted seedlings.
Further, the pretreatment before the transportation of the rooted seedlings comprises the following specific operations: taking the domesticated and acclimated sugarcane tissue culture rooted seedlings out of the culture bottle, cleaning the culture medium on the seedlings with clear water, cutting off half leaves, placing the seedlings with one end facing downwards in an acrylamide-acrylate cross-linked copolymer plastic box which is paved with a layer of antibacterial liquid for treatment in an inclined way, wrapping the base parts of the sugarcane rooted seedlings in the bottle with the acrylamide-acrylate cross-linked copolymer, and covering a layer of plastic film with air holes above the plastic box. Because the sugarcane tissue culture seedlings are cluster seedlings, the growing points are lower, almost all the sugarcane tissue culture seedlings are concentrated at the base part, and all tillers grow out of the base part.
Preferably, the particle size of the acrylamide-acrylate crosslinked copolymer is 1.5-2.0 mm. Because the diameter of the pseudostem of the sugarcane tissue culture seedling is generally about 1-2 mm, the height of the plant is about 4-8 cm, the plant is thin and easy to break, and the sugarcane tissue culture seedling does not lack oxygen in the transportation process, and the particle size is the particle size most suitable for the sugarcane tissue culture seedling.
Preferably, the antibacterial liquid is a sodium benzoate solution.
Further, the concrete operation of adopting the acrylamide-acrylate crosslinked copolymer treated by the antibacterial solution is as follows: adding 0.5-3 g/L sodium benzoate into water, after completely dissolving, adjusting the pH value to 3.0-4.0, and mixing the materials in a mass ratio of 1: mixing the acrylamide-acrylate crosslinked copolymer with a sodium benzoate solution in a proportion of 50-100, and soaking for 60 min.
Preferably, the number of the layers for placing the sugarcane tissue culture rooted seedlings is 2-5.
More preferably, the number of the layers for placing the sugarcane tissue culture rooted seedlings is 2-3.
Further, the specific operation of obtaining the sugarcane tissue culture rooted seedlings is as follows: cutting a 1-2 mm growing point of sugarcane buds to culture to obtain a disease-free sugarcane propagation bottle seedling, inoculating the disease-free sugarcane propagation bottle seedling into a root promoting culture medium to culture, and performing root promoting culture until a white and healthy root system grows out of the sugarcane tissue culture seedling.
Preferably, the culture conditions are: the illumination intensity is 2000-2500 Lux, the illumination time is 10-16 hours per day, the temperature is 25-30 ℃, and the culture is carried out for 10-30 days; the formula of the root promoting culture medium is as follows: 1/2MS culture medium is added with 50000mg/L sucrose, 1.0-4.0 mg/L indolebutyric acid, 0.5-2 mg/L naphthylacetic acid and 1-3 mg/L paclobutrazol.
Further, the specific operation of the outdoor temporary planting is as follows: taking the sugarcane rooting seedlings out of the acrylamide-acrylate cross-linked copolymer, dividing the sugarcane rooting seedlings into clumpy seedlings containing 1-4 seedlings with roots, soaking the clumpy seedlings in a carbendazim solution for 5-10 minutes, heeling in, applying a thin fertilizer every 10 days after the seedlings turn green after 20-25 days, and shearing leaves to promote the growth of the seedlings.
Preferably, the carbendazim solution is a 800-1000 times solution of 50% carbendazim wettable powder.
The invention has the beneficial effects that:
1. provides a method for reducing the transportation cost of tissue culture rooted seedlings of sugarcane and improving the survival rate of heeling-in, obtains healthy tissue culture seedlings of sugarcane with good growth and healthy and developed root systems, and utilizes the bacteriostasis of a preservative, the good moisture retention of acrylamide-acrylate cross-linked copolymer, the buffering performance of acrylamide-acrylate cross-linked copolymer particles on mechanical compression and the air permeability of gaps among the acrylamide-acrylate cross-linked copolymer particles in the process of transporting the healthy tissue culture rooted seedlings to farms or sugarcane fields needing heeling-in and planting, so that the tissue culture rooted seedlings of sugarcane are prevented from dying due to water shortage, oxygen deficiency, bacterial infection and mechanical damage in the transportation process, the root rot condition is reduced, the storage time of the rooted seedlings is prolonged, the heeling-in survival rate of the transported seedlings is improved, and the transportation volume and weight of the rooted seedlings are effectively reduced, thereby reducing the transportation cost and improving the powerful guarantee for the popularization of the disease-free seedlings of the sugarcane tissue culture. The invention is suitable for being popularized and used by sugarcane scientific research institutions, sugarcane healthy seedling tissue culture factories and the like.
