CN108781659A - A kind of rice tissue-cultured seedling long distance transportation and method for transplanting - Google Patents
A kind of rice tissue-cultured seedling long distance transportation and method for transplanting Download PDFInfo
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- CN108781659A CN108781659A CN201810634302.2A CN201810634302A CN108781659A CN 108781659 A CN108781659 A CN 108781659A CN 201810634302 A CN201810634302 A CN 201810634302A CN 108781659 A CN108781659 A CN 108781659A
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- 241000209094 Oryza Species 0.000 claims abstract description 44
- 239000008399 tap water Substances 0.000 claims abstract description 12
- 235000020679 tap water Nutrition 0.000 claims abstract description 12
- 238000004806 packaging method and process Methods 0.000 claims abstract description 11
- 238000002054 transplantation Methods 0.000 claims abstract description 11
- 238000012549 training Methods 0.000 claims abstract description 10
- 208000027418 Wounds and injury Diseases 0.000 claims abstract description 9
- 230000006378 damage Effects 0.000 claims abstract description 9
- 208000014674 injury Diseases 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 44
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- 238000001035 drying Methods 0.000 claims description 5
- 230000000694 effects Effects 0.000 claims description 5
- 230000002262 irrigation Effects 0.000 claims description 5
- 238000003973 irrigation Methods 0.000 claims description 5
- 238000009736 wetting Methods 0.000 claims description 5
- 241001237160 Kallima inachus Species 0.000 claims description 3
- 238000004061 bleaching Methods 0.000 claims description 3
- 239000013039 cover film Substances 0.000 claims description 3
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- 238000009413 insulation Methods 0.000 claims description 3
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- 235000019322 gelatine Nutrition 0.000 claims description 2
- 235000011852 gelatine desserts Nutrition 0.000 claims description 2
- 238000009738 saturating Methods 0.000 claims description 2
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- 238000009395 breeding Methods 0.000 abstract description 8
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- ZKQDCIXGCQPQNV-UHFFFAOYSA-N Calcium hypochlorite Chemical compound [Ca+2].Cl[O-].Cl[O-] ZKQDCIXGCQPQNV-UHFFFAOYSA-N 0.000 abstract description 4
- 239000007844 bleaching agent Substances 0.000 abstract description 4
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- 230000009261 transgenic effect Effects 0.000 description 4
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- 240000008467 Oryza sativa Japonica Group Species 0.000 description 2
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C11/00—Transplanting machines
- A01C11/003—Transplanting machines for aquatic plants; for planting underwater, e.g. rice
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
- A01G22/22—Rice
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65B—MACHINES, APPARATUS OR DEVICES FOR, OR METHODS OF, PACKAGING ARTICLES OR MATERIALS; UNPACKING
- B65B25/00—Packaging other articles presenting special problems
- B65B25/02—Packaging agricultural or horticultural products
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Botany (AREA)
- Soil Sciences (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Agronomy & Crop Science (AREA)
- Mechanical Engineering (AREA)
- Cultivation Of Plants (AREA)
- Packging For Living Organisms, Food Or Medicinal Products That Are Sensitive To Environmental Conditiond (AREA)
Abstract
The invention discloses a kind of rice tissue-cultured seedling long distance transportation and method for transplanting, it is related to the method for seedling transport and transplanting in agricultural technology field.This method includes：1. training tissue culture seedling；2. the packaging of tissue-cultured seedling；3. the transport of tissue-cultured seedling；4. tissue-cultured seedling receives；5. being managed after transplanting and cultivation；Seedling after precision management transplanting to after surviving by normal procedure management completely.The present invention effectively reduces injury of the bleaching powder to tissue-cultured seedling in tap water using the tap water hardening for standing 1~2d；Hardening is transplanted after growing new root, ensure that the survival rate of rice tissue culture transplantation of seedlings；It is capped sunshade net and film after transplanting, is effectively protected the injury that tissue culture transplantation of seedlings initial stage natural environment generates it, further increases seedling percent；The application of this method may advantageously facilitate the strange land transplanting of rice tissue-cultured seedling, accelerates scientific research process, shortens the scientific research of rice relevant rudimentary and breeding cycle.
