CN106888903A - A kind of blueberry tissue culture bare-root seeding field-transplanting method - Google Patents
A kind of blueberry tissue culture bare-root seeding field-transplanting method Download PDFInfo
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- CN106888903A CN106888903A CN201710069205.9A CN201710069205A CN106888903A CN 106888903 A CN106888903 A CN 106888903A CN 201710069205 A CN201710069205 A CN 201710069205A CN 106888903 A CN106888903 A CN 106888903A
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- blueberry
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of blueberry tissue culture bare-root seeding field-transplanting method, comprise the following steps：(1) nursery stock pretreatment；(2) seedbed makes；(3) seedling cave addition substrate preparation；(4) transplant；(5) managed after planting.The present invention uses special root-growing agent and cultivation matrix, individual plant bare-root seeding is directly transplanted to the big Tanaka of ready seedling breeding, survival rate management etc. is improved after cultivation so that immature blueberry tissue culture seedling directly carries out field-transplanting and also can guarantee that survival rate higher heeling in the strong sprout stage without nutritive cube.The present invention is compared with conventional blueberry tissue culture method for transplanting, reduce tissue-cultured seedling pot planting this link, shorten growing-seedling period, extend the nursery stock grown in field time, seedling cost and cost of transportation are reduced, while also solving the problems, such as existing blueberry with pot planting high cost, formality be numerous, root system is weak.
Description
Technical field
The invention belongs to field of plant growing technology, and in particular to a kind of blueberry tissue culture bare-root seeding field-transplanting method.
Background technology
Blueberry (Vaccinium Spp), also known as cowberry, blueberry, belong to Ericaceae (Ericaceae) Vaccinium
(vaccinium) plant, is perennial shrub small berries.Not only nutritive value is high for Blueberry, and with important guarantor
Containing abundant anthocyanidin in strong effect and medical value, particularly fruit, to pre- preventing thrombosis and artery sclerosis, anticancer, resistance
Urinary system infection contamination, prevention heart disease and anti-aging are all acted on good, are described as " 21 century functional health slurry
Queen really in " with " fruit ", and one of big healthy food of the mankind five is classified as by international food and agricultural organization, as new in the world today
One of main fruit kind of emerging cultivation.
Blueberry plantation nursery stock is generally tissue-cultured seedling, in order to improve tissue-cultured seedling transplanting survival rate, according to the situation of current industry, is led to
Often blueberry tissue culture seedling is also needed by least year from the outside sprout-cultivating-bottle culture of culturing room to this time period of field-transplanting
Individual plant pot planting this intermediate link is carried out, this link need to put into substantial amounts of manpower and materials, improve the production of seedling
Cost, moreover, the growth period just corresponding shortening in crop field, so as to delay breeding and promotion rate for good seed.
The content of the invention
Goal of the invention：The technical problem to be solved in the present invention is：A kind of raising blueberry tissue culture bare-root seeding transplant survival is provided
The method of rate, to overcome the later stage to exist when being colonized, pot planting transport inconvenience, high cost, formality be numerous, root system is weak and survival rate
Low problem.
Technical scheme：In order to solve the above technical problems, the present invention is adopted the technical scheme that：
A kind of blueberry tissue culture bare-root seeding field-transplanting method, comprises the steps of：
(1) nursery stock pretreatment：The blueberry tissue culture bottle seedling for breeding for 3~5 generations is cultivated 90~120 in outside sprout-cultivating-bottle rear chamber
My god, continue outside scenery and taken out from basin after 30~45 days, all blades in middle and lower part are extractd, it is then that the root system of whole plant is complete
Portion's special root-growing agent of immersion takes out after 1~2 minute and obtains blueberry tissue culture bare-root seeding to be planted；The temperature ﹤ of the outside scenery
30℃；
(2) seedbed makes：Crop field deep ploughing is digged, soil improvement to pH value is 4.5~5.0 by addition Cosan, is obtained
Blueberry seedbed, sets blueberry seedling cave on the blueberry seedbed；
(3) seedling cave addition substrate preparation：By mushroom slag, biogas residue, pine needle, pine tree class bark wood chip and chicken manure, cow dung, pig manure etc.
