CN112715359A - Preservative and application thereof in transportation and preservation of tissue culture seedlings by taking off culture medium - Google Patents
Preservative and application thereof in transportation and preservation of tissue culture seedlings by taking off culture medium Download PDFInfo
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- CN112715359A CN112715359A CN202011601221.6A CN202011601221A CN112715359A CN 112715359 A CN112715359 A CN 112715359A CN 202011601221 A CN202011601221 A CN 202011601221A CN 112715359 A CN112715359 A CN 112715359A
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- culture medium
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- 239000001963 growth medium Substances 0.000 title claims abstract description 62
- 239000003755 preservative agent Substances 0.000 title claims description 37
- 230000002335 preservative effect Effects 0.000 title claims description 36
- 238000004321 preservation Methods 0.000 title claims description 16
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 46
- 230000000694 effects Effects 0.000 claims abstract description 41
- 239000000758 substrate Substances 0.000 claims abstract description 18
- 229930006000 Sucrose Natural products 0.000 claims abstract description 17
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 17
- 239000002994 raw material Substances 0.000 claims description 29
- 241000196324 Embryophyta Species 0.000 claims description 14
- 238000000034 method Methods 0.000 claims description 11
- 230000014759 maintenance of location Effects 0.000 claims description 10
- 238000005507 spraying Methods 0.000 claims description 9
- 239000004062 cytokinin Substances 0.000 claims description 8
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 claims description 8
- 238000004659 sterilization and disinfection Methods 0.000 claims description 6
- 238000012546 transfer Methods 0.000 claims description 6
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 claims description 5
- GPXLRLUVLMHHIK-UHFFFAOYSA-N forchlorfenuron Chemical compound C1=NC(Cl)=CC(NC(=O)NC=2C=CC=CC=2)=C1 GPXLRLUVLMHHIK-UHFFFAOYSA-N 0.000 claims description 5
- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 claims description 5
- 229960001669 kinetin Drugs 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 3
- 235000010804 Maranta arundinacea Nutrition 0.000 claims description 3
- 235000012419 Thalia geniculata Nutrition 0.000 claims description 3
- 239000008223 sterile water Substances 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 241000208442 Sarracenia Species 0.000 claims description 2
- 102000018997 Growth Hormone Human genes 0.000 claims 3
- 108010051696 Growth Hormone Proteins 0.000 claims 3
- 239000000122 growth hormone Substances 0.000 claims 3
- 244000269261 Alocasia Species 0.000 claims 1
- 241000722731 Carex Species 0.000 claims 1
- 241000209504 Poaceae Species 0.000 claims 1
- 244000145580 Thalia geniculata Species 0.000 claims 1
- 210000001519 tissue Anatomy 0.000 description 31
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 19
- 229930192334 Auxin Natural products 0.000 description 13
- 239000002363 auxin Substances 0.000 description 13
- 238000012360 testing method Methods 0.000 description 13
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 description 3
- 241001237160 Kallima inachus Species 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 239000003617 indole-3-acetic acid Substances 0.000 description 3
- JTEDVYBZBROSJT-UHFFFAOYSA-N indole-3-butyric acid Chemical compound C1=CC=C2C(CCCC(=O)O)=CNC2=C1 JTEDVYBZBROSJT-UHFFFAOYSA-N 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 244000151018 Maranta arundinacea Species 0.000 description 2
- 239000003337 fertilizer Substances 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 241001083548 Anemone Species 0.000 description 1
- 244000205754 Colocasia esculenta Species 0.000 description 1
- 235000006481 Colocasia esculenta Nutrition 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- 241001514662 Leptospermum Species 0.000 description 1
- 240000002853 Nelumbo nucifera Species 0.000 description 1
- 235000006508 Nelumbo nucifera Nutrition 0.000 description 1
- 235000006510 Nelumbo pentapetala Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 210000001938 protoplast Anatomy 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 210000001082 somatic cell Anatomy 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses an antistaling agent and application thereof in transportation and fresh-keeping of tissue culture seedlings by taking a culture medium as a substrate, and adding white sugar and an activity retaining agent into the substrate to be mixed.
