CN103535283A - Potato virus-free seedling tissue culture propagation method - Google Patents
Potato virus-free seedling tissue culture propagation method Download PDFInfo
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- CN103535283A CN103535283A CN201310534828.0A CN201310534828A CN103535283A CN 103535283 A CN103535283 A CN 103535283A CN 201310534828 A CN201310534828 A CN 201310534828A CN 103535283 A CN103535283 A CN 103535283A
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Abstract
The invention discloses a potato virus-free seedling tissue culture propagation method which relates to the propagation technology of potatoes and belongs to the technical filed of agriculture planting. The method is characterized by comprising the following steps: performing fine selection, performing accelerating germination, performing seed airing, and inoculating aired seeds in a germination culture medium for cultivation and growth, wherein the accelerating germination and sowing are performed at the temperature being 15-20 DEG C; the method has the beneficial effects that compared with a formula based on an MS, a formula in the method is small in quantity of constitution chemical substances, low in cost, and convenient to prepare. By using the method disclosed by the invention, potato virus-free seedlings can be propagated in an opening environment, and the characteristics that the propagation speed is high, the production cost is low, the popularization and the application are easy, and the like can be realized.
Description
Technical field
The present invention relates to the propagation technique of potato seed, belong to agricultural planting technique field, specifically a kind of potato virus-free plantlet tissue culture propagation method.
Background technology
Potato is one of traditional advantage crops of Dingxi, the stable development of potato industry, and cultivated area and benefit improve year by year and have become one of the main Potato Industry of the whole province base.Whole city's potato sowing area reaches 1.8 ten thousand hectares, and cultivated area accounts for 25% of the crop seeding gross area, on average produces bright potato 35250kg/ hectare, and total producing reaches 63,450 ten thousand kilograms.Through construction and development in a few years; whole city's potato planting has formed scale; intensive general layout; formed and take cold and damp district, frontier passes and mountains as main potato seed multiplication plot, mountain area, the central and north is that main late-maturing high starch commodity potato production base and warm river valley Chuan Qu is that main cooked food morning is used type commodity potato production base.Potato stock breeding basically forms, from autonomous seed selection, organize detoxifying fast breeding, greenhouse cuttage, the cultivation of net canopy, original seed to breed the integral framework of I and II production of seed stock.Summed up there is single ridge duplicate rows ridge culture technology of applicable Dingxi terraced fields characteristic, whole potato hole kind technology, film-mulching technique, balance fertilizing, integrated pest prevention and control and storage technique measure, output and benefit are obviously increased.Potato processing industry is flourish, has further extended potato industrialization chain.Whole city family more than 200 of built all kinds of large, medium and small processing enterprises, 5000 tons of year smart starch of production.
At present, although the development of whole city's Potato Industry is very fast, can't adapt to the new change in the style of economic growth.Be mainly manifested in the following aspects: the one, production insufficient investment affects output; The 2nd, seed potato scarcity of resources, kind is upgraded slow, and particularly high-quality specific breed is few, and cultivated area is little; The 3rd, potato seed quality testing system is unsound, and detection method means fall behind, and lack effective supervision and management; The 4th, deep processing industry and production development are uneven, and industry chain (supply chain) is short, and proportion is not enough, can not be well production development service; The 5th, export trade foreign trade degree is low, and propaganda strength is bad, does not form independent brand, is restricting the expansion in market.
The modes of reproduction of potato detoxicating cuvette seedling mainly contains two kinds at present, a kind of is that potato detoxicating cuvette seedling is directly bred in the matrix such as vermiculite, perlite, sand, sawdust, mountain base soil, and this method repoductive time is long, and survival rate is low, knot potato is few, and easy bacteria infection; Another kind is that potato detoxicating cuvette seedling is bred in liquid, this method reproduction speed is fast, survival rate is high, knot potato is many, but the nutrient solution of potato virus-free plantlet liquid training is at present used MS for basic formula substantially, cause like this production cost high, cause potato virus-free plantlet liquid training technology to be difficult to apply.