Description
Technical field
The invention belongs to the methods of seedling transport and transplanting in agricultural technology field, and in particular to a kind of rice tissue-cultured seedling is remote
Distance transport and method for transplanting.
Background technology
Plant tissue culture technique refer under the environmental condition of sterile and manual control, it is right using culture medium appropriate
In vitro plant organ, tissue, cell or protoplast is cultivated, and the technology of its regenerative cell or intact plant is made.Plant
The research of tissue cultures can trace back to early in the twentieth century, be developed rapidly since nearly over half a century, it has also become biology
One important technology of engineering, the effect played in fundamental research and production practices are growing day by day.Tissue culture technique
Anther Culture, genetic transformation, somaclonal variation, micropropagation of plants, somatic embryo are mainly used in production
The foundation etc. of generation and artificial seed, the cryopreservation of histocyte culture and Germplasm Bank.
It is Anther Culture and genetic transfoumation that the tissue culture technique of rice, which is applied most commonly used,.
The main advantage of Anther Culture show as quickly homozygous desirable genes, shorten breeding cycle, improve efficiency of selection and
Accelerate effective character transfer etc..Anther Culture is combined with traditional breeding technology, has been played in rice breeding and scientific research huge
Effect, is mainly manifested in：Rice Germplasm Resources innovation is directly carried out using Anther culture breeding；It is conventional that Anther Culture is applied to rice
Breeding, such as to offspring F1Group carries out reselection after colored training, can greatly shorten breeding cycle；Anther Culture is applied to rice
Crossbreeding, can by the quick homozygous subspecies indica and japonica hybrid advantage of flower training technology, polymerization sterile gene, wide affinity base because, it is extensive
Multiple genes and other beneficial genes cultivate excellent sterile system and wide-congenial restoring line etc..
Since 1988 obtain first transgenic rice plant in japonica rice variety, Transgenic Rice is ground on rice basis
Study carefully and application study in terms of all carried out a large amount of work, be related to the various aspects of rice scientific research and at all levels, be rice
Scientific research is made that huge contribution.Transgenic technology be unable to do without genetic transformation, and tissue cultures are the most important rings of genetic transformation
Section.
Tissue cultures are Anther Culture and the technical foundation of genetic transformation.Due to the particularity of tissue cultures, generation is again
Raw seedling, that is, tissue-cultured seedling is growth and development under the light in human controllable, temperature, wet stabilization and aseptic condition, to carry out follow-up study and
Using needing tissue-cultured seedling being transplanted in natural environment.If not reinforcing the field management after the domestication and transplanting before transplanting, group
The survival rate of training seedling is difficult to be protected.In addition, to further speed up correlative study process and shortening the test period, Anther Culture
The case where being transplanted with the tissue-cultured seedling strange land obtained after genetic transformation can be more and more common, and field-transplanting can better ensure that plant
The growth conditions and subsequent test effect of strain, ensure better setting percentage etc..
The long distance transportation of rice tissue-cultured seedling and transplanting have between same climatic province（Such as Wuhan-Hangzhou）Transport move
It plants, between Different climate region（Such as Wuhan-Hainan）Transport transplanting, be usually relatively suitable in weather between same climatic province
Suitable season transport transplanting, and the transport transplanting between different climatic provinces further relates to the larger situation of the temperature difference, such as Wuhan-Hainan
Between transport transplanting be usually to carry out in winter, Wuhan temperature is very low, and Hainan temperature is suitable, before this is related to transporting packaging and
The case where transport tissue-cultured seedling early period heat preservation avoids enduring cold.Management after hardening and transplanting is even more to ensure the crucial ring that tissue-cultured seedling survives
Section.
Invention content
The purpose of the present invention is that solution the problems of in the prior art, and it is remote to provide a kind of rice tissue-cultured seedling
Transport and method for transplanting.
The object of the present invention is achieved like this.