Description
Technical Field
The invention relates to the technical field of preservatives, and particularly relates to a preservative and application thereof in transportation and preservation of tissue culture seedlings by taking off a culture medium.
Background
The plant tissue culture, i.e. plant sterile culture technique, is a discipline which utilizes the isolated organs of plant body, such as root, stem, leaf, stem tip, flower and fruit, etc., tissues (such as cambium, epidermis, cortex, medulla cell and endosperm, etc.) or cells (such as megaspore, microspore and somatic cell, etc.) and protoplast according to the theory that plant cells have totipotency, and can induce callus, adventitious bud and adventitious root under the artificial conditions of sterile and proper artificial culture medium, illumination, temperature, etc., and finally form complete plants.
When present group banks up seedling with earth and carries out shipment transportation, if take the culture medium, then there is the transportation total great, increases the cost of transportation and easily infects the fungus on the way, leads to the realistic problem of pollution loss. If the culture medium is removed for transportation, the weight can be greatly reduced, the transportation cost is reduced, and the pollution loss caused by transportation can also be reduced; however, in the process of transporting the culture medium, the tissue culture seedling is not provided with nutrition, so that the nutrient in the tissue culture seedling is consumed, the probability that the tissue culture seedling has yellow leaves, dry leaves and weakens is increased, and the survival rate of transplantation is further influenced, so that the research and the hardness of the fresh-keeping transportation of the tissue culture seedling on the culture medium is researched, and the method is a topic with positive practical significance.
Disclosure of Invention
Aiming at the condition of the prior art, the invention aims to provide the preservative which is simple to prepare, good in preservation effect, low in cost and convenient to apply, and the application of the preservative in transportation and preservation of tissue culture seedling culture medium.
In order to achieve the technical purpose, the technical scheme adopted by the invention is as follows:
a fresh-keeping agent is prepared by using culture medium as substrate, adding white sugar and activity retention agent into the substrate, and mixing.
As a possible implementation mode, the fertilizer is further added with auxin, the auxin is IAA (indole-3-acetic acid), IBA (indole butyric acid) or NAA (naphthalene acetic acid), and the mass concentration of the auxin in the raw material is 0.5-5 ppm.
Preferably, the culture medium is an MS culture medium, and the mass concentration of the white sugar in the raw materials is 10-30 g/L.
As a preferred embodiment, preferably, the activity maintaining agent is plant cytokinin 6-BA, KT kinetin or CPPU, wherein,
when the activity maintaining agent is plant cytokinin 6-BA, the mass concentration of the activity maintaining agent in the raw material is 0.5-10 ppm;
when the activity maintaining agent is KT kinetin, the mass concentration of the activity maintaining agent in the raw materials is 1-10 ppm;
when the activity maintaining agent is CPPU, the mass concentration of the activity maintaining agent in the raw material is 0.1-1 ppm.
As a preferred implementation choice, preferably, the culture medium is a ready-made MS culture medium, which is prepared by mixing the following components in mass concentration:
as a more preferable implementation choice, preferably, the culture medium is a ready-made MS culture medium, which is prepared by mixing the following components in mass concentration:
based on the technical scheme of the preservative, the invention also provides an application of the preservative in transportation and preservation of tissue culture seedling off culture medium, the application comprises the preservative, and the application comprises the following application steps:
(1) uniformly spraying the preservative on the surface of the tissue culture seedling to obtain a pretreated tissue culture seedling;
(2) and (4) putting the pretreated tissue culture seedlings into a transport container after disinfection and sterilization.
As a better implementation choice, the antistaling agent is preferably packed in a spray container for spraying, and the container is washed by using sterile water after being washed inside and outside alcohol before being packed with the antistaling agent.