The fermentation of animal dung batch mixing pile-turning obtains matrix, then adds insecticide and bactericide, after stirring, from phosphoric acid, ferrous sulfate
The pH value for adjusting matrix is 4.5~5.5, and with covered rearing with plastic film 8~10 days, obtains blueberry seedling cave and add matrix；
(4) transplant：The blueberry seedling cave addition base obtained in step (3) is sprinkled in the blueberry seedling cave obtained in step (2)
Overlying soil after matter, the blueberry seedling obtained in step (1) is placed on the soil layer vertically, surrounding again with soil covering to ground
Face maintains an equal level, then pours root water around the blueberry seedling；
(5) managed after planting：The field management such as the later stage watered, weeding, prevention and control of plant diseases, pest control work.
In step (1), the root-growing agent is the aqueous solution (sterilized water) containing indolebutyric acid (IBA) and Nafusaku, wherein
The concentration of IBA is 0.8~l.0g/L, the mass ratio of IBA and Nafusaku is 18:1~20:1.
In step (1), the intensity of illumination of the indoor culture is 20001x~30001x, cultivation temperature is 22 ± 3 DEG C.
In step (2), the height in the blueberry seedbed is 50~60cm, width is 2.5~3.0m, the blueberry seedling cave
Hill spacing is 20~30cm, line-spacing is 35~40cm, depth is 25~30cm.
In step (3), the parameter of the matrix is as follows：Mass percentage content >=45% of organic matter, matrix particle diameter≤
5mm, matrix unit weight are 0.15~0.50g/cm3、N+P2O5+K2O total nutrients in terms of butt >=1%, EC values≤1.0ms/cm, contain
Water rate≤30%；The insecticide and bactericide are potassium permanganate, when using, are configured to the height of mass percent 0.1~0.5%
Mangaic acid aqueous solutions of potassium, is sprayed onto in matrix, until irrigating.
In step (4), the addition thickness of the blueberry seedling cave addition matrix is 10~15cm；The thickness of the layer is 2
~4cm；The root water is the compound sodium nitrophenolate aqueous solution of mass percent concentration 0.003~0.009%, every plant of blueberry seedling
Pour 1 liter of root water.
In step (5), the watering use water-fertilizer integral administrative skill in being managed after the cultivation, weeding artificial weeding,
The prevention and control of plant diseases, pest control is made between using, installs the biologies such as blacklight trap, physical method preventing and treating blueberry pest and disease damage.
The present invention can shorten growing-seedling period compared with conventional nutraceutical alms bowl nursery, reduce in the middle of production link, reduce nursery into
This, extends the nursery stock grown in field time, improves seedling quality, and can reduce cost of transportation and Cultivate administration of the later stage when planting into
This.Using nursery stock of the invention pretreatment and transplanting method, moisture evaporation and the growth beneficial to blueberry root system can be reduced, so as to carry
The survival rate of blueberry bare-root seeding high.Method for transplanting of the invention is not transplanted by season and limited, and can reduce transplanting by water resource, gas
Time, the influence of geographical environment, and ensure the quality transplanted, reduce investment.
Beneficial effect：The method of the present invention is simple to operation, and not by season transplant limited, can reduce transplanting being provided by water
Source, weather, the influence of geographical environment.
Specific embodiment
According to following embodiments, the present invention may be better understood.However, as it will be easily appreciated by one skilled in the art that real
Apply the content described by example and be merely to illustrate the present invention, without should also without limitation on sheet described in detail in claims
Invention.
The blueberry tissue culture bare-root seeding that the present invention is used is provided by Anhui Province's blueberry Engineering Technical Research Centre.