Specifically, this method includes the following steps：
1. training tissue culture seedling
Tissue-cultured seedling grows into sturdy enough in the medium, when length 10cm or more, opens culture bottle cap, adds water on culture medium
Then 1cm, hardening 3d clean up root culture medium, tissue-cultured seedling yellow leaf and dead leaf are cut off with scissors, are continued to support seedling 3 with water
Culture bottle is placed heliogreenhouse or illumination box, temperature control by~5d until growing the new root of 1cm or so during hardening
At 25 ± 3 DEG C；
2. the packaging of tissue-cultured seedling
Prepare appropriate warm water, temperature is no more than 35 DEG C, ready gauze is soaked with warm water, squeezes out extra moisture, will be wet
Gauze is paved into bilayer on the table, and tissue-cultured seedling is placed in gauze top, by gauze lower folded encase tissue-cultured seedling root and
Gauze is laterally rolled, so that tissue-cultured seedling root and stem is wrapped in wet gauze, the tissue-cultured seedling that will tentatively wrap rapidly by stem
It is put into freshness protection package, blade exposes freshness protection package, loosely touch freshness protection package, ensures that the tissue-cultured seedling of gauze wrapped is not easy to drop out；
The tissue-cultured seedling wrapped is placed in bubble chamber rapidly by the bubble chamber for selecting suitable size, and root is just being put downward, with saturating
Gelatin seals chest, and bubble chamber is tied with strap, reserves portable part；Or bubble chamber is packed into other suitable handbags；
3. the transport of tissue-cultured seedling
Long distance transportation is carried out by the way of air transportation, railway transportation or highway transportation, haulage time should not surpass after vanning
12 hours are spent, and should not be consigned；
4. tissue-cultured seedling receives
Transport arrives in 24 hours latter, and tissue-cultured seedling is transplanted in preprepared rice seedling bed, cannot such as be transplanted immediately,
Tissue-cultured seedling after transport is arrived at, which is opened, to be taken out, and is placed in the basin or bucket of dress water, ensures that root is impregnated in water,
Relative loose between tissue-cultured seedling, should not be crowded, in case seedling yellow leaf, is placed in shady place of the temperature at 25 ± 3 DEG C；
5. being managed after transplanting and cultivation
According to the requirement of the quantity of the seedling to be transplanted and watery ricenursery, the rice seedling bed of suitable size is sorted out in advance, and rice seedling bed mud is kept to melt
Smooth, draining is smooth；By the tissue culture transplantation of seedlings for growing new root to rice seedling bed, sunshade net is covered；If daily mean temperature is low when transplanting
In 22 DEG C, sunshade net will cover film further below；Daily mean temperature should not transplant when being less than 15 DEG C；With alternation of wetting and drying intermittent irrigation
Method Guan Shui, water row after to mend shallow water in a few days, preceding water does not see below water, hestening rooting；General tissue-cultured seedling tames 15 in field
It or so, after tissue-cultured seedling after field survives completely normal management.
The present invention has the advantages that：
1. using the tap water hardening for standing 1~2d, injury of the bleaching powder to tissue-cultured seedling in tap water is effectively reduced；
2. hardening is transplanted after growing new root, the ability that tissue-cultured seedling adapts to transplanting environment is enhanced, ensure that rice tissue culture transplantation of seedlings
Survival rate；
3. capping sunshade net and film, are effectively protected the injury that tissue culture transplantation of seedlings initial stage natural environment generates it after transplanting,
Further increase seedling percent；
4. the application of this method may advantageously facilitate the strange land transplanting of rice tissue-cultured seedling, accelerates scientific research process, shorten rice dependency basis
Plinth scientific research and breeding cycle.
Specific implementation mode
It is described in detail with reference to embodiment：
One, method
1. training tissue culture seedling
The water is the tap water for standing 1~2d, stands the bleaching meal component for being used for reducing or remove tap water to reduce
Injury of the water to tissue-cultured seedling.
2. the packaging of tissue-cultured seedling
The gauze wrapped cannot be too tight；
Tissue-cultured seedling can select subpackage to pack when too many；
Water on gauze more retains as far as possible but is unable to flowing water；
Packaging action is fast, ensures the temperature residing for tissue-cultured seedling；
The bubble chamber depth of foam is 1.5~3cm, ensures its heat insulation effect；
Ensure that tissue-cultured seedling is uprightly just being put after vanning.