2. The sugarcane tissue culture seedlings are clustered seedlings, the growing points are low, and almost all the seedlings are concentrated at the base. Therefore, if the base part is directly soaked in water or a fresh-keeping solution in the long-distance transportation process, the mechanical damage of the base part of the sugarcane tissue culture seedling caused by oxygen deficiency or mutual overstock friction can be easily caused, and the survival rate of the temporary planting is greatly reduced. If the sugarcane rooting tissue culture seedling strip culture bottle is used for long-distance transportation, although the seedling is well protected, the transportation volume and weight are greatly increased, so that the transportation convenience is influenced, and the transportation cost is increased.
Drawings
FIG. 1 shows the situation that the workers divide the seedlings after centralizing the sugarcane root-promoting tissue culture seedlings in the transport box on the spot.
FIG. 2 shows the growth of tissue-cultured sugarcane seedlings.
FIG. 3 survival of the sugarcane tissue culture seedlings of the pretreatment group in example 1 after 20 days of temporary planting.
FIG. 4 shows the survival of the sugarcane tissue culture seedlings of the first control group in example 1 after 20 days of temporary planting.
FIG. 5 survival of sugarcane tissue culture seedlings of group one after 20 days of temporary planting is treated in example 2.
FIG. 6 survival of sugarcane tissue culture seedlings in group two after 20 days of temporary planting is treated in example 2.
Fig. 7 survival of tissue culture seedling No. 60 Yue sugar in example 3 after 20 days of temporary planting.
Detailed Description
The technical solutions of the present invention are further described below with reference to specific examples, but the present invention is not limited to these specific embodiments. The materials, reagents and the like used in the examples are commercially available unless otherwise specified.
The sugarcane tissue culture seedlings are clustered seedlings, the growing points are low, almost all the seedlings are concentrated at the base, and all the tillers grow out of the base. The diameter of the pseudostem of the sugarcane tissue culture seedling is generally about 1-2 mm, the height of the plant is about 4-8 cm, and the plant is thin and easy to break, as shown in the attached figure 2.
Example 1
Tissue culture rooted seedlings of 45000 black-peel chewing cane healthy seedlings bred from Guangzhou sugarcane sugar industry research institute are delivered to Shaoguan Yangyuan sugarcane area for temporary planting in 2015 3 months, and the temporary planting is carried out according to the following steps:
1) obtaining the sugarcane tissue culture rooted seedlings: and (2) cutting a 1-2 mm growing point of sugarcane buds subjected to hot water treatment and high-temperature germination acceleration at 40 ℃ to culture to obtain disease-free black-peel sugarcane propagation bottle seedlings, and inoculating the bottle seedlings into a root promoting culture medium: 1/2MS, cane sugar 50000mg/L, IBA 2.0mg/L, NAA 1.0mg/L, paclobutrazol 1.0mg/L, illumination intensity of 2000Lux, 16 hours of illumination time every day and 27 ℃. After 25 days of root promoting culture, the sugarcane tissue culture seedlings can grow white and healthy root systems.