Preferably, the transportation container is further sterilized at 115-125 ℃ for 110-130 min.
Preferably, the tissue culture seedling comprises at least one of Heguo taro, tendril-leaved velveteen, pitcher plant, arrowroot, fern, Guanyin lotus, Roughhaired Leptospermum herb and Anemone reticulata.
Based on the application scheme of the preservative, the invention also provides a tissue culture seedling transportation and preservation method, which comprises the application of the preservative in the transportation and preservation of the tissue culture seedling culture medium.
By adopting the technical scheme, compared with the prior art, the invention has the beneficial effects that: the preservative can be used for tissue culture seedlings without a culture medium, and is sprayed on the seedlings without the culture medium in a liquid fertilizer mode by adding the nutrients required by plants and the components for keeping the activity of the plants, so that the nutrients required by the transportation process of the tissue culture seedlings are provided on one hand, the activity of the tissue culture seedlings can be kept in the transportation process on the other hand, and meanwhile, the conditions of withered leaves, yellow leaves and weak growth force are greatly reduced, the transplanting success rate is improved.
Drawings
The invention will be further explained with reference to the drawings and the detailed description below:
FIG. 1 is a picture of the leaf state of a tissue culture seedling in 14 days without preservative spraying in the scheme of the present invention, wherein a region frame with dead leaves and yellow leaves is selected;
FIG. 2 is a picture of the leaf state of a tissue culture seedling sprayed with the preservative in the scheme of the invention at 14 days, wherein the situation of withered leaves and yellow leaves is not seen.
Detailed Description
Example 1
The embodiment provides a preservative which is prepared by taking a culture medium as a substrate, adding white sugar and an activity retention agent into the substrate and mixing.
Wherein the culture medium is an MS culture medium, and the mass concentration of the white sugar in the raw materials is 20 g/L; the activity maintaining agent is plant cytokinin 6-BA, and the mass concentration of the activity maintaining agent in the raw material is 5 ppm.
In addition, the culture medium in this example is a ready-made MS culture medium, which is prepared by mixing the following components in mass concentration:
example 2
The embodiment provides a preservative which is prepared by taking a culture medium as a substrate, adding white sugar and an activity retention agent into the substrate and mixing.
Wherein the culture medium is an MS culture medium, and the mass concentration of the white sugar in the raw materials is 20 g/L; when the activity maintaining agent is KT kinetin, the mass concentration of the activity maintaining agent in the raw material is 5 ppm.
In addition, the culture medium in this example is a ready-made MS culture medium, which is prepared by mixing the following components in mass concentration:
example 3
The embodiment provides a preservative which is prepared by taking a culture medium as a substrate, adding white sugar and an activity retention agent into the substrate and mixing.
Wherein the culture medium is an MS culture medium, and the mass concentration of the white sugar in the raw materials is 20 g/L; the activity retention agent is CPPU, and the mass concentration of the activity retention agent in the raw material is 0.6 ppm.
In addition, the culture medium in this example is a ready-made MS culture medium, which is prepared by mixing the following components in mass concentration:
example 4
The embodiment provides a preservative which is prepared by taking a culture medium as a substrate, adding white sugar and an activity retention agent into the substrate and mixing.
Wherein the culture medium is an MS culture medium, and the mass concentration of the white sugar in the raw materials is 20 g/L; the activity maintaining agent is plant cytokinin 6-BA, and the mass concentration of the activity maintaining agent in the raw material is 5 ppm.
In addition, the raw material is also added with auxin, the auxin is IAA, and the mass concentration of the auxin in the raw material is 2.5 ppm.
The culture medium in the embodiment is a ready-made MS culture medium, and is prepared by mixing the following components in mass concentration:
example 5
The embodiment provides a preservative which is prepared by taking a culture medium as a substrate, adding white sugar and an activity retention agent into the substrate and mixing.