3. the transport of tissue-cultured seedling
The transport is long distance transportation, and the long distance transportation is that haulage time is more than or equal to 4 hours or transportation range is more than
Transport equal to 1000 kilometers；
The means of transportation is using efficiently means of transportation, i.e. air transportation, railway transportation or highway transportation；
The transport should not use delivery, in order to avoid damage seedling.
4. tissue-cultured seedling receives
The water is the tap water for standing 1~2d, stands the bleaching meal component for being used for reducing or remove tap water to reduce
Injury of the water to tissue-cultured seedling.
5. being managed after transplanting and cultivation
The transplanting refers both to field-transplanting, does not include the transplanting of phjytotron；
The rice seedling bed need to arrange in advance, make to melt mud and settle, in order to avoid tissue-cultured seedling sinks after planting with mud, influence seedling and survive；
Sunshade net is covered in time after the tissue culture transplantation of seedlings, in case strong illumination dries up seedling；
If daily mean temperature is less than 22 DEG C when the transplanting, film thermal insulating is covered below sunshade net, temperature is suitably beaten when ging up
Film ventilation is opened to prevent the excessively high burn seedlings of temperature；
Daily mean temperature should not be transplanted less than 15 DEG C when the transplanting, in case seedling is freezed to death；
The alternation of wetting and drying intermittent irrigation so as to seedling be not water shortage and can Rapid Rooting survive；
The rice tissue-cultured seedling includes Anther Culture H0For seedling, transgenic paddy rice T0For seedling；
The rice includes long-grained nonglutinous rice and japonica rice.
Two, working mechanism
Tissue-cultured seedling is long term growth in sterile culture vessel, is isolated with external environment, is a kind of unique ecological environment；Refining
The purpose of seedling be to improve tissue-cultured seedling to the adaptability of external environmental condition, improve its photosynthetic ability, keep tissue-cultured seedling strong
It is strong, it is finally reached the transplanting survival rate for improving tissue-cultured seedling.
In the present invention, during tap water is placed, bleaching powder contained in water can decompose, volatilize；Use 1~2d of placement
Tap water hardening can effectively reduce injury of the bleaching powder to tissue-cultured seedling；Water planting can make tissue-cultured seedling grow new root during hardening,
There is the stronger ability for absorbing moisture and nutrition after such tissue culture transplantation of seedlings, transplanting survival rate can be effectively improved；Add after transplanting
Lid sunshade net and film are effectively protected the wound that the natural environments such as tissue culture transplantation of seedlings initial stage strong illumination and low temperature generate it
Evil, further increases seedling percent；Correct packaging and transport tissue-cultured seedling, it is ensured that tissue-cultured seedling moisture does not scatter and disappear, vanning and
Ensure that the temperature residing for tissue-cultured seedling is normal also for preferably heat preservation using bubble chamber in transportational process, is unlikely to be frozen
Evil.
Three, embodiment
Experimental method used in following embodiments is conventional method unless otherwise specified.
The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
1, embodiment 1
It to Hainan Lingshui long distance transportation rice tissue-cultured seedling and is transplanted from Wuhan City, Hubei Province；
1. training tissue culture seedling
Tissue-cultured seedling grows into sturdy enough in the medium, when length 10cm or more, opens culture bottle cap, adds water on culture medium
Then 1cm, hardening 3d clean up root culture medium, tissue-cultured seedling yellow leaf and dead leaf are cut off with scissors, are continued to support seedling 3 with water
~5d, until growing the new root of 1cm or so；Culture bottle is placed into heliogreenhouse or illumination box, temperature control during hardening
At 25 ± 3 DEG C；
Tissue-cultured seedling described in the present embodiment is bloom control, and material field number is 17m1221 before flower training, writes a Chinese character in simplified form 1221, pedigree is
R537/ jade needle perfume (or spice)/16Q1303；It is 454 plants that this example, which transplants tissue-cultured seedling quantity,；
2. the packaging of tissue-cultured seedling
Prepare appropriate warm water, temperature is no more than 35 DEG C, ready gauze is soaked with warm water, squeezes out extra moisture；It will be wet
Gauze is paved into bilayer on the table, and tissue-cultured seedling is placed in gauze top, by gauze lower folded encase tissue-cultured seedling root and
Gauze is laterally rolled, so that tissue-cultured seedling root and stem is wrapped in wet gauze, the tissue-cultured seedling that will tentatively wrap rapidly by stem
It is put into freshness protection package, blade exposes freshness protection package；Freshness protection package loosely is touched, ensures that the tissue-cultured seedling of gauze wrapped is not easy to drop out；
Tissue-cultured seedling is divided into 2 packets packaging in the present embodiment.