2) Pretreatment before transportation of rooted seedlings: taking out the black-peel sugarcane rooting bottle seedlings with the stems about 3-5 cm high after domestication and hardening from the culture bottle, cleaning the culture medium on the seedlings with clear water, and cutting half of leaves. Treatment of test groups: the roots were placed, with one end facing down, in a plastic box that had been tiled with a layer of pre-treated acrylamide-acrylate cross-linked copolymer. The pretreated acrylamide-acrylate crosslinked copolymer was: adding 1.5g/L sodium benzoate into clear water, completely dissolving, adjusting the pH value to 3.5, and mixing the materials according to a mass ratio of 1: 80, mixing the acrylamide-acrylate crosslinked copolymer with the particle diameter of 1.5-2.0 mm with a sodium benzoate solution, and soaking for 120 min. After the seedlings are fully covered with a layer, a layer of pretreated acrylamide-acrylate cross-linked copolymer is thinly covered on the seedlings, and finally, a plastic film with air holes is covered on the plastic box. Originally, a plastic basket with the length of 60cm, the width of 42cm and the height of 18cm can only be used for loading 96 bottles of root-promoting bottle seedlings, 45000 seedlings are 4500 bottles of root-promoting bottle seedlings, the seedlings need to be loaded with 47 baskets, the weight of each basket filled with tissue culture bottle seedlings is about 25kg, and the weight of the seedlings in bottle connection transportation is about 1175 kg. One pick-up truck can load 12 baskets of seedlings in a transport carriage under the condition of no overload, and 45000 seedlings need to be transported four times. The sum of the oil cost, road cost and driver labor cost of one round trip of each transportation is about 1300 yuan, and the sum of four round trips is 5200 yuan. After treatment, the seedlings can be packed by only 12 baskets, the volume of the seedlings is reduced to 25.5 percent of that of the original culture bottle with glass, the total weight of the seedlings is only about 300kg, and the convenience of transportation is greatly improved. All the seedlings can be delivered to the sugarcane area by a pick-up truck for one time, and the delivery cost is only 1/4 for carrying with bottles. Control group one: adding 1.5g/L sodium benzoate directly with clear water, and adjusting pH to 3.5 after completely dissolving. Directly soaking the root of the rooted sugarcane seedling with a sodium benzoate solution. Control group two: directly transporting with a culture bottle without pretreatment.
3) Outdoor temporary planting: the temporary planting is started formally from the completion of the pretreatment of the black skin sugarcane rooted seedlings to the transportation to the sugarcane area of the Chinese pulsatilla root, the seedlings are stored in a plastic box or a culture bottle for more than 30 hours, and the seedlings transported by the culture bottle still grow well and are green, as shown in the attached figure 1. The seedlings placed in the acrylamide-acrylate cross-linked copolymer are similar to seedlings transported by bottles, the color is green, part of new roots grow into cross-linked particles, and the seedlings directly soaked in the sodium benzoate solution begin to yellow, and no new roots grow out. Taking out the rooted sugarcane seedlings, uniformly dividing the rooted sugarcane seedlings into clumpy seedlings containing 1-4 rooted seedlings by hands, soaking the clumpy seedlings in 1000 times of liquid of 50% carbendazim wettable powder for 10 minutes, and heeling the seedlings in a matrix of loam and filter mud in a volume ratio of 3: 1. Covering a film to preserve water and preserve heat after 10 days after temporary planting, counting the survival rate after the seedlings turn green after 20 days, applying thin fertilizer every 10 days, and cutting leaves to promote the growth of the seedlings. The survival rate of the control group is 96.2 percent, and the growth vigor of the sugarcane seedlings is good. The growth vigor of the sugarcane seedlings in the pretreatment group is similar to that of the sugarcane seedlings in the control group II, and the survival rate reaches 95.6 percent. In the control group, one seedling grows weakly and the survival rate is only 38.2 percent. The addition of the acrylamide-acrylate cross-linked copolymer has a very good protection effect on sugarcane seedlings, and the heeling-in survival rate of the sugarcane tissue culture seedlings after long-distance transportation can be greatly improved. The survival condition of the sugarcane seedlings after being heeled in for 20 days is shown in attached figures 3 and 4.