Embodiment 1
A kind of blueberry tissue culture bare-root seeding field-transplanting method, step is as follows：
(1) nursery stock pretreatment：Breed the blueberry tissue culture bottle seedling in 3 generations after outside sprout-cultivating-bottle, intensity of illumination be 20001x~
30001x, temperature are indoor culture 90 days under conditions of 22 ± 3 DEG C, continue outside scenery after 40 days, are taken out from basin, in excision
The all blades in bottom, are then totally immersed into special root-growing agent 1~2 minute by the root system of whole plant, and special root-growing agent is containing Yin
The aqueous solution (sterilized water) of diindyl butyric acid (IBA) and Nafusaku, the wherein concentration of IBA are the matter of 0.8g/L, IBA and Nafusaku
Amount is than being 18:1, then the whole root system of plant is wrapped up into (10 plants/prick) with preservative film, obtain naked of blueberry tissue culture to be planted
Seedling.
(2) seedbed makes：Crop field deep ploughing is digged, soil improvement to pH value is 4.5~5.0 by addition Cosan, is obtained
Blueberry seedbed, sets blueberry seedling cave on the blueberry seedbed；The height in blueberry seedbed is 50cm, width is 2.5m, the indigo plant
The hill spacing in certain kind of berries seedling cave is 20cm, line-spacing is 40cm, depth is 25~30cm.
(3) seedling cave addition substrate preparation：By mushroom slag, biogas residue, pine needle, pine tree class bark wood chip and chicken manure, cow dung, pig manure etc.
The fermentation of animal dung batch mixing pile-turning obtains matrix, then adds insecticide and bactericide, after stirring, from phosphoric acid, ferrous sulfate
The pH value for adjusting matrix is 4.5~5.5, and with covered rearing with plastic film 8~10 days, obtains blueberry seedling cave and add matrix；Organic matter
Mass percentage content >=45%, matrix particle diameter≤5mm, matrix unit weight be 0.15~0.50g/cm3、N+P2O5+K2O is always supported
Point in terms of butt >=1%, EC values≤1.0ms/cm, moisture content≤30%；The insecticide and bactericide are potassium permanganate, are used
When, the potassium permanganate solution of mass percent 0.1~0.5% is configured to, it is sprayed onto in matrix, until irrigating.
(4) transplant：The self-control matrix that thickness is 10~15cm is first sprinkled in seedling cave before transplanting, covering thickness in matrix again is
The soil layer of 2~4cm, blueberry seedling is placed on the soil layer in planting pit vertically, and nursery stock surrounding is extremely held with soil covering with ground again
Flat, the final depth of planting is covering 2~4cm of seedling root, then root water is poured around nursery stock, and root water is that mass percent is dense
The compound sodium nitrophenolate aqueous solution of degree 0.003~0.009%, every plant of blueberry seedling pours 1 liter of root water.
(5) managed after planting：Later stage proceed by water, weeding, the field management such as prevention and control of plant diseases, pest control work.Water and use water
Fertile integrated management technology, weeding artificial weeding, the prevention and control of plant diseases, pest control is made between using, installs biology, the physics such as blacklight trap
Method prevents and treats blueberry pest and disease damage.
Embodiment 2
A kind of blueberry tissue culture bare-root seeding field-transplanting method, step is as follows：
(1) nursery stock pretreatment：The blueberry tissue culture bottle seedling for breeding for 4 generations is cultivated 110 days in outside sprout-cultivating-bottle rear chamber, continues outdoor
After culture 35 days, taken out from basin, extract all blades in middle and lower part, the root system of whole plant is then totally immersed into special taking root
Agent 1~2 minute, special root-growing agent is the aqueous solution (sterilized water) containing indolebutyric acid (IBA) and Nafusaku, and wherein IBA's is dense
It is 0.9g/L to spend, and the mass ratio of IBA and Nafusaku is 19:1, then by the whole root system of plant with preservative film wrap up (10 plants/
Prick), obtain blueberry tissue culture bare-root seeding to be planted.
(2) seedbed makes：Crop field deep ploughing is digged, soil improvement to pH value is 4.5~5.0 by addition Cosan, is obtained
Blueberry seedbed, sets blueberry seedling cave on the blueberry seedbed；The height in blueberry seedbed is 55cm, width is 2.8m, the indigo plant
The hill spacing in certain kind of berries seedling cave is 25cm, line-spacing is 35cm, depth is 25~30cm.