Developing Sustainable Agriculture, is global common recognition and trend, and he will be towards modernization, intensive, ensured sustained development, agriculture industrialization future development.Modern agriculture is the scientific agricultural arming with modern science and technology, has represented the integral level of agricultural development; Intensive agriculture has represented the developing direction of agricultural under certain economy, resource and technical conditions; Sustainable agriculture has represented agriculture developing goal; Agriculture industrialization is the innovation of chemurgy and rural area management style and organization system, has represented the approach of agricultural to modernization future development.
Summary of the invention
The object of the present invention is to provide a kind of potato virus-free plantlet tissue culture propagation method, solve the high problem of nutrient solution cost compare of current potato virus-free plantlet liquid training, the nutrient solution of the potato virus-free plantlet liquid training that a kind of cost is low is provided.
For achieving the above object, a kind of potato virus-free plantlet tissue culture propagation method of the present invention, is characterized in that:
One, selected processing: selection seed potato is explant, broadcast first 20 days potato seed is gone out to cellar for storing things, selected, select the obvious potato type rule of varietal characteristic, epidermis is smooth, the young age that the eye depth is consistent and strong age stem tuber plant, eliminate that potato type is irregular, coarse aging and eye protrudes, broken head is chapped, color of the leather dimness etc. has non-healthy sign the potato piece of epidermis;
Two, vernalization and basking seeds: broadcast and front seed potato is accessed to incubation growth on germination medium; The culture medium prescription using is: KNO
32000～2200mg/L, KH
2pO
4400～550mg/L, (NH
4) 2SO
4400～500mg/L, CaCl
2400～500mg/L, EDTA-Na
250～60mg/L, FeSO450～60mg/L, B940～60mg/L, NAA0.5～1.0mg/L, 6-BA0.5～1.0mg/L, coconut palm breast 100ml/L, mannitol 1g/L, active 0.5g/L, sucrose 25g/L, agar 3g/L nutrient solution are prepared with sterile water; Plant and set the selected vernalization under 15～20 ℃ of conditions that is placed on, when the most of eye of potato piece sprouts, reject rot potato and the thin bud potato of length, put in the sun and bask seeds, make young shoot turn green;
Three, potato seed stripping and slicing and processing: the small seed potato when the heavy 30g of potato left and right, whole photo sowing, plants the above stripping and slicing sowing of the heavy 70g of piece; Sow front 1～2d and carry out stripping and slicing, each stripping and slicing is containing 2～3 eyes;
Four, sowing: with 20～25d before late frost, sowing in time when 10cm soil temperature reaches 7～8 ℃.
The EC of described nutrient solution is that 0.5～0.9, pH value is 5.8～6.2.
A kind of potato virus-free plantlet tissue culture propagation method of the present invention, its beneficial effect is: formula of the present invention and MS are that basic formula is compared, and constitutional chemistry material is few, and cost is low, and preparation is convenient.Use the present invention can make potato virus-free plantlet breed under open condition, have reproduction speed fast, production cost is low, the feature such as easily applies.Use the present invention to have significant effect in strong sprout to potato virus-free plantlet; in 7 days, stem slightly reaches 1.6～2.3mm; root system is between 16～22; and there are many lateral roots; all healthy vigorous growth of energy of potato virus-free plantlet; after being transplanted to net canopy and land for growing field crops, survival rate reaches more than 95%, for scale, low-cost production micro potato provide technical guarantee.
Embodiment
Following examples are that the present invention further goes out to set forth.
Embodiment 1
One, selected processing: selection seed potato is explant, broadcast first 20 days potato seed is gone out to cellar for storing things, selected, select the obvious potato type rule of varietal characteristic, epidermis is smooth, the young age that the eye depth is consistent and strong age stem tuber plant, eliminate that potato type is irregular, coarse aging and eye protrudes, broken head is chapped, color of the leather dimness etc. has non-healthy sign the potato piece of epidermis;
Two, vernalization and basking seeds: broadcast and front seed potato is accessed to incubation growth on germination medium; The culture medium prescription using is: KNO
32000mg/L, KH
2pO
4400mg/L, (NH
4) 2SO4400mg/L, CaCl
2400mg/L, EDTA-Na
250mg/L, FeSO
450mg/L, B940mg/L, NAA0.5mg/L, 6-BA0.5mg/L, coconut palm breast 100ml/L, mannitol 1g/L, active 0.5g/L, sucrose 25g/L, agar 3g/L nutrient solution are prepared with sterile water; Plant and set the selected vernalization under 15 ℃ of conditions that is placed on, when the most of eye of potato piece sprouts, reject rot potato and the thin bud potato of length, put in the sun and bask seeds, make young shoot turn green; The EC of described nutrient solution is that 0.5, pH value is 5.8;
Three, potato seed stripping and slicing and processing: the small seed potato when the heavy 30g of potato left and right, whole photo sowing, plants the above stripping and slicing sowing of the heavy 70g of piece; Before sowing, 1d carries out stripping and slicing, and each stripping and slicing is containing 2 eyes;
Four, sowing: with 20d before late frost, sowing in time when 10cm soil temperature reaches 7 ℃.