Example 2
Tissue culture rooted seedlings of 38000 yellow skin chewing cane healthy seedlings bred from Guangzhou sugarcane sugar industry research institute are transported to Shaoguan Yangyuan sugarcane area for temporary planting in 2016 (3 months) and are carried out according to the following steps:
1) obtaining the sugarcane tissue culture rooted seedlings: and (2) cutting a 1-2 mm growing point of sugarcane buds subjected to hot water treatment and high-temperature germination acceleration at 40 ℃ to culture to obtain disease-free yellow-peel sugarcane propagation bottle seedlings, and inoculating the bottle seedlings into a root promoting culture medium: 1/2MS + sucrose 50000mg/L + IBA 2.5mg/L + NAA 1.5mg/L + paclobutrazol 1.2mg/L, illumination intensity 2000Lux, illumination time 16 hours per day, and temperature 27 ℃. After 20 days of root-promoting culture, the sugarcane tissue culture seedlings can grow white and healthy root systems.
2) Pretreatment before transportation of rooted seedlings: taking out the yellow skin sugarcane rooting bottle seedlings with the height of the pseudostem of the domesticated and acclimatized seedlings being about 3-5 cm from the culture bottle, cleaning the culture medium on the seedlings with clear water, and cutting half of leaves. The roots were placed, with one end facing down, in a plastic box that had been tiled with a layer of pre-treated acrylamide-acrylate cross-linked copolymer. The pretreated acrylamide-acrylate crosslinked copolymer was: adding a certain amount of sodium benzoate into clear water, completely dissolving, adjusting the pH value to 3.5, and mixing the materials according to a mass ratio of 1: 80, mixing the acrylamide-acrylate crosslinked copolymer with the particle diameter of 1.5-2.0 mm with a sodium benzoate solution, and soaking for 120 min. After the seedlings are fully covered with a layer, a layer of pretreated acrylamide-acrylate cross-linked copolymer is thinly covered on the seedlings, and finally, a plastic film with air holes is covered on the plastic box. Treatment of test groups: firstly, 1.5g/L of sodium benzoate is added into clear water and then mixed with acrylamide-acrylate cross-linked copolymer. And secondly, adding 5.0g/L of sodium benzoate into clear water, and mixing with the acrylamide-acrylate crosslinked copolymer.
3) Outdoor temporary planting: the temporary planting is carried out from the completion of the pretreatment of the rooted seedlings of the wampee sugarcane to the formal start of the transportation to the region of the pulsatilla chinensis, and the seedlings are stored in a plastic box for more than 24 hours. Taking out the rooted sugarcane seedlings, uniformly dividing the rooted sugarcane seedlings into clumpy seedlings containing 1-4 rooted seedlings by hands, soaking the clumpy seedlings in 1000 times of liquid of 50% carbendazim wettable powder for 10 minutes, and heeling the seedlings in a matrix of loam and filter mud in a volume ratio of 3: 1. Covering a film to preserve water and preserve heat after 10 days after temporary planting, counting the survival rate after the seedlings turn green after 20 days, applying thin fertilizer every 10 days, and cutting leaves to promote the growth of the seedlings. The survival rate of the treated group reaches 95.8 percent, and the seedlings grow vigorously; the two-component activity rate of the treatment component is only 72.3 percent, and the growth vigor is slightly weak, which indicates that the concentration of the sodium benzoate is not suitable to be too high. The survival condition of the sugarcane seedlings after being heeled in for 20 days is shown in attached figures 5 and 6.
Example 3
40000 sugarcane Guangdong sugar No. 60 tissue culture rooted seedlings bred from Guangzhou sugarcane sugar industry research institute in Guangzhou city are delivered to a Yangshuan province-sourced Maoyuan sugar factory for temporary planting in 2017 in 5 months, and the temporary planting is carried out according to the following steps:
1) obtaining the sugarcane tissue culture rooted seedlings: and (2) cutting a growing point of 1-2 mm of sugarcane buds subjected to hot water treatment and high-temperature germination acceleration at 40 ℃ to culture to obtain a disease-free Guangdong sugar No. 60 breeding bottle seedling, and inoculating the seedling into a root promoting culture medium: 1/2MS, cane sugar 50000mg/L, IBA 2.5mg/L, NAA 1.5mg/L, paclobutrazol 1.5mg/L, illumination intensity of 2000Lux, 16 hours of illumination time every day and 28 ℃. After 25 days of root promoting culture, the sugarcane tissue culture seedlings can grow white and healthy root systems.