(3) seedling cave addition substrate preparation：By mushroom slag, biogas residue, pine needle, pine tree class bark wood chip and chicken manure, cow dung, pig manure etc.
The fermentation of animal dung batch mixing pile-turning obtains matrix, then adds insecticide and bactericide, after stirring, from phosphoric acid, ferrous sulfate
The pH value for adjusting matrix is 4.5~5.5, and with covered rearing with plastic film 8~10 days, obtains blueberry seedling cave and add matrix；The base
The parameter of matter is as follows：Mass percentage content >=45% of organic matter, matrix particle diameter≤5mm, matrix unit weight is 0.15~
0.50g/cm3、N+P2O5+K2O total nutrients in terms of butt >=1%, EC values≤1.0ms/cm, moisture content≤30%；The insecticide
It is potassium permanganate with bactericide, when using, is configured to the potassium permanganate solution of mass percent 0.1~0.5%, is sprayed onto base
In matter, until irrigating.
(4) transplant：The self-control matrix that thickness is 10~15cm is first sprinkled in seedling cave before transplanting, covering thickness in matrix again is
The soil layer of 2~4cm, spacing in the rows 25cm, line-spacing 35cm, blueberry seedling is placed on the soil layer in planting pit vertically, and nursery stock surrounding is again
Maintained an equal level to ground with soil covering, the final depth of planting is covering 2~4cm of seedling root, then root water is poured around nursery stock, fixed
Root water is the compound sodium nitrophenolate aqueous solution of mass percent concentration 0.003~0.009%, and every plant of blueberry seedling pours 1 liter of root water.
(5) managed after planting：Later stage proceed by water, weeding, the field management such as prevention and control of plant diseases, pest control work.Water and use water
Fertile integrated management technology, weeding artificial weeding, the prevention and control of plant diseases, pest control is made between using, installs biology, the physics such as blacklight trap
Method prevents and treats blueberry pest and disease damage.
Embodiment 3
A kind of blueberry tissue culture bare-root seeding field-transplanting method, step is as follows：
(1) nursery stock pretreatment：The blueberry tissue culture bottle seedling for breeding for 5 generations is cultivated 120 days in outside sprout-cultivating-bottle rear chamber, continues outdoor
After culture 30 days, taken out from basin, extract all blades in middle and lower part, the root system of whole plant is then totally immersed into special taking root
Agent 1~2 minute, special root-growing agent is the aqueous solution (sterilized water) containing indolebutyric acid (IBA) and Nafusaku, and wherein IBA's is dense
It is 1.0g/L to spend, and the mass ratio of IBA and Nafusaku is 20:1, then by the whole root system of plant with preservative film wrap up (10 plants/
Prick), obtain blueberry tissue culture bare-root seeding to be planted.
(2) seedbed makes：Crop field deep ploughing is digged, soil improvement to pH value is 4.5~5.0 by addition Cosan, is obtained
Blueberry seedbed, sets blueberry seedling cave on the blueberry seedbed；The height in blueberry seedbed is 60cm, width is 3.0m, the indigo plant
The hill spacing in certain kind of berries seedling cave is 30cm, line-spacing is 35cm, depth is 25~30cm.
(3) seedling cave addition substrate preparation：By mushroom slag, biogas residue, pine needle, pine tree class bark wood chip and chicken manure, cow dung, pig manure etc.
The fermentation of animal dung batch mixing pile-turning obtains matrix, then adds insecticide and bactericide, after stirring, from phosphoric acid, ferrous sulfate
The pH value for adjusting matrix is 4.5~5.5, and with covered rearing with plastic film 8~10 days, obtains blueberry seedling cave and add matrix；The base
The parameter of matter is as follows：Mass percentage content >=45% of organic matter, matrix particle diameter≤5mm, matrix unit weight is 0.15~
0.50g/cm3、N+P2O5+K2O total nutrients in terms of butt >=1%, EC values≤1.0ms/cm, moisture content≤30%；The insecticide
It is potassium permanganate with bactericide, when using, is configured to the potassium permanganate solution of mass percent 0.1~0.5%, is sprayed onto base
In matter, until irrigating.