Embodiment 2
One, selected processing: selection seed potato is explant, broadcast first 20 days potato seed is gone out to cellar for storing things, selected, select the obvious potato type rule of varietal characteristic, epidermis is smooth, the young age that the eye depth is consistent and strong age stem tuber plant, eliminate that potato type is irregular, coarse aging and eye protrudes, broken head is chapped, color of the leather dimness etc. has non-healthy sign the potato piece of epidermis;
Two, vernalization and basking seeds: broadcast and front seed potato is accessed to incubation growth on germination medium; The culture medium prescription using is: KNO
32200mg/L, KH
2pO
4550mg/L, (NH
4) 2SO
4500mg/L, CaCl
2500mg/L, EDTA-Na
260mg/L, FeSO
460mg/L, B960mg/L, NAA1.0mg/L, 6-BA1.0mg/L, coconut palm breast 100ml/L, mannitol 1g/L, active 0.5g/L, sucrose 25g/L, agar 3g/L nutrient solution are prepared with sterile water, and the EC of nutrient solution is that 0.5～0.9, pH value is 5.8～6.2; Plant and set the selected vernalization under 20 ℃ of conditions that is placed on, when the most of eye of potato piece sprouts, reject rot potato and the thin bud potato of length, put in the sun and bask seeds, make young shoot turn green;
Three, potato seed stripping and slicing and processing: the small seed potato when the heavy 30g of potato left and right, whole photo sowing, plants the above stripping and slicing sowing of the heavy 70g of piece; Before sowing, 2d carries out stripping and slicing, and each stripping and slicing is containing 3 eyes;
Four, sowing: with 25d before late frost, sowing in time when 10cm soil temperature reaches 8 ℃.
Embodiment 3
One, selected processing: selection seed potato is explant, broadcast first 20 days potato seed is gone out to cellar for storing things, selected, select the obvious potato type rule of varietal characteristic, epidermis is smooth, the young age that the eye depth is consistent and strong age stem tuber plant, eliminate that potato type is irregular, coarse aging and eye protrudes, broken head is chapped, color of the leather dimness etc. has non-healthy sign the potato piece of epidermis;
Two, vernalization and basking seeds: broadcast and front seed potato is accessed to incubation growth on germination medium; The culture medium prescription using is: KNO
32100mg/L, KH
2pO
4500mg/L, (NH
4) 2SO
4450mg/L, CaCl
2450mg/L, EDTA-Na
255mg/L, FeSO
455mg/L, B950mg/L, NAA0.7mg/L, 6-BA0.7mg/L, coconut palm breast 100ml/L, mannitol 1g/L, active 0.5g/L, sucrose 25g/L, agar 3g/L nutrient solution are prepared with sterile water, and the EC of nutrient solution is that 0.5～0.9, pH value is 5.8～6.2; Plant and set the selected vernalization under 17 ℃ of conditions that is placed on, when the most of eye of potato piece sprouts, reject rot potato and the thin bud potato of length, put in the sun and bask seeds, make young shoot turn green;
Three, potato seed stripping and slicing and processing: the small seed potato when the heavy 30g of potato left and right, whole photo sowing, plants the above stripping and slicing sowing of the heavy 70g of piece; Before sowing, 2d carries out stripping and slicing, and each stripping and slicing is containing 2 eyes;
Four, sowing: with 22d before late frost, sowing in time when 10cm soil temperature reaches 7 ℃.