2) Pretreatment before transportation of rooted seedlings: taking out the acclimatized and acclimatized seedling from a culture bottle, cleaning a culture medium on the seedling by using clear water, cutting off half leaves, placing one end of a long root downwards in a plastic box paved with a layer of pretreated acrylamide-acrylate cross-linked copolymer, wherein the height of the pseudostem of the acclimatized and acclimatized seedling is about 3-5 cm. The pretreated acrylamide-acrylate crosslinked copolymer was: adding 1.5g/L sodium benzoate into clear water, completely dissolving, adjusting the pH value to 3.5, and mixing the materials according to a mass ratio of 1: 70, mixing the acrylamide-acrylate crosslinked copolymer with the particle diameter of 1.5-2.0 mm with a sodium benzoate solution, and soaking for 120 min. After the seedlings are covered with a layer of seedlings, a layer of pretreated acrylamide-acrylate crosslinked copolymer is thinly covered on the seedlings, then a layer of seedlings is covered, a layer of acrylamide-acrylate crosslinked copolymer is further arranged on the seedlings, and finally a layer of plastic film with air holes is covered on the plastic box. The volume of the seedlings transported after the treatment is 0.26 times of that of the original glass culture bottles, the weight of the seedlings is 0.21 times of that of the original glass culture bottles, and the transportation cost is reduced by 75 percent.
3) Outdoor temporary planting: taking out the Guangdong sugar No. 60 rooted seedlings from the acrylamide-acrylate cross-linked copolymer, dividing the rooted seedlings into clumpy seedlings containing 1-4 rooted seedlings by hands, soaking the clumpy seedlings in 1000-fold liquid of 50% carbendazim wettable powder for 10 minutes, and heeling the seedlings in a matrix of loam, bagasse and filter mud in a volume ratio of 3:1: 1. Covering a shading net 7 days after temporary planting, watering with a shower head once in the morning and at night every day, applying thin fertilizer every 10 days after the seedlings turn green for 25 days, and shearing leaves to promote the growth of the seedlings. The survival rate of the temporary planting is more than 95% on average, as shown in figure 7.
Practice shows that the acrylamide-acrylate crosslinked copolymer and sodium benzoate with proper concentration are used together, so that a good protection effect can be provided for the sugarcane tissue culture seedlings, and the survival rate of the temporary planting of the sugarcane tissue culture seedlings after long-distance transportation is greatly improved.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention.
Claims (6)
1. A transportation method of tissue culture rooted seedlings of sugarcane comprises the following steps: obtaining tissue culture rooted seedlings of sugarcane, pretreating and transporting the rooted seedlings before transportation, and temporarily planting the rooted seedlings outdoors; the method is characterized in that: the pretreatment before the transportation of the rooted seedlings is to adopt acrylamide-acrylate crosslinked copolymer treated by an antibacterial solution as a protective matrix of the sugarcane tissue culture rooted seedlings to protect the sugarcane tissue culture rooted seedlings;
the pretreatment before the transportation of the rooted seedlings comprises the following specific operations: taking the domesticated and acclimated sugarcane tissue culture rooted seedlings out of a culture bottle, washing a culture medium on the seedlings with water, cutting off partial leaves, obliquely placing one end of a long root downwards in an acrylamide-acrylate cross-linked copolymer plastic box which is paved with a layer of antibacterial liquid for treatment, wrapping the base parts of the sugarcane rooted seedlings in the rooting bottle with the acrylamide-acrylate cross-linked copolymer, and covering a layer of plastic film with air holes above the plastic box;
the specific operation of the outdoor temporary planting is as follows: taking the sugarcane rooting seedlings out of the acrylamide-acrylate cross-linked copolymer, dividing the sugarcane rooting seedlings into clumpy seedlings containing 1-4 seedlings with roots, soaking the clumpy seedlings in a carbendazim solution for 5-10 minutes, heeling in, applying a thin fertilizer every 10 days after the seedlings turn green after 20-25 days, and shearing leaves to promote the growth of the seedlings;
the particle size of the acrylamide-acrylate crosslinked copolymer is 1.5-2.0 mm;
the antibacterial liquid is a sodium benzoate solution.