Select the bubble chamber of suitable size, length × width × height=30cm × 21cm × 26.5cm, depth of foam 2.5cm that will wrap
The tissue-cultured seedling wrapped is placed in bubble chamber rapidly, and root is just being put downward, and chest is sealed with transparent adhesive tape, and it is suitable that bubble chamber is packed into
Handbag in；
3. the transport of tissue-cultured seedling
Long distance transportation is carried out by the way of air transportation, railway transportation or highway transportation, haulage time should not surpass after vanning
12 hours are spent, and should not be consigned.The present embodiment uses air transportation；
4. tissue-cultured seedling receives
Transport arrives in 24 hours latter, and tissue-cultured seedling is transplanted in preprepared rice seedling bed, cannot such as be transplanted immediately,
Tissue-cultured seedling after transport is arrived at, which is opened, to be taken out, and is placed in the basin or bucket of dress water, ensures that root is impregnated in water,
Relative loose between tissue-cultured seedling, should not be crowded, in order to avoid seedling yellow leaf；It is placed in shady place of the temperature at 25 ± 3 DEG C；
It is transplanted in time after tissue-cultured seedling arrives in the present embodiment；
5. being managed after transplanting and cultivation
According to the requirement of the quantity of the seedling to be transplanted and watery ricenursery, the rice seedling bed of suitable size is sorted out in advance, and rice seedling bed mud is kept to melt
Smooth, draining is smooth；By the tissue culture transplantation of seedlings for growing new root to rice seedling bed, sunshade net is covered；If daily mean temperature is low when transplanting
In 22 DEG C, sunshade net will cover film further below；Daily mean temperature should not transplant when being less than 15 DEG C；With alternation of wetting and drying intermittent irrigation
Method Guan Shui, water row after to mend shallow water in a few days, preceding water does not see below water, hestening rooting；General tissue-cultured seedling tames 15 in field
It or so, after tissue-cultured seedling after field survives completely normal management；
The transport transplanting time of tissue-cultured seedling is on January 10th, 2018 in the present embodiment；Finally counted in the present embodiment tissue-cultured seedling at
Situation living, survives 432 plants, survival rate 95.15% altogether.
2, embodiment 2：
Embodiment 2 and the method flow of embodiment 1 are essentially identical, the difference is that directly transport transplanting after hardening 3d；Transportational process
Middle Insulation is not in place, and tissue-cultured seedling is by slight freeze injury in transit；It is not capped and hides in strict accordance with requirement of the present invention
Screened postive is damaged seedling after transplanting by strong solar radiation；
The present embodiment implements the time as on December 26th, 2013, and transport 208 plants of bloom control of transplanting finally survives 90 plants, survival rate
Only 43.27%.