Claims (2)
1. a potato virus-free plantlet tissue culture propagation method, is characterized in that:
1. selected processing: selection seed potato is explant, broadcast first 20 days potato seed is gone out to cellar for storing things, selected, select the obvious potato type rule of varietal characteristic, epidermis is smooth, the young age that the eye depth is consistent and strong age stem tuber plant, eliminate that potato type is irregular, coarse aging and eye protrudes, broken head is chapped, color of the leather dimness has non-healthy sign the potato piece of epidermis;
2. vernalization with bask seeds: broadcast and front seed potato accessed to incubation growth on germination medium; The culture medium prescription using is: KNO
32000～2200mg/L, KH
2pO
4400～550mg/L, (NH
4) 2SO
4400～500mg/L, CaCl
2400～500mg/L, EDTA-Na
250～60mg/L, FeSO450～60mg/L, B940～60mg/L, NAA0.5～1.0mg/L, 6-BA0.5～1.0mg/L, coconut palm breast 100ml/L, mannitol 1g/L, active 0.5g/L, sucrose 25g/L, agar 3g/L nutrient solution are prepared with sterile water; Potato seed is selected is placed on vernalization under 15～20 ℃ of conditions, when the most of eye of potato piece sprouts, rejects rot potato and long thin bud potato, puts in the sun and basks seeds, and makes young shoot turn green;
3. potato seed stripping and slicing and processing: the small seed potato of the heavy 30g of potato left and right, whole photo sowing, plants the above stripping and slicing sowing of the heavy 70g of piece; Sow front 1～2d and carry out stripping and slicing, each stripping and slicing is containing 2～3 eyes;
4. sowing: with 20～25d before late frost, sowing in time when 10cm soil temperature reaches 7～8 ℃.
2. a kind of potato virus-free plantlet tissue culture propagation method as claimed in claim 1, is characterized in that: the EC of described nutrient solution is that 0.5～0.9, pH value is 5.8～6.2.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103947552A (en) * | 2014-04-28 | 2014-07-30 | 内蒙古嘉恒农业科技有限责任公司 | Potato virus-free bottle seedling culture medium and preparation method thereof |
| CN105532449A (en) * | 2015-12-04 | 2016-05-04 | 徐伟明 | Virus free culture medium of potato |
| CN105532480A (en) * | 2016-02-16 | 2016-05-04 | 江苏强农农业技术服务有限公司 | Potato anther induction medium and anther-culture breeding method |
| CN106386056A (en) * | 2016-08-31 | 2017-02-15 | 山西省农业科学院高寒区作物研究所 | Cultivation method for potatoes |
| CN107182781A (en) * | 2017-05-03 | 2017-09-22 | 蚌埠市乔峰农业蔬菜专业合作社 | A kind of seed potato poison-removing method |
| CN109804752A (en) * | 2018-11-15 | 2019-05-28 | 李涛 | A kind of entire mechanization production method of potato matching of agricultural machinery and agriculture |
| CN112273227A (en) * | 2020-10-15 | 2021-01-29 | 永州职业技术学院 | Culture medium for improving survival rate of explants and preparation method and application thereof |
| CN114467752A (en) * | 2022-02-21 | 2022-05-13 | 成都久森农业科技有限公司 | Method for culturing potato tissue culture seedlings at constant temperature by using underground water |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103947552A (en) * | 2014-04-28 | 2014-07-30 | 内蒙古嘉恒农业科技有限责任公司 | Potato virus-free bottle seedling culture medium and preparation method thereof |
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| CN105532480A (en) * | 2016-02-16 | 2016-05-04 | 江苏强农农业技术服务有限公司 | Potato anther induction medium and anther-culture breeding method |
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| CN107182781A (en) * | 2017-05-03 | 2017-09-22 | 蚌埠市乔峰农业蔬菜专业合作社 | A kind of seed potato poison-removing method |
| CN109804752A (en) * | 2018-11-15 | 2019-05-28 | 李涛 | A kind of entire mechanization production method of potato matching of agricultural machinery and agriculture |
| CN112273227A (en) * | 2020-10-15 | 2021-01-29 | 永州职业技术学院 | Culture medium for improving survival rate of explants and preparation method and application thereof |
| CN114467752A (en) * | 2022-02-21 | 2022-05-13 | 成都久森农业科技有限公司 | Method for culturing potato tissue culture seedlings at constant temperature by using underground water |
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Application publication date: 20140129 |