2. The transportation method according to claim 1, wherein the specific operation of using the acrylamide-acrylate crosslinked copolymer treated with the antibacterial solution is: adding 0.5-3 g/L sodium benzoate into water, after completely dissolving, adjusting the pH value to 3.0-4.0, and mixing the materials in a mass ratio of 1: mixing the acrylamide-acrylate crosslinked copolymer with a sodium benzoate solution in a proportion of 50-100, and soaking for 60 min.
3. The transportation method according to claim 1 or 2, wherein the number of layers for placing the tissue-cultured sugarcane rooted seedlings is 2-5.
4. The transportation method according to claim 1, wherein the specific operation of obtaining the tissue culture rooted seedlings of the sugarcane is as follows: cutting a 1-2 mm growing point of sugarcane buds to culture to obtain a disease-free sugarcane propagation bottle seedling, inoculating the disease-free sugarcane propagation bottle seedling into a root promoting culture medium to culture, and performing root promoting culture until a white and healthy root system grows out of the sugarcane tissue culture seedling.
5. The transportation method according to claim 4, wherein the culture conditions are: the illumination intensity is 2000-2500 Lux, the illumination time is 10-16 hours per day, the temperature is 25-30 ℃, and the culture is carried out for 10-30 days; the formula of the root promoting culture medium is as follows: 50000mg/L of sucrose, 1.0-4.0 mg/L of indolebutyric acid, 0.5-2 mg/L of naphthylacetic acid, 1-3 mg/L of paclobutrazol and 1/2MS culture medium.
6. The method of transportation according to claim 1, wherein the carbendazim solution is 800 to 1000 times diluted solution of 50% carbendazim wettable powder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910757981.7A CN110476809B (en) | 2019-08-16 | 2019-08-16 | Transportation method of sugarcane tissue culture rooted seedlings |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910757981.7A CN110476809B (en) | 2019-08-16 | 2019-08-16 | Transportation method of sugarcane tissue culture rooted seedlings |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110476809A CN110476809A (en) | 2019-11-22 |
| CN110476809B true CN110476809B (en) | 2021-05-11 |
Family
ID=68549807
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910757981.7A Active CN110476809B (en) | 2019-08-16 | 2019-08-16 | Transportation method of sugarcane tissue culture rooted seedlings |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110476809B (en) |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101023734B (en) * | 2007-04-05 | 2011-06-08 | 石俊雄 | Plant seedling-culturing substrate |
| CN101401549B (en) * | 2008-11-04 | 2011-10-26 | 广州甘蔗糖业研究所 | Once-seedling forming quick propagating method for sugarcane tissue culture |
| CN101803574B (en) * | 2010-04-23 | 2012-02-01 | 广州甘蔗糖业研究所 | Detoxication and tissue culture rapid propagation method of chewing cane axillary buds |
| CN102405837B (en) * | 2011-09-06 | 2013-08-21 | 广西亚热带作物研究所试验站 | Method for transplanting sugarcane tissue culture seedlings to fields from low tunnels |
| BR102013007727B1 (en) * | 2013-03-28 | 2022-07-26 | Ctc - Centro De Tecnologia Canavieira S.A. | METHOD FOR PRODUCTION OF SYNTHETIC SUGARCANE SEED, STORAGE OF SYNTHETIC SUGARCANE SEED, PRODUCTION OF SUGARCANE SEEDLINGS AND SYNTHETIC SUGARCANE SEED |
| CN107094623A (en) * | 2017-04-19 | 2017-08-29 | 江苏农林职业技术学院 | A kind of propagation method of sugarcane tissue culture |
| CN107155806A (en) * | 2017-06-05 | 2017-09-15 | 杭州绿风生态环境建设集团有限公司 | A kind of preservation method during large-scale seedling transportation |
| CN107494271A (en) * | 2017-09-29 | 2017-12-22 | 广东省湛江农垦科学研究所 | The method of one seed pod sugarcane detoxication and tissue culture rapid propagation |