Wherein the culture medium is an MS culture medium, and the mass concentration of the white sugar in the raw materials is 20 g/L; the activity maintaining agent is plant cytokinin 6-BA, and the mass concentration of the activity maintaining agent in the raw material is 5 ppm.
In addition, the raw material is also added with auxin, the auxin is IBA, and the mass concentration of the auxin in the raw material is 2.5 ppm.
The culture medium in the embodiment is a ready-made MS culture medium, and is prepared by mixing the following components in mass concentration:
example 6
The embodiment provides a preservative which is prepared by taking a culture medium as a substrate, adding white sugar and an activity retention agent into the substrate and mixing.
Wherein the culture medium is an MS culture medium, and the mass concentration of the white sugar in the raw materials is 20 g/L; the activity maintaining agent is plant cytokinin 6-BA, and the mass concentration of the activity maintaining agent in the raw material is 5 ppm.
In addition, the raw material is also added with auxin, the auxin is NAA, and the mass concentration of the auxin in the raw material is 2.5 ppm.
The culture medium in the embodiment is a ready-made MS culture medium, and is prepared by mixing the following components in mass concentration:
comparison of tests
The preservative prepared in the embodiments 1 to 6 is sprayed on the tissue culture seedlings as an experimental group, the tissue culture seedlings without the preservative is used as a blank control group, and the preservation capability test is carried out, wherein the treatment steps of the experimental group are as follows:
(1) the preservative is put into a spraying container for spraying application, the container is washed with sterile water after being washed inside and outside alcohol before being put into the preservative, and the preservative is uniformly sprayed and applied to the surface of the tissue culture seedling through the spraying container to obtain a pretreated tissue culture seedling;
(2) placing the pretreated tissue culture seedling into a sterilized and sterilized transfer container, and sterilizing the transfer container at 121 deg.C for 121 min.
The blank control group was processed by the following steps:
placing the tissue culture seedling into a sterilized and sterilized transfer container, and sterilizing the transfer container at 121 deg.C for 121 min.
The method comprises the steps of taking a plastic bag as a transfer container, taking arrowroot tissue culture seedlings as test samples, respectively taking the uniform number of leaves (2 in the test), taking the tissue culture seedlings with similar growth conditions, no dead leaves, healthy appearance and similar color as the test samples, respectively preparing 7 groups of comparative test samples, and 8 tissue culture seedlings in each group.
Wherein, the antistaling agent prepared in the embodiment 1-6 is adopted to process the test samples according to the experimental group processing steps to obtain 6 groups of experimental group test samples, and in addition, the test samples are processed according to the blank control group processing steps to obtain 1 group of blank control group test samples; and (3) storing the 7 groups of test samples in a simulated transportation environment for 14 days, and then counting the dead leaves of the test samples, wherein the obtained results are as follows:
TABLE 1 fresh keeping test results
| Number of days of group | Day 0 | 14 days |
| Example 1 | Has no withered leaf or yellow leaf | 2 dead leaves, 2 yellow leaves, and the rest normal |
| Example 2 | Has no withered leaf or yellow leaf | 2 dead leaves, 3 yellow leaves, and the rest normal |
| Example 3 | Has no withered leaf or yellow leaf | 2 dead leaves, 2 yellow leaves, and the rest normal |
| Example 4 | Has no withered leaf or yellow leaf | 1 dead leaf, 2 yellow leaves, and the rest normal |
| Example 5 | Has no withered leaf or yellow leaf | 0 dead leaf, 2 yellow leaves, and the rest normal |
| Example 6 | Has no withered leaf or yellow leaf | 1 dead leaf, 1 yellow leaf, and the rest normal |
| Blank control example | Has no withered leaf or yellow leaf | 4 dead leaves, 3 yellow leaves, 1 normal |
From the results, the tissue culture seedlings subjected to the preservation treatment by combining the preservative and the corresponding application method have a good preservation effect, and compared with a control example without the preservative, the effect is obvious, and after the auxin is added, the situation of dead leaves is improved, so that the good preservation effect is obtained.