(4) transplant：The self-control matrix that thickness is 10~15cm is first sprinkled in seedling cave before transplanting, covering thickness in matrix again is
The soil layer of 2~4cm, spacing in the rows 30cm, line-spacing 35cm, blueberry seedling is placed on the soil layer in planting pit vertically, and nursery stock surrounding is again
Maintained an equal level to ground with soil covering, the final depth of planting is covering 2~4cm of seedling root, then root water is poured around nursery stock, fixed
Root water is the compound sodium nitrophenolate aqueous solution of mass percent concentration 0.003~0.009%, and every plant of blueberry seedling pours 1 liter of root water.
(5) managed after planting：Later stage proceed by water, weeding, the field management such as prevention and control of plant diseases, pest control work.Water and use water
Fertile integrated management technology, weeding artificial weeding, the prevention and control of plant diseases, pest control is made between using, installs biology, the physics such as blacklight trap
Method prevents and treats blueberry pest and disease damage.
After nursery stock of the present invention ground is planted 1 year, seedling height is up to 0.4~0.6m, ground diameter >=0.6cm；Root system threadiness, uniformly
Distribution, up to 15~17cm, at most, the Fine Root Biomass of root footpath 2mm is always biological for the root quantity of root footpath≤1mm for Root Distribution scope
The 84%~92% of amount, root system is distributed in the soil layer of 0~25cm to be concentrated the most；Transplanting survival rate >=90%.
Claims (7)
1. a kind of blueberry tissue culture bare-root seeding field-transplanting method, it is characterised in that comprise the steps of：
(1) nursery stock pretreatment：The blueberry tissue culture bottle seedling for breeding for 3~5 generations is cultivated 90~120 days in outside sprout-cultivating-bottle rear chamber, after
Continuous outside scenery takes out after 30~45 days from basin, extracts all blades in middle and lower part, then all soaks the whole root system of plant
Enter special root-growing agent and taken out after 1~2 minute to obtain blueberry tissue culture bare-root seeding to be planted；
(2) seedbed makes：Crop field deep ploughing is digged, soil improvement to pH value is 4.5~5.0 by addition Cosan, obtains blueberry
Seedbed, sets blueberry seedling cave on the blueberry seedbed；
(3) seedling cave addition substrate preparation：By the livestock and poultry such as mushroom slag, biogas residue, pine needle, pine tree class bark wood chip and chicken manure, cow dung, pig manure
The fermentation of excrement batch mixing pile-turning obtains matrix, then adds insecticide and bactericide, after stirring, from phosphoric acid, ferrous sulfate regulation
The pH value of matrix is 4.5~5.5, and with covered rearing with plastic film 8~10 days, obtains blueberry seedling cave and add matrix；
(4) transplant：After the blueberry seedling cave addition matrix obtained in step (3) is sprinkled in the blueberry seedling cave obtained in step (2)
Overlying soil, the blueberry tissue culture bare-root seeding obtained in step (1) is placed on the soil layer vertically, and surrounding is covered extremely with soil again
Maintain an equal level with ground, then root water is poured around the blueberry tissue culture bare-root seeding；
(5) managed after planting：The field management such as the later stage watered, weeding, prevention and control of plant diseases, pest control work.
2. blueberry tissue culture bare-root seeding field-transplanting method according to claim 1, it is characterised in that：It is described in step (1)
Root-growing agent is the aqueous solution containing indolebutyric acid and Nafusaku, wherein the concentration of IBA be 0.8~l.0g/L, IBA and Nafusaku
Mass ratio be 18:1~20:1.
3. blueberry tissue culture bare-root seeding field-transplanting method according to claim 1, it is characterised in that：It is described in step (1)
The intensity of illumination of indoor culture is 20001x~30001x, cultivation temperature is 22 ± 3 DEG C.