| CN108055997A (en) * | 2017-12-05 | 2018-05-22 | 广东省生物工程研究所（广州甘蔗糖业研究所） | A kind of high density temporary planting method of sugar-cane tissue culture seedlings |
| CN107980531A (en) * | 2017-12-25 | 2018-05-04 | 洛阳名力科技开发有限公司 | A kind of method for transplanting of sugarcane tissue-culture nursery |
| CN108781659B (en) * | 2018-06-20 | 2021-06-15 | 湖北省农业科学院粮食作物研究所 | Remote transportation and transplanting method for rice tissue culture seedlings |
-
2019
- 2019-08-16 CN CN201910757981.7A patent/CN110476809B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN110476809A (en) | 2019-11-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102754578B (en) | Method for promoting differentiation of flower buds of cherry | |
| CN106069472B (en) | A method of it is inefficient to solve aged closing Queensland nut garden low yield | |
| CN103548640A (en) | Transplantation method of garden tree | |
| CN106358912A (en) | Apple dwarf self-rooted rootstock high-position dwarf cultivation method | |
| CN107960257A (en) | A kind of gardens arbor method for transplanting | |
| CN103461046A (en) | Astragalus sinicus single-season rice high-yield efficient seed reservation method | |
| CN112753473A (en) | Method for cutting and breeding tea seedlings by inoculating mixed arbuscular mycorrhizal fungi | |
| CN110946034A (en) | Method for improving transplanting survival rate of large osmanthus trees in summer | |
| CN1115095C (en) | Method for cultivating fruit trees in plum line | |
| CN103238469B (en) | Method for breeding new varieties of peach trees | |
| CN104996154A (en) | High-efficiency breeding and transplanting method for Prunus atalantioides | |
| CN104041279B (en) | A kind of Fokienia hodginsii seeding growing seedlings method | |
| CN107439334A (en) | A kind of implantation method of summer high temperature Flos micheliae Albae | |
| CN107197668A (en) | Rhizoma Gastrodiae implantation methods | |
| CN110301237B (en) | Method for cutting and seed reproduction by sugarcane autumn and winter bamboo shoots | |
| CN110476809B (en) | Transportation method of sugarcane tissue culture rooted seedlings | |
| CN108157088A (en) | A kind of method of Rice Cropping | |
| CN112673876A (en) | Method for rapidly forming young tea tree garden | |
| CN107278466A (en) | A kind of method for building up in removable orchard | |
| CN104012356A (en) | Macadamia integrifolia branching-after-topping seedling cultivation method | |
| CN109315198A (en) | A kind of peach plantation breeding method | |
| CN108633897B (en) | Growth regulator for promoting root growth of ratoon rice and application method thereof | |
| CN107047183A (en) | A kind of longan implantation methods | |
| CN112075279B (en) | Method for introducing and cultivating waxberry trees in north | |
| CN109121549A (en) | A method of rhizoma polygonati is improved without bud rhizome germination rate |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP01 | Change in the name or title of a patent holder | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 510316, 10, pomegranate Road, Guangzhou, Guangdong, Haizhuqu District Patentee after: Institute of bioengineering, Guangdong Academy of Sciences Address before: 510316, 10, pomegranate Road, Guangzhou, Guangdong, Haizhuqu District Patentee before: GUANGDONG PROVINCIAL BIOENGINEERING INSTITUTE (GUANGZHOU SUGARCANE INDUSTRY RESEARCH INSTITUTE) |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20220728 Address after: No.10, shiliugang Road, Haizhu District, Guangzhou City, Guangdong Province 510000 Patentee after: Nanfan Seed Industry Research Institute Guangdong Academy of Sciences Address before: 510316, 10, pomegranate Road, Guangzhou, Guangdong, Haizhuqu District Patentee before: Institute of bioengineering, Guangdong Academy of Sciences |