In the implementation of the scheme, the method can be further optimized to avoid dead leaves as much as possible as shown in figure 2, and has a higher practical application value compared with the traditional tissue culture seedling (as shown in figure 1) which is not treated by the preservative.
The foregoing is directed to embodiments of the present invention, and equivalents, modifications, substitutions and variations such as will occur to those skilled in the art, which fall within the scope and spirit of the appended claims.
Claims (10)
1. An antistaling agent is characterized in that: the raw materials are prepared by taking a culture medium as a substrate, adding white sugar and an activity retention agent into the substrate and mixing.
2. An antistaling agent according to claim 1, characterized in that: the growth hormone is also added, the growth hormone is IAA, IBA or NAA, and the mass concentration of the growth hormone in the raw materials is 0.5-5 ppm.
3. An antistaling agent according to claim 1 or 2, characterized in that: the culture medium is an MS culture medium, and the mass concentration of the white sugar in the raw materials is 10-30 g/L.
4. An antistaling agent according to claim 3, characterized in that: the activity maintaining agent is plant cytokinin 6-BA, KT kinetin or CPPU, wherein,
when the activity maintaining agent is plant cytokinin 6-BA, the mass concentration of the activity maintaining agent in the raw material is 0.5-10 ppm;
when the activity maintaining agent is KT kinetin, the mass concentration of the activity maintaining agent in the raw materials is 1-10 ppm;
when the activity maintaining agent is CPPU, the mass concentration of the activity maintaining agent in the raw material is 0.1-1 ppm.
5. An antistaling agent according to claim 4, characterized in that: the culture medium is a ready-made MS culture medium and is prepared by mixing the following components in mass concentration:
6. an antistaling agent according to claim 5, characterized in that: the culture medium is a ready-made MS culture medium and is prepared by mixing the following components in mass concentration:
7. the application of the preservative in the transportation and preservation of the tissue culture seedling culture medium is characterized in that: which comprises the preservative according to any one of claims 1 to 6, and which further comprises the following application steps:
(1) uniformly spraying the preservative on the surface of the tissue culture seedling to obtain a pretreated tissue culture seedling;
(2) and (4) putting the pretreated tissue culture seedlings into a transport container after disinfection and sterilization.
8. The application of the preservative according to claim 7 in transportation and preservation of tissue culture seedlings by taking off culture medium, which is characterized in that: the preservative is filled in a spraying container for spraying application, and before the preservative is filled in the container, the container is washed by alcohol inside and outside and then is washed by sterile water; the transfer container is further subjected to disinfection and sterilization treatment at the temperature of 115-125 ℃ for 110-130 min.
9. The application of the preservative according to claim 7 in transportation and preservation of tissue culture seedlings by taking off culture medium, which is characterized in that: the tissue culture seedling at least comprises more than one of Heguocao, tendril-leaved velveteen, pitcher plant, arrowroot, fern, alocasia, carex deltoidea and reticulate grasses.
10. A method for transporting and preserving tissue culture seedlings is characterized by comprising the following steps: which comprises the application of the preservative of any one of claims 7 to 9 in the transportation and preservation of tissue culture seedlings by taking off culture medium.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011601221.6A CN112715359A (en) | 2020-12-29 | 2020-12-29 | Preservative and application thereof in transportation and preservation of tissue culture seedlings by taking off culture medium |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011601221.6A CN112715359A (en) | 2020-12-29 | 2020-12-29 | Preservative and application thereof in transportation and preservation of tissue culture seedlings by taking off culture medium |
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| Publication Number | Publication Date |
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| CN112715359A true CN112715359A (en) | 2021-04-30 |
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| CN202011601221.6A Pending CN112715359A (en) | 2020-12-29 | 2020-12-29 | Preservative and application thereof in transportation and preservation of tissue culture seedlings by taking off culture medium |
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