Claims (4)
1. a kind of rice tissue-cultured seedling long distance transportation and method for transplanting, it is characterised in that include the following steps：
1. training tissue culture seedling
Tissue-cultured seedling grows into sturdy enough in the medium, when length 10cm or more, opens culture bottle cap, adds water on culture medium
Then 1cm, hardening 3d clean up root culture medium, tissue-cultured seedling yellow leaf and dead leaf are cut off with scissors, are continued to support seedling 3 with water
Culture bottle is placed heliogreenhouse or illumination box, temperature control by~5d until growing the new root of 1cm or so during hardening
At 25 ± 3 DEG C；
2. the packaging of tissue-cultured seedling
Prepare appropriate warm water, temperature is no more than 35 DEG C, ready gauze is soaked with warm water, squeezes out extra moisture, will be wet
Gauze is paved into bilayer on the table, and tissue-cultured seedling is placed in gauze top, by gauze lower folded encase tissue-cultured seedling root and
Gauze is laterally rolled, so that tissue-cultured seedling root and stem is wrapped in wet gauze, the tissue-cultured seedling that will tentatively wrap rapidly by stem
It is put into freshness protection package, blade exposes freshness protection package, loosely touch freshness protection package, ensures that the tissue-cultured seedling of gauze wrapped is not easy to drop out；
The tissue-cultured seedling wrapped is placed in bubble chamber rapidly by the bubble chamber for selecting suitable size, and root is just being put downward, with saturating
Gelatin seals chest, and bubble chamber is tied with strap, reserves portable part；Or bubble chamber is packed into other suitable handbags；
3. the transport of tissue-cultured seedling
Long distance transportation is carried out by the way of air transportation, railway transportation or highway transportation, haulage time should not surpass after vanning
12 hours are spent, and should not be consigned；
4. tissue-cultured seedling receives
Transport arrives in 24 hours latter, and tissue-cultured seedling is transplanted in preprepared rice seedling bed, cannot such as be transplanted immediately,
Tissue-cultured seedling after transport is arrived at, which is opened, to be taken out, and is placed in the basin or bucket of dress water, ensures that root is impregnated in water,
Relative loose between tissue-cultured seedling, should not be crowded, in case seedling yellow leaf, is placed in shady place of the temperature at 25 ± 3 DEG C；
5. being managed after transplanting and cultivation
According to the requirement of the quantity of the seedling to be transplanted and watery ricenursery, the rice seedling bed of suitable size is sorted out in advance, and rice seedling bed mud is kept to melt
Smooth, draining is smooth；By the tissue culture transplantation of seedlings for growing new root to rice seedling bed, sunshade net is covered；If daily mean temperature is low when transplanting
In 22 DEG C, sunshade net will cover film further below；Daily mean temperature should not transplant when being less than 15 DEG C；With alternation of wetting and drying intermittent irrigation
Method Guan Shui, water row after to mend shallow water in a few days, preceding water does not see below water, hestening rooting；General tissue-cultured seedling tames 15 in field
It or so, after tissue-cultured seedling after field survives completely normal management.
2. a kind of rice tissue-cultured seedling long distance transportation as described in claim 1 and method for transplanting, it is characterised in that：
The step 1. acquisition of young fringe and 4. in tissue-cultured seedling reception：
The water is the tap water for standing 1~2d, stands the bleaching meal component for being used for reducing or remove tap water to reduce
Injury of the water to tissue-cultured seedling.
3. a kind of rice tissue-cultured seedling long distance transportation as described in claim 1 and method for transplanting, it is characterised in that step 2. group
In the packaging for training seedling：
The gauze wrapped cannot be too tight；
Tissue-cultured seedling can select subpackage to pack when too many；
Water on gauze more retains as far as possible but is unable to flowing water；
Packaging action is fast, ensures the temperature residing for tissue-cultured seedling；
The bubble chamber depth of foam is 1.5~3cm, ensures its heat insulation effect；
Ensure that tissue-cultured seedling is uprightly just being put after vanning.
4. a kind of rice tissue-cultured seedling long distance transportation as described in claim 1 and method for transplanting, it is characterised in that 5. step is moved
In planting and being managed after planting：
The transplanting refers both to field-transplanting, does not include the transplanting of phjytotron；
The rice seedling bed need to arrange in advance, make to melt mud and settle, in order to avoid tissue-cultured seedling sinks after planting with mud, influence seedling and survive；
Sunshade net is covered in time after the tissue culture transplantation of seedlings, in case strong illumination dries up seedling；
If daily mean temperature is less than 22 DEG C when the transplanting, film thermal insulating is covered below sunshade net, temperature is suitably beaten when ging up
Film ventilation is opened to prevent the excessively high burn seedlings of temperature；
Daily mean temperature should not be transplanted less than 15 DEG C when the transplanting, in case seedling is freezed to death；
The alternation of wetting and drying intermittent irrigation so as to seedling be not water shortage and can Rapid Rooting survive.
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| CN112715359A (en) * | 2020-12-29 | 2021-04-30 | 漳州悦阳生物科技有限公司 | Preservative and application thereof in transportation and preservation of tissue culture seedlings by taking off culture medium |
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