4. blueberry tissue culture bare-root seeding field-transplanting method according to claim 1, it is characterised in that：It is described in step (2)
The height in blueberry seedbed is that 50~60cm, width are 2.5~3.0m, and the hill spacing in the blueberry seedling cave is 20~30cm, line-spacing is
35~40cm, depth are 25~30cm.
5. blueberry tissue culture bare-root seeding field-transplanting method according to claim 1, it is characterised in that：It is described in step (3)
The parameter of matrix is as follows：Mass percentage content >=45% of organic matter, matrix particle diameter≤5mm, matrix unit weight is 0.15~
0.50g/cm3、N+P2O5+K2O total nutrients in terms of butt >=1%, EC values≤1.0ms/cm, moisture content≤30%.
6. blueberry tissue culture bare-root seeding field-transplanting method according to claim 1, it is characterised in that：It is described in step (3)
Insecticide and bactericide are potassium permanganate, when using, are configured to the potassium permanganate solution of mass percent 0.1~0.5%, are sprayed
It is spread across in matrix, until irrigating.
7. blueberry tissue culture bare-root seeding field-transplanting method according to claim 1, it is characterised in that：It is described in step (4)
The addition thickness of blueberry seedling cave addition matrix is 10~15cm；The thickness of the layer is 2~4cm；The root water is quality
The compound sodium nitrophenolate aqueous solution of percent concentration 0.003~0.009%, every plant of blueberry seedling pours 1 liter of root water.
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| CN201710069205.9A CN106888903A (en) | 2017-02-08 | 2017-02-08 | A kind of blueberry tissue culture bare-root seeding field-transplanting method |
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| CN201710069205.9A CN106888903A (en) | 2017-02-08 | 2017-02-08 | A kind of blueberry tissue culture bare-root seeding field-transplanting method |
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| CN106888903A true CN106888903A (en) | 2017-06-27 |
Family
ID=59198109
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| CN201710069205.9A Pending CN106888903A (en) | 2017-02-08 | 2017-02-08 | A kind of blueberry tissue culture bare-root seeding field-transplanting method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111386951A (en) * | 2020-03-23 | 2020-07-10 | 五指山南天蓝莓嘉宝果种植专业合作社 | Blueberry planting method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101707979A (en) * | 2009-11-30 | 2010-05-19 | 广西壮族自治区甘蔗研究所 | Sugarcane tissue-culture-seedling bare-seedling field-transplanting method |
| CN101869024A (en) * | 2009-04-21 | 2010-10-27 | 山东高端投资有限公司 | Non-polluted planting method of high blueberry |
| CN101904286A (en) * | 2009-06-05 | 2010-12-08 | 董文卓 | Method for growing blueberry |
| CN102511259A (en) * | 2011-11-23 | 2012-06-27 | 金寨县大别山林艺植物科技开发有限公司 | Blueberry planting method |
| CN104719163A (en) * | 2015-03-25 | 2015-06-24 | 温州科技职业学院 | Method for increasing acclimatization planting percent of blueberry tissue culture test-tube seedlings |
-
2017
- 2017-02-08 CN CN201710069205.9A patent/CN106888903A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101869024A (en) * | 2009-04-21 | 2010-10-27 | 山东高端投资有限公司 | Non-polluted planting method of high blueberry |
| CN101904286A (en) * | 2009-06-05 | 2010-12-08 | 董文卓 | Method for growing blueberry |
| CN101707979A (en) * | 2009-11-30 | 2010-05-19 | 广西壮族自治区甘蔗研究所 | Sugarcane tissue-culture-seedling bare-seedling field-transplanting method |
| CN102511259A (en) * | 2011-11-23 | 2012-06-27 | 金寨县大别山林艺植物科技开发有限公司 | Blueberry planting method |
| CN104719163A (en) * | 2015-03-25 | 2015-06-24 | 温州科技职业学院 | Method for increasing acclimatization planting percent of blueberry tissue culture test-tube seedlings |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111386951A (en) * | 2020-03-23 | 2020-07-10 | 五指山南天蓝莓嘉宝果种植专业合作社 | Blueberry planting method |
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Application publication date: 20